FvH4_7g24670

Strictosidine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0013493
Physical Location & Seq
Reverse (-)
19028547 .. 19032504
3958 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g24670.t1

Sequence Viewer

Length: 1170 bp
ATGAAGCCAAATTTAAGGTTGTGTGCAGCAACAGTGGCCATAGTTATGAGTCTCCAGTTGTTCAAGTTGGGTCCAATCCCGGGTTCCCAAAACCTTCTCCACACCTCCGAAATACTCCAACTCACCGGAGCCGTCGGGCCGGAGAGTCTTGCTTTCGACCCCAACGGTGATGGCCCTTACACCGGTGTCGCCGATGGTCGTATCCTCAAGTGGGATCCCAACCACCACCGTTGGACTGATTTCGCAGTCACCACATCTCAAAGGAAGGAGTGTGTTAGGCCTTTTGCACCAGAATTGGAGCATGTTTGTGGAAGACCTTTAGGCCTAAGGTTCAATAAGAAAACTGGAGATCTCTATATTGCTGATGCATACCTTGGTTTACAAGTAGTAGGTCCTGCTGGAGGTTTAGCATCTCAAGTTGTTAAAGAAGTTGAAGGACAGCAGCTTCACTTCACCAATGATATTGACATTGATGAGAAGAAGGATGTGATTTATTTCACTGATTCGAGTACAGTGTTCCACAGAAGAAACAGTGCATCTCACTGGAAAATTATTGAGCTGTACAAGACAGCGGGTGCTTCCATGATCACTGTAATCTCAAAGCAATTCATGTCATCAATTTTGAATGGAGACAAGACTGGCAGATTACTGAAGTACAATATATCTAGCCAAGAAGTCGTGGTTTTACTACAAGGTCTTGCTTTTGCCAATGGTGTTGCGTTGAGCAAAGACCACTCCTTTGTGCTAGTAGCTGAAACCACTAATTGCAGGATCTTGAGGCATTGGCTTTATGGTCCAAATGCTGGAGATACAGATATATTTACCAAGCTTCCAGGATTCCCAGATAATATCCGAAGGAACTCGAAAGGTGAGTTTTGGGTTGCATTACATGCAAAGAAAGGACTTTTCGCGAAGTGGTTGGTGTCTAACACATGGGCTGGAAAGTCACTCCTAAAGCTTCCTCTAGGCTTCAAGCGATTGCATTCACTACTGGTAGGAGGAAAGGCTTATGCAACTGCTATAAAGTTAAGCGAGAAAGGTGATGTTTTGCAAGTATTGGAAGATTGTGAGGGAAAGAGCATGAGGTTTATCAGTGAAGTAGAAGAGAAGGATGGAAAACTTTGGATGGGGTCAGTTCTAATGCCTTTTATAGGGATATATAACTTGTAG

Protein Analysis

390

Amino Acids

43.0

Weight (kDa)

9.0

Isoelectric Point (pI)

