Rw2G013880

Patatin-like phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr2
OG0017577
Physical Location & Seq
Reverse (-)
15089441 .. 15094678
5238 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw2G013880.1

Sequence Viewer

Length: 1164 bp
ATGGGGGTCTATGGTGATAGAATTACTGTTCTAAGCATTGATGGGGGTGGAATTAGAGGCATTATTCCTGGAACCATTTTGAGTTTCCTTGAATCCAAATTACAAGAATTAGATGGAGAAGAAGCGCGAATTGCAGACTACTTTGATGTGATTGCAGGAACAAGCACAGGAGGGCTAATAGCAACCATGTTGACAGCACCTGATGAAAAAAAGAGACCTCTTTTTGAGGCAAGAGATATAGTCCCATTCTATCTGAAGCATTGCCCCAGAATATTTCCTCAATCCAGTCGGAGAATTATGAGAATGTGTGCCTTAATTGGACCAAAATATGATGGCAAGTACCTGAGAAAGATGCTTCGTCAGATACTTGGAGCTAGAAGACTGCACGATACTGTTACACGAATTGTTATACCCACTTTTGACATCAAGCTGCTGCAACCCCATGCCTTCTCAACCTTTGAGGCAGAGATCGATGCTTCTGAGGATGCATTGTTATCAGATGTTTGCATCGGTACTTCGTCGGCTCCAACTTATCTTCCAGCTCATCACTTCAAAACTAGAGACTCAGGAGGAAATGAGAGGGAATTCCATCTTGTTGATGGTGGTGTGGCAGCAAACAATCCTGCATTGCTGGCAATGAAGCCAACAGGGACAGTGTTCCCAGGAAGTCGAGATGCTCTGTCTGCACCACAAAGCCTAGACTACAGCAAATACCTAGTGCTCTCTCTAGGCACTGGAACTTCAAAGACAGCGAAGAAGTACGATGCAAAAATGGCATCAAAGTGGGGGATCATGGGCTGGCTCTACAAAGATGGCCACTGCCCTTTAGTTGATGCTTTCACTTTTGCTAGTGGAGACATGGTTGACCTTCACATGTCATTGATTTTCAGGTCCATTAGTTGCGAGCATAACTACCTTCGAATCCAAGATGACACTTTGAGCGGGGCTGTATCGTCGACGGACAAGGCGACGAAGAAGAACTTGAAGGATCTTGTGAAGACTGGTGAGGGTCTGCTGCAGAAACCTGTTTCCAGGATGAATCACGACACTGGGATTTTTGAGCCTGTGGGTAATGAAGGCACCAATGAGAACGCTCTCATTAGGTTTGCAAAATCATTATCAGACGAAAGAAGACTGCGGAAGGAAAGATTCCACCAAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

387

Amino Acids

42.72

Weight (kDa)

8.64

Isoelectric Point (pI)

