RLG00000027427

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr6
OG0018075
Physical Location & Seq
Reverse (-)
11562690 .. 11563418
729 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000027427

Sequence Viewer

Length: 729 bp
ATGGCCATGCTAACCATAGCTTGCAGCTTAATCCTGCTAATCACCACCACCATAAACCACTCTTTTGCCGCTGCTCGAAGCTTAAGCAATTCAAACCCATCACACCCTCACAATCACCATCACAAACTCACATTCCTAATGAGAGATGTGCTCATGAATGTGACTCAACCCTCATCCAAGACCAAACCAGCAACCACCAAAGTGACTAGTCCTCAGCTGCCATTCTCAAAACCACTAGGACTATTTCCTCCAAATGGAGGAGTCCCCATCTCAGAAATCAATCCCATGCATACCACTACTTCTCCAGTTTCCGGAGTCTCCACTCAAACGCTAGATTTATCCAGCATTGGACTATTTTTTCCTGCTAGCGCCACACTGCAAGAACTGGAGTTTGGGGAAGTAACCGTGATCGATGAGGATATATTCGAAACTTTTACTTCAGGGTATGGTTCACTGGTCATTGGAAAAGCACAGGGGATATATGTTGCAAGCTCTGAAGATGGTAGTAGCCACATGATGGCCATGACTGCACATTTTGCTTATAGTAAATTTAAGGACGGATTGAGACTTTTCGGAGTTCATCGGAGGGATATGCATGATGAATCTCATGTTGCTGTTATTGGTGGCATTGGAAAGTATGTGGGTGCAAATGGCTATGCAACAGTTAAGACAGTGAAAACTGAAGAAGCAGCCAGCAAGATGCTTAAGCTCAATGTCTACCTCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

243

Amino Acids

26.04

Weight (kDa)

7.22

Isoelectric Point (pI)

