Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0012573

Physical Location & Seq

Genomic Coordinates Forward (+)

6690882 .. 6695677

Gene Structure 4796 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1038300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2091 bp

ATGAAGTATTTCACCCTAATTGTATCCGTACTTGTTATTCCACTACTCACCGTCCATGCAGCAAATAGCTGCAACCAAGCGTGCCCGGGTTACAGGCCCAGCCCGATTCAGTACCCTTTCGGGTTCTCATCGGGCTGCGAAATCCGGCTTAACTGCATTCCAAACGGCGGAATCGCAATCGGGGAGTTTCCAATCCAGTCCATAAACCATGACAGCATAAGAATCAACCTCAAGAGCCATTGCAACCGCCCCCTCCACACCCTCCGCAACTTCTACAGCCGGAAATACGCCCCGACGTCACGAAACGCGATTCTTCTTCAGAACTGTACGGAAGGGACGGCGTCGGGCAGCTGCGAGATTCCCAACCTTCTGGTGCAGGCGCGGTTTGACGCGCCGCCCAATTGCAGCTCCAACAGCAGCCTCAGCTGCTACGCCGAGACTAGAAAGGATGTCCCATTTTTGGACTTTGGAAGCGTCGAGAGGAAGGATTGCAAGTTCTTGCTGTCGTCGATTTCGGCGCCGCCGCAACTTTTGAATAGCTCGACGCCGGCGGCGCCGGTGATGCTGGAGGTGGAAGTGTTGGATTTGGGGTGGTGGCTTGAGGGTACTTGTGGGTGTGACAAGAACGCCAATTGTACGACCGTCGTATCGCCAAATGGGACTGAAGGATATAGATGCAACTGTAAGGAGGGGTTTCTTGGAGATGGATACGTTGCTGGGTCAGGCTGCTGGAAAGTCTCATCGACTTGCAACCCTGCGAAATACTTGTCTGGCCAATGTGGAGGAAAGTCCAGGTTCATCCTTTTGATTGGAGCCGTTTTTGTTGGAGCTTCATTGATGATCAGTGTGGCTCTGCTATGCTGCTTCATTCGGCGACGATCCAAGCTGAAAGCGAGACACAGCACGAAACGCCTTCTATCCGCAGCTACAGGCAACTGCAACATTCCCTTCTACTCCTACAAAGAAATCGAAAAGGCCACAAATAGCTTCTCCGACAAGCAAAGGCTGGGGAACGGAGCCTATGGAACTGTTTATGCTGGTAAACTCCATGACGAATGGGTTGCCATAAAGAGAATCAAACACAGAGGACACGAAAGCATGGACCAAGTCATGAACGAGATCAAGCTCATTTCATCTGTAAGGCACCCGAATTTAGTCCAGTTATTAGGTTGCTCCATTGAACACGGTGAGCAGATTCTTGTGTACGAGTTCATGGAAAATGGGACATTGTGTCAGCATTTGCAAAGAGAGAGAGGGGAAGGGCTTTCTTGGCTCTCACGCCTGAACATTGCCGCTGAAACTGCTCAAGCAATATCCCATCTTCACTCCGCTTCTAACCCTCCGATATATCACAGAGACATCAAGTCGAGCAACATTCTTTTGGACTGCAAGTTCAGATCCAAAGTAGCGGATTTTGGCCTTTCAAGGCTAGGGATGGTGGAAACCTCCCACATTTCAACGGCTCCGCAGGGGACTCCGGGTTATCTTGACCCTCAATACCATCAGAACTTCCATCTCTCCGATAAAAGCGATGTCTATAGCTTTGGTGTGGTGCTTGCTGAGATCATATCAGGGTTGAAAGCTGTAGATTTTAACCGCCCACCGGATGAAGTAAACTTGGCAGCTCTAGCAACTGACAGAATCGGAAGGGCGTGTTTAAATGAAATCATAGACCCCTTTCTCGAGCCCTGTATGGATGACGCTTGGACCCTTTCGTCGGTTCAAAAGGTCGCTGAATTGGCATTCAGATGCCTTGCATTTCACCGAGACATGAGACCGACAATGATAGAAGTGACAGCTGAGTTGGAGCTCATCAAGCTAAGCAGATGGCCGTCTTCAGAAGAAAACACTTACACAGCTTCATCGGAGGGATCCTCCCATTGCTCTCCATCTAGCGTGAGCGAAAAGCCTCTAAGTATTACCAAATCCCGATCACTTCGAAGCGTCATTGCGCTGCAGAATGGGGATGCCGGTAGCTTCAATTCGAGCGAAAAGTTAAGGGACAATTCACCGGTCACTTTTCAAGATACGTGGTTAAGCGAACGGAGCTCGCCTTCATCGAACAGTTTGCTGAGTAATGTAATTCAGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004672 GO:0004674 GO:0005575 GO:0005623 GO:0005886 GO:0006464 GO:0006468 GO:0006793 GO:0006796 GO:0006807 GO:0007154 GO:0007165 GO:0007166 GO:0008150 GO:0008152 GO:0009987 GO:0016020 GO:0016301 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0019538 GO:0023052 GO:0036211 GO:0043170 GO:0043412 GO:0044237 GO:0044238 GO:0044260 GO:0044267 GO:0044464 GO:0050789 GO:0050794 GO:0050896 GO:0051716 GO:0065007 GO:0071704 GO:0071944 GO:0140096 GO:1901564

