MD00G1074100.v1.1

Ganglioside-induced differentiation-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0012918
Physical Location & Seq
Reverse (-)
14927459 .. 14930559
3101 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1074100.v1.1.491

Sequence Viewer

Length: 942 bp
ATGCCACTCCTTCCAGCCAGTATTCACGATCATCCATCTGCTGTAAAAAAATTCTCGAATCACTTTATCACATCACGCCACGTGTCCAATCTCACCGAGGAGGTTCCACACAGAAGCGCAAACAGAAAGAGAAATAAAATGCGAGAAAAGAGAGAGTGCGGGAAAGAGAGAGAGAACCAAGAAGAATCAGATATGGGTAGTTCAGCCGACGGAGAGGATTTCTCAGTGGTCGTTCTGGCGTCCGATCTGGGCGTAGACGCGCGGCCGTTCTTGGCGAACCAAGACAGAGAGATTGAAGAGCAAGAGAATTGGTACGACTGCCCTCAGAACCTTATCTCCGACGAGGACTTCTCTGATCTCGAGCTTTTGCAGTTCTTTAGTGTCGAGGGCCAGGACAAGTCTGGAAATCGTATTTTCCGCATCGTCGGAAAATATTTTCCCGCTCCAGTTGTTAGTGCAGACCGGCTGAAGAGGTATATATTCCACAAATTATGCAGTGAGGTCCCAGAAGGGCCATACTCCGTTGTTTACATGCATAGTACTGTGCAAAAGGAAGATAACTCCCCTGGCATAACTATCTTGCGGTGGCTTTATGAAGACCTTCCATCATACTTGAAGGACAGGCTTCAAGTTGTGTACTTCGTTCACCCTGGGCTCCGATCAAGACTTGTCTTTGCCACTCTTGGCCGATTTTTCTTAACTGGAGGCCTATATTGGAAAATCAAGTATGTAAGCCGGCTGCAGTACCTATGGGATGATATAAAGAAAGGAGAGGTTGAGATTCCTGAATTTGTCAAAAATCATGACGATGTTCTTGAGCATAGACCACTAACAGATTATGGCATTGAACCGGACCCTCTTCACATGCTTGAGGCTCCATCAACAGCCTACTCATTTGGAAGGTATGAGGAGAGATGGACGTCAAGAGAGTATACATCGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

314

Amino Acids

36.48

Weight (kDa)

5.53

Isoelectric Point (pI)

