Rroxscaffold_2G00118050

Strictosidine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0013493
Physical Location & Seq
Reverse (-)
49150236 .. 49153830
3595 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00118050.1

Sequence Viewer

Length: 1011 bp
ATGAAGCCAAATTTAACGTTGTGCGCAGCAGCAGTGGCCATAGTTTTGGGTCTCCAGTTATTCAAGTTGGGTCCAATCCCAGGTTCCCACGACCTTCTCCACACCTCCGAAATACTCCAAGTAACTGGAGCTGTCGGACCGGAGAGTCTTGCTTTCGACCCCAACGGTGATGGGCCTTACACCGGTGTCGCCGATGGTCGTATCCTCAAGTGGGATCAAAACGACCGCCGTTGGACTGATTTCGCAGTCACCACATCTCAAAGGAAGCAATGCGTTAGGCCTTTTGCACCAGAACTGGAGCATGTTTGTGGAAGACCTTTAGGACTAAGGTTCCATAAGCAAACTGGAGATCTCTATATTGCTGATGCATACCTTGGTTTACAAGTAGTAGGTCCTGCTGGAGGTTTAGCATCTCAAGTCGTTAAAGAAGTTGAAGGTCAGCGGCTTCGCTTCACCAATGATATGGACATTGATGAGCAGAAGGATGTAATTTACTTCACTGATTCGAGTACAATCTTCCACAGAAGGCAATTCATGTCGTCAATTTTGAATGGAGACAAGACTGGCAGATTACTAAGATACGATATATCTAGCCAAGAAGTTACGGATCTTGAGGCTTTGGGCTTTCAGGGTCCAAATGCTGGACATACAGATATTTTCACTGAGCTTCCGGGATTCCCAGATAATATCCGAAGGAACTCGAAAGGCGAGTTTTGGGTTGCACTACATGCAAAGAGGGGACTTTTCGCAAAGTGGTTGGTATCTAACACGTGGGCTGGAAAGTCACTCCTAAAGCTTCCCCCCCTAGATTTTAAGCGTCTGCATTCACTTCTAGTAGGAGGAAAGGCTCATGCAACTGCTATAAAGTTAAGCGAGAAAGGTGAGGTTTTGCAAGTTTTGGAAGATTGTGAGGGAAGGAGCATGAGGTTTATCAGTGAAATAGAAGAGAAGGATGGGAAACTCTGGATCGGGTCAGTTTTAATGCCTTTTCTAGGGATTTATAACCTGTAG

Protein Analysis

336

Amino Acids

37.24

Weight (kDa)

6.51

Isoelectric Point (pI)

