pycom14g07530

Strictosidine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr14
OG0013493
Physical Location & Seq
Reverse (-)
7684526 .. 7689481
4956 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom14g07530.1

Sequence Viewer

Length: 1128 bp
ATGAATCCAAAGTTAAGGTTGATTGCAGGGGTACTTGCGGTGGTGGCCGCAGCGTTGGCCGTCAACCCACCTGACTACTGGAAGCTCTTTGGGCTTGGAGTAGCTCCGATTCCGGGCTCCATTGACCTCCTCCACACTGCACAAGTCATCCCTGTTCCTGGAGCCTTGGGACCCGAGAGTCTCGCCTTCGACCCCACCGGAGATGGGCCCTACACCGGCGTCAACGACGGCCGTGTTCTCAAGTGGGATCGGAACAATCGCCGGTGGACTGATTTTGCAGTCACCACTTCCCAAAGGAAGGAATGTGTTCGCCCTTTCGCACCAGAAATGGAGCATGTCTGTGGAAGGCCTTTAGGATTAAAATTTGATAAGAAAAATGGAGATCTTTATATCGCTGATGCGTATCTTGGCCTTCAAGTAGTAGGTCCTGCTGGAGGGTTGGCATCTCAAGTGGTCAGAGAAGTTGAAGGTCAGCCACTTCGCTTCACCAATGACTTGGACATTGATGAGCATAAGGATGTAATTTACTTCACAGATTCAAGCACAATCTTCCAGAGAAGGCAGTTCATGTCATCAGTCTTGAACGGAGACAGGACTGGCAGATTACTAAAGTACAACAAATCAAGCAAAGAAGTAACTGTTTTACTACGAGGTCTTGCCTTTGCCAATGGTGTTGCTCTGAGCAAAGACCGCTCTTTTGTGCTAGTAGCTGAAACCACTACTTGCAGGATCTTGAGGATTTGGCTTGATGGTCCAAATGCTGGAAATACTGATGTTTTTGCTGAGCTTCCAGGATTCCCAGACAATGTAAGAAGGAACTCGAATGGAGAGTTTTGGGTTGCATTGCATGCAAAGAGTGGATTTGTATCCAAGTTTATTACCTCGAACACATGGGTTGGAAAGTCGATGTTAAAGCTTCCTCTCGGCTTTAAGCGGCTGCATTCCCTACTAGTAGGAGGCAAGGCCCATGCAACTGCCGTGAAGTTGAGCGAGAATGGAGAAGTTTTGCAAGTTTTGGAAGATTGTGAGGGAAAGACTATGAAGTTCATTAGTGAAGTGGAAGAGAAGGATGGGGAGCTCTGGATGGGGTCAGTTTTAATGCCTTTCATAGGCATTTATAAACTATAG

Protein Analysis

376

Amino Acids

41.18

Weight (kDa)

8.54

Isoelectric Point (pI)

