Rh2AG315700

Strictosidine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0013493
Physical Location & Seq
Forward (+)
41151182 .. 41155059
3878 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG315700.1

Sequence Viewer

Length: 1095 bp
ATGAAGCCAAATTTAACGTTGTGCGCAGCAACAGTGGCCATAGTTTTGGGTCTCCAGTTATTCAAGTTGGGTCCAATCCCAGGTTCCCACGACCTTCTCCACACCTCCGAAATACTCCAAGTGACTGGAGCCGTCGGACCGGAGAGTCTTGCTTTCGACCCCAACGGTGATGGGCCTTACACCGGTGTCGCCGACGGTCGTATCCTCAAGTGGGATCAAAACGACCGCCGTTGGACTGATTTCGCAGTCACCACATCTCAAAGGAAGGAATGTGTTAGGCCTTTTGCACCAGAACTGGAGCACGTTTGTGGAAGACCTTTAGGACTAAGGTTCCATAAGCAAACTGGAGATCTCTATATTGCTGATGCATACCTTGGTTTACAAGTAGTAGGTCCTGCTGGAGGTTTAGCATCTCAAGTGGTTAAAGAAGTTGAAGGTCAGCGGCTTCGCTTCACTAATGATATGGACATTGATGAGCAAAAGGATGTAATTTACTTCACTGATTCGAGTACAATCTTCCACAGAAGGCAATTCATGTCGTCAATTTTGAATGGAGACAAGACTGGCAGATTACTAAGATACAATATATCTAGCCAAGAAGTCACGGTTTTATTACGAGGTCTTGCTTTTGCCAATGGTGTTGCATTGAGCAAAGACCACTCTTTTGTGCTAGTTGCTGAAACCACTAATTGCAGGATCTTGAGGCTTTGGCTTCAGGGTCCAAATGCTGGATATACAGATATTTTCACTGAGCTTCCGGGATTCCCAGATAATATCCGAAGGAACTCGAAAGGCGAGTTTTGGGTTGCACTACATGCAAAGAGGGGACTTTTCGCAAAGTGGTTGGTATCTAACACGTGGGCTGGAAAGTCGCTCCTAAAGCTTCCCCTAGATTTTAAGCGACTGCATTCACTTCTAGTAGGAGGAAAGGCTCATGCAACTGCCATAAAGTTAAGCGAGAAAGGTGAGGTTTTGCAAGTTTTGGAAGATTGTGAGGGAAGGAGCATGAGGTTTATCAGTGAAGTAGAAGAGAAGAATGGGAAACTGTGGATTGGGTCAGTTTTAATGCCTTTTCTAGGGATTTATAACCTGTAG

Protein Analysis

364

Amino Acids

40.42

Weight (kDa)

8.51

Isoelectric Point (pI)