39.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSL_N PF20067 33 - 71 9.5e-07 Strictosidine synthase-like, N-terminal
Str_synth PF03088 153 - 206 6.9e-16 Strictosidine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013493)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57030
fragaria_vesca FvH4_7g24670 FvH4_7g24670 FvH4_7g24670
malus_domestica MD14G1085600.v1.1
prunus_persica Prupe.7G041500_v2.0.a1 Prupe.7G041500_v2.0.a1
pyrus_communis pycom14g07530
rosa_chinensis RchiOBHm_Chr2g0125411
rosa_laevigata RLG00000018861
rosa_multiflora Rmu_sc0000878.1_g000007
rosa_roxburghii Rroxscaffold_2G00118050
rosa_rugosa Rorug02G0257100
rosa_samantha Rh2AG315700 Rh2BG323500 Rh2CG305700 Rh2DG342000
rosa_wichuraiana Rw2G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1002
AasI GACNNNNNNGTC 1 cut(s) 144
Acc16I TGCGCA 1 cut(s) 25
AccB7I CCANNNNNTGG 1 cut(s) 641
AciI CCGC 2 cut(s) 226, 442
AclI AACGTT 1 cut(s) 17
AclWI GGATC 3 cut(s) 222, 615, 974
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 10
AcvI CACGTG 1 cut(s) 771
AfaI GTAC 1 cut(s) 511
AfiI CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 641, 992
AflIII ACRYGT 1 cut(s) 768
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 3 cut(s) 64, 434, 550
AjnI CCWGG 1 cut(s) 79
AjuI GAANNNNNNNTTGG 2 cut(s) 67, 99
AluBI AGCT 3 cut(s) 131, 667, 796
AluI AGCT 3 cut(s) 131, 667, 796
Alw26I GTCTC 2 cut(s) 56, 549
AlwI GGATC 3 cut(s) 222, 615, 974
AoxI GGCC 3 cut(s) 36, 173, 278
ApeKI GCWGC 2 cut(s) 26, 29
ApoI RAATTY 1 cut(s) 10
AsiGI ACCGGT 1 cut(s) 182
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 5 cut(s) 71, 137, 173, 392, 632
AsuC2I CCSGG 1 cut(s) 672
AsuHPI GGTGA 4 cut(s) 179, 241, 445, 893
AvaII GGWCC 4 cut(s) 71, 137, 392, 632
BalI TGGCCA 1 cut(s) 38
BbrPI CACGTG 1 cut(s) 771
BbsI GAAGAC 1 cut(s) 319
BbvI GCAGC 2 cut(s) 38, 41
BccI CCATC 3 cut(s) 164, 188, 947
BceAI ACGGC 1 cut(s) 213
BciT130I CCWGG 1 cut(s) 81
BciVI GTATCC 1 cut(s) 212
BcnI CCSGG 1 cut(s) 672
BcoDI GTCTC 2 cut(s) 56, 549
BfaI CTAG 4 cut(s) 591, 806, 833, 992
BfmI CTRYAG 1 cut(s) 1007
BfuI GTATCC 1 cut(s) 212
BglII AGATCT 1 cut(s) 349
BisI GCNGC 3 cut(s) 27, 30, 443
BlsI GCNGC 3 cut(s) 28, 31, 444
Bme1390I CCNGG 2 cut(s) 81, 672
Bme18I GGWCC 4 cut(s) 71, 137, 392, 632
BmgT120I GGNCC 5 cut(s) 71, 137, 173, 392, 632
BmiI GGNNCC 4 cut(s) 72, 85, 332, 633
BmrFI CCNGG 2 cut(s) 81, 672
BmsI GCATC 2 cut(s) 355, 419
BpiI GAAGAC 1 cut(s) 319
BpmI CTGGAG 5 cut(s) 38, 147, 317, 366, 420
BpuEI CTTGAG 3 cut(s) 191, 399, 632
BpuMI CCSGG 1 cut(s) 672
BsaAI YACGTR 1 cut(s) 771
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 79, 373
BsaWI WCCGGW 2 cut(s) 139, 182
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 641, 992
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 5 cut(s) 55, 130, 300, 349, 568
Bse3DI GCAATG 1 cut(s) 275
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 79, 373
BseGI GGATG 2 cut(s) 490, 958
BseLI CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 641, 992
BseMI GCAATG 1 cut(s) 275
BseMII CTCAG 1 cut(s) 654
BseNI ACTGG 5 cut(s) 55, 130, 300, 349, 568
BseXI GCAGC 2 cut(s) 38, 41
Bsh1285I CGRYCG 1 cut(s) 226
BshFI GGCC 3 cut(s) 38, 175, 280
BshTI ACCGGT 1 cut(s) 182
BsiEI CGRYCG 1 cut(s) 226
BsiSI CCGG 3 cut(s) 140, 183, 671
BslFI GGGAC 1 cut(s) 753
BslI CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 641, 992
BsmAI GTCTC 2 cut(s) 56, 549
BsmFI GGGAC 1 cut(s) 753
BsmI GAATGC 1 cut(s) 823
BsnI GGCC 3 cut(s) 38, 175, 280
Bso31I GGTCTC 1 cut(s) 56
Bsp143I GATC 4 cut(s) 214, 349, 607, 966