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

GUB_WAK_bind Pkinase Pkinase_Tyr

Protein Analysis

697

Amino Acids

76.5

Weight (kDa)

8.03

Isoelectric Point (pI)

46.44

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	331 - 599	5e-41	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	333 - 527	4.4e-40	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0012573)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_2g03470 FvH4_2g40520 FvH4_2g40520
malus_domestica	MD00G1038300.v1.1 MD08G1038900.v1.1 MD16G1038500.v1.1
prunus_persica	Prupe.1G386400_v2.0.a1
pyrus_communis	pycom08g03060
rosa_chinensis	RchiOBHm_Chr6g0300211
rosa_laevigata	RLG00000011357
rosa_multiflora	Rmu_sc0001670.1_g000034
rosa_roxburghii	Rroxscaffold_7G00168010
rosa_rugosa	Rorug05G0186800 Rorug06G0294100
rosa_samantha	Rh6AG406300 Rh6BG414200 Rh6CG421400 Rh6DG407000
rosa_wichuraiana	Rw6G035550

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AasI	GACNNNNNNGTC	1 cut(s)	1714
AatII	GACGTC	1 cut(s)	299
AccB1I	GGYRCC	3 cut(s)	517, 553, 1143
AccII	CGCG	3 cut(s)	308, 382, 392
AclWI	GGATC	4 cut(s)	873, 1392, 1866, 1879
AcoI	YGGCCR	2 cut(s)	772, 1829
AcsI	RAATTY	1 cut(s)	1150
AcuI	CTGAAG	3 cut(s)	302, 684, 1821
AcyI	GRCGYC	5 cut(s)	296, 341, 518, 545, 554
AfaI	GTAC	6 cut(s)	30, 113, 328, 607, 637, 1205
AfiI	CCNNNNNNNGG	4 cut(s)	167, 460, 1603, 1717
AgeI	ACCGGT	1 cut(s)	2011
AgsI	TTSAA	8 cut(s)	535, 1181, 1425, 1458, 1579, 1724, 1981, 2024
AhdI	GACNNNNNGTC	2 cut(s)	1230, 1363
AjnI	CCWGG	1 cut(s)	791
AloI	GAACNNNNNNTCC	4 cut(s)	479, 511, 1376, 1408
Alw21I	GWGCWC	2 cut(s)	1812, 2051
Alw26I	GTCTC	6 cut(s)	431, 742, 889, 1350, 1761, 1768
AlwI	GGATC	4 cut(s)	873, 1392, 1866, 1879
Ama87I	CYCGRG	2 cut(s)	85, 1682
AoxI	GGCC	5 cut(s)	95, 772, 975, 1417, 1829
ApoI	RAATTY	1 cut(s)	1150
ArsI	GACNNNNNNTTYG	2 cut(s)	752, 784
AsiGI	ACCGGT	1 cut(s)	2011
Asp700I	GAANNNNTTC	1 cut(s)	8
AspLEI	GCGC	5 cut(s)	382, 394, 520, 556, 1954
AspS9I	GGNCC	3 cut(s)	96, 1102, 1707
AsuC2I	CCSGG	3 cut(s)	86, 87, 1479
AsuHPI	GGTGA	6 cut(s)	4, 40, 571, 1199, 1754, 2001
