Rh2BG185000

Patatin-like phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2B
OG0017577
Physical Location & Seq
Reverse (-)
16461407 .. 16467487
6081 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2BG185000.1

Sequence Viewer

Length: 765 bp
ATGTTGACAGCACCTGATGAAAAAAAGAGACCTCTTTTTGAGGCAAGAGATATAGTCCCATTCTATCTGAAGCATTGCCCCAGAATATTTCCTCAATCCAGTCGGAGAATTATGAGAATGTGTGCCTTAATTGGACCAAAATATGATGGCAAGTACCTGAGAAAGACGCTTCGTCAGATACTTGGAGCTAGAAGACAGCACGATACCGTTACACGAATTGTTATACCCACTTTTGACATCAAGCTGCTTCAACCCCATGCCTTTTCAACCTTTGAGGCAGAGATCGATGCTTCTGAGGATGCATTGTTATTAGATGTTTGCATCGGTACTTCGTCGGCTCCAACTTATCTTCCAGCTCATCACTTCAAAACTAGAGACTCAGGAGGAAATGAGAGGGAATTCCATCTTGTCGATGGTGGTGTGGCAGCAAACAATCCTGCATTGCTGGCAATGAAGCCAACAGGGACAGTGTTCCCAGGAAGTCGAGATGCTCTGTCTGCACCACAAAGCCTAGACTACAGCAAATACCTAGTGCTCTGTCTAGGCACTGGAACTTCAAAGACAGCGAAGAAGTACGATGCAAAAATGGCATCAAAGTGGGGGATCATGGGCTGGCTCTACAAAGATGGCCACTGCCCTTTAGTTGATGCTTTTACTTTTGCTAGTGGAGACATGGTTGACCTTTACATGTCATTGATTTTCAGGTCCATTAGTTGCGAGCATAACTACCTTCGAATCCAAGTCTGTGGATTGGAATCATCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

254

Amino Acids

28.37

Weight (kDa)

8.9

Isoelectric Point (pI)

