MD03G1164300.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0016253
Physical Location & Seq
Forward (+)
21689102 .. 21690181
1080 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1164300.v1.1.491

Sequence Viewer

Length: 1080 bp
ATGGAAATCCCAGATGATTTCTGCTCCTATGTCAGTGCACCTGCTACCCCTGGAAGATATTGCGGCTCTGCCAATAATAACGTCTTCTTCTTTAGTGTCCCAACTAGTCCTAGGAAAAGGACCATATCATCAACTTATGATGACTACCCAACACCACCCATGACACCATATCATGATGCCAATTCCGAACTCGGGGACTTTGAATTCGACACCAGTAACAGTTTCAATCTTGGTTCGGTTGATAATGTGACTAGCCAGCAGGTGAAAGACTTGTTTGATCGCAAGCAGCGCGAAAAAAGGCAGACCATGGCATTTGCTGATGACCTTTTCTGCGAGGGAAAAGTTATGCCGCTTGCACCTCCACTCAAACTTCCACCCCGTCTTGGTCAGAAATATACCAACCAAAGCCCGACCCCATCTACCCCAAGGTCACCTAGTTCTGGGATCAAAATGCGGGTTTTGCATAGGAGGTTGTGGAATGATGATTTTGATCCGTTCATGGTGGCGCTGCAGCATGTCAGGGAGGAGGAAAAGAGAGGGAAAGCTGAGACAGAGGCTCGTGACATATCTGACCTGAATGACCAACAGAATAAACAAACTGGCCTGCAAACACCAATTCACTCACCTTCTGTGAACTCCCCAAGTCTGCTACAAAGTCCAACCAGGCTGGCTCTGCCCAGAGGACTGGAGTTTGCAAGACAAGTTAGACTCGTCGGAATGGAATTGGATCATTCTGAGAGACACAGTCGCCCCAAATTGGAGACGGAGACGCCTAAGAATAACATTGCTCAAGAAAGTGGGGGGCCTTGTAAAAAGCAAACCAAGGGGCAGAAAATCAGAAGGCTTTTATTGAGTGCGTTGTTTGGGAAACCAGATGATGAAGACAAAAAGAGGAAAGATCAAACTTCTGAAATATTGAAAAAGCCAACATTTCTAAGGAGACTAAGCCTTAAGTCAGGAAGATCAACCAGCTGCAAAGCTGAGAAGAGGATATCTAACCAACTCACCAAGACAACGCTAGTACAATTCAGGCCAAGGCTTTTGCTCTGTATGGGTTATAGGGCAAGGTATCCACGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1645

Protein Analysis

360

Amino Acids

40.83

Weight (kDa)

9.66

Isoelectric Point (pI)

56.63

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0016253)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g06740
malus_domestica MD00G1045800.v1.1 MD03G1164300.v1.1
prunus_persica Prupe.4G260600_v2.0.a1
pyrus_communis pycom12561g00260
rosa_chinensis RchiOBHm_Chr6g0258931
rosa_laevigata RLG00000014570
rosa_multiflora Rmu_sc0004347.1_g000009
rosa_roxburghii Rroxscaffold_7G00206180
rosa_rugosa Rorug05G0584500
rosa_samantha Rh6AG100900 Rh6BG092800 Rh6CG090100 Rh6DG084300
rosa_wichuraiana Rw6G008760

