Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr7

Orthogroup ID

OG0014286

Physical Location & Seq

Genomic Coordinates Forward (+)

8912584 .. 8914734

Gene Structure 2151 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000031870

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2151 bp

ATGGCTTCCGCGGCGATATTCTCTTCGTTACGGCGGCGGAGGTCGCCGTCTCTGGAGGCGTTTTTAGCTCCGGTTGACCTCTCCGACGTGGCGCTTGTACAGACTCTGGTTTCGGTGACGGCGGAGATTGTGTCGTCGTTTTCAGACAAGTTGTTCTATTTCCAGAGACGGAATTCGAGGTCGTTGGTCCGGAAAGTGGAGGTCTTTCTCGTGCTTTTGGAGTCTTTGAACGACTCCAGCGGGTCAACTCTGCCGTGGACGGCGGTGATTTGCCTAAAGGAGCTCTATCTGCTGCTGTACAGATCCAAGATTCTCCTCGATTACTGCGCGCAGTCCAGTAAGCTCTGGCTTTTGCTCCAGCACCATCCGATTTCCGGCCATTTCCACGACTTGAATCAGGAAATCTCGACGCTTTTGGATGTTTTTCCGATTGACGAGGTTGAGTTGGGGGAGGACGTGAGGGAGCAGATTGAGCTTCTGCAGAGACAGGCGAGGAGGGCGAGGCTGTTGATCGACAAAGCCGACGAGGAGTTGAGGATTCAGTTGTTTAGTTTCCTACATGAATTCGACAAGGGAAGGGTGCCCAATTCGGATGACGTGTGGGTGTTTTTCCATCAGAGATTGGGGATTAGGGATGCCAAGAGCTGCAGACATGAGATTGAGTTCTTGGAAGAGCAGATTGTGAATCACGAGGGGGATGTTGAGCCTACAGTGTCTGTGCTTAATGGCTTTGTGGCCTTTACAAGGTATTGCAGGTTCTTGCTTTTCGGATTCGAGGAGGATGAAGTGGGAGTTGGGAGTCAGAGGAAGCCGAGGAAGGGTCTGATTAGTCAGGAGATTGCTGAGACTTTTATTACCATTCCCAAGGATTTTTGCTGCCCTATTAGTTTGGATTTGATGAGAGAGCCTGTGATAATCTCCACCGGGCAGACGTATGATCGGATTTCGATTTCGAGGTGGCTGGAAGAGGGGCATTGTACTTGTCCGAAAACAGGTCAAATGCTGACCAATACGAGGCTTGTTCCCAATCGGGCGTTGAGGAATTTGATCTTGCAGTGGTGCACTGCTTATGGAGTTCCTTATGATGCTCCCGAGTGTACTGAAGCATCAGCAGAAAGCTTTGCAGGGGCTTCTCCCTCTAAGGCTGCACTTGCAGCCAACAGAGCTACAGCGGGGCTTTTGATCAAACGGCTAGCGGAGGATGGATCGACATGCGGACACACTATAGCTGCTCGGGAGATTCGGTTGTTGGCCAAGACTGGAAGAGAGAACCGTGCTTTCATTGCGGAAGCTGGGGCAATTCCCCATCTTCGGAAGCTGCTTTCTTCTCCAAACTCGGTTGCACAAGAGAACTCTGTGACGGCAATGCTTAACCTGTCGATTTATGACAAAAACAAAAGCAGGATTATGGATGAAGAAGGCTGTTTGGGAGCGATTGTCCATGTGCTGAGGTTTGGGCACACAACAGAAGCAAGGGAGAATGCCGCGGCAACGTTGTTTAGTCTGTCTGCAGTTCATGACTACAAGAAGAGAATAGCGGATGAGGAAGGGGGAATTGAAGCCTTGGCAGGGCTGTTGTTACATGGCACCCAAAGAGGAAAGAAGGATGCTGTGACAGCTTTGTTTAACTTGTCCACGCACACAGAAAATTGCGCGAGAATGATTGAGGCTGGGGCTGTAACAGCATTGGTAAGTGCATTGGGAATTGAAGGTGTTGCTGAGGAATCAGCAGGTGCATTGGCCTTGATTGTTAGGCAGCCAAGTGGAGCTACAGCAGTAGGAAAAGAGGAAATGGCAGTGGCTGGTCTAATAGGGATGATGCGTTGCGGGACTCCAAGGGGTAAAGAAAATGCAGTTGCAGCTTTGCTTGAGTTGTGTCGGCATGGTGGAGCTGCTGCAACTGAGAGAGTAGTCAAGGCACCATCTTTGGCTGGTTTGCTTCAGACACTGCTTTTCACCGGTACAAAGAGGGCAAGAAGAAAGGCGGCGTCTCTTGCTAGAGTGTTTCAAAGGTGTGAGAATGCGTCGACAATGCATTATGGTGGTCTCGGAGTACGATACGCATTTGCAGGCAACTCGGCTGCAAATCGTGATACAAGTTTCGTTGGTGATGTCTCAGTGCAGATGTCCATTTCGGTGCCTGTGTTATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002376 GO:0003674 GO:0003824 GO:0004842 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0006464 GO:0006807 GO:0006950 GO:0006952 GO:0006955 GO:0008150 GO:0008152 GO:0009605 GO:0009607 GO:0009814 GO:0009987 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0032446 GO:0036211 GO:0043170 GO:0043207 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044424 GO:0044464 GO:0045087 GO:0050896 GO:0051704 GO:0051707 GO:0070647 GO:0071704 GO:0098542 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Arm U-box

