Rh1DG323500

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1D
OG0018075
Physical Location & Seq
Forward (+)
54927064 .. 54927791
728 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1DG323500.1

Sequence Viewer

Length: 687 bp
ATGGCCATGATAACCATAACTTGCAGCTTAATCCTGCTAATCACCACCACCATAAACCACTCTTTTGCCGCTGCTCGAAGCTTAAGCAATTCAAACCCATCACACCCTCACAACCACCATCACAAACTCACATTCCTAATGAGAGATGTGCTCATGAATGTGACTCAACCCTCATCCAAGACCAAACCAGCAACCACCAAAGTGACTAGTCCTCAGCTGCCATTCTCAAAACCACTAGGACTATTTCCTCCAAATGGCGGAGTCCCCATCTCAGAAATCAATCCCATGCATACCACTACTTCTCCAGTTTCCGGAGTCTCCACTCAAACGCTAGATTTATCCGGCATTGGACTATTTTTTCCTGCTAGAGCCACACTCCAAGAACTGGAGTTTGGGGAAGTAACCGTGATCGATGAGGACATATTCGAAACTTTTACTTCAGGGTATGGATCACTGGTCATTGGAAAAGCACGGGGGATATATGTTGCTAGCTCTGAAGAAGGGAGTAGCCACATGATGGCCATGACTGCACATTTTGCTAATAGTCAATTTAAGGACGGATTGAGACTTTTCGGAGTTCATCGGAGGGATGTGCATGATGAATCTCATGTTTCTGTTATTGGTGGCATTGGAAAGTATGTGGGTACGTGCAAATGGCTATGCAACAGTACAGACAGTGAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

228

Amino Acids

24.66

Weight (kDa)

6.62

Isoelectric Point (pI)

