Rorug02G0257100

Strictosidine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0013493
Physical Location & Seq
Reverse (-)
26984043 .. 26987596
3554 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0257100.1

Sequence Viewer

Length: 612 bp
ATGTCATGTAGAGTCGACAAAGCTGATCTGGGGTGCGTCGTTTCATCGCTGTCGTCGTCGAGTCAGATTCTCTCTGATAAAAAAGCAGCACAGCACAGCTCAGCGCCTCAGCCTAACTCCAACCCATCATCCAAACTAAACCCAGACAAAGACGGCACCCCAGTAAATCAAGCTCTGCAATCTTGTTGTGAATCTGAGGACTTTATCTCATGGATGATCGAAAGGAGTGAGATAGAGGCTAATGCTATAGCTTCAGCGATAAGAATTATCTTGACATTTAAGAAAAATGCCCCATGGTTATCAGTGCCACAATTTGGGGACTGGGATCAGAAGGGCGGCCAAGTGCCAGACTACTCCCTTGATTTCTCCAAGATCAGGGAAATGAGGAAGCAAAACAAGAGAGATGTGTCCAGAGCAAGTCTTGGAAATGAAGAAGAGCTCATTGCCTCAAACACTGTCAATGTGGGTGCTGCCCACAATGACATTCACCACCCTCATTACGATCAGAGCACCCACTCCCCAACTAATAAGGATAAGATAGAGAAAACCATAGTATTGCTGCTGTTGCACAGGCTCGACGAAGCATATTCAGCTACTTCAACTGTTGCATAA

Protein Analysis

203

Amino Acids

22.35

Weight (kDa)

5.96

Isoelectric Point (pI)

58.44

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AvrRpt-cleavage PF05627 99 - 130 2.7e-08 Cleavage site for pathogenic type III effector avirulence factor Avr
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013493)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G57030
fragaria_vesca FvH4_7g24670 FvH4_7g24670 FvH4_7g24670
malus_domestica MD14G1085600.v1.1
prunus_persica Prupe.7G041500_v2.0.a1 Prupe.7G041500_v2.0.a1
pyrus_communis pycom14g07530
rosa_chinensis RchiOBHm_Chr2g0125411
rosa_laevigata RLG00000018861
rosa_multiflora Rmu_sc0000878.1_g000007
rosa_roxburghii Rroxscaffold_2G00118050
rosa_rugosa Rorug02G0257100
rosa_samantha Rh2AG315700 Rh2BG323500 Rh2CG305700 Rh2DG342000
rosa_wichuraiana Rw2G025480