50.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 29 - 150 2.4e-12 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017577)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15800
malus_domestica MD00G1045100.v1.1
prunus_persica Prupe.7G135400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0105081
rosa_laevigata RLG00000017401
rosa_multiflora Rmu_co8510209.1_g000001 Rmu_sc0005160.1_g000009
rosa_roxburghii Rroxscaffold_2G00138440
rosa_rugosa Rorug02G0126200
rosa_samantha Rh2BG185000 Rh2DG183100
rosa_wichuraiana Rw2G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 611
AcoI YGGCCR 1 cut(s) 628
AcsI RAATTY 1 cut(s) 398
AcuI CTGAAG 1 cut(s) 89
AfaI GTAC 3 cut(s) 155, 328, 575
AflIII ACRYGT 1 cut(s) 687
AgsI TTSAA 4 cut(s) 251, 267, 367, 558
AhdI GACNNNNNGTC 1 cut(s) 171
AjnI CCWGG 1 cut(s) 475
AluBI AGCT 3 cut(s) 188, 244, 356
AluI AGCT 3 cut(s) 188, 244, 356
Alw21I GWGCWC 1 cut(s) 537
Alw26I GTCTC 3 cut(s) 22, 369, 663
AlwI GGATC 1 cut(s) 611
AlwNI CAGNNNCTG 1 cut(s) 14
AoxI GGCC 1 cut(s) 628
ApeKI GCWGC 2 cut(s) 244, 425
ApoI RAATTY 1 cut(s) 398
AspS9I GGNCC 2 cut(s) 134, 705
AsuII TTCGAA 1 cut(s) 733
AvaII GGWCC 2 cut(s) 134, 705
BalI TGGCCA 1 cut(s) 630
BbsI GAAGAC 1 cut(s) 199
Bbv12I GWGCWC 1 cut(s) 537
BbvI GCAGC 2 cut(s) 231, 437
BccI CCATC 4 cut(s) 140, 407, 411, 620
BciT130I CCWGG 1 cut(s) 477
BcoDI GTCTC 3 cut(s) 22, 369, 663
BfaI CTAG 6 cut(s) 189, 372, 512, 530, 542, 663
BfmI CTRYAG 1 cut(s) 517
BisI GCNGC 2 cut(s) 245, 426
BlsI GCNGC 2 cut(s) 246, 427
Bme1390I CCNGG 1 cut(s) 477
Bme18I GGWCC 2 cut(s) 134, 705
BmeRI GACNNNNNGTC 1 cut(s) 171
BmgT120I GGNCC 2 cut(s) 134, 705
BmiI GGNNCC 1 cut(s) 339
BmrFI CCNGG 1 cut(s) 477
BmsI GCATC 7 cut(s) 277, 289, 330, 478, 568, 599, 637
BpiI GAAGAC 1 cut(s) 199
Bpu14I TTCGAA 1 cut(s) 733
Bsa29I ATCGAT 1 cut(s) 285
BsaBI GATNNNNATC 1 cut(s) 754
BsaI GGTCTC 1 cut(s) 22
BsaJI CCNNGG 1 cut(s) 475
BsaXI ACNNNNNCTCC 2 cut(s) 660, 690
Bse1I ACTGG 2 cut(s) 99, 553
Bse3DI GCAATG 3 cut(s) 73, 440, 456
Bse8I GATNNNNATC 1 cut(s) 754
BseBI CCWGG 1 cut(s) 477
BseCI ATCGAT 1 cut(s) 285
BseDI CCNNGG 1 cut(s) 475
BseGI GGATG 1 cut(s) 304
BseJI GATNNNNATC 1 cut(s) 754
BseMI GCAATG 3 cut(s) 73, 440, 456
BseMII CTCAG 3 cut(s) 149, 285, 393
BseNI ACTGG 2 cut(s) 99, 553
BseXI GCAGC 2 cut(s) 231, 437
BsgI GTGCAG 1 cut(s) 483
BshFI GGCC 1 cut(s) 630
BshVI ATCGAT 1 cut(s) 285
BsiHKAI GWGCWC 1 cut(s) 537
BslFI GGGAC 2 cut(s) 41, 478
BsmAI GTCTC 3 cut(s) 22, 369, 663
BsmFI GGGAC 2 cut(s) 41, 478
BsnI GGCC 1 cut(s) 630
Bso31I GGTCTC 1 cut(s) 22