AsuII	TTCGAA	1 cut(s)	1939
AvaI	CYCGRG	2 cut(s)	85, 1682
AvaII	GGWCC	2 cut(s)	1102, 1707
BaeGI	GKGCMC	1 cut(s)	86
BaeI	ACNNNNGTAYC	2 cut(s)	630, 663
BalI	TGGCCA	1 cut(s)	774
BamHI	GGATCC	1 cut(s)	1871
BanI	GGYRCC	3 cut(s)	517, 553, 1143
BanII	GRGCYC	3 cut(s)	1689, 1812, 2051
BbsI	GAAGAC	1 cut(s)	1827
Bbv12I	GWGCWC	2 cut(s)	1812, 2051
BbvCI	CCTCAGC	1 cut(s)	422
BccI	CCATC	7 cut(s)	698, 1326, 1429, 1509, 1521, 1821, 1897
BceAI	ACGGC	5 cut(s)	181, 354, 800, 1476, 1816
BcgI	CGANNNNNNTGC	4 cut(s)	1043, 1077, 2050, 2084
BciT130I	CCWGG	1 cut(s)	793
BciVI	GTATCC	2 cut(s)	34, 701
BclI	TGATCA	1 cut(s)	840
BcnI	CCSGG	3 cut(s)	86, 87, 1479
BcoDI	GTCTC	6 cut(s)	431, 742, 889, 1350, 1761, 1768
BfaI	CTAG	4 cut(s)	441, 1430, 1628, 1893
BfmI	CTRYAG	5 cut(s)	274, 927, 1537, 1584, 1955
BfoI	RGCGCY	2 cut(s)	521, 557
BfuI	GTATCC	2 cut(s)	34, 701
BlpI	GCTNAGC	1 cut(s)	1820
Bme1390I	CCNGG	4 cut(s)	86, 87, 793, 1479
Bme18I	GGWCC	2 cut(s)	1102, 1707
BmeRI	GACNNNNNGTC	2 cut(s)	1230, 1363
BmeT110I	CYCGRG	2 cut(s)	85, 1682
BmgT120I	GGNCC	3 cut(s)	96, 1102, 1707
BmiI	GGNNCC	8 cut(s)	519, 555, 814, 1018, 1145, 1464, 1709, 1873
BmrFI	CCNGG	4 cut(s)	86, 87, 793, 1479
BmsI	GCATC	4 cut(s)	552, 665, 1739, 1957
BpiI	GAAGAC	1 cut(s)	1827
BplI	GAGNNNNNCTC	2 cut(s)	1859, 1891
BpmI	CTGGAG	1 cut(s)	587
Bpu10I	CCTNAGC	1 cut(s)	422
Bpu1102I	GCTNAGC	1 cut(s)	1820
Bpu14I	TTCGAA	1 cut(s)	1939
BpuEI	CTTGAG	3 cut(s)	215, 620, 1290
BpuMI	CCSGG	3 cut(s)	86, 87, 1479
BsaAI	YACGTR	1 cut(s)	2031
BsaHI	GRCGYC	5 cut(s)	296, 341, 518, 545, 554
BsaI	GGTCTC	1 cut(s)	1768
BsaJI	CCNNGG	1 cut(s)	85
BsaWI	WCCGGW	2 cut(s)	1603, 2011
Bsc4I	CCNNNNNNNGG	4 cut(s)	167, 460, 1603, 1717
Bse118I	RCCGGY	4 cut(s)	547, 556, 1970, 2011
Bse1I	ACTGG	2 cut(s)	196, 1159
Bse3DI	GCAATG	4 cut(s)	238, 1287, 1879, 1947
BseBI	CCWGG	1 cut(s)	793
BseDI	CCNNGG	1 cut(s)	85
BseGI	GGATG	6 cut(s)	454, 798, 1440, 1612, 1702, 1972
BseLI	CCNNNNNNNGG	4 cut(s)	167, 460, 1603, 1717
BseMI	GCAATG	4 cut(s)	238, 1287, 1879, 1947
BseMII	CTCAG	4 cut(s)	436, 1551, 1791, 2063
BseNI	ACTGG	2 cut(s)	196, 1159
