Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0009899

Physical Location & Seq

Genomic Coordinates Forward (+)

11465735 .. 11469735

Gene Structure 4001 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1062200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1185 bp

ATGTCGGACGATGAGAGAGAGGAGAAGGAGCTGGATCTCACATCTCCAGAAGTCGTTACCAAATACAAGAGCGCCGCTGAAATTGTTAACAAGGCTTTGCAGCTGGTAATATCGGAATGCAAACCGAAGGCGAAGATTGTGGAACTTTGTGAGAAAGGAGATTCATACATCAGAGAGCAAACTGGTAACATGTACAAAAATGCGAAGAAGAAGATCGAACGAGGCGTTGCCTTCCCGACTTGCATTTCTGTGAACAACACTGTTTGCCATTTCTCTCCTCTTGCCAGTGATGAGTCAGTGTTGGAAGAAGGCGATATTCTGAAGATTGATTTGGGCTGTCATATTGATGGGTTCATTGCCATAGTTGCGCATACTCATGTTCTTCAAGCAGGGCCAGTCACAGGAAGGGCAGCTGATGTTATTGCAGCTGCTAATACTGCTGCTGAAGTTGCATTAAGGCTTGTTAGGCCAGGGAGAAAGAACAAAGATGTAACAGAAGCAATACAAAAGGTTGCAGCAGCTTACGACTGCAAAATTGTTGAAGGTGTTCTTAGCCATCAGCTCAAGCAGTTTGTGATAGACGGGAACAAGGTCATATTAAGTGCTTCTAATCCAGAGACAAGAGTTGACGAGGCAGAATTTGAGGAGAATGAAGTTTATGCAGTGGATATAGTTGCAAGCACAGGTGAAGGCAAGCCTAAGCTGCTGGATGAGAAGCAGACGACTATCTACAAGCGAGCTGTTGACAAGAGCTACCACTTGAAGATGAAAGCTTCTAGGTTTATTTTCAGCGAAATAAGCCAGAAATTTCCCATCATGCCATTCACTGCTAGAGCTTTGGAAGAGAAGAGGGCAAGGCTGGGCTTACTTGAATGCGTCAACCACGAGCTTTTGCAGCCATATCCTGTTCTGCATGAGAAGCCTGGTGATTTTGTTGCTCATATCAAGTTCACCATCTTGCTAATGCCAAATGGATCAGATCGGATCACATCTCATCCTGTACAGGAGCTGCAACCCACTAAACAAGTAGATGACCCCGAAATCAAAGCCTGGTTGTCTTTGGCCACAAAGAAGAAGAAAGGTGGTGGAAAGAAGAAGAAAGGTAAGAAGAGCGATAAGCCTGAGGAGTCTACGGAAGCTGAACCGATGGATGCAACGGCAAATGGTGCTGAATCTCAAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006355 GO:0007049 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009735 GO:0009755 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010941 GO:0016020 GO:0019219 GO:0019222 GO:0019899 GO:0022402 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031625 GO:0031974 GO:0031981 GO:0032870 GO:0032991 GO:0040008 GO:0042221 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044770 GO:0044843 GO:0045595 GO:0045597 GO:0045892 GO:0045934 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051302 GO:0051716 GO:0060255 GO:0060548 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1990904 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M24

Protein Analysis

395

Amino Acids

43.43

Weight (kDa)

6.41

Isoelectric Point (pI)

42.31

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M24	PF00557	21 - 222	1.9e-21	Metallopeptidase family M24