56.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 135 - 270 8.6e-33 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10210
fragaria_vesca FvH4_6g25120
malus_domestica MD00G1048900.v1.1 MD00G1074100.v1.1
prunus_persica Prupe.7G046200_v2.0.a1 Prupe.7G046200_v2.0.a1
pyrus_communis pycom12g07670
rosa_chinensis RchiOBHm_Chr2g0124961
rosa_laevigata RLG00000018840
rosa_multiflora Rmu_sc0008068.1_g000005
rosa_roxburghii Rroxscaffold_2G00118490 Rroxscaffold_2G00118790
rosa_rugosa Rorug02G0254500
rosa_samantha Rh2AG313100 Rh2BG321300 Rh2CG302600 Rh2DG338700
rosa_wichuraiana Rw2G025290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 923
AccBSI CCGCTC 1 cut(s) 443
AccI GTMKAC 2 cut(s) 255, 932
AccII CGCG 2 cut(s) 260, 262
AciI CCGC 5 cut(s) 159, 262, 418, 441, 583
AcoI YGGCCR 2 cut(s) 263, 685
AcsI RAATTY 2 cut(s) 50, 788
AcuI CTGAAG 1 cut(s) 488
AcvI CACGTG 1 cut(s) 82
AcyI GRCGYC 2 cut(s) 239, 920
AfaI GTAC 4 cut(s) 314, 541, 638, 746
AflIII ACRYGT 1 cut(s) 81
AgsI TTSAA 4 cut(s) 296, 616, 629, 848
AjnI CCWGG 3 cut(s) 390, 565, 649
AloI GAACNNNNNNTCC 2 cut(s) 320, 352
AluBI AGCT 1 cut(s) 364
AluI AGCT 1 cut(s) 364
Ama87I CYCGRG 1 cut(s) 359
AoxI GGCC 5 cut(s) 263, 388, 512, 685, 706
ApeKI GCWGC 1 cut(s) 739
ApoI RAATTY 2 cut(s) 50, 788
Asp700I GAANNNNTTC 1 cut(s) 600
AspLEI GCGC 2 cut(s) 119, 262
AspS9I GGNCC 4 cut(s) 388, 502, 512, 853
AsuHPI GGTGA 2 cut(s) 85, 638
AvaI CYCGRG 1 cut(s) 359
AvaII GGWCC 2 cut(s) 502, 853
BanII GRGCYC 1 cut(s) 657
BbrPI CACGTG 1 cut(s) 82
BbsI GAAGAC 1 cut(s) 603
BbvI GCAGC 1 cut(s) 726
BccI CCATC 4 cut(s) 43, 613, 886, 909
BceAI ACGGC 1 cut(s) 250
BciT130I CCWGG 3 cut(s) 392, 567, 651
BfmI CTRYAG 1 cut(s) 740
BisI GCNGC 2 cut(s) 263, 740
BlsI GCNGC 2 cut(s) 264, 741
BmcAI AGTACT 1 cut(s) 541
Bme1390I CCNGG 3 cut(s) 392, 567, 651
Bme18I GGWCC 2 cut(s) 502, 853
BmeT110I CYCGRG 1 cut(s) 359
BmgT120I GGNCC 4 cut(s) 388, 502, 512, 853
BmiI GGNNCC 5 cut(s) 105, 504, 656, 855, 876
BmrFI CCNGG 3 cut(s) 392, 567, 651
BmsI GCATC 1 cut(s) 429
BpiI GAAGAC 1 cut(s) 603
BplI GAGNNNNNCTC 4 cut(s) 206, 238, 335, 367
BpmI CTGGAG 2 cut(s) 429, 723
BpuEI CTTGAG 2 cut(s) 836, 890
BsaAI YACGTR 1 cut(s) 82
BsaHI GRCGYC 2 cut(s) 239, 920
BsaJI CCNNGG 4 cut(s) 96, 565, 649, 650
BsaWI WCCGGW 1 cut(s) 850
BsaXI ACNNNNNCTCC 2 cut(s) 320, 350
Bse118I RCCGGY 2 cut(s) 462, 735
Bse1I ACTGG 3 cut(s) 18, 446, 706
BseBI CCWGG 3 cut(s) 392, 567, 651