BspACI CCGC 2 cut(s) 226, 442
BspANI GGCC 3 cut(s) 38, 175, 280
BspCNI CTCAG 1 cut(s) 655
BspLI GGNNCC 4 cut(s) 72, 85, 332, 633
BspPI GGATC 3 cut(s) 222, 615, 974
BspTNI GGTCTC 1 cut(s) 56
BsrDI GCAATG 1 cut(s) 275
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 5 cut(s) 55, 130, 300, 349, 568
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 79, 373
BssMI GATC 4 cut(s) 214, 349, 607, 966
BssT1I CCWWGG 1 cut(s) 373
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 1 cut(s) 167
Bst6I CTCTTC 1 cut(s) 939
BstAPI GCANNNNNTGC 1 cut(s) 728
BstBAI YACGTR 1 cut(s) 771
BstDEI CTNAG 3 cut(s) 326, 575, 663
BstENI CCTNNNNNAGG 2 cut(s) 399, 990
BstF5I GGATG 2 cut(s) 490, 958
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 4 cut(s) 217, 352, 610, 969
BstMAI GTCTC 2 cut(s) 56, 549
BstMBI GATC 4 cut(s) 214, 349, 607, 966
BstMCI CGRYCG 1 cut(s) 226
BstMWI GCNNNNNNNGC 2 cut(s) 35, 728
BstNI CCWGG 1 cut(s) 81
BstNSI RCATGY 2 cut(s) 305, 731
BstSCI CCNGG 2 cut(s) 79, 670
BstSFI CTRYAG 1 cut(s) 1007
BstV1I GCAGC 2 cut(s) 38, 41
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 2 cut(s) 349, 607
BstXI CCANNNNNNTGG 3 cut(s) 46, 125, 463
BstYI RGATCY 2 cut(s) 349, 607
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 3 cut(s) 38, 175, 280
BtsCI GGATG 2 cut(s) 490, 958
BtsI GCAGTG 1 cut(s) 39
BtsIMutI CAGTG 4 cut(s) 39, 498, 660, 940
CfoI GCGC 1 cut(s) 26
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 5 cut(s) 71, 137, 173, 392, 632
CpoI CGGWCCG 1 cut(s) 137
CseI GACGC 1 cut(s) 806
Csp6I GTAC 1 cut(s) 510
CspAI ACCGGT 1 cut(s) 182
CspI CGGWCCG 1 cut(s) 137
CviAII CATG 5 cut(s) 302, 535, 728, 851, 922
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 3 cut(s) 326, 575, 663
DpnI GATC 4 cut(s) 216, 351, 609, 968
DpnII GATC 4 cut(s) 214, 349, 607, 966
DrdI GACNNNNNNGTC 1 cut(s) 144
DseDI GACNNNNNNGTC 1 cut(s) 144
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 939
EarI CTCTTC 1 cut(s) 939
Eco130I CCWWGG 1 cut(s) 373
Eco147I AGGCCT 1 cut(s) 280
Eco31I GGTCTC 1 cut(s) 56
Eco47I GGWCC 4 cut(s) 71, 137, 392, 632
Eco72I CACGTG 1 cut(s) 771
EcoNI CCTNNNNNAGG 2 cut(s) 399, 990
EcoO109I RGGNCCY 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 373
EcoT22I ATGCAT 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 373
FaeI CATG 5 cut(s) 305, 538, 731, 854, 925
FaqI GGGAC 1 cut(s) 753
FatI CATG 5 cut(s) 301, 534, 727, 850, 921
Fnu4HI GCNGC 3 cut(s) 27, 30, 443
FokI GGATG 2 cut(s) 497, 965
Fsp4HI GCNGC 3 cut(s) 27, 30, 443
FspBI CTAG 4 cut(s) 591, 806, 833, 992
FspI TGCGCA 1 cut(s) 25
GlaI GCGC 1 cut(s) 25
GluI GCNGC 3 cut(s) 27, 30, 443
GsuI CTGGAG 5 cut(s) 38, 147, 317, 366, 420
HaeIII GGCC 3 cut(s) 38, 175, 280
HapII CCGG 3 cut(s) 140, 183, 671
HgaI GACGC 1 cut(s) 806
HhaI GCGC 1 cut(s) 26
Hin1II CATG 5 cut(s) 305, 538, 731, 854, 925
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HindIII AAGCTT 1 cut(s) 794
HinfI GANTC 3 cut(s) 145, 503, 675
HpaII CCGG 3 cut(s) 140, 183, 671
HphI GGTGA 4 cut(s) 179, 241, 445, 893
Hpy166II GTNNAC 1 cut(s) 380
Hpy188I TCNGA 3 cut(s) 109, 137, 692
Hpy188III TCNNGA 2 cut(s) 611, 964
Hpy8I GTNNAC 1 cut(s) 380
HpyAV CCTTC 7 cut(s) 104, 428, 475, 519, 687, 909, 943
HpyCH4III ACNGT 1 cut(s) 167
HpyCH4IV ACGT 2 cut(s) 17, 770
HpyCH4V TGCA 7 cut(s) 287, 368, 722, 731, 823, 854, 892
HpyF10VI GCNNNNNNNGC 2 cut(s) 35, 728
HpyF3I CTNAG 3 cut(s) 326, 575, 663
HpySE526I ACGT 2 cut(s) 17, 770
Hsp92II CATG 5 cut(s) 305, 538, 731, 854, 925
HspAI GCGC 1 cut(s) 24
Kzo9I GATC 4 cut(s) 214, 349, 607, 966