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 49, 250
Acc36I ACCTGC 2 cut(s) 49, 250
AccII CGCG 1 cut(s) 291
AciI CCGC 3 cut(s) 63, 350, 454
AclWI GGATC 3 cut(s) 452, 485, 735
AcsI RAATTY 1 cut(s) 203
AcyI GRCGYC 1 cut(s) 770
AfaI GTAC 1 cut(s) 1023
AfiI CCNNNNNNNGG 4 cut(s) 192, 383, 440, 757
AflII CTTAAG 1 cut(s) 950
AgsI TTSAA 3 cut(s) 203, 226, 919
AhlI ACTAGT 1 cut(s) 104
AjnI CCWGG 2 cut(s) 49, 662
AluBI AGCT 3 cut(s) 545, 972, 980
AluI AGCT 3 cut(s) 545, 972, 980
Alw21I GWGCWC 1 cut(s) 40
Alw26I GTCTC 5 cut(s) 542, 733, 755, 761, 934
Alw44I GTGCAC 1 cut(s) 36
AlwI GGATC 3 cut(s) 452, 485, 735
Ama87I CYCGRG 1 cut(s) 191
AoxI GGCC 3 cut(s) 601, 803, 1031
ApaLI GTGCAC 1 cut(s) 36
ApeKI GCWGC 4 cut(s) 286, 508, 511, 972
ApoI RAATTY 1 cut(s) 203
ArsI GACNNNNNNTTYG 2 cut(s) 188, 220
AspA2I CCTAGG 1 cut(s) 110
AspLEI GCGC 2 cut(s) 291, 508
AspS9I GGNCC 2 cut(s) 120, 803
AsuHPI GGTGA 4 cut(s) 274, 423, 615, 997
AvaI CYCGRG 1 cut(s) 191
AvaII GGWCC 1 cut(s) 120
AvrII CCTAGG 1 cut(s) 110
BaeGI GKGCMC 1 cut(s) 40
BauI CACGAG 1 cut(s) 558
BbsI GAAGAC 2 cut(s) 76, 888
Bbv12I GWGCWC 1 cut(s) 40
BbvI GCAGC 4 cut(s) 298, 495, 523, 959
BccI CCATC 1 cut(s) 424
BciT130I CCWGG 2 cut(s) 51, 664
BciVI GTATCC 1 cut(s) 1080
BcoDI GTCTC 5 cut(s) 542, 733, 755, 761, 934
BcuI ACTAGT 1 cut(s) 104
BfaI CTAG 5 cut(s) 105, 111, 252, 435, 1019
BfmI CTRYAG 1 cut(s) 509
BfoI RGCGCY 1 cut(s) 509
BfrI CTTAAG 1 cut(s) 950
BfuAI ACCTGC 2 cut(s) 49, 250
BfuI GTATCC 1 cut(s) 1080
BisI GCNGC 6 cut(s) 64, 287, 350, 509, 512, 973
BlnI CCTAGG 1 cut(s) 110
BlsI GCNGC 6 cut(s) 65, 288, 351, 510, 513, 974
Bme1390I CCNGG 2 cut(s) 51, 664
Bme18I GGWCC 1 cut(s) 120
BmeT110I CYCGRG 1 cut(s) 191
BmgT120I GGNCC 2 cut(s) 120, 803
BmiI GGNNCC 1 cut(s) 804
BmrFI CCNGG 2 cut(s) 51, 664
BmsI GCATC 1 cut(s) 166
BpiI GAAGAC 2 cut(s) 76, 888
BpmI CTGGAG 1 cut(s) 707
BpuEI CTTGAG 1 cut(s) 774
BsaBI GATNNNNATC 1 cut(s) 489
BsaHI GRCGYC 1 cut(s) 770
BsaJI CCNNGG 7 cut(s) 49, 110, 306, 425, 822, 1034, 1073
Bsc4I CCNNNNNNNGG 4 cut(s) 192, 383, 440, 757
Bse1I ACTGG 3 cut(s) 213, 604, 690
Bse3DI GCAATG 1 cut(s) 783
Bse8I GATNNNNATC 1 cut(s) 489
BseBI CCWGG 2 cut(s) 51, 664
BseDI CCNNGG 7 cut(s) 49, 110, 306, 425, 822, 1034, 1073
BseJI GATNNNNATC 1 cut(s) 489
BseLI CCNNNNNNNGG 4 cut(s) 192, 383, 440, 757
BseMI GCAATG 1 cut(s) 783
BseMII CTCAG 3 cut(s) 537, 726, 972
