Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr10

Orthogroup ID

OG0014286

Physical Location & Seq

Genomic Coordinates Reverse (-)

36604548 .. 36607319

Gene Structure 2772 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD10G1274600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2133 bp

ATGGCTTCGGCGGCGATATTCTCATCGATGCGCCGGCGGAGGTCACCTTCTCTGGAGGCGTTTTTAGCTCCGGTTGACCTCTCTGACGTGGCCCTTGTACAGACCCTTGTCTCGGTCACCGCCGAGATAGTTTCCTCCTTCTCAGACAAGTCTTTCTCCTTCCAGCGGCGGAATTCGAGGTCGCTGGTGCGGAAAGTCGAAATCTTTCTCGTGCTCTTGGAGTATCTGAGGGACTTCGGGGCTGCTATGCCGTGGACGGCGGTCATCTGCCTGAAAGAGCTCTACTTGCTGCTATACAGATCCAAAATACTCCTCGATTACTGCGCCCGGTCAAGCAAGTTGTGGCTTTTGCTTCAGAACCATCCAATTTCCGGCCATTTTCACGATTTGAATCAGGAAATTTCGACCCTTTTGGACGTGTTTCCGATGGATGACGTCGAATTGGGGGAGGACGTGAGGGAACAGATTGAGCTCCTGCAGCGACAGGCCAGGAGGGCGATGCTGTTCATCGAAGAAGGAGATGAAGAATTAAGGGTTCAGTTCTTTTCGTTCCTTGACGAATTCGAGAAGGGCCGGGTTCCTGACTCGGCCGATTTGTGGTTGTTTTTCGTTGAAAGATTGGGGATTCGAGACGCCAAGAGTTGCCGGCACGAAATCGAGTTCTTGGAGGAGCAGATTGTGAACCACGAAGGTGACGTTGAGCCCACCGTTTCGGTGCTTAATGGGTTTGTGGCATTCACTCGGTATTGCAGGTTTTTGCTTTTCGGTTTCGAAGAAGACGAGGTTGAATTGGGGATTGGGAACAATCAGAGGAAGCCCCGAAAAGGGTTGATTGCTCAGGAGATTGCGGAGACTTTTATAACGATTCCGAAGGACTTTTGCTGCCCCATATCATTGGATTTGATGAGAGAGCCAGTGATCATTGCGACAGGACAGACATATGATCGGAGTTCAATTTCGAGGTGGCTGGAAGAAGGGAATTGTACTTGTCCGAAAACAGGGCAAATGCTGCCCAGCACTCGCCTGGTTCCGAATCGGGCTTTGAGGAATTTGATCATGCAGTGGTGCACCGCTTATGGAATTCCTTACGACCCTCCAGAGTCTGCATATGCATCAGCAGACAGTTTTGCAGCTGCTTCTCCCTCTAAGGCTGCTGTTGCAGCCAACAAAGCGACCGCGGGGCTTCTCATCCAACGGCTGGAGGATGGATCACCATGCGCACAAACTATAGCTGCTCGTGAAATTCGATTGCTGGCAAAAACAGGAAGAGAAAACCGCGCTTTCATTGCGGAAGCTGGGGCAATTCCCCATCTTTGTAAGTTACTCTCGTCTCCAAACTCTGTGGCCCAAGAGAATTCTGTGACTGCAATGCTCAACTTATCAATATATGATAAAAACAAAAGCCGAATTATGGACGAAGAAGGGTGTTTGGGGTCCATTGTTAATGTTCTGAGGTTTGGGCACACGACTGAGGCGAGGGAGAATGCTGCAGCAACTTTGTTTAGTCTATCTGCAGTTCATGACTATAAGAAGAGAATAGCGGACGAGGAAGGGGGAATCGAAGCTTTGGCAGGATTGTTGAGAGAGGGTACCCCAAGAGGAAAGAAGGATGCCGTGACAGCTTTGTATAACCTGTCTACTCACACCGAAAATTGCACGAGAATGATAGAGGCTGGGGCTGTTACAGCGCTTGTCAGTGCATTAGGAAACGAAGTGGTTGCTGAGGAAGCAGCTGGTGCATTGGCATTGGTTGTCAGGCAGCCTATTGGGGCAAAAGCGGTGGCGAATGAGGACCTGGCAGTGGCAGGTTTGATAGGGATGATGCGTGGCGGGACTCCAAGGGGTAAAGAAAATGCAGTTGCAGCTTTGCTCGAGCTGTGCCGAAGCGGTGGAACTGCTGCAACTGAGATGGTAGTTAAGGCACCAGCTTTGGCAGGGTTACTTCAGACGTTGCTTTTTACTGGTACAAAGCGGGCAAGAAGAAAGGCAGCTTCTCTTGCTAGGGTGTTCCAAAGGTGTGAGAATGCTGCAGCAATGCATTTTGGTGGTCTCGGCGTGGGATATGCACTTGCAGGTAACTCAGCTGCGAATAGGGATTCGAGTTTCGTAGGGACGTACGTTGTAATGCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009814 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0070647 GO:0071704 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

