Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0014286

Physical Location & Seq

Genomic Coordinates Reverse (-)

1065060 .. 1067318

Gene Structure 2259 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1008100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2160 bp

ATGGCTTCGGCGGCGATATTCTCATCGATGCGGAGGCGGAGGTCACCTTCTCTGGAGGCATTTTTAGCTCCGGTTGACCTTTCCGACGTCGCCCTTGTACAGACCCTTGTCTCCGTCGCTGCCGACATAGTTTCCTCCTTCTCAGACAAGTTCTTATCCATCCAGCGGCGGAATTCGAGGTCCCTGGTGCGGAAAGTTGAGATCTTTCTCGTGCTCTTGGAGTATCTGAGGGACTCCGACGCTGCCCTGCCGTGTACGGCGGTCATCTGCCTGAAAGAGCTATACTTGCTGTTGTACAGATCCAAAATACTCCTCGATTATTGCGCACAATCCAGTAAGTTGTGGCTTTTGCTTCAGAACCATCCAATTTCCGGCCATTTTCACGATATGAATCAGGAAATTTCGACCCTTTTGGACGTTTTTCCGATGGAGGACGTCGGATTGGGGGAGGACGTGAGGGAGCAGATTGAGCTCCTGCAGCTACAGGCCAGGAGGGCGAGGCTGTTCATCGACGAAGGAGACGAGGAGCTGAGGGTTCAGTTCTTTTCGTTCCTTGACGAATTCGAGAAGGGCCGGGTTCCTGACTCGGCCGATTTGTGGTTGTTTTTGGTTGAAAGATTGGGGATTCGAGACGCCAAGAGTTGTAGGCACGAAATCGAGTTCTTGGAGGAGCAGATTGTGAACCACGAAGGTGACGTTGAGCCCACCGCTTCTGTGCTCAATGGGTTTGTGGCCTTCACTCGGTATTGCAGGTTTTTGCTTTTCGGTTTTGAAGAAGACGAGGTTGAATCGGGGATTGGGAACAATCAGAGGAAGCCCCGAAGACGAAGAGGTTTGCTTACTCAGGAGATTGCCGAGACTTTTATAACGATTCCGAAGGACTTTTGCTGCCCCATATCATTGGATTTGATGAGGGAGCCGGTGATCATTTCGACAGGACAGACGTATGATCGGAGTTCGATTTCGAGGTGGTGGGAAGAAGGGCATTGTACTTGCCCGAAAACAGGACAAATGCTGCCCAGCACTCGCCTTGTTCCGAATCGGGCTTTGAGGAATTTGATCATGCAGTGGTGCACTGCTTATGGAATTCCGTATGACCCTCCAGAGTTTGCAGATGCATCAGCAGACAGCTTTGCGGCTGTTTCTCCCTCTAAGGCTGCACTTGCAGCCAATAAAGCGACCGCGGGGCTTCTAATCCAACGGCTGGAGGATGGATCACCATGTGCACAAACTATAGCTGCTCGCGAACTTCGGTTGCTGGCAAAAACAGGAAGAGAAAACCGTGCTTTCATTGCGGAAGCCGGGGCAATTCCCCACCTTTGCAAGTTACTCTCGTCTCCAAACTCTGTTACCCAAGAGAATTCTGTAACTGCAATGCTCAACTTATCGATATATGAAAAAAACAAAAGCCGAATTGTGGACGAAGAAGGGTGTTTGGGGTCCATTGTTAATGTTCTGAGGTTTGGTCACACGACTGAGGCGAGGGAGAATGCTGCAGCAACTTTATTTAGTCTCTCTGCAGTTCATGACTATAAGAAGAGAATAGCAGACGAGGAAGGGGGAATCGAAGCTTTGGCAGAACTGTTGAGAGAGGGTACCCCAAGAGGAAAGAAGGACGCCGTGACAGCTTTGTTTAACCTGTCGACTCACACCGAAAATTGCATGAGAATGATAGAGGCTGGGGCTGTTACAGCGCTTGTAAGCGCATTGGGAAACGAAGGGGTTGCTGAGGAAGCAGCTGGTGCATTGGCATTGGTTGTCAGGCAACCTATTGGGGCTAAAGAGGTGGCAAAAGAGGACATTGCAGTGGCAGGTTTGATAGGGATGATGCGTTGCGGGACTCCAAGGGGTAAAGAAAATGCAGTTGCAGCTTTGCTCGAGCTGTGCCGATGCGGTGGAGCTGCTGCAACTGAGAGGGTGGTTAAGGCACCAGCTTTGGCGGGGTTACTTCAGATGTTGCTTTTTACCGGTACAAAGCGGGCAAGAAGAAAGGCAGCTTCTCTTGCTAGAGTGTTCCAAAGGTGTGAGAATGCTGCAGCAATGCATTTTGGTGGTCCCGGCGTGGGATATGCACTTGCAGGTAACTCGACTGCAAATAGGGATTCGAGTTTCGTAGGTGATGTTTCAGTGCCGATGCCCATTTCCGTGCATGTTTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009814 