BseNI ACTGG 3 cut(s) 213, 604, 690
BseRI GAGGAG 1 cut(s) 539
BseSI GKGCMC 1 cut(s) 40
BseXI GCAGC 4 cut(s) 298, 495, 523, 959
Bsh1236I CGCG 1 cut(s) 291
BshFI GGCC 3 cut(s) 603, 805, 1033
BsiHKAI GWGCWC 1 cut(s) 40
BsiHKCI CYCGRG 1 cut(s) 191
BslFI GGGAC 2 cut(s) 83, 209
BslI CCNNNNNNNGG 4 cut(s) 192, 383, 440, 757
BsmAI GTCTC 5 cut(s) 542, 733, 755, 761, 934
BsmBI CGTCTC 2 cut(s) 755, 761
BsmFI GGGAC 2 cut(s) 83, 209
BsnI GGCC 3 cut(s) 603, 805, 1033
BsoBI CYCGRG 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 40
Bsp143I GATC 6 cut(s) 277, 444, 490, 727, 898, 962
Bsp19I CCATGG 1 cut(s) 306
BspACI CCGC 3 cut(s) 63, 350, 454
BspANI GGCC 3 cut(s) 603, 805, 1033
BspCNI CTCAG 3 cut(s) 538, 727, 973
BspFNI CGCG 1 cut(s) 291
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 1 cut(s) 804
BspMAI CTGCAG 1 cut(s) 513
BspMI ACCTGC 2 cut(s) 49, 250
BspPI GGATC 3 cut(s) 452, 485, 735
BspTI CTTAAG 1 cut(s) 950
BsrDI GCAATG 1 cut(s) 783
BsrI ACTGG 3 cut(s) 213, 604, 690
BssECI CCNNGG 7 cut(s) 49, 110, 306, 425, 822, 1034, 1073
BssMI GATC 6 cut(s) 277, 444, 490, 727, 898, 962
BssNI GRCGYC 1 cut(s) 770
BssSI CACGAG 1 cut(s) 558
BssT1I CCWWGG 5 cut(s) 110, 306, 425, 822, 1034
Bst2BI CACGAG 1 cut(s) 558
Bst2UI CCWGG 2 cut(s) 51, 664
Bst4CI ACNGT 3 cut(s) 221, 746, 1077
Bst6I CTCTTC 1 cut(s) 980
BstACI GRCGYC 1 cut(s) 770
BstAFI CTTAAG 1 cut(s) 950
BstC8I GCNNGC 5 cut(s) 257, 284, 354, 605, 669
BstDEI CTNAG 6 cut(s) 546, 735, 774, 935, 944, 981
BstDSI CCRYGG 2 cut(s) 306, 1073
BstEII GGTNACC 1 cut(s) 429
BstFNI CGCG 1 cut(s) 291
BstH2I RGCGCY 1 cut(s) 509
BstHHI GCGC 2 cut(s) 291, 508
BstKTI GATC 6 cut(s) 280, 447, 493, 730, 901, 965
BstMAI GTCTC 5 cut(s) 542, 733, 755, 761, 934
BstMBI GATC 6 cut(s) 277, 444, 490, 727, 898, 962
BstMWI GCNNNNNNNGC 3 cut(s) 288, 460, 673
BstNI CCWGG 2 cut(s) 51, 664
BstNSI RCATGY 1 cut(s) 518
BstPI GGTNACC 1 cut(s) 429
BstSCI CCNGG 2 cut(s) 49, 662
BstSFI CTRYAG 1 cut(s) 509
BstSLI GKGCMC 1 cut(s) 40
BstUI CGCG 1 cut(s) 291
BstV1I GCAGC 4 cut(s) 298, 495, 523, 959
BstV2I GAAGAC 2 cut(s) 76, 888
BstXI CCANNNNNNTGG 1 cut(s) 685
BsuI GTATCC 1 cut(s) 1080
BsuRI GGCC 3 cut(s) 603, 805, 1033
BtgI CCRYGG 2 cut(s) 306, 1073
BtsIMutI CAGTG 1 cut(s) 40
BveI ACCTGC 2 cut(s) 49, 250
Cac8I GCNNGC 5 cut(s) 257, 284, 354, 605, 669
CciI TCATGA 1 cut(s) 172
CfoI GCGC 2 cut(s) 291, 508
Cfr13I GGNCC 2 cut(s) 120, 803
