Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr12

Orthogroup ID

OG0009899

Physical Location & Seq

Genomic Coordinates Forward (+)

25257079 .. 25261360

Gene Structure 4282 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD12G1172200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1188 bp

ATGTCGGACGATGAGAGAGAGGAGAAGGAGCTGGATCTCACATCTCCGGAAGTCGTCACCAAATACAAGAGCGCCGCTGAAATTGTTAACAAGGCTCTGCAGCTGGTAATATCCGAATGCAAACCGAAAGCGAAGATTGTGGACCTTTGTGAGAAAGGGGATTCATACATCAGAGAACAAACTGGCAACATGTACAAAAATGCGAAGAAGAAGATCGAACGAGGCGTTGCCTTCCCGACTTGCATTTCTGTGAACAACACTGTATGCCATTTCTCTCCTCTTGGCAGTGACGAGTCAGTGTTGGAAGAAGGCGATATTCTGAAGATTGATTTGGGCTGTCATATTGATGGGTTCATTGCCATAGTCGCACATACTCATGTTCTTCAAGCTGGACCGGTTACAGGAAGGGCTGCTGATGTTATTGCAGCTGCTAATACTGCTGCTGAAGTTGCTTTAAGGCTTGTTAGGCCAGGGAGAAAGAACAAAGATGTAACAGAGGCAATACAAAAGGTTGCAGCAGCTTACGACTGCAAAATTGTTGAAGGTGTTCTTAGTCATCAGCTCAAGCAGTTTGTGATAGACGGGAACAAGGTCATATTAAGTGTTTCTAATCCGGAGACAAGAGTTGACGATGCAGAATTTGAGGAGAATGAAGTTTATGCAGTTGATATAGTCGCAAGCACAGGTGAAGGCAAGCCTAAGCTGCTGGATGAGAAGCAGACGACAATCTATAAACGAGCTGTTGACAAGAGCTACCACTTGAAGATGAAAGCTTCTAGGTTTATTTTCAGTGAAATTAGCCAGAAATTTCCCATCATGCCATTCACTGCTAGAGCTTTGGAAGAGAAGAGGGCTAGGCTTGGTTTACTCGAATGCGTCAACCACGAGCTTTTGCAGCCATATCCTGTTTTGCACGAGAAGCCTGGTGACTTTGTTGCTCGTATCAAGTTCACCGTCTTGCTAATGCCAAATGGATCAGACCGGATCACATCTCATCCTGTACAGGAGCTGCAGCCCACTAAACAAGTAGATGATCCTGAAATCAAAGCCTGGTTGTCTTTGGCCACAAAGAAGAAGAAAGGTGGTGGAAAGAAGAAGAAAGGCAAGAAGAGCGATAAGCCCGAGGAGGAGTCTGCAGAAGCTGAACCGATGGACGCAACAACGAACGGTGCTGAGTCTCAAGCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0001558 GO:0003674 GO:0003676 GO:0003677 GO:0003700 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005730 GO:0005737 GO:0005886 GO:0006355 GO:0007049 GO:0007154 GO:0007165 GO:0008150 GO:0009719 GO:0009725 GO:0009733 GO:0009734 GO:0009735 GO:0009755 GO:0009889 GO:0009890 GO:0009892 GO:0009987 GO:0010033 GO:0010468 GO:0010556 GO:0010558 GO:0010605 GO:0010629 GO:0010941 GO:0016020 GO:0019219 GO:0019222 GO:0019899 GO:0022402 GO:0023052 GO:0031323 GO:0031324 GO:0031326 GO:0031327 GO:0031625 GO:0031974 GO:0031981 GO:0032870 GO:0032991 GO:0040008 GO:0042221 GO:0042981 GO:0043066 GO:0043067 GO:0043069 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044389 GO:0044422 GO:0044424 GO:0044428 GO:0044446 GO:0044464 GO:0044770 GO:0044843 GO:0045595 GO:0045597 GO:0045892 GO:0045934 GO:0048518 GO:0048519 GO:0048522 GO:0048523 GO:0050789 GO:0050793 GO:0050794 GO:0050896 GO:0051094 GO:0051128 GO:0051171 GO:0051172 GO:0051252 GO:0051253 GO:0051302 GO:0051716 GO:0060255 GO:0060548 GO:0065007 GO:0070013 GO:0070887 GO:0071310 GO:0071365 GO:0071495 GO:0071944 GO:0080090 GO:0097159 GO:0140110 GO:1901363 GO:1902679 GO:1903506 GO:1903507 GO:1990904 GO:2000112 GO:2000113 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Peptidase_M24