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0070647 GO:0071704 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

720

Amino Acids

78.86

Weight (kDa)

5.89

Isoelectric Point (pI)

46.22

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
MCAfunc	PF19584	54 - 176	8.9e-06	MCAfunc domain
PUB10_N	PF25368	56 - 166	4.5e-14	PUB10-like, N-terminal domain
U-box	PF04564	291 - 358	8.6e-19	U-box domain
ARM_ARMC5	PF24768	391 - 574	1e-10	ARMC5
ARM_PUB	PF25598	411 - 673	1.9e-20	Plant U-box protein ARM repeat region
Arm	PF00514	426 - 463	2e-09	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014286)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29340
fragaria_vesca	FvH4_3g07220
malus_domestica	MD00G1008100.v1.1 MD10G1274600.v1.1
prunus_persica	Prupe.4G067800_v2.0.a1
pyrus_communis	pycom05g27330 pycom10g22910
rosa_chinensis	RchiOBHm_Chr5g0011931
rosa_laevigata	RLG00000031870
rosa_multiflora	Rmu_ssc0000228.1_g000008
rosa_roxburghii	Rroxscaffold_1G00064660
rosa_rugosa	Rorug04G0460100
rosa_samantha	Rh5AG091600 Rh5BG087400 Rh5CG101300 Rh5DG088000
rosa_wichuraiana	Rw5G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	866
AatII	GACGTC	2 cut(s)	90, 438
Acc16I	TGCGCA	1 cut(s)	325
Acc36I	ACCTGC	3 cut(s)	741, 1802, 2069
Acc65I	GGTACC	1 cut(s)	1595
AccB1I	GGYRCC	2 cut(s)	1595, 1927
AccB7I	CCANNNNNTGG	1 cut(s)	1204
AccI	GTMKAC	1 cut(s)	1643
AccII	CGCG	2 cut(s)	1184, 1245
AclWI	GGATC	2 cut(s)	294, 1222
AcoI	YGGCCR	2 cut(s)	373, 588
AcsI	RAATTY	6 cut(s)	172, 399, 560, 1054, 1086, 1360
AcuI	CTGAAG	2 cut(s)	338, 1934
AcyI	GRCGYC	4 cut(s)	87, 435, 633, 1617
AdeI	CACNNNGTG	1 cut(s)	1223
AfaI	GTAC	6 cut(s)	99, 256, 296, 991, 1597, 1972
AfeI	AGCGCT	1 cut(s)	1695
AfiI	CCNNNNNNNGG	9 cut(s)	165, 189, 371, 597, 741, 1004, 1204, 1417, 2063
AgeI	ACCGGT	1 cut(s)	1967
AgsI	TTSAA	3 cut(s)	614, 773, 788
AjiI	CACGTC	1 cut(s)	454
AjnI	CCWGG	2 cut(s)	183, 488
AjuI	GAANNNNNNNTTGG	2 cut(s)	780, 812