BseSI	GKGCMC	1 cut(s)	86
BseYI	CCCAGC	3 cut(s)	98, 716, 1006
BsgI	GTGCAG	1 cut(s)	395
Bsh1236I	CGCG	3 cut(s)	308, 382, 392
Bsh1285I	CGRYCG	1 cut(s)	642
BshFI	GGCC	5 cut(s)	97, 774, 977, 1419, 1831
BshNI	GGYRCC	3 cut(s)	517, 553, 1143
BshTI	ACCGGT	1 cut(s)	2011
BsiEI	CGRYCG	1 cut(s)	642
BsiHKAI	GWGCWC	2 cut(s)	1812, 2051
BsiHKCI	CYCGRG	2 cut(s)	85, 1682
BsiSI	CCGG	9 cut(s)	86, 145, 280, 548, 557, 1478, 1604, 1971, 2012
BslFI	GGGAC	6 cut(s)	349, 437, 673, 1237, 1486, 2015
BslI	CCNNNNNNNGG	4 cut(s)	167, 460, 1603, 1717
BsmAI	GTCTC	6 cut(s)	431, 742, 889, 1350, 1761, 1768
BsmFI	GGGAC	6 cut(s)	349, 437, 673, 1237, 1486, 2015
BsmI	GAATGC	2 cut(s)	156, 1742
BsnI	GGCC	5 cut(s)	97, 774, 977, 1419, 1831
Bso31I	GGTCTC	1 cut(s)	1768
BsoBI	CYCGRG	2 cut(s)	85, 1682
Bsp119I	TTCGAA	1 cut(s)	1939
Bsp1286I	GDGCHC	4 cut(s)	86, 1689, 1812, 2051
Bsp143I	GATC	7 cut(s)	840, 878, 1119, 1397, 1563, 1871, 1931
Bsp1720I	GCTNAGC	1 cut(s)	1820
BspANI	GGCC	5 cut(s)	97, 774, 977, 1419, 1831
BspCNI	CTCAG	4 cut(s)	435, 1552, 1792, 2064
BspFNI	CGCG	3 cut(s)	308, 382, 392
BspHI	TCATGA	1 cut(s)	1110
BspLI	GGNNCC	8 cut(s)	519, 555, 814, 1018, 1145, 1464, 1709, 1873
BspMAI	CTGCAG	1 cut(s)	1959
BspPI	GGATC	4 cut(s)	873, 1392, 1866, 1879
BspT104I	TTCGAA	1 cut(s)	1939
BspT107I	GGYRCC	3 cut(s)	517, 553, 1143
BspTNI	GGTCTC	1 cut(s)	1768
BsrDI	GCAATG	4 cut(s)	238, 1287, 1879, 1947
BsrFI	RCCGGY	4 cut(s)	547, 556, 1970, 2011
BsrI	ACTGG	2 cut(s)	196, 1159
BssAI	RCCGGY	4 cut(s)	547, 556, 1970, 2011
BssECI	CCNNGG	1 cut(s)	85
BssMI	GATC	7 cut(s)	840, 878, 1119, 1397, 1563, 1871, 1931
BssNI	GRCGYC	5 cut(s)	296, 341, 518, 545, 554
Bst2UI	CCWGG	1 cut(s)	793
Bst4CI	ACNGT	7 cut(s)	52, 326, 643, 683, 1030, 1187, 2066
BstACI	GRCGYC	5 cut(s)	296, 341, 518, 545, 554
BstBAI	YACGTR	1 cut(s)	2031
BstBI	TTCGAA	1 cut(s)	1939
BstC8I	GCNNGC	5 cut(s)	82, 378, 549, 1557, 2051
BstDEI	CTNAG	6 cut(s)	422, 1560, 1800, 1820, 1913, 2072
BstF5I	GGATG	6 cut(s)	454, 798, 1440, 1612, 1702, 1972
BstFNI	CGCG	3 cut(s)	308, 382, 392
BstH2I	RGCGCY	2 cut(s)	521, 557