LmnI GCTCC 3 cut(s) 128, 298, 918
Lsp1109I GCAGC 2 cut(s) 38, 41
LweI GCATC 2 cut(s) 355, 419
MaeI CTAG 4 cut(s) 591, 806, 833, 992
MaeII ACGT 2 cut(s) 17, 770
MaeIII GTNAC 4 cut(s) 121, 247, 601, 783
MalI GATC 4 cut(s) 216, 351, 609, 968
MboI GATC 4 cut(s) 214, 349, 607, 966
MboII GAAGA 4 cut(s) 324, 508, 914, 956
MflI RGATCY 2 cut(s) 349, 607
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 4 cut(s) 10, 489, 530, 543
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 1 cut(s) 154
MmeI TCCRAC 2 cut(s) 115, 212
MnlI CCTC 9 cut(s) 115, 215, 395, 607, 729, 833, 877, 904, 918
Mox20I TGGCCA 1 cut(s) 38
Mph1103I ATGCAT 1 cut(s) 370
MscI TGGCCA 1 cut(s) 38
MseI TTAA 5 cut(s) 14, 423, 813, 869, 980
MslI CAYNNNNRTG 1 cut(s) 306
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 442
MspI CCGG 3 cut(s) 140, 183, 671
MspR9I CCNGG 2 cut(s) 81, 672
Mva1269I GAATGC 1 cut(s) 823
MvaI CCWGG 1 cut(s) 81
MwoI GCNNNNNNNGC 2 cut(s) 35, 728
NciI CCSGG 1 cut(s) 672
NdeII GATC 4 cut(s) 214, 349, 607, 966
NlaIII CATG 5 cut(s) 305, 538, 731, 854, 925
NlaIV GGNNCC 4 cut(s) 72, 85, 332, 633
NmuCI GTSAC 2 cut(s) 247, 783
NsbI TGCGCA 1 cut(s) 25
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 2 cut(s) 305, 731
PceI AGGCCT 1 cut(s) 280
PctI GAATGC 1 cut(s) 823
PfeI GAWTC 2 cut(s) 503, 675
PflMI CCANNNNNTGG 1 cut(s) 641
PfoI TCCNGGA 1 cut(s) 670
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 3 cut(s) 28, 31, 444
PleI GAGTC 1 cut(s) 153
PmaCI CACGTG 1 cut(s) 771
PmlI CACGTG 1 cut(s) 771
PpsI GAGTC 1 cut(s) 153
Ppu21I YACGTR 1 cut(s) 771
PpuMI RGGWCCY 1 cut(s) 392
PsiI TTATAA 1 cut(s) 1002
Psp1406I AACGTT 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 79
PspCI CACGTG 1 cut(s) 771
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 4 cut(s) 72, 85, 332, 633
PspPI GGNCC 5 cut(s) 71, 137, 173, 392, 632
PspPPI RGGWCCY 1 cut(s) 392
PsuI RGATCY 2 cut(s) 349, 607
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
RseI CAYNNNNRTG 1 cut(s) 306
Rsr2I CGGWCCG 1 cut(s) 137
RsrII CGGWCCG 1 cut(s) 137
SaqAI TTAA 5 cut(s) 14, 423, 813, 869, 980
SatI GCNGC 3 cut(s) 27, 30, 443
Sau3AI GATC 4 cut(s) 214, 349, 607, 966
Sau96I GGNCC 5 cut(s) 71, 137, 173, 392, 632
SchI GAGTC 1 cut(s) 154
ScrFI CCNGG 2 cut(s) 81, 672
SfaNI GCATC 2 cut(s) 355, 419
SfcI CTRYAG 1 cut(s) 1007
SgrAI CRCCGGYG 1 cut(s) 182
SinI GGWCC 4 cut(s) 71, 137, 392, 632
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 3 cut(s) 206, 414, 611
SmoI CTYRAG 3 cut(s) 206, 414, 611
Sse9I AATT 4 cut(s) 10, 489, 530, 543
SseBI AGGCCT 1 cut(s) 280
SsiI CCGC 2 cut(s) 226, 442
SspMI CTAG 4 cut(s) 591, 806, 833, 992
StuI AGGCCT 1 cut(s) 280
StyD4I CCNGG 2 cut(s) 79, 670
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 1 cut(s) 167
TaiI ACGT 2 cut(s) 20, 773
TaqI TCGA 3 cut(s) 156, 506, 701
TasI AATT 4 cut(s) 10, 489, 530, 543
TatI WGTACW 1 cut(s) 509
TauI GCSGC 1 cut(s) 445
TfiI GAWTC 2 cut(s) 503, 675
Tru1I TTAA 5 cut(s) 14, 423, 813, 869, 980
Tru9I TTAA 5 cut(s) 14, 423, 813, 869, 980
TscAI CASTG 4 cut(s) 39, 505, 667, 940
TseFI GTSAC 2 cut(s) 247, 783
TseI GCWGC 2 cut(s) 26, 29
Tsp45I GTSAC 2 cut(s) 247, 783
TspDTI ATGAA 2 cut(s) 17, 523
TspGWI ACGGA 1 cut(s) 620
TspRI CASTG 4 cut(s) 39, 505, 667, 940
Van91I CCANNNNNTGG 1 cut(s) 641
VpaK11BI GGWCC 4 cut(s) 71, 137, 392, 632
XagI CCTNNNNNAGG 2 cut(s) 399, 990
XapI RAATTY 1 cut(s) 10
XceI RCATGY 2 cut(s) 305, 731
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XspI CTAG 4 cut(s) 591, 806, 833, 992
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.