MD07G1189100.v1.1

Dirigent proteins impart stereoselectivity on the phenoxy radical-coupling reaction, yielding optically active lignans from two molecules of coniferyl alcohol in the biosynthesis of lignans, flavonolignans, and alkaloids and thus plays a central role in plant secondary metabolism

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr07
OG0018075
Physical Location & Seq
Forward (+)
26957099 .. 26957818
720 bp
Loading structure...
UTR
Exon/CDS
Intron
MD07G1189100.v1.1.491

Sequence Viewer

Length: 720 bp
ATGGCCAAGCACCTTTCCTTCCTAGCCATGTCCTTATTAGTCCTGCTACTCACCACCGCCACGATCCACCCGTCCACCGCCGCTCGAAGCCTCGGCAGTTCAACCCCAACACACCCTCACAACCACCACAAAATCACTTTCCTAATGAGAAATGTTCTCAATGTAACCCACCCCTCATCATCCCTGTCCAAACCTTCAACCACTAAAGTGACTAGCCAGCTTCCCTTCTCAAAACCAATAGGCCTGCTTCCTCCCAACGGAGGAGTCCCCCTCCCTGAAACAATCCCCGCCACACAAACCCTAGATTTATCCGGTGTCGGACTATTTTTTCCTGCTAGGGCTACACTCCAAGAACTGGAACTTGGGATTGTAACCCTGATTGACGAGGACATCTTTGAAAGCTCAGGGTATTATGGTCCACAAATCATTGGAAAAGCACAAGGGATTTTTGTTGCAAGTTCTGAAGATGGGAGCAGTCACATGATGGCTATGACTGCACATTTCGCCGATGGCAGATTTAAGGACGGGTTAAGATTTTTTGGGGTGCACCGGACGGACGTGGATAAGCGGGACGGGTCTCATGTTGCTGTCATTGGTGGGATTGGGAAGTATGAGGGTGCAAATGGCTATGCAACTGTTAATGCAGTAAATAATGCAGGGGCTAATGCTATTTGGGAAGAAGAAAACAAGCTTCTTAGGCTCAATATTTACCTCAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Dirigent

Protein Analysis

240

Amino Acids

25.59

Weight (kDa)

6.83

Isoelectric Point (pI)

38.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Dirigent PF03018 117 - 226 4.3e-16 Dirigent-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0018075)

