MD00G1045100.v1.1

Patatin-like phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0017577
Physical Location & Seq
Forward (+)
8246811 .. 8250459
3649 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1045100.v1.1.491

Sequence Viewer

Length: 1209 bp
ATGATGATGAGGCAGGAACATTCTCTCCGTGATGAAATAAACACATCAAGCTATGGTGATAGGATCACTGTTCTAAGCATCGATGGTGGAGGAATTAGAGGCATTCTCCCTGCAACCGTATTAACTTTCCTTGAATCCAAACTACAACTGGATGGAGAAGAAGTCCGAATTGCAGATTACTTCGACGTGATTGCAGGAACTAGCACAGGAGGCCTTATAACAGCCATGATGACAGCACCTGATCAAAACAAGCGACCTCTTTTGAGGCCAAAGATATTGTTCCATTCTATCTCAAACATTGTCCCAAAATATTCCCTCAATCCAGGAATAATCATGAGAATGAATGCCTTAAGGGGACCAAAATATGATGGCAAGTATCTCAGAAAGTTACTTAGAAAGATACTTGGATATCGAAGACTGCATGAAACTGTGACACGAATTGTTGTACCAACATTTGACATCAAATTGCTACAACCCCATGTCTTCTCAACCTTTGAGGCAGAGATTGATACTTCTGAGGATGCTTTGCTATCAGATGTTTGCATCGGTACTTCCTCAGCTCCAACTTATCATCCAGCTCATTATTTCAAAACTAAGGACTCAGGAAGAAATGATAGGGAATTTCACCTTGTTGATGGTGGGGTGGCAGCAAACAATCCTGCATTGCTAGCAATGAAGCCAACAGGGACAGTGTTCCCGGGGAGTCCAGATGCTCTTGCAGCACCACAAAGTCTAGATTATCAGAAATACATCATGCTTTCACTAGGCAGTGGGACTTCAAAAATAGAGAAGAAGTACGATGCTAAAGTGGCCTCAAAGTGGGGGATTTTGGGCTGGCTTTACAGGGATGGTCATTCCCCTTTGGTTGATGCTTTTGCATTTGCTAGTGGAGACATGGTAGATCTCCATATGTCCATGATTTTCAGGTCCATTAAATGTGAGCAGAACTACCTTCGTATTCAGGATGACACATTAAGTGGGCACACACCATCGACAGACAAGGCAACAAAGAAGAACATGAAGGAGCTTGTGAGGATTGGTGAGAGGCTCTTGCATAAACCTGTTTCAAGGATGAATCTTGATACAGGTATTTTTGAACCAGCGCAACATCAAGGCACCAATGAGCAAGCCCTCACTAGATTTGCAAACTTACTATCAGAGGAGCTAAGACTGCGCAAGGAAAGATTACAACGGTGGTCCTCCAATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

403

Amino Acids

45.04

Weight (kDa)

9.16

Isoelectric Point (pI)