35.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSL_N PF20067 33 - 71 3e-07 Strictosidine synthase-like, N-terminal
Str_synth PF03088 201 - 265 1.4e-20 Strictosidine synthase
SGL PF08450 210 - 301 2.9e-06 SMP-30/Gluconolactonase/LRE-like region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013493)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57030
fragaria_vesca FvH4_7g24670 FvH4_7g24670 FvH4_7g24670
malus_domestica MD14G1085600.v1.1
prunus_persica Prupe.7G041500_v2.0.a1 Prupe.7G041500_v2.0.a1
pyrus_communis pycom14g07530
rosa_chinensis RchiOBHm_Chr2g0125411
rosa_laevigata RLG00000018861
rosa_multiflora Rmu_sc0000878.1_g000007
rosa_roxburghii Rroxscaffold_2G00118050
rosa_rugosa Rorug02G0257100
rosa_samantha Rh2AG315700 Rh2BG323500 Rh2CG305700 Rh2DG342000
rosa_wichuraiana Rw2G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 803
AccII CGCG 1 cut(s) 911
AciI CCGC 1 cut(s) 572
AclWI GGATC 3 cut(s) 209, 222, 779
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 10
AcuI CTGAAG 1 cut(s) 671
AfaI GTAC 3 cut(s) 511, 563, 656
AfiI CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 803, 1151
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 5 cut(s) 64, 334, 434, 625, 973
AjnI CCWGG 1 cut(s) 832
AjuI GAANNNNNNNTTGG 2 cut(s) 67, 99
AluBI AGCT 5 cut(s) 445, 559, 752, 829, 958
AluI AGCT 5 cut(s) 445, 559, 752, 829, 958
Alw26I GTCTC 2 cut(s) 56, 624
AlwI GGATC 3 cut(s) 209, 222, 779
Ama87I CYCGRG 1 cut(s) 79
AoxI GGCC 5 cut(s) 36, 137, 172, 278, 322
ApeKI GCWGC 2 cut(s) 26, 442
ApoI RAATTY 1 cut(s) 10
ArsI GACNNNNNNTTYG 2 cut(s) 722, 754
AsiGI ACCGGT 1 cut(s) 182
AspS9I GGNCC 5 cut(s) 71, 137, 173, 392, 794
AsuC2I CCSGG 2 cut(s) 80, 81
AsuHPI GGTGA 6 cut(s) 115, 179, 241, 445, 881, 1052
AvaI CYCGRG 1 cut(s) 79
AvaII GGWCC 3 cut(s) 71, 392, 794
AxyI CCTNAGG 1 cut(s) 326
BalI TGGCCA 1 cut(s) 38
BamHI GGATCC 1 cut(s) 214
BbsI GAAGAC 1 cut(s) 319
BbvI GCAGC 2 cut(s) 38, 454
BccI CCATC 4 cut(s) 164, 188, 1106, 1120
BceAI ACGGC 1 cut(s) 116
BciT130I CCWGG 1 cut(s) 834
BciVI GTATCC 1 cut(s) 212
BclI TGATCA 1 cut(s) 585
BcnI CCSGG 2 cut(s) 80, 81
BcoDI GTCTC 2 cut(s) 56, 624
BfaI CTAG 3 cut(s) 666, 746, 965
BfuI GTATCC 1 cut(s) 212
BglII AGATCT 1 cut(s) 349
BisI GCNGC 2 cut(s) 27, 443
BlsI GCNGC 2 cut(s) 28, 444
Bme1390I CCNGG 3 cut(s) 80, 81, 834
Bme18I GGWCC 3 cut(s) 71, 392, 794
BmeT110I CYCGRG 1 cut(s) 79
BmgT120I GGNCC 5 cut(s) 71, 137, 173, 392, 794
BmiI GGNNCC 4 cut(s) 72, 85, 130, 216
BmrFI CCNGG 3 cut(s) 80, 81, 834
BmsI GCATC 3 cut(s) 355, 419, 545
BpiI GAAGAC 1 cut(s) 319