38.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 11 - 212 9.2e-29 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017577)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15800
malus_domestica MD00G1045100.v1.1
prunus_persica Prupe.7G135400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0105081
rosa_laevigata RLG00000017401
rosa_multiflora Rmu_co8510209.1_g000001 Rmu_sc0005160.1_g000009
rosa_roxburghii Rroxscaffold_2G00138440
rosa_rugosa Rorug02G0126200
rosa_samantha Rh2BG185000 Rh2DG183100
rosa_wichuraiana Rw2G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1079
AccBSI CCGCTC 1 cut(s) 942
AccI GTMKAC 1 cut(s) 956
AccII CGCG 1 cut(s) 127
AciI CCGC 2 cut(s) 942, 1138
AclWI GGATC 2 cut(s) 797, 996
AcoI YGGCCR 1 cut(s) 814
AcsI RAATTY 1 cut(s) 584
AcuI CTGAAG 1 cut(s) 275
AfaI GTAC 3 cut(s) 341, 514, 761
AflIII ACRYGT 1 cut(s) 873
AgsI TTSAA 4 cut(s) 92, 553, 744, 985
AjnI CCWGG 3 cut(s) 67, 661, 1031
AluBI AGCT 3 cut(s) 374, 430, 542
AluI AGCT 3 cut(s) 374, 430, 542
Alw21I GWGCWC 1 cut(s) 723
Alw26I GTCTC 3 cut(s) 208, 555, 849
AlwI GGATC 2 cut(s) 797, 996
AlwNI CAGNNNCTG 1 cut(s) 200
AoxI GGCC 1 cut(s) 814
ApeKI GCWGC 4 cut(s) 430, 433, 611, 1015
ApoI RAATTY 1 cut(s) 584
AspLEI GCGC 1 cut(s) 127
AspS9I GGNCC 2 cut(s) 320, 891
AsuHPI GGTGA 2 cut(s) 26, 1016
AsuII TTCGAA 1 cut(s) 919
AvaII GGWCC 2 cut(s) 320, 891
BalI TGGCCA 1 cut(s) 816
BanI GGYRCC 1 cut(s) 1079
BbsI GAAGAC 3 cut(s) 385, 1004, 1138
Bbv12I GWGCWC 1 cut(s) 723
BbvI GCAGC 4 cut(s) 417, 420, 623, 1002
BccI CCATC 6 cut(s) 35, 107, 326, 593, 597, 806
BciT130I CCWGG 3 cut(s) 69, 663, 1033
BcoDI GTCTC 3 cut(s) 208, 555, 849
BfaI CTAG 7 cut(s) 375, 558, 698, 716, 728, 849, 1162
BfmI CTRYAG 2 cut(s) 703, 1016
BisI GCNGC 4 cut(s) 431, 434, 612, 1016
BlsI GCNGC 4 cut(s) 432, 435, 613, 1017
Bme1390I CCNGG 3 cut(s) 69, 663, 1033
Bme18I GGWCC 2 cut(s) 320, 891
BmgT120I GGNCC 2 cut(s) 320, 891
BmiI GGNNCC 3 cut(s) 73, 525, 1081
BmrFI CCNGG 3 cut(s) 69, 663, 1033
BmrI ACTGGG 1 cut(s) 1059
BmsI GCATC 8 cut(s) 342, 463, 475, 516, 664, 754, 785, 823
BmuI ACTGGG 1 cut(s) 1059
BpiI GAAGAC 3 cut(s) 385, 1004, 1138
Bpu14I TTCGAA 1 cut(s) 919
Bsa29I ATCGAT 1 cut(s) 471
BsaI GGTCTC 1 cut(s) 208
BsaJI CCNNGG 1 cut(s) 661
BsaXI ACNNNNNCTCC 2 cut(s) 846, 876
