Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0009195

Physical Location & Seq

Genomic Coordinates Reverse (-)

2418437 .. 2428657

Gene Structure 10221 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1016800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1467 bp

ATGGCAAAGAAGGTCTTGTTGAAGGAATATGATGAAGAAACTCATGAAGTCTGTCGCAGTGACTTTCCTCCCAACTTCGTTTTTGGCGTTGCCACTTCCGCATATCAAGTAGAGGGAGCCTGCAAGGACGGTGGTAGGGGTCCCAGCATTTGGGATGCTTTTTCACACTCAAAAGGGAATATCATTGATGGAAGCAATGGGGACGTTGCAGTGGATCAATATTATCGGTATAAGGAAGATGTTGAACTCATTGCCAAGTTGGGATTTGAAGCTCATCGTTTTTCCATAGCATGGTCTCGAATATTCCCTGATGGTTTGGGAACCAAAATCAATGAAGAAGGGATTACTTACTATAACAATTTCATCAATGCTCTCCTTGAAAAGGGCATCCAACCTTATGTAACACTGTACCATTGGGATCTACCCTTGTACCTTCATGAGAATATGGGAGGGTGGCTGAATAAGCAAATTGTAGAATACTTCTCAGTCTATGCAGACACCTGCTTTGCAAGCTTTGGCGATAGAGTAAAGGACTGGATCACTTTTAATGAGCCTCTTCAGACTGCAATTAATGGTTATGGTGTTGGGATATTTGCTCCTGGAAGGCACTCGTCAACAGAACCATATTTGGTTGCACACCACCAACTATTGGCACATGCTGCTGCTGTTTCCATATACCGAAGCAAGTATAAGGACAAGCAAGGGGGACAAGTTGGCTTGGTTGTAGACTGTGAATGGGCAGAGGCTAATTCAGACACAATTGAAGATAAAGTTGCTGCAGCAAGGCGCCTAGATTTTCAGCTTGGATGGTACTTAGATCCAATATATCATGGGGAATACCCCAAAGTTATGCGTGAAAGACTTGGAGATCGGCTTCCCATATTTTCAGAGAAAGATAAGGAGTTGCTTAAGAACTCAGTAGACTTTATTGGTCTGAATCACTATACATCAAGATTTATTGCTCATGGAAGAGATAACCATGATGAGGGTGATTTTTATAAAGCACAAGGGATAGAGAGAATTGATGAATGGGAAGGGGGTGAGGCTATTGGTGAGAAGGCAGCATCAGAATGGCTGTATGTTGTTCCTTGGGGCATGCATAAGGTTTTGAATTACATAGCGCAGAGATACAATAATCCCCCAATATATGTTACTGAGAATGGTATGGATGACGAAGATAATGACACCTCCTCACTTCATGAAATGCTGGATGACAAACTGAGAGTTAGTTACTTTAAGAGATACCTTTCAGCAGTTGCCAAAGCAATAAGGGATGGGGCAGATGTAAGGGGATACTTTGCGTGGTCATTACTAGATAACTTTGAGTGGGCTCAAGGATATACCAAGCGTTTTGGTTTGGTTTATGTTGACTATAAGAATGGGCTTGCGAGACACCCAAAATCTTCTGCTTACTGGTTCTTGAGGTTCTTCAAAGGTGTTGAAGGCAAATATGGAAAGGAAGAGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000160 GO:0000302 GO:0002218 GO:0002252 GO:0002253 GO:0002376 GO:0002682 GO:0002684 GO:0003674 GO:0003824 GO:0004553 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0006950 GO:0006952 GO:0006955 GO:0006979 GO:0007154 GO:0007165 GO:0008150 GO:0008152 GO:0008422 GO:0009605 GO:0009607 GO:0009617 GO:0009682 GO:0009719 GO:0009723 GO:0009725 GO:0009755 GO:0009866 GO:0009873 GO:0009987 GO:0009991 GO:0010033 GO:0010035 GO:0010038 GO:0010039 GO:0015926 GO:0016787 GO:0016798 GO:0019748 GO:0023052 GO:0031347 GO:0031349 GO:0031667 GO:0032870 GO:0033554 GO:0034599 GO:0034614 GO:0035556 GO:0035690 GO:0042221 GO:0042493 GO:0042594 GO:0042742 GO:0043207 GO:0044424 GO:0044444 GO:0044464 GO:0045087 GO:0045088 GO:0045089 GO:0048518 GO:0048583 GO:0048584 GO:0050776 GO:0050778 GO:0050789 GO:0050794 GO:0050896 GO:0051704 GO:0051707 GO:0051716 GO:0065007 GO:0070887 GO:0071241 GO:0071248 GO:0071281 GO:0071310 GO:0071369 GO:0071495 GO:0071704 GO:0071731 GO:0071732 GO:0080134 GO:0097366 GO:0098542 GO:1901135 GO:1901657 GO:1901698 GO:1901699 GO:1901700 GO:1901701 GO:1902170 GO:1990641