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009899)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51800 AT3G51800 AT3G51800
fragaria_vesca	FvH4_6g11120 FvH4_6g11120 FvH4_6g11120
malus_domestica	MD00G1062200.v1.1 MD12G1172200.v1.1
prunus_persica	Prupe.6G280500_v2.0.a1 Prupe.6G280500_v2.0.a1
pyrus_communis	pycom04g14310 pycom12g16340
rosa_chinensis	RchiOBHm_Chr3g0462561
rosa_laevigata	RLG00000024848
rosa_roxburghii	Rroxscaffold_6G00417390
rosa_rugosa	Rorug03G0057600 Rorug03G0057600
rosa_samantha	Rh3AG115600 Rh3CG122500 Rh3DG121200
rosa_wichuraiana	Rw3G009800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	369
AccI	GTMKAC	1 cut(s)	1130
AciI	CCGC	1 cut(s)	75
AclWI	GGATC	3 cut(s)	42, 982, 992
AcoI	YGGCCR	1 cut(s)	1062
AcsI	RAATTY	2 cut(s)	638, 806
AcuI	CTGAAG	2 cut(s)	341, 465
AfaI	GTAC	2 cut(s)	194, 1002
AfiI	CCNNNNNNNGG	1 cut(s)	401
AflIII	ACRYGT	1 cut(s)	189
AgsI	TTSAA	4 cut(s)	386, 542, 763, 872
AjnI	CCWGG	3 cut(s)	469, 922, 1049
AjuI	GAANNNNNNNTTGG	2 cut(s)	314, 346
Alw26I	GTCTC	1 cut(s)	611
AlwI	GGATC	3 cut(s)	42, 982, 992
AlwNI	CAGNNNCTG	1 cut(s)	1009
AoxI	GGCC	3 cut(s)	392, 467, 1062
ApoI	RAATTY	2 cut(s)	638, 806
Asp700I	GAANNNNTTC	1 cut(s)	546
AspLEI	GCGC	2 cut(s)	74, 370
AspS9I	GGNCC	1 cut(s)	392
AsuHPI	GGTGA	3 cut(s)	698, 938, 943
AxyI	CCTNAGG	1 cut(s)	1122
BalI	TGGCCA	1 cut(s)	1064
BauI	CACGAG	1 cut(s)	884
BccI	CCATC	5 cut(s)	341, 564, 821, 962, 1141
BceAI	ACGGC	1 cut(s)	1173
BciT130I	CCWGG	3 cut(s)	471, 924, 1051
BcoDI	GTCTC	1 cut(s)	611
BfaI	CTAG	2 cut(s)	777, 831
BfoI	RGCGCY	1 cut(s)	75
Bme1390I	CCNGG	3 cut(s)	471, 924, 1051
BmgT120I	GGNCC	1 cut(s)	392
BmrFI	CCNGG	3 cut(s)	471, 924, 1051
BmsI	GCATC	1 cut(s)	1141
BpmI	CTGGAG	1 cut(s)	30
Bpu10I	CCTNAGC	1 cut(s)	699
BpuEI	CTTGAG	2 cut(s)	548, 1161
BsaJI	CCNNGG	1 cut(s)	470
Bsc4I	CCNNNNNNNGG	1 cut(s)	401
Bse1I	ACTGG	3 cut(s)	187, 285, 395
Bse21I	CCTNAGG	1 cut(s)	1122
Bse3DI	GCAATG	1 cut(s)	354
BseBI	CCWGG	3 cut(s)	471, 924, 1051
BseDI	CCNNGG	1 cut(s)	470
BseGI	GGATG	3 cut(s)	715, 994, 1156
BseLI	CCNNNNNNNGG	1 cut(s)	401