43.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 125 - 241 8.7e-21 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018075)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20990
malus_domestica MD07G1189100.v1.1
prunus_persica Prupe.2G226600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365381
rosa_laevigata RLG00000027427
rosa_roxburghii Rroxscaffold_4G00291270
rosa_rugosa Rorug01G0321500
rosa_samantha Rh1AG330300 Rh1BG291900 Rh1DG323500
rosa_wichuraiana Rw1G029290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 517
AccI GTMKAC 1 cut(s) 717
AccIII TCCGGA 1 cut(s) 311
AciI CCGC 1 cut(s) 69
AcoI YGGCCR 2 cut(s) 3, 519
AcsI RAATTY 1 cut(s) 548
AcuI CTGAAG 3 cut(s) 423, 516, 702
AfiI CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
AflII CTTAAG 2 cut(s) 82, 704
AgsI TTSAA 1 cut(s) 93
AhlI ACTAGT 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 375, 407
AleI CACNNNNGTG 1 cut(s) 200
AluBI AGCT 7 cut(s) 20, 27, 81, 217, 492, 709, 726
AluI AGCT 7 cut(s) 20, 27, 81, 217, 492, 709, 726
Alw21I GWGCWC 1 cut(s) 153
Alw26I GTCTC 2 cut(s) 322, 559
Aor13HI TCCGGA 1 cut(s) 311
AoxI GGCC 2 cut(s) 3, 519
ApeKI GCWGC 4 cut(s) 24, 71, 217, 689
ApoI RAATTY 1 cut(s) 548
AspLEI GCGC 1 cut(s) 371
AsuHPI GGTGA 2 cut(s) 34, 107
AsuII TTCGAA 1 cut(s) 426
AsuNHI GCTAGC 1 cut(s) 365
BalI TGGCCA 2 cut(s) 5, 521
Bbv12I GWGCWC 1 cut(s) 153
BbvCI CCTCAGC 2 cut(s) 213, 722
BbvI GCAGC 4 cut(s) 36, 58, 204, 701
BccI CCATC 5 cut(s) 106, 126, 275, 494, 511
BcoDI GTCTC 2 cut(s) 322, 559
BcuI ACTAGT 1 cut(s) 206
BfaI CTAG 5 cut(s) 207, 236, 332, 366, 727
BfoI RGCGCY 1 cut(s) 372
BfrI CTTAAG 2 cut(s) 82, 704
BisI GCNGC 5 cut(s) 25, 69, 72, 218, 690
BlsI GCNGC 5 cut(s) 26, 70, 73, 219, 691
BmsI GCATC 1 cut(s) 690
BmtI GCTAGC 1 cut(s) 369
BplI GAGNNNNNCTC 2 cut(s) 135, 167
BpmI CTGGAG 2 cut(s) 288, 407
Bpu10I CCTNAGC 2 cut(s) 213, 722
Bpu14I TTCGAA 1 cut(s) 426
Bsa29I ATCGAT 1 cut(s) 411
BsaWI WCCGGW 1 cut(s) 311
BsaXI ACNNNNNCTCC 2 cut(s) 286, 316
Bsc4I CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
Bse1I ACTGG 3 cut(s) 305, 390, 459
BseAI TCCGGA 1 cut(s) 311
BseCI ATCGAT 1 cut(s) 411
BseGI GGATG 1 cut(s) 173
BseLI CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
BseMII CTCAG 2 cut(s) 227, 285
BseNI ACTGG 3 cut(s) 305, 390, 459
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 4 cut(s) 36, 58, 204, 701
BsgI GTGCAG 1 cut(s) 513
BshFI GGCC 2 cut(s) 5, 521
BshVI ATCGAT 1 cut(s) 411
BsiHKAI GWGCWC 1 cut(s) 153
BsiSI CCGG 1 cut(s) 312
BslFI GGGAC 1 cut(s) 248
BslI CCNNNNNNNGG 4 cut(s) 254, 257, 311, 517
BsmAI GTCTC 2 cut(s) 322, 559
BsmFI GGGAC 1 cut(s) 248
BsnI GGCC 2 cut(s) 5, 521
Bsp119I TTCGAA 1 cut(s) 426
Bsp1286I GDGCHC 1 cut(s) 153
Bsp13I TCCGGA 1 cut(s) 311
Bsp143I GATC 1 cut(s) 408
BspACI CCGC 1 cut(s) 69
BspANI GGCC 2 cut(s) 5, 521
BspCNI CTCAG 2 cut(s) 226, 284
BspDI ATCGAT 1 cut(s) 411
BspEI TCCGGA 1 cut(s) 311
BspHI TCATGA 1 cut(s) 153
BspOI GCTAGC 1 cut(s) 369
BspT104I TTCGAA 1 cut(s) 426
BspTI CTTAAG 2 cut(s) 82, 704
BsrI ACTGG 3 cut(s) 305, 390, 459
BssMI GATC 1 cut(s) 408
Bst4CI ACNGT 3 cut(s) 406, 664, 673
BstAFI CTTAAG 2 cut(s) 82, 704
BstAPI GCANNNNNTGC 1 cut(s) 536
BstBI TTCGAA 1 cut(s) 426
BstC8I GCNNGC 4 cut(s) 22, 367, 490, 694
BstDEI CTNAG 3 cut(s) 213, 271, 722
BstF5I GGATG 1 cut(s) 173
BstH2I RGCGCY 1 cut(s) 372
BstHHI GCGC 1 cut(s) 371
BstKTI GATC 1 cut(s) 411
BstMAI GTCTC 2 cut(s) 322, 559
BstMBI GATC 1 cut(s) 408
BstMWI GCNNNNNNNGC 2 cut(s) 527, 536
BstV1I GCAGC 4 cut(s) 36, 58, 204, 701
Bsu15I ATCGAT 1 cut(s) 411
BsuRI GGCC 2 cut(s) 5, 521
BsuTUI ATCGAT 1 cut(s) 411
BtsCI GGATG 1 cut(s) 173
BtsI GCAGTG 1 cut(s) 374
BtsIMutI CAGTG 3 cut(s) 374, 452, 678
Cac8I GCNNGC 4 cut(s) 22, 367, 490, 694
CciI TCATGA 1 cut(s) 153
CfoI GCGC 1 cut(s) 371
ClaI ATCGAT 1 cut(s) 411
CviAII CATG 7 cut(s) 7, 154, 286, 514, 523, 596, 608