Protein Analysis

396

Amino Acids

43.55

Weight (kDa)

6.36

Isoelectric Point (pI)

40.42

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M24	PF00557	21 - 222	1.9e-21	Metallopeptidase family M24

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

Line Width

Align Labels (Cladogram)

Image

Tree File

Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready

iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0009899)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G51800 AT3G51800 AT3G51800
fragaria_vesca	FvH4_6g11120 FvH4_6g11120 FvH4_6g11120
malus_domestica	MD00G1062200.v1.1 MD12G1172200.v1.1
prunus_persica	Prupe.6G280500_v2.0.a1 Prupe.6G280500_v2.0.a1
pyrus_communis	pycom04g14310 pycom12g16340
rosa_chinensis	RchiOBHm_Chr3g0462561
rosa_laevigata	RLG00000024848
rosa_roxburghii	Rroxscaffold_6G00417390
rosa_rugosa	Rorug03G0057600 Rorug03G0057600
rosa_samantha	Rh3AG115600 Rh3CG122500 Rh3DG121200
rosa_wichuraiana	Rw3G009800

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccIII	TCCGGA	2 cut(s)	46, 613
AciI	CCGC	1 cut(s)	75
AclWI	GGATC	4 cut(s)	42, 982, 992, 1028
AcoI	YGGCCR	1 cut(s)	1062
AcsI	RAATTY	2 cut(s)	638, 806
AcuI	CTGAAG	2 cut(s)	341, 465
AfaI	GTAC	2 cut(s)	194, 1002
AfiI	CCNNNNNNNGG	1 cut(s)	401
AflIII	ACRYGT	1 cut(s)	189
AgeI	ACCGGT	1 cut(s)	394
AgsI	TTSAA	3 cut(s)	386, 542, 763
AjnI	CCWGG	3 cut(s)	469, 922, 1049
AjuI	GAANNNNNNNTTGG	2 cut(s)	314, 346
Alw26I	GTCTC	2 cut(s)	611, 1182
AlwI	GGATC	4 cut(s)	42, 982, 992, 1028
AlwNI	CAGNNNCTG	2 cut(s)	1009, 1142
Ama87I	CYCGRG	1 cut(s)	1121
Aor13HI	TCCGGA	2 cut(s)	46, 613
AoxI	GGCC	2 cut(s)	467, 1062
ApoI	RAATTY	2 cut(s)	638, 806
AsiGI	ACCGGT	1 cut(s)	394
Asp700I	GAANNNNTTC	1 cut(s)	546
AspLEI	GCGC	1 cut(s)	74
AspS9I	GGNCC	2 cut(s)	142, 392
AsuHPI	GGTGA	4 cut(s)	49, 698, 938, 943
AvaI	CYCGRG	1 cut(s)	1121
AvaII	GGWCC	2 cut(s)	142, 392
BalI	TGGCCA	1 cut(s)	1064
BauI	CACGAG	2 cut(s)	884, 914
BccI	CCATC	3 cut(s)	341, 821, 1144
BciT130I	CCWGG	3 cut(s)	471, 924, 1051
BcoDI	GTCTC	2 cut(s)	611, 1182
BfaI	CTAG	3 cut(s)	777, 831, 855
BfmI	CTRYAG	3 cut(s)	98, 1010, 1134
BfoI	RGCGCY	1 cut(s)	75
Bme1390I	CCNGG	3 cut(s)	471, 924, 1051
Bme18I	GGWCC	2 cut(s)	142, 392
BmeT110I	CYCGRG	1 cut(s)	1121
BmgT120I	GGNCC	2 cut(s)	142, 392
BmrFI	CCNGG	3 cut(s)	471, 924, 1051
BmsI	GCATC	1 cut(s)	622
Bpu10I	CCTNAGC	1 cut(s)	699
