Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0009115

Physical Location & Seq

Genomic Coordinates Forward (+)

14144390 .. 14148356

Gene Structure 3967 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1072100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGAGAAAGATTCATACTTTTCGCCTTTCTCATGCCCTTTTGTGCTTTGTTGTTGCTTTATTTTTGGCAATAATTGATGTTACTTCAGTAACTGAGAAGGAGATTTTACTTCAATTCAAAGGGAATGTAACAAATGATCCGTACAATCGTTTAGGCTCATGGGTTTTTACTGGGAATCCTTGCAAAGATTATGGTGGTGTGTTCTGTAACTCTGCTGGGTTTGTAGATAAGATAGTTTTGTTGAACACTAGCATTGGTGGAGTGTTGTCTCCGGCTTTGTCCGGGTTGAAATACCTGAGGGTTTTGACATTGTTTGGGAACAGATTTTCGGGAAGTATACCGAATGAGTATGCCGAGATTCGAACACTGTATAAGATCAATTTTAGTTCAAATGCGTTATCTGGGTCGATTCCAGAGTTTATAGGTGACTTGCCTAGCATTAGGCTTCTTGACTTGTCAAGGAATGGTTTTACTGGGGAGATTCCATCGGCTTTGTTTAAGTACTGTTACAAAACCAAGTTCGTTTCTTTGTCTCATAACGCTCTTTTGGGTTCAATGCCGGAATCGTTGGCGAATTGCTCGAATCTTGAAGGGTTTGATTTCTCTTTCAACAATCTCAGCGGTGGCATACCTTCGCGAATTTGCAATATTCCCAGGTTAAATTATTTGTCACTAAGAAGCAATGCGATGTCAGGGAGCGTTGTGCAGCAGCTTTCAGCTTGCCACAGCTTGAAACTACTGGATCTCAGTAGCAATTTGTTTACTGGTTCGGCACCATTTGGGGTTCTTGGATTGAGCAATCTTACTTATTTCAATGTATCGCATAATGAGTTTAACGGGAAGGTTCCTGATATTACAACTTGTAGTGAGAGAATGGAGTCTTTTGATGCTTCATGGAATGTGTTGGACGGAGAGGTTCCTCCAAGTATTACAAACTGTAGAAGTCTCAAGGTTTTGGAGTTGGGGTACAATGAGCTGGATGGGAGCATCCCAGATGTGCTCGGGAATCTGGTTAGGCTTGTCGTGATCCAATTGTGTAATAATTCAATAAGCGGAACTATTCCAGAAAAGCTTGCAAGCATTCAGCTGCTTCAGGTCTTGGATTTGCACAATCTCAACCTTGTTGGTGAAATACCCCATGATATAAGCAATTGCATGTTTCTGCGTGAGCTGGATGTTTCTGGTAACTCGTTGGAGGGAGAAATTCCTCAAGGTCTTTACAACATGACTAACCTTGAAATCCTTGACCTCCATAAAAACCAGCTCAATGGAAGCATCCCACCAGATGTAGGAAATCTGTCCAGCTTACAATATCTGGATCTTTCACAAAATTCACTTTTGGGGTCAATTCCTTCTTCCCTTGGAAACCTAGCAAAGTTGACTTATTTTAATCTCTCCTCCAATAAACTCTCAGGCACCATTCCTGCAGCTATTCAAGGTTACGGCTCCTATGCATTTATAAATAACCCCTTCCTCTGTGGTGCTCCATTGGACACGCCTTGCTCAGCAACTGGTAATGGTACTATCACTCCCACTTTGAGAAAACCCAAGGCTCTTCGTGTGCCTGCCATCTTTGCAATTGTTGCCGCTGCAGTGATTCTTAGTGGAGTATGTTTGGTATGCATTATGAACGTCAAAGCCCACAGAAAGAAGCAAGCCGATGTGACATTGGTTGTCGAGAGCACACCACTTGGCTCCACTGATTCAAATGTTATAATCGGGAAGCTGGTTCTCTTCAGCCAAAGTTTGCCCTCAAGGTATGAAGACTGGGAGTCCGGCACAAAAGCTTTGCTTGACAAAGAATGTATAATAGGTGGTGGATCAATTGGTACAGTCTACAGAACAAATTTTGAAGGTGGCATCTCAATTGCAGTAAAGAAGCTCGAGACTCTGGGAAGTATCAGAAATCAAGATGAATTTGAGCAAGAAATTGGACGACTGGGCAACCTTCAGCATCCCAATTTAGTAGCATTTCAAGGTTACTATTGGTCGTCGACAATGCAGTTGATGCTATCAGAATTTGTCCCGTATGGGAATTTGTATGACAATCTACACGGTCTTCATTATCCGGGACCAAGCACTAGCAGAGGCAATAGTGAACTGTATTGGTGTAGGCGATTTAAGATTGCTTTGGGAACGGCTAAGGCGCTTGCCTACCTTCACCATGACTGTAGACCTCCTATTCTCCATCTCAACATTAAATCCACCAACATACTACTAGATGAGAAATATGAGCCAAAGTTGTCTGATTATGGGTTAGGTAAATTGCTTCCCATGTTGGATAACTACGGTTTAACCAAATTCCACAATGTTGTTGGGTATGTTGCACCAGAGCTGGCTCAGAGTCTGAGACTAAGTGAGAAATGTGATGTCTATAGCTTTGGAGTGGTTCTTTTGGAACTGGTCACAGGGAGGAAACCGGTAGAGAGTCCAACTGCAAATGAGGTGGTGGTTTTGTGTGAATATGTTAGGGGATTATTGGAGACTGGCTTTGCATCAGATTGCTTTGACAGAAGCTTAAGGGATTTTGTAGAGAATGAACTGATCCAGATGATGAAATTAGGGTTGATTTGTACATCCGAGCTCCCTTCGAAAAGACCAAGCATGGCTGAGGTTATACAGGTTCTTGAATCCATCAGAAACGGATTGGAGGCTTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