AleI	CACNNNNGTG	1 cut(s)	690
Alw21I	GWGCWC	5 cut(s)	216, 474, 720, 1076, 1228
Alw26I	GTCTC	6 cut(s)	115, 513, 624, 851, 1341, 1517
Alw44I	GTGCAC	2 cut(s)	1072, 1224
AlwI	GGATC	2 cut(s)	294, 1222
Ama87I	CYCGRG	1 cut(s)	1877
Aor51HI	AGCGCT	1 cut(s)	1695
AoxI	GGCC	5 cut(s)	373, 486, 571, 588, 732
ApaLI	GTGCAC	2 cut(s)	1072, 1224
ApoI	RAATTY	6 cut(s)	172, 399, 560, 1054, 1086, 1360
AsiGI	ACCGGT	1 cut(s)	1967
Asp718I	GGTACC	1 cut(s)	1595
AspLEI	GCGC	3 cut(s)	326, 1696, 1706
AspS9I	GGNCC	4 cut(s)	180, 571, 1440, 2054
AsuC2I	CCSGG	3 cut(s)	575, 1303, 2058
AsuHPI	GGTGA	5 cut(s)	36, 704, 934, 1209, 2129
AvaI	CYCGRG	1 cut(s)	1877
AvaII	GGWCC	3 cut(s)	180, 1440, 2054
BaeGI	GKGCMC	2 cut(s)	1076, 1228
BanI	GGYRCC	2 cut(s)	1595, 1927
BanII	GRGCYC	2 cut(s)	474, 705
BauI	CACGAG	1 cut(s)	209
BbsI	GAAGAC	2 cut(s)	783, 829
Bbv12I	GWGCWC	5 cut(s)	216, 474, 720, 1076, 1228
BbvCI	CCTCAGC	2 cut(s)	530, 1728
BccI	CCATC	4 cut(s)	167, 369, 421, 1205
BceAI	ACGGC	4 cut(s)	235, 273, 1217, 1604
BcgI	CGANNNNNNTGC	4 cut(s)	1706, 1740, 2067, 2101
BciT130I	CCWGG	2 cut(s)	185, 490
BclI	TGATCA	2 cut(s)	924, 1059
BcnI	CCSGG	3 cut(s)	575, 1303, 2058
BcoDI	GTCTC	6 cut(s)	115, 513, 624, 851, 1341, 1517
BfaI	CTAG	1 cut(s)	2007
BfmI	CTRYAG	6 cut(s)	476, 482, 1233, 1494, 1518, 2034
BfoI	RGCGCY	1 cut(s)	1697
BfuAI	ACCTGC	3 cut(s)	741, 1802, 2069
BglI	GCCNNNNNGGC	1 cut(s)	494
BglII	AGATCT	1 cut(s)	201
Bme1390I	CCNGG	5 cut(s)	185, 490, 575, 1303, 2058
Bme18I	GGWCC	3 cut(s)	180, 1440, 2054
BmeT110I	CYCGRG	1 cut(s)	1877
BmgBI	CACGTC	1 cut(s)	454
BmgT120I	GGNCC	4 cut(s)	180, 571, 1440, 2054
BmiI	GGNNCC	7 cut(s)	182, 579, 918, 1441, 1597, 1929, 2056
BmrFI	CCNGG	5 cut(s)	185, 490, 575, 1303, 2058
BmsI	GCATC	6 cut(s)	18, 1105, 1127, 1818, 1880, 2124
BoxI	GACNNNNGTC	1 cut(s)	107
BpiI	GAAGAC	2 cut(s)	783, 829
BpmI	CTGGAG	3 cut(s)	74, 1086, 1226
Bpu10I	CCTNAGC	2 cut(s)	530, 1728
BpuMI	CCSGG	3 cut(s)	575, 1303, 2058
Bsa29I	ATCGAT	2 cut(s)	26, 1388
BsaBI	GATNNNNATC	1 cut(s)	390
BsaHI	GRCGYC	4 cut(s)	87, 435, 633, 1617
BsaJI	CCNNGG	4 cut(s)	183, 1182, 1302, 1844
BsaWI	WCCGGW	2 cut(s)	70, 1967
Bsc4I	CCNNNNNNNGG	9 cut(s)	165, 189, 371, 597, 741, 1004, 1204, 1417, 2063
Bse118I	RCCGGY	2 cut(s)	919, 1967