38.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SSL_N PF20067 33 - 71 1.1e-06 Strictosidine synthase-like, N-terminal
SGL PF08450 105 - 273 4.8e-10 SMP-30/Gluconolactonase/LRE-like region
Str_synth PF03088 153 - 240 7.6e-33 Strictosidine synthase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013493)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57030
fragaria_vesca FvH4_7g24670 FvH4_7g24670 FvH4_7g24670
malus_domestica MD14G1085600.v1.1
prunus_persica Prupe.7G041500_v2.0.a1 Prupe.7G041500_v2.0.a1
pyrus_communis pycom14g07530
rosa_chinensis RchiOBHm_Chr2g0125411
rosa_laevigata RLG00000018861
rosa_multiflora Rmu_sc0000878.1_g000007
rosa_roxburghii Rroxscaffold_2G00118050
rosa_rugosa Rorug02G0257100
rosa_samantha Rh2AG315700 Rh2BG323500 Rh2CG305700 Rh2DG342000
rosa_wichuraiana Rw2G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1086
AasI GACNNNNNNGTC 1 cut(s) 144
Acc16I TGCGCA 1 cut(s) 25
AccB7I CCANNNNNTGG 1 cut(s) 728
AciI CCGC 2 cut(s) 226, 442
AclI AACGTT 1 cut(s) 17
AclWI GGATC 2 cut(s) 222, 704
AcoI YGGCCR 1 cut(s) 36
AcsI RAATTY 1 cut(s) 10
AcuI CTGAAG 1 cut(s) 698
AcvI CACGTG 1 cut(s) 858
AfaI GTAC 1 cut(s) 511
AfiI CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 728, 1076
AflIII ACRYGT 1 cut(s) 855
AgeI ACCGGT 1 cut(s) 182
AgsI TTSAA 3 cut(s) 64, 434, 550
AjnI CCWGG 1 cut(s) 79
AjuI GAANNNNNNNTTGG 2 cut(s) 67, 99
AleI CACNNNNGTG 1 cut(s) 306
AluBI AGCT 2 cut(s) 754, 883
AluI AGCT 2 cut(s) 754, 883
Alw21I GWGCWC 1 cut(s) 303
Alw26I GTCTC 2 cut(s) 56, 549
AlwI GGATC 2 cut(s) 222, 704
AoxI GGCC 3 cut(s) 36, 173, 278
ApeKI GCWGC 1 cut(s) 26
ApoI RAATTY 1 cut(s) 10
ArsI GACNNNNNNTTYG 2 cut(s) 647, 679
AsiGI ACCGGT 1 cut(s) 182
AspLEI GCGC 1 cut(s) 26
AspS9I GGNCC 5 cut(s) 71, 137, 173, 392, 719
AsuC2I CCSGG 1 cut(s) 759
AsuHPI GGTGA 3 cut(s) 179, 241, 977
AvaII GGWCC 4 cut(s) 71, 137, 392, 719
BaeI ACNNNNGTAYC 2 cut(s) 184, 217
BalI TGGCCA 1 cut(s) 38
BbrPI CACGTG 1 cut(s) 858
BbsI GAAGAC 1 cut(s) 319
Bbv12I GWGCWC 1 cut(s) 303
BbvI GCAGC 1 cut(s) 38
BccI CCATC 1 cut(s) 164
BceAI ACGGC 2 cut(s) 116, 213
BciT130I CCWGG 1 cut(s) 81
BciVI GTATCC 1 cut(s) 212
BcnI CCSGG 1 cut(s) 759
BcoDI GTCTC 2 cut(s) 56, 549
BfaI CTAG 5 cut(s) 591, 671, 890, 917, 1076
BfmI CTRYAG 1 cut(s) 1091
BfuI GTATCC 1 cut(s) 212
BglII AGATCT 1 cut(s) 349
BisI GCNGC 2 cut(s) 27, 443
BlsI GCNGC 2 cut(s) 28, 444
Bme1390I CCNGG 2 cut(s) 81, 759
Bme18I GGWCC 4 cut(s) 71, 137, 392, 719
BmgT120I GGNCC 5 cut(s) 71, 137, 173, 392, 719
BmiI GGNNCC 5 cut(s) 72, 85, 130, 332, 720
BmrFI CCNGG 2 cut(s) 81, 759
BmsI GCATC 2 cut(s) 355, 419
BpiI GAAGAC 1 cut(s) 319