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

489

Amino Acids

55.93

Weight (kDa)

5.46

Isoelectric Point (pI)

21.51

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	20 - 476	5.1e-168	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009195)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G36890 AT5G36890
fragaria_vesca	FvH4_2g00020 FvH4_2g00020 FvH4_2g00020
malus_domestica	MD00G1016800.v1.1 MD02G1137800.v1.1
prunus_persica	Prupe.6G366100_v2.0.a1 Prupe.6G366200_v2.0.a1
pyrus_communis	pycom02g10910 pycom17g24000
rosa_chinensis	RchiOBHm_Chr6g0249281
rosa_laevigata	RLG00000015597
rosa_multiflora	Rmu_sc0004832.1_g000009
rosa_roxburghii	Rroxscaffold_7G00217940
rosa_rugosa	Rorug05G0489800 Rorug05G0489900
rosa_samantha	Rh5BG535000 Rh6AG000800 Rh6BG009300 Rh6CG000100
rosa_wichuraiana	Rw6G000160

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	999
AarI	CACCTGC	1 cut(s)	509
Acc36I	ACCTGC	1 cut(s)	509
AccB1I	GGYRCC	1 cut(s)	786
AccB7I	CCANNNNNTGG	3 cut(s)	150, 291, 649
AccI	GTMKAC	2 cut(s)	726, 921
AciI	CCGC	1 cut(s)	99
AclWI	GGATC	4 cut(s)	222, 426, 545, 812
AcuI	CTGAAG	1 cut(s)	542
AcyI	GRCGYC	1 cut(s)	787
AfaI	GTAC	3 cut(s)	410, 431, 812
AfiI	CCNNNNNNNGG	5 cut(s)	150, 291, 382, 649, 985
AflII	CTTAAG	1 cut(s)	908
AgsI	TTSAA	8 cut(s)	22, 245, 269, 380, 764, 1111, 1432, 1442
AjnI	CCWGG	1 cut(s)	598
AluBI	AGCT	3 cut(s)	272, 513, 802
AluI	AGCT	3 cut(s)	272, 513, 802
Alw26I	GTCTC	2 cut(s)	300, 1384
AlwI	GGATC	4 cut(s)	222, 426, 545, 812
ApeKI	GCWGC	5 cut(s)	659, 662, 776, 779, 1061
ArsI	GACNNNNNNTTYG	2 cut(s)	488, 520
AseI	ATTAAT	1 cut(s)	570
AspLEI	GCGC	2 cut(s)	789, 1123
AspS9I	GGNCC	1 cut(s)	140
AsuHPI	GGTGA	3 cut(s)	1001, 1052, 1064
AvaII	GGWCC	1 cut(s)	140
BaeI	ACNNNNGTAYC	2 cut(s)	413, 446
BanI	GGYRCC	1 cut(s)	786
BanII	GRGCYC	1 cut(s)	1333
BbvI	GCAGC	5 cut(s)	646, 649, 763, 791, 1073
BccI	CCATC	4 cut(s)	182, 305, 801, 1268
BciT130I	CCWGG	1 cut(s)	600
BciVI	GTATCC	1 cut(s)	1286
BcoDI	GTCTC	2 cut(s)	300, 1384
BfaI	CTAG	2 cut(s)	791, 1313
BfmI	CTRYAG	1 cut(s)	777
BfoI	RGCGCY	1 cut(s)	790
BfrI	CTTAAG	1 cut(s)	908
BfuAI	ACCTGC	1 cut(s)	509
BfuI	GTATCC	1 cut(s)	1286