Bse1I	ACTGG	1 cut(s)	333
Bse3DI	GCAATG	4 cut(s)	1290, 1380, 1800, 2046
Bse8I	GATNNNNATC	1 cut(s)	390
BseBI	CCWGG	2 cut(s)	185, 490
BseCI	ATCGAT	2 cut(s)	26, 1388
BseDI	CCNNGG	4 cut(s)	183, 1182, 1302, 1844
BseGI	GGATG	4 cut(s)	159, 361, 1216, 1830
BseJI	GATNNNNATC	1 cut(s)	390
BseLI	CCNNNNNNNGG	9 cut(s)	165, 189, 371, 597, 741, 1004, 1204, 1417, 2063
BseMI	GCAATG	4 cut(s)	1290, 1380, 1800, 2046
BseMII	CTCAG	8 cut(s)	156, 218, 521, 857, 1448, 1467, 1719, 1902
BseNI	ACTGG	1 cut(s)	333
BseRI	GAGGAG	3 cut(s)	302, 539, 683
BseSI	GKGCMC	2 cut(s)	1076, 1228
BseX3I	CGGCCG	1 cut(s)	588
BseYI	CCCAGC	2 cut(s)	1019, 1679
BsgI	GTGCAG	1 cut(s)	1143
Bsh1236I	CGCG	2 cut(s)	1184, 1245
Bsh1285I	CGRYCG	2 cut(s)	591, 1182
BshFI	GGCC	5 cut(s)	375, 488, 573, 590, 734
BshNI	GGYRCC	2 cut(s)	1595, 1927
BshTI	ACCGGT	1 cut(s)	1967
BshVI	ATCGAT	2 cut(s)	26, 1388
BsiEI	CGRYCG	2 cut(s)	591, 1182
BsiHKAI	GWGCWC	5 cut(s)	216, 474, 720, 1076, 1228
BsiHKCI	CYCGRG	1 cut(s)	1877
BsiSI	CCGG	7 cut(s)	71, 372, 574, 920, 1302, 1968, 2058
BslFI	GGGAC	4 cut(s)	166, 245, 1852, 2040
BslI	CCNNNNNNNGG	9 cut(s)	165, 189, 371, 597, 741, 1004, 1204, 1417, 2063
BsmAI	GTCTC	6 cut(s)	115, 513, 624, 851, 1341, 1517
BsmBI	CGTCTC	3 cut(s)	513, 624, 1341
BsmFI	GGGAC	4 cut(s)	166, 245, 1852, 2040
BsmI	GAATGC	2 cut(s)	1495, 2035
BsnI	GGCC	5 cut(s)	375, 488, 573, 590, 734
BsoBI	CYCGRG	1 cut(s)	1877
Bsp1286I	GDGCHC	6 cut(s)	216, 474, 705, 720, 1076, 1228
Bsp1407I	TGTACA	2 cut(s)	97, 294
Bsp143I	GATC	6 cut(s)	201, 299, 924, 949, 1059, 1214
Bsp68I	TCGCGA	1 cut(s)	1245
BspANI	GGCC	5 cut(s)	375, 488, 573, 590, 734
BspCNI	CTCAG	8 cut(s)	155, 219, 522, 856, 1449, 1468, 1720, 1903
BspDI	ATCGAT	2 cut(s)	26, 1388
BspFNI	CGCG	2 cut(s)	1184, 1245
BspHI	TCATGA	1 cut(s)	1525
BspLI	GGNNCC	7 cut(s)	182, 579, 918, 1441, 1597, 1929, 2056
BspMAI	CTGCAG	4 cut(s)	480, 1498, 1522, 2038
BspMI	ACCTGC	3 cut(s)	741, 1802, 2069
BspPI	GGATC	2 cut(s)	294, 1222
BspT107I	GGYRCC	2 cut(s)	1595, 1927
BsrDI	GCAATG	4 cut(s)	1290, 1380, 1800, 2046
BsrFI	RCCGGY	2 cut(s)	919, 1967
BsrGI	TGTACA	2 cut(s)	97, 294
BsrI	ACTGG	1 cut(s)	333
BssAI	RCCGGY	2 cut(s)	919, 1967
BssECI	CCNNGG	4 cut(s)	183, 1182, 1302, 1844
BssMI	GATC	6 cut(s)	201, 299, 924, 949, 1059, 1214
