MD00G1045800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0016253
Physical Location & Seq
Reverse (-)
8481374 .. 8482456
1083 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1045800.v1.1.491

Sequence Viewer

Length: 1083 bp
ATGGAAATGGAAATCCCTGATGATTTCTGCTCCTATGTTAGTGCACCCGCTAGTCCTGGTAGATATTGCAGCTCTGCCAATAACAACAATGTTTGCTTCTTTAGTGTCCCAACTAGTCCTAGGAAAAGGACCATTTCATCAACTTCTGATGACCATCCAACACCACCCATGACACCATATCACGATGCCAATTCCGACCTCGACGACTTTGAATTTGATACCAGTCACAGTTTCAATCTTGGTTCGGTTGGTAATGTGACTAGCCAGAAGGTGAAAGACTTGCTCGAACACAAGCAGCACGAACAAATGCAAACCATGGCATTTGCTGACGACCTTTTCTGCGGCGGAAAAGTTATCCCGCTTGCACCTCCACTCAAACTTCCACCCCGTCTTGGTCAACAAAGATATACCAACCGAAGCCCAACCCCATCTACCCAAGGGTCACCGAGTTTTGGGATCAAAATGCCGTTTTCGCATAGGACGTTGTGGAATGATGATTTTGATCCATTCATGGTGGCACTGCAGCATGTCAGGAAGGAGGAAAAGAGAGGAAAAGCAAAGACACAGGCTCGTGACATGTCCGACCTGAATGACCAACAGAATAAACAAACTGGCCTGCCAACACCAATTTCCTCACCTTTTGTGAACTCCCCAAGTCTGCTACAAAGTCCAAAAAGGCTGGCTCTGCCCAAAGGACTGGAGTTTGCAAGACAAGTTAGACTAATACGAATGGATCATTCCGAGAGATGCAGTGACCCCAAATTGGAGACGAGGATGCCTAAGAATAACATTGCTCAAGAAAGTGGGCCTTGTACAAAGCAAACCAAGGGGCAGAAAATCGGAAGGCTTTTATTGAGTGTGTCATTTGGGAAGGCAGATGATGAAGACAAAAAGGGAAAAGATCAAACTTCCGAAATATTGAAAAAGACAACATTTCTAAGGAGACTAAGCTTCAAGTCAGGAAGATTAACCAGCTGCAAAGCCGAGAAGAGGATATCTAATCAACTCACCAAGATGCCGCTAGTACAATTCAGGCCGAGGCTCTTACTCTGTATGGGTTATAAGGCAAGGTATGCAAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

361

Amino Acids

40.54

Weight (kDa)

9.55

Isoelectric Point (pI)