Bse1I ACTGG 4 cut(s) 285, 739, 1006, 1054
Bse3DI GCAATG 3 cut(s) 259, 626, 642
BseBI CCWGG 3 cut(s) 69, 663, 1033
BseCI ATCGAT 1 cut(s) 471
BseDI CCNNGG 1 cut(s) 661
BseGI GGATG 2 cut(s) 490, 1041
BseMI GCAATG 3 cut(s) 259, 626, 642
BseMII CTCAG 3 cut(s) 335, 471, 579
BseNI ACTGG 4 cut(s) 285, 739, 1006, 1054
BseXI GCAGC 4 cut(s) 417, 420, 623, 1002
BsgI GTGCAG 2 cut(s) 368, 669
Bsh1236I CGCG 1 cut(s) 127
BshFI GGCC 1 cut(s) 816
BshNI GGYRCC 1 cut(s) 1079
BshVI ATCGAT 1 cut(s) 471
BsiHKAI GWGCWC 1 cut(s) 723
BslFI GGGAC 2 cut(s) 227, 664
BsmAI GTCTC 3 cut(s) 208, 555, 849
BsmFI GGGAC 2 cut(s) 227, 664
BsnI GGCC 1 cut(s) 816
Bso31I GGTCTC 1 cut(s) 208
Bsp119I TTCGAA 1 cut(s) 919
Bsp1286I GDGCHC 1 cut(s) 723
Bsp143I GATC 3 cut(s) 468, 789, 988
BspACI CCGC 2 cut(s) 942, 1138
BspANI GGCC 1 cut(s) 816
BspCNI CTCAG 3 cut(s) 336, 472, 578
BspDI ATCGAT 1 cut(s) 471
BspFNI CGCG 1 cut(s) 127
BspLI GGNNCC 3 cut(s) 73, 525, 1081
BspMAI CTGCAG 1 cut(s) 1020
BspPI GGATC 2 cut(s) 797, 996
BspT104I TTCGAA 1 cut(s) 919
BspT107I GGYRCC 1 cut(s) 1079
BspTNI GGTCTC 1 cut(s) 208
BsrBI CCGCTC 1 cut(s) 942
BsrDI GCAATG 3 cut(s) 259, 626, 642
BsrI ACTGG 4 cut(s) 285, 739, 1006, 1054
BssECI CCNNGG 1 cut(s) 661
BssMI GATC 3 cut(s) 468, 789, 988
Bst2UI CCWGG 3 cut(s) 69, 663, 1033
Bst4CI ACNGT 3 cut(s) 28, 394, 655
BstBI TTCGAA 1 cut(s) 919
BstC8I GCNNGC 3 cut(s) 633, 800, 905
BstDEI CTNAG 4 cut(s) 32, 344, 480, 565
BstF5I GGATG 2 cut(s) 490, 1041
BstFNI CGCG 1 cut(s) 127
BstHHI GCGC 1 cut(s) 127
BstKTI GATC 3 cut(s) 471, 792, 991
BstMAI GTCTC 3 cut(s) 208, 555, 849
BstMBI GATC 3 cut(s) 468, 789, 988
BstMWI GCNNNNNNNGC 4 cut(s) 131, 632, 683, 773
BstNI CCWGG 3 cut(s) 69, 663, 1033
BstNSI RCATGY 1 cut(s) 877
BstSCI CCNGG 3 cut(s) 67, 661, 1031
BstSFI CTRYAG 2 cut(s) 703, 1016
BstUI CGCG 1 cut(s) 127
BstV1I GCAGC 4 cut(s) 417, 420, 623, 1002
BstV2I GAAGAC 3 cut(s) 385, 1004, 1138
BstX2I RGATCY 1 cut(s) 988
BstYI RGATCY 1 cut(s) 988
Bsu15I ATCGAT 1 cut(s) 471
BsuRI GGCC 1 cut(s) 816
BsuTUI ATCGAT 1 cut(s) 471
BtsCI GGATG 2 cut(s) 490, 1041
BtsI GCAGTG 1 cut(s) 817
BtsIMutI CAGTG 4 cut(s) 660, 732, 817, 1047
Cac8I GCNNGC 3 cut(s) 633, 800, 905
CaiI CAGNNNCTG 1 cut(s) 200
CfoI GCGC 1 cut(s) 127
Cfr13I GGNCC 2 cut(s) 320, 891
ClaI ATCGAT 1 cut(s) 471
Csp6I GTAC 3 cut(s) 340, 513, 760