Species Orthologous Gene IDs
fragaria_vesca FvH4_7g20990
malus_domestica MD07G1189100.v1.1
prunus_persica Prupe.2G226600_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0365381
rosa_laevigata RLG00000027427
rosa_roxburghii Rroxscaffold_4G00291270
rosa_rugosa Rorug01G0321500
rosa_samantha Rh1AG330300 Rh1BG291900 Rh1DG323500
rosa_wichuraiana Rw1G029290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 355
AccBSI CCGCTC 1 cut(s) 83
AciI CCGC 5 cut(s) 57, 78, 81, 288, 568
AclWI GGATC 1 cut(s) 58
AcoI YGGCCR 1 cut(s) 3
AcuI CTGAAG 1 cut(s) 483
AfiI CCNNNNNNNGG 3 cut(s) 257, 260, 355
AgsI TTSAA 3 cut(s) 102, 198, 398
AjiI CACGTC 1 cut(s) 559
AjuI GAANNNNNNNTTGG 2 cut(s) 345, 377
AleI CACNNNNGTG 1 cut(s) 206
AluBI AGCT 4 cut(s) 220, 402, 691, 717
AluI AGCT 4 cut(s) 220, 402, 691, 717
Alw21I GWGCWC 1 cut(s) 549
Alw26I GTCTC 1 cut(s) 582
Alw44I GTGCAC 1 cut(s) 545
AlwI GGATC 1 cut(s) 58
AoxI GGCC 2 cut(s) 3, 241
ApaLI GTGCAC 1 cut(s) 545
AspS9I GGNCC 1 cut(s) 416
AsuHPI GGTGA 1 cut(s) 43
AvaII GGWCC 1 cut(s) 416
BaeGI GKGCMC 1 cut(s) 549
BalI TGGCCA 1 cut(s) 5
Bbv12I GWGCWC 1 cut(s) 549
BbvCI CCTCAGC 1 cut(s) 713
BccI CCATC 3 cut(s) 461, 478, 503
BcoDI GTCTC 1 cut(s) 582
BfaI CTAG 5 cut(s) 23, 213, 302, 336, 718
BisI GCNGC 1 cut(s) 81
BlsI GCNGC 1 cut(s) 82
Bme18I GGWCC 1 cut(s) 416
BmgBI CACGTC 1 cut(s) 559
BmgT120I GGNCC 1 cut(s) 416
BplI GAGNNNNNCTC 2 cut(s) 255, 287
Bpu10I CCTNAGC 2 cut(s) 403, 713
BsaI GGTCTC 1 cut(s) 582
BsaJI CCNNGG 1 cut(s) 91
BsaWI WCCGGW 2 cut(s) 311, 549
Bsc4I CCNNNNNNNGG 3 cut(s) 257, 260, 355
Bse1I ACTGG 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 91
BseGI GGATG 1 cut(s) 179
BseLI CCNNNNNNNGG 3 cut(s) 257, 260, 355
BseMII CTCAG 1 cut(s) 417
BseNI ACTGG 1 cut(s) 360
BseRI GAGGAG 1 cut(s) 276
BseSI GKGCMC 1 cut(s) 549
BsgI GTGCAG 1 cut(s) 480
BshFI GGCC 2 cut(s) 5, 243
BsiHKAI GWGCWC 1 cut(s) 549
BsiSI CCGG 2 cut(s) 312, 550
BslFI GGGAC 2 cut(s) 251, 584
BslI CCNNNNNNNGG 3 cut(s) 257, 260, 355
BsmAI GTCTC 1 cut(s) 582
BsmFI GGGAC 2 cut(s) 251, 584
BsnI GGCC 2 cut(s) 5, 243
Bso31I GGTCTC 1 cut(s) 582
Bsp1286I GDGCHC 1 cut(s) 549
Bsp143I GATC 1 cut(s) 63
BspACI CCGC 5 cut(s) 57, 78, 81, 288, 568
BspANI GGCC 2 cut(s) 5, 243
BspCNI CTCAG 1 cut(s) 416
BspPI GGATC 1 cut(s) 58
BspTNI GGTCTC 1 cut(s) 582
BsrBI CCGCTC 1 cut(s) 83
BsrI ACTGG 1 cut(s) 360
BssECI CCNNGG 1 cut(s) 91
BssMI GATC 1 cut(s) 63
Bst4CI ACNGT 1 cut(s) 637
BstC8I GCNNGC 2 cut(s) 218, 245
BstDEI CTNAG 3 cut(s) 403, 695, 713
BstF5I GGATG 1 cut(s) 179
BstKTI GATC 1 cut(s) 66
BstMAI GTCTC 1 cut(s) 582
BstMBI GATC 1 cut(s) 63
BstMWI GCNNNNNNNGC 3 cut(s) 494, 503, 697
BstSLI GKGCMC 1 cut(s) 549
BsuRI GGCC 2 cut(s) 5, 243
BtrI CACGTC 1 cut(s) 559
BtsCI GGATG 1 cut(s) 179
Cac8I GCNNGC 2 cut(s) 218, 245
Cfr13I GGNCC 1 cut(s) 416
CviAII CATG 3 cut(s) 28, 481, 581
DdeI CTNAG 3 cut(s) 403, 695, 713
DpnI GATC 1 cut(s) 65
DpnII GATC 1 cut(s) 63
EaeI YGGCCR 1 cut(s) 3
Eco147I AGGCCT 1 cut(s) 243