711

Amino Acids

77.75

Weight (kDa)

5.87

Isoelectric Point (pI)

46.46

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB10_N	PF25368	56 - 166	2.4e-14	PUB10-like, N-terminal domain
U-box	PF04564	289 - 356	1.2e-19	U-box domain
ARM_ARMC5	PF24768	381 - 569	2.5e-09	ARMC5
ARM_PUB	PF25598	409 - 671	2.4e-19	Plant U-box protein ARM repeat region
Arm	PF00514	424 - 461	1.5e-08	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014286)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29340
fragaria_vesca	FvH4_3g07220
malus_domestica	MD00G1008100.v1.1 MD10G1274600.v1.1
prunus_persica	Prupe.4G067800_v2.0.a1
pyrus_communis	pycom05g27330 pycom10g22910
rosa_chinensis	RchiOBHm_Chr5g0011931
rosa_laevigata	RLG00000031870
rosa_multiflora	Rmu_ssc0000228.1_g000008
rosa_roxburghii	Rroxscaffold_1G00064660
rosa_rugosa	Rorug04G0460100
rosa_samantha	Rh5AG091600 Rh5BG087400 Rh5CG101300 Rh5DG088000
rosa_wichuraiana	Rw5G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	860
AatII	GACGTC	1 cut(s)	438
Acc16I	TGCGCA	1 cut(s)	1219
Acc36I	ACCTGC	3 cut(s)	741, 1796, 2063
Acc65I	GGTACC	1 cut(s)	1589
AccB1I	GGYRCC	2 cut(s)	1589, 1921
AccB7I	CCANNNNNTGG	1 cut(s)	1198
AccI	GTMKAC	1 cut(s)	1637
AccII	CGCG	2 cut(s)	1178, 1278
AclWI	GGATC	2 cut(s)	294, 1216
AcoI	YGGCCR	2 cut(s)	373, 588
AcsI	RAATTY	7 cut(s)	172, 399, 560, 1048, 1080, 1242, 1354
AcuI	CTGAAG	2 cut(s)	338, 1928
AcyI	GRCGYC	2 cut(s)	435, 633
AfaI	GTAC	5 cut(s)	99, 985, 1591, 1966, 2117
AfeI	AGCGCT	1 cut(s)	1689
AflIII	ACRYGT	1 cut(s)	417
AgsI	TTSAA	4 cut(s)	391, 614, 788, 954
AjiI	CACGTC	3 cut(s)	88, 418, 454
AjnI	CCWGG	3 cut(s)	488, 1023, 1794
AjuI	GAANNNNNNNTTGG	2 cut(s)	780, 812
AleI	CACNNNNGTG	1 cut(s)	690
Alw21I	GWGCWC	4 cut(s)	216, 282, 474, 1070
Alw26I	GTCTC	5 cut(s)	115, 624, 845, 1335, 2054
Alw44I	GTGCAC	1 cut(s)	1066
AlwI	GGATC	2 cut(s)	294, 1216
AlwNI	CAGNNNCTG	1 cut(s)	1103
Ama87I	CYCGRG	1 cut(s)	1871
Aor51HI	AGCGCT	1 cut(s)	1689
AoxI	GGCC	6 cut(s)	90, 373, 486, 571, 588, 1344
ApaLI	GTGCAC	1 cut(s)	1066
ApoI	RAATTY	7 cut(s)	172, 399, 560, 1048, 1080, 1242, 1354
Asp700I	GAANNNNTTC	1 cut(s)	204
Asp718I	GGTACC	1 cut(s)	1589
AspLEI	GCGC	5 cut(s)	33, 326, 1220, 1280, 1690