BpuEI	CTTGAG	2 cut(s)	548, 1164
BsaJI	CCNNGG	2 cut(s)	470, 1122
BsaWI	WCCGGW	4 cut(s)	46, 394, 613, 981
Bsc4I	CCNNNNNNNGG	1 cut(s)	401
Bse118I	RCCGGY	1 cut(s)	394
Bse1I	ACTGG	1 cut(s)	187
Bse3DI	GCAATG	1 cut(s)	354
BseAI	TCCGGA	2 cut(s)	46, 613
BseBI	CCWGG	3 cut(s)	471, 924, 1051
BseDI	CCNNGG	2 cut(s)	470, 1122
BseGI	GGATG	2 cut(s)	715, 994
BseLI	CCNNNNNNNGG	1 cut(s)	401
BseMI	GCAATG	1 cut(s)	354
BseMII	CTCAG	1 cut(s)	1164
BseNI	ACTGG	1 cut(s)	187
BseRI	GAGGAG	5 cut(s)	35, 267, 659, 1139, 1142
BshFI	GGCC	2 cut(s)	469, 1064
BshTI	ACCGGT	1 cut(s)	394
BsiHKCI	CYCGRG	1 cut(s)	1121
BsiSI	CCGG	4 cut(s)	47, 395, 614, 982
BslI	CCNNNNNNNGG	1 cut(s)	401
BsmAI	GTCTC	2 cut(s)	611, 1182
BsmI	GAATGC	2 cut(s)	122, 878
BsnI	GGCC	2 cut(s)	469, 1064
BsoBI	CYCGRG	1 cut(s)	1121
Bsp13I	TCCGGA	2 cut(s)	46, 613
Bsp1407I	TGTACA	2 cut(s)	192, 1000
Bsp143I	GATC	5 cut(s)	34, 213, 974, 984, 1033
BspACI	CCGC	1 cut(s)	75
BspANI	GGCC	2 cut(s)	469, 1064
BspCNI	CTCAG	1 cut(s)	1165
BspEI	TCCGGA	2 cut(s)	46, 613
BspMAI	CTGCAG	3 cut(s)	102, 1014, 1138
BspPI	GGATC	4 cut(s)	42, 982, 992, 1028
BspQI	GCTCTTC	1 cut(s)	1103
BsrDI	GCAATG	1 cut(s)	354
BsrFI	RCCGGY	1 cut(s)	394
BsrGI	TGTACA	2 cut(s)	192, 1000
BsrI	ACTGG	1 cut(s)	187
BssAI	RCCGGY	1 cut(s)	394
BssECI	CCNNGG	2 cut(s)	470, 1122
BssMI	GATC	5 cut(s)	34, 213, 974, 984, 1033
BssSI	CACGAG	2 cut(s)	884, 914
Bst2BI	CACGAG	2 cut(s)	884, 914
Bst2UI	CCWGG	3 cut(s)	471, 924, 1051
Bst4CI	ACNGT	3 cut(s)	262, 955, 1169
Bst6I	CTCTTC	3 cut(s)	837, 842, 1103
BstAUI	TGTACA	2 cut(s)	192, 1000
BstC8I	GCNNGC	2 cut(s)	679, 695
BstDEI	CTNAG	3 cut(s)	551, 699, 1173
BstF5I	GGATG	2 cut(s)	715, 994
BstH2I	RGCGCY	1 cut(s)	75
BstHHI	GCGC	1 cut(s)	74
BstKTI	GATC	5 cut(s)	37, 216, 977, 987, 1036
BstMAI	GTCTC	2 cut(s)	611, 1182
BstMBI	GATC	5 cut(s)	34, 213, 974, 984, 1033
BstMWI	GCNNNNNNNGC	8 cut(s)	365, 437, 449, 466, 703, 895, 919, 1110
BstNI	CCWGG	3 cut(s)	471, 924, 1051
BstNSI	RCATGY	1 cut(s)	193
BstSCI	CCNGG	3 cut(s)	469, 922, 1049
BstSFI	CTRYAG	3 cut(s)	98, 1010, 1134
BstX2I	RGATCY	1 cut(s)	34
BstYI	RGATCY	1 cut(s)	34
BsuRI	GGCC	2 cut(s)	469, 1064
BtsCI	GGATG	2 cut(s)	715, 994
BtsI	GCAGTG	2 cut(s)	292, 825
BtsIMutI	CAGTG	5 cut(s)	258, 292, 303, 796, 825
Cac8I	GCNNGC	2 cut(s)	679, 695
CaiI	CAGNNNCTG	2 cut(s)	1009, 1142
CfoI	GCGC	1 cut(s)	74
Cfr10I	RCCGGY	1 cut(s)	394
Cfr13I	GGNCC	2 cut(s)	142, 392
