Rh1AG330300

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1A
OG0018075
Physical Location & Seq
Forward (+)
56311453 .. 56312025
573 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1AG330300.1

Sequence Viewer

Length: 573 bp
ATGGCCATGCTAACCATAGCTTGCAGCTTAATCCTGCTAATCACCACCACCATAGACCACTCTTTTGCAGCTGCTCGAAGCTTAAGCAATTCAAACCCATCACACCCTCACAACCACCATCACAAACTCACATTCCTAATGAGAGATGTGCTCATGAATGTGACTCAACCCTCATCCAAGACCAAACCAGCAACCACCAAAGTGACTAGTCCTCAGCTGCCATTCTCAAAACCACTAGGACTATTTCCTCCAAATGGAGGAGTCCCCATCTCAGAAATCAATCCCATGCATACCACTACTTCTCCAGTTTCTGGAGTCTCCACTCAAACGTTAGATTTATCCAGCATTGGACTATTTTTTCCTGCTAGCGCCACACTGCAAGAACTGGAGTTTGGGGAAGTAACCGTGATCGATGAGGATATATTCGAAACTTTTACTTCAGGGTATGGTTCACTGGTCATTGGAAAAGCACAGGGGATATATGTTGCAAGCTCTGTAGATGGTAGTAGCCACATGATGGCCATGACTGCACATTTTGCTAATAGTAAATTTAAGGACGGATTGACTGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

190

Amino Acids

20.28

Weight (kDa)

6.1

Isoelectric Point (pI)