Protein Analysis

717

Amino Acids

78.52

Weight (kDa)

6.82

Isoelectric Point (pI)

46.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PUB10_N	PF25368	56 - 167	5.3e-14	PUB10-like, N-terminal domain
U-box	PF04564	287 - 354	5.8e-19	U-box domain
ARM_PUB	PF25598	395 - 671	3.6e-17	Plant U-box protein ARM repeat region
Arm	PF00514	423 - 460	1e-07	Armadillo/beta-catenin-like repeat

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Orthologous Genes (Group: OG0014286)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G29340
fragaria_vesca	FvH4_3g07220
malus_domestica	MD00G1008100.v1.1 MD10G1274600.v1.1
prunus_persica	Prupe.4G067800_v2.0.a1
pyrus_communis	pycom05g27330 pycom10g22910
rosa_chinensis	RchiOBHm_Chr5g0011931
rosa_laevigata	RLG00000031870
rosa_multiflora	Rmu_ssc0000228.1_g000008
rosa_roxburghii	Rroxscaffold_1G00064660
rosa_rugosa	Rorug04G0460100
rosa_samantha	Rh5AG091600 Rh5BG087400 Rh5CG101300 Rh5DG088000
rosa_wichuraiana	Rw5G007990

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1721
Acc36I	ACCTGC	2 cut(s)	744, 1721
AccB1I	GGYRCC	4 cut(s)	580, 1586, 1918, 2137
AccI	GTMKAC	1 cut(s)	2027
AccII	CGCG	4 cut(s)	11, 329, 1487, 1655
AccIII	TCCGGA	1 cut(s)	189
AclI	AACGTT	1 cut(s)	1493
AclWI	GGATC	2 cut(s)	297, 1213
AcoI	YGGCCR	2 cut(s)	376, 1251
AcsI	RAATTY	3 cut(s)	172, 563, 1042
AcuI	CTGAAG	2 cut(s)	1122, 1925
AcyI	GRCGYC	1 cut(s)	1988
AfaI	GTAC	6 cut(s)	99, 299, 979, 1099, 1963, 2055
AfiI	CCNNNNNNNGG	8 cut(s)	196, 374, 744, 818, 992, 1014, 1311, 1569
AflIII	ACRYGT	1 cut(s)	597
AgeI	ACCGGT	1 cut(s)	1958
AgsI	TTSAA	5 cut(s)	229, 394, 1559, 1709, 2009
AjiI	CACGTC	3 cut(s)	88, 457, 598
AjuI	GAANNNNNNNTTGG	2 cut(s)	777, 809
Alw21I	GWGCWC	2 cut(s)	285, 1064
Alw26I	GTCTC	7 cut(s)	54, 160, 478, 839, 1995, 2051, 2119
Alw44I	GTGCAC	1 cut(s)	1060
AlwI	GGATC	2 cut(s)	297, 1213
AlwNI	CAGNNNCTG	4 cut(s)	106, 716, 1802, 1948
Ama87I	CYCGRG	2 cut(s)	1091, 1233
Aor13HI	TCCGGA	1 cut(s)	189
AoxI	GGCC	4 cut(s)	376, 735, 1251, 1740