BstHHI	GCGC	5 cut(s)	382, 394, 520, 556, 1954
BstKTI	GATC	7 cut(s)	843, 881, 1122, 1400, 1566, 1874, 1934
BstMAI	GTCTC	6 cut(s)	431, 742, 889, 1350, 1761, 1768
BstMBI	GATC	7 cut(s)	840, 878, 1119, 1397, 1563, 1871, 1931
BstMCI	CGRYCG	1 cut(s)	642
BstNI	CCWGG	1 cut(s)	793
BstSCI	CCNGG	4 cut(s)	84, 85, 791, 1477
BstSFI	CTRYAG	5 cut(s)	274, 927, 1537, 1584, 1955
BstSLI	GKGCMC	1 cut(s)	86
BstUI	CGCG	3 cut(s)	308, 382, 392
BstV2I	GAAGAC	1 cut(s)	1827
BstX2I	RGATCY	2 cut(s)	1397, 1871
BstXI	CCANNNNNNTGG	1 cut(s)	370
BstYI	RGATCY	2 cut(s)	1397, 1871
BsuI	GTATCC	2 cut(s)	34, 701
BsuRI	GGCC	5 cut(s)	97, 774, 977, 1419, 1831
BtgZI	GCGATG	1 cut(s)	1545
BtsCI	GGATG	6 cut(s)	454, 798, 1440, 1612, 1702, 1972
BtsIMutI	CAGTG	1 cut(s)	850
Cac8I	GCNNGC	5 cut(s)	82, 378, 549, 1557, 2051
CciI	TCATGA	1 cut(s)	1110
CfoI	GCGC	5 cut(s)	382, 394, 520, 556, 1954
Cfr10I	RCCGGY	4 cut(s)	547, 556, 1970, 2011
Cfr13I	GGNCC	3 cut(s)	96, 1102, 1707
Cfr9I	CCCGGG	1 cut(s)	85
CseI	GACGC	6 cut(s)	330, 398, 463, 553, 1709, 1933
Csp6I	GTAC	6 cut(s)	29, 112, 327, 606, 636, 1204
CspAI	ACCGGT	1 cut(s)	2011
CspCI	CAANNNNNGTGG	2 cut(s)	2012, 2047
CviAII	CATG	7 cut(s)	56, 209, 1049, 1099, 1111, 1213, 1771
CviQI	GTAC	6 cut(s)	29, 112, 327, 606, 636, 1204
DdeI	CTNAG	6 cut(s)	422, 1560, 1800, 1820, 1913, 2072
DinI	GGCGCC	2 cut(s)	519, 555
DpnI	GATC	7 cut(s)	842, 880, 1121, 1399, 1565, 1873, 1933
DpnII	GATC	7 cut(s)	840, 878, 1119, 1397, 1563, 1871, 1931
DraI	TTTAAA	1 cut(s)	1659
DrdI	GACNNNNNNGTC	1 cut(s)	1714
DriI	GACNNNNNGTC	2 cut(s)	1230, 1363
DseDI	GACNNNNNNGTC	1 cut(s)	1714
EaeI	YGGCCR	2 cut(s)	772, 1829
Eam1105I	GACNNNNNGTC	2 cut(s)	1230, 1363
EciI	GGCGGA	1 cut(s)	183
Ecl136II	GAGCTC	2 cut(s)	1810, 2049
Eco24I	GRGCYC	3 cut(s)	1689, 1812, 2051
Eco31I	GGTCTC	1 cut(s)	1768
Eco47I	GGWCC	2 cut(s)	1102, 1707
Eco53kI	GAGCTC	2 cut(s)	1810, 2049
Eco57I	CTGAAG	3 cut(s)	302, 684, 1821
Eco88I	CYCGRG	2 cut(s)	85, 1682
EcoICRI	GAGCTC	2 cut(s)	1810, 2049
EcoRII	CCWGG	1 cut(s)	791
EcoT38I	GRGCYC	3 cut(s)	1689, 1812, 2051
EgeI	GGCGCC	2 cut(s)	519, 555
EheI	GGCGCC	2 cut(s)	519, 555