40.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 125 - 186 2.9e-09 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018075)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20990
malus_domestica MD07G1189100.v1.1
prunus_persica Prupe.2G226600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365381
rosa_laevigata RLG00000027427
rosa_roxburghii Rroxscaffold_4G00291270
rosa_rugosa Rorug01G0321500
rosa_samantha Rh1AG330300 Rh1BG291900 Rh1DG323500
rosa_wichuraiana Rw1G029290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 311, 517
AclI AACGTT 1 cut(s) 329
AcoI YGGCCR 2 cut(s) 3, 519
AcsI RAATTY 1 cut(s) 548
AcuI CTGAAG 1 cut(s) 423
AfiI CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
AflII CTTAAG 1 cut(s) 82
AgsI TTSAA 1 cut(s) 93
AhlI ACTAGT 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AleI CACNNNNGTG 1 cut(s) 200
AluBI AGCT 6 cut(s) 20, 27, 71, 81, 217, 492
AluI AGCT 6 cut(s) 20, 27, 71, 81, 217, 492
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 1 cut(s) 322
AlwNI CAGNNNCTG 1 cut(s) 311
AoxI GGCC 2 cut(s) 3, 519
ApeKI GCWGC 4 cut(s) 24, 68, 71, 217
ApoI RAATTY 1 cut(s) 548
ArsI GACNNNNNNTTYG 2 cut(s) 47, 79
AspLEI GCGC 1 cut(s) 371
AsuHPI GGTGA 1 cut(s) 34
AsuII TTCGAA 1 cut(s) 426
AsuNHI GCTAGC 1 cut(s) 365
BalI TGGCCA 2 cut(s) 5, 521
Bbv12I GWGCWC 1 cut(s) 153
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 4 cut(s) 36, 58, 80, 204
BccI CCATC 5 cut(s) 106, 126, 275, 494, 511
BcoDI GTCTC 1 cut(s) 322
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 3 cut(s) 207, 236, 366
BfmI CTRYAG 1 cut(s) 495
BfoI RGCGCY 1 cut(s) 372
BfrI CTTAAG 1 cut(s) 82
BisI GCNGC 4 cut(s) 25, 69, 72, 218
BlsI GCNGC 4 cut(s) 26, 70, 73, 219
BmtI GCTAGC 1 cut(s) 369
BplI GAGNNNNNCTC 2 cut(s) 135, 167
BpmI CTGGAG 3 cut(s) 288, 333, 407
Bpu10I CCTNAGC 1 cut(s) 213
Bpu14I TTCGAA 1 cut(s) 426
Bsa29I ATCGAT 1 cut(s) 411
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
Bse1I ACTGG 3 cut(s) 305, 390, 459
BseCI ATCGAT 1 cut(s) 411
BseGI GGATG 1 cut(s) 173
BseLI CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
BseMII CTCAG 2 cut(s) 227, 285
BseNI ACTGG 3 cut(s) 305, 390, 459
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 4 cut(s) 36, 58, 80, 204
BsgI GTGCAG 1 cut(s) 513
BshFI GGCC 2 cut(s) 5, 521
BshVI ATCGAT 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 153
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
BsmAI GTCTC 1 cut(s) 322
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 2 cut(s) 5, 521
Bsp119I TTCGAA 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 153
Bsp143I GATC 1 cut(s) 408
BspANI GGCC 2 cut(s) 5, 521
BspCNI CTCAG 2 cut(s) 226, 284
BspDI ATCGAT 1 cut(s) 411
BspHI TCATGA 1 cut(s) 153
BspOI GCTAGC 1 cut(s) 369
BspT104I TTCGAA 1 cut(s) 426
BspTI CTTAAG 1 cut(s) 82
BsrI ACTGG 3 cut(s) 305, 390, 459
BssMI GATC 1 cut(s) 408
Bst4CI ACNGT 1 cut(s) 406
BstAFI CTTAAG 1 cut(s) 82
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 426
BstC8I GCNNGC 3 cut(s) 22, 367, 490
BstDEI CTNAG 2 cut(s) 213, 271
BstF5I GGATG 1 cut(s) 173
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 1 cut(s) 411
BstMAI GTCTC 1 cut(s) 322
BstMBI GATC 1 cut(s) 408
BstMWI GCNNNNNNNGC 2 cut(s) 527, 536
BstSFI CTRYAG 1 cut(s) 495
BstV1I GCAGC 4 cut(s) 36, 58, 80, 204
Bsu15I ATCGAT 1 cut(s) 411
BsuRI GGCC 2 cut(s) 5, 521
BsuTUI ATCGAT 1 cut(s) 411
BtsCI GGATG 1 cut(s) 173
BtsI GCAGTG 1 cut(s) 374
BtsIMutI CAGTG 2 cut(s) 374, 452
Cac8I GCNNGC 3 cut(s) 22, 367, 490
CaiI CAGNNNCTG 1 cut(s) 311