ApaLI	GTGCAC	1 cut(s)	1060
ApoI	RAATTY	3 cut(s)	172, 563, 1042
ArsI	GACNNNNNNTTYG	2 cut(s)	94, 126
AsiGI	ACCGGT	1 cut(s)	1958
AspLEI	GCGC	4 cut(s)	94, 329, 331, 1655
AspS9I	GGNCC	1 cut(s)	187
AsuC2I	CCSGG	1 cut(s)	925
AsuHPI	GGTGA	4 cut(s)	127, 277, 1948, 2120
AsuNHI	GCTAGC	1 cut(s)	1192
AvaI	CYCGRG	2 cut(s)	1091, 1233
AvaII	GGWCC	1 cut(s)	187
BaeGI	GKGCMC	3 cut(s)	585, 1064, 1461
BalI	TGGCCA	1 cut(s)	1253
BanI	GGYRCC	4 cut(s)	580, 1586, 1918, 2137
BanII	GRGCYC	1 cut(s)	285
BauI	CACGAG	2 cut(s)	209, 689
Bbv12I	GWGCWC	2 cut(s)	285, 1064
BbvCI	CCTCAGC	2 cut(s)	1448, 1719
BccI	CCATC	5 cut(s)	372, 621, 1196, 1314, 1930
BceAI	ACGGC	7 cut(s)	31, 47, 135, 238, 276, 1205, 1377
BcgI	CGANNNNNNTGC	2 cut(s)	2058, 2092
BclI	TGATCA	1 cut(s)	1182
BcnI	CCSGG	1 cut(s)	925
BcoDI	GTCTC	7 cut(s)	54, 160, 478, 839, 1995, 2051, 2119
BfaI	CTAG	2 cut(s)	1193, 1998
BfmI	CTRYAG	7 cut(s)	479, 646, 708, 1167, 1224, 1509, 1770
BfoI	RGCGCY	1 cut(s)	95
BfuAI	ACCTGC	2 cut(s)	744, 1721
Bme1390I	CCNGG	1 cut(s)	925
Bme18I	GGWCC	1 cut(s)	187
BmeT110I	CYCGRG	2 cut(s)	1091, 1233
BmgBI	CACGTC	3 cut(s)	88, 457, 598
BmgT120I	GGNCC	1 cut(s)	187
BmiI	GGNNCC	4 cut(s)	582, 1588, 1920, 2139
BmrFI	CCNGG	1 cut(s)	925
BmsI	GCATC	5 cut(s)	625, 1075, 1115, 1597, 1809
BmtI	GCTAGC	1 cut(s)	1196
BpmI	CTGGAG	3 cut(s)	74, 220, 341
Bpu10I	CCTNAGC	2 cut(s)	1448, 1719
BpuEI	CTTGAG	1 cut(s)	1889
BpuMI	CCSGG	1 cut(s)	925
BsaHI	GRCGYC	1 cut(s)	1988
BsaI	GGTCTC	1 cut(s)	2051
BsaJI	CCNNGG	7 cut(s)	9, 254, 812, 864, 1485, 1563, 1835
BsaWI	WCCGGW	3 cut(s)	70, 189, 1958
BsaXI	ACNNNNNCTCC	6 cut(s)	116, 146, 1229, 1259, 1422, 1452
Bsc4I	CCNNNNNNNGG	8 cut(s)	196, 374, 744, 818, 992, 1014, 1311, 1569
Bse118I	RCCGGY	1 cut(s)	1958
Bse1I	ACTGG	2 cut(s)	336, 1264
Bse3DI	GCAATG	2 cut(s)	1281, 1371
BseAI	TCCGGA	1 cut(s)	189
BseDI	CCNNGG	7 cut(s)	9, 254, 812, 864, 1485, 1563, 1835
BseLI	CCNNNNNNNGG	8 cut(s)	196, 374, 744, 818, 992, 1014, 1311, 1569
BseMI	GCAATG	2 cut(s)	1281, 1371