BseDI CCNNGG 4 cut(s) 96, 565, 649, 650
BseGI GGATG 2 cut(s) 31, 760
BseMII CTCAG 2 cut(s) 237, 338
BseNI ACTGG 3 cut(s) 18, 446, 706
BseRI GAGGAG 2 cut(s) 113, 923
BseX3I CGGCCG 1 cut(s) 263
BseXI GCAGC 1 cut(s) 726
BsgI GTGCAG 1 cut(s) 477
Bsh1236I CGCG 2 cut(s) 260, 262
Bsh1285I CGRYCG 1 cut(s) 266
BshFI GGCC 5 cut(s) 265, 390, 514, 687, 708
BsiEI CGRYCG 1 cut(s) 266
BsiHKCI CYCGRG 1 cut(s) 359
BsiSI CCGG 3 cut(s) 463, 736, 851
BslFI GGGAC 1 cut(s) 488
BsmFI GGGAC 1 cut(s) 488
BsnI GGCC 5 cut(s) 265, 390, 514, 687, 708
BsoBI CYCGRG 1 cut(s) 359
Bsp1286I GDGCHC 1 cut(s) 657
Bsp143I GATC 4 cut(s) 28, 244, 355, 659
BspACI CCGC 5 cut(s) 159, 262, 418, 441, 583
BspANI GGCC 5 cut(s) 265, 390, 514, 687, 708
BspCNI CTCAG 2 cut(s) 236, 337
BspFNI CGCG 2 cut(s) 260, 262
BspHI TCATGA 1 cut(s) 802
BspLI GGNNCC 5 cut(s) 105, 504, 656, 855, 876
BspMAI CTGCAG 1 cut(s) 744
BspQI GCTCTTC 1 cut(s) 291
BsrBI CCGCTC 1 cut(s) 443
BsrFI RCCGGY 2 cut(s) 462, 735
BsrI ACTGG 3 cut(s) 18, 446, 706
BssAI RCCGGY 2 cut(s) 462, 735
BssECI CCNNGG 4 cut(s) 96, 565, 649, 650
BssMI GATC 4 cut(s) 28, 244, 355, 659
BssNAI GTATAC 1 cut(s) 933
BssNI GRCGYC 2 cut(s) 239, 920
Bst1107I GTATAC 1 cut(s) 933
Bst2UI CCWGG 3 cut(s) 392, 567, 651
Bst4CI ACNGT 1 cut(s) 544
Bst6I CTCTTC 3 cut(s) 291, 464, 864
BstACI GRCGYC 2 cut(s) 239, 920
BstBAI YACGTR 1 cut(s) 82
BstC8I GCNNGC 1 cut(s) 737
BstDEI CTNAG 2 cut(s) 223, 324
BstF5I GGATG 2 cut(s) 31, 760
BstFNI CGCG 2 cut(s) 260, 262
BstHHI GCGC 2 cut(s) 119, 262
BstKTI GATC 4 cut(s) 31, 247, 358, 662
BstMBI GATC 4 cut(s) 28, 244, 355, 659
BstMCI CGRYCG 1 cut(s) 266
BstNI CCWGG 3 cut(s) 392, 567, 651
BstNSI RCATGY 2 cut(s) 535, 868
BstSCI CCNGG 3 cut(s) 390, 565, 649
BstSFI CTRYAG 1 cut(s) 740
BstUI CGCG 2 cut(s) 260, 262
BstV1I GCAGC 1 cut(s) 726
BstV2I GAAGAC 1 cut(s) 603
BstZ17I GTATAC 1 cut(s) 933
BstZI CGGCCG 1 cut(s) 263
BsuRI GGCC 5 cut(s) 265, 390, 514, 687, 708
BtsCI GGATG 2 cut(s) 31, 760
BtsI GCAGTG 1 cut(s) 502
BtsIMutI CAGTG 2 cut(s) 231, 502
Cac8I GCNNGC 1 cut(s) 737
CciI TCATGA 1 cut(s) 802
CfoI GCGC 2 cut(s) 119, 262
Cfr10I RCCGGY 2 cut(s) 462, 735
Cfr13I GGNCC 4 cut(s) 388, 502, 512, 853
CseI GACGC 2 cut(s) 228, 266
Csp6I GTAC 4 cut(s) 313, 540, 637, 745
CviAII CATG 3 cut(s) 532, 803, 865
CviQI GTAC 4 cut(s) 313, 540, 637, 745
DdeI CTNAG 2 cut(s) 223, 324
DpnI GATC 4 cut(s) 30, 246, 357, 661
DpnII GATC 4 cut(s) 28, 244, 355, 659
EaeI YGGCCR 2 cut(s) 263, 685