CseI GACGC 1 cut(s) 778
Csp6I GTAC 1 cut(s) 1022
CviAII CATG 5 cut(s) 160, 173, 307, 499, 515
CviQI GTAC 1 cut(s) 1022
DdeI CTNAG 6 cut(s) 546, 735, 774, 935, 944, 981
DpnI GATC 6 cut(s) 279, 446, 492, 729, 900, 964
DpnII GATC 6 cut(s) 277, 444, 490, 727, 898, 962
Eam1104I CTCTTC 1 cut(s) 980
EarI CTCTTC 1 cut(s) 980
Eco130I CCWWGG 5 cut(s) 110, 306, 425, 822, 1034
Eco32I GATATC 1 cut(s) 993
Eco47I GGWCC 1 cut(s) 120
Eco88I CYCGRG 1 cut(s) 191
Eco91I GGTNACC 1 cut(s) 429
EcoO109I RGGNCCY 1 cut(s) 803
EcoO65I GGTNACC 1 cut(s) 429
EcoRI GAATTC 1 cut(s) 203
EcoRII CCWGG 2 cut(s) 49, 662
EcoRV GATATC 1 cut(s) 993
EcoT14I CCWWGG 5 cut(s) 110, 306, 425, 822, 1034
ErhI CCWWGG 5 cut(s) 110, 306, 425, 822, 1034
Esp3I CGTCTC 2 cut(s) 755, 761
FaeI CATG 5 cut(s) 163, 176, 310, 502, 518
FaqI GGGAC 2 cut(s) 83, 209
FatI CATG 5 cut(s) 159, 172, 306, 498, 514
FauI CCCGC 1 cut(s) 447
Fnu4HI GCNGC 6 cut(s) 64, 287, 350, 509, 512, 973
Fsp4HI GCNGC 6 cut(s) 64, 287, 350, 509, 512, 973
FspBI CTAG 5 cut(s) 105, 111, 252, 435, 1019
GlaI GCGC 2 cut(s) 290, 507
GluI GCNGC 6 cut(s) 64, 287, 350, 509, 512, 973
GsuI CTGGAG 1 cut(s) 707
HaeII RGCGCY 1 cut(s) 509
HaeIII GGCC 3 cut(s) 603, 805, 1033
HgaI GACGC 1 cut(s) 778
HhaI GCGC 2 cut(s) 291, 508
Hin1I GRCGYC 1 cut(s) 770
Hin1II CATG 5 cut(s) 163, 176, 310, 502, 518
Hin6I GCGC 2 cut(s) 289, 506
HinP1I GCGC 2 cut(s) 289, 506
HinfI GANTC 1 cut(s) 708
HphI GGTGA 4 cut(s) 274, 423, 615, 997
Hpy166II GTNNAC 2 cut(s) 38, 634
Hpy188I TCNGA 7 cut(s) 187, 390, 571, 716, 736, 839, 910
Hpy188III TCNNGA 4 cut(s) 173, 560, 791, 957
Hpy8I GTNNAC 2 cut(s) 38, 634
Hpy99I CGWCG 1 cut(s) 716
HpyAV CCTTC 2 cut(s) 636, 834
HpyCH4III ACNGT 3 cut(s) 221, 746, 1077
HpyCH4IV ACGT 1 cut(s) 81
HpyCH4V TGCA 7 cut(s) 38, 356, 463, 511, 607, 695, 975
HpyF10VI GCNNNNNNNGC 3 cut(s) 288, 460, 673
HpyF3I CTNAG 6 cut(s) 546, 735, 774, 935, 944, 981
HpySE526I ACGT 1 cut(s) 81
Hsp92I GRCGYC 1 cut(s) 770
Hsp92II CATG 5 cut(s) 163, 176, 310, 502, 518
HspAI GCGC 2 cut(s) 289, 506
Kzo9I GATC 6 cut(s) 277, 444, 490, 727, 898, 962
LmnI GCTCC 1 cut(s) 29
Lsp1109I GCAGC 4 cut(s) 298, 495, 523, 959
LweI GCATC 1 cut(s) 166
MaeI CTAG 5 cut(s) 105, 111, 252, 435, 1019
MaeII ACGT 1 cut(s) 81
MaeIII GTNAC 4 cut(s) 215, 247, 429, 560
MalI GATC 6 cut(s) 279, 446, 492, 729, 900, 964
MboI GATC 6 cut(s) 277, 444, 490, 727, 898, 962