BssNI	GRCGYC	4 cut(s)	87, 435, 633, 1617
BssSI	CACGAG	1 cut(s)	209
BssT1I	CCWWGG	1 cut(s)	1844
Bst2BI	CACGAG	1 cut(s)	209
Bst2UI	CCWGG	2 cut(s)	185, 490
Bst4CI	ACNGT	2 cut(s)	1283, 1584
Bst6I	CTCTTC	3 cut(s)	823, 1267, 1532
BstACI	GRCGYC	4 cut(s)	87, 435, 633, 1617
BstAPI	GCANNNNNTGC	1 cut(s)	1742
BstAUI	TGTACA	2 cut(s)	97, 294
BstC8I	GCNNGC	3 cut(s)	1243, 1260, 1980
BstDEI	CTNAG	9 cut(s)	142, 227, 530, 843, 1152, 1457, 1476, 1728, 1911
BstDSI	CCRYGG	1 cut(s)	1182
BstEII	GGTNACC	1 cut(s)	42
BstF5I	GGATG	4 cut(s)	159, 361, 1216, 1830
BstFNI	CGCG	2 cut(s)	1184, 1245
BstH2I	RGCGCY	1 cut(s)	1697
BstHHI	GCGC	3 cut(s)	326, 1696, 1706
BstKTI	GATC	6 cut(s)	204, 302, 927, 952, 1062, 1217
BstMAI	GTCTC	6 cut(s)	115, 513, 624, 851, 1341, 1517
BstMBI	GATC	6 cut(s)	201, 299, 924, 949, 1059, 1214
BstMCI	CGRYCG	2 cut(s)	591, 1182
BstNI	CCWGG	2 cut(s)	185, 490
BstNSI	RCATGY	1 cut(s)	2153
BstPAI	GACNNNNGTC	1 cut(s)	107
BstPI	GGTNACC	1 cut(s)	42
BstSCI	CCNGG	5 cut(s)	183, 488, 573, 1301, 2056
BstSFI	CTRYAG	6 cut(s)	476, 482, 1233, 1494, 1518, 2034
BstSLI	GKGCMC	2 cut(s)	1076, 1228
BstUI	CGCG	2 cut(s)	1184, 1245
BstV2I	GAAGAC	2 cut(s)	783, 829
BstX2I	RGATCY	2 cut(s)	201, 299
BstXI	CCANNNNNNTGG	1 cut(s)	901
BstYI	RGATCY	2 cut(s)	201, 299
BstZI	CGGCCG	1 cut(s)	588
Bsu15I	ATCGAT	2 cut(s)	26, 1388
BsuRI	GGCC	5 cut(s)	375, 488, 573, 590, 734
BsuTUI	ATCGAT	2 cut(s)	26, 1388
BtgI	CCRYGG	1 cut(s)	1182
BtrI	CACGTC	1 cut(s)	454
BtsCI	GGATG	4 cut(s)	159, 361, 1216, 1830
BtsI	GCAGTG	3 cut(s)	1073, 1074, 1812
BtsIMutI	CAGTG	4 cut(s)	1073, 1074, 1812, 2133
BtuMI	TCGCGA	1 cut(s)	1245
BveI	ACCTGC	3 cut(s)	741, 1802, 2069
Cac8I	GCNNGC	3 cut(s)	1243, 1260, 1980
CciI	TCATGA	1 cut(s)	1525
CfoI	GCGC	3 cut(s)	326, 1696, 1706
Cfr10I	RCCGGY	2 cut(s)	919, 1967
Cfr13I	GGNCC	4 cut(s)	180, 571, 1440, 2054
Cfr42I	CCGCGG	1 cut(s)	1185
ClaI	ATCGAT	2 cut(s)	26, 1388
CseI	GACGC	3 cut(s)	248, 641, 1625
Csp6I	GTAC	6 cut(s)	98, 255, 295, 990, 1596, 1971
CspAI	ACCGGT	1 cut(s)	1967
CviAII	CATG	5 cut(s)	1063, 1221, 1526, 1663, 2150
CviQI	GTAC	6 cut(s)	98, 255, 295, 990, 1596, 1971
DdeI	CTNAG	9 cut(s)	142, 227, 530, 843, 1152, 1457, 1476, 1728, 1911
DpnI	GATC	6 cut(s)	203, 301, 926, 951, 1061, 1216