AspS9I	GGNCC	5 cut(s)	91, 571, 1345, 1434, 1792
AsuC2I	CCSGG	2 cut(s)	328, 575
AsuHPI	GGTGA	4 cut(s)	36, 109, 704, 1203
AsuII	TTCGAA	1 cut(s)	771
AvaI	CYCGRG	1 cut(s)	1871
AvaII	GGWCC	2 cut(s)	1434, 1792
BaeGI	GKGCMC	2 cut(s)	1070, 1464
BanI	GGYRCC	2 cut(s)	1589, 1921
BanII	GRGCYC	3 cut(s)	282, 474, 705
BauI	CACGAG	3 cut(s)	209, 1236, 1657
BbsI	GAAGAC	1 cut(s)	783
Bbv12I	GWGCWC	4 cut(s)	216, 282, 474, 1070
BbvCI	CCTCAGC	1 cut(s)	1722
BccI	CCATC	5 cut(s)	369, 421, 1199, 1317, 1903
BceAI	ACGGC	4 cut(s)	235, 273, 1211, 1598
BcgI	CGANNNNNNTGC	2 cut(s)	1700, 1734
BciT130I	CCWGG	3 cut(s)	490, 1025, 1796
BclI	TGATCA	2 cut(s)	918, 1053
BcnI	CCSGG	2 cut(s)	328, 575
BcoDI	GTCTC	5 cut(s)	115, 624, 845, 1335, 2054
BfaI	CTAG	1 cut(s)	2001
BfmI	CTRYAG	5 cut(s)	476, 1227, 1488, 1512, 2028
BfoI	RGCGCY	1 cut(s)	1691
BfuAI	ACCTGC	3 cut(s)	741, 1796, 2063
BglI	GCCNNNNNGGC	1 cut(s)	494
Bme1390I	CCNGG	5 cut(s)	328, 490, 575, 1025, 1796
Bme18I	GGWCC	2 cut(s)	1434, 1792
BmeT110I	CYCGRG	1 cut(s)	1871
BmgBI	CACGTC	3 cut(s)	88, 418, 454
BmgT120I	GGNCC	5 cut(s)	91, 571, 1345, 1434, 1792
BmiI	GGNNCC	5 cut(s)	579, 1029, 1435, 1591, 1923
BmrFI	CCNGG	5 cut(s)	328, 490, 575, 1025, 1796
BmsI	GCATC	5 cut(s)	18, 489, 1121, 1600, 1812
BoxI	GACNNNNGTC	2 cut(s)	107, 260
BpiI	GAAGAC	1 cut(s)	783
BpmI	CTGGAG	3 cut(s)	74, 1080, 1220
Bpu10I	CCTNAGC	2 cut(s)	837, 1722
Bpu14I	TTCGAA	1 cut(s)	771
BpuMI	CCSGG	2 cut(s)	328, 575
Bsa29I	ATCGAT	1 cut(s)	26
BsaBI	GATNNNNATC	1 cut(s)	390
BsaHI	GRCGYC	2 cut(s)	435, 633
BsaI	GGTCTC	1 cut(s)	2054
BsaJI	CCNNGG	3 cut(s)	251, 1176, 1838
BsaWI	WCCGGW	1 cut(s)	70
Bse118I	RCCGGY	2 cut(s)	33, 645
Bse1I	ACTGG	2 cut(s)	914, 1966
Bse3DI	GCAATG	4 cut(s)	921, 1284, 1374, 2040
Bse8I	GATNNNNATC	1 cut(s)	390
BseBI	CCWGG	3 cut(s)	490, 1025, 1796
BseCI	ATCGAT	1 cut(s)	26
BseDI	CCNNGG	3 cut(s)	251, 1176, 1838
BseGI	GGATG	6 cut(s)	361, 436, 1188, 1210, 1615, 1824
BseJI	GATNNNNATC	1 cut(s)	390
BseMI	GCAATG	4 cut(s)	921, 1284, 1374, 2040
BseMII	CTCAG	8 cut(s)	156, 218, 851, 1442, 1461, 1713, 1896, 2094
BseNI	ACTGG	2 cut(s)	914, 1966
BseRI	GAGGAG	2 cut(s)	302, 683
BseSI	GKGCMC	2 cut(s)	1070, 1464
BseX3I	CGGCCG	1 cut(s)	588
BseYI	CCCAGC	3 cut(s)	1013, 1295, 1673
