Rorug01G0321500

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000001
OG0018075
Physical Location & Seq
Forward (+)
44046614 .. 44047280
667 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug01G0321500.1

Sequence Viewer

Length: 426 bp
ATGACATGGAAGGACTATATCCGGTCTCACTTGATGTGCGATGTTACTGAGCTTCCCGGTCACGAAAATAACAGTGCCATGAAGGGCAATCACCTAACCGCAGCTGCAATCGTAGGACAAGATGGCACTGTTTGGGCTCAAAGCTCCTATTTCCCCTCGTTTGACAATGGTGAGATAACTGCGATTGTGGAAGAATTGGACCAGCCTGGAAGGATTGACATACTTATTGGTGGCAAGCAATACACATTAATTGAAGGAATAGACAACGAAGTCATTCGGGGAGAGCGGGGCTCTGGTGGAGTAACCATCAAAAGGACGAGGGAGGCATTGGTTATCGGCATCTATGAGGAGCCTGTGACTCATGACATGTGCAACGCACTTGTTGAAACATTAGGCGATTACATTGCTAATACTCTTGGAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

141

Amino Acids

15.44

Weight (kDa)

4.59

Isoelectric Point (pI)

31.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Profilin PF00235 1 - 137 9.5e-25 Profilin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018075)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20990
malus_domestica MD07G1189100.v1.1
prunus_persica Prupe.2G226600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365381
rosa_laevigata RLG00000027427
rosa_roxburghii Rroxscaffold_4G00291270
rosa_rugosa Rorug01G0321500
rosa_samantha Rh1AG330300 Rh1BG291900 Rh1DG323500
rosa_wichuraiana Rw1G029290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 269
AccBSI CCGCTC 1 cut(s) 286
AciI CCGC 2 cut(s) 99, 286
AfiI CCNNNNNNNGG 1 cut(s) 312
AflIII ACRYGT 1 cut(s) 366
AgsI TTSAA 2 cut(s) 254, 386
AjnI CCWGG 1 cut(s) 205
AluBI AGCT 3 cut(s) 52, 104, 144
AluI AGCT 3 cut(s) 52, 104, 144
Alw26I GTCTC 1 cut(s) 30
ApeKI GCWGC 2 cut(s) 101, 104
AseI ATTAAT 1 cut(s) 248
Asp700I GAANNNNTTC 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 199
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 2 cut(s) 83, 182
AvaII GGWCC 1 cut(s) 199
BanII GRGCYC 2 cut(s) 139, 293
BbvI GCAGC 2 cut(s) 91, 113
BccI CCATC 2 cut(s) 116, 314
BcgI CGANNNNNNTGC 2 cut(s) 386, 420
BciT130I CCWGG 1 cut(s) 207
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 1 cut(s) 30
BisI GCNGC 2 cut(s) 102, 105
BlsI GCNGC 2 cut(s) 103, 106
Bme1390I CCNGG 2 cut(s) 57, 207
Bme18I GGWCC 1 cut(s) 199
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 2 cut(s) 57, 207
BmsI GCATC 1 cut(s) 348
BplI GAGNNNNNCTC 2 cut(s) 275, 307
BpuMI CCSGG 1 cut(s) 57
BsaI GGTCTC 1 cut(s) 30
BsaWI WCCGGW 1 cut(s) 21
Bsc4I CCNNNNNNNGG 1 cut(s) 312
Bse3DI GCAATG 1 cut(s) 402
BseBI CCWGG 1 cut(s) 207
BseLI CCNNNNNNNGG 1 cut(s) 312
BseMI GCAATG 1 cut(s) 402
BseMII CTCAG 1 cut(s) 39
BseRI GAGGAG 1 cut(s) 362
BseXI GCAGC 2 cut(s) 91, 113
BsiSI CCGG 2 cut(s) 22, 57
BslI CCNNNNNNNGG 1 cut(s) 312
BsmAI GTCTC 1 cut(s) 30
Bso31I GGTCTC 1 cut(s) 30
Bsp1286I GDGCHC 2 cut(s) 139, 293
BspACI CCGC 2 cut(s) 99, 286
BspCNI CTCAG 1 cut(s) 40
BspHI TCATGA 1 cut(s) 361
BspLI GGNNCC 1 cut(s) 351
BspTNI GGTCTC 1 cut(s) 30
BsrBI CCGCTC 1 cut(s) 286
BsrDI GCAATG 1 cut(s) 402
Bst2UI CCWGG 1 cut(s) 207
Bst4CI ACNGT 2 cut(s) 74, 130
BstC8I GCNNGC 1 cut(s) 236
BstDEI CTNAG 1 cut(s) 48