886

Amino Acids

96.77

Weight (kDa)

6.03

Isoelectric Point (pI)

31.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRR_14	PF23598	306 - 419	1.1e-10	Leucine-rich repeat region
LRR_8	PF13855	388 - 446	2.6e-07	Leucine rich repeat
LRR_8	PF13855	410 - 470	1.5e-07	Leucine rich repeat
Pkinase	PF00069	601 - 874	5.1e-33	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	602 - 875	4.9e-33	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009115)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12460 AT1G12460
fragaria_vesca	FvH4_5g00970 FvH4_5g00970 FvH4_5g00970
malus_domestica	MD00G1072100.v1.1 MD04G1076300.v1.1
prunus_persica	Prupe.5G083400_v2.0.a1 Prupe.5G083400_v2.0.a1 Prupe.5G083400_v2.0.a1
pyrus_communis	pycom04g06740 pycom06g06590
rosa_chinensis	RchiOBHm_Chr7g0200411
rosa_laevigata	RLG00000003728
rosa_multiflora	Rmu_sc0000617.1_g000043
rosa_roxburghii	Rroxscaffold_3G00256020
rosa_rugosa	Rorug07G0060200
rosa_samantha	Rh7AG185400 Rh7BG188500 Rh7CG195700 Rh7DG189500
rosa_wichuraiana	Rw7G016250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1460, 1715
AccB1I	GGYRCC	2 cut(s)	772, 1415
AccI	GTMKAC	4 cut(s)	337, 1836, 1994, 2173
AccII	CGCG	1 cut(s)	637
AciI	CCGC	3 cut(s)	621, 1053, 1587
AclWI	GGATC	6 cut(s)	131, 750, 1021, 1326, 1828, 2539
AcsI	RAATTY	8 cut(s)	639, 1203, 1330, 1846, 1916, 2018, 2035, 2300
AcuI	CTGAAG	4 cut(s)	69, 1076, 1720, 1934
AfaI	GTAC	6 cut(s)	143, 503, 968, 1522, 1831, 2575
AfiI	CCNNNNNNNGG	1 cut(s)	1289
AflII	CTTAAG	1 cut(s)	2518
AgeI	ACCGGT	1 cut(s)	2419
AhdI	GACNNNNNGTC	1 cut(s)	1771
AjnI	CCWGG	1 cut(s)	653
Alw21I	GWGCWC	4 cut(s)	1002, 1486, 1685, 2586
Alw26I	GTCTC	6 cut(s)	273, 537, 950, 1880, 2344, 2477
AlwI	GGATC	6 cut(s)	131, 750, 1021, 1326, 1828, 2539
AlwNI	CAGNNNCTG	3 cut(s)	92, 1511, 2347
Ama87I	CYCGRG	2 cut(s)	1001, 1883
ApeKI	GCWGC	5 cut(s)	706, 709, 1087, 1427, 1589
ApoI	RAATTY	8 cut(s)	639, 1203, 1330, 1846, 1916, 2018, 2035, 2300
AsiGI	ACCGGT	1 cut(s)	2419
AspLEI	GCGC	1 cut(s)	2149
AspS9I	GGNCC	1 cut(s)	2072
AsuC2I	CCSGG	2 cut(s)	283, 2070