Bsh1236I	CGCG	2 cut(s)	1178, 1278
Bsh1285I	CGRYCG	2 cut(s)	591, 1176
BshFI	GGCC	6 cut(s)	92, 375, 488, 573, 590, 1346
BshNI	GGYRCC	2 cut(s)	1589, 1921
BshVI	ATCGAT	1 cut(s)	26
BsiEI	CGRYCG	2 cut(s)	591, 1176
BsiHKAI	GWGCWC	4 cut(s)	216, 282, 474, 1070
BsiHKCI	CYCGRG	1 cut(s)	1871
BsiSI	CCGG	6 cut(s)	34, 71, 328, 372, 574, 646
BsiWI	CGTACG	1 cut(s)	2115
BslFI	GGGAC	3 cut(s)	245, 1846, 2125
BsmAI	GTCTC	5 cut(s)	115, 624, 845, 1335, 2054
BsmBI	CGTCTC	2 cut(s)	624, 1335
BsmFI	GGGAC	3 cut(s)	245, 1846, 2125
BsmI	GAATGC	3 cut(s)	734, 1489, 2029
BsnI	GGCC	6 cut(s)	92, 375, 488, 573, 590, 1346
Bso31I	GGTCTC	1 cut(s)	2054
BsoBI	CYCGRG	1 cut(s)	1871
Bsp119I	TTCGAA	1 cut(s)	771
Bsp1286I	GDGCHC	6 cut(s)	216, 282, 474, 705, 1070, 1464
Bsp1407I	TGTACA	1 cut(s)	97
Bsp143I	GATC	5 cut(s)	299, 918, 943, 1053, 1208
BspANI	GGCC	6 cut(s)	92, 375, 488, 573, 590, 1346
BspCNI	CTCAG	8 cut(s)	155, 219, 850, 1443, 1462, 1714, 1897, 2093
BspDI	ATCGAT	1 cut(s)	26
BspFNI	CGCG	2 cut(s)	1178, 1278
BspHI	TCATGA	1 cut(s)	1519
BspLI	GGNNCC	5 cut(s)	579, 1029, 1435, 1591, 1923
BspMAI	CTGCAG	4 cut(s)	480, 1492, 1516, 2032
BspMI	ACCTGC	3 cut(s)	741, 1796, 2063
BspPI	GGATC	2 cut(s)	294, 1216
BspT104I	TTCGAA	1 cut(s)	771
BspT107I	GGYRCC	2 cut(s)	1589, 1921
BspTNI	GGTCTC	1 cut(s)	2054
BsrDI	GCAATG	4 cut(s)	921, 1284, 1374, 2040
BsrFI	RCCGGY	2 cut(s)	33, 645
BsrGI	TGTACA	1 cut(s)	97
BsrI	ACTGG	2 cut(s)	914, 1966
BssAI	RCCGGY	2 cut(s)	33, 645
BssECI	CCNNGG	3 cut(s)	251, 1176, 1838
BssMI	GATC	5 cut(s)	299, 918, 943, 1053, 1208
BssNI	GRCGYC	2 cut(s)	435, 633
BssSI	CACGAG	3 cut(s)	209, 1236, 1657
BssT1I	CCWWGG	1 cut(s)	1838
Bst2BI	CACGAG	3 cut(s)	209, 1236, 1657
Bst2UI	CCWGG	3 cut(s)	490, 1025, 1796
Bst4CI	ACNGT	2 cut(s)	709, 1124
Bst6I	CTCTTC	2 cut(s)	1261, 1526
BstACI	GRCGYC	2 cut(s)	435, 633
BstAPI	GCANNNNNTGC	2 cut(s)	1009, 1736
BstAUI	TGTACA	1 cut(s)	97
BstBI	TTCGAA	1 cut(s)	771
BstC8I	GCNNGC	4 cut(s)	35, 647, 1254, 1974
BstDEI	CTNAG	9 cut(s)	142, 227, 837, 1146, 1451, 1470, 1722, 1905, 2080
BstDSI	CCRYGG	2 cut(s)	251, 1176
BstEII	GGTNACC	2 cut(s)	42, 115
BstF5I	GGATG	6 cut(s)	361, 436, 1188, 1210, 1615, 1824
BstFNI	CGCG	2 cut(s)	1178, 1278
BstH2I	RGCGCY	1 cut(s)	1691