BisI	GCNGC	5 cut(s)	660, 663, 777, 780, 1062
BlsI	GCNGC	5 cut(s)	661, 664, 778, 781, 1063
Bme1390I	CCNGG	1 cut(s)	600
Bme18I	GGWCC	1 cut(s)	140
BmgT120I	GGNCC	1 cut(s)	140
BmiI	GGNNCC	5 cut(s)	118, 141, 142, 322, 788
BmrFI	CCNGG	1 cut(s)	600
BmsI	GCATC	3 cut(s)	145, 396, 1073
BpuEI	CTTGAG	2 cut(s)	1317, 1441
BsaHI	GRCGYC	1 cut(s)	787
BsaI	GGTCTC	1 cut(s)	300
BsaJI	CCNNGG	1 cut(s)	1088
Bsc4I	CCNNNNNNNGG	5 cut(s)	150, 291, 382, 649, 985
Bse1I	ACTGG	2 cut(s)	539, 1418
Bse3DI	GCAATG	2 cut(s)	202, 249
BseBI	CCWGG	1 cut(s)	600
BseDI	CCNNGG	1 cut(s)	1088
BseGI	GGATG	6 cut(s)	160, 387, 812, 1174, 1216, 1279
BseLI	CCNNNNNNNGG	5 cut(s)	150, 291, 382, 649, 985
BseMI	GCAATG	2 cut(s)	202, 249
BseMII	CTCAG	4 cut(s)	498, 930, 1146, 1211
BseNI	ACTGG	2 cut(s)	539, 1418
BseRI	GAGGAG	1 cut(s)	1180
BseXI	GCAGC	5 cut(s)	646, 649, 763, 791, 1073
BseYI	CCCAGC	1 cut(s)	143
BshNI	GGYRCC	1 cut(s)	786
BslFI	GGGAC	3 cut(s)	126, 215, 720
BslI	CCNNNNNNNGG	5 cut(s)	150, 291, 382, 649, 985
BsmAI	GTCTC	2 cut(s)	300, 1384
BsmFI	GGGAC	3 cut(s)	126, 215, 720
Bso31I	GGTCTC	1 cut(s)	300
Bsp1286I	GDGCHC	1 cut(s)	1333
Bsp143I	GATC	5 cut(s)	214, 418, 537, 817, 868
BspACI	CCGC	1 cut(s)	99
BspCNI	CTCAG	4 cut(s)	497, 929, 1147, 1212
BspHI	TCATGA	3 cut(s)	43, 436, 1198
BspLI	GGNNCC	5 cut(s)	118, 141, 142, 322, 788
BspMAI	CTGCAG	1 cut(s)	781
BspMI	ACCTGC	1 cut(s)	509
BspPI	GGATC	4 cut(s)	222, 426, 545, 812
BspT107I	GGYRCC	1 cut(s)	786
BspTI	CTTAAG	1 cut(s)	908
BspTNI	GGTCTC	1 cut(s)	300
BsrDI	GCAATG	2 cut(s)	202, 249
BsrI	ACTGG	2 cut(s)	539, 1418
BssECI	CCNNGG	1 cut(s)	1088
BssMI	GATC	5 cut(s)	214, 418, 537, 817, 868
BssNI	GRCGYC	1 cut(s)	787
BssT1I	CCWWGG	1 cut(s)	1088
Bst2UI	CCWGG	1 cut(s)	600
Bst4CI	ACNGT	3 cut(s)	131, 408, 731
Bst6I	CTCTTC	3 cut(s)	561, 964, 1455
BstACI	GRCGYC	1 cut(s)	787
BstAFI	CTTAAG	1 cut(s)	908
BstAPI	GCANNNNNTGC	1 cut(s)	659
BstC8I	GCNNGC	4 cut(s)	121, 511, 1097, 1386
BstDEI	CTNAG	5 cut(s)	484, 814, 916, 1155, 1220
BstENI	CCTNNNNNAGG	1 cut(s)	380
BstF5I	GGATG	6 cut(s)	160, 387, 812, 1174, 1216, 1279
BstH2I	RGCGCY	1 cut(s)	790
BstHHI	GCGC	2 cut(s)	789, 1123
BstKTI	GATC	5 cut(s)	217, 421, 540, 820, 871