CseI	GACGC	2 cut(s)	865, 1163
Csp6I	GTAC	2 cut(s)	193, 1001
CspAI	ACCGGT	1 cut(s)	394
CviAII	CATG	4 cut(s)	190, 377, 817, 1185
CviQI	GTAC	2 cut(s)	193, 1001
DdeI	CTNAG	3 cut(s)	551, 699, 1173
DpnI	GATC	5 cut(s)	36, 215, 976, 986, 1035
DpnII	GATC	5 cut(s)	34, 213, 974, 984, 1033
EaeI	YGGCCR	1 cut(s)	1062
Eam1104I	CTCTTC	3 cut(s)	837, 842, 1103
EarI	CTCTTC	3 cut(s)	837, 842, 1103
Eco47I	GGWCC	2 cut(s)	142, 392
Eco57I	CTGAAG	2 cut(s)	341, 465
Eco88I	CYCGRG	1 cut(s)	1121
EcoRII	CCWGG	3 cut(s)	469, 922, 1049
FaeI	CATG	4 cut(s)	193, 380, 820, 1188
FalI	AAGNNNNNCTT	2 cut(s)	534, 566
FatI	CATG	4 cut(s)	189, 376, 816, 1184
FokI	GGATG	2 cut(s)	722, 981
FspBI	CTAG	3 cut(s)	777, 831, 855
GlaI	GCGC	1 cut(s)	73
HaeII	RGCGCY	1 cut(s)	75
HaeIII	GGCC	2 cut(s)	469, 1064
HapII	CCGG	4 cut(s)	47, 395, 614, 982
HgaI	GACGC	2 cut(s)	865, 1163
HhaI	GCGC	1 cut(s)	74
Hin1II	CATG	4 cut(s)	193, 380, 820, 1188
Hin6I	GCGC	1 cut(s)	72
HinP1I	GCGC	1 cut(s)	72
HincII	GTYRAC	4 cut(s)	88, 628, 745, 880
HindII	GTYRAC	4 cut(s)	88, 628, 745, 880
HindIII	AAGCTT	1 cut(s)	771
HinfI	GANTC	4 cut(s)	161, 293, 1130, 1175
HpaI	GTTAAC	1 cut(s)	88
HpaII	CCGG	4 cut(s)	47, 395, 614, 982
HphI	GGTGA	4 cut(s)	49, 698, 938, 943
Hpy166II	GTNNAC	8 cut(s)	88, 142, 253, 628, 745, 866, 880, 951
Hpy188I	TCNGA	5 cut(s)	7, 115, 173, 321, 979
Hpy188III	TCNNGA	4 cut(s)	47, 235, 614, 1037
Hpy8I	GTNNAC	8 cut(s)	88, 142, 253, 628, 745, 866, 880, 951
HpyAV	CCTTC	6 cut(s)	19, 241, 302, 399, 536, 683
HpyCH4III	ACNGT	3 cut(s)	262, 955, 1169
HpyF10VI	GCNNNNNNNGC	8 cut(s)	365, 437, 449, 466, 703, 895, 919, 1110
HpyF3I	CTNAG	3 cut(s)	551, 699, 1173
Hsp92II	CATG	4 cut(s)	193, 380, 820, 1188
HspAI	GCGC	1 cut(s)	72
Kpn2I	TCCGGA	2 cut(s)	46, 613
KspAI	GTTAAC	1 cut(s)	88
Kzo9I	GATC	5 cut(s)	34, 213, 974, 984, 1033
LguI	GCTCTTC	1 cut(s)	1103
LmnI	GCTCC	2 cut(s)	28, 1006
LweI	GCATC	1 cut(s)	622
MaeI	CTAG	3 cut(s)	777, 831, 855
MaeIII	GTNAC	5 cut(s)	55, 287, 397, 490, 926
MalI	GATC	5 cut(s)	36, 215, 976, 986, 1035
MboI	GATC	5 cut(s)	34, 213, 974, 984, 1033
MflI	RGATCY	1 cut(s)	34
MlsI	TGGCCA	1 cut(s)	1064
MluCI	AATT	5 cut(s)	81, 534, 638, 795, 806
MluNI	TGGCCA	1 cut(s)	1064
MlyI	GAGTC	3 cut(s)	302, 1139, 1184
MmeI	TCCRAC	1 cut(s)	282
MnlI	CCTC	8 cut(s)	13, 215, 288, 490, 637, 843, 1117, 1120
Mox20I	TGGCCA	1 cut(s)	1064
MroI	TCCGGA	2 cut(s)	46, 613
MroXI	GAANNNNTTC	1 cut(s)	546