DdeI CTNAG 3 cut(s) 213, 271, 722
DpnI GATC 1 cut(s) 410
DpnII GATC 1 cut(s) 408
EaeI YGGCCR 2 cut(s) 3, 519
Eco57I CTGAAG 3 cut(s) 423, 516, 702
EcoT22I ATGCAT 2 cut(s) 291, 597
FaeI CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
FaqI GGGAC 1 cut(s) 248
FatI CATG 7 cut(s) 6, 153, 285, 513, 522, 595, 607
FblI GTMKAC 1 cut(s) 717
Fnu4HI GCNGC 5 cut(s) 25, 69, 72, 218, 690
FokI GGATG 1 cut(s) 160
Fsp4HI GCNGC 5 cut(s) 25, 69, 72, 218, 690
FspBI CTAG 5 cut(s) 207, 236, 332, 366, 727
GlaI GCGC 1 cut(s) 370
GluI GCNGC 5 cut(s) 25, 69, 72, 218, 690
GsuI CTGGAG 2 cut(s) 288, 407
HaeII RGCGCY 1 cut(s) 372
HaeIII GGCC 2 cut(s) 5, 521
HapII CCGG 1 cut(s) 312
HhaI GCGC 1 cut(s) 371
Hin1II CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
Hin6I GCGC 1 cut(s) 369
HinP1I GCGC 1 cut(s) 369
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 4 cut(s) 163, 261, 315, 602
HpaII CCGG 1 cut(s) 312
HphI GGTGA 2 cut(s) 34, 107
Hpy166II GTNNAC 2 cut(s) 452, 718
Hpy188I TCNGA 4 cut(s) 274, 496, 575, 585
Hpy188III TCNNGA 2 cut(s) 154, 312
Hpy8I GTNNAC 2 cut(s) 452, 718
HpyCH4III ACNGT 3 cut(s) 406, 664, 673
HpyCH4V TGCA 8 cut(s) 24, 289, 379, 488, 530, 595, 647, 659
HpyF10VI GCNNNNNNNGC 2 cut(s) 527, 536
HpyF3I CTNAG 3 cut(s) 213, 271, 722
Hsp92II CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
HspAI GCGC 1 cut(s) 369
Kpn2I TCCGGA 1 cut(s) 311
Kzo9I GATC 1 cut(s) 408
Lsp1109I GCAGC 4 cut(s) 36, 58, 204, 701
LweI GCATC 1 cut(s) 690
MaeI CTAG 5 cut(s) 207, 236, 332, 366, 727
MaeIII GTNAC 3 cut(s) 160, 202, 400
MalI GATC 1 cut(s) 410
MboI GATC 1 cut(s) 408
MboII GAAGA 2 cut(s) 509, 695
MhlI GDGCHC 1 cut(s) 153
MlsI TGGCCA 2 cut(s) 5, 521
MluCI AATT 2 cut(s) 88, 548
MluNI TGGCCA 2 cut(s) 5, 521
MlyI GAGTC 3 cut(s) 157, 270, 324
MnlI CCTC 7 cut(s) 117, 181, 222, 251, 258, 409, 579
Mox20I TGGCCA 2 cut(s) 5, 521
Mph1103I ATGCAT 2 cut(s) 291, 597
MroI TCCGGA 1 cut(s) 311
MscI TGGCCA 2 cut(s) 5, 521
MseI TTAA 5 cut(s) 29, 83, 552, 666, 705
MslI CAYNNNNRTG 2 cut(s) 158, 200
Msp20I TGGCCA 2 cut(s) 5, 521
MspA1I CMGCKG 2 cut(s) 71, 217
MspCI CTTAAG 2 cut(s) 82, 704
MspI CCGG 1 cut(s) 312
MwoI GCNNNNNNNGC 2 cut(s) 527, 536
NdeII GATC 1 cut(s) 408
NheI GCTAGC 1 cut(s) 365
NlaIII CATG 7 cut(s) 10, 157, 289, 517, 526, 599, 611
NmuCI GTSAC 2 cut(s) 160, 202
NsiI ATGCAT 2 cut(s) 291, 597
NspV TTCGAA 1 cut(s) 426
OliI CACNNNNGTG 1 cut(s) 200
PagI TCATGA 1 cut(s) 153
PfeI GAWTC 1 cut(s) 602
PflMI CCANNNNNTGG 1 cut(s) 517
PkrI GCNGC 5 cut(s) 26, 70, 73, 219, 691
PleI GAGTC 3 cut(s) 157, 269, 323
PpsI GAGTC 3 cut(s) 157, 269, 323
PvuII CAGCTG 1 cut(s) 217
RseI CAYNNNNRTG 2 cut(s) 158, 200
SaqAI TTAA 5 cut(s) 29, 83, 552, 666, 705
SatI GCNGC 5 cut(s) 25, 69, 72, 218, 690
Sau3AI GATC 1 cut(s) 408
SchI GAGTC 3 cut(s) 157, 270, 324
SduI GDGCHC 1 cut(s) 153
SetI ASST 8 cut(s) 22, 29, 83, 219, 494, 711, 723, 728
SfaNI GCATC 1 cut(s) 690
SfuI TTCGAA 1 cut(s) 426
SmiMI CAYNNNNRTG 2 cut(s) 158, 200
SmlI CTYRAG 2 cut(s) 82, 704
SmoI CTYRAG 2 cut(s) 82, 704
SpeI ACTAGT 1 cut(s) 206
Sse9I AATT 2 cut(s) 88, 548
SsiI CCGC 1 cut(s) 69
SspMI CTAG 5 cut(s) 207, 236, 332, 366, 727
TaaI ACNGT 3 cut(s) 406, 664, 673
TaqI TCGA 3 cut(s) 76, 411, 426
TasI AATT 2 cut(s) 88, 548
TauI GCSGC 1 cut(s) 71
TfiI GAWTC 1 cut(s) 602
Tru1I TTAA 5 cut(s) 29, 83, 552, 666, 705
Tru9I TTAA 5 cut(s) 29, 83, 552, 666, 705
TscAI CASTG 3 cut(s) 381, 459, 678
TseFI GTSAC 2 cut(s) 160, 202
TseI GCWGC 4 cut(s) 24, 71, 217, 689
Tsp45I GTSAC 2 cut(s) 160, 202
TspDTI ATGAA 3 cut(s) 170, 569, 615
TspGWI ACGGA 1 cut(s) 573
TspRI CASTG 3 cut(s) 381, 459, 678
Van91I CCANNNNNTGG 1 cut(s) 517
Vha464I CTTAAG 2 cut(s) 82, 704
XapI RAATTY 1 cut(s) 548
XmiI GTMKAC 1 cut(s) 717
XspI CTAG 5 cut(s) 207, 236, 332, 366, 727
Zsp2I ATGCAT 2 cut(s) 291, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.