MD00G1048900.v1.1

Ganglioside-induced differentiation-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012918
Physical Location & Seq
Reverse (-)
9157991 .. 9161846
3856 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1048900.v1.1.491

Sequence Viewer

Length: 750 bp
ATGGGTAGCTCAGCCGCCGGAGAGGACTTCTCTGTGGTGGTTCTAGTGTCCGATCTGGGCATAGACGCGCGGCTGTTCTTGAACCACCAAGACAGAGAGATTGAAGAGCAAGAGAATTGGTACAACTGCCCTCAGAACCTTATCTCCGACGAGGACTTCTCCGACCTGGAGCTTTTGCAGTTCTTTCGTGTCCAAGGCCATGACAAGTTGGGAAATCGGATTTTCCGAATCTTAAAACCCCTTTGGCCTCCTCCAGTCGTGAGTGCAGATAGGTTGAAGAGGTGTATATTCCACAACTTACGCAGTGAGCTTCCAGAAGGGCCATACTCCATTGTTTACATGCATAGTACCGTGCAAAAGGAGGATAACTCCCCTGGCATAACTATCTTGAGGTGGCTTTATGAATACCTTCCATCATATTTGAAGGACATGCTTCAAGTTGTGTACTTCGTTCACCCTGGTCTCCAATCAAGACTTGTCTTTGCCACTCTTGGCCGATTTTTCTTAACTGGAGGCCTATATTGGAAAATCAAGTATGAAAGCCGCCTGCAGTACCTTTGGGATGACGTAAAGAAGGGAGAGGTTAAGATTCCTAAATTTGTAAAAAATCACGACGATGTTCTTGAGCATAGACCACTAACAGATTATGGCATAGAACCGAACCCTCTGCCCATGCTTGAGGTTCCGTCAACAGCCTACTCATTTGGAAGGTATGAGGAGAGATGGACATCCAGAGAGTACGTGTCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

250

Amino Acids

29.23

Weight (kDa)

5.55

Isoelectric Point (pI)