MboII GAAGA 6 cut(s) 66, 76, 79, 893, 972, 997
MhlI GDGCHC 1 cut(s) 40
MluCI AATT 6 cut(s) 181, 203, 615, 722, 755, 1025
MlyI GAGTC 1 cut(s) 702
MmeI TCCRAC 2 cut(s) 683, 694
MseI TTAA 1 cut(s) 951
MspA1I CMGCKG 1 cut(s) 972
MspCI CTTAAG 1 cut(s) 950
MspR9I CCNGG 2 cut(s) 51, 664
MvaI CCWGG 2 cut(s) 51, 664
MvnI CGCG 1 cut(s) 291
MwoI GCNNNNNNNGC 3 cut(s) 288, 460, 673
NcoI CCATGG 1 cut(s) 306
NdeII GATC 6 cut(s) 277, 444, 490, 727, 898, 962
NlaIII CATG 5 cut(s) 163, 176, 310, 502, 518
NlaIV GGNNCC 1 cut(s) 804
NmuCI GTSAC 3 cut(s) 247, 429, 560
NspI RCATGY 1 cut(s) 518
PagI TCATGA 1 cut(s) 172
PaqCI CACCTGC 2 cut(s) 49, 250
PflFI GACNNNGTC 1 cut(s) 744
PkrI GCNGC 6 cut(s) 65, 288, 351, 510, 513, 974
PleI GAGTC 1 cut(s) 702
PpsI GAGTC 1 cut(s) 702
Psp6I CCWGG 2 cut(s) 49, 662
PspEI GGTNACC 1 cut(s) 429
PspGI CCWGG 2 cut(s) 49, 662
PspN4I GGNNCC 1 cut(s) 804
PspPI GGNCC 2 cut(s) 120, 803
PstI CTGCAG 1 cut(s) 513
PsyI GACNNNGTC 1 cut(s) 744
PvuII CAGCTG 1 cut(s) 972
RsaI GTAC 1 cut(s) 1023
RsaNI GTAC 1 cut(s) 1022
SaqAI TTAA 1 cut(s) 951
SatI GCNGC 6 cut(s) 64, 287, 350, 509, 512, 973
Sau3AI GATC 6 cut(s) 277, 444, 490, 727, 898, 962
Sau96I GGNCC 2 cut(s) 120, 803
SchI GAGTC 1 cut(s) 702
ScrFI CCNGG 2 cut(s) 51, 664
SduI GDGCHC 1 cut(s) 40
SfaNI GCATC 1 cut(s) 166
SfcI CTRYAG 1 cut(s) 509
SinI GGWCC 1 cut(s) 120
SmlI CTYRAG 2 cut(s) 789, 950
SmoI CTYRAG 2 cut(s) 789, 950
SpeI ACTAGT 1 cut(s) 104
Sse9I AATT 6 cut(s) 181, 203, 615, 722, 755, 1025
SsiI CCGC 3 cut(s) 63, 350, 454
SspI AATATT 1 cut(s) 915
SspMI CTAG 5 cut(s) 105, 111, 252, 435, 1019
StyD4I CCNGG 2 cut(s) 49, 662
StyI CCWWGG 5 cut(s) 110, 306, 425, 822, 1034
TaaI ACNGT 3 cut(s) 221, 746, 1077
TaiI ACGT 1 cut(s) 84
TaqI TCGA 1 cut(s) 207
TasI AATT 6 cut(s) 181, 203, 615, 722, 755, 1025
TatI WGTACW 1 cut(s) 1021
TauI GCSGC 2 cut(s) 66, 352
Tru1I TTAA 1 cut(s) 951
Tru9I TTAA 1 cut(s) 951
TscAI CASTG 1 cut(s) 40
TseFI GTSAC 3 cut(s) 247, 429, 560
TseI GCWGC 4 cut(s) 286, 508, 511, 972
Tsp45I GTSAC 3 cut(s) 247, 429, 560
TspDTI ATGAA 2 cut(s) 487, 894
TspGWI ACGGA 2 cut(s) 483, 779
TspRI CASTG 1 cut(s) 40
Tth111I GACNNNGTC 1 cut(s) 744
Vha464I CTTAAG 1 cut(s) 950
VneI GTGCAC 1 cut(s) 36
VpaK11BI GGWCC 1 cut(s) 120
XapI RAATTY 1 cut(s) 203
XceI RCATGY 1 cut(s) 518
XmaJI CCTAGG 1 cut(s) 110
XspI CTAG 5 cut(s) 105, 111, 252, 435, 1019
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.