Eco31I GGTCTC 1 cut(s) 582
Eco47I GGWCC 1 cut(s) 416
Eco57I CTGAAG 1 cut(s) 483
FaeI CATG 3 cut(s) 31, 484, 584
FaiI YATR 7 cut(s) 29, 414, 482, 491, 582, 612, 630
FaqI GGGAC 2 cut(s) 251, 584
FatI CATG 3 cut(s) 27, 480, 580
FauI CCCGC 2 cut(s) 295, 561
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 1 cut(s) 166
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 5 cut(s) 23, 213, 302, 336, 718
GluI GCNGC 1 cut(s) 81
HaeIII GGCC 2 cut(s) 5, 243
HapII CCGG 2 cut(s) 312, 550
Hin1II CATG 3 cut(s) 31, 484, 584
HindIII AAGCTT 1 cut(s) 689
HinfI GANTC 1 cut(s) 264
HpaII CCGG 2 cut(s) 312, 550
HphI GGTGA 1 cut(s) 43
Hpy166II GTNNAC 3 cut(s) 75, 419, 547
Hpy188I TCNGA 2 cut(s) 320, 463
Hpy8I GTNNAC 3 cut(s) 75, 419, 547
HpyAV CCTTC 3 cut(s) 28, 204, 235
HpyCH4III ACNGT 1 cut(s) 637
HpyCH4IV ACGT 1 cut(s) 558
HpyCH4V TGCA 7 cut(s) 455, 497, 547, 620, 632, 644, 656
HpyF10VI GCNNNNNNNGC 3 cut(s) 494, 503, 697
HpyF3I CTNAG 3 cut(s) 403, 695, 713
HpySE526I ACGT 1 cut(s) 558
Hsp92II CATG 3 cut(s) 31, 484, 584
Kzo9I GATC 1 cut(s) 63
LmnI GCTCC 1 cut(s) 471
MaeI CTAG 5 cut(s) 23, 213, 302, 336, 718
MaeII ACGT 1 cut(s) 558
MaeIII GTNAC 4 cut(s) 163, 208, 370, 476
MalI GATC 1 cut(s) 65
MbiI CCGCTC 1 cut(s) 83
MboI GATC 1 cut(s) 63
MboII GAAGA 3 cut(s) 476, 689, 692
MhlI GDGCHC 1 cut(s) 549
MlsI TGGCCA 1 cut(s) 5
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 273
MmeI TCCRAC 1 cut(s) 298
MnlI CCTC 8 cut(s) 101, 126, 184, 254, 261, 281, 379, 607
Mox20I TGGCCA 1 cut(s) 5
MscI TGGCCA 1 cut(s) 5
MseI TTAA 3 cut(s) 519, 530, 639
MslI CAYNNNNRTG 1 cut(s) 206
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 2 cut(s) 312, 550
MwoI GCNNNNNNNGC 3 cut(s) 494, 503, 697
NdeII GATC 1 cut(s) 63
NlaIII CATG 3 cut(s) 31, 484, 584
NmeAIII GCCGAG 1 cut(s) 72
NmuCI GTSAC 2 cut(s) 208, 476
OliI CACNNNNGTG 1 cut(s) 206
PceI AGGCCT 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 68
PflMI CCANNNNNTGG 1 cut(s) 355
PkrI GCNGC 1 cut(s) 82
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
PspPI GGNCC 1 cut(s) 416
RseI CAYNNNNRTG 1 cut(s) 206
SaqAI TTAA 3 cut(s) 519, 530, 639
SatI GCNGC 1 cut(s) 81
Sau3AI GATC 1 cut(s) 63
Sau96I GGNCC 1 cut(s) 416
SchI GAGTC 1 cut(s) 273
SduI GDGCHC 1 cut(s) 549
SetI ASST 8 cut(s) 15, 196, 222, 404, 561, 693, 714, 719
SinI GGWCC 1 cut(s) 416
SmiMI CAYNNNNRTG 1 cut(s) 206
SseBI AGGCCT 1 cut(s) 243
SsiI CCGC 5 cut(s) 57, 78, 81, 288, 568
SspI AATATT 1 cut(s) 706
SspMI CTAG 5 cut(s) 23, 213, 302, 336, 718
StuI AGGCCT 1 cut(s) 243
TaaI ACNGT 1 cut(s) 637
TaiI ACGT 1 cut(s) 561
TaqI TCGA 1 cut(s) 85
TauI GCSGC 1 cut(s) 83
Tru1I TTAA 3 cut(s) 519, 530, 639
Tru9I TTAA 3 cut(s) 519, 530, 639
TseFI GTSAC 2 cut(s) 208, 476
Tsp45I GTSAC 2 cut(s) 208, 476
TspGWI ACGGA 2 cut(s) 273, 569
Van91I CCANNNNNTGG 1 cut(s) 355
VneI GTGCAC 1 cut(s) 545
VpaK11BI GGWCC 1 cut(s) 416
XspI CTAG 5 cut(s) 23, 213, 302, 336, 718
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.