CviAII CATG 5 cut(s) 187, 443, 793, 859, 874
CviQI GTAC 3 cut(s) 340, 513, 760
DdeI CTNAG 4 cut(s) 32, 344, 480, 565
DpnI GATC 3 cut(s) 470, 791, 990
DpnII GATC 3 cut(s) 468, 789, 988
EaeI YGGCCR 1 cut(s) 814
Eco31I GGTCTC 1 cut(s) 208
Eco47I GGWCC 2 cut(s) 320, 891
Eco57I CTGAAG 1 cut(s) 275
EcoRI GAATTC 1 cut(s) 584
EcoRII CCWGG 3 cut(s) 67, 661, 1031
EcoT22I ATGCAT 1 cut(s) 490
FaeI CATG 5 cut(s) 190, 446, 796, 862, 877
FalI AAGNNNNNCTT 2 cut(s) 965, 997
FaqI GGGAC 2 cut(s) 227, 664
FatI CATG 5 cut(s) 186, 442, 792, 858, 873
FauI CCCGC 1 cut(s) 935
FblI GTMKAC 1 cut(s) 956
Fnu4HI GCNGC 4 cut(s) 431, 434, 612, 1016
FokI GGATG 2 cut(s) 497, 1048
Fsp4HI GCNGC 4 cut(s) 431, 434, 612, 1016
FspBI CTAG 7 cut(s) 375, 558, 698, 716, 728, 849, 1162
GlaI GCGC 1 cut(s) 126
GluI GCNGC 4 cut(s) 431, 434, 612, 1016
HaeIII GGCC 1 cut(s) 816
HhaI GCGC 1 cut(s) 127
Hin1II CATG 5 cut(s) 190, 446, 796, 862, 877
Hin6I GCGC 1 cut(s) 125
HinP1I GCGC 1 cut(s) 125
HincII GTYRAC 3 cut(s) 192, 865, 957
HindII GTYRAC 3 cut(s) 192, 865, 957
HinfI GANTC 5 cut(s) 92, 563, 921, 1039, 1149
HphI GGTGA 2 cut(s) 26, 1016
Hpy166II GTNNAC 3 cut(s) 192, 865, 957
Hpy188I TCNGA 6 cut(s) 255, 291, 363, 481, 499, 1123
Hpy188III TCNNGA 3 cut(s) 567, 671, 1043
Hpy8I GTNNAC 3 cut(s) 192, 865, 957
Hpy99I CGWCG 4 cut(s) 523, 958, 961, 973
HpyAV CCTTC 6 cut(s) 457, 878, 926, 979, 1070, 1135
HpyCH4III ACNGT 3 cut(s) 28, 394, 655
HpyF10VI GCNNNNNNNGC 4 cut(s) 131, 632, 683, 773
HpyF3I CTNAG 4 cut(s) 32, 344, 480, 565
Hsp92II CATG 5 cut(s) 190, 446, 796, 862, 877
HspAI GCGC 1 cut(s) 125
Kzo9I GATC 3 cut(s) 468, 789, 988
LmnI GCTCC 2 cut(s) 371, 529
Lsp1109I GCAGC 4 cut(s) 417, 420, 623, 1002
LweI GCATC 8 cut(s) 342, 463, 475, 516, 664, 754, 785, 823
MaeI CTAG 7 cut(s) 375, 558, 698, 716, 728, 849, 1162
MaeIII GTNAC 1 cut(s) 394
MalI GATC 3 cut(s) 470, 791, 990
MbiI CCGCTC 1 cut(s) 942
MboI GATC 3 cut(s) 468, 789, 988
MboII GAAGA 8 cut(s) 131, 390, 527, 766, 985, 988, 1009, 1143
MflI RGATCY 1 cut(s) 988
MhlI GDGCHC 1 cut(s) 723
MlsI TGGCCA 1 cut(s) 816
MluCI AATT 9 cut(s) 21, 51, 98, 107, 129, 294, 315, 402, 584
MluNI TGGCCA 1 cut(s) 816
MlyI GAGTC 1 cut(s) 557
MmeI TCCRAC 2 cut(s) 269, 551
Mox20I TGGCCA 1 cut(s) 816
Mph1103I ATGCAT 1 cut(s) 490
MscI TGGCCA 1 cut(s) 816
MseI TTAA 1 cut(s) 314