BpmI CTGGAG 4 cut(s) 38, 366, 420, 825
BpuEI CTTGAG 3 cut(s) 191, 399, 796
BpuMI CCSGG 2 cut(s) 80, 81
BsaJI CCNNGG 2 cut(s) 79, 373
BsaWI WCCGGW 2 cut(s) 125, 182
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 803, 1151
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 5 cut(s) 55, 349, 548, 643, 996
Bse21I CCTNAGG 1 cut(s) 326
BseBI CCWGG 1 cut(s) 834
BseDI CCNNGG 2 cut(s) 79, 373
BseGI GGATG 3 cut(s) 490, 1117, 1131
BseLI CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 803, 1151
BseNI ACTGG 5 cut(s) 55, 349, 548, 643, 996
BseXI GCAGC 2 cut(s) 38, 454
BsgI GTGCAG 1 cut(s) 45
Bsh1236I CGCG 1 cut(s) 911
BshFI GGCC 5 cut(s) 38, 139, 174, 280, 324
BshTI ACCGGT 1 cut(s) 182
BsiHKCI CYCGRG 1 cut(s) 79
BsiSI CCGG 4 cut(s) 80, 126, 140, 183
BslI CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 803, 1151
BsmAI GTCTC 2 cut(s) 56, 624
BsmI GAATGC 1 cut(s) 982
BsnI GGCC 5 cut(s) 38, 139, 174, 280, 324
BsoBI CYCGRG 1 cut(s) 79
Bsp1407I TGTACA 1 cut(s) 561
Bsp143I GATC 4 cut(s) 214, 349, 585, 771
Bsp68I TCGCGA 1 cut(s) 911
BspACI CCGC 1 cut(s) 572
BspANI GGCC 5 cut(s) 38, 139, 174, 280, 324
BspFNI CGCG 1 cut(s) 911
BspLI GGNNCC 4 cut(s) 72, 85, 130, 216
BspPI GGATC 3 cut(s) 209, 222, 779
BsrFI RCCGGY 1 cut(s) 182
BsrGI TGTACA 1 cut(s) 561
BsrI ACTGG 5 cut(s) 55, 349, 548, 643, 996
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 79, 373
BssMI GATC 4 cut(s) 214, 349, 585, 771
BssT1I CCWWGG 1 cut(s) 373
Bst2UI CCWGG 1 cut(s) 834
Bst4CI ACNGT 6 cut(s) 34, 167, 230, 514, 533, 592
Bst6I CTCTTC 1 cut(s) 1098
BstAPI GCANNNNNTGC 1 cut(s) 890
BstAUI TGTACA 1 cut(s) 561
BstDEI CTNAG 1 cut(s) 326
BstENI CCTNNNNNAGG 2 cut(s) 399, 1149
BstF5I GGATG 3 cut(s) 490, 1117, 1131
BstFNI CGCG 1 cut(s) 911
BstKTI GATC 4 cut(s) 217, 352, 588, 774
BstMAI GTCTC 2 cut(s) 56, 624
BstMBI GATC 4 cut(s) 214, 349, 585, 771
BstMWI GCNNNNNNNGC 2 cut(s) 35, 890
BstNI CCWGG 1 cut(s) 834
BstNSI RCATGY 2 cut(s) 305, 893
BstSCI CCNGG 3 cut(s) 78, 79, 832
BstUI CGCG 1 cut(s) 911
BstV1I GCAGC 2 cut(s) 38, 454
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 3 cut(s) 214, 349, 771
BstYI RGATCY 3 cut(s) 214, 349, 771
Bsu36I CCTNAGG 1 cut(s) 326
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 5 cut(s) 38, 139, 174, 280, 324
BtsCI GGATG 3 cut(s) 490, 1117, 1131
BtsIMutI CAGTG 7 cut(s) 39, 498, 519, 538, 541, 588, 1099
BtuMI TCGCGA 1 cut(s) 911
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 5 cut(s) 71, 137, 173, 392, 794
Cfr9I CCCGGG 1 cut(s) 79
Csp6I GTAC 3 cut(s) 510, 562, 655
CspAI ACCGGT 1 cut(s) 182
CspCI CAANNNNNGTGG 2 cut(s) 212, 247