DpnII	GATC	6 cut(s)	201, 299, 924, 949, 1059, 1214
DraIII	CACNNNGTG	1 cut(s)	1223
EaeI	YGGCCR	2 cut(s)	373, 588
EagI	CGGCCG	1 cut(s)	588
Eam1104I	CTCTTC	3 cut(s)	823, 1267, 1532
EarI	CTCTTC	3 cut(s)	823, 1267, 1532
EciI	GGCGGA	2 cut(s)	52, 184
Ecl136II	GAGCTC	1 cut(s)	472
EclXI	CGGCCG	1 cut(s)	588
Eco130I	CCWWGG	1 cut(s)	1844
Eco24I	GRGCYC	2 cut(s)	474, 705
Eco47I	GGWCC	3 cut(s)	180, 1440, 2054
Eco47III	AGCGCT	1 cut(s)	1695
Eco52I	CGGCCG	1 cut(s)	588
Eco53kI	GAGCTC	1 cut(s)	472
Eco57I	CTGAAG	2 cut(s)	338, 1934
Eco88I	CYCGRG	1 cut(s)	1877
Eco91I	GGTNACC	1 cut(s)	42
EcoICRI	GAGCTC	1 cut(s)	472
EcoO109I	RGGNCCY	1 cut(s)	180
EcoO65I	GGTNACC	1 cut(s)	42
EcoRI	GAATTC	4 cut(s)	172, 560, 1086, 1360
EcoRII	CCWGG	2 cut(s)	183, 488
EcoT14I	CCWWGG	1 cut(s)	1844
EcoT22I	ATGCAT	2 cut(s)	1120, 2046
EcoT38I	GRGCYC	2 cut(s)	474, 705
ErhI	CCWWGG	1 cut(s)	1844
Esp3I	CGTCTC	3 cut(s)	513, 624, 1341
FaeI	CATG	5 cut(s)	1066, 1224, 1529, 1666, 2153
FaqI	GGGAC	4 cut(s)	166, 245, 1852, 2040
FatI	CATG	5 cut(s)	1062, 1220, 1525, 1662, 2149
FauI	CCCGC	4 cut(s)	1177, 1829, 1933, 1971
FbaI	TGATCA	2 cut(s)	924, 1059
FblI	GTMKAC	1 cut(s)	1643
FokI	GGATG	4 cut(s)	146, 348, 1223, 1837
FriOI	GRGCYC	2 cut(s)	474, 705
FspBI	CTAG	1 cut(s)	2007
FspI	TGCGCA	1 cut(s)	325
GlaI	GCGC	3 cut(s)	325, 1695, 1705
GsaI	CCCAGC	2 cut(s)	1023, 1683
GsuI	CTGGAG	3 cut(s)	74, 1086, 1226
HaeII	RGCGCY	1 cut(s)	1697
HaeIII	GGCC	5 cut(s)	375, 488, 573, 590, 734
HapII	CCGG	7 cut(s)	71, 372, 574, 920, 1302, 1968, 2058
HgaI	GACGC	3 cut(s)	248, 641, 1625
HhaI	GCGC	3 cut(s)	326, 1696, 1706
Hin1I	GRCGYC	4 cut(s)	87, 435, 633, 1617
Hin1II	CATG	5 cut(s)	1066, 1224, 1529, 1666, 2153
Hin6I	GCGC	3 cut(s)	324, 1694, 1704
HinP1I	GCGC	3 cut(s)	324, 1694, 1704
HincII	GTYRAC	2 cut(s)	76, 1644
HindII	GTYRAC	2 cut(s)	76, 1644
HindIII	AAGCTT	1 cut(s)	1569
HpaII	CCGG	7 cut(s)	71, 372, 574, 920, 1302, 1968, 2058
HphI	GGTGA	5 cut(s)	36, 704, 934, 1209, 2129
Hpy166II	GTNNAC	7 cut(s)	76, 255, 682, 1074, 1226, 1420, 1644
Hpy8I	GTNNAC	7 cut(s)	76, 255, 682, 1074, 1226, 1420, 1644
Hpy99I	CGWCG	6 cut(s)	89, 92, 119, 242, 440, 515
HpyCH4III	ACNGT	2 cut(s)	1283, 1584
HpyCH4IV	ACGT	6 cut(s)	87, 417, 435, 453, 696, 944
HpyF3I	CTNAG	9 cut(s)	142, 227, 530, 843, 1152, 1457, 1476, 1728, 1911