Bsp119I TTCGAA 1 cut(s) 733
Bsp1286I GDGCHC 1 cut(s) 537
Bsp143I GATC 2 cut(s) 282, 603
BspANI GGCC 1 cut(s) 630
BspCNI CTCAG 3 cut(s) 150, 286, 392
BspDI ATCGAT 1 cut(s) 285
BspLI GGNNCC 1 cut(s) 339
BspPI GGATC 1 cut(s) 611
BspT104I TTCGAA 1 cut(s) 733
BspTNI GGTCTC 1 cut(s) 22
BsrDI GCAATG 3 cut(s) 73, 440, 456
BsrI ACTGG 2 cut(s) 99, 553
BssECI CCNNGG 1 cut(s) 475
BssMI GATC 2 cut(s) 282, 603
Bst2UI CCWGG 1 cut(s) 477
Bst4CI ACNGT 2 cut(s) 208, 469
BstBI TTCGAA 1 cut(s) 733
BstC8I GCNNGC 3 cut(s) 447, 614, 719
BstDEI CTNAG 3 cut(s) 158, 294, 379
BstF5I GGATG 1 cut(s) 304
BstKTI GATC 2 cut(s) 285, 606
BstMAI GTCTC 3 cut(s) 22, 369, 663
BstMBI GATC 2 cut(s) 282, 603
BstMWI GCNNNNNNNGC 3 cut(s) 446, 497, 587
BstNI CCWGG 1 cut(s) 477
BstNSI RCATGY 1 cut(s) 691
BstSCI CCNGG 1 cut(s) 475
BstSFI CTRYAG 1 cut(s) 517
BstV1I GCAGC 2 cut(s) 231, 437
BstV2I GAAGAC 1 cut(s) 199
BstXI CCANNNNNNTGG 1 cut(s) 746
Bsu15I ATCGAT 1 cut(s) 285
BsuRI GGCC 1 cut(s) 630
BsuTUI ATCGAT 1 cut(s) 285
BtsCI GGATG 1 cut(s) 304
BtsI GCAGTG 1 cut(s) 631
BtsIMutI CAGTG 3 cut(s) 474, 546, 631
Cac8I GCNNGC 3 cut(s) 447, 614, 719
CaiI CAGNNNCTG 1 cut(s) 14
Cfr13I GGNCC 2 cut(s) 134, 705
ClaI ATCGAT 1 cut(s) 285
CseI GACGC 1 cut(s) 175
Csp6I GTAC 3 cut(s) 154, 327, 574
CviAII CATG 4 cut(s) 257, 607, 673, 688
CviJI RGCY 9 cut(s) 188, 244, 338, 356, 457, 510, 612, 616, 630
CviKI_1 RGCY 9 cut(s) 188, 244, 338, 356, 457, 510, 612, 616, 630
CviQI GTAC 3 cut(s) 154, 327, 574
DdeI CTNAG 3 cut(s) 158, 294, 379
DpnI GATC 2 cut(s) 284, 605
DpnII GATC 2 cut(s) 282, 603
DriI GACNNNNNGTC 1 cut(s) 171
EaeI YGGCCR 1 cut(s) 628
Eam1105I GACNNNNNGTC 1 cut(s) 171
Eco31I GGTCTC 1 cut(s) 22
Eco47I GGWCC 2 cut(s) 134, 705
Eco57I CTGAAG 1 cut(s) 89
EcoRI GAATTC 1 cut(s) 398
EcoRII CCWGG 1 cut(s) 475
EcoT22I ATGCAT 1 cut(s) 304
FaeI CATG 4 cut(s) 260, 610, 676, 691
FaiI YATR 9 cut(s) 53, 113, 144, 224, 258, 608, 674, 689, 723
FaqI GGGAC 2 cut(s) 41, 478
FatI CATG 4 cut(s) 256, 606, 672, 687
Fnu4HI GCNGC 2 cut(s) 245, 426
FokI GGATG 1 cut(s) 311
Fsp4HI GCNGC 2 cut(s) 245, 426
FspBI CTAG 6 cut(s) 189, 372, 512, 530, 542, 663
GluI GCNGC 2 cut(s) 245, 426
HaeIII GGCC 1 cut(s) 630
HgaI GACGC 1 cut(s) 175
Hin1II CATG 4 cut(s) 260, 610, 676, 691
HincII GTYRAC 2 cut(s) 6, 679
HindII GTYRAC 2 cut(s) 6, 679
HinfI GANTC 3 cut(s) 377, 735, 755
Hpy166II GTNNAC 2 cut(s) 6, 679
Hpy188I TCNGA 4 cut(s) 69, 105, 177, 295
Hpy188III TCNNGA 3 cut(s) 381, 485, 762
Hpy8I GTNNAC 2 cut(s) 6, 679
Hpy99I CGWCG 1 cut(s) 337
HpyAV CCTTC 1 cut(s) 740
HpyCH4III ACNGT 2 cut(s) 208, 469