BstHHI	GCGC	5 cut(s)	33, 326, 1220, 1280, 1690
BstKTI	GATC	5 cut(s)	302, 921, 946, 1056, 1211
BstMAI	GTCTC	5 cut(s)	115, 624, 845, 1335, 2054
BstMBI	GATC	5 cut(s)	299, 918, 943, 1053, 1208
BstMCI	CGRYCG	2 cut(s)	591, 1176
BstNI	CCWGG	3 cut(s)	490, 1025, 1796
BstPAI	GACNNNNGTC	2 cut(s)	107, 260
BstPI	GGTNACC	2 cut(s)	42, 115
BstSCI	CCNGG	5 cut(s)	326, 488, 573, 1023, 1794
BstSFI	CTRYAG	5 cut(s)	476, 1227, 1488, 1512, 2028
BstSLI	GKGCMC	2 cut(s)	1070, 1464
BstUI	CGCG	2 cut(s)	1178, 1278
BstV2I	GAAGAC	1 cut(s)	783
BstX2I	RGATCY	1 cut(s)	299
BstXI	CCANNNNNNTGG	1 cut(s)	895
BstYI	RGATCY	1 cut(s)	299
BstZI	CGGCCG	1 cut(s)	588
Bsu15I	ATCGAT	1 cut(s)	26
BsuRI	GGCC	6 cut(s)	92, 375, 488, 573, 590, 1346
BsuTUI	ATCGAT	1 cut(s)	26
BtgI	CCRYGG	2 cut(s)	251, 1176
BtgZI	GCGATG	1 cut(s)	512
BtrI	CACGTC	3 cut(s)	88, 418, 454
BtsCI	GGATG	6 cut(s)	361, 436, 1188, 1210, 1615, 1824
BtsI	GCAGTG	2 cut(s)	1067, 1806
BtsIMutI	CAGTG	4 cut(s)	921, 1067, 1702, 1806
BveI	ACCTGC	3 cut(s)	741, 1796, 2063
Cac8I	GCNNGC	4 cut(s)	35, 647, 1254, 1974
CaiI	CAGNNNCTG	1 cut(s)	1103
CciI	TCATGA	1 cut(s)	1519
CfoI	GCGC	5 cut(s)	33, 326, 1220, 1280, 1690
Cfr10I	RCCGGY	2 cut(s)	33, 645
Cfr13I	GGNCC	5 cut(s)	91, 571, 1345, 1434, 1792
Cfr42I	CCGCGG	1 cut(s)	1179
ClaI	ATCGAT	1 cut(s)	26
CseI	GACGC	1 cut(s)	641
Csp6I	GTAC	5 cut(s)	98, 984, 1590, 1965, 2116
CspCI	CAANNNNNGTGG	4 cut(s)	1323, 1358, 1761, 1796
CviAII	CATG	3 cut(s)	1057, 1215, 1520
CviQI	GTAC	5 cut(s)	98, 984, 1590, 1965, 2116
DdeI	CTNAG	9 cut(s)	142, 227, 837, 1146, 1451, 1470, 1722, 1905, 2080
DpnI	GATC	5 cut(s)	301, 920, 945, 1055, 1210
DpnII	GATC	5 cut(s)	299, 918, 943, 1053, 1208
EaeI	YGGCCR	2 cut(s)	373, 588
EagI	CGGCCG	1 cut(s)	588
Eam1104I	CTCTTC	2 cut(s)	1261, 1526
EarI	CTCTTC	2 cut(s)	1261, 1526
EciI	GGCGGA	2 cut(s)	52, 184
Ecl136II	GAGCTC	2 cut(s)	280, 472
EclXI	CGGCCG	1 cut(s)	588
Eco130I	CCWWGG	1 cut(s)	1838
Eco24I	GRGCYC	3 cut(s)	282, 474, 705
Eco31I	GGTCTC	1 cut(s)	2054
Eco47I	GGWCC	2 cut(s)	1434, 1792
Eco47III	AGCGCT	1 cut(s)	1689
Eco52I	CGGCCG	1 cut(s)	588
Eco53kI	GAGCTC	2 cut(s)	280, 472
Eco57I	CTGAAG	2 cut(s)	338, 1928
Eco88I	CYCGRG	1 cut(s)	1871
Eco91I	GGTNACC	2 cut(s)	42, 115