HpySE526I	ACGT	6 cut(s)	87, 417, 435, 453, 696, 944
Hsp92I	GRCGYC	4 cut(s)	87, 435, 633, 1617
Hsp92II	CATG	5 cut(s)	1066, 1224, 1529, 1666, 2153
HspAI	GCGC	3 cut(s)	324, 1694, 1704
KpnI	GGTACC	1 cut(s)	1599
Ksp22I	TGATCA	2 cut(s)	924, 1059
KspI	CCGCGG	1 cut(s)	1185
Kzo9I	GATC	6 cut(s)	201, 299, 924, 949, 1059, 1214
LmnI	GCTCC	7 cut(s)	73, 460, 477, 526, 670, 916, 1898
LweI	GCATC	6 cut(s)	18, 1105, 1127, 1818, 1880, 2124
MaeI	CTAG	1 cut(s)	2007
MaeII	ACGT	6 cut(s)	87, 417, 435, 453, 696, 944
MalI	GATC	6 cut(s)	203, 301, 926, 951, 1061, 1216
MboI	GATC	6 cut(s)	201, 299, 924, 949, 1059, 1214
MboII	GAAGA	9 cut(s)	785, 788, 834, 840, 989, 1284, 1436, 1549, 1998
MflI	RGATCY	2 cut(s)	201, 299
MhlI	GDGCHC	6 cut(s)	216, 474, 705, 720, 1076, 1228
MlyI	GAGTC	4 cut(s)	227, 578, 1639, 1834
MmeI	TCCRAC	4 cut(s)	108, 261, 418, 1222
Mph1103I	ATGCAT	2 cut(s)	1120, 2046
MseI	TTAA	3 cut(s)	1449, 1635, 1923
MslI	CAYNNNNRTG	5 cut(s)	690, 1667, 1805, 2049, 2144
MspA1I	CMGCKG	3 cut(s)	166, 1184, 1739
MspI	CCGG	7 cut(s)	71, 372, 574, 920, 1302, 1968, 2058
MspR9I	CCNGG	5 cut(s)	185, 490, 575, 1303, 2058
Mva1269I	GAATGC	2 cut(s)	1495, 2035
MvaI	CCWGG	2 cut(s)	185, 490
MvnI	CGCG	2 cut(s)	1184, 1245
NciI	CCSGG	3 cut(s)	575, 1303, 2058
NdeII	GATC	6 cut(s)	201, 299, 924, 949, 1059, 1214
NlaIII	CATG	5 cut(s)	1066, 1224, 1529, 1666, 2153
NlaIV	GGNNCC	7 cut(s)	182, 579, 918, 1441, 1597, 1929, 2056
NmeAIII	GCCGAG	2 cut(s)	566, 880
NmuCI	GTSAC	4 cut(s)	42, 692, 1466, 1621
NruI	TCGCGA	1 cut(s)	1245
NsbI	TGCGCA	1 cut(s)	325
NsiI	ATGCAT	2 cut(s)	1120, 2046
NspI	RCATGY	1 cut(s)	2153
OliI	CACNNNNGTG	1 cut(s)	690
PaeR7I	CTCGAG	1 cut(s)	1877
PagI	TCATGA	1 cut(s)	1525
PcsI	WCGNNNNNNNCGW	3 cut(s)	519, 693, 1478
PctI	GAATGC	2 cut(s)	1495, 2035
PfeI	GAWTC	7 cut(s)	391, 625, 788, 871, 1039, 1563, 2102
PflMI	CCANNNNNTGG	1 cut(s)	1204
PinAI	ACCGGT	1 cut(s)	1967
PleI	GAGTC	4 cut(s)	227, 578, 1639, 1834
PpsI	GAGTC	4 cut(s)	227, 578, 1639, 1834
PpuMI	RGGWCCY	1 cut(s)	180
PshAI	GACNNNNGTC	1 cut(s)	107
PsiI	TTATAA	1 cut(s)	866
Psp124BI	GAGCTC	1 cut(s)	474
Psp5II	RGGWCCY	1 cut(s)	180
Psp6I	CCWGG	2 cut(s)	183, 488
PspEI	GGTNACC	1 cut(s)	42
PspFI	CCCAGC	2 cut(s)	1019, 1679
PspGI	CCWGG	2 cut(s)	183, 488
