Rroxscaffold_2G00138440

Patatin-like phospholipase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0017577
Physical Location & Seq
Forward (+)
76584906 .. 76589000
4095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00138440.1

Sequence Viewer

Length: 870 bp
ATGAGAATGTGTGCCTTAATTGGACCAAAATATGATGGCAAGTACCTGAGAAAGATGCTTCGTCAGATACTTGGAGCTAGAAGACTGCATGAAACTGTTACACGAATTGTTATACCCACTTTTGACATCAAGCTGCTTCAACCCCATGCCTTCTCAACCTTTGAGGCAGAGATCGATGCTTCTGAGGATGCATTGTTATCAGATGTTTGCATCGGTACTTCGTCGGCTCCAACTTATCTTCCAGCTCATCACTTCAAAACTAGAGACTCAGGAGGAAATGAGAGGGAATTCCATCTTGTTGATGGTGGCGTGGCAGCAAACAATCCTGCATTGCTGGCAATGAAGCCACCAGGGACAGTGTTCCAAGGAATTCCAGATGCTCTGGCTGCACCACAAAGCCTAGACTACAGCAAATACCTAGTGCTCTCTCTAGGCAGTGGAACTTCAAAGACAGAGAAGAAGTACGATGCAAAAATGGCATCAAAGTGGGGGATCATGGGCTGGCTCTACAAAGATGGCCACTGCCCTTTAGTTGATGCTTTTACTTTTGCTAGTGGAGACATGGTTGACCTTCACATGTCATTGATTTTCAGGTCCATTAGTTGCGAGCATAACTACCTTCGAATCCAAGATGACACTTTGAGCGGGGATGTATCGTCGACAGACAAGGCGACGAAGAAGAACTTGAAGGATCTTGTGAAGACTGGTGAGGATCTGCTGCAGAAACCTGTTTCCAGGATGAATCATGACACTGGGATTTTTGAGCCAGCTGTGGGAAATGAAGGCACCAATGAGAACGCTCTCATTAGGTTTGCAAAAGCACTATCAGACGAAAGAAGACTGCGGAAGGAAAGATTCCACCAAGCCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Patatin

Protein Analysis

289

Amino Acids

32.05

Weight (kDa)

7.72

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Patatin PF01734 8 - 113 6.5e-13 Patatin-like phospholipase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0017577)

Species Orthologous Gene IDs
fragaria_vesca FvH4_1g15800
malus_domestica MD00G1045100.v1.1
prunus_persica Prupe.7G135400_v2.0.a1
rosa_chinensis RchiOBHm_Chr2g0105081
rosa_laevigata RLG00000017401
rosa_multiflora Rmu_co8510209.1_g000001 Rmu_sc0005160.1_g000009
rosa_roxburghii Rroxscaffold_2G00138440
rosa_rugosa Rorug02G0126200
rosa_samantha Rh2BG185000 Rh2DG183100
rosa_wichuraiana Rw2G013880

