RLG00000018840

Ganglioside-induced differentiation-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr4
OG0012918
Physical Location & Seq
Reverse (-)
37598156 .. 37600349
2194 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000018840

Sequence Viewer

Length: 762 bp
ATGGGGAGCTCACCAAACGACTTCTCGGTGGTGGTACTGCCATCGGACTATGGAATCGACGCGAGGCCGTTCCTCACGAACCAGCAGAGAGTGTCCTCACAAGAAGATGAAGAAGAAGACCAAGACAATTGGCACGACTGTCCTCAGCACCTCCCTTCCGACGAGGACTTCTCCGATCTCGAGCTCTTGCAGTTCTTTCGTCCCCAAGGCCGTGACAAATCTGGAAGCCAGATTTTCCGTATTGTCGGAAAATACTTTCCCGCTCCAGTTGTGAGTGGGGATAGGCTAAAGAGGTACATATGCAACAAACTATGCAGTGACTCTCCAGAAGGGCCATATTGCATTGTTTACATGCATAGTACTGTGCAAAAGGAGGATAACTCCCCTGGAATGACTATCTTGAGGTGGATTTATGAAGAACTTCCTAGTTATTTGAAGGATAGGCTCCAAGTTGTGTACTTCATTCACCCTGGACTTCGCTCAAGGCTTGTCTTTGCCACTCTTGGCCGATTTTTCTTAAGTGGAGGCCTATATTGGAAAATCAAGTATGTAAGCCGCCTGCAGTATCTTTGGGATGATATAAAGAAAGGAGAGATTGAGATTCCCGACTTTGTAAAAAATCATGATGATGTTCTTGAGCATAGACCATTAACAGATTATGGGATCGAACCAGACCCTCTTCACATGATTGAGGTGCCATCAACTGCATATTCATTTGGAAGGTATGAGGAGAGATGGGCATCAAGAGAATATACGTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CRAL_TRIO_2

Protein Analysis

254

Amino Acids

29.55

Weight (kDa)

5.04

Isoelectric Point (pI)