EcoICRI	GAGCTC	2 cut(s)	280, 472
EcoO109I	RGGNCCY	1 cut(s)	1792
EcoO65I	GGTNACC	2 cut(s)	42, 115
EcoRI	GAATTC	4 cut(s)	172, 560, 1080, 1354
EcoRII	CCWGG	3 cut(s)	488, 1023, 1794
EcoT14I	CCWWGG	1 cut(s)	1838
EcoT22I	ATGCAT	2 cut(s)	1114, 2040
EcoT38I	GRGCYC	3 cut(s)	282, 474, 705
ErhI	CCWWGG	1 cut(s)	1838
Esp3I	CGTCTC	2 cut(s)	624, 1335
FaeI	CATG	3 cut(s)	1060, 1218, 1523
FaqI	GGGAC	3 cut(s)	245, 1846, 2125
FatI	CATG	3 cut(s)	1056, 1214, 1519
FauI	CCCGC	3 cut(s)	1171, 1823, 1965
FauNDI	CATATG	2 cut(s)	940, 1108
FbaI	TGATCA	2 cut(s)	918, 1053
FblI	GTMKAC	1 cut(s)	1637
FokI	GGATG	6 cut(s)	348, 443, 1175, 1217, 1622, 1831
FriOI	GRGCYC	3 cut(s)	282, 474, 705
FspAI	RTGCGCAY	1 cut(s)	1219
FspBI	CTAG	1 cut(s)	2001
FspI	TGCGCA	1 cut(s)	1219
GlaI	GCGC	5 cut(s)	32, 325, 1219, 1279, 1689
GsaI	CCCAGC	3 cut(s)	1017, 1299, 1677
GsuI	CTGGAG	3 cut(s)	74, 1080, 1220
HaeII	RGCGCY	1 cut(s)	1691
HaeIII	GGCC	6 cut(s)	92, 375, 488, 573, 590, 1346
HapII	CCGG	6 cut(s)	34, 71, 328, 372, 574, 646
HgaI	GACGC	1 cut(s)	641
HhaI	GCGC	5 cut(s)	33, 326, 1220, 1280, 1690
Hin1I	GRCGYC	2 cut(s)	435, 633
Hin1II	CATG	3 cut(s)	1060, 1218, 1523
Hin6I	GCGC	5 cut(s)	31, 324, 1218, 1278, 1688
HinP1I	GCGC	5 cut(s)	31, 324, 1218, 1278, 1688
HincII	GTYRAC	1 cut(s)	76
HindII	GTYRAC	1 cut(s)	76
HindIII	AAGCTT	1 cut(s)	1563
HinfI	GANTC	9 cut(s)	391, 584, 625, 865, 1033, 1100, 1557, 1834, 2096
HpaII	CCGG	6 cut(s)	34, 71, 328, 372, 574, 646
HphI	GGTGA	4 cut(s)	36, 109, 704, 1203
Hpy166II	GTNNAC	5 cut(s)	76, 255, 682, 1068, 1638
Hpy8I	GTNNAC	5 cut(s)	76, 255, 682, 1068, 1638
Hpy99I	CGWCG	1 cut(s)	440
HpyCH4III	ACNGT	2 cut(s)	709, 1124
HpyCH4IV	ACGT	8 cut(s)	87, 417, 435, 453, 696, 1949, 2114, 2118
HpyF3I	CTNAG	9 cut(s)	142, 227, 837, 1146, 1451, 1470, 1722, 1905, 2080
HpySE526I	ACGT	8 cut(s)	87, 417, 435, 453, 696, 1949, 2114, 2118
Hsp92I	GRCGYC	2 cut(s)	435, 633
Hsp92II	CATG	3 cut(s)	1060, 1218, 1523
HspAI	GCGC	5 cut(s)	31, 324, 1218, 1278, 1688
KpnI	GGTACC	1 cut(s)	1593
KroI	GCCGGC	2 cut(s)	33, 645
KroNI	GCCGGC	2 cut(s)	35, 647
Ksp22I	TGATCA	2 cut(s)	918, 1053
KspI	CCGCGG	1 cut(s)	1179
Kzo9I	GATC	5 cut(s)	299, 918, 943, 1053, 1208
LmnI	GCTCC	3 cut(s)	73, 477, 670