30.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSL_N PF20067 43 - 82 1.7e-06 Strictosidine synthase-like, N-terminal
SGL PF08450 115 - 292 2.9e-12 SMP-30/Gluconolactonase/LRE-like region
Str_synth PF03088 164 - 251 1.1e-32 Strictosidine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013493)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57030
fragaria_vesca FvH4_7g24670 FvH4_7g24670 FvH4_7g24670
malus_domestica MD14G1085600.v1.1
prunus_persica Prupe.7G041500_v2.0.a1 Prupe.7G041500_v2.0.a1
pyrus_communis pycom14g07530
rosa_chinensis RchiOBHm_Chr2g0125411
rosa_laevigata RLG00000018861
rosa_multiflora Rmu_sc0000878.1_g000007
rosa_roxburghii Rroxscaffold_2G00118050
rosa_rugosa Rorug02G0257100
rosa_samantha Rh2AG315700 Rh2BG323500 Rh2CG305700 Rh2DG342000
rosa_wichuraiana Rw2G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1119
AasI GACNNNNNNGTC 1 cut(s) 177
AccB7I CCANNNNNTGG 1 cut(s) 761
AccBSI CCGCTC 1 cut(s) 693
AciI CCGC 4 cut(s) 38, 48, 691, 934
AclWI GGATC 2 cut(s) 255, 737
AcoI YGGCCR 3 cut(s) 45, 57, 229
AcsI RAATTY 1 cut(s) 362
AcyI GRCGYC 1 cut(s) 219
AfaI GTAC 2 cut(s) 33, 614
AfiI CCNNNNNNNGG 8 cut(s) 113, 158, 204, 215, 298, 434, 761, 1109
AgsI TTSAA 4 cut(s) 416, 467, 540, 583
AhlI ACTAGT 1 cut(s) 949
AjnI CCWGG 2 cut(s) 157, 790
AluBI AGCT 6 cut(s) 85, 104, 710, 787, 916, 1078
AluI AGCT 6 cut(s) 85, 104, 710, 787, 916, 1078
Alw21I GWGCWC 1 cut(s) 1080
Alw26I GTCTC 2 cut(s) 185, 582
AlwI GGATC 2 cut(s) 255, 737
Ama87I CYCGRG 1 cut(s) 173
AoxI GGCC 7 cut(s) 45, 57, 206, 229, 347, 409, 963
ApaI GGGCCC 1 cut(s) 210
ApeKI GCWGC 2 cut(s) 50, 937
ApoI RAATTY 1 cut(s) 362
ArsI GACNNNNNNTTYG 2 cut(s) 680, 712
Asp700I GAANNNNTTC 1 cut(s) 306
AspS9I GGNCC 6 cut(s) 170, 206, 207, 425, 752, 964
AsuC2I CCSGG 1 cut(s) 114
AsuHPI GGTGA 2 cut(s) 274, 478
AvaI CYCGRG 1 cut(s) 173
AvaII GGWCC 3 cut(s) 170, 425, 752
BaeGI GKGCMC 1 cut(s) 210
BanII GRGCYC 3 cut(s) 119, 210, 1080
Bbv12I GWGCWC 1 cut(s) 1080
BbvI GCAGC 2 cut(s) 62, 924
BccI CCATC 4 cut(s) 197, 743, 1064, 1078
BceAI ACGGC 4 cut(s) 44, 216, 244, 962
BciT130I CCWGG 2 cut(s) 159, 792
BciVI GTATCC 1 cut(s) 877
BcnI CCSGG 1 cut(s) 114
BcoDI GTCTC 2 cut(s) 185, 582
BcuI ACTAGT 1 cut(s) 949
BfaI CTAG 2 cut(s) 704, 950