FaeI	CATG	7 cut(s)	59, 212, 1052, 1102, 1114, 1216, 1774
FaqI	GGGAC	6 cut(s)	349, 437, 673, 1237, 1486, 2015
FatI	CATG	7 cut(s)	55, 208, 1048, 1098, 1110, 1212, 1770
FbaI	TGATCA	1 cut(s)	840
FokI	GGATG	6 cut(s)	461, 785, 1447, 1619, 1709, 1979
FriOI	GRGCYC	3 cut(s)	1689, 1812, 2051
FspBI	CTAG	4 cut(s)	441, 1430, 1628, 1893
GlaI	GCGC	5 cut(s)	381, 393, 519, 555, 1953
GsaI	CCCAGC	3 cut(s)	102, 720, 1010
GsuI	CTGGAG	1 cut(s)	587
HaeII	RGCGCY	2 cut(s)	521, 557
HaeIII	GGCC	5 cut(s)	97, 774, 977, 1419, 1831
HapII	CCGG	9 cut(s)	86, 145, 280, 548, 557, 1478, 1604, 1971, 2012
HgaI	GACGC	6 cut(s)	330, 398, 463, 553, 1709, 1933
HhaI	GCGC	5 cut(s)	382, 394, 520, 556, 1954
Hin1I	GRCGYC	5 cut(s)	296, 341, 518, 545, 554
Hin1II	CATG	7 cut(s)	59, 212, 1052, 1102, 1114, 1216, 1774
Hin6I	GCGC	5 cut(s)	380, 392, 518, 554, 1952
HinP1I	GCGC	5 cut(s)	380, 392, 518, 554, 1952
HinfI	GANTC	9 cut(s)	106, 171, 222, 310, 358, 1074, 1195, 1474, 1641
HpaII	CCGG	9 cut(s)	86, 145, 280, 548, 557, 1478, 1604, 1971, 2012
HphI	GGTGA	6 cut(s)	4, 40, 571, 1199, 1754, 2001
Hpy166II	GTNNAC	3 cut(s)	1043, 1204, 1615
Hpy188III	TCNNGA	8 cut(s)	232, 300, 478, 1111, 1487, 1682, 1929, 2024
Hpy8I	GTNNAC	3 cut(s)	1043, 1204, 1615
Hpy99I	CGWCG	8 cut(s)	298, 346, 479, 511, 547, 647, 879, 1720
HpyAV	CCTTC	9 cut(s)	326, 377, 478, 659, 923, 958, 1253, 1641, 2064
HpyCH4III	ACNGT	7 cut(s)	52, 326, 643, 683, 1030, 1187, 2066
HpyCH4IV	ACGT	3 cut(s)	296, 711, 2030
HpyF3I	CTNAG	6 cut(s)	422, 1560, 1800, 1820, 1913, 2072
HpySE526I	ACGT	3 cut(s)	296, 711, 2030
Hsp92I	GRCGYC	5 cut(s)	296, 341, 518, 545, 554
Hsp92II	CATG	7 cut(s)	59, 212, 1052, 1102, 1114, 1216, 1774
HspAI	GCGC	5 cut(s)	380, 392, 518, 554, 1952
KasI	GGCGCC	2 cut(s)	517, 553
KroI	GCCGGC	1 cut(s)	547
KroNI	GCCGGC	1 cut(s)	549
Ksp22I	TGATCA	1 cut(s)	840
Kzo9I	GATC	7 cut(s)	840, 878, 1119, 1397, 1563, 1871, 1931
LmnI	GCTCC	8 cut(s)	413, 812, 827, 1016, 1178, 1468, 1807, 2046
LweI	GCATC	4 cut(s)	552, 665, 1739, 1957
MaeI	CTAG	4 cut(s)	441, 1430, 1628, 1893
MaeII	ACGT	3 cut(s)	296, 711, 2030