50.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 73 - 206 5.9e-25 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10210
fragaria_vesca FvH4_6g25120
malus_domestica MD00G1048900.v1.1 MD00G1074100.v1.1
prunus_persica Prupe.7G046200_v2.0.a1 Prupe.7G046200_v2.0.a1
pyrus_communis pycom12g07670
rosa_chinensis RchiOBHm_Chr2g0124961
rosa_laevigata RLG00000018840
rosa_multiflora Rmu_sc0008068.1_g000005
rosa_roxburghii Rroxscaffold_2G00118490 Rroxscaffold_2G00118790
rosa_rugosa Rorug02G0254500
rosa_samantha Rh2AG313100 Rh2BG321300 Rh2CG302600 Rh2DG338700
rosa_wichuraiana Rw2G025290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 68, 70
AciI CCGC 3 cut(s) 15, 70, 544
AcoI YGGCCR 1 cut(s) 493
AcsI RAATTY 1 cut(s) 596
AfaI GTAC 5 cut(s) 122, 349, 446, 554, 740
AflIII ACRYGT 1 cut(s) 741
AgsI TTSAA 5 cut(s) 82, 104, 277, 424, 437
AjnI CCWGG 3 cut(s) 165, 373, 457
AloI GAACNNNNNNTCC 2 cut(s) 128, 160
AluBI AGCT 3 cut(s) 9, 172, 310
AluI AGCT 3 cut(s) 9, 172, 310
Alw26I GTCTC 1 cut(s) 467
AoxI GGCC 5 cut(s) 196, 245, 320, 493, 514
ApoI RAATTY 1 cut(s) 596
Asp700I GAANNNNTTC 1 cut(s) 408
AspLEI GCGC 1 cut(s) 70
AspS9I GGNCC 1 cut(s) 320
AsuHPI GGTGA 1 cut(s) 446
BccI CCATC 2 cut(s) 421, 717
BcgI CGANNNNNNTGC 4 cut(s) 167, 201, 649, 683
BciT130I CCWGG 3 cut(s) 167, 375, 459
BcoDI GTCTC 1 cut(s) 467
BfaI CTAG 1 cut(s) 44
BfmI CTRYAG 1 cut(s) 548
BisI GCNGC 3 cut(s) 15, 71, 544
BlpI GCTNAGC 1 cut(s) 10
BlsI GCNGC 3 cut(s) 16, 72, 545
Bme1390I CCNGG 3 cut(s) 167, 375, 459
BmgT120I GGNCC 1 cut(s) 320
BmiI GGNNCC 1 cut(s) 684
BmrFI CCNGG 3 cut(s) 167, 375, 459
BplI GAGNNNNNCTC 6 cut(s) 14, 46, 143, 175, 353, 385
BpmI CTGGAG 3 cut(s) 188, 237, 531
Bpu1102I GCTNAGC 1 cut(s) 10
BpuEI CTTGAG 3 cut(s) 409, 644, 698
BsaAI YACGTR 1 cut(s) 742
BsaBI GATNNNNATC 1 cut(s) 727
BsaI GGTCTC 1 cut(s) 467
BsaJI CCNNGG 3 cut(s) 193, 373, 457
BsaXI ACNNNNNCTCC 2 cut(s) 128, 158
Bse1I ACTGG 2 cut(s) 254, 514
Bse8I GATNNNNATC 1 cut(s) 727
BseBI CCWGG 3 cut(s) 167, 375, 459
BseDI CCNNGG 3 cut(s) 193, 373, 457
BseGI GGATG 2 cut(s) 568, 728
BseJI GATNNNNATC 1 cut(s) 727
BseMII CTCAG 2 cut(s) 24, 146
BseNI ACTGG 2 cut(s) 254, 514
BseRI GAGGAG 2 cut(s) 240, 731
BsgI GTGCAG 1 cut(s) 285
Bsh1236I CGCG 2 cut(s) 68, 70
BshFI GGCC 5 cut(s) 198, 247, 322, 495, 516
BsiSI CCGG 1 cut(s) 18
BsmAI GTCTC 1 cut(s) 467
BsnI GGCC 5 cut(s) 198, 247, 322, 495, 516
Bso31I GGTCTC 1 cut(s) 467
Bsp143I GATC 1 cut(s) 52