40.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 25 - 224 1.2e-26 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017577)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15800
malus_domestica MD00G1045100.v1.1
prunus_persica Prupe.7G135400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0105081
rosa_laevigata RLG00000017401
rosa_multiflora Rmu_co8510209.1_g000001 Rmu_sc0005160.1_g000009
rosa_roxburghii Rroxscaffold_2G00138440
rosa_rugosa Rorug02G0126200
rosa_samantha Rh2BG185000 Rh2DG183100
rosa_wichuraiana Rw2G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 218
Acc16I TGCGCA 1 cut(s) 1175
AccB1I GGYRCC 1 cut(s) 1115
AclWI GGATC 1 cut(s) 71
AcsI RAATTY 1 cut(s) 620
AfaI GTAC 3 cut(s) 447, 550, 797
AfiI CCNNNNNNNGG 1 cut(s) 819
AflII CTTAAG 1 cut(s) 349
AgsI TTSAA 5 cut(s) 134, 589, 780, 1068, 1097
AjiI CACGTC 1 cut(s) 187
AjnI CCWGG 1 cut(s) 322
AloI GAACNNNNNNTCC 2 cut(s) 9, 41
AluBI AGCT 5 cut(s) 51, 560, 578, 1027, 1165
AluI AGCT 5 cut(s) 51, 560, 578, 1027, 1165
Alw26I GTCTC 1 cut(s) 885
AlwI GGATC 1 cut(s) 71
AlwNI CAGNNNCTG 1 cut(s) 239
Ama87I CYCGRG 1 cut(s) 697
AoxI GGCC 3 cut(s) 211, 266, 810
ApeKI GCWGC 2 cut(s) 647, 719
ApoI RAATTY 1 cut(s) 620
AspLEI GCGC 2 cut(s) 1105, 1176
AspS9I GGNCC 3 cut(s) 356, 927, 1197
AsuC2I CCSGG 2 cut(s) 698, 699
AsuHPI GGTGA 3 cut(s) 68, 617, 1052
AsuNHI GCTAGC 1 cut(s) 667
AvaI CYCGRG 1 cut(s) 697
AvaII GGWCC 3 cut(s) 356, 927, 1197
BaeGI GKGCMC 1 cut(s) 984
BanI GGYRCC 1 cut(s) 1115
BbsI GAAGAC 2 cut(s) 421, 475
BbvCI CCTCAGC 1 cut(s) 556
BbvI GCAGC 2 cut(s) 659, 731
BccI CCATC 6 cut(s) 77, 146, 362, 629, 842, 997
BcgI CGANNNNNNTGC 2 cut(s) 173, 207
BciT130I CCWGG 1 cut(s) 324
BclI TGATCA 1 cut(s) 241
BcnI CCSGG 2 cut(s) 698, 699
BcoDI GTCTC 1 cut(s) 885
BfaI CTAG 6 cut(s) 201, 668, 734, 764, 885, 1137
BfrI CTTAAG 1 cut(s) 349
BglII AGATCT 1 cut(s) 901
BisI GCNGC 2 cut(s) 648, 720
BlsI GCNGC 2 cut(s) 649, 721
Bme1390I CCNGG 3 cut(s) 324, 698, 699
Bme18I GGWCC 3 cut(s) 356, 927, 1197
BmeT110I CYCGRG 1 cut(s) 697
BmgBI CACGTC 1 cut(s) 187
BmgT120I GGNCC 3 cut(s) 356, 927, 1197
BmiI GGNNCC 2 cut(s) 357, 1117
BmrFI CCNGG 3 cut(s) 324, 698, 699
BmsI GCATC 6 cut(s) 87, 511, 552, 700, 790, 859
BmtI GCTAGC 1 cut(s) 671
BpiI GAAGAC 2 cut(s) 421, 475
BplI GAGNNNNNCTC 2 cut(s) 90, 122
Bpu10I CCTNAGC 1 cut(s) 556
BpuMI CCSGG 2 cut(s) 698, 699
Bsa29I ATCGAT 1 cut(s) 81