CciI TCATGA 1 cut(s) 153
CfoI GCGC 1 cut(s) 371
ClaI ATCGAT 1 cut(s) 411
CviAII CATG 5 cut(s) 7, 154, 286, 514, 523
CviJI RGCY 9 cut(s) 5, 20, 27, 71, 81, 217, 492, 510, 521
CviKI_1 RGCY 9 cut(s) 5, 20, 27, 71, 81, 217, 492, 510, 521
DdeI CTNAG 2 cut(s) 213, 271
DpnI GATC 1 cut(s) 410
DpnII GATC 1 cut(s) 408
EaeI YGGCCR 2 cut(s) 3, 519
Eco57I CTGAAG 1 cut(s) 423
EcoT22I ATGCAT 1 cut(s) 291
FaeI CATG 5 cut(s) 10, 157, 289, 517, 526
FaqI GGGAC 1 cut(s) 248
FatI CATG 5 cut(s) 6, 153, 285, 513, 522
Fnu4HI GCNGC 4 cut(s) 25, 69, 72, 218
FokI GGATG 1 cut(s) 160
Fsp4HI GCNGC 4 cut(s) 25, 69, 72, 218
FspBI CTAG 3 cut(s) 207, 236, 366
GlaI GCGC 1 cut(s) 370
GluI GCNGC 4 cut(s) 25, 69, 72, 218
GsuI CTGGAG 3 cut(s) 288, 333, 407
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 2 cut(s) 5, 521
HhaI GCGC 1 cut(s) 371
Hin1II CATG 5 cut(s) 10, 157, 289, 517, 526
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 3 cut(s) 163, 261, 315
HphI GGTGA 1 cut(s) 34
Hpy166II GTNNAC 1 cut(s) 452
Hpy188I TCNGA 1 cut(s) 274
Hpy188III TCNNGA 2 cut(s) 154, 312
Hpy8I GTNNAC 1 cut(s) 452
HpyCH4III ACNGT 1 cut(s) 406
HpyCH4IV ACGT 1 cut(s) 329
HpyCH4V TGCA 6 cut(s) 24, 68, 289, 379, 488, 530
HpyF10VI GCNNNNNNNGC 2 cut(s) 527, 536
HpyF3I CTNAG 2 cut(s) 213, 271
HpySE526I ACGT 1 cut(s) 329
Hsp92II CATG 5 cut(s) 10, 157, 289, 517, 526
HspAI GCGC 1 cut(s) 369
Kzo9I GATC 1 cut(s) 408
Lsp1109I GCAGC 4 cut(s) 36, 58, 80, 204
MaeI CTAG 3 cut(s) 207, 236, 366
MaeII ACGT 1 cut(s) 329
MaeIII GTNAC 3 cut(s) 160, 202, 400
MalI GATC 1 cut(s) 410
MboI GATC 1 cut(s) 408
MhlI GDGCHC 1 cut(s) 153
MlsI TGGCCA 2 cut(s) 5, 521
MluCI AATT 2 cut(s) 88, 548
MluNI TGGCCA 2 cut(s) 5, 521
MlyI GAGTC 3 cut(s) 157, 270, 324
MnlI CCTC 6 cut(s) 117, 181, 222, 251, 258, 409
Mox20I TGGCCA 2 cut(s) 5, 521
Mph1103I ATGCAT 1 cut(s) 291
MscI TGGCCA 2 cut(s) 5, 521
MseI TTAA 3 cut(s) 29, 83, 552
MslI CAYNNNNRTG 2 cut(s) 158, 200
Msp20I TGGCCA 2 cut(s) 5, 521
MspA1I CMGCKG 2 cut(s) 71, 217
MspCI CTTAAG 1 cut(s) 82
MwoI GCNNNNNNNGC 2 cut(s) 527, 536
NdeII GATC 1 cut(s) 408
NheI GCTAGC 1 cut(s) 365
NlaIII CATG 5 cut(s) 10, 157, 289, 517, 526
NmuCI GTSAC 2 cut(s) 160, 202
NsiI ATGCAT 1 cut(s) 291
NspV TTCGAA 1 cut(s) 426
OliI CACNNNNGTG 1 cut(s) 200
PagI TCATGA 1 cut(s) 153
PflMI CCANNNNNTGG 2 cut(s) 311, 517
PkrI GCNGC 4 cut(s) 26, 70, 73, 219
PleI GAGTC 3 cut(s) 157, 269, 323
PpsI GAGTC 3 cut(s) 157, 269, 323
Psp1406I AACGTT 1 cut(s) 329
PstNI CAGNNNCTG 1 cut(s) 311
PvuII CAGCTG 2 cut(s) 71, 217
RseI CAYNNNNRTG 2 cut(s) 158, 200
SaqAI TTAA 3 cut(s) 29, 83, 552
SatI GCNGC 4 cut(s) 25, 69, 72, 218
Sau3AI GATC 1 cut(s) 408
SchI GAGTC 3 cut(s) 157, 270, 324
SduI GDGCHC 1 cut(s) 153
SetI ASST 7 cut(s) 22, 29, 73, 83, 219, 332, 494
SfcI CTRYAG 1 cut(s) 495
SfuI TTCGAA 1 cut(s) 426
SmiMI CAYNNNNRTG 2 cut(s) 158, 200
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
SpeI ACTAGT 1 cut(s) 206
Sse9I AATT 2 cut(s) 88, 548
SspMI CTAG 3 cut(s) 207, 236, 366
TaaI ACNGT 1 cut(s) 406
TaiI ACGT 1 cut(s) 332
TaqI TCGA 3 cut(s) 76, 411, 426
TasI AATT 2 cut(s) 88, 548
Tru1I TTAA 3 cut(s) 29, 83, 552
Tru9I TTAA 3 cut(s) 29, 83, 552
TscAI CASTG 2 cut(s) 381, 459
TseFI GTSAC 2 cut(s) 160, 202
TseI GCWGC 4 cut(s) 24, 68, 71, 217
Tsp45I GTSAC 2 cut(s) 160, 202
TspDTI ATGAA 1 cut(s) 170
TspGWI ACGGA 1 cut(s) 573
TspRI CASTG 2 cut(s) 381, 459
Van91I CCANNNNNTGG 2 cut(s) 311, 517
Vha464I CTTAAG 1 cut(s) 82
XapI RAATTY 1 cut(s) 548
XspI CTAG 3 cut(s) 207, 236, 366
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.