Bsp1720I GCTNAGC 1 cut(s) 10
BspACI CCGC 3 cut(s) 15, 70, 544
BspANI GGCC 5 cut(s) 198, 247, 322, 495, 516
BspCNI CTCAG 2 cut(s) 23, 145
BspFNI CGCG 2 cut(s) 68, 70
BspLI GGNNCC 1 cut(s) 684
BspMAI CTGCAG 1 cut(s) 552
BspQI GCTCTTC 1 cut(s) 99
BspTNI GGTCTC 1 cut(s) 467
BsrI ACTGG 2 cut(s) 254, 514
BssECI CCNNGG 3 cut(s) 193, 373, 457
BssMI GATC 1 cut(s) 52
BssT1I CCWWGG 1 cut(s) 193
Bst2UI CCWGG 3 cut(s) 167, 375, 459
Bst4CI ACNGT 1 cut(s) 352
Bst6I CTCTTC 2 cut(s) 99, 272
BstBAI YACGTR 1 cut(s) 742
BstC8I GCNNGC 1 cut(s) 548
BstDEI CTNAG 2 cut(s) 10, 132
BstF5I GGATG 2 cut(s) 568, 728
BstFNI CGCG 2 cut(s) 68, 70
BstHHI GCGC 1 cut(s) 70
BstKTI GATC 1 cut(s) 55
BstMAI GTCTC 1 cut(s) 467
BstMBI GATC 1 cut(s) 52
BstNI CCWGG 3 cut(s) 167, 375, 459
BstNSI RCATGY 2 cut(s) 343, 433
BstSCI CCNGG 3 cut(s) 165, 373, 457
BstSFI CTRYAG 1 cut(s) 548
BstUI CGCG 2 cut(s) 68, 70
BsuRI GGCC 5 cut(s) 198, 247, 322, 495, 516
BtsCI GGATG 2 cut(s) 568, 728
BtsI GCAGTG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 310
Cac8I GCNNGC 1 cut(s) 548
CfoI GCGC 1 cut(s) 70
Cfr13I GGNCC 1 cut(s) 320
CseI GACGC 1 cut(s) 74
Csp6I GTAC 5 cut(s) 121, 348, 445, 553, 739
CviAII CATG 4 cut(s) 200, 340, 430, 673
CviQI GTAC 5 cut(s) 121, 348, 445, 553, 739
DdeI CTNAG 2 cut(s) 10, 132
DpnI GATC 1 cut(s) 54
DpnII GATC 1 cut(s) 52
EaeI YGGCCR 1 cut(s) 493
Eam1104I CTCTTC 2 cut(s) 99, 272
EarI CTCTTC 2 cut(s) 99, 272
Eco130I CCWWGG 1 cut(s) 193
Eco147I AGGCCT 1 cut(s) 516
Eco31I GGTCTC 1 cut(s) 467
EcoRII CCWGG 3 cut(s) 165, 373, 457
EcoT14I CCWWGG 1 cut(s) 193
EcoT22I ATGCAT 1 cut(s) 345
ErhI CCWWGG 1 cut(s) 193
FaeI CATG 4 cut(s) 203, 343, 433, 676
FatI CATG 4 cut(s) 199, 339, 429, 672
Fnu4HI GCNGC 3 cut(s) 15, 71, 544
FokI GGATG 2 cut(s) 575, 715
Fsp4HI GCNGC 3 cut(s) 15, 71, 544
FspBI CTAG 1 cut(s) 44
GlaI GCGC 1 cut(s) 69
GluI GCNGC 3 cut(s) 15, 71, 544
GsuI CTGGAG 3 cut(s) 188, 237, 531
HaeIII GGCC 5 cut(s) 198, 247, 322, 495, 516
HapII CCGG 1 cut(s) 18
HgaI GACGC 1 cut(s) 74
HhaI GCGC 1 cut(s) 70
Hin1II CATG 4 cut(s) 203, 343, 433, 676
Hin6I GCGC 1 cut(s) 68
HinP1I GCGC 1 cut(s) 68
HincII GTYRAC 1 cut(s) 690
HindII GTYRAC 1 cut(s) 690
HinfI GANTC 2 cut(s) 228, 589
HpaII CCGG 1 cut(s) 18
HphI GGTGA 1 cut(s) 446
Hpy166II GTNNAC 4 cut(s) 337, 445, 454, 690
Hpy188I TCNGA 6 cut(s) 52, 135, 148, 163, 219, 227
Hpy188III TCNNGA 8 cut(s) 79, 259, 314, 388, 471, 611, 623, 732
Hpy8I GTNNAC 4 cut(s) 337, 445, 454, 690