MaeIII	GTNAC	5 cut(s)	89, 297, 617, 1792, 2014
MalI	GATC	7 cut(s)	842, 880, 1121, 1399, 1565, 1873, 1933
MboI	GATC	7 cut(s)	840, 878, 1119, 1397, 1563, 1871, 1931
MboII	GAAGA	5 cut(s)	305, 308, 1313, 1827, 1853
MfeI	CAATTG	2 cut(s)	400, 631
MflI	RGATCY	2 cut(s)	1397, 1871
MhlI	GDGCHC	4 cut(s)	86, 1689, 1812, 2051
MlsI	TGGCCA	1 cut(s)	774
MluCI	AATT	8 cut(s)	18, 400, 631, 1150, 1736, 1981, 2005, 2082
MluNI	TGGCCA	1 cut(s)	774
Mly113I	GGCGCC	2 cut(s)	518, 554
MlyI	GAGTC	1 cut(s)	1468
MmeI	TCCRAC	5 cut(s)	435, 561, 805, 1017, 1785
Mox20I	TGGCCA	1 cut(s)	774
MreI	CGCCGGCG	1 cut(s)	547
MroNI	GCCGGC	1 cut(s)	547
MroXI	GAANNNNTTC	1 cut(s)	8
MscI	TGGCCA	1 cut(s)	774
MseI	TTAA	5 cut(s)	150, 1593, 1658, 1997, 2036
MslI	CAYNNNNRTG	1 cut(s)	1747
Msp20I	TGGCCA	1 cut(s)	774
MspA1I	CMGCKG	4 cut(s)	351, 426, 1295, 1799
MspI	CCGG	9 cut(s)	86, 145, 280, 548, 557, 1478, 1604, 1971, 2012
MspR9I	CCNGG	4 cut(s)	86, 87, 793, 1479
MunI	CAATTG	2 cut(s)	400, 631
Mva1269I	GAATGC	2 cut(s)	156, 1742
MvaI	CCWGG	1 cut(s)	793
MvnI	CGCG	3 cut(s)	308, 382, 392
NaeI	GCCGGC	1 cut(s)	549
NarI	GGCGCC	2 cut(s)	518, 554
NciI	CCSGG	3 cut(s)	86, 87, 1479
NdeII	GATC	7 cut(s)	840, 878, 1119, 1397, 1563, 1871, 1931
NgoMIV	GCCGGC	1 cut(s)	547
NlaIII	CATG	7 cut(s)	59, 212, 1052, 1102, 1114, 1216, 1774
NlaIV	GGNNCC	8 cut(s)	519, 555, 814, 1018, 1145, 1464, 1709, 1873
NmeAIII	GCCGAG	1 cut(s)	460
NmuCI	GTSAC	4 cut(s)	297, 617, 1792, 2014
NspV	TTCGAA	1 cut(s)	1939
PaeR7I	CTCGAG	1 cut(s)	1682
PagI	TCATGA	1 cut(s)	1110
PcsI	WCGNNNNNNNCGW	1 cut(s)	2057
PctI	GAATGC	2 cut(s)	156, 1742
PdiI	GCCGGC	1 cut(s)	549
PdmI	GAANNNNTTC	1 cut(s)	8
PfeI	GAWTC	8 cut(s)	106, 171, 222, 310, 358, 1074, 1195, 1641
PflFI	GACNNNGTC	2 cut(s)	340, 1106
PinAI	ACCGGT	1 cut(s)	2011
PleI	GAGTC	1 cut(s)	1468
PluTI	GGCGCC	2 cut(s)	521, 557
PpsI	GAGTC	1 cut(s)	1468
Ppu21I	YACGTR	1 cut(s)	2031
Psp124BI	GAGCTC	2 cut(s)	1812, 2051
Psp6I	CCWGG	1 cut(s)	791
PspFI	CCCAGC	3 cut(s)	98, 716, 1006
PspGI	CCWGG	1 cut(s)	791
PspN4I	GGNNCC	8 cut(s)	519, 555, 814, 1018, 1145, 1464, 1709, 1873
PspPI	GGNCC	3 cut(s)	96, 1102, 1707