LweI	GCATC	5 cut(s)	18, 489, 1121, 1600, 1812
MaeI	CTAG	1 cut(s)	2001
MaeII	ACGT	8 cut(s)	87, 417, 435, 453, 696, 1949, 2114, 2118
MaeIII	GTNAC	9 cut(s)	42, 115, 692, 1320, 1360, 1615, 1681, 1938, 2075
MalI	GATC	5 cut(s)	301, 920, 945, 1055, 1210
MboI	GATC	5 cut(s)	299, 918, 943, 1053, 1208
MboII	GAAGA	9 cut(s)	524, 536, 785, 788, 983, 1278, 1430, 1543, 1992
MflI	RGATCY	1 cut(s)	299
MhlI	GDGCHC	6 cut(s)	216, 282, 474, 705, 1070, 1464
MlyI	GAGTC	3 cut(s)	578, 1109, 1828
MmeI	TCCRAC	1 cut(s)	1216
Mph1103I	ATGCAT	2 cut(s)	1114, 2040
MreI	CGCCGGCG	1 cut(s)	33
MroNI	GCCGGC	2 cut(s)	33, 645
MroXI	GAANNNNTTC	1 cut(s)	204
MseI	TTAA	4 cut(s)	530, 720, 1443, 1917
MslI	CAYNNNNRTG	3 cut(s)	690, 1661, 2043
MspA1I	CMGCKG	5 cut(s)	166, 1133, 1178, 1733, 2084
MspI	CCGG	6 cut(s)	34, 71, 328, 372, 574, 646
MspR9I	CCNGG	5 cut(s)	328, 490, 575, 1025, 1796
Mva1269I	GAATGC	3 cut(s)	734, 1489, 2029
MvaI	CCWGG	3 cut(s)	490, 1025, 1796
MvnI	CGCG	2 cut(s)	1178, 1278
NaeI	GCCGGC	2 cut(s)	35, 647
NciI	CCSGG	2 cut(s)	328, 575
NdeI	CATATG	2 cut(s)	940, 1108
NdeII	GATC	5 cut(s)	299, 918, 943, 1053, 1208
NgoMIV	GCCGGC	2 cut(s)	33, 645
NlaIII	CATG	3 cut(s)	1060, 1218, 1523
NlaIV	GGNNCC	5 cut(s)	579, 1029, 1435, 1591, 1923
NmeAIII	GCCGAG	3 cut(s)	148, 566, 2031
NmuCI	GTSAC	5 cut(s)	42, 115, 692, 1360, 1615
NsbI	TGCGCA	1 cut(s)	1219
NsiI	ATGCAT	2 cut(s)	1114, 2040
NspV	TTCGAA	1 cut(s)	771
OliI	CACNNNNGTG	1 cut(s)	690
PaeR7I	CTCGAG	1 cut(s)	1871
PagI	TCATGA	1 cut(s)	1519
PcsI	WCGNNNNNNNCGW	4 cut(s)	693, 777, 1243, 1472
PctI	GAATGC	3 cut(s)	734, 1489, 2029
PdiI	GCCGGC	2 cut(s)	35, 647
PdmI	GAANNNNTTC	1 cut(s)	204
PfeI	GAWTC	6 cut(s)	391, 625, 865, 1033, 1557, 2096
Pfl23II	CGTACG	1 cut(s)	2115
PflMI	CCANNNNNTGG	1 cut(s)	1198
PleI	GAGTC	3 cut(s)	578, 1108, 1828
PpsI	GAGTC	3 cut(s)	578, 1108, 1828
PpuMI	RGGWCCY	1 cut(s)	1792
PshAI	GACNNNNGTC	2 cut(s)	107, 260
PsiI	TTATAA	1 cut(s)	860
Psp124BI	GAGCTC	2 cut(s)	282, 474
Psp5II	RGGWCCY	1 cut(s)	1792
Psp6I	CCWGG	3 cut(s)	488, 1023, 1794
PspEI	GGTNACC	2 cut(s)	42, 115
PspFI	CCCAGC	3 cut(s)	1013, 1295, 1673
PspGI	CCWGG	3 cut(s)	488, 1023, 1794
PspLI	CGTACG	1 cut(s)	2115
PspN4I	GGNNCC	5 cut(s)	579, 1029, 1435, 1591, 1923