Hpy99I CGWCG 2 cut(s) 152, 617
HpyAV CCTTC 5 cut(s) 311, 418, 419, 568, 702
HpyCH4III ACNGT 1 cut(s) 352
HpyCH4IV ACGT 2 cut(s) 567, 741
HpyCH4V TGCA 5 cut(s) 178, 266, 343, 355, 550
HpyF3I CTNAG 2 cut(s) 10, 132
HpySE526I ACGT 2 cut(s) 567, 741
Hsp92II CATG 4 cut(s) 203, 343, 433, 676
HspAI GCGC 1 cut(s) 68
Kzo9I GATC 1 cut(s) 52
LguI GCTCTTC 1 cut(s) 99
LmnI GCTCC 1 cut(s) 169
MaeI CTAG 1 cut(s) 44
MaeII ACGT 2 cut(s) 567, 741
MalI GATC 1 cut(s) 54
MboI GATC 1 cut(s) 52
MboII GAAGA 2 cut(s) 116, 289
MluCI AATT 2 cut(s) 115, 596
MmeI TCCRAC 2 cut(s) 171, 186
Mph1103I ATGCAT 1 cut(s) 345
MroXI GAANNNNTTC 1 cut(s) 408
MseI TTAA 3 cut(s) 233, 506, 585
MslI CAYNNNNRTG 1 cut(s) 615
MspI CCGG 1 cut(s) 18
MspR9I CCNGG 3 cut(s) 167, 375, 459
MvaI CCWGG 3 cut(s) 167, 375, 459
MvnI CGCG 2 cut(s) 68, 70
NdeII GATC 1 cut(s) 52
NlaIII CATG 4 cut(s) 203, 343, 433, 676
NlaIV GGNNCC 1 cut(s) 684
NsiI ATGCAT 1 cut(s) 345
NspI RCATGY 2 cut(s) 343, 433
PceI AGGCCT 1 cut(s) 516
PciSI GCTCTTC 1 cut(s) 99
PcsI WCGNNNNNNNCGW 1 cut(s) 223
PdmI GAANNNNTTC 1 cut(s) 408
PfeI GAWTC 2 cut(s) 228, 589
PkrI GCNGC 3 cut(s) 16, 72, 545
Ppu21I YACGTR 1 cut(s) 742
Psp6I CCWGG 3 cut(s) 165, 373, 457
PspGI CCWGG 3 cut(s) 165, 373, 457
PspN4I GGNNCC 1 cut(s) 684
PspPI GGNCC 1 cut(s) 320
PstI CTGCAG 1 cut(s) 552
RsaI GTAC 5 cut(s) 122, 349, 446, 554, 740
RsaNI GTAC 5 cut(s) 121, 348, 445, 553, 739
RseI CAYNNNNRTG 1 cut(s) 615
SapI GCTCTTC 1 cut(s) 99
SaqAI TTAA 3 cut(s) 233, 506, 585
SatI GCNGC 3 cut(s) 15, 71, 544
Sau3AI GATC 1 cut(s) 52
Sau96I GGNCC 1 cut(s) 320
ScrFI CCNGG 3 cut(s) 167, 375, 459
SfcI CTRYAG 1 cut(s) 548
SmiMI CAYNNNNRTG 1 cut(s) 615
SmlI CTYRAG 3 cut(s) 388, 623, 677
SmoI CTYRAG 3 cut(s) 388, 623, 677
Sse9I AATT 2 cut(s) 115, 596
SseBI AGGCCT 1 cut(s) 516
SsiI CCGC 3 cut(s) 15, 70, 544
SspMI CTAG 1 cut(s) 44
StuI AGGCCT 1 cut(s) 516
StyD4I CCNGG 3 cut(s) 165, 373, 457
StyI CCWWGG 1 cut(s) 193
TaaI ACNGT 1 cut(s) 352
TaiI ACGT 2 cut(s) 570, 744
TasI AATT 2 cut(s) 115, 596
TatI WGTACW 1 cut(s) 444
TauI GCSGC 3 cut(s) 17, 73, 546
TfiI GAWTC 2 cut(s) 228, 589
Tru1I TTAA 3 cut(s) 233, 506, 585
Tru9I TTAA 3 cut(s) 233, 506, 585
TscAI CASTG 1 cut(s) 310
TspDTI ATGAA 2 cut(s) 417, 552
TspGWI ACGGA 1 cut(s) 675
TspRI CASTG 1 cut(s) 310
XapI RAATTY 1 cut(s) 596
XceI RCATGY 2 cut(s) 343, 433
XmnI GAANNNNTTC 1 cut(s) 408
XspI CTAG 1 cut(s) 44
Zsp2I ATGCAT 1 cut(s) 345
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.