BstMAI	GTCTC	2 cut(s)	300, 1384
BstMBI	GATC	5 cut(s)	214, 418, 537, 817, 868
BstMWI	GCNNNNNNNGC	4 cut(s)	98, 463, 510, 659
BstNI	CCWGG	1 cut(s)	600
BstNSI	RCATGY	2 cut(s)	659, 1099
BstSCI	CCNGG	1 cut(s)	598
BstSFI	CTRYAG	1 cut(s)	777
BstV1I	GCAGC	5 cut(s)	646, 649, 763, 791, 1073
BstX2I	RGATCY	2 cut(s)	418, 817
BstYI	RGATCY	2 cut(s)	418, 817
BsuI	GTATCC	1 cut(s)	1286
BtsCI	GGATG	6 cut(s)	160, 387, 812, 1174, 1216, 1279
BtsI	GCAGTG	2 cut(s)	64, 216
BtsIMutI	CAGTG	3 cut(s)	64, 216, 404
BveI	ACCTGC	1 cut(s)	509
Cac8I	GCNNGC	4 cut(s)	121, 511, 1097, 1386
CciI	TCATGA	3 cut(s)	43, 436, 1198
CfoI	GCGC	2 cut(s)	789, 1123
Cfr13I	GGNCC	1 cut(s)	140
Csp6I	GTAC	3 cut(s)	409, 430, 811
CspCI	CAANNNNNGTGG	2 cut(s)	112, 147
CviAII	CATG	9 cut(s)	44, 291, 437, 656, 830, 965, 980, 1096, 1199
CviQI	GTAC	3 cut(s)	409, 430, 811
DdeI	CTNAG	5 cut(s)	484, 814, 916, 1155, 1220
DinI	GGCGCC	1 cut(s)	788
DpnI	GATC	5 cut(s)	216, 420, 539, 819, 870
DpnII	GATC	5 cut(s)	214, 418, 537, 817, 868
Eam1104I	CTCTTC	3 cut(s)	561, 964, 1455
EarI	CTCTTC	3 cut(s)	561, 964, 1455
Eco130I	CCWWGG	1 cut(s)	1088
Eco24I	GRGCYC	1 cut(s)	1333
Eco31I	GGTCTC	1 cut(s)	300
Eco47I	GGWCC	1 cut(s)	140
Eco57I	CTGAAG	1 cut(s)	542
EcoNI	CCTNNNNNAGG	1 cut(s)	380
EcoO109I	RGGNCCY	1 cut(s)	140
EcoRII	CCWGG	1 cut(s)	598
EcoT14I	CCWWGG	1 cut(s)	1088
EcoT22I	ATGCAT	1 cut(s)	1101
EcoT38I	GRGCYC	1 cut(s)	1333
EgeI	GGCGCC	1 cut(s)	788
EheI	GGCGCC	1 cut(s)	788
ErhI	CCWWGG	1 cut(s)	1088
FaeI	CATG	9 cut(s)	47, 294, 440, 659, 833, 968, 983, 1099, 1202
FalI	AAGNNNNNCTT	1 cut(s)	31
FaqI	GGGAC	3 cut(s)	126, 215, 720
FatI	CATG	9 cut(s)	43, 290, 436, 655, 829, 964, 979, 1095, 1198
FblI	GTMKAC	2 cut(s)	726, 921
Fnu4HI	GCNGC	5 cut(s)	660, 663, 777, 780, 1062
FokI	GGATG	6 cut(s)	167, 374, 819, 1181, 1223, 1286
FriOI	GRGCYC	1 cut(s)	1333
Fsp4HI	GCNGC	5 cut(s)	660, 663, 777, 780, 1062
FspBI	CTAG	2 cut(s)	791, 1313
GlaI	GCGC	2 cut(s)	788, 1122
GluI	GCNGC	5 cut(s)	660, 663, 777, 780, 1062
GsaI	CCCAGC	1 cut(s)	147
HaeII	RGCGCY	1 cut(s)	790
HhaI	GCGC	2 cut(s)	789, 1123
Hin1I	GRCGYC	1 cut(s)	787
Hin1II	CATG	9 cut(s)	47, 294, 440, 659, 833, 968, 983, 1099, 1202