BseMI	GCAATG	1 cut(s)	354
BseMII	CTCAG	1 cut(s)	1113
BseNI	ACTGG	3 cut(s)	187, 285, 395
BseRI	GAGGAG	4 cut(s)	35, 267, 659, 1139
BseYI	CCCAGC	1 cut(s)	859
BshFI	GGCC	3 cut(s)	394, 469, 1064
BslI	CCNNNNNNNGG	1 cut(s)	401
BsmAI	GTCTC	1 cut(s)	611
BsmI	GAATGC	2 cut(s)	122, 878
BsnI	GGCC	3 cut(s)	394, 469, 1064
Bsp1407I	TGTACA	2 cut(s)	192, 1000
Bsp143I	GATC	5 cut(s)	34, 213, 974, 979, 984
BspACI	CCGC	1 cut(s)	75
BspANI	GGCC	3 cut(s)	394, 469, 1064
BspCNI	CTCAG	1 cut(s)	1114
BspPI	GGATC	3 cut(s)	42, 982, 992
BspQI	GCTCTTC	1 cut(s)	1103
BsrDI	GCAATG	1 cut(s)	354
BsrGI	TGTACA	2 cut(s)	192, 1000
BsrI	ACTGG	3 cut(s)	187, 285, 395
BssECI	CCNNGG	1 cut(s)	470
BssMI	GATC	5 cut(s)	34, 213, 974, 979, 984
BssSI	CACGAG	1 cut(s)	884
Bst2BI	CACGAG	1 cut(s)	884
Bst2UI	CCWGG	3 cut(s)	471, 924, 1051
Bst4CI	ACNGT	1 cut(s)	262
Bst6I	CTCTTC	3 cut(s)	837, 842, 1103
BstAPI	GCANNNNNTGC	1 cut(s)	1166
BstAUI	TGTACA	2 cut(s)	192, 1000
BstC8I	GCNNGC	3 cut(s)	679, 695, 738
BstDEI	CTNAG	3 cut(s)	551, 699, 1122
BstF5I	GGATG	3 cut(s)	715, 994, 1156
BstH2I	RGCGCY	1 cut(s)	75
BstHHI	GCGC	2 cut(s)	74, 370
BstKTI	GATC	5 cut(s)	37, 216, 977, 982, 987
BstMAI	GTCTC	1 cut(s)	611
BstMBI	GATC	5 cut(s)	34, 213, 974, 979, 984
BstMWI	GCNNNNNNNGC	9 cut(s)	365, 437, 449, 466, 703, 798, 895, 919, 1166
BstNI	CCWGG	3 cut(s)	471, 924, 1051
BstNSI	RCATGY	1 cut(s)	193
BstSCI	CCNGG	3 cut(s)	469, 922, 1049
BstX2I	RGATCY	1 cut(s)	34
BstYI	RGATCY	1 cut(s)	34
Bsu36I	CCTNAGG	1 cut(s)	1122
BsuRI	GGCC	3 cut(s)	394, 469, 1064
BtsCI	GGATG	3 cut(s)	715, 994, 1156
BtsI	GCAGTG	2 cut(s)	669, 825
BtsIMutI	CAGTG	5 cut(s)	258, 292, 303, 669, 825
Cac8I	GCNNGC	3 cut(s)	679, 695, 738
CaiI	CAGNNNCTG	1 cut(s)	1009
CfoI	GCGC	2 cut(s)	74, 370
Cfr13I	GGNCC	1 cut(s)	392
CseI	GACGC	1 cut(s)	865
Csp6I	GTAC	2 cut(s)	193, 1001
CviAII	CATG	5 cut(s)	190, 377, 817, 914, 1182
CviQI	GTAC	2 cut(s)	193, 1001
DdeI	CTNAG	3 cut(s)	551, 699, 1122
DpnI	GATC	5 cut(s)	36, 215, 976, 981, 986
DpnII	GATC	5 cut(s)	34, 213, 974, 979, 984
EaeI	YGGCCR	1 cut(s)	1062
Eam1104I	CTCTTC	3 cut(s)	837, 842, 1103