BseMII	CTCAG	5 cut(s)	834, 1439, 1710, 1893, 2130
BseNI	ACTGG	2 cut(s)	336, 1264
BsePI	GCGCGC	1 cut(s)	327
BseRI	GAGGAG	4 cut(s)	305, 508, 542, 791
BseSI	GKGCMC	3 cut(s)	585, 1064, 1461
BseYI	CCCAGC	2 cut(s)	1292, 1670
BsgI	GTGCAG	2 cut(s)	1131, 2141
Bsh1236I	CGCG	4 cut(s)	11, 329, 1487, 1655
BshFI	GGCC	4 cut(s)	378, 737, 1253, 1742
BshNI	GGYRCC	4 cut(s)	580, 1586, 1918, 2137
BshTI	ACCGGT	1 cut(s)	1958
BsiHKAI	GWGCWC	2 cut(s)	285, 1064
BsiHKCI	CYCGRG	2 cut(s)	1091, 1233
BsiSI	CCGG	5 cut(s)	71, 190, 375, 924, 1959
BslFI	GGGAC	1 cut(s)	1843
BslI	CCNNNNNNNGG	8 cut(s)	196, 374, 744, 818, 992, 1014, 1311, 1569
BsmAI	GTCTC	7 cut(s)	54, 160, 478, 839, 1995, 2051, 2119
BsmBI	CGTCTC	3 cut(s)	54, 160, 1995
BsmFI	GGGAC	1 cut(s)	1843
BsmI	GAATGC	2 cut(s)	1486, 2026
BsnI	GGCC	4 cut(s)	378, 737, 1253, 1742
Bso31I	GGTCTC	1 cut(s)	2051
BsoBI	CYCGRG	2 cut(s)	1091, 1233
Bsp1286I	GDGCHC	4 cut(s)	285, 585, 1064, 1461
Bsp13I	TCCGGA	1 cut(s)	189
Bsp1407I	TGTACA	2 cut(s)	97, 297
Bsp143I	GATC	6 cut(s)	302, 510, 937, 1047, 1182, 1205
BspANI	GGCC	4 cut(s)	378, 737, 1253, 1742
BspCNI	CTCAG	5 cut(s)	835, 1440, 1711, 1894, 2129
BspEI	TCCGGA	1 cut(s)	189
BspFNI	CGCG	4 cut(s)	11, 329, 1487, 1655
BspHI	TCATGA	1 cut(s)	1516
BspLI	GGNNCC	4 cut(s)	582, 1588, 1920, 2139
BspMAI	CTGCAG	3 cut(s)	483, 650, 1513
BspMI	ACCTGC	2 cut(s)	744, 1721
BspOI	GCTAGC	1 cut(s)	1196
BspPI	GGATC	2 cut(s)	297, 1213
BspQI	GCTCTTC	1 cut(s)	666
BspT107I	GGYRCC	4 cut(s)	580, 1586, 1918, 2137
BspTNI	GGTCTC	1 cut(s)	2051
BsrDI	GCAATG	2 cut(s)	1281, 1371
BsrFI	RCCGGY	1 cut(s)	1958
BsrGI	TGTACA	2 cut(s)	97, 297
BsrI	ACTGG	2 cut(s)	336, 1264
BssAI	RCCGGY	1 cut(s)	1958
BssECI	CCNNGG	7 cut(s)	9, 254, 812, 864, 1485, 1563, 1835
BssHII	GCGCGC	1 cut(s)	327
BssMI	GATC	6 cut(s)	302, 510, 937, 1047, 1182, 1205
BssNI	GRCGYC	1 cut(s)	1988
BssSI	CACGAG	2 cut(s)	209, 689
BssT1I	CCWWGG	3 cut(s)	864, 1563, 1835
Bst2BI	CACGAG	2 cut(s)	209, 689
Bst4CI	ACNGT	2 cut(s)	712, 1274