52.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016253)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06740
malus_domestica MD00G1045800.v1.1 MD03G1164300.v1.1
prunus_persica Prupe.4G260600_v2.0.a1
pyrus_communis pycom12561g00260
rosa_chinensis RchiOBHm_Chr6g0258931
rosa_laevigata RLG00000014570
rosa_multiflora Rmu_sc0004347.1_g000009
rosa_roxburghii Rroxscaffold_7G00206180
rosa_rugosa Rorug05G0584500
rosa_samantha Rh6AG100900 Rh6BG092800 Rh6CG090100 Rh6DG084300
rosa_wichuraiana Rw6G008760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1062
AciI CCGC 5 cut(s) 48, 342, 345, 359, 1019
AclWI GGATC 3 cut(s) 464, 497, 741
AcsI RAATTY 1 cut(s) 212
AfaI GTAC 2 cut(s) 814, 1026
AfiI CCNNNNNNNGG 4 cut(s) 392, 452, 763, 990
AflIII ACRYGT 1 cut(s) 576
AgsI TTSAA 4 cut(s) 212, 235, 922, 955
AhlI ACTAGT 1 cut(s) 113
AjnI CCWGG 1 cut(s) 55
AjuI GAANNNNNNNTTGG 2 cut(s) 613, 645
AluBI AGCT 3 cut(s) 72, 951, 975
AluI AGCT 3 cut(s) 72, 951, 975
Alw21I GWGCWC 1 cut(s) 46
Alw26I GTCTC 2 cut(s) 761, 937
Alw44I GTGCAC 1 cut(s) 42
AlwI GGATC 3 cut(s) 464, 497, 741
AoxI GGCC 3 cut(s) 613, 806, 1034
ApaLI GTGCAC 1 cut(s) 42
ApeKI GCWGC 4 cut(s) 69, 295, 523, 975
ApoI RAATTY 1 cut(s) 212
ArsI GACNNNNNNTTYG 2 cut(s) 197, 229
AspA2I CCTAGG 1 cut(s) 119
AspS9I GGNCC 2 cut(s) 129, 806
AsuHPI GGTGA 4 cut(s) 283, 435, 627, 1000
AvaII GGWCC 1 cut(s) 129
AvrII CCTAGG 1 cut(s) 119
BaeGI GKGCMC 1 cut(s) 46
BauI CACGAG 1 cut(s) 570
BbsI GAAGAC 1 cut(s) 891
Bbv12I GWGCWC 1 cut(s) 46
BbvI GCAGC 4 cut(s) 81, 307, 535, 962
BccI CCATC 2 cut(s) 162, 436
BceAI ACGGC 1 cut(s) 451
BciT130I CCWGG 1 cut(s) 57
BcoDI GTCTC 2 cut(s) 761, 937
BcuI ACTAGT 1 cut(s) 113
BfaI CTAG 5 cut(s) 51, 114, 120, 261, 1022
BfmI CTRYAG 1 cut(s) 521
BisI GCNGC 6 cut(s) 70, 296, 343, 524, 976, 1019
BlnI CCTAGG 1 cut(s) 119
BlsI GCNGC 6 cut(s) 71, 297, 344, 525, 977, 1020
Bme1390I CCNGG 1 cut(s) 57
Bme18I GGWCC 1 cut(s) 129
BmgT120I GGNCC 2 cut(s) 129, 806
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 4 cut(s) 175, 737, 765, 1005
BpiI GAAGAC 1 cut(s) 891
BpmI CTGGAG 1 cut(s) 719
BpuEI CTTGAG 1 cut(s) 780
BsaBI GATNNNNATC 2 cut(s) 153, 501
BsaJI CCNNGG 5 cut(s) 119, 315, 436, 825, 1037
Bsc4I CCNNNNNNNGG 4 cut(s) 392, 452, 763, 990
Bse1I ACTGG 3 cut(s) 222, 616, 702
Bse3DI GCAATG 1 cut(s) 789
Bse8I GATNNNNATC 2 cut(s) 153, 501
BseBI CCWGG 1 cut(s) 57
BseDI CCNNGG 5 cut(s) 119, 315, 436, 825, 1037
BseGI GGATG 2 cut(s) 154, 780
BseJI GATNNNNATC 2 cut(s) 153, 501
BseLI CCNNNNNNNGG 4 cut(s) 392, 452, 763, 990
BseMI GCAATG 1 cut(s) 789
BseNI ACTGG 3 cut(s) 222, 616, 702
BseSI GKGCMC 1 cut(s) 46
BseXI GCAGC 4 cut(s) 81, 307, 535, 962
BshFI GGCC 3 cut(s) 615, 808, 1036
BsiHKAI GWGCWC 1 cut(s) 46
BslFI GGGAC 1 cut(s) 92
BslI CCNNNNNNNGG 4 cut(s) 392, 452, 763, 990
BsmAI GTCTC 2 cut(s) 761, 937
BsmBI CGTCTC 1 cut(s) 761
BsmFI GGGAC 1 cut(s) 92
BsnI GGCC 3 cut(s) 615, 808, 1036
Bsp1286I GDGCHC 1 cut(s) 46