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 785
AccBSI CCGCTC 1 cut(s) 645
AccI GTMKAC 1 cut(s) 659
AciI CCGC 2 cut(s) 645, 844
AclWI GGATC 3 cut(s) 500, 699, 720
AcoI YGGCCR 1 cut(s) 517
AcsI RAATTY 2 cut(s) 287, 369
AfaI GTAC 3 cut(s) 44, 217, 464
AfiI CCNNNNNNNGG 1 cut(s) 773
AflIII ACRYGT 1 cut(s) 576
AgsI TTSAA 4 cut(s) 140, 256, 447, 688
AjnI CCWGG 2 cut(s) 349, 734
AluBI AGCT 4 cut(s) 77, 133, 245, 770
AluI AGCT 4 cut(s) 77, 133, 245, 770
Alw21I GWGCWC 1 cut(s) 426
Alw26I GTCTC 2 cut(s) 258, 552
AlwI GGATC 3 cut(s) 500, 699, 720
AoxI GGCC 1 cut(s) 517
ApeKI GCWGC 4 cut(s) 133, 314, 386, 718
ApoI RAATTY 2 cut(s) 287, 369
AspS9I GGNCC 2 cut(s) 23, 594
AsuHPI GGTGA 1 cut(s) 719
AsuII TTCGAA 1 cut(s) 622
AvaII GGWCC 2 cut(s) 23, 594
BalI TGGCCA 1 cut(s) 519
BanI GGYRCC 1 cut(s) 785
BbsI GAAGAC 3 cut(s) 88, 707, 844
Bbv12I GWGCWC 1 cut(s) 426
BbvI GCAGC 4 cut(s) 120, 326, 373, 705
BccI CCATC 4 cut(s) 29, 296, 300, 509
BciT130I CCWGG 2 cut(s) 351, 736
BcoDI GTCTC 2 cut(s) 258, 552
BfaI CTAG 7 cut(s) 78, 261, 401, 419, 431, 552, 868
BfmI CTRYAG 2 cut(s) 406, 719
BisI GCNGC 4 cut(s) 134, 315, 387, 719
BlsI GCNGC 4 cut(s) 135, 316, 388, 720
Bme1390I CCNGG 2 cut(s) 351, 736
Bme18I GGWCC 2 cut(s) 23, 594
BmgT120I GGNCC 2 cut(s) 23, 594
BmiI GGNNCC 2 cut(s) 228, 787
BmrFI CCNGG 2 cut(s) 351, 736
BmrI ACTGGG 1 cut(s) 762
BmsI GCATC 8 cut(s) 45, 166, 178, 219, 367, 457, 488, 526
BmuI ACTGGG 1 cut(s) 762
BpiI GAAGAC 3 cut(s) 88, 707, 844
Bpu14I TTCGAA 1 cut(s) 622
Bsa29I ATCGAT 1 cut(s) 174
BsaJI CCNNGG 2 cut(s) 350, 364
BsaXI ACNNNNNCTCC 2 cut(s) 549, 579
Bsc4I CCNNNNNNNGG 1 cut(s) 773
Bse1I ACTGG 2 cut(s) 709, 757
Bse3DI GCAATG 2 cut(s) 329, 345
BseBI CCWGG 2 cut(s) 351, 736
BseCI ATCGAT 1 cut(s) 174
BseDI CCNNGG 2 cut(s) 350, 364
BseGI GGATG 3 cut(s) 193, 655, 744
BseLI CCNNNNNNNGG 1 cut(s) 773
BseMI GCAATG 2 cut(s) 329, 345
BseMII CTCAG 3 cut(s) 38, 174, 282
BseNI ACTGG 2 cut(s) 709, 757
BseXI GCAGC 4 cut(s) 120, 326, 373, 705
BsgI GTGCAG 1 cut(s) 372
BshFI GGCC 1 cut(s) 519
BshNI GGYRCC 1 cut(s) 785
BshVI ATCGAT 1 cut(s) 174
BsiHKAI GWGCWC 1 cut(s) 426
BslFI GGGAC 1 cut(s) 367
BslI CCNNNNNNNGG 1 cut(s) 773
BsmAI GTCTC 2 cut(s) 258, 552
BsmFI GGGAC 1 cut(s) 367
BsnI GGCC 1 cut(s) 519
Bsp119I TTCGAA 1 cut(s) 622
Bsp1286I GDGCHC 1 cut(s) 426
Bsp143I GATC 4 cut(s) 171, 492, 691, 712
BspACI CCGC 2 cut(s) 645, 844
BspANI GGCC 1 cut(s) 519
BspCNI CTCAG 3 cut(s) 39, 175, 281
BspDI ATCGAT 1 cut(s) 174
BspHI TCATGA 1 cut(s) 745
BspLI GGNNCC 2 cut(s) 228, 787
BspMAI CTGCAG 1 cut(s) 723
BspPI GGATC 3 cut(s) 500, 699, 720
BspT104I TTCGAA 1 cut(s) 622
BspT107I GGYRCC 1 cut(s) 785
BsrBI CCGCTC 1 cut(s) 645
BsrDI GCAATG 2 cut(s) 329, 345
BsrI ACTGG 2 cut(s) 709, 757