PstI	CTGCAG	1 cut(s)	1959
PsuI	RGATCY	2 cut(s)	1397, 1871
PsyI	GACNNNGTC	2 cut(s)	340, 1106
PvuII	CAGCTG	3 cut(s)	351, 426, 1799
RsaI	GTAC	6 cut(s)	30, 113, 328, 607, 637, 1205
RsaNI	GTAC	6 cut(s)	29, 112, 327, 606, 636, 1204
RseI	CAYNNNNRTG	1 cut(s)	1747
SacI	GAGCTC	2 cut(s)	1812, 2051
SaqAI	TTAA	5 cut(s)	150, 1593, 1658, 1997, 2036
Sau3AI	GATC	7 cut(s)	840, 878, 1119, 1397, 1563, 1871, 1931
Sau96I	GGNCC	3 cut(s)	96, 1102, 1707
SchI	GAGTC	1 cut(s)	1468
ScrFI	CCNGG	4 cut(s)	86, 87, 793, 1479
SduI	GDGCHC	4 cut(s)	86, 1689, 1812, 2051
SfaNI	GCATC	4 cut(s)	552, 665, 1739, 1957
SfcI	CTRYAG	5 cut(s)	274, 927, 1537, 1584, 1955
SfoI	GGCGCC	2 cut(s)	519, 555
Sfr274I	CTCGAG	1 cut(s)	1682
SfuI	TTCGAA	1 cut(s)	1939
SgrAI	CRCCGGYG	2 cut(s)	547, 556
SinI	GGWCC	2 cut(s)	1102, 1707
SlaI	CTCGAG	1 cut(s)	1682
SmaI	CCCGGG	1 cut(s)	87
SmiMI	CAYNNNNRTG	1 cut(s)	1747
SmlI	CTYRAG	4 cut(s)	230, 599, 1305, 1682
SmoI	CTYRAG	4 cut(s)	230, 599, 1305, 1682
Sse9I	AATT	8 cut(s)	18, 400, 631, 1150, 1736, 1981, 2005, 2082
SspDI	GGCGCC	2 cut(s)	517, 553
SspMI	CTAG	4 cut(s)	441, 1430, 1628, 1893
SstI	GAGCTC	2 cut(s)	1812, 2051
StyD4I	CCNGG	4 cut(s)	84, 85, 791, 1477
TaaI	ACNGT	7 cut(s)	52, 326, 643, 683, 1030, 1187, 2066
TaiI	ACGT	3 cut(s)	299, 714, 2033
TaqII	GACCGA	1 cut(s)	1792
TasI	AATT	8 cut(s)	18, 400, 631, 1150, 1736, 1981, 2005, 2082
TauI	GCSGC	5 cut(s)	397, 523, 526, 554, 1295
TfiI	GAWTC	8 cut(s)	106, 171, 222, 310, 358, 1074, 1195, 1641
Tru1I	TTAA	5 cut(s)	150, 1593, 1658, 1997, 2036
Tru9I	TTAA	5 cut(s)	150, 1593, 1658, 1997, 2036
TscAI	CASTG	1 cut(s)	850
TseFI	GTSAC	4 cut(s)	297, 617, 1792, 2014
Tsp45I	GTSAC	4 cut(s)	297, 617, 1792, 2014
TspGWI	ACGGA	4 cut(s)	16, 344, 1029, 2059
TspMI	CCCGGG	1 cut(s)	85
TspRI	CASTG	1 cut(s)	850
Tth111I	GACNNNGTC	2 cut(s)	340, 1106
VpaK11BI	GGWCC	2 cut(s)	1102, 1707
XapI	RAATTY	1 cut(s)	1150
XhoI	CTCGAG	1 cut(s)	1682
XmaI	CCCGGG	1 cut(s)	85
XmnI	GAANNNNTTC	1 cut(s)	8
XspI	CTAG	4 cut(s)	441, 1430, 1628, 1893
ZraI	GACGTC	1 cut(s)	297

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.