MslI CAYNNNNRTG 1 cut(s) 781
Msp20I TGGCCA 1 cut(s) 816
MspR9I CCNGG 3 cut(s) 69, 663, 1033
MvaI CCWGG 3 cut(s) 69, 663, 1033
MvnI CGCG 1 cut(s) 127
MwoI GCNNNNNNNGC 4 cut(s) 131, 632, 683, 773
NdeII GATC 3 cut(s) 468, 789, 988
NlaIII CATG 5 cut(s) 190, 446, 796, 862, 877
NlaIV GGNNCC 3 cut(s) 73, 525, 1081
NsiI ATGCAT 1 cut(s) 490
NspI RCATGY 1 cut(s) 877
NspV TTCGAA 1 cut(s) 919
PciI ACATGT 1 cut(s) 873
PcsI WCGNNNNNNNCGW 1 cut(s) 965
PfeI GAWTC 4 cut(s) 92, 921, 1039, 1149
PfoI TCCNGGA 2 cut(s) 67, 1031
PkrI GCNGC 4 cut(s) 432, 435, 613, 1017
PleI GAGTC 1 cut(s) 557
PpsI GAGTC 1 cut(s) 557
PscI ACATGT 1 cut(s) 873
Psp6I CCWGG 3 cut(s) 67, 661, 1031
PspGI CCWGG 3 cut(s) 67, 661, 1031
PspN4I GGNNCC 3 cut(s) 73, 525, 1081
PspPI GGNCC 2 cut(s) 320, 891
PstI CTGCAG 1 cut(s) 1020
PstNI CAGNNNCTG 1 cut(s) 200
PsuI RGATCY 1 cut(s) 988
RsaI GTAC 3 cut(s) 341, 514, 761
RsaNI GTAC 3 cut(s) 340, 513, 760
RseI CAYNNNNRTG 1 cut(s) 781
SalI GTCGAC 1 cut(s) 955
SaqAI TTAA 1 cut(s) 314
SatI GCNGC 4 cut(s) 431, 434, 612, 1016
Sau3AI GATC 3 cut(s) 468, 789, 988
Sau96I GGNCC 2 cut(s) 320, 891
SchI GAGTC 1 cut(s) 557
ScrFI CCNGG 3 cut(s) 69, 663, 1033
SduI GDGCHC 1 cut(s) 723
SfaNI GCATC 8 cut(s) 342, 463, 475, 516, 664, 754, 785, 823
SfcI CTRYAG 2 cut(s) 703, 1016
SfuI TTCGAA 1 cut(s) 919
SgrDI CGTCGACG 1 cut(s) 955
SinI GGWCC 2 cut(s) 320, 891
SmiMI CAYNNNNRTG 1 cut(s) 781
Sse9I AATT 9 cut(s) 21, 51, 98, 107, 129, 294, 315, 402, 584
SsiI CCGC 2 cut(s) 942, 1138
SspI AATATT 1 cut(s) 273
SspMI CTAG 7 cut(s) 375, 558, 698, 716, 728, 849, 1162
StyD4I CCNGG 3 cut(s) 67, 661, 1031
TaaI ACNGT 3 cut(s) 28, 394, 655
TaqI TCGA 4 cut(s) 471, 670, 919, 956
TasI AATT 9 cut(s) 21, 51, 98, 107, 129, 294, 315, 402, 584
TfiI GAWTC 4 cut(s) 92, 921, 1039, 1149
Tru1I TTAA 1 cut(s) 314
Tru9I TTAA 1 cut(s) 314
TscAI CASTG 4 cut(s) 660, 739, 824, 1054
TseI GCWGC 4 cut(s) 430, 433, 611, 1015
TspDTI ATGAA 4 cut(s) 219, 653, 1052, 1089
TspGWI ACGGA 1 cut(s) 974
TspRI CASTG 4 cut(s) 660, 739, 824, 1054
VpaK11BI GGWCC 2 cut(s) 320, 891
XapI RAATTY 1 cut(s) 584
XceI RCATGY 1 cut(s) 877
XcmI CCANNNNNNNNNTGG 1 cut(s) 596
XmiI GTMKAC 1 cut(s) 956
XspI CTAG 7 cut(s) 375, 558, 698, 716, 728, 849, 1162
Zsp2I ATGCAT 1 cut(s) 490
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.