BssECI CCNNGG 2 cut(s) 350, 364
BssMI GATC 4 cut(s) 171, 492, 691, 712
BssT1I CCWWGG 1 cut(s) 364
Bst2UI CCWGG 2 cut(s) 351, 736
Bst4CI ACNGT 2 cut(s) 97, 358
BstBI TTCGAA 1 cut(s) 622
BstC8I GCNNGC 4 cut(s) 336, 503, 608, 768
BstDEI CTNAG 3 cut(s) 47, 183, 268
BstF5I GGATG 3 cut(s) 193, 655, 744
BstKTI GATC 4 cut(s) 174, 495, 694, 715
BstMAI GTCTC 2 cut(s) 258, 552
BstMBI GATC 4 cut(s) 171, 492, 691, 712
BstMWI GCNNNNNNNGC 3 cut(s) 335, 386, 476
BstNI CCWGG 2 cut(s) 351, 736
BstNSI RCATGY 1 cut(s) 580
BstSCI CCNGG 2 cut(s) 349, 734
BstSFI CTRYAG 2 cut(s) 406, 719
BstV1I GCAGC 4 cut(s) 120, 326, 373, 705
BstV2I GAAGAC 3 cut(s) 88, 707, 844
BstX2I RGATCY 2 cut(s) 691, 712
BstYI RGATCY 2 cut(s) 691, 712
Bsu15I ATCGAT 1 cut(s) 174
BsuRI GGCC 1 cut(s) 519
BsuTUI ATCGAT 1 cut(s) 174
BtsCI GGATG 3 cut(s) 193, 655, 744
BtsI GCAGTG 2 cut(s) 442, 520
BtsIMutI CAGTG 4 cut(s) 363, 442, 520, 750
Cac8I GCNNGC 4 cut(s) 336, 503, 608, 768
CciI TCATGA 1 cut(s) 745
Cfr13I GGNCC 2 cut(s) 23, 594
ClaI ATCGAT 1 cut(s) 174
Csp6I GTAC 3 cut(s) 43, 216, 463
CviAII CATG 6 cut(s) 89, 146, 496, 562, 577, 746
CviQI GTAC 3 cut(s) 43, 216, 463
DdeI CTNAG 3 cut(s) 47, 183, 268
DpnI GATC 4 cut(s) 173, 494, 693, 714
DpnII GATC 4 cut(s) 171, 492, 691, 712
EaeI YGGCCR 1 cut(s) 517
Eco130I CCWWGG 1 cut(s) 364
Eco47I GGWCC 2 cut(s) 23, 594
EcoRI GAATTC 2 cut(s) 287, 369
EcoRII CCWGG 2 cut(s) 349, 734
EcoT14I CCWWGG 1 cut(s) 364
EcoT22I ATGCAT 1 cut(s) 193
ErhI CCWWGG 1 cut(s) 364
FaeI CATG 6 cut(s) 92, 149, 499, 565, 580, 749
FaiI YATR 9 cut(s) 33, 90, 113, 147, 497, 563, 578, 612, 747
FalI AAGNNNNNCTT 2 cut(s) 668, 700
FaqI GGGAC 1 cut(s) 367
FatI CATG 6 cut(s) 88, 145, 495, 561, 576, 745
FauI CCCGC 1 cut(s) 638
FblI GTMKAC 1 cut(s) 659
Fnu4HI GCNGC 4 cut(s) 134, 315, 387, 719
FokI GGATG 3 cut(s) 200, 662, 751
Fsp4HI GCNGC 4 cut(s) 134, 315, 387, 719
FspBI CTAG 7 cut(s) 78, 261, 401, 419, 431, 552, 868
GluI GCNGC 4 cut(s) 134, 315, 387, 719
HaeIII GGCC 1 cut(s) 519
Hin1II CATG 6 cut(s) 92, 149, 499, 565, 580, 749
HincII GTYRAC 2 cut(s) 568, 660
HindII GTYRAC 2 cut(s) 568, 660
HinfI GANTC 4 cut(s) 266, 624, 742, 855
HphI GGTGA 1 cut(s) 719
Hpy166II GTNNAC 2 cut(s) 568, 660
Hpy188I TCNGA 4 cut(s) 66, 184, 202, 829
Hpy188III TCNNGA 3 cut(s) 270, 374, 746
Hpy8I GTNNAC 2 cut(s) 568, 660
Hpy99I CGWCG 3 cut(s) 226, 661, 676
HpyAV CCTTC 6 cut(s) 160, 581, 629, 682, 776, 841
HpyCH4III ACNGT 2 cut(s) 97, 358
HpyCH4V TGCA 8 cut(s) 88, 191, 210, 329, 389, 470, 721, 815
HpyF10VI GCNNNNNNNGC 3 cut(s) 335, 386, 476
HpyF3I CTNAG 3 cut(s) 47, 183, 268
Hsp92II CATG 6 cut(s) 92, 149, 499, 565, 580, 749
Kzo9I GATC 4 cut(s) 171, 492, 691, 712
LmnI GCTCC 2 cut(s) 74, 232
Lsp1109I GCAGC 4 cut(s) 120, 326, 373, 705