CviAII CATG 6 cut(s) 302, 583, 610, 890, 933, 1081
CviQI GTAC 3 cut(s) 510, 562, 655
DdeI CTNAG 1 cut(s) 326
DpnI GATC 4 cut(s) 216, 351, 587, 773
DpnII GATC 4 cut(s) 214, 349, 585, 771
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 1098
EarI CTCTTC 1 cut(s) 1098
Eco130I CCWWGG 1 cut(s) 373
Eco147I AGGCCT 2 cut(s) 280, 324
Eco47I GGWCC 3 cut(s) 71, 392, 794
Eco57I CTGAAG 1 cut(s) 671
Eco81I CCTNAGG 1 cut(s) 326
Eco88I CYCGRG 1 cut(s) 79
EcoNI CCTNNNNNAGG 2 cut(s) 399, 1149
EcoO109I RGGNCCY 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 832
EcoT14I CCWWGG 1 cut(s) 373
EcoT22I ATGCAT 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 373
FaeI CATG 6 cut(s) 305, 586, 613, 893, 936, 1084
FatI CATG 6 cut(s) 301, 582, 609, 889, 932, 1080
FauI CCCGC 1 cut(s) 565
FbaI TGATCA 1 cut(s) 585
Fnu4HI GCNGC 2 cut(s) 27, 443
FokI GGATG 3 cut(s) 497, 1124, 1138
Fsp4HI GCNGC 2 cut(s) 27, 443
FspBI CTAG 3 cut(s) 666, 746, 965
GluI GCNGC 2 cut(s) 27, 443
GsuI CTGGAG 4 cut(s) 38, 366, 420, 825
HaeIII GGCC 5 cut(s) 38, 139, 174, 280, 324
HapII CCGG 4 cut(s) 80, 126, 140, 183
Hin1II CATG 6 cut(s) 305, 586, 613, 893, 936, 1084
HindIII AAGCTT 2 cut(s) 827, 956
HinfI GANTC 4 cut(s) 49, 145, 503, 837
HpaII CCGG 4 cut(s) 80, 126, 140, 183
HphI GGTGA 6 cut(s) 115, 179, 241, 445, 881, 1052
Hpy166II GTNNAC 1 cut(s) 380
Hpy188I TCNGA 2 cut(s) 109, 854
Hpy188III TCNNGA 2 cut(s) 775, 910
Hpy8I GTNNAC 1 cut(s) 380
Hpy99I CGWCG 1 cut(s) 137
HpyAV CCTTC 6 cut(s) 104, 259, 428, 475, 849, 1102
HpyCH4III ACNGT 6 cut(s) 34, 167, 230, 514, 533, 592
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 890
HpyF3I CTNAG 1 cut(s) 326
Hsp92II CATG 6 cut(s) 305, 586, 613, 893, 936, 1084
Ksp22I TGATCA 1 cut(s) 585
Kzo9I GATC 4 cut(s) 214, 349, 585, 771
LmnI GCTCC 2 cut(s) 128, 298
Lsp1109I GCAGC 2 cut(s) 38, 454
LweI GCATC 3 cut(s) 355, 419, 545
MaeI CTAG 3 cut(s) 666, 746, 965
MaeIII GTNAC 2 cut(s) 247, 945
MalI GATC 4 cut(s) 216, 351, 587, 773
MboI GATC 4 cut(s) 214, 349, 585, 771
MboII GAAGA 5 cut(s) 324, 490, 537, 1073, 1115
MflI RGATCY 3 cut(s) 214, 349, 771
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 6 cut(s) 10, 293, 549, 605, 618, 763
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 2 cut(s) 58, 154
MmeI TCCRAC 2 cut(s) 142, 212
MnlI CCTC 8 cut(s) 115, 215, 395, 771, 972, 992, 1063, 1077
Mox20I TGGCCA 1 cut(s) 38
Mph1103I ATGCAT 1 cut(s) 370
MscI TGGCCA 1 cut(s) 38
MseI TTAA 3 cut(s) 14, 423, 1028
MslI CAYNNNNRTG 2 cut(s) 44, 306
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 572
MspI CCGG 4 cut(s) 80, 126, 140, 183