BpmI CTGGAG 5 cut(s) 38, 147, 317, 366, 420
BpuEI CTTGAG 3 cut(s) 191, 399, 721
BpuMI CCSGG 1 cut(s) 759
BsaAI YACGTR 1 cut(s) 858
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 2 cut(s) 79, 373
BsaWI WCCGGW 2 cut(s) 139, 182
Bsc4I CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 728, 1076
Bse118I RCCGGY 1 cut(s) 182
Bse1I ACTGG 5 cut(s) 55, 130, 300, 349, 568
BseBI CCWGG 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 79, 373
BseGI GGATG 1 cut(s) 490
BseLI CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 728, 1076
BseMII CTCAG 1 cut(s) 741
BseNI ACTGG 5 cut(s) 55, 130, 300, 349, 568
BseXI GCAGC 1 cut(s) 38
Bsh1285I CGRYCG 2 cut(s) 199, 226
BshFI GGCC 3 cut(s) 38, 175, 280
BshTI ACCGGT 1 cut(s) 182
BsiEI CGRYCG 2 cut(s) 199, 226
BsiHKAI GWGCWC 1 cut(s) 303
BsiSI CCGG 3 cut(s) 140, 183, 758
BslFI GGGAC 1 cut(s) 840
BslI CCNNNNNNNGG 6 cut(s) 80, 182, 211, 401, 728, 1076
BsmAI GTCTC 2 cut(s) 56, 549
BsmFI GGGAC 1 cut(s) 840
BsmI GAATGC 1 cut(s) 907
BsnI GGCC 3 cut(s) 38, 175, 280
Bso31I GGTCTC 1 cut(s) 56
Bsp1286I GDGCHC 1 cut(s) 303
Bsp143I GATC 3 cut(s) 214, 349, 696
BspACI CCGC 2 cut(s) 226, 442
BspANI GGCC 3 cut(s) 38, 175, 280
BspCNI CTCAG 1 cut(s) 742
BspLI GGNNCC 5 cut(s) 72, 85, 130, 332, 720
BspPI GGATC 2 cut(s) 222, 704
BspTNI GGTCTC 1 cut(s) 56
BsrFI RCCGGY 1 cut(s) 182
BsrI ACTGG 5 cut(s) 55, 130, 300, 349, 568
BssAI RCCGGY 1 cut(s) 182
BssECI CCNNGG 2 cut(s) 79, 373
BssMI GATC 3 cut(s) 214, 349, 696
BssT1I CCWWGG 1 cut(s) 373
Bst2UI CCWGG 1 cut(s) 81
Bst4CI ACNGT 5 cut(s) 34, 167, 197, 607, 1047
Bst6I CTCTTC 1 cut(s) 1023
BstAPI GCANNNNNTGC 1 cut(s) 815
BstBAI YACGTR 1 cut(s) 858
BstDEI CTNAG 3 cut(s) 326, 575, 750
BstENI CCTNNNNNAGG 2 cut(s) 399, 1074
BstF5I GGATG 1 cut(s) 490
BstHHI GCGC 1 cut(s) 26
BstKTI GATC 3 cut(s) 217, 352, 699
BstMAI GTCTC 2 cut(s) 56, 549
BstMBI GATC 3 cut(s) 214, 349, 696
BstMCI CGRYCG 2 cut(s) 199, 226
BstMWI GCNNNNNNNGC 3 cut(s) 35, 815, 880
BstNI CCWGG 1 cut(s) 81
BstNSI RCATGY 1 cut(s) 818
BstSCI CCNGG 2 cut(s) 79, 757
BstSFI CTRYAG 1 cut(s) 1091
BstV1I GCAGC 1 cut(s) 38
BstV2I GAAGAC 1 cut(s) 319
BstX2I RGATCY 2 cut(s) 349, 696
BstXI CCANNNNNNTGG 2 cut(s) 46, 125
BstYI RGATCY 2 cut(s) 349, 696
BsuI GTATCC 1 cut(s) 212
BsuRI GGCC 3 cut(s) 38, 175, 280
BtsCI GGATG 1 cut(s) 490
BtsIMutI CAGTG 4 cut(s) 39, 498, 747, 1024
CfoI GCGC 1 cut(s) 26
Cfr10I RCCGGY 1 cut(s) 182
Cfr13I GGNCC 5 cut(s) 71, 137, 173, 392, 719
CpoI CGGWCCG 1 cut(s) 137
Csp6I GTAC 1 cut(s) 510
CspAI ACCGGT 1 cut(s) 182
CspI CGGWCCG 1 cut(s) 137
CviAII CATG 4 cut(s) 535, 815, 935, 1006
CviQI GTAC 1 cut(s) 510
DdeI CTNAG 3 cut(s) 326, 575, 750