Bst6I	CTCTTC	5 cut(s)	28, 666, 960, 1258, 1523
BstACI	GRCGYC	1 cut(s)	1988
BstAUI	TGTACA	2 cut(s)	97, 297
BstC8I	GCNNGC	3 cut(s)	329, 1194, 2071
BstDEI	CTNAG	6 cut(s)	843, 1140, 1448, 1719, 1902, 2116
BstDSI	CCRYGG	3 cut(s)	9, 254, 1485
BstENI	CCTNNNNNAGG	1 cut(s)	742
BstFNI	CGCG	4 cut(s)	11, 329, 1487, 1655
BstH2I	RGCGCY	1 cut(s)	95
BstHHI	GCGC	4 cut(s)	94, 329, 331, 1655
BstKTI	GATC	6 cut(s)	305, 513, 940, 1050, 1185, 1208
BstMAI	GTCTC	7 cut(s)	54, 160, 478, 839, 1995, 2051, 2119
BstMBI	GATC	6 cut(s)	302, 510, 937, 1047, 1182, 1205
BstNSI	RCATGY	1 cut(s)	1215
BstSCI	CCNGG	1 cut(s)	923
BstSFI	CTRYAG	7 cut(s)	479, 646, 708, 1167, 1224, 1509, 1770
BstSLI	GKGCMC	3 cut(s)	585, 1064, 1461
BstUI	CGCG	4 cut(s)	11, 329, 1487, 1655
BstX2I	RGATCY	1 cut(s)	302
BstYI	RGATCY	1 cut(s)	302
BsuRI	GGCC	4 cut(s)	378, 737, 1253, 1742
BtgI	CCRYGG	3 cut(s)	9, 254, 1485
BtrI	CACGTC	3 cut(s)	88, 457, 598
BtsI	GCAGTG	4 cut(s)	1061, 1062, 1803, 1946
BtsIMutI	CAGTG	6 cut(s)	717, 1061, 1062, 1803, 1946, 2124
BveI	ACCTGC	2 cut(s)	744, 1721
Cac8I	GCNNGC	3 cut(s)	329, 1194, 2071
CaiI	CAGNNNCTG	4 cut(s)	106, 716, 1802, 1948
CciI	TCATGA	1 cut(s)	1516
CfoI	GCGC	4 cut(s)	94, 329, 331, 1655
Cfr10I	RCCGGY	1 cut(s)	1958
Cfr13I	GGNCC	1 cut(s)	187
Cfr42I	CCGCGG	2 cut(s)	12, 1488
CseI	GACGC	3 cut(s)	418, 1977, 2013
Csp6I	GTAC	6 cut(s)	98, 298, 978, 1098, 1962, 2054
CspAI	ACCGGT	1 cut(s)	1958
CviAII	CATG	7 cut(s)	560, 653, 1212, 1442, 1517, 1583, 1883
CviQI	GTAC	6 cut(s)	98, 298, 978, 1098, 1962, 2054
DdeI	CTNAG	6 cut(s)	843, 1140, 1448, 1719, 1902, 2116
DpnI	GATC	6 cut(s)	304, 512, 939, 1049, 1184, 1207
DpnII	GATC	6 cut(s)	302, 510, 937, 1047, 1182, 1205
EaeI	YGGCCR	2 cut(s)	376, 1251
Eam1104I	CTCTTC	5 cut(s)	28, 666, 960, 1258, 1523
EarI	CTCTTC	5 cut(s)	28, 666, 960, 1258, 1523
EciI	GGCGGA	2 cut(s)	52, 137
Ecl136II	GAGCTC	1 cut(s)	283
Eco130I	CCWWGG	3 cut(s)	864, 1563, 1835
Eco24I	GRGCYC	1 cut(s)	285
Eco31I	GGTCTC	1 cut(s)	2051
Eco47I	GGWCC	1 cut(s)	187
Eco53kI	GAGCTC	1 cut(s)	283