BsaJI CCNNGG 2 cut(s) 697, 698
BsaXI ACNNNNNCTCC 6 cut(s) 9, 39, 147, 177, 882, 912
Bsc4I CCNNNNNNNGG 1 cut(s) 819
Bse1I ACTGG 1 cut(s) 153
Bse3DI GCAATG 2 cut(s) 662, 678
BseBI CCWGG 1 cut(s) 324
BseCI ATCGAT 1 cut(s) 81
BseDI CCNNGG 2 cut(s) 697, 698
BseGI GGATG 6 cut(s) 157, 526, 571, 853, 970, 1077
BseLI CCNNNNNNNGG 1 cut(s) 819
BseMI GCAATG 2 cut(s) 662, 678
BseMII CTCAG 4 cut(s) 394, 507, 570, 615
BseNI ACTGG 1 cut(s) 153
BseRI GAGGAG 1 cut(s) 1175
BseSI GKGCMC 1 cut(s) 984
BseXI GCAGC 2 cut(s) 659, 731
BshFI GGCC 3 cut(s) 213, 268, 812
BshNI GGYRCC 1 cut(s) 1115
BshVI ATCGAT 1 cut(s) 81
BsiHKCI CYCGRG 1 cut(s) 697
BsiSI CCGG 1 cut(s) 698
BslFI GGGAC 4 cut(s) 287, 369, 700, 787
BslI CCNNNNNNNGG 1 cut(s) 819
BsmAI GTCTC 1 cut(s) 885
BsmFI GGGAC 4 cut(s) 287, 369, 700, 787
BsmI GAATGC 2 cut(s) 102, 349
BsnI GGCC 3 cut(s) 213, 268, 812
BsoBI CYCGRG 1 cut(s) 697
Bsp1286I GDGCHC 1 cut(s) 984
Bsp143I GATC 3 cut(s) 63, 241, 901
BspANI GGCC 3 cut(s) 213, 268, 812
BspCNI CTCAG 4 cut(s) 393, 508, 569, 614
BspDI ATCGAT 1 cut(s) 81
BspHI TCATGA 1 cut(s) 333
BspLI GGNNCC 2 cut(s) 357, 1117
BspOI GCTAGC 1 cut(s) 671
BspPI GGATC 1 cut(s) 71
BspT107I GGYRCC 1 cut(s) 1115
BspTI CTTAAG 1 cut(s) 349
BsrDI GCAATG 2 cut(s) 662, 678
BsrI ACTGG 1 cut(s) 153
BssECI CCNNGG 2 cut(s) 697, 698
BssMI GATC 3 cut(s) 63, 241, 901
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 5 cut(s) 70, 118, 430, 691, 1194
BstAFI CTTAAG 1 cut(s) 349
BstC8I GCNNGC 3 cut(s) 669, 836, 1128
BstDEI CTNAG 8 cut(s) 74, 380, 392, 516, 556, 594, 601, 1166
BstF5I GGATG 6 cut(s) 157, 526, 571, 853, 970, 1077
BstHHI GCGC 2 cut(s) 1105, 1176
BstKTI GATC 3 cut(s) 66, 244, 904
BstMAI GTCTC 1 cut(s) 885
BstMBI GATC 3 cut(s) 63, 241, 901
BstMWI GCNNNNNNNGC 5 cut(s) 210, 668, 719, 809, 1171
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 3 cut(s) 322, 696, 697
BstSLI GKGCMC 1 cut(s) 984
BstV1I GCAGC 2 cut(s) 659, 731
BstV2I GAAGAC 2 cut(s) 421, 475
BstX2I RGATCY 1 cut(s) 901
BstYI RGATCY 1 cut(s) 901
Bsu15I ATCGAT 1 cut(s) 81
BsuRI GGCC 3 cut(s) 213, 268, 812
BsuTUI ATCGAT 1 cut(s) 81
BtrI CACGTC 1 cut(s) 187
BtsCI GGATG 6 cut(s) 157, 526, 571, 853, 970, 1077
BtsI GCAGTG 1 cut(s) 775
BtsIMutI CAGTG 3 cut(s) 66, 696, 775
Cac8I GCNNGC 3 cut(s) 669, 836, 1128
CaiI CAGNNNCTG 1 cut(s) 239
CciI TCATGA 1 cut(s) 333
CfoI GCGC 2 cut(s) 1105, 1176
Cfr13I GGNCC 3 cut(s) 356, 927, 1197
Cfr9I CCCGGG 1 cut(s) 697