EarI	CTCTTC	3 cut(s)	837, 842, 1103
Eco57I	CTGAAG	2 cut(s)	341, 465
Eco81I	CCTNAGG	1 cut(s)	1122
EcoRII	CCWGG	3 cut(s)	469, 922, 1049
FaeI	CATG	5 cut(s)	193, 380, 820, 917, 1185
FalI	AAGNNNNNCTT	2 cut(s)	534, 566
FatI	CATG	5 cut(s)	189, 376, 816, 913, 1181
FblI	GTMKAC	1 cut(s)	1130
FokI	GGATG	3 cut(s)	722, 981, 1163
FspBI	CTAG	2 cut(s)	777, 831
FspI	TGCGCA	1 cut(s)	369
GlaI	GCGC	2 cut(s)	73, 369
GsaI	CCCAGC	1 cut(s)	863
GsuI	CTGGAG	1 cut(s)	30
HaeII	RGCGCY	1 cut(s)	75
HaeIII	GGCC	3 cut(s)	394, 469, 1064
HgaI	GACGC	1 cut(s)	865
HhaI	GCGC	2 cut(s)	74, 370
Hin1II	CATG	5 cut(s)	193, 380, 820, 917, 1185
Hin6I	GCGC	2 cut(s)	72, 368
HinP1I	GCGC	2 cut(s)	72, 368
HincII	GTYRAC	4 cut(s)	88, 628, 745, 880
HindII	GTYRAC	4 cut(s)	88, 628, 745, 880
HindIII	AAGCTT	1 cut(s)	771
HinfI	GANTC	4 cut(s)	161, 293, 1127, 1172
HpaI	GTTAAC	1 cut(s)	88
HphI	GGTGA	3 cut(s)	698, 938, 943
Hpy166II	GTNNAC	7 cut(s)	88, 253, 628, 745, 880, 951, 1131
Hpy188I	TCNGA	6 cut(s)	7, 115, 173, 321, 979, 984
Hpy188III	TCNNGA	3 cut(s)	47, 235, 614
Hpy8I	GTNNAC	7 cut(s)	88, 253, 628, 745, 880, 951, 1131
HpyAV	CCTTC	7 cut(s)	19, 121, 241, 302, 399, 536, 683
HpyCH4III	ACNGT	1 cut(s)	262
HpyF10VI	GCNNNNNNNGC	9 cut(s)	365, 437, 449, 466, 703, 798, 895, 919, 1166
HpyF3I	CTNAG	3 cut(s)	551, 699, 1122
Hsp92II	CATG	5 cut(s)	193, 380, 820, 917, 1185
HspAI	GCGC	2 cut(s)	72, 368
KspAI	GTTAAC	1 cut(s)	88
Kzo9I	GATC	5 cut(s)	34, 213, 974, 979, 984
LguI	GCTCTTC	1 cut(s)	1103
LmnI	GCTCC	2 cut(s)	28, 1006
LweI	GCATC	1 cut(s)	1141
MaeI	CTAG	2 cut(s)	777, 831
MaeIII	GTNAC	4 cut(s)	55, 185, 397, 490
MalI	GATC	5 cut(s)	36, 215, 976, 981, 986
MboI	GATC	5 cut(s)	34, 213, 974, 979, 984
MflI	RGATCY	1 cut(s)	34
MlsI	TGGCCA	1 cut(s)	1064
MluCI	AATT	4 cut(s)	81, 534, 638, 806
MluNI	TGGCCA	1 cut(s)	1064
MlyI	GAGTC	2 cut(s)	302, 1136
MmeI	TCCRAC	1 cut(s)	282
MnlI	CCTC	7 cut(s)	13, 215, 288, 625, 637, 843, 1117
Mox20I	TGGCCA	1 cut(s)	1064
MroXI	GAANNNNTTC	1 cut(s)	546
MscI	TGGCCA	1 cut(s)	1064
MseI	TTAA	3 cut(s)	87, 455, 599
MslI	CAYNNNNRTG	3 cut(s)	248, 345, 375
Msp20I	TGGCCA	1 cut(s)	1064