Eco57I	CTGAAG	2 cut(s)	1122, 1925
Eco88I	CYCGRG	2 cut(s)	1091, 1233
EcoICRI	GAGCTC	1 cut(s)	283
EcoNI	CCTNNNNNAGG	1 cut(s)	742
EcoRI	GAATTC	2 cut(s)	172, 563
EcoT14I	CCWWGG	3 cut(s)	864, 1563, 1835
EcoT22I	ATGCAT	1 cut(s)	2037
EcoT38I	GRGCYC	1 cut(s)	285
ErhI	CCWWGG	3 cut(s)	864, 1563, 1835
Esp3I	CGTCTC	3 cut(s)	54, 160, 1995
FaeI	CATG	7 cut(s)	563, 656, 1215, 1445, 1520, 1586, 1886
FaqI	GGGAC	1 cut(s)	1843
FatI	CATG	7 cut(s)	559, 652, 1211, 1441, 1516, 1582, 1882
FauI	CCCGC	3 cut(s)	233, 1165, 1820
FbaI	TGATCA	1 cut(s)	1182
FblI	GTMKAC	1 cut(s)	2027
FriOI	GRGCYC	1 cut(s)	285
FspBI	CTAG	2 cut(s)	1193, 1998
GlaI	GCGC	4 cut(s)	93, 328, 330, 1654
GsaI	CCCAGC	2 cut(s)	1296, 1674
GsuI	CTGGAG	3 cut(s)	74, 220, 341
HaeII	RGCGCY	1 cut(s)	95
HaeIII	GGCC	4 cut(s)	378, 737, 1253, 1742
HapII	CCGG	5 cut(s)	71, 190, 375, 924, 1959
HgaI	GACGC	3 cut(s)	418, 1977, 2013
HhaI	GCGC	4 cut(s)	94, 329, 331, 1655
Hin1I	GRCGYC	1 cut(s)	1988
Hin1II	CATG	7 cut(s)	563, 656, 1215, 1445, 1520, 1586, 1886
Hin6I	GCGC	4 cut(s)	92, 327, 329, 1653
HinP1I	GCGC	4 cut(s)	92, 327, 329, 1653
HincII	GTYRAC	3 cut(s)	76, 246, 2028
HindII	GTYRAC	3 cut(s)	76, 246, 2028
HindIII	AAGCTT	1 cut(s)	1117
HpaII	CCGG	5 cut(s)	71, 190, 375, 924, 1959
HphI	GGTGA	4 cut(s)	127, 277, 1948, 2120
Hpy166II	GTNNAC	7 cut(s)	76, 246, 258, 1062, 1098, 1635, 2028
Hpy8I	GTNNAC	7 cut(s)	76, 246, 258, 1062, 1098, 1635, 2028
Hpy99I	CGWCG	5 cut(s)	89, 139, 412, 527, 2029
HpyAV	CCTTC	6 cut(s)	570, 811, 1412, 1541, 1597, 1703
HpyCH4III	ACNGT	2 cut(s)	712, 1274
HpyCH4IV	ACGT	5 cut(s)	87, 456, 597, 932, 1493
HpyF3I	CTNAG	6 cut(s)	843, 1140, 1448, 1719, 1902, 2116
HpySE526I	ACGT	5 cut(s)	87, 456, 597, 932, 1493
Hsp92I	GRCGYC	1 cut(s)	1988
Hsp92II	CATG	7 cut(s)	563, 656, 1215, 1445, 1520, 1586, 1886
HspAI	GCGC	4 cut(s)	92, 327, 329, 1653
Kpn2I	TCCGGA	1 cut(s)	189
Ksp22I	TGATCA	1 cut(s)	1182
KspI	CCGCGG	2 cut(s)	12, 1488
Kzo9I	GATC	6 cut(s)	302, 510, 937, 1047, 1182, 1205
LguI	GCTCTTC	1 cut(s)	666