EagI CGGCCG 1 cut(s) 263
Eam1104I CTCTTC 3 cut(s) 291, 464, 864
EarI CTCTTC 3 cut(s) 291, 464, 864
EclXI CGGCCG 1 cut(s) 263
Eco147I AGGCCT 1 cut(s) 708
Eco24I GRGCYC 1 cut(s) 657
Eco47I GGWCC 2 cut(s) 502, 853
Eco52I CGGCCG 1 cut(s) 263
Eco57I CTGAAG 1 cut(s) 488
Eco72I CACGTG 1 cut(s) 82
Eco88I CYCGRG 1 cut(s) 359
EcoO109I RGGNCCY 1 cut(s) 502
EcoRII CCWGG 3 cut(s) 390, 565, 649
EcoT22I ATGCAT 1 cut(s) 537
EcoT38I GRGCYC 1 cut(s) 657
FaeI CATG 3 cut(s) 535, 806, 868
FaqI GGGAC 1 cut(s) 488
FatI CATG 3 cut(s) 531, 802, 864
FauI CCCGC 2 cut(s) 152, 448
FblI GTMKAC 2 cut(s) 255, 932
Fnu4HI GCNGC 2 cut(s) 263, 740
FokI GGATG 2 cut(s) 18, 767
FriOI GRGCYC 1 cut(s) 657
Fsp4HI GCNGC 2 cut(s) 263, 740
GlaI GCGC 2 cut(s) 118, 261
GluI GCNGC 2 cut(s) 263, 740
GsuI CTGGAG 2 cut(s) 429, 723
HaeIII GGCC 5 cut(s) 265, 390, 514, 687, 708
HapII CCGG 3 cut(s) 463, 736, 851
HgaI GACGC 2 cut(s) 228, 266
HhaI GCGC 2 cut(s) 119, 262
Hin1I GRCGYC 2 cut(s) 239, 920
Hin1II CATG 3 cut(s) 535, 806, 868
Hin6I GCGC 2 cut(s) 117, 260
HinP1I GCGC 2 cut(s) 117, 260
HinfI GANTC 3 cut(s) 58, 185, 781
HpaII CCGG 3 cut(s) 463, 736, 851
HphI GGTGA 2 cut(s) 85, 638
Hpy166II GTNNAC 5 cut(s) 256, 529, 637, 646, 933
Hpy188I TCNGA 7 cut(s) 190, 244, 327, 340, 355, 428, 659
Hpy188III TCNNGA 9 cut(s) 26, 55, 359, 402, 663, 785, 803, 815, 924
Hpy8I GTNNAC 5 cut(s) 256, 529, 637, 646, 933
Hpy99I CGWCG 3 cut(s) 212, 344, 428
HpyAV CCTTC 5 cut(s) 20, 503, 610, 611, 894
HpyCH4III ACNGT 1 cut(s) 544
HpyCH4IV ACGT 2 cut(s) 81, 920
HpyCH4V TGCA 6 cut(s) 370, 458, 495, 535, 547, 742
HpyF3I CTNAG 2 cut(s) 223, 324
HpySE526I ACGT 2 cut(s) 81, 920
Hsp92I GRCGYC 2 cut(s) 239, 920
Hsp92II CATG 3 cut(s) 535, 806, 868
HspAI GCGC 2 cut(s) 117, 260
KroI GCCGGC 1 cut(s) 735
KroNI GCCGGC 1 cut(s) 737
Kzo9I GATC 4 cut(s) 28, 244, 355, 659
LguI GCTCTTC 1 cut(s) 291
LmnI GCTCC 3 cut(s) 448, 660, 880
Lsp1109I GCAGC 1 cut(s) 726
LweI GCATC 1 cut(s) 429
MaeII ACGT 2 cut(s) 81, 920
MalI GATC 4 cut(s) 30, 246, 357, 661
MbiI CCGCTC 1 cut(s) 443
MboI GATC 4 cut(s) 28, 244, 355, 659
MboII GAAGA 6 cut(s) 194, 308, 481, 566, 608, 851
MhlI GDGCHC 1 cut(s) 657
MluCI AATT 4 cut(s) 50, 307, 488, 788
MmeI TCCRAC 2 cut(s) 363, 406
Mph1103I ATGCAT 1 cut(s) 537
MroNI GCCGGC 1 cut(s) 735
MroXI GAANNNNTTC 1 cut(s) 600
MseI TTAA 1 cut(s) 698
MslI CAYNNNNRTG 1 cut(s) 807
MspI CCGG 3 cut(s) 463, 736, 851
MspR9I CCNGG 3 cut(s) 392, 567, 651