BstMAI GTCTC 1 cut(s) 30
BstNI CCWGG 1 cut(s) 207
BstNSI RCATGY 1 cut(s) 370
BstSCI CCNGG 2 cut(s) 55, 205
BstV1I GCAGC 2 cut(s) 91, 113
BtgZI GCGATG 1 cut(s) 54
BtsIMutI CAGTG 2 cut(s) 79, 126
Cac8I GCNNGC 1 cut(s) 236
CciI TCATGA 1 cut(s) 361
Cfr13I GGNCC 1 cut(s) 199
CviAII CATG 4 cut(s) 6, 79, 362, 367
CviJI RGCY 7 cut(s) 52, 104, 137, 144, 205, 291, 352
CviKI_1 RGCY 7 cut(s) 52, 104, 137, 144, 205, 291, 352
DdeI CTNAG 1 cut(s) 48
DrdI GACNNNNNNGTC 1 cut(s) 269
DseDI GACNNNNNNGTC 1 cut(s) 269
Eco24I GRGCYC 2 cut(s) 139, 293
Eco31I GGTCTC 1 cut(s) 30
Eco47I GGWCC 1 cut(s) 199
EcoRII CCWGG 1 cut(s) 205
EcoT38I GRGCYC 2 cut(s) 139, 293
FaeI CATG 4 cut(s) 9, 82, 365, 370
FaiI YATR 7 cut(s) 7, 18, 80, 221, 345, 363, 368
FatI CATG 4 cut(s) 5, 78, 361, 366
FauI CCCGC 1 cut(s) 279
Fnu4HI GCNGC 2 cut(s) 102, 105
FriOI GRGCYC 2 cut(s) 139, 293
Fsp4HI GCNGC 2 cut(s) 102, 105
GluI GCNGC 2 cut(s) 102, 105
HapII CCGG 2 cut(s) 22, 57
Hin1II CATG 4 cut(s) 9, 82, 365, 370
HinfI GANTC 1 cut(s) 358
HpaII CCGG 2 cut(s) 22, 57
HphI GGTGA 2 cut(s) 83, 182
Hpy188III TCNNGA 2 cut(s) 62, 362
HpyAV CCTTC 4 cut(s) 4, 76, 204, 248
HpyCH4III ACNGT 2 cut(s) 74, 130
HpyCH4V TGCA 2 cut(s) 107, 372
HpyF3I CTNAG 1 cut(s) 48
Hsp92II CATG 4 cut(s) 9, 82, 365, 370
LmnI GCTCC 2 cut(s) 149, 349
LpnPI CCDG 7 cut(s) 35, 70, 192, 215, 219, 279, 366
Lsp1109I GCAGC 2 cut(s) 91, 113
LweI GCATC 1 cut(s) 348
MaeIII GTNAC 4 cut(s) 43, 59, 301, 355
MbiI CCGCTC 1 cut(s) 286
MboII GAAGA 1 cut(s) 203
MhlI GDGCHC 2 cut(s) 139, 293
MluCI AATT 2 cut(s) 194, 249
MlyI GAGTC 1 cut(s) 352
MnlI CCTC 4 cut(s) 166, 312, 316, 340
MroXI GAANNNNTTC 1 cut(s) 273
MseI TTAA 1 cut(s) 248
MspA1I CMGCKG 1 cut(s) 104
MspI CCGG 2 cut(s) 22, 57
MspR9I CCNGG 2 cut(s) 57, 207
MvaI CCWGG 1 cut(s) 207
NciI CCSGG 1 cut(s) 57
NlaIII CATG 4 cut(s) 9, 82, 365, 370
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 2 cut(s) 59, 355
NspI RCATGY 1 cut(s) 370
PagI TCATGA 1 cut(s) 361
PciI ACATGT 1 cut(s) 366
PdmI GAANNNNTTC 1 cut(s) 273
PkrI GCNGC 2 cut(s) 103, 106
PleI GAGTC 1 cut(s) 352
PpsI GAGTC 1 cut(s) 352
PscI ACATGT 1 cut(s) 366
PshBI ATTAAT 1 cut(s) 248
Psp6I CCWGG 1 cut(s) 205
PspGI CCWGG 1 cut(s) 205
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 1 cut(s) 199
PvuII CAGCTG 1 cut(s) 104
SaqAI TTAA 1 cut(s) 248
SatI GCNGC 2 cut(s) 102, 105
Sau96I GGNCC 1 cut(s) 199
SchI GAGTC 1 cut(s) 352
ScrFI CCNGG 2 cut(s) 57, 207
SduI GDGCHC 2 cut(s) 139, 293
SetI ASST 4 cut(s) 54, 96, 106, 146
SfaNI GCATC 1 cut(s) 348
SinI GGWCC 1 cut(s) 199
Sse9I AATT 2 cut(s) 194, 249
SsiI CCGC 2 cut(s) 99, 286
StyD4I CCNGG 2 cut(s) 55, 205
TaaI ACNGT 2 cut(s) 74, 130
TasI AATT 2 cut(s) 194, 249
Tru1I TTAA 1 cut(s) 248
Tru9I TTAA 1 cut(s) 248
TscAI CASTG 2 cut(s) 79, 133
TseFI GTSAC 2 cut(s) 59, 355
TseI GCWGC 2 cut(s) 101, 104
Tsp45I GTSAC 2 cut(s) 59, 355
TspDTI ATGAA 1 cut(s) 95
TspRI CASTG 2 cut(s) 79, 133
VpaK11BI GGWCC 1 cut(s) 199
VspI ATTAAT 1 cut(s) 248
XceI RCATGY 1 cut(s) 370
XmnI GAANNNNTTC 1 cut(s) 273
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.