ClaI ATCGAT 1 cut(s) 81
Csp6I GTAC 3 cut(s) 446, 549, 796
CviAII CATG 8 cut(s) 226, 334, 422, 479, 754, 895, 916, 1018
CviQI GTAC 3 cut(s) 446, 549, 796
DdeI CTNAG 8 cut(s) 74, 380, 392, 516, 556, 594, 601, 1166
DpnI GATC 3 cut(s) 65, 243, 903
DpnII GATC 3 cut(s) 63, 241, 901
Eco147I AGGCCT 1 cut(s) 213
Eco32I GATATC 1 cut(s) 410
Eco47I GGWCC 3 cut(s) 356, 927, 1197
Eco88I CYCGRG 1 cut(s) 697
EcoRII CCWGG 1 cut(s) 322
EcoRV GATATC 1 cut(s) 410
FaeI CATG 8 cut(s) 229, 337, 425, 482, 757, 898, 919, 1021
FaqI GGGAC 4 cut(s) 287, 369, 700, 787
FatI CATG 8 cut(s) 225, 333, 421, 478, 753, 894, 915, 1017
FauNDI CATATG 1 cut(s) 909
FbaI TGATCA 1 cut(s) 241
Fnu4HI GCNGC 2 cut(s) 648, 720
FokI GGATG 6 cut(s) 164, 533, 558, 860, 977, 1084
Fsp4HI GCNGC 2 cut(s) 648, 720
FspBI CTAG 6 cut(s) 201, 668, 734, 764, 885, 1137
FspI TGCGCA 1 cut(s) 1175
GlaI GCGC 2 cut(s) 1104, 1175
GluI GCNGC 2 cut(s) 648, 720
HaeIII GGCC 3 cut(s) 213, 268, 812
HapII CCGG 1 cut(s) 698
HhaI GCGC 2 cut(s) 1105, 1176
Hin1II CATG 8 cut(s) 229, 337, 425, 482, 757, 898, 919, 1021
Hin6I GCGC 2 cut(s) 1103, 1174
HinP1I GCGC 2 cut(s) 1103, 1174
HinfI GANTC 4 cut(s) 134, 599, 703, 1075
HpaII CCGG 1 cut(s) 698
HphI GGTGA 3 cut(s) 68, 617, 1052
Hpy188I TCNGA 6 cut(s) 167, 383, 517, 535, 744, 1159
Hpy188III TCNNGA 6 cut(s) 334, 603, 707, 734, 962, 1079
Hpy99I CGWCG 1 cut(s) 188
HpyAV CCTTC 2 cut(s) 962, 1015
HpyCH4III ACNGT 5 cut(s) 70, 118, 430, 691, 1194
HpyCH4IV ACGT 1 cut(s) 186
HpyF10VI GCNNNNNNNGC 5 cut(s) 210, 668, 719, 809, 1171
HpyF3I CTNAG 8 cut(s) 74, 380, 392, 516, 556, 594, 601, 1166
HpySE526I ACGT 1 cut(s) 186
Hsp92II CATG 8 cut(s) 229, 337, 425, 482, 757, 898, 919, 1021
HspAI GCGC 2 cut(s) 1103, 1174
Ksp22I TGATCA 1 cut(s) 241
Kzo9I GATC 3 cut(s) 63, 241, 901
LmnI GCTCC 3 cut(s) 565, 1024, 1162
Lsp1109I GCAGC 2 cut(s) 659, 731
LweI GCATC 6 cut(s) 87, 511, 552, 700, 790, 859
MaeI CTAG 6 cut(s) 201, 668, 734, 764, 885, 1137
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 2 cut(s) 387, 430
MalI GATC 3 cut(s) 65, 243, 903
MboI GATC 3 cut(s) 63, 241, 901
MboII GAAGA 6 cut(s) 170, 426, 475, 618, 802, 1024
MfeI CAATTG 1 cut(s) 1204
MflI RGATCY 1 cut(s) 901
MhlI GDGCHC 1 cut(s) 984
MluCI AATT 6 cut(s) 93, 168, 438, 464, 620, 1204
MlyI GAGTC 2 cut(s) 593, 712
MmeI TCCRAC 1 cut(s) 587
MseI TTAA 4 cut(s) 122, 350, 933, 974
MslI CAYNNNNRTG 1 cut(s) 338
MspCI CTTAAG 1 cut(s) 349