Hin6I	GCGC	2 cut(s)	787, 1121
HinP1I	GCGC	2 cut(s)	787, 1121
HincII	GTYRAC	2 cut(s)	615, 1369
HindII	GTYRAC	2 cut(s)	615, 1369
HindIII	AAGCTT	1 cut(s)	511
HinfI	GANTC	1 cut(s)	937
HphI	GGTGA	3 cut(s)	1001, 1052, 1064
Hpy166II	GTNNAC	4 cut(s)	615, 727, 922, 1369
Hpy188I	TCNGA	5 cut(s)	561, 754, 889, 936, 1069
Hpy188III	TCNNGA	6 cut(s)	44, 297, 437, 951, 1199, 1420
Hpy8I	GTNNAC	4 cut(s)	615, 727, 922, 1369
HpyAV	CCTTC	8 cut(s)	4, 16, 332, 443, 597, 1028, 1051, 1436
HpyCH4III	ACNGT	3 cut(s)	131, 408, 731
HpyCH4IV	ACGT	1 cut(s)	204
HpyCH4V	TGCA	8 cut(s)	123, 209, 494, 509, 566, 635, 779, 1099
HpyF10VI	GCNNNNNNNGC	4 cut(s)	98, 463, 510, 659
HpyF3I	CTNAG	5 cut(s)	484, 814, 916, 1155, 1220
HpySE526I	ACGT	1 cut(s)	204
Hsp92I	GRCGYC	1 cut(s)	787
Hsp92II	CATG	9 cut(s)	47, 294, 440, 659, 833, 968, 983, 1099, 1202
HspAI	GCGC	2 cut(s)	787, 1121
KasI	GGCGCC	1 cut(s)	786
KflI	GGGWCCC	1 cut(s)	140
Kzo9I	GATC	5 cut(s)	214, 418, 537, 817, 868
LmnI	GCTCC	2 cut(s)	116, 601
LpnPI	CCDG	9 cut(s)	133, 157, 321, 514, 520, 585, 612, 1193, 1399
Lsp1109I	GCAGC	5 cut(s)	646, 649, 763, 791, 1073
LweI	GCATC	3 cut(s)	145, 396, 1073
MaeI	CTAG	2 cut(s)	791, 1313
MaeII	ACGT	1 cut(s)	204
MaeIII	GTNAC	4 cut(s)	59, 400, 1150, 1229
MalI	GATC	5 cut(s)	216, 420, 539, 819, 870
MboI	GATC	5 cut(s)	214, 418, 537, 817, 868
MboII	GAAGA	9 cut(s)	47, 248, 347, 548, 776, 981, 1187, 1395, 1420
MfeI	CAATTG	1 cut(s)	759
MflI	RGATCY	2 cut(s)	418, 817
MhlI	GDGCHC	1 cut(s)	1333
MluCI	AATT	7 cut(s)	358, 468, 567, 748, 759, 1020, 1111
Mly113I	GGCGCC	1 cut(s)	787
MmeI	TCCRAC	1 cut(s)	415
Mph1103I	ATGCAT	1 cut(s)	1101
MseI	TTAA	4 cut(s)	546, 570, 909, 1236
MslI	CAYNNNNRTG	1 cut(s)	1069
MspCI	CTTAAG	1 cut(s)	908
MspR9I	CCNGG	1 cut(s)	600
MunI	CAATTG	1 cut(s)	759
MvaI	CCWGG	1 cut(s)	600
MwoI	GCNNNNNNNGC	4 cut(s)	98, 463, 510, 659
NarI	GGCGCC	1 cut(s)	787
NdeII	GATC	5 cut(s)	214, 418, 537, 817, 868
NlaIII	CATG	9 cut(s)	47, 294, 440, 659, 833, 968, 983, 1099, 1202
NlaIV	GGNNCC	5 cut(s)	118, 141, 142, 322, 788
NmuCI	GTSAC	1 cut(s)	59
NsiI	ATGCAT	1 cut(s)	1101
NspI	RCATGY	2 cut(s)	659, 1099
PaeI	GCATGC	1 cut(s)	1099
PagI	TCATGA	3 cut(s)	43, 436, 1198