MspR9I CCNGG 3 cut(s) 80, 81, 834
Mva1269I GAATGC 1 cut(s) 982
MvaI CCWGG 1 cut(s) 834
MvnI CGCG 1 cut(s) 911
MwoI GCNNNNNNNGC 2 cut(s) 35, 890
NciI CCSGG 2 cut(s) 80, 81
NdeII GATC 4 cut(s) 214, 349, 585, 771
NlaIII CATG 6 cut(s) 305, 586, 613, 893, 936, 1084
NlaIV GGNNCC 4 cut(s) 72, 85, 130, 216
NmuCI GTSAC 2 cut(s) 247, 945
NruI TCGCGA 1 cut(s) 911
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 2 cut(s) 305, 893
PceI AGGCCT 2 cut(s) 280, 324
PctI GAATGC 1 cut(s) 982
PfeI GAWTC 2 cut(s) 503, 837
PflMI CCANNNNNTGG 1 cut(s) 803
PfoI TCCNGGA 1 cut(s) 832
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 2 cut(s) 28, 444
PleI GAGTC 2 cut(s) 57, 153
PpsI GAGTC 2 cut(s) 57, 153
PpuMI RGGWCCY 1 cut(s) 392
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 832
PspGI CCWGG 1 cut(s) 832
PspN4I GGNNCC 4 cut(s) 72, 85, 130, 216
PspPI GGNCC 5 cut(s) 71, 137, 173, 392, 794
PspPPI RGGWCCY 1 cut(s) 392
PsuI RGATCY 3 cut(s) 214, 349, 771
RruI TCGCGA 1 cut(s) 911
RsaI GTAC 3 cut(s) 511, 563, 656
RsaNI GTAC 3 cut(s) 510, 562, 655
RseI CAYNNNNRTG 2 cut(s) 44, 306
SaqAI TTAA 3 cut(s) 14, 423, 1028
SatI GCNGC 2 cut(s) 27, 443
Sau3AI GATC 4 cut(s) 214, 349, 585, 771
Sau96I GGNCC 5 cut(s) 71, 137, 173, 392, 794
SchI GAGTC 2 cut(s) 58, 154
ScrFI CCNGG 3 cut(s) 80, 81, 834
SfaNI GCATC 3 cut(s) 355, 419, 545
SgrAI CRCCGGYG 1 cut(s) 182
SinI GGWCC 3 cut(s) 71, 392, 794
SmaI CCCGGG 1 cut(s) 81
SmiMI CAYNNNNRTG 2 cut(s) 44, 306
SmlI CTYRAG 3 cut(s) 206, 414, 775
SmoI CTYRAG 3 cut(s) 206, 414, 775
Sse9I AATT 6 cut(s) 10, 293, 549, 605, 618, 763
SseBI AGGCCT 2 cut(s) 280, 324
SsiI CCGC 1 cut(s) 572
SspMI CTAG 3 cut(s) 666, 746, 965
StuI AGGCCT 2 cut(s) 280, 324
StyD4I CCNGG 3 cut(s) 78, 79, 832
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 6 cut(s) 34, 167, 230, 514, 533, 592
TaqI TCGA 3 cut(s) 156, 506, 863
TasI AATT 6 cut(s) 10, 293, 549, 605, 618, 763
TatI WGTACW 3 cut(s) 509, 561, 654
TfiI GAWTC 2 cut(s) 503, 837
Tru1I TTAA 3 cut(s) 14, 423, 1028
Tru9I TTAA 3 cut(s) 14, 423, 1028
TscAI CASTG 7 cut(s) 39, 505, 519, 538, 548, 595, 1099
TseFI GTSAC 2 cut(s) 247, 945
TseI GCWGC 2 cut(s) 26, 442
Tsp45I GTSAC 2 cut(s) 247, 945
TspDTI ATGAA 2 cut(s) 17, 598
TspMI CCCGGG 1 cut(s) 79
TspRI CASTG 7 cut(s) 39, 505, 519, 538, 548, 595, 1099
Van91I CCANNNNNTGG 1 cut(s) 803
VpaK11BI GGWCC 3 cut(s) 71, 392, 794
XagI CCTNNNNNAGG 2 cut(s) 399, 1149
XapI RAATTY 1 cut(s) 10
XceI RCATGY 2 cut(s) 305, 893
XmaI CCCGGG 1 cut(s) 79
XspI CTAG 3 cut(s) 666, 746, 965
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.