MspI CCGG 1 cut(s) 698
MspR9I CCNGG 3 cut(s) 324, 698, 699
MunI CAATTG 1 cut(s) 1204
Mva1269I GAATGC 2 cut(s) 102, 349
MvaI CCWGG 1 cut(s) 324
MwoI GCNNNNNNNGC 5 cut(s) 210, 668, 719, 809, 1171
NciI CCSGG 2 cut(s) 698, 699
NdeI CATATG 1 cut(s) 909
NdeII GATC 3 cut(s) 63, 241, 901
NheI GCTAGC 1 cut(s) 667
NlaIII CATG 8 cut(s) 229, 337, 425, 482, 757, 898, 919, 1021
NlaIV GGNNCC 2 cut(s) 357, 1117
NmuCI GTSAC 1 cut(s) 430
NsbI TGCGCA 1 cut(s) 1175
PagI TCATGA 1 cut(s) 333
PceI AGGCCT 1 cut(s) 213
PctI GAATGC 2 cut(s) 102, 349
PfeI GAWTC 2 cut(s) 134, 1075
PfoI TCCNGGA 1 cut(s) 322
PkrI GCNGC 2 cut(s) 649, 721
PleI GAGTC 2 cut(s) 593, 711
PpsI GAGTC 2 cut(s) 593, 711
PsiI TTATAA 1 cut(s) 218
Psp6I CCWGG 1 cut(s) 322
PspGI CCWGG 1 cut(s) 322
PspN4I GGNNCC 2 cut(s) 357, 1117
PspPI GGNCC 3 cut(s) 356, 927, 1197
PstNI CAGNNNCTG 1 cut(s) 239
PsuI RGATCY 1 cut(s) 901
RsaI GTAC 3 cut(s) 447, 550, 797
RsaNI GTAC 3 cut(s) 446, 549, 796
RseI CAYNNNNRTG 1 cut(s) 338
SaqAI TTAA 4 cut(s) 122, 350, 933, 974
SatI GCNGC 2 cut(s) 648, 720
Sau3AI GATC 3 cut(s) 63, 241, 901
Sau96I GGNCC 3 cut(s) 356, 927, 1197
SchI GAGTC 2 cut(s) 593, 712
ScrFI CCNGG 3 cut(s) 324, 698, 699
SduI GDGCHC 1 cut(s) 984
SfaNI GCATC 6 cut(s) 87, 511, 552, 700, 790, 859
SinI GGWCC 3 cut(s) 356, 927, 1197
SmaI CCCGGG 1 cut(s) 699
SmiMI CAYNNNNRTG 1 cut(s) 338
SmlI CTYRAG 1 cut(s) 349
SmoI CTYRAG 1 cut(s) 349
Sse9I AATT 6 cut(s) 93, 168, 438, 464, 620, 1204
SseBI AGGCCT 1 cut(s) 213
SspI AATATT 1 cut(s) 311
SspMI CTAG 6 cut(s) 201, 668, 734, 764, 885, 1137
StuI AGGCCT 1 cut(s) 213
StyD4I CCNGG 3 cut(s) 322, 696, 697
TaaI ACNGT 5 cut(s) 70, 118, 430, 691, 1194
TaiI ACGT 1 cut(s) 189
TaqI TCGA 4 cut(s) 81, 183, 412, 992
TasI AATT 6 cut(s) 93, 168, 438, 464, 620, 1204
TfiI GAWTC 2 cut(s) 134, 1075
Tru1I TTAA 4 cut(s) 122, 350, 933, 974
Tru9I TTAA 4 cut(s) 122, 350, 933, 974
TscAI CASTG 3 cut(s) 73, 696, 775
TseFI GTSAC 1 cut(s) 430
TseI GCWGC 2 cut(s) 647, 719
Tsp45I GTSAC 1 cut(s) 430
TspDTI ATGAA 6 cut(s) 48, 356, 438, 689, 1034, 1088
TspGWI ACGGA 1 cut(s) 17
TspMI CCCGGG 1 cut(s) 697
TspRI CASTG 3 cut(s) 73, 696, 775
Vha464I CTTAAG 1 cut(s) 349
VpaK11BI GGWCC 3 cut(s) 356, 927, 1197
XapI RAATTY 1 cut(s) 620
XbaI TCTAGA 1 cut(s) 733
XcmI CCANNNNNNNNNTGG 1 cut(s) 145
XmaI CCCGGG 1 cut(s) 697
XspI CTAG 6 cut(s) 201, 668, 734, 764, 885, 1137
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.