HpyCH4V TGCA 5 cut(s) 302, 321, 440, 500, 581
HpyF10VI GCNNNNNNNGC 3 cut(s) 446, 497, 587
HpyF3I CTNAG 3 cut(s) 158, 294, 379
Hsp92II CATG 4 cut(s) 260, 610, 676, 691
Kzo9I GATC 2 cut(s) 282, 603
LmnI GCTCC 2 cut(s) 185, 343
Lsp1109I GCAGC 2 cut(s) 231, 437
LweI GCATC 7 cut(s) 277, 289, 330, 478, 568, 599, 637
MaeI CTAG 6 cut(s) 189, 372, 512, 530, 542, 663
MaeIII GTNAC 1 cut(s) 208
MalI GATC 2 cut(s) 284, 605
MboI GATC 2 cut(s) 282, 603
MboII GAAGA 3 cut(s) 204, 341, 580
MhlI GDGCHC 1 cut(s) 537
MlsI TGGCCA 1 cut(s) 630
MluCI AATT 4 cut(s) 108, 129, 216, 398
MluNI TGGCCA 1 cut(s) 630
MlyI GAGTC 1 cut(s) 371
MmeI TCCRAC 2 cut(s) 83, 365
MnlI CCTC 7 cut(s) 34, 42, 102, 268, 289, 377, 387
Mox20I TGGCCA 1 cut(s) 630
Mph1103I ATGCAT 1 cut(s) 304
MscI TGGCCA 1 cut(s) 630
MseI TTAA 1 cut(s) 128
MslI CAYNNNNRTG 1 cut(s) 595
Msp20I TGGCCA 1 cut(s) 630
MspR9I CCNGG 1 cut(s) 477
MvaI CCWGG 1 cut(s) 477
MwoI GCNNNNNNNGC 3 cut(s) 446, 497, 587
NdeII GATC 2 cut(s) 282, 603
NlaIII CATG 4 cut(s) 260, 610, 676, 691
NlaIV GGNNCC 1 cut(s) 339
NsiI ATGCAT 1 cut(s) 304
NspI RCATGY 1 cut(s) 691
NspV TTCGAA 1 cut(s) 733
PciI ACATGT 1 cut(s) 687
PfeI GAWTC 2 cut(s) 735, 755
PkrI GCNGC 2 cut(s) 246, 427
PleI GAGTC 1 cut(s) 371
PpsI GAGTC 1 cut(s) 371
PscI ACATGT 1 cut(s) 687
Psp6I CCWGG 1 cut(s) 475
PspGI CCWGG 1 cut(s) 475
PspN4I GGNNCC 1 cut(s) 339
PspPI GGNCC 2 cut(s) 134, 705
PstNI CAGNNNCTG 1 cut(s) 14
RsaI GTAC 3 cut(s) 155, 328, 575
RsaNI GTAC 3 cut(s) 154, 327, 574
RseI CAYNNNNRTG 1 cut(s) 595
SaqAI TTAA 1 cut(s) 128
SatI GCNGC 2 cut(s) 245, 426
Sau3AI GATC 2 cut(s) 282, 603
Sau96I GGNCC 2 cut(s) 134, 705
SchI GAGTC 1 cut(s) 371
ScrFI CCNGG 1 cut(s) 477
SduI GDGCHC 1 cut(s) 537
SfaNI GCATC 7 cut(s) 277, 289, 330, 478, 568, 599, 637
SfcI CTRYAG 1 cut(s) 517
SfuI TTCGAA 1 cut(s) 733
SinI GGWCC 2 cut(s) 134, 705
SmiMI CAYNNNNRTG 1 cut(s) 595
Sse9I AATT 4 cut(s) 108, 129, 216, 398
SspI AATATT 1 cut(s) 87
SspMI CTAG 6 cut(s) 189, 372, 512, 530, 542, 663
StyD4I CCNGG 1 cut(s) 475
TaaI ACNGT 2 cut(s) 208, 469
TaqI TCGA 4 cut(s) 285, 411, 484, 733
TasI AATT 4 cut(s) 108, 129, 216, 398
TfiI GAWTC 2 cut(s) 735, 755
Tru1I TTAA 1 cut(s) 128
Tru9I TTAA 1 cut(s) 128
TscAI CASTG 3 cut(s) 474, 553, 638
TseI GCWGC 2 cut(s) 244, 425
TspDTI ATGAA 2 cut(s) 33, 467
TspRI CASTG 3 cut(s) 474, 553, 638
VpaK11BI GGWCC 2 cut(s) 134, 705
XapI RAATTY 1 cut(s) 398
XceI RCATGY 1 cut(s) 691
XcmI CCANNNNNNNNNTGG 1 cut(s) 410
XspI CTAG 6 cut(s) 189, 372, 512, 530, 542, 663
Zsp2I ATGCAT 1 cut(s) 304
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.