62.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CRAL_TRIO_2 PF13716 75 - 210 1.2e-31 Divergent CRAL/TRIO domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012918)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G10210
fragaria_vesca FvH4_6g25120
malus_domestica MD00G1048900.v1.1 MD00G1074100.v1.1
prunus_persica Prupe.7G046200_v2.0.a1 Prupe.7G046200_v2.0.a1
pyrus_communis pycom12g07670
rosa_chinensis RchiOBHm_Chr2g0124961
rosa_laevigata RLG00000018840
rosa_multiflora Rmu_sc0008068.1_g000005
rosa_roxburghii Rroxscaffold_2G00118490 Rroxscaffold_2G00118790
rosa_rugosa Rorug02G0254500
rosa_samantha Rh2AG313100 Rh2BG321300 Rh2CG302600 Rh2DG338700
rosa_wichuraiana Rw2G025290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 694
AccBSI CCGCTC 1 cut(s) 263
AccII CGCG 1 cut(s) 62
AciI CCGC 2 cut(s) 261, 556
AclWI GGATC 1 cut(s) 671
AcoI YGGCCR 1 cut(s) 505
AfaI GTAC 4 cut(s) 36, 296, 361, 458
AflII CTTAAG 1 cut(s) 517
AgsI TTSAA 1 cut(s) 436
AjnI CCWGG 2 cut(s) 385, 469
AluBI AGCT 2 cut(s) 9, 184
AluI AGCT 2 cut(s) 9, 184
Alw21I GWGCWC 2 cut(s) 11, 186
AlwI GGATC 1 cut(s) 671
Ama87I CYCGRG 1 cut(s) 179
AoxI GGCC 5 cut(s) 65, 208, 332, 505, 526
Asp700I GAANNNNTTC 1 cut(s) 420
AspS9I GGNCC 1 cut(s) 332
AsuHPI GGTGA 2 cut(s) 3, 458
AvaI CYCGRG 1 cut(s) 179
BanI GGYRCC 1 cut(s) 694
BanII GRGCYC 2 cut(s) 11, 186
BbsI GAAGAC 1 cut(s) 123
Bbv12I GWGCWC 2 cut(s) 11, 186
BbvCI CCTCAGC 1 cut(s) 144
BccI CCATC 3 cut(s) 49, 706, 729
BceAI ACGGC 2 cut(s) 52, 195
BcgI CGANNNNNNTGC 2 cut(s) 179, 213
BciT130I CCWGG 2 cut(s) 387, 471
BfaI CTAG 1 cut(s) 426
BfmI CTRYAG 1 cut(s) 560
BfrI CTTAAG 1 cut(s) 517
BisI GCNGC 1 cut(s) 556
BlsI GCNGC 1 cut(s) 557
BmcAI AGTACT 1 cut(s) 361
Bme1390I CCNGG 2 cut(s) 387, 471
BmeT110I CYCGRG 1 cut(s) 179
BmgT120I GGNCC 1 cut(s) 332
BmiI GGNNCC 2 cut(s) 446, 696
BmrFI CCNGG 2 cut(s) 387, 471
BmsI GCATC 1 cut(s) 749
BpiI GAAGAC 1 cut(s) 123
BplI GAGNNNNNCTC 4 cut(s) 155, 187, 365, 397
BpmI CTGGAG 2 cut(s) 249, 309
Bpu10I CCTNAGC 1 cut(s) 144
BpuEI CTTGAG 3 cut(s) 421, 466, 656
BsaBI GATNNNNATC 1 cut(s) 739
BsaJI CCNNGG 3 cut(s) 205, 385, 469
Bse1I ACTGG 1 cut(s) 266
Bse8I GATNNNNATC 1 cut(s) 739
BseBI CCWGG 2 cut(s) 387, 471
BseDI CCNNGG 3 cut(s) 205, 385, 469
BseGI GGATG 1 cut(s) 580
BseJI GATNNNNATC 1 cut(s) 739
BseMII CTCAG 1 cut(s) 158
BseNI ACTGG 1 cut(s) 266
BseRI GAGGAG 1 cut(s) 743
Bsh1236I CGCG 1 cut(s) 62
BshFI GGCC 5 cut(s) 67, 210, 334, 507, 528
BshNI GGYRCC 1 cut(s) 694
BsiHKAI GWGCWC 2 cut(s) 11, 186
BsiHKCI CYCGRG 1 cut(s) 179
BslFI GGGAC 1 cut(s) 186
BsmFI GGGAC 1 cut(s) 186
BsnI GGCC 5 cut(s) 67, 210, 334, 507, 528
BsoBI CYCGRG 1 cut(s) 179
Bsp1286I GDGCHC 2 cut(s) 11, 186
Bsp143I GATC 2 cut(s) 175, 663