DpnI GATC 3 cut(s) 216, 351, 698
DpnII GATC 3 cut(s) 214, 349, 696
DrdI GACNNNNNNGTC 1 cut(s) 144
DseDI GACNNNNNNGTC 1 cut(s) 144
EaeI YGGCCR 1 cut(s) 36
Eam1104I CTCTTC 1 cut(s) 1023
EarI CTCTTC 1 cut(s) 1023
Eco130I CCWWGG 1 cut(s) 373
Eco147I AGGCCT 1 cut(s) 280
Eco31I GGTCTC 1 cut(s) 56
Eco47I GGWCC 4 cut(s) 71, 137, 392, 719
Eco57I CTGAAG 1 cut(s) 698
Eco72I CACGTG 1 cut(s) 858
EcoNI CCTNNNNNAGG 2 cut(s) 399, 1074
EcoO109I RGGNCCY 1 cut(s) 392
EcoRII CCWGG 1 cut(s) 79
EcoT14I CCWWGG 1 cut(s) 373
EcoT22I ATGCAT 1 cut(s) 370
ErhI CCWWGG 1 cut(s) 373
FaeI CATG 4 cut(s) 538, 818, 938, 1009
FaqI GGGAC 1 cut(s) 840
FatI CATG 4 cut(s) 534, 814, 934, 1005
Fnu4HI GCNGC 2 cut(s) 27, 443
FokI GGATG 1 cut(s) 497
Fsp4HI GCNGC 2 cut(s) 27, 443
FspBI CTAG 5 cut(s) 591, 671, 890, 917, 1076
FspI TGCGCA 1 cut(s) 25
GlaI GCGC 1 cut(s) 25
GluI GCNGC 2 cut(s) 27, 443
GsuI CTGGAG 5 cut(s) 38, 147, 317, 366, 420
HaeIII GGCC 3 cut(s) 38, 175, 280
HapII CCGG 3 cut(s) 140, 183, 758
HhaI GCGC 1 cut(s) 26
Hin1II CATG 4 cut(s) 538, 818, 938, 1009
Hin6I GCGC 1 cut(s) 24
HinP1I GCGC 1 cut(s) 24
HindIII AAGCTT 1 cut(s) 881
HinfI GANTC 3 cut(s) 145, 503, 762
HpaII CCGG 3 cut(s) 140, 183, 758
HphI GGTGA 3 cut(s) 179, 241, 977
Hpy166II GTNNAC 1 cut(s) 380
Hpy188I TCNGA 3 cut(s) 109, 137, 779
Hpy188III TCNNGA 1 cut(s) 700
Hpy8I GTNNAC 1 cut(s) 380
Hpy99I CGWCG 2 cut(s) 137, 197
HpyAV CCTTC 6 cut(s) 104, 259, 428, 519, 774, 993
HpyCH4III ACNGT 5 cut(s) 34, 167, 197, 607, 1047
HpyCH4IV ACGT 3 cut(s) 17, 303, 857
HpyCH4V TGCA 9 cut(s) 287, 368, 644, 693, 809, 818, 907, 938, 976
HpyF10VI GCNNNNNNNGC 3 cut(s) 35, 815, 880
HpyF3I CTNAG 3 cut(s) 326, 575, 750
HpySE526I ACGT 3 cut(s) 17, 303, 857
Hsp92II CATG 4 cut(s) 538, 818, 938, 1009
HspAI GCGC 1 cut(s) 24
Kzo9I GATC 3 cut(s) 214, 349, 696
LmnI GCTCC 4 cut(s) 128, 298, 879, 1002
Lsp1109I GCAGC 1 cut(s) 38
LweI GCATC 2 cut(s) 355, 419
MaeI CTAG 5 cut(s) 591, 671, 890, 917, 1076
MaeII ACGT 3 cut(s) 17, 303, 857
MaeIII GTNAC 3 cut(s) 121, 247, 601
MalI GATC 3 cut(s) 216, 351, 698
MboI GATC 3 cut(s) 214, 349, 696
MboII GAAGA 5 cut(s) 324, 508, 998, 1040, 1045
MflI RGATCY 2 cut(s) 349, 696
MhlI GDGCHC 1 cut(s) 303
MlsI TGGCCA 1 cut(s) 38
MluCI AATT 5 cut(s) 10, 489, 530, 543, 688
MluNI TGGCCA 1 cut(s) 38
MlyI GAGTC 1 cut(s) 154
MmeI TCCRAC 2 cut(s) 115, 212
Mox20I TGGCCA 1 cut(s) 38
Mph1103I ATGCAT 1 cut(s) 370
MscI TGGCCA 1 cut(s) 38
MseI TTAA 5 cut(s) 14, 423, 897, 953, 1064
MslI CAYNNNNRTG 1 cut(s) 306
Msp20I TGGCCA 1 cut(s) 38
MspA1I CMGCKG 1 cut(s) 442
MspI CCGG 3 cut(s) 140, 183, 758
MspR9I CCNGG 2 cut(s) 81, 759
Mva1269I GAATGC 1 cut(s) 907
MvaI CCWGG 1 cut(s) 81