AsuHPI	GGTGA	3 cut(s)	437, 1139, 2153
AsuII	TTCGAA	2 cut(s)	361, 2591
AvaI	CYCGRG	2 cut(s)	1001, 1883
AvaII	GGWCC	1 cut(s)	2072
AxyI	CCTNAGG	1 cut(s)	296
BanI	GGYRCC	2 cut(s)	772, 1415
BanII	GRGCYC	1 cut(s)	2586
BbsI	GAAGAC	2 cut(s)	1770, 2051
Bbv12I	GWGCWC	4 cut(s)	1002, 1486, 1685, 2586
BbvCI	CCTCAGC	1 cut(s)	2610
BbvI	GCAGC	5 cut(s)	718, 721, 1074, 1439, 1576
BccI	CCATC	5 cut(s)	493, 974, 1577, 2196, 2642
BceAI	ACGGC	2 cut(s)	1459, 2154
BciT130I	CCWGG	1 cut(s)	655
BcnI	CCSGG	2 cut(s)	283, 2070
BcoDI	GTCTC	6 cut(s)	273, 537, 950, 1880, 2344, 2477
BfaI	CTAG	5 cut(s)	249, 435, 1370, 2082, 2219
BfmI	CTRYAG	6 cut(s)	937, 1425, 1590, 1837, 2170, 2374
BfoI	RGCGCY	1 cut(s)	2150
BfrI	CTTAAG	1 cut(s)	2518
BisI	GCNGC	6 cut(s)	707, 710, 1088, 1428, 1587, 1590
BlpI	GCTNAGC	1 cut(s)	1504
BlsI	GCNGC	6 cut(s)	708, 711, 1089, 1429, 1588, 1591
BmcAI	AGTACT	1 cut(s)	503
Bme1390I	CCNGG	3 cut(s)	283, 655, 2070
Bme18I	GGWCC	1 cut(s)	2072
BmeRI	GACNNNNNGTC	1 cut(s)	1771
BmeT110I	CYCGRG	2 cut(s)	1001, 1883
BmgT120I	GGNCC	1 cut(s)	2072
BmiI	GGNNCC	7 cut(s)	774, 846, 918, 1417, 1447, 1696, 2073
BmrFI	CCNGG	3 cut(s)	283, 655, 2070
BmrI	ACTGGG	4 cut(s)	180, 483, 1777, 1949
BmsI	GCATC	7 cut(s)	877, 996, 1284, 1869, 1963, 1998, 2504
BmuI	ACTGGG	4 cut(s)	180, 483, 1777, 1949
BpiI	GAAGAC	2 cut(s)	1770, 2051
Bpu10I	CCTNAGC	2 cut(s)	2142, 2610
Bpu1102I	GCTNAGC	1 cut(s)	1504
Bpu14I	TTCGAA	2 cut(s)	361, 2591
BpuEI	CTTGAG	3 cut(s)	932, 1194, 1738
BpuMI	CCSGG	2 cut(s)	283, 2070
BsaJI	CCNNGG	3 cut(s)	653, 1360, 1548
BsaWI	WCCGGW	1 cut(s)	2419
BsaXI	ACNNNNNCTCC	4 cut(s)	950, 980, 1513, 1543
Bsc4I	CCNNNNNNNGG	1 cut(s)	1289
Bse118I	RCCGGY	1 cut(s)	2419
Bse1I	ACTGG	9 cut(s)	175, 478, 744, 769, 1516, 1772, 1944, 2406, 2491
Bse21I	CCTNAGG	1 cut(s)	296
Bse3DI	GCAATG	1 cut(s)	688
BseBI	CCWGG	1 cut(s)	655
BseDI	CCNNGG	3 cut(s)	653, 1360, 1548
BseGI	GGATG	6 cut(s)	985, 987, 1180, 1275, 1954, 2576
BseLI	CCNNNNNNNGG	1 cut(s)	1289
BseMI	GCAATG	1 cut(s)	688
BseMII	CTCAG	9 cut(s)	84, 287, 631, 760, 1425, 1518, 2339, 2354, 2601
BseNI	ACTGG	9 cut(s)	175, 478, 744, 769, 