40.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 126 - 213 8.8e-13 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018075)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20990
malus_domestica MD07G1189100.v1.1
prunus_persica Prupe.2G226600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365381
rosa_laevigata RLG00000027427
rosa_roxburghii Rroxscaffold_4G00291270
rosa_rugosa Rorug01G0321500
rosa_samantha Rh1AG330300 Rh1BG291900 Rh1DG323500
rosa_wichuraiana Rw1G029290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 385, 517
AccIII TCCGGA 1 cut(s) 311
AciI CCGC 2 cut(s) 69, 258
AclWI GGATC 1 cut(s) 457
AcoI YGGCCR 2 cut(s) 3, 519
AcuI CTGAAG 2 cut(s) 423, 516
AfaI GTAC 2 cut(s) 646, 670
AfiI CCNNNNNNNGG 5 cut(s) 254, 257, 311, 385, 517
AflII CTTAAG 1 cut(s) 82
AgsI TTSAA 1 cut(s) 93
AhlI ACTAGT 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AleI CACNNNNGTG 1 cut(s) 200
AluBI AGCT 4 cut(s) 27, 81, 217, 492
AluI AGCT 4 cut(s) 27, 81, 217, 492
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 2 cut(s) 322, 559
AlwI GGATC 1 cut(s) 457
Aor13HI TCCGGA 1 cut(s) 311
AoxI GGCC 2 cut(s) 3, 519
ApeKI GCWGC 3 cut(s) 24, 71, 217
AsuHPI GGTGA 1 cut(s) 34
AsuII TTCGAA 1 cut(s) 426
AsuNHI GCTAGC 1 cut(s) 488
BalI TGGCCA 2 cut(s) 5, 521
Bbv12I GWGCWC 1 cut(s) 153
BbvCI CCTCAGC 1 cut(s) 213
BbvI GCAGC 3 cut(s) 36, 58, 204
BccI CCATC 4 cut(s) 106, 126, 275, 511
BcoDI GTCTC 2 cut(s) 322, 559
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 5 cut(s) 207, 236, 332, 366, 489
BfrI CTTAAG 1 cut(s) 82
BisI GCNGC 4 cut(s) 25, 69, 72, 218
BlsI GCNGC 4 cut(s) 26, 70, 73, 219
BmtI GCTAGC 1 cut(s) 492
BplI GAGNNNNNCTC 4 cut(s) 135, 167, 360, 392
BpmI CTGGAG 2 cut(s) 288, 407
Bpu10I CCTNAGC 1 cut(s) 213
Bpu14I TTCGAA 1 cut(s) 426
Bsa29I ATCGAT 1 cut(s) 411
BsaAI YACGTR 1 cut(s) 648
BsaWI WCCGGW 1 cut(s) 311
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bsc4I CCNNNNNNNGG 5 cut(s) 254, 257, 311, 385, 517
Bse1I ACTGG 3 cut(s) 305, 390, 459
BseAI TCCGGA 1 cut(s) 311
BseCI ATCGAT 1 cut(s) 411
BseGI GGATG 2 cut(s) 173, 595
BseLI CCNNNNNNNGG 5 cut(s) 254, 257, 311, 385, 517
BseMII CTCAG 2 cut(s) 227, 285
BseNI ACTGG 3 cut(s) 305, 390, 459
BseXI GCAGC 3 cut(s) 36, 58, 204
BsgI GTGCAG 1 cut(s) 513
BshFI GGCC 2 cut(s) 5, 521
BshVI ATCGAT 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 2 cut(s) 312, 342
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 5 cut(s) 254, 257, 311, 385, 517
BsmAI GTCTC 2 cut(s) 322, 559
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 2 cut(s) 5, 521
Bsp119I TTCGAA 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 153
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 2 cut(s) 408, 449
BspACI CCGC 2 cut(s) 69, 258
BspANI GGCC 2 cut(s) 5, 521
BspCNI CTCAG 2 cut(s) 226, 284
BspDI ATCGAT 1 cut(s) 411
BspEI TCCGGA 1 cut(s) 311
BspHI TCATGA 1 cut(s) 153
BspOI GCTAGC 1 cut(s) 492
BspPI GGATC 1 cut(s) 457
BspT104I TTCGAA 1 cut(s) 426
BspTI CTTAAG 1 cut(s) 82
BsrI ACTGG 3 cut(s) 305, 390, 459
BssMI GATC 2 cut(s) 408, 449
Bst4CI ACNGT 3 cut(s) 406, 668, 677
BstAFI CTTAAG 1 cut(s) 82
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBAI YACGTR 1 cut(s) 648
BstBI TTCGAA 1 cut(s) 426
BstC8I GCNNGC 1 cut(s) 490
BstDEI CTNAG 2 cut(s) 213, 271
BstF5I GGATG 2 cut(s) 173, 595
BstKTI GATC 2 cut(s) 411, 452
BstMAI GTCTC 2 cut(s) 322, 559
BstMBI GATC 2 cut(s) 408, 449
BstMWI GCNNNNNNNGC 2 cut(s) 527, 536
BstV1I GCAGC 3 cut(s) 36, 58, 204
Bsu15I ATCGAT 1 cut(s) 411
BsuRI GGCC 2 cut(s) 5, 521
BsuTUI ATCGAT 1 cut(s) 411
BtsCI GGATG 2 cut(s) 173, 595
BtsIMutI CAGTG 2 cut(s) 452, 682
Cac8I GCNNGC 1 cut(s) 490
CciI TCATGA 1 cut(s) 153
ClaI ATCGAT 1 cut(s) 411
Csp6I GTAC 2 cut(s) 645, 669
CviAII CATG 7 cut(s) 7, 154, 286, 514, 523, 596, 608