PspN4I	GGNNCC	7 cut(s)	182, 579, 918, 1441, 1597, 1929, 2056
PspPI	GGNCC	4 cut(s)	180, 571, 1440, 2054
PspPPI	RGGWCCY	1 cut(s)	180
PspXI	VCTCGAGB	1 cut(s)	1877
PstI	CTGCAG	4 cut(s)	480, 1498, 1522, 2038
PsuI	RGATCY	2 cut(s)	201, 299
PvuII	CAGCTG	1 cut(s)	1739
RruI	TCGCGA	1 cut(s)	1245
RsaI	GTAC	6 cut(s)	99, 256, 296, 991, 1597, 1972
RsaNI	GTAC	6 cut(s)	98, 255, 295, 990, 1596, 1971
RseI	CAYNNNNRTG	5 cut(s)	690, 1667, 1805, 2049, 2144
SacI	GAGCTC	1 cut(s)	474
SacII	CCGCGG	1 cut(s)	1185
SalI	GTCGAC	1 cut(s)	1642
SaqAI	TTAA	3 cut(s)	1449, 1635, 1923
Sau3AI	GATC	6 cut(s)	201, 299, 924, 949, 1059, 1214
Sau96I	GGNCC	4 cut(s)	180, 571, 1440, 2054
SchI	GAGTC	4 cut(s)	227, 578, 1639, 1834
ScrFI	CCNGG	5 cut(s)	185, 490, 575, 1303, 2058
SduI	GDGCHC	6 cut(s)	216, 474, 705, 720, 1076, 1228
SfaNI	GCATC	6 cut(s)	18, 1105, 1127, 1818, 1880, 2124
SfcI	CTRYAG	6 cut(s)	476, 482, 1233, 1494, 1518, 2034
Sfr274I	CTCGAG	1 cut(s)	1877
Sfr303I	CCGCGG	1 cut(s)	1185
SgrBI	CCGCGG	1 cut(s)	1185
SinI	GGWCC	3 cut(s)	180, 1440, 2054
SlaI	CTCGAG	1 cut(s)	1877
SmiMI	CAYNNNNRTG	5 cut(s)	690, 1667, 1805, 2049, 2144
SmlI	CTYRAG	1 cut(s)	1877
SmoI	CTYRAG	1 cut(s)	1877
SspMI	CTAG	1 cut(s)	2007
SstI	GAGCTC	1 cut(s)	474
StyD4I	CCNGG	5 cut(s)	183, 488, 573, 1301, 2056
StyI	CCWWGG	1 cut(s)	1844
TaaI	ACNGT	2 cut(s)	1283, 1584
TaiI	ACGT	6 cut(s)	90, 420, 438, 456, 699, 947
TatI	WGTACW	3 cut(s)	97, 294, 989
TauI	GCSGC	3 cut(s)	14, 169, 1139
TfiI	GAWTC	7 cut(s)	391, 625, 788, 871, 1039, 1563, 2102
Tru1I	TTAA	3 cut(s)	1449, 1635, 1923
Tru9I	TTAA	3 cut(s)	1449, 1635, 1923
TscAI	CASTG	4 cut(s)	1073, 1081, 1812, 2133
TseFI	GTSAC	4 cut(s)	42, 692, 1466, 1621
Tsp45I	GTSAC	4 cut(s)	42, 692, 1466, 1621
TspDTI	ATGAA	5 cut(s)	404, 496, 1279, 1410, 1514
TspGWI	ACGGA	3 cut(s)	103, 1080, 2134
TspRI	CASTG	4 cut(s)	1073, 1081, 1812, 2133
Van91I	CCANNNNNTGG	1 cut(s)	1204
VneI	GTGCAC	2 cut(s)	1072, 1224
VpaK11BI	GGWCC	3 cut(s)	180, 1440, 2054
XapI	RAATTY	6 cut(s)	172, 399, 560, 1054, 1086, 1360
XceI	RCATGY	1 cut(s)	2153
XhoI	CTCGAG	1 cut(s)	1877
XmiI	GTMKAC	1 cut(s)	1643
XspI	CTAG	1 cut(s)	2007
ZraI	GACGTC	2 cut(s)	88, 436
Zsp2I	ATGCAT	2 cut(s)	1120, 2046

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.