LweI GCATC 8 cut(s) 45, 166, 178, 219, 367, 457, 488, 526
MaeI CTAG 7 cut(s) 78, 261, 401, 419, 431, 552, 868
MaeIII GTNAC 1 cut(s) 97
MalI GATC 4 cut(s) 173, 494, 693, 714
MbiI CCGCTC 1 cut(s) 645
MboI GATC 4 cut(s) 171, 492, 691, 712
MboII GAAGA 7 cut(s) 93, 230, 469, 688, 691, 712, 849
MflI RGATCY 2 cut(s) 691, 712
MhlI GDGCHC 1 cut(s) 426
MlsI TGGCCA 1 cut(s) 519
MluCI AATT 4 cut(s) 18, 105, 287, 369
MluNI TGGCCA 1 cut(s) 519
MlyI GAGTC 1 cut(s) 260
MmeI TCCRAC 1 cut(s) 254
MnlI CCTC 5 cut(s) 157, 178, 266, 276, 703
Mox20I TGGCCA 1 cut(s) 519
Mph1103I ATGCAT 1 cut(s) 193
MscI TGGCCA 1 cut(s) 519
MseI TTAA 1 cut(s) 17
MslI CAYNNNNRTG 1 cut(s) 484
Msp20I TGGCCA 1 cut(s) 519
MspA1I CMGCKG 1 cut(s) 770
MspR9I CCNGG 2 cut(s) 351, 736
MvaI CCWGG 2 cut(s) 351, 736
MwoI GCNNNNNNNGC 3 cut(s) 335, 386, 476
NdeII GATC 4 cut(s) 171, 492, 691, 712
NlaIII CATG 6 cut(s) 92, 149, 499, 565, 580, 749
NlaIV GGNNCC 2 cut(s) 228, 787
NsiI ATGCAT 1 cut(s) 193
NspI RCATGY 1 cut(s) 580
NspV TTCGAA 1 cut(s) 622
PagI TCATGA 1 cut(s) 745
PciI ACATGT 1 cut(s) 576
PfeI GAWTC 3 cut(s) 624, 742, 855
PfoI TCCNGGA 1 cut(s) 734
PkrI GCNGC 4 cut(s) 135, 316, 388, 720
PleI GAGTC 1 cut(s) 260
PpsI GAGTC 1 cut(s) 260
PscI ACATGT 1 cut(s) 576
Psp6I CCWGG 2 cut(s) 349, 734
PspGI CCWGG 2 cut(s) 349, 734
PspN4I GGNNCC 2 cut(s) 228, 787
PspPI GGNCC 2 cut(s) 23, 594
PstI CTGCAG 1 cut(s) 723
PsuI RGATCY 2 cut(s) 691, 712
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 3 cut(s) 44, 217, 464
RsaNI GTAC 3 cut(s) 43, 216, 463
RseI CAYNNNNRTG 1 cut(s) 484
SalI GTCGAC 1 cut(s) 658
SaqAI TTAA 1 cut(s) 17
SatI GCNGC 4 cut(s) 134, 315, 387, 719
Sau3AI GATC 4 cut(s) 171, 492, 691, 712
Sau96I GGNCC 2 cut(s) 23, 594
SchI GAGTC 1 cut(s) 260
ScrFI CCNGG 2 cut(s) 351, 736
SduI GDGCHC 1 cut(s) 426
SfaNI GCATC 8 cut(s) 45, 166, 178, 219, 367, 457, 488, 526
SfcI CTRYAG 2 cut(s) 406, 719
SfuI TTCGAA 1 cut(s) 622
SinI GGWCC 2 cut(s) 23, 594
SmiMI CAYNNNNRTG 1 cut(s) 484
Sse9I AATT 4 cut(s) 18, 105, 287, 369
SsiI CCGC 2 cut(s) 645, 844
SspMI CTAG 7 cut(s) 78, 261, 401, 419, 431, 552, 868
StyD4I CCNGG 2 cut(s) 349, 734
StyI CCWWGG 1 cut(s) 364
TaaI ACNGT 2 cut(s) 97, 358
TaqI TCGA 3 cut(s) 174, 622, 659
TasI AATT 4 cut(s) 18, 105, 287, 369
TfiI GAWTC 3 cut(s) 624, 742, 855
Tru1I TTAA 1 cut(s) 17
Tru9I TTAA 1 cut(s) 17
TscAI CASTG 4 cut(s) 363, 442, 527, 757
TseI GCWGC 4 cut(s) 133, 314, 386, 718
TspDTI ATGAA 4 cut(s) 105, 356, 755, 795
TspRI CASTG 4 cut(s) 363, 442, 527, 757
VpaK11BI GGWCC 2 cut(s) 23, 594
XapI RAATTY 2 cut(s) 287, 369
XceI RCATGY 1 cut(s) 580
XcmI CCANNNNNNNNNTGG 1 cut(s) 299
XmiI GTMKAC 1 cut(s) 659
XspI CTAG 7 cut(s) 78, 261, 401, 419, 431, 552, 868
Zsp2I ATGCAT 1 cut(s) 193
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.