MscI	TGGCCA	1 cut(s)	1064
MseI	TTAA	3 cut(s)	87, 455, 599
MslI	CAYNNNNRTG	3 cut(s)	248, 345, 375
Msp20I	TGGCCA	1 cut(s)	1064
MspA1I	CMGCKG	3 cut(s)	77, 103, 428
MspI	CCGG	4 cut(s)	47, 395, 614, 982
MspR9I	CCNGG	3 cut(s)	471, 924, 1051
Mva1269I	GAATGC	2 cut(s)	122, 878
MvaI	CCWGG	3 cut(s)	471, 924, 1051
MwoI	GCNNNNNNNGC	8 cut(s)	365, 437, 449, 466, 703, 895, 919, 1110
NdeII	GATC	5 cut(s)	34, 213, 974, 984, 1033
NlaIII	CATG	4 cut(s)	193, 380, 820, 1188
NmuCI	GTSAC	3 cut(s)	55, 287, 926
NspI	RCATGY	1 cut(s)	193
PciI	ACATGT	1 cut(s)	189
PciSI	GCTCTTC	1 cut(s)	1103
PcsI	WCGNNNNNNNCGW	1 cut(s)	222
PctI	GAATGC	2 cut(s)	122, 878
PdmI	GAANNNNTTC	1 cut(s)	546
PfeI	GAWTC	1 cut(s)	161
PinAI	ACCGGT	1 cut(s)	394
PleI	GAGTC	3 cut(s)	301, 1138, 1183
PpsI	GAGTC	3 cut(s)	301, 1138, 1183
PscI	ACATGT	1 cut(s)	189
Psp6I	CCWGG	3 cut(s)	469, 922, 1049
PspGI	CCWGG	3 cut(s)	469, 922, 1049
PspPI	GGNCC	2 cut(s)	142, 392
PstI	CTGCAG	3 cut(s)	102, 1014, 1138
PstNI	CAGNNNCTG	2 cut(s)	1009, 1142
PsuI	RGATCY	1 cut(s)	34
PvuII	CAGCTG	2 cut(s)	103, 428
RsaI	GTAC	2 cut(s)	194, 1002
RsaNI	GTAC	2 cut(s)	193, 1001
RseI	CAYNNNNRTG	3 cut(s)	248, 345, 375
SapI	GCTCTTC	1 cut(s)	1103
SaqAI	TTAA	3 cut(s)	87, 455, 599
Sau3AI	GATC	5 cut(s)	34, 213, 974, 984, 1033
Sau96I	GGNCC	2 cut(s)	142, 392
SchI	GAGTC	3 cut(s)	302, 1139, 1184
ScrFI	CCNGG	3 cut(s)	471, 924, 1051
SfaNI	GCATC	1 cut(s)	622
SfcI	CTRYAG	3 cut(s)	98, 1010, 1134
SinI	GGWCC	2 cut(s)	142, 392
SmiMI	CAYNNNNRTG	3 cut(s)	248, 345, 375
SmlI	CTYRAG	2 cut(s)	563, 1179
SmoI	CTYRAG	2 cut(s)	563, 1179
Sse9I	AATT	5 cut(s)	81, 534, 638, 795, 806
SsiI	CCGC	1 cut(s)	75
SspMI	CTAG	3 cut(s)	777, 831, 855
StyD4I	CCNGG	3 cut(s)	469, 922, 1049
TaaI	ACNGT	3 cut(s)	262, 955, 1169
TaqI	TCGA	2 cut(s)	216, 870
TasI	AATT	5 cut(s)	81, 534, 638, 795, 806
TatI	WGTACW	2 cut(s)	192, 1000
TauI	GCSGC	1 cut(s)	77
TfiI	GAWTC	1 cut(s)	161
Tru1I	TTAA	3 cut(s)	87, 455, 599
Tru9I	TTAA	3 cut(s)	87, 455, 599
TscAI	CASTG	5 cut(s)	265, 292, 303, 796, 832
TseFI	GTSAC	3 cut(s)	55, 287, 926
Tsp45I	GTSAC	3 cut(s)	55, 287, 926
TspDTI	ATGAA	4 cut(s)	153, 343, 666, 782
TspRI	CASTG	5 cut(s)	265, 292, 303, 796, 832
VpaK11BI	GGWCC	2 cut(s)	142, 392
XapI	RAATTY	2 cut(s)	638, 806
XceI	RCATGY	1 cut(s)	193
XmnI	GAANNNNTTC	1 cut(s)	546
XspI	CTAG	3 cut(s)	777, 831, 855

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.