BspACI CCGC 2 cut(s) 261, 556
BspANI GGCC 5 cut(s) 67, 210, 334, 507, 528
BspCNI CTCAG 1 cut(s) 157
BspFNI CGCG 1 cut(s) 62
BspHI TCATGA 1 cut(s) 622
BspLI GGNNCC 2 cut(s) 446, 696
BspMAI CTGCAG 1 cut(s) 564
BspPI GGATC 1 cut(s) 671
BspT107I GGYRCC 1 cut(s) 694
BspTI CTTAAG 1 cut(s) 517
BsrBI CCGCTC 1 cut(s) 263
BsrI ACTGG 1 cut(s) 266
BssECI CCNNGG 3 cut(s) 205, 385, 469
BssMI GATC 2 cut(s) 175, 663
BssT1I CCWWGG 1 cut(s) 205
Bst2UI CCWGG 2 cut(s) 387, 471
Bst4CI ACNGT 2 cut(s) 140, 364
Bst6I CTCTTC 1 cut(s) 684
BstAFI CTTAAG 1 cut(s) 517
BstC8I GCNNGC 1 cut(s) 560
BstDEI CTNAG 2 cut(s) 144, 759
BstF5I GGATG 1 cut(s) 580
BstFNI CGCG 1 cut(s) 62
BstKTI GATC 2 cut(s) 178, 666
BstMBI GATC 2 cut(s) 175, 663
BstNI CCWGG 2 cut(s) 387, 471
BstNSI RCATGY 1 cut(s) 355
BstSCI CCNGG 2 cut(s) 385, 469
BstSFI CTRYAG 1 cut(s) 560
BstUI CGCG 1 cut(s) 62
BstV2I GAAGAC 1 cut(s) 123
BsuRI GGCC 5 cut(s) 67, 210, 334, 507, 528
BtsCI GGATG 1 cut(s) 580
BtsI GCAGTG 1 cut(s) 322
BtsIMutI CAGTG 1 cut(s) 322
Cac8I GCNNGC 1 cut(s) 560
CciI TCATGA 1 cut(s) 622
Cfr13I GGNCC 1 cut(s) 332
CseI GACGC 1 cut(s) 68
Csp6I GTAC 4 cut(s) 35, 295, 360, 457
CviAII CATG 3 cut(s) 352, 623, 685
CviQI GTAC 4 cut(s) 35, 295, 360, 457
DdeI CTNAG 2 cut(s) 144, 759
DpnI GATC 2 cut(s) 177, 665
DpnII GATC 2 cut(s) 175, 663
EaeI YGGCCR 1 cut(s) 505
Eam1104I CTCTTC 1 cut(s) 684
EarI CTCTTC 1 cut(s) 684
Ecl136II GAGCTC 2 cut(s) 9, 184
Eco130I CCWWGG 1 cut(s) 205
Eco147I AGGCCT 1 cut(s) 528
Eco24I GRGCYC 2 cut(s) 11, 186
Eco53kI GAGCTC 2 cut(s) 9, 184
Eco88I CYCGRG 1 cut(s) 179
EcoICRI GAGCTC 2 cut(s) 9, 184
EcoRII CCWGG 2 cut(s) 385, 469
EcoT14I CCWWGG 1 cut(s) 205
EcoT22I ATGCAT 1 cut(s) 357
EcoT38I GRGCYC 2 cut(s) 11, 186
ErhI CCWWGG 1 cut(s) 205
FaeI CATG 3 cut(s) 355, 626, 688
FaqI GGGAC 1 cut(s) 186
FatI CATG 3 cut(s) 351, 622, 684
FauI CCCGC 1 cut(s) 268
FauNDI CATATG 1 cut(s) 299
Fnu4HI GCNGC 1 cut(s) 556
FokI GGATG 1 cut(s) 587
FriOI GRGCYC 2 cut(s) 11, 186
Fsp4HI GCNGC 1 cut(s) 556
FspBI CTAG 1 cut(s) 426
GluI GCNGC 1 cut(s) 556
GsuI CTGGAG 2 cut(s) 249, 309
HaeIII GGCC 5 cut(s) 67, 210, 334, 507, 528
HgaI GACGC 1 cut(s) 68
Hin1II CATG 3 cut(s) 355, 626, 688
HinfI GANTC 3 cut(s) 54, 320, 601
HphI GGTGA 2 cut(s) 3, 458
Hpy166II GTNNAC 2 cut(s) 349, 457
Hpy188I TCNGA 4 cut(s) 46, 160, 175, 248
Hpy188III TCNNGA 9 cut(s) 76, 179, 222, 326, 400, 605, 623, 635, 744
Hpy8I GTNNAC 2 cut(s) 349, 457
Hpy99I CGWCG 2 cut(s) 62, 164
HpyAV CCTTC 4 cut(s) 165, 323, 430, 714
HpyCH4III ACNGT 2 cut(s) 140, 364
HpyCH4IV ACGT 1 cut(s) 755
HpyCH4V TGCA 8 cut(s) 190, 303, 315, 342, 355, 367, 562, 707
HpyF3I CTNAG 2 cut(s) 144, 759
HpySE526I ACGT 1 cut(s) 755
Hsp92II CATG 3 cut(s) 355, 626, 688
Kzo9I GATC 2 cut(s) 175, 663