BfmI CTRYAG 1 cut(s) 1124
BfuI GTATCC 1 cut(s) 877
BglII AGATCT 1 cut(s) 382
BisI GCNGC 4 cut(s) 48, 51, 935, 938
BlpI GCTNAGC 1 cut(s) 783
BlsI GCNGC 4 cut(s) 49, 52, 936, 939
Bme1390I CCNGG 3 cut(s) 114, 159, 792
Bme18I GGWCC 3 cut(s) 170, 425, 752
BmeT110I CYCGRG 1 cut(s) 173
BmgT120I GGNCC 6 cut(s) 170, 206, 207, 425, 752, 964
BmiI GGNNCC 5 cut(s) 118, 163, 171, 172, 208
BmrFI CCNGG 3 cut(s) 114, 159, 792
BmsI GCATC 2 cut(s) 388, 452
BpmI CTGGAG 2 cut(s) 180, 453
Bpu1102I GCTNAGC 1 cut(s) 783
BpuEI CTTGAG 3 cut(s) 224, 432, 754
BpuMI CCSGG 1 cut(s) 114
BsaBI GATNNNNATC 2 cut(s) 402, 865
BsaHI GRCGYC 1 cut(s) 219
BsaJI CCNNGG 1 cut(s) 165
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 8 cut(s) 113, 158, 204, 215, 298, 434, 761, 1109
Bse118I RCCGGY 2 cut(s) 215, 261
Bse1I ACTGG 2 cut(s) 83, 601
Bse3DI GCAATG 1 cut(s) 842
Bse8I GATNNNNATC 2 cut(s) 402, 865
BseBI CCWGG 2 cut(s) 159, 792
BseDI CCNNGG 1 cut(s) 165
BseGI GGATG 4 cut(s) 147, 523, 1075, 1089
BseJI GATNNNNATC 2 cut(s) 402, 865
BseLI CCNNNNNNNGG 8 cut(s) 113, 158, 204, 215, 298, 434, 761, 1109
BseMI GCAATG 1 cut(s) 842
BseMII CTCAG 2 cut(s) 671, 774
BseNI ACTGG 2 cut(s) 83, 601
BseRI GAGGAG 1 cut(s) 119
BseSI GKGCMC 1 cut(s) 210
BseX3I CGGCCG 1 cut(s) 229
BseXI GCAGC 2 cut(s) 62, 924
BsgI GTGCAG 1 cut(s) 123
Bsh1285I CGRYCG 1 cut(s) 232
BshFI GGCC 7 cut(s) 47, 59, 208, 231, 349, 411, 965
BsiEI CGRYCG 1 cut(s) 232
BsiHKAI GWGCWC 1 cut(s) 1080
BsiHKCI CYCGRG 1 cut(s) 173
BsiSI CCGG 4 cut(s) 113, 198, 216, 262
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 8 cut(s) 113, 158, 204, 215, 298, 434, 761, 1109
BsmAI GTCTC 2 cut(s) 185, 582
BsmFI GGGAC 1 cut(s) 183
BsmI GAATGC 1 cut(s) 940
BsnI GGCC 7 cut(s) 47, 59, 208, 231, 349, 411, 965
BsoBI CYCGRG 1 cut(s) 173
Bsp120I GGGCCC 1 cut(s) 206
Bsp1286I GDGCHC 3 cut(s) 119, 210, 1080
Bsp143I GATC 3 cut(s) 247, 382, 729
Bsp1720I GCTNAGC 1 cut(s) 783
BspACI CCGC 4 cut(s) 38, 48, 691, 934
BspANI GGCC 7 cut(s) 47, 59, 208, 231, 349, 411, 965
BspCNI CTCAG 2 cut(s) 672, 775
BspLI GGNNCC 5 cut(s) 118, 163, 171, 172, 208
BspPI GGATC 2 cut(s) 255, 737
BsrBI CCGCTC 1 cut(s) 693
BsrDI GCAATG 1 cut(s) 842
BsrFI RCCGGY 2 cut(s) 215, 261
BsrI ACTGG 2 cut(s) 83, 601