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 155
AccB7I CCANNNNNTGG 1 cut(s) 314
AccI GTMKAC 1 cut(s) 15
AciI CCGC 1 cut(s) 336
AclWI GGATC 1 cut(s) 333
AcoI YGGCCR 1 cut(s) 337
AcuI CTGAAG 1 cut(s) 237
AfiI CCNNNNNNNGG 2 cut(s) 314, 375
AgsI TTSAA 1 cut(s) 600
AluBI AGCT 6 cut(s) 23, 99, 173, 251, 439, 593
AluI AGCT 6 cut(s) 23, 99, 173, 251, 439, 593
Alw21I GWGCWC 2 cut(s) 441, 512
AlwI GGATC 1 cut(s) 333
AoxI GGCC 1 cut(s) 337
ApeKI GCWGC 3 cut(s) 86, 470, 559
AspLEI GCGC 1 cut(s) 106
AsuHPI GGTGA 1 cut(s) 479
BanI GGYRCC 1 cut(s) 155
BanII GRGCYC 1 cut(s) 441
Bbv12I GWGCWC 2 cut(s) 441, 512
BbvCI CCTCAGC 1 cut(s) 108
BbvI GCAGC 3 cut(s) 98, 457, 546
BccI CCATC 1 cut(s) 133
BceAI ACGGC 1 cut(s) 169
BfmI CTRYAG 1 cut(s) 246
BfoI RGCGCY 1 cut(s) 107
BisI GCNGC 4 cut(s) 87, 337, 471, 560
BlpI GCTNAGC 1 cut(s) 100
BlsI GCNGC 4 cut(s) 88, 338, 472, 561
BmiI GGNNCC 1 cut(s) 157
BmrI ACTGGG 2 cut(s) 155, 331
BmuI ACTGGG 2 cut(s) 155, 331
Bpu10I CCTNAGC 1 cut(s) 108
Bpu1102I GCTNAGC 1 cut(s) 100
BsaJI CCNNGG 1 cut(s) 293
Bsc4I CCNNNNNNNGG 2 cut(s) 314, 375
Bse1I ACTGG 2 cut(s) 161, 326
Bse3DI GCAATG 1 cut(s) 441
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 2 cut(s) 128, 219
BseLI CCNNNNNNNGG 2 cut(s) 314, 375
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 3 cut(s) 114, 122, 186
BseNI ACTGG 2 cut(s) 161, 326
BseXI GCAGC 3 cut(s) 98, 457, 546
BshFI GGCC 1 cut(s) 339
BshNI GGYRCC 1 cut(s) 155
BsiHKAI GWGCWC 2 cut(s) 441, 512
BslFI GGGAC 1 cut(s) 332
BslI CCNNNNNNNGG 2 cut(s) 314, 375
BsmFI GGGAC 1 cut(s) 332
BsnI GGCC 1 cut(s) 339
Bsp1286I GDGCHC 2 cut(s) 441, 512
Bsp143I GATC 5 cut(s) 25, 216, 325, 372, 502
Bsp1720I GCTNAGC 1 cut(s) 100
Bsp19I CCATGG 1 cut(s) 293
BspACI CCGC 1 cut(s) 336
BspANI GGCC 1 cut(s) 339
BspCNI CTCAG 3 cut(s) 113, 121, 187
BspLI GGNNCC 1 cut(s) 157
BspPI GGATC 1 cut(s) 333
BspQI GCTCTTC 1 cut(s) 429
BspT107I GGYRCC 1 cut(s) 155
BsrDI GCAATG 1 cut(s) 441
BsrI ACTGG 2 cut(s) 161, 326
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 5 cut(s) 25, 216, 325, 372, 502
BssT1I CCWWGG 1 cut(s) 293
Bst4CI ACNGT 2 cut(s) 457, 604
Bst6I CTCTTC 1 cut(s) 429
BstDEI CTNAG 3 cut(s) 100, 108, 195
BstDSI CCRYGG 1 cut(s) 293
BstF5I GGATG 2 cut(s) 128, 219
BstH2I RGCGCY 1 cut(s) 107
BstHHI GCGC 1 cut(s) 106
BstKTI GATC 5 cut(s) 28, 219, 328, 375, 505
BstMBI GATC 5 cut(s) 25, 216, 325, 372, 502
BstMWI GCNNNNNNNGC 2 cut(s) 565, 590
BstSFI CTRYAG 1 cut(s) 246
BstV1I GCAGC 3 cut(s) 98, 457, 546
BsuRI GGCC 1 cut(s) 339
BtgI CCRYGG 1 cut(s) 293
BtgZI GCGATG 1 cut(s) 30
BtsCI GGATG 2 cut(s) 128, 219
BtsIMutI CAGTG 2 cut(s) 309, 453
CfoI GCGC 1 cut(s) 106
CseI GACGC 1 cut(s) 25
CviAII CATG 3 cut(s) 6, 210, 294
DdeI CTNAG 3 cut(s) 100, 108, 195
DpnI GATC 5 cut(s) 27, 218, 327, 374, 504
DpnII GATC 5 cut(s) 25, 216, 325, 372, 502
EaeI YGGCCR 1 cut(s) 337
Eam1104I CTCTTC 1 cut(s) 429
EarI CTCTTC 1 cut(s) 429
Ecl136II GAGCTC 1 cut(s) 439
Eco130I CCWWGG 1 cut(s) 293
Eco24I GRGCYC 1 cut(s) 441