LmnI GCTCC 3 cut(s) 6, 268, 450
LweI GCATC 1 cut(s) 749
MaeI CTAG 1 cut(s) 426
MaeII ACGT 1 cut(s) 755
MaeIII GTNAC 2 cut(s) 212, 317
MalI GATC 2 cut(s) 177, 665
MbiI CCGCTC 1 cut(s) 263
MboI GATC 2 cut(s) 175, 663
MboII GAAGA 6 cut(s) 116, 122, 125, 128, 428, 671
MfeI CAATTG 1 cut(s) 127
MhlI GDGCHC 2 cut(s) 11, 186
MluCI AATT 1 cut(s) 127
MlyI GAGTC 1 cut(s) 314
MmeI TCCRAC 2 cut(s) 183, 226
Mph1103I ATGCAT 1 cut(s) 357
MroXI GAANNNNTTC 1 cut(s) 420
MseI TTAA 2 cut(s) 518, 650
MslI CAYNNNNRTG 1 cut(s) 627
MspCI CTTAAG 1 cut(s) 517
MspR9I CCNGG 2 cut(s) 387, 471
MunI CAATTG 1 cut(s) 127
MvaI CCWGG 2 cut(s) 387, 471
MvnI CGCG 1 cut(s) 62
NdeI CATATG 1 cut(s) 299
NdeII GATC 2 cut(s) 175, 663
NlaIII CATG 3 cut(s) 355, 626, 688
NlaIV GGNNCC 2 cut(s) 446, 696
NmuCI GTSAC 2 cut(s) 212, 317
NsiI ATGCAT 1 cut(s) 357
NspI RCATGY 1 cut(s) 355
PaeR7I CTCGAG 1 cut(s) 179
PagI TCATGA 1 cut(s) 622
PceI AGGCCT 1 cut(s) 528
PdmI GAANNNNTTC 1 cut(s) 420
PfeI GAWTC 2 cut(s) 54, 601
PkrI GCNGC 1 cut(s) 557
PleI GAGTC 1 cut(s) 314
PpsI GAGTC 1 cut(s) 314
Psp124BI GAGCTC 2 cut(s) 11, 186
Psp6I CCWGG 2 cut(s) 385, 469
PspGI CCWGG 2 cut(s) 385, 469
PspN4I GGNNCC 2 cut(s) 446, 696
PspPI GGNCC 1 cut(s) 332
PstI CTGCAG 1 cut(s) 564
RsaI GTAC 4 cut(s) 36, 296, 361, 458
RsaNI GTAC 4 cut(s) 35, 295, 360, 457
RseI CAYNNNNRTG 1 cut(s) 627
SacI GAGCTC 2 cut(s) 11, 186
SaqAI TTAA 2 cut(s) 518, 650
SatI GCNGC 1 cut(s) 556
Sau3AI GATC 2 cut(s) 175, 663
Sau96I GGNCC 1 cut(s) 332
ScaI AGTACT 1 cut(s) 361
SchI GAGTC 1 cut(s) 314
ScrFI CCNGG 2 cut(s) 387, 471
SduI GDGCHC 2 cut(s) 11, 186
SetI ASST 8 cut(s) 11, 153, 186, 296, 407, 696, 725, 758
SfaNI GCATC 1 cut(s) 749
SfcI CTRYAG 1 cut(s) 560
Sfr274I CTCGAG 1 cut(s) 179
SlaI CTCGAG 1 cut(s) 179
SmiMI CAYNNNNRTG 1 cut(s) 627
SmlI CTYRAG 5 cut(s) 179, 400, 481, 517, 635
SmoI CTYRAG 5 cut(s) 179, 400, 481, 517, 635
Sse9I AATT 1 cut(s) 127
SseBI AGGCCT 1 cut(s) 528
SsiI CCGC 2 cut(s) 261, 556
SspMI CTAG 1 cut(s) 426
SstI GAGCTC 2 cut(s) 11, 186
StuI AGGCCT 1 cut(s) 528
StyD4I CCNGG 2 cut(s) 385, 469
StyI CCWWGG 1 cut(s) 205
TaaI ACNGT 2 cut(s) 140, 364
TaiI ACGT 1 cut(s) 758
TaqI TCGA 3 cut(s) 57, 180, 666
TasI AATT 1 cut(s) 127
TatI WGTACW 2 cut(s) 359, 456
TauI GCSGC 1 cut(s) 558
TfiI GAWTC 2 cut(s) 54, 601
Tru1I TTAA 2 cut(s) 518, 650
Tru9I TTAA 2 cut(s) 518, 650
TscAI CASTG 1 cut(s) 322
TseFI GTSAC 2 cut(s) 212, 317
Tsp45I GTSAC 2 cut(s) 212, 317
TspDTI ATGAA 4 cut(s) 123, 429, 451, 702
TspGWI ACGGA 1 cut(s) 227
TspRI CASTG 1 cut(s) 322
Vha464I CTTAAG 1 cut(s) 517
XceI RCATGY 1 cut(s) 355
XhoI CTCGAG 1 cut(s) 179
XmnI GAANNNNTTC 1 cut(s) 420
XspI CTAG 1 cut(s) 426
ZrmI AGTACT 1 cut(s) 361
Zsp2I ATGCAT 1 cut(s) 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.