MvaI CCWGG 3 cut(s) 392, 567, 651
MvnI CGCG 2 cut(s) 260, 262
NaeI GCCGGC 1 cut(s) 737
NdeII GATC 4 cut(s) 28, 244, 355, 659
NgoMIV GCCGGC 1 cut(s) 735
NlaIII CATG 3 cut(s) 535, 806, 868
NlaIV GGNNCC 5 cut(s) 105, 504, 656, 855, 876
NsiI ATGCAT 1 cut(s) 537
NspI RCATGY 2 cut(s) 535, 868
PaeR7I CTCGAG 1 cut(s) 359
PagI TCATGA 1 cut(s) 802
PasI CCCWGGG 1 cut(s) 650
PceI AGGCCT 1 cut(s) 708
PciSI GCTCTTC 1 cut(s) 291
PdiI GCCGGC 1 cut(s) 737
PdmI GAANNNNTTC 1 cut(s) 600
PfeI GAWTC 3 cut(s) 58, 185, 781
PkrI GCNGC 2 cut(s) 264, 741
PmaCI CACGTG 1 cut(s) 82
PmlI CACGTG 1 cut(s) 82
Ppu21I YACGTR 1 cut(s) 82
PpuMI RGGWCCY 1 cut(s) 502
Psp5II RGGWCCY 1 cut(s) 502
Psp6I CCWGG 3 cut(s) 390, 565, 649
PspCI CACGTG 1 cut(s) 82
PspGI CCWGG 3 cut(s) 390, 565, 649
PspN4I GGNNCC 5 cut(s) 105, 504, 656, 855, 876
PspPI GGNCC 4 cut(s) 388, 502, 512, 853
PspPPI RGGWCCY 1 cut(s) 502
PstI CTGCAG 1 cut(s) 744
RsaI GTAC 4 cut(s) 314, 541, 638, 746
RsaNI GTAC 4 cut(s) 313, 540, 637, 745
RseI CAYNNNNRTG 1 cut(s) 807
SapI GCTCTTC 1 cut(s) 291
SaqAI TTAA 1 cut(s) 698
SatI GCNGC 2 cut(s) 263, 740
Sau3AI GATC 4 cut(s) 28, 244, 355, 659
Sau96I GGNCC 4 cut(s) 388, 502, 512, 853
ScaI AGTACT 1 cut(s) 541
ScrFI CCNGG 3 cut(s) 392, 567, 651
SduI GDGCHC 1 cut(s) 657
SfaNI GCATC 1 cut(s) 429
SfcI CTRYAG 1 cut(s) 740
Sfr274I CTCGAG 1 cut(s) 359
SinI GGWCC 2 cut(s) 502, 853
SlaI CTCGAG 1 cut(s) 359
SmiMI CAYNNNNRTG 1 cut(s) 807
SmlI CTYRAG 3 cut(s) 359, 815, 869
SmoI CTYRAG 3 cut(s) 359, 815, 869
Sse9I AATT 4 cut(s) 50, 307, 488, 788
SseBI AGGCCT 1 cut(s) 708
SsiI CCGC 5 cut(s) 159, 262, 418, 441, 583
SspI AATATT 1 cut(s) 434
StuI AGGCCT 1 cut(s) 708
StyD4I CCNGG 3 cut(s) 390, 565, 649
TaaI ACNGT 1 cut(s) 544
TaiI ACGT 2 cut(s) 84, 923
TaqI TCGA 3 cut(s) 56, 360, 384
TasI AATT 4 cut(s) 50, 307, 488, 788
TatI WGTACW 2 cut(s) 539, 636
TauI GCSGC 1 cut(s) 265
TfiI GAWTC 3 cut(s) 58, 185, 781
Tru1I TTAA 1 cut(s) 698
Tru9I TTAA 1 cut(s) 698
TscAI CASTG 2 cut(s) 231, 502
TseI GCWGC 1 cut(s) 739
TspDTI ATGAA 1 cut(s) 609
TspGWI ACGGA 2 cut(s) 225, 511
TspRI CASTG 2 cut(s) 231, 502
VpaK11BI GGWCC 2 cut(s) 502, 853
XapI RAATTY 2 cut(s) 50, 788
XceI RCATGY 2 cut(s) 535, 868
XcmI CCANNNNNNNNNTGG 1 cut(s) 398
XhoI CTCGAG 1 cut(s) 359
XmiI GTMKAC 2 cut(s) 255, 932
XmnI GAANNNNTTC 1 cut(s) 600
ZraI GACGTC 1 cut(s) 921
ZrmI AGTACT 1 cut(s) 541
Zsp2I ATGCAT 1 cut(s) 537
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.