Bsp1407I TGTACA 1 cut(s) 812
Bsp143I GATC 4 cut(s) 456, 502, 733, 901
Bsp19I CCATGG 1 cut(s) 315
BspACI CCGC 5 cut(s) 48, 342, 345, 359, 1019
BspANI GGCC 3 cut(s) 615, 808, 1036
BspMAI CTGCAG 1 cut(s) 525
BspPI GGATC 3 cut(s) 464, 497, 741
BsrDI GCAATG 1 cut(s) 789
BsrGI TGTACA 1 cut(s) 812
BsrI ACTGG 3 cut(s) 222, 616, 702
BssECI CCNNGG 5 cut(s) 119, 315, 436, 825, 1037
BssMI GATC 4 cut(s) 456, 502, 733, 901
BssSI CACGAG 1 cut(s) 570
BssT1I CCWWGG 4 cut(s) 119, 315, 436, 825
Bst2BI CACGAG 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 57
Bst4CI ACNGT 1 cut(s) 230
Bst6I CTCTTC 1 cut(s) 983
BstAPI GCANNNNNTGC 1 cut(s) 1073
BstAUI TGTACA 1 cut(s) 812
BstC8I GCNNGC 3 cut(s) 363, 617, 681
BstDEI CTNAG 3 cut(s) 780, 938, 947
BstDSI CCRYGG 1 cut(s) 315
BstEII GGTNACC 1 cut(s) 441
BstF5I GGATG 2 cut(s) 154, 780
BstKTI GATC 4 cut(s) 459, 505, 736, 904
BstMAI GTCTC 2 cut(s) 761, 937
BstMBI GATC 4 cut(s) 456, 502, 733, 901
BstMWI GCNNNNNNNGC 3 cut(s) 472, 685, 1073
BstNI CCWGG 1 cut(s) 57
BstNSI RCATGY 2 cut(s) 530, 580
BstPI GGTNACC 1 cut(s) 441
BstSCI CCNGG 1 cut(s) 55
BstSFI CTRYAG 1 cut(s) 521
BstSLI GKGCMC 1 cut(s) 46
BstV1I GCAGC 4 cut(s) 81, 307, 535, 962
BstV2I GAAGAC 1 cut(s) 891
BstXI CCANNNNNNTGG 1 cut(s) 697
BsuRI GGCC 3 cut(s) 615, 808, 1036
BtgI CCRYGG 1 cut(s) 315
BtsCI GGATG 2 cut(s) 154, 780
BtsI GCAGTG 2 cut(s) 518, 757
BtsIMutI CAGTG 2 cut(s) 518, 757
Cac8I GCNNGC 3 cut(s) 363, 617, 681
Cfr13I GGNCC 2 cut(s) 129, 806
Csp6I GTAC 2 cut(s) 813, 1025
CviAII CATG 5 cut(s) 169, 316, 511, 527, 577
CviQI GTAC 2 cut(s) 813, 1025
DdeI CTNAG 3 cut(s) 780, 938, 947
DpnI GATC 4 cut(s) 458, 504, 735, 903
DpnII GATC 4 cut(s) 456, 502, 733, 901
Eam1104I CTCTTC 1 cut(s) 983
EarI CTCTTC 1 cut(s) 983
EciI GGCGGA 1 cut(s) 360
Eco130I CCWWGG 4 cut(s) 119, 315, 436, 825
Eco32I GATATC 1 cut(s) 996
Eco47I GGWCC 1 cut(s) 129
Eco91I GGTNACC 1 cut(s) 441
EcoO65I GGTNACC 1 cut(s) 441
EcoRII CCWGG 1 cut(s) 55
EcoRV GATATC 1 cut(s) 996
EcoT14I CCWWGG 4 cut(s) 119, 315, 436, 825
ErhI CCWWGG 4 cut(s) 119, 315, 436, 825
Esp3I CGTCTC 1 cut(s) 761
FaeI CATG 5 cut(s) 172, 319, 514, 530, 580
FalI AAGNNNNNCTT 2 cut(s) 793, 825
FaqI GGGAC 1 cut(s) 92
FatI CATG 5 cut(s) 168, 315, 510, 526, 576
FauI CCCGC 2 cut(s) 55, 366
Fnu4HI GCNGC 6 cut(s) 70, 296, 343, 524, 976, 1019
FokI GGATG 2 cut(s) 141, 787
Fsp4HI GCNGC 6 cut(s) 70, 296, 343, 524, 976, 1019
FspBI CTAG 5 cut(s) 51, 114, 120, 261, 1022
GluI GCNGC 6 cut(s) 70, 296, 343, 524, 976, 1019
GsuI CTGGAG 1 cut(s) 719
HaeIII GGCC 3 cut(s) 615, 808, 1036
Hin1II CATG 5 cut(s) 172, 319, 514, 530, 580
HincII GTYRAC 1 cut(s) 398
HindII GTYRAC 1 cut(s) 398
HindIII AAGCTT 1 cut(s) 949
HphI GGTGA 4 cut(s) 283, 435, 627, 1000
Hpy166II GTNNAC 3 cut(s) 44, 398, 646
Hpy188I TCNGA 6 cut(s) 148, 196, 583, 742, 842, 913
Hpy188III TCNNGA 5 cut(s) 182, 532, 572, 797, 960
Hpy8I GTNNAC 3 cut(s) 44, 398, 646
Hpy99I CGWCG 1 cut(s) 206
HpyAV CCTTC 4 cut(s) 262, 529, 837, 865