BssAI RCCGGY 2 cut(s) 215, 261
BssECI CCNNGG 1 cut(s) 165
BssMI GATC 3 cut(s) 247, 382, 729
BssNI GRCGYC 1 cut(s) 219
BssT1I CCWWGG 1 cut(s) 165
Bst2UI CCWGG 2 cut(s) 159, 792
Bst4CI ACNGT 1 cut(s) 640
Bst6I CTCTTC 1 cut(s) 1056
BstACI GRCGYC 1 cut(s) 219
BstAPI GCANNNNNTGC 1 cut(s) 848
BstC8I GCNNGC 1 cut(s) 849
BstDEI CTNAG 2 cut(s) 680, 783
BstENI CCTNNNNNAGG 2 cut(s) 432, 1107
BstF5I GGATG 4 cut(s) 147, 523, 1075, 1089
BstKTI GATC 3 cut(s) 250, 385, 732
BstMAI GTCTC 2 cut(s) 185, 582
BstMBI GATC 3 cut(s) 247, 382, 729
BstMCI CGRYCG 1 cut(s) 232
BstMWI GCNNNNNNNGC 5 cut(s) 44, 56, 91, 690, 848
BstNI CCWGG 2 cut(s) 159, 792
BstNSI RCATGY 2 cut(s) 338, 851
BstSCI CCNGG 3 cut(s) 112, 157, 790
BstSFI CTRYAG 1 cut(s) 1124
BstSLI GKGCMC 1 cut(s) 210
BstV1I GCAGC 2 cut(s) 62, 924
BstX2I RGATCY 2 cut(s) 382, 729
BstXI CCANNNNNNTGG 1 cut(s) 496
BstYI RGATCY 2 cut(s) 382, 729
BstZI CGGCCG 1 cut(s) 229
BsuI GTATCC 1 cut(s) 877
BsuRI GGCC 7 cut(s) 47, 59, 208, 231, 349, 411, 965
BtsCI GGATG 4 cut(s) 147, 523, 1075, 1089
BtsI GCAGTG 1 cut(s) 135
BtsIMutI CAGTG 1 cut(s) 135
Cac8I GCNNGC 1 cut(s) 849
Cfr10I RCCGGY 2 cut(s) 215, 261
Cfr13I GGNCC 6 cut(s) 170, 206, 207, 425, 752, 964
CseI GACGC 1 cut(s) 208
Csp6I GTAC 2 cut(s) 32, 613
CviAII CATG 5 cut(s) 335, 568, 848, 891, 968
CviQI GTAC 2 cut(s) 32, 613
DdeI CTNAG 2 cut(s) 680, 783
DpnI GATC 3 cut(s) 249, 384, 731
DpnII GATC 3 cut(s) 247, 382, 729
DrdI GACNNNNNNGTC 1 cut(s) 177
DseDI GACNNNNNNGTC 1 cut(s) 177
EaeI YGGCCR 3 cut(s) 45, 57, 229
EagI CGGCCG 1 cut(s) 229
Eam1104I CTCTTC 1 cut(s) 1056
EarI CTCTTC 1 cut(s) 1056
Ecl136II GAGCTC 1 cut(s) 1078
EclXI CGGCCG 1 cut(s) 229
Eco130I CCWWGG 1 cut(s) 165
Eco147I AGGCCT 1 cut(s) 349
Eco24I GRGCYC 3 cut(s) 119, 210, 1080
Eco47I GGWCC 3 cut(s) 170, 425, 752
Eco52I CGGCCG 1 cut(s) 229
Eco53kI GAGCTC 1 cut(s) 1078
Eco88I CYCGRG 1 cut(s) 173
EcoICRI GAGCTC 1 cut(s) 1078
EcoNI CCTNNNNNAGG 2 cut(s) 432, 1107
EcoO109I RGGNCCY 3 cut(s) 170, 207, 425
EcoRII CCWGG 2 cut(s) 157, 790
EcoT14I CCWWGG 1 cut(s) 165
EcoT38I GRGCYC 3 cut(s) 119, 210, 1080
ErhI CCWWGG 1 cut(s) 165
FaeI CATG 5 cut(s) 338, 571, 851, 894, 971
FaqI GGGAC 1 cut(s) 183
FatI CATG 5 cut(s) 334, 567, 847, 890, 967
Fnu4HI GCNGC 4 cut(s) 48, 51, 935, 938