MspA1I	CMGCKG	4 cut(s)	77, 103, 413, 428
MspR9I	CCNGG	3 cut(s)	471, 924, 1051
Mva1269I	GAATGC	2 cut(s)	122, 878
MvaI	CCWGG	3 cut(s)	471, 924, 1051
MwoI	GCNNNNNNNGC	9 cut(s)	365, 437, 449, 466, 703, 798, 895, 919, 1166
NdeII	GATC	5 cut(s)	34, 213, 974, 979, 984
NlaIII	CATG	5 cut(s)	193, 380, 820, 917, 1185
NmuCI	GTSAC	1 cut(s)	397
NsbI	TGCGCA	1 cut(s)	369
NspI	RCATGY	1 cut(s)	193
PciI	ACATGT	1 cut(s)	189
PciSI	GCTCTTC	1 cut(s)	1103
PcsI	WCGNNNNNNNCGW	1 cut(s)	222
PctI	GAATGC	2 cut(s)	122, 878
PdmI	GAANNNNTTC	1 cut(s)	546
PfeI	GAWTC	2 cut(s)	161, 1172
PleI	GAGTC	2 cut(s)	301, 1135
PpsI	GAGTC	2 cut(s)	301, 1135
PscI	ACATGT	1 cut(s)	189
Psp6I	CCWGG	3 cut(s)	469, 922, 1049
PspFI	CCCAGC	1 cut(s)	859
PspGI	CCWGG	3 cut(s)	469, 922, 1049
PspPI	GGNCC	1 cut(s)	392
PstNI	CAGNNNCTG	1 cut(s)	1009
PsuI	RGATCY	1 cut(s)	34
PvuII	CAGCTG	3 cut(s)	103, 413, 428
RsaI	GTAC	2 cut(s)	194, 1002
RsaNI	GTAC	2 cut(s)	193, 1001
RseI	CAYNNNNRTG	3 cut(s)	248, 345, 375
SapI	GCTCTTC	1 cut(s)	1103
SaqAI	TTAA	3 cut(s)	87, 455, 599
Sau3AI	GATC	5 cut(s)	34, 213, 974, 979, 984
Sau96I	GGNCC	1 cut(s)	392
SchI	GAGTC	2 cut(s)	302, 1136
ScrFI	CCNGG	3 cut(s)	471, 924, 1051
SfaNI	GCATC	1 cut(s)	1141
SmiMI	CAYNNNNRTG	3 cut(s)	248, 345, 375
SmlI	CTYRAG	2 cut(s)	563, 1176
SmoI	CTYRAG	2 cut(s)	563, 1176
Sse9I	AATT	4 cut(s)	81, 534, 638, 806
SsiI	CCGC	1 cut(s)	75
SspMI	CTAG	2 cut(s)	777, 831
StyD4I	CCNGG	3 cut(s)	469, 922, 1049
TaaI	ACNGT	1 cut(s)	262
TaqI	TCGA	1 cut(s)	216
TasI	AATT	4 cut(s)	81, 534, 638, 806
TatI	WGTACW	2 cut(s)	192, 1000
TauI	GCSGC	1 cut(s)	77
TfiI	GAWTC	2 cut(s)	161, 1172
Tru1I	TTAA	3 cut(s)	87, 455, 599
Tru9I	TTAA	3 cut(s)	87, 455, 599
TscAI	CASTG	5 cut(s)	265, 292, 303, 669, 832
TseFI	GTSAC	1 cut(s)	397
Tsp45I	GTSAC	1 cut(s)	397
TspDTI	ATGAA	4 cut(s)	153, 343, 666, 782
TspGWI	ACGGA	1 cut(s)	1148
TspRI	CASTG	5 cut(s)	265, 292, 303, 669, 832
XapI	RAATTY	2 cut(s)	638, 806
XceI	RCATGY	1 cut(s)	193
XmiI	GTMKAC	1 cut(s)	1130
XmnI	GAANNNNTTC	1 cut(s)	546
XspI	CTAG	2 cut(s)	777, 831

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.