HpyCH4III ACNGT 1 cut(s) 230
HpyCH4IV ACGT 1 cut(s) 482
HpyCH4V TGCA 9 cut(s) 44, 69, 310, 365, 523, 707, 750, 978, 1076
HpyF10VI GCNNNNNNNGC 3 cut(s) 472, 685, 1073
HpyF3I CTNAG 3 cut(s) 780, 938, 947
HpySE526I ACGT 1 cut(s) 482
Hsp92II CATG 5 cut(s) 172, 319, 514, 530, 580
Kzo9I GATC 4 cut(s) 456, 502, 733, 901
LmnI GCTCC 1 cut(s) 35
Lsp1109I GCAGC 4 cut(s) 81, 307, 535, 962
LweI GCATC 4 cut(s) 175, 737, 765, 1005
MaeI CTAG 5 cut(s) 51, 114, 120, 261, 1022
MaeII ACGT 1 cut(s) 482
MaeIII GTNAC 5 cut(s) 224, 256, 441, 572, 752
MalI GATC 4 cut(s) 458, 504, 735, 903
MboI GATC 4 cut(s) 456, 502, 733, 901
MboII GAAGA 3 cut(s) 896, 975, 1000
MhlI GDGCHC 1 cut(s) 46
MluCI AATT 5 cut(s) 190, 212, 627, 761, 1028
MmeI TCCRAC 3 cut(s) 182, 219, 606
MnlI CCTC 8 cut(s) 209, 378, 532, 542, 643, 765, 984, 1032
MseI TTAA 1 cut(s) 968
MslI CAYNNNNRTG 1 cut(s) 1013
MspA1I CMGCKG 1 cut(s) 975
MspR9I CCNGG 1 cut(s) 57
MvaI CCWGG 1 cut(s) 57
MwoI GCNNNNNNNGC 3 cut(s) 472, 685, 1073
NcoI CCATGG 1 cut(s) 315
NdeII GATC 4 cut(s) 456, 502, 733, 901
NlaIII CATG 5 cut(s) 172, 319, 514, 530, 580
NmeAIII GCCGAG 2 cut(s) 1009, 1062
NmuCI GTSAC 5 cut(s) 224, 256, 441, 572, 752
NspI RCATGY 2 cut(s) 530, 580
PciI ACATGT 1 cut(s) 576
PcsI WCGNNNNNNNCGW 1 cut(s) 479
PkrI GCNGC 6 cut(s) 71, 297, 344, 525, 977, 1020
PscI ACATGT 1 cut(s) 576
PsiI TTATAA 1 cut(s) 1062
Psp6I CCWGG 1 cut(s) 55
PspEI GGTNACC 1 cut(s) 441
PspGI CCWGG 1 cut(s) 55
PspPI GGNCC 2 cut(s) 129, 806
PstI CTGCAG 1 cut(s) 525
PvuII CAGCTG 1 cut(s) 975
RsaI GTAC 2 cut(s) 814, 1026
RsaNI GTAC 2 cut(s) 813, 1025
RseI CAYNNNNRTG 1 cut(s) 1013
SaqAI TTAA 1 cut(s) 968
SatI GCNGC 6 cut(s) 70, 296, 343, 524, 976, 1019
Sau3AI GATC 4 cut(s) 456, 502, 733, 901
Sau96I GGNCC 2 cut(s) 129, 806
ScrFI CCNGG 1 cut(s) 57
SduI GDGCHC 1 cut(s) 46
SfaNI GCATC 4 cut(s) 175, 737, 765, 1005
SfcI CTRYAG 1 cut(s) 521
SinI GGWCC 1 cut(s) 129
SmiMI CAYNNNNRTG 1 cut(s) 1013
SmlI CTYRAG 1 cut(s) 795
SmoI CTYRAG 1 cut(s) 795
SpeI ACTAGT 1 cut(s) 113
Sse9I AATT 5 cut(s) 190, 212, 627, 761, 1028
SsiI CCGC 5 cut(s) 48, 342, 345, 359, 1019
SspI AATATT 1 cut(s) 918
SspMI CTAG 5 cut(s) 51, 114, 120, 261, 1022
StyD4I CCNGG 1 cut(s) 55
StyI CCWWGG 4 cut(s) 119, 315, 436, 825
TaaI ACNGT 1 cut(s) 230
TaiI ACGT 1 cut(s) 485
TaqI TCGA 2 cut(s) 201, 285
TasI AATT 5 cut(s) 190, 212, 627, 761, 1028
TatI WGTACW 2 cut(s) 812, 1024
TauI GCSGC 2 cut(s) 345, 1021
Tru1I TTAA 1 cut(s) 968
Tru9I TTAA 1 cut(s) 968
TscAI CASTG 2 cut(s) 525, 757
TseFI GTSAC 5 cut(s) 224, 256, 441, 572, 752
TseI GCWGC 4 cut(s) 69, 295, 523, 975
Tsp45I GTSAC 5 cut(s) 224, 256, 441, 572, 752
TspDTI ATGAA 3 cut(s) 126, 499, 897
TspRI CASTG 2 cut(s) 525, 757
VneI GTGCAC 1 cut(s) 42
VpaK11BI GGWCC 1 cut(s) 129
XapI RAATTY 1 cut(s) 212
XceI RCATGY 2 cut(s) 530, 580
XmaJI CCTAGG 1 cut(s) 119
XspI CTAG 5 cut(s) 51, 114, 120, 261, 1022
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.