LmnI	GCTCC	8 cut(s)	73, 280, 360, 463, 1093, 1430, 1766, 1889
LweI	GCATC	5 cut(s)	625, 1075, 1115, 1597, 1809
MaeI	CTAG	2 cut(s)	1193, 1998
MaeII	ACGT	5 cut(s)	87, 456, 597, 932, 1493
MaeIII	GTNAC	6 cut(s)	27, 115, 1357, 1578, 1612, 1678
MalI	GATC	6 cut(s)	304, 512, 939, 1049, 1184, 1207
MboI	GATC	6 cut(s)	302, 510, 937, 1047, 1182, 1205
MboII	GAAGA	9 cut(s)	15, 683, 977, 1275, 1301, 1317, 1427, 1540, 1989
MflI	RGATCY	1 cut(s)	302
MhlI	GDGCHC	4 cut(s)	285, 585, 1064, 1461
MlsI	TGGCCA	1 cut(s)	1253
MluCI	AATT	8 cut(s)	172, 563, 586, 1042, 1299, 1554, 1648, 1704
MluNI	TGGCCA	1 cut(s)	1253
MlyI	GAGTC	5 cut(s)	97, 227, 230, 808, 1825
MmeI	TCCRAC	1 cut(s)	108
Mox20I	TGGCCA	1 cut(s)	1253
Mph1103I	ATGCAT	1 cut(s)	2037
MroI	TCCGGA	1 cut(s)	189
MscI	TGGCCA	1 cut(s)	1253
MseI	TTAA	3 cut(s)	723, 1371, 1626
MslI	CAYNNNNRTG	2 cut(s)	2040, 2135
Msp20I	TGGCCA	1 cut(s)	1253
MspA1I	CMGCKG	4 cut(s)	11, 240, 1172, 1487
MspI	CCGG	5 cut(s)	71, 190, 375, 924, 1959
MspR9I	CCNGG	1 cut(s)	925
Mva1269I	GAATGC	2 cut(s)	1486, 2026
MvnI	CGCG	4 cut(s)	11, 329, 1487, 1655
NciI	CCSGG	1 cut(s)	925
NdeII	GATC	6 cut(s)	302, 510, 937, 1047, 1182, 1205
NheI	GCTAGC	1 cut(s)	1192
NlaIII	CATG	7 cut(s)	563, 656, 1215, 1445, 1520, 1586, 1886
NlaIV	GGNNCC	4 cut(s)	582, 1588, 1920, 2139
NmeAIII	GCCGAG	2 cut(s)	837, 2057
NmuCI	GTSAC	3 cut(s)	115, 1357, 1612
NsiI	ATGCAT	1 cut(s)	2037
NspI	RCATGY	1 cut(s)	1215
PagI	TCATGA	1 cut(s)	1516
PaqCI	CACCTGC	1 cut(s)	1721
PauI	GCGCGC	1 cut(s)	327
PciSI	GCTCTTC	1 cut(s)	666
PcsI	WCGNNNNNNNCGW	1 cut(s)	519
PctI	GAATGC	2 cut(s)	1486, 2026
PfeI	GAWTC	7 cut(s)	310, 394, 538, 685, 771, 1240, 1724
PinAI	ACCGGT	1 cut(s)	1958
PleI	GAGTC	5 cut(s)	97, 227, 229, 807, 1825
PpsI	GAGTC	5 cut(s)	97, 227, 229, 807, 1825
Psp124BI	GAGCTC	1 cut(s)	285
Psp1406I	AACGTT	1 cut(s)	1493
PspFI	CCCAGC	2 cut(s)	1292, 1670
PspN4I	GGNNCC	4 cut(s)	582, 1588, 1920, 2139
PspPI	GGNCC	1 cut(s)	187
PsrI	GAACNNNNNNTAC	2 cut(s)	740, 772
PstI	CTGCAG	3 cut(s)	483, 650, 1513
PstNI	CAGNNNCTG	4 cut(s)	106, 716, 1802, 1948
PsuI	RGATCY	1 cut(s)	302
PteI	GCGCGC	1 cut(s)	327
RsaI	GTAC	6 cut(s)	99, 299, 979, 1099, 1963, 2055
RsaNI	GTAC	6 cut(s)	98, 298, 978, 1098, 1962, 2054
RseI	CAYNNNNRTG	2 cut(s)	2040, 2135
SacI	GAGCTC	1 cut(s)	285
SacII	CCGCGG	2 cut(s)	12, 1488
SalI	GTCGAC	1 cut(s)	2026
SapI	GCTCTTC	1 cut(s)	666
SaqAI	TTAA	3 cut(s)	723, 1371, 1626
Sau3AI	GATC	6 cut(s)	302, 510, 937, 1047, 1182, 1205
Sau96I	GGNCC	1 cut(s)	187
SchI	GAGTC	5 cut(s)	97, 227, 230, 808, 1825
ScrFI	CCNGG	1 cut(s)	925
SduI	GDGCHC	4 cut(s)	285, 585, 1064, 1461
SfaNI	GCATC	5 cut(s)	625, 1075, 1115, 1597, 1809
SfcI	CTRYAG	7 cut(s)	479, 646, 708, 1167, 1224, 1509, 1770
Sfr303I	CCGCGG	2 cut(s)	12, 1488
SgrBI	CCGCGG	2 cut(s)	12, 1488
SinI	GGWCC	1 cut(s)	187
SmiMI	CAYNNNNRTG	2 cut(s)	2040, 2135
SmlI	CTYRAG	1 cut(s)	1868
SmoI	CTYRAG	1 cut(s)	1868
Sse9I	AATT	8 cut(s)	172, 563, 586, 1042, 1299, 1554, 1648, 1704
SspMI	CTAG	2 cut(s)	1193, 1998
SstI	GAGCTC	1 cut(s)	285
StyD4I	CCNGG	1 cut(s)	923
StyI	CCWWGG	3 cut(s)	864, 1563, 1835
TaaI	ACNGT	2 cut(s)	712, 1274
TaiI	ACGT	5 cut(s)	90, 459, 600, 935, 1496
TasI	AATT	8 cut(s)	172, 563, 586, 1042, 1299, 1554, 1648, 1704
TatI	WGTACW	4 cut(s)	97, 297, 977, 1097
TauI	GCSGC	5 cut(s)	14, 37, 1487, 1490, 1988
TfiI	GAWTC	7 cut(s)	310, 394, 538, 685, 771, 1240, 1724
Tru1I	TTAA	3 cut(s)	723, 1371, 1626
Tru9I	TTAA	3 cut(s)	723, 1371, 1626
TscAI	CASTG	6 cut(s)	717, 1061, 1069, 1803, 1953, 2124
TseFI	GTSAC	3 cut(s)	115, 1357, 1612
Tsp45I	GTSAC	3 cut(s)	115, 1357, 1612
TspDTI	ATGAA	5 cut(s)	576, 798, 1270, 1428, 1505
TspGWI	ACGGA	1 cut(s)	184
TspRI	CASTG	6 cut(s)	717, 1061, 1069, 1803, 1953, 2124
VneI	GTGCAC	1 cut(s)	1060
VpaK11BI	GGWCC	1 cut(s)	187
XagI	CCTNNNNNAGG	1 cut(s)	742
XapI	RAATTY	3 cut(s)	172, 563, 1042
XceI	RCATGY	1 cut(s)	1215
XmiI	GTMKAC	1 cut(s)	2027
XspI	CTAG	2 cut(s)	1193, 1998
Zsp2I	ATGCAT	1 cut(s)	2037

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.