Eco53kI GAGCTC 1 cut(s) 439
Eco57I CTGAAG 1 cut(s) 237
EcoICRI GAGCTC 1 cut(s) 439
EcoT14I CCWWGG 1 cut(s) 293
EcoT38I GRGCYC 1 cut(s) 441
ErhI CCWWGG 1 cut(s) 293
FaeI CATG 3 cut(s) 9, 213, 297
FaiI YATR 7 cut(s) 7, 211, 248, 295, 551, 586, 610
FaqI GGGAC 1 cut(s) 332
FatI CATG 3 cut(s) 5, 209, 293
FblI GTMKAC 1 cut(s) 15
Fnu4HI GCNGC 4 cut(s) 87, 337, 471, 560
FokI GGATG 2 cut(s) 115, 226
FriOI GRGCYC 1 cut(s) 441
Fsp4HI GCNGC 4 cut(s) 87, 337, 471, 560
GlaI GCGC 1 cut(s) 105
GluI GCNGC 4 cut(s) 87, 337, 471, 560
HaeII RGCGCY 1 cut(s) 107
HaeIII GGCC 1 cut(s) 339
HgaI GACGC 1 cut(s) 25
HhaI GCGC 1 cut(s) 106
Hin1II CATG 3 cut(s) 9, 213, 297
Hin6I GCGC 1 cut(s) 104
HinP1I GCGC 1 cut(s) 104
HincII GTYRAC 1 cut(s) 16
HindII GTYRAC 1 cut(s) 16
HinfI GANTC 4 cut(s) 12, 61, 67, 191
HphI GGTGA 1 cut(s) 479
Hpy166II GTNNAC 1 cut(s) 16
Hpy188I TCNGA 5 cut(s) 66, 76, 196, 330, 507
Hpy188III TCNNGA 2 cut(s) 271, 411
Hpy8I GTNNAC 1 cut(s) 16
Hpy99I CGWCG 4 cut(s) 41, 58, 61, 581
HpyAV CCTTC 1 cut(s) 325
HpyCH4III ACNGT 2 cut(s) 457, 604
HpyCH4V TGCA 3 cut(s) 178, 568, 608
HpyF10VI GCNNNNNNNGC 2 cut(s) 565, 590
HpyF3I CTNAG 3 cut(s) 100, 108, 195
Hsp92II CATG 3 cut(s) 9, 213, 297
HspAI GCGC 1 cut(s) 104
Kzo9I GATC 5 cut(s) 25, 216, 325, 372, 502
LguI GCTCTTC 1 cut(s) 429
LpnPI CCDG 8 cut(s) 14, 156, 174, 307, 360, 361, 424, 556
Lsp1109I GCAGC 3 cut(s) 98, 457, 546
MalI GATC 5 cut(s) 27, 218, 327, 374, 504
MboI GATC 5 cut(s) 25, 216, 325, 372, 502
MboII GAAGA 2 cut(s) 443, 446
MhlI GDGCHC 2 cut(s) 441, 512
MluCI AATT 2 cut(s) 264, 311
MlyI GAGTC 2 cut(s) 21, 70
MmeI TCCRAC 1 cut(s) 144
MnlI CCTC 6 cut(s) 117, 190, 229, 378, 457, 504
MseI TTAA 1 cut(s) 279
MwoI GCNNNNNNNGC 2 cut(s) 565, 590
NcoI CCATGG 1 cut(s) 293
NdeII GATC 5 cut(s) 25, 216, 325, 372, 502
NlaIII CATG 3 cut(s) 9, 213, 297
NlaIV GGNNCC 1 cut(s) 157
PciSI GCTCTTC 1 cut(s) 429
PcsI WCGNNNNNNNCGW 1 cut(s) 53
PfeI GAWTC 2 cut(s) 67, 191
PflMI CCANNNNNTGG 1 cut(s) 314
PkrI GCNGC 4 cut(s) 88, 338, 472, 561
PleI GAGTC 2 cut(s) 20, 69
PpsI GAGTC 2 cut(s) 20, 69
Psp124BI GAGCTC 1 cut(s) 441
PspN4I GGNNCC 1 cut(s) 157
SacI GAGCTC 1 cut(s) 441
SalI GTCGAC 1 cut(s) 14
SapI GCTCTTC 1 cut(s) 429
SaqAI TTAA 1 cut(s) 279
SatI GCNGC 4 cut(s) 87, 337, 471, 560
Sau3AI GATC 5 cut(s) 25, 216, 325, 372, 502
SchI GAGTC 2 cut(s) 21, 70
SduI GDGCHC 2 cut(s) 441, 512
SetI ASST 6 cut(s) 25, 101, 175, 253, 441, 595
SfcI CTRYAG 1 cut(s) 246
Sse9I AATT 2 cut(s) 264, 311
SsiI CCGC 1 cut(s) 336
SstI GAGCTC 1 cut(s) 441
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 2 cut(s) 457, 604
TaqI TCGA 4 cut(s) 15, 59, 219, 576
TasI AATT 2 cut(s) 264, 311
TauI GCSGC 1 cut(s) 339
TfiI GAWTC 2 cut(s) 67, 191
Tru1I TTAA 1 cut(s) 279
Tru9I TTAA 1 cut(s) 279
TscAI CASTG 2 cut(s) 309, 460
TseI GCWGC 3 cut(s) 86, 470, 559
TspDTI ATGAA 2 cut(s) 33, 444
TspRI CASTG 2 cut(s) 309, 460
Van91I CCANNNNNTGG 1 cut(s) 314
XmiI GTMKAC 1 cut(s) 15
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.