PaqCI	CACCTGC	1 cut(s)	509
PcsI	WCGNNNNNNNCGW	1 cut(s)	84
PfeI	GAWTC	1 cut(s)	937
PflMI	CCANNNNNTGG	3 cut(s)	150, 291, 649
PfoI	TCCNGGA	1 cut(s)	598
PkrI	GCNGC	5 cut(s)	661, 664, 778, 781, 1063
PluTI	GGCGCC	1 cut(s)	790
PpuMI	RGGWCCY	1 cut(s)	140
PshBI	ATTAAT	1 cut(s)	570
PsiI	TTATAA	1 cut(s)	999
Psp5II	RGGWCCY	1 cut(s)	140
Psp6I	CCWGG	1 cut(s)	598
PspFI	CCCAGC	1 cut(s)	143
PspGI	CCWGG	1 cut(s)	598
PspN4I	GGNNCC	5 cut(s)	118, 141, 142, 322, 788
PspPI	GGNCC	1 cut(s)	140
PspPPI	RGGWCCY	1 cut(s)	140
PstI	CTGCAG	1 cut(s)	781
PsuI	RGATCY	2 cut(s)	418, 817
RsaI	GTAC	3 cut(s)	410, 431, 812
RsaNI	GTAC	3 cut(s)	409, 430, 811
RseI	CAYNNNNRTG	1 cut(s)	1069
SaqAI	TTAA	4 cut(s)	546, 570, 909, 1236
SatI	GCNGC	5 cut(s)	660, 663, 777, 780, 1062
Sau3AI	GATC	5 cut(s)	214, 418, 537, 817, 868
Sau96I	GGNCC	1 cut(s)	140
ScrFI	CCNGG	1 cut(s)	600
SduI	GDGCHC	1 cut(s)	1333
SfaNI	GCATC	3 cut(s)	145, 396, 1073
SfcI	CTRYAG	1 cut(s)	777
SfoI	GGCGCC	1 cut(s)	788
SinI	GGWCC	1 cut(s)	140
SmiMI	CAYNNNNRTG	1 cut(s)	1069
SmlI	CTYRAG	3 cut(s)	908, 1332, 1420
SmoI	CTYRAG	3 cut(s)	908, 1332, 1420
SphI	GCATGC	1 cut(s)	1099
Sse9I	AATT	7 cut(s)	358, 468, 567, 748, 759, 1020, 1111
SsiI	CCGC	1 cut(s)	99
SspDI	GGCGCC	1 cut(s)	786
SspI	AATATT	2 cut(s)	221, 303
SspMI	CTAG	2 cut(s)	791, 1313
StyD4I	CCNGG	1 cut(s)	598
StyI	CCWWGG	1 cut(s)	1088
TaaI	ACNGT	3 cut(s)	131, 408, 731
TaiI	ACGT	1 cut(s)	207
TaqI	TCGA	1 cut(s)	298
TasI	AATT	7 cut(s)	358, 468, 567, 748, 759, 1020, 1111
TfiI	GAWTC	1 cut(s)	937
Tru1I	TTAA	4 cut(s)	546, 570, 909, 1236
Tru9I	TTAA	4 cut(s)	546, 570, 909, 1236
TscAI	CASTG	3 cut(s)	64, 216, 411
TseFI	GTSAC	1 cut(s)	59
TseI	GCWGC	5 cut(s)	659, 662, 776, 779, 1061
Tsp45I	GTSAC	1 cut(s)	59
TspDTI	ATGAA	8 cut(s)	48, 60, 348, 352, 425, 1041, 1187, 1215
TspRI	CASTG	3 cut(s)	64, 216, 411
Van91I	CCANNNNNTGG	3 cut(s)	150, 291, 649
Vha464I	CTTAAG	1 cut(s)	908
VpaK11BI	GGWCC	1 cut(s)	140
VspI	ATTAAT	1 cut(s)	570
XagI	CCTNNNNNAGG	1 cut(s)	380
XceI	RCATGY	2 cut(s)	659, 1099
XmiI	GTMKAC	2 cut(s)	726, 921
XspI	CTAG	2 cut(s)	791, 1313
Zsp2I	ATGCAT	1 cut(s)	1101

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.