FokI GGATG 4 cut(s) 134, 530, 1082, 1096
FriOI GRGCYC 3 cut(s) 119, 210, 1080
Fsp4HI GCNGC 4 cut(s) 48, 51, 935, 938
FspBI CTAG 2 cut(s) 704, 950
GluI GCNGC 4 cut(s) 48, 51, 935, 938
GsuI CTGGAG 2 cut(s) 180, 453
HaeIII GGCC 7 cut(s) 47, 59, 208, 231, 349, 411, 965
HapII CCGG 4 cut(s) 113, 198, 216, 262
HgaI GACGC 1 cut(s) 208
Hin1I GRCGYC 1 cut(s) 219
Hin1II CATG 5 cut(s) 338, 571, 851, 894, 971
HincII GTYRAC 2 cut(s) 64, 223
HindII GTYRAC 2 cut(s) 64, 223
HindIII AAGCTT 1 cut(s) 914
HinfI GANTC 5 cut(s) 4, 109, 178, 536, 795
HpaII CCGG 4 cut(s) 113, 198, 216, 262
HphI GGTGA 2 cut(s) 274, 478
Hpy166II GTNNAC 3 cut(s) 64, 223, 267
Hpy188I TCNGA 4 cut(s) 108, 252, 458, 681
Hpy188III TCNNGA 4 cut(s) 553, 580, 733, 1081
Hpy8I GTNNAC 3 cut(s) 64, 223, 267
Hpy99I CGWCG 1 cut(s) 230
HpyAV CCTTC 8 cut(s) 196, 292, 339, 422, 461, 552, 807, 1060
HpyCH4III ACNGT 1 cut(s) 640
HpyF10VI GCNNNNNNNGC 5 cut(s) 44, 56, 91, 690, 848
HpyF3I CTNAG 2 cut(s) 680, 783
Hsp92I GRCGYC 1 cut(s) 219
Hsp92II CATG 5 cut(s) 338, 571, 851, 894, 971
KflI GGGWCCC 1 cut(s) 170
Kzo9I GATC 3 cut(s) 247, 382, 729
LmnI GCTCC 5 cut(s) 109, 122, 161, 331, 1075
Lsp1109I GCAGC 2 cut(s) 62, 924
LweI GCATC 2 cut(s) 388, 452
MaeI CTAG 2 cut(s) 704, 950
MaeIII GTNAC 2 cut(s) 280, 634
MalI GATC 3 cut(s) 249, 384, 731
MbiI CCGCTC 1 cut(s) 693
MboI GATC 3 cut(s) 247, 382, 729
MboII GAAGA 3 cut(s) 541, 1031, 1073
MflI RGATCY 2 cut(s) 382, 729
MhlI GDGCHC 3 cut(s) 119, 210, 1080
MluCI AATT 2 cut(s) 362, 522
MlyI GAGTC 1 cut(s) 187
MmeI TCCRAC 1 cut(s) 877
MnlI CCTC 9 cut(s) 137, 140, 428, 644, 729, 892, 930, 950, 1021
MroXI GAANNNNTTC 1 cut(s) 306
MseI TTAA 5 cut(s) 14, 359, 911, 930, 1097
MslI CAYNNNNRTG 2 cut(s) 339, 516
MspI CCGG 4 cut(s) 113, 198, 216, 262
MspR9I CCNGG 3 cut(s) 114, 159, 792
Mva1269I GAATGC 1 cut(s) 940
MvaI CCWGG 2 cut(s) 159, 792
MwoI GCNNNNNNNGC 5 cut(s) 44, 56, 91, 690, 848
NciI CCSGG 1 cut(s) 114
NdeII GATC 3 cut(s) 247, 382, 729
NlaIII CATG 5 cut(s) 338, 571, 851, 894, 971
NlaIV GGNNCC 5 cut(s) 118, 163, 171, 172, 208
NmeAIII GCCGAG 1 cut(s) 903
NmuCI GTSAC 1 cut(s) 280
NspI RCATGY 2 cut(s) 338, 851
PaeI GCATGC 1 cut(s) 851
PceI AGGCCT 1 cut(s) 349
PctI GAATGC 1 cut(s) 940
PdmI GAANNNNTTC 1 cut(s) 306