CviJI RGCY 9 cut(s) 5, 27, 81, 217, 371, 492, 510, 521, 658
CviKI_1 RGCY 9 cut(s) 5, 27, 81, 217, 371, 492, 510, 521, 658
CviQI GTAC 2 cut(s) 645, 669
DdeI CTNAG 2 cut(s) 213, 271
DpnI GATC 2 cut(s) 410, 451
DpnII GATC 2 cut(s) 408, 449
EaeI YGGCCR 2 cut(s) 3, 519
EciI GGCGGA 1 cut(s) 273
Eco57I CTGAAG 2 cut(s) 423, 516
EcoT22I ATGCAT 1 cut(s) 291
FaeI CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
FaqI GGGAC 1 cut(s) 248
FatI CATG 7 cut(s) 6, 153, 285, 513, 522, 595, 607
Fnu4HI GCNGC 4 cut(s) 25, 69, 72, 218
FokI GGATG 2 cut(s) 160, 602
Fsp4HI GCNGC 4 cut(s) 25, 69, 72, 218
FspBI CTAG 5 cut(s) 207, 236, 332, 366, 489
GluI GCNGC 4 cut(s) 25, 69, 72, 218
GsuI CTGGAG 2 cut(s) 288, 407
HaeIII GGCC 2 cut(s) 5, 521
HapII CCGG 2 cut(s) 312, 342
Hin1II CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 4 cut(s) 163, 261, 315, 602
HpaII CCGG 2 cut(s) 312, 342
HphI GGTGA 1 cut(s) 34
Hpy188I TCNGA 4 cut(s) 274, 496, 575, 585
Hpy188III TCNNGA 2 cut(s) 154, 312
HpyAV CCTTC 1 cut(s) 494
HpyCH4III ACNGT 3 cut(s) 406, 668, 677
HpyCH4IV ACGT 1 cut(s) 647
HpyCH4V TGCA 6 cut(s) 24, 289, 530, 595, 651, 663
HpyF10VI GCNNNNNNNGC 2 cut(s) 527, 536
HpyF3I CTNAG 2 cut(s) 213, 271
HpySE526I ACGT 1 cut(s) 647
Hsp92II CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
Kpn2I TCCGGA 1 cut(s) 311
Kzo9I GATC 2 cut(s) 408, 449
LpnPI CCDG 9 cut(s) 47, 201, 318, 325, 355, 371, 375, 426, 440
Lsp1109I GCAGC 3 cut(s) 36, 58, 204
MaeI CTAG 5 cut(s) 207, 236, 332, 366, 489
MaeII ACGT 1 cut(s) 647
MaeIII GTNAC 3 cut(s) 160, 202, 400
MalI GATC 2 cut(s) 410, 451
MboI GATC 2 cut(s) 408, 449
MboII GAAGA 1 cut(s) 509
MhlI GDGCHC 1 cut(s) 153
MlsI TGGCCA 2 cut(s) 5, 521
MluCI AATT 2 cut(s) 88, 548
MluNI TGGCCA 2 cut(s) 5, 521
MlyI GAGTC 3 cut(s) 157, 270, 324
MnlI CCTC 6 cut(s) 117, 181, 222, 258, 409, 579
Mox20I TGGCCA 2 cut(s) 5, 521
Mph1103I ATGCAT 1 cut(s) 291
MroI TCCGGA 1 cut(s) 311
MscI TGGCCA 2 cut(s) 5, 521
MseI TTAA 3 cut(s) 29, 83, 552
MslI CAYNNNNRTG 2 cut(s) 158, 200
Msp20I TGGCCA 2 cut(s) 5, 521
MspA1I CMGCKG 2 cut(s) 71, 217
MspCI CTTAAG 1 cut(s) 82
MspI CCGG 2 cut(s) 312, 342
MwoI GCNNNNNNNGC 2 cut(s) 527, 536
NdeII GATC 2 cut(s) 408, 449
NheI GCTAGC 1 cut(s) 488
NlaIII CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
NmuCI GTSAC 2 cut(s) 160, 202
NsiI ATGCAT 1 cut(s) 291
NspV TTCGAA 1 cut(s) 426
OliI CACNNNNGTG 1 cut(s) 200
PagI TCATGA 1 cut(s) 153
PfeI GAWTC 1 cut(s) 602
PflMI CCANNNNNTGG 2 cut(s) 385, 517
PkrI GCNGC 4 cut(s) 26, 70, 73, 219
PleI GAGTC 3 cut(s) 157, 269, 323
PpsI GAGTC 3 cut(s) 157, 269, 323
Ppu21I YACGTR 1 cut(s) 648
PvuII CAGCTG 1 cut(s) 217
RsaI GTAC 2 cut(s) 646, 670
RsaNI GTAC 2 cut(s) 645, 669
RseI CAYNNNNRTG 2 cut(s) 158, 200
SaqAI TTAA 3 cut(s) 29, 83, 552
SatI GCNGC 4 cut(s) 25, 69, 72, 218
Sau3AI GATC 2 cut(s) 408, 449
SchI GAGTC 3 cut(s) 157, 270, 324
SduI GDGCHC 1 cut(s) 153
SetI ASST 5 cut(s) 29, 83, 219, 494, 650
SfuI TTCGAA 1 cut(s) 426
SmiMI CAYNNNNRTG 2 cut(s) 158, 200
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
SpeI ACTAGT 1 cut(s) 206
Sse9I AATT 2 cut(s) 88, 548
SsiI CCGC 2 cut(s) 69, 258
SspMI CTAG 5 cut(s) 207, 236, 332, 366, 489
TaaI ACNGT 3 cut(s) 406, 668, 677
TaiI ACGT 1 cut(s) 650
TaqI TCGA 3 cut(s) 76, 411, 426
TasI AATT 2 cut(s) 88, 548
TatI WGTACW 1 cut(s) 668
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 602
Tru1I TTAA 3 cut(s) 29, 83, 552
Tru9I TTAA 3 cut(s) 29, 83, 552
TscAI CASTG 2 cut(s) 459, 682
TseFI GTSAC 2 cut(s) 160, 202
TseI GCWGC 3 cut(s) 24, 71, 217
Tsp45I GTSAC 2 cut(s) 160, 202
TspDTI ATGAA 3 cut(s) 170, 569, 615
TspGWI ACGGA 1 cut(s) 573
TspRI CASTG 2 cut(s) 459, 682
Van91I CCANNNNNTGG 2 cut(s) 385, 517
Vha464I CTTAAG 1 cut(s) 82
XspI CTAG 5 cut(s) 207, 236, 332, 366, 489
Zsp2I ATGCAT 1 cut(s) 291
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.