PspPI	GGNCC	5 cut(s)	91, 571, 1345, 1434, 1792
PspPPI	RGGWCCY	1 cut(s)	1792
PspXI	VCTCGAGB	1 cut(s)	1871
PstI	CTGCAG	4 cut(s)	480, 1492, 1516, 2032
PstNI	CAGNNNCTG	1 cut(s)	1103
PsuI	RGATCY	1 cut(s)	299
PvuII	CAGCTG	3 cut(s)	1133, 1733, 2084
RsaI	GTAC	5 cut(s)	99, 985, 1591, 1966, 2117
RsaNI	GTAC	5 cut(s)	98, 984, 1590, 1965, 2116
RseI	CAYNNNNRTG	3 cut(s)	690, 1661, 2043
SacI	GAGCTC	2 cut(s)	282, 474
SacII	CCGCGG	1 cut(s)	1179
SaqAI	TTAA	4 cut(s)	530, 720, 1443, 1917
Sau3AI	GATC	5 cut(s)	299, 918, 943, 1053, 1208
Sau96I	GGNCC	5 cut(s)	91, 571, 1345, 1434, 1792
SchI	GAGTC	3 cut(s)	578, 1109, 1828
ScrFI	CCNGG	5 cut(s)	328, 490, 575, 1025, 1796
SduI	GDGCHC	6 cut(s)	216, 282, 474, 705, 1070, 1464
SfaNI	GCATC	5 cut(s)	18, 489, 1121, 1600, 1812
SfcI	CTRYAG	5 cut(s)	476, 1227, 1488, 1512, 2028
Sfr274I	CTCGAG	1 cut(s)	1871
Sfr303I	CCGCGG	1 cut(s)	1179
SfuI	TTCGAA	1 cut(s)	771
SgrAI	CRCCGGYG	1 cut(s)	33
SgrBI	CCGCGG	1 cut(s)	1179
SinI	GGWCC	2 cut(s)	1434, 1792
SlaI	CTCGAG	1 cut(s)	1871
SmiMI	CAYNNNNRTG	3 cut(s)	690, 1661, 2043
SmlI	CTYRAG	1 cut(s)	1871
SmoI	CTYRAG	1 cut(s)	1871
SspMI	CTAG	1 cut(s)	2001
SstI	GAGCTC	2 cut(s)	282, 474
StyD4I	CCNGG	5 cut(s)	326, 488, 573, 1023, 1794
StyI	CCWWGG	1 cut(s)	1838
TaaI	ACNGT	2 cut(s)	709, 1124
TaiI	ACGT	8 cut(s)	90, 420, 438, 456, 699, 1952, 2117, 2121
TaqII	GACCGA	1 cut(s)	103
TatI	WGTACW	2 cut(s)	97, 983
TauI	GCSGC	2 cut(s)	14, 169
TfiI	GAWTC	6 cut(s)	391, 625, 865, 1033, 1557, 2096
Tru1I	TTAA	4 cut(s)	530, 720, 1443, 1917
Tru9I	TTAA	4 cut(s)	530, 720, 1443, 1917
TscAI	CASTG	4 cut(s)	921, 1067, 1702, 1806
TseFI	GTSAC	5 cut(s)	42, 115, 692, 1360, 1615
Tsp45I	GTSAC	5 cut(s)	42, 115, 692, 1360, 1615
TspDTI	ATGAA	4 cut(s)	496, 537, 1273, 1508
TspRI	CASTG	4 cut(s)	921, 1067, 1702, 1806
Van91I	CCANNNNNTGG	1 cut(s)	1198
VneI	GTGCAC	1 cut(s)	1066
VpaK11BI	GGWCC	2 cut(s)	1434, 1792
XapI	RAATTY	7 cut(s)	172, 399, 560, 1048, 1080, 1242, 1354
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1021
XhoI	CTCGAG	1 cut(s)	1871
XmiI	GTMKAC	1 cut(s)	1637
XmnI	GAANNNNTTC	1 cut(s)	204
XspI	CTAG	1 cut(s)	2001
ZraI	GACGTC	1 cut(s)	436
Zsp2I	ATGCAT	2 cut(s)	1114, 2040

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.