MwoI GCNNNNNNNGC 3 cut(s) 35, 815, 880
NciI CCSGG 1 cut(s) 759
NdeII GATC 3 cut(s) 214, 349, 696
NlaIII CATG 4 cut(s) 538, 818, 938, 1009
NlaIV GGNNCC 5 cut(s) 72, 85, 130, 332, 720
NmuCI GTSAC 3 cut(s) 121, 247, 601
NsbI TGCGCA 1 cut(s) 25
NsiI ATGCAT 1 cut(s) 370
NspI RCATGY 1 cut(s) 818
OliI CACNNNNGTG 1 cut(s) 306
PceI AGGCCT 1 cut(s) 280
PctI GAATGC 1 cut(s) 907
PfeI GAWTC 2 cut(s) 503, 762
PflMI CCANNNNNTGG 1 cut(s) 728
PfoI TCCNGGA 1 cut(s) 757
PinAI ACCGGT 1 cut(s) 182
PkrI GCNGC 2 cut(s) 28, 444
PleI GAGTC 1 cut(s) 153
PmaCI CACGTG 1 cut(s) 858
PmlI CACGTG 1 cut(s) 858
PpsI GAGTC 1 cut(s) 153
Ppu21I YACGTR 1 cut(s) 858
PpuMI RGGWCCY 1 cut(s) 392
PsiI TTATAA 1 cut(s) 1086
Psp1406I AACGTT 1 cut(s) 17
Psp5II RGGWCCY 1 cut(s) 392
Psp6I CCWGG 1 cut(s) 79
PspCI CACGTG 1 cut(s) 858
PspGI CCWGG 1 cut(s) 79
PspN4I GGNNCC 5 cut(s) 72, 85, 130, 332, 720
PspPI GGNCC 5 cut(s) 71, 137, 173, 392, 719
PspPPI RGGWCCY 1 cut(s) 392
PsuI RGATCY 2 cut(s) 349, 696
RsaI GTAC 1 cut(s) 511
RsaNI GTAC 1 cut(s) 510
RseI CAYNNNNRTG 1 cut(s) 306
Rsr2I CGGWCCG 1 cut(s) 137
RsrII CGGWCCG 1 cut(s) 137
SaqAI TTAA 5 cut(s) 14, 423, 897, 953, 1064
SatI GCNGC 2 cut(s) 27, 443
Sau3AI GATC 3 cut(s) 214, 349, 696
Sau96I GGNCC 5 cut(s) 71, 137, 173, 392, 719
SchI GAGTC 1 cut(s) 154
ScrFI CCNGG 2 cut(s) 81, 759
SduI GDGCHC 1 cut(s) 303
SfaNI GCATC 2 cut(s) 355, 419
SfcI CTRYAG 1 cut(s) 1091
SgrAI CRCCGGYG 1 cut(s) 182
SinI GGWCC 4 cut(s) 71, 137, 392, 719
SmiMI CAYNNNNRTG 1 cut(s) 306
SmlI CTYRAG 3 cut(s) 206, 414, 700
SmoI CTYRAG 3 cut(s) 206, 414, 700
Sse9I AATT 5 cut(s) 10, 489, 530, 543, 688
SseBI AGGCCT 1 cut(s) 280
SsiI CCGC 2 cut(s) 226, 442
SspMI CTAG 5 cut(s) 591, 671, 890, 917, 1076
StuI AGGCCT 1 cut(s) 280
StyD4I CCNGG 2 cut(s) 79, 757
StyI CCWWGG 1 cut(s) 373
TaaI ACNGT 5 cut(s) 34, 167, 197, 607, 1047
TaiI ACGT 3 cut(s) 20, 306, 860
TaqI TCGA 3 cut(s) 156, 506, 788
TasI AATT 5 cut(s) 10, 489, 530, 543, 688
TatI WGTACW 1 cut(s) 509
TauI GCSGC 1 cut(s) 445
TfiI GAWTC 2 cut(s) 503, 762
Tru1I TTAA 5 cut(s) 14, 423, 897, 953, 1064
Tru9I TTAA 5 cut(s) 14, 423, 897, 953, 1064
TscAI CASTG 4 cut(s) 39, 505, 754, 1024
TseFI GTSAC 3 cut(s) 121, 247, 601
TseI GCWGC 1 cut(s) 26
Tsp45I GTSAC 3 cut(s) 121, 247, 601
TspDTI ATGAA 2 cut(s) 17, 523
TspRI CASTG 4 cut(s) 39, 505, 754, 1024
Van91I CCANNNNNTGG 1 cut(s) 728
VpaK11BI GGWCC 4 cut(s) 71, 137, 392, 719
XagI CCTNNNNNAGG 2 cut(s) 399, 1074
XapI RAATTY 1 cut(s) 10
XceI RCATGY 1 cut(s) 818
XcmI CCANNNNNNNNNTGG 1 cut(s) 341
XspI CTAG 5 cut(s) 591, 671, 890, 917, 1076
Zsp2I ATGCAT 1 cut(s) 370
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.