PfeI GAWTC 4 cut(s) 4, 109, 536, 795
PflMI CCANNNNNTGG 1 cut(s) 761
PfoI TCCNGGA 2 cut(s) 157, 790
PkrI GCNGC 4 cut(s) 49, 52, 936, 939
PleI GAGTC 1 cut(s) 186
PpsI GAGTC 1 cut(s) 186
PpuMI RGGWCCY 2 cut(s) 170, 425
PsiI TTATAA 1 cut(s) 1119
Psp124BI GAGCTC 1 cut(s) 1080
Psp5II RGGWCCY 2 cut(s) 170, 425
Psp6I CCWGG 2 cut(s) 157, 790
PspGI CCWGG 2 cut(s) 157, 790
PspN4I GGNNCC 5 cut(s) 118, 163, 171, 172, 208
PspOMI GGGCCC 1 cut(s) 206
PspPI GGNCC 6 cut(s) 170, 206, 207, 425, 752, 964
PspPPI RGGWCCY 2 cut(s) 170, 425
PsuI RGATCY 2 cut(s) 382, 729
RsaI GTAC 2 cut(s) 33, 614
RsaNI GTAC 2 cut(s) 32, 613
RseI CAYNNNNRTG 2 cut(s) 339, 516
SacI GAGCTC 1 cut(s) 1080
SaqAI TTAA 5 cut(s) 14, 359, 911, 930, 1097
SatI GCNGC 4 cut(s) 48, 51, 935, 938
Sau3AI GATC 3 cut(s) 247, 382, 729
Sau96I GGNCC 6 cut(s) 170, 206, 207, 425, 752, 964
SchI GAGTC 1 cut(s) 187
ScrFI CCNGG 3 cut(s) 114, 159, 792
SduI GDGCHC 3 cut(s) 119, 210, 1080
SfaNI GCATC 2 cut(s) 388, 452
SfcI CTRYAG 1 cut(s) 1124
SgrAI CRCCGGYG 2 cut(s) 215, 261
SinI GGWCC 3 cut(s) 170, 425, 752
SmiMI CAYNNNNRTG 2 cut(s) 339, 516
SmlI CTYRAG 3 cut(s) 239, 447, 733
SmoI CTYRAG 3 cut(s) 239, 447, 733
SpeI ACTAGT 1 cut(s) 949
SphI GCATGC 1 cut(s) 851
Sse9I AATT 2 cut(s) 362, 522
SseBI AGGCCT 1 cut(s) 349
SsiI CCGC 4 cut(s) 38, 48, 691, 934
SspMI CTAG 2 cut(s) 704, 950
SstI GAGCTC 1 cut(s) 1080
StuI AGGCCT 1 cut(s) 349
StyD4I CCNGG 3 cut(s) 112, 157, 790
StyI CCWWGG 1 cut(s) 165
TaaI ACNGT 1 cut(s) 640
TaqI TCGA 4 cut(s) 189, 821, 884, 905
TasI AATT 2 cut(s) 362, 522
TatI WGTACW 1 cut(s) 612
TauI GCSGC 2 cut(s) 50, 937
TfiI GAWTC 4 cut(s) 4, 109, 536, 795
Tru1I TTAA 5 cut(s) 14, 359, 911, 930, 1097
Tru9I TTAA 5 cut(s) 14, 359, 911, 930, 1097
TscAI CASTG 1 cut(s) 142
TseFI GTSAC 1 cut(s) 280
TseI GCWGC 2 cut(s) 50, 937
Tsp45I GTSAC 1 cut(s) 280
TspDTI ATGAA 5 cut(s) 17, 556, 1036, 1055, 1096
TspGWI ACGGA 1 cut(s) 600
TspRI CASTG 1 cut(s) 142
Van91I CCANNNNNTGG 1 cut(s) 761
VpaK11BI GGWCC 3 cut(s) 170, 425, 752
XagI CCTNNNNNAGG 2 cut(s) 432, 1107
XapI RAATTY 1 cut(s) 362
XceI RCATGY 2 cut(s) 338, 851
XcmI CCANNNNNNNNNTGG 1 cut(s) 75
XmnI GAANNNNTTC 1 cut(s) 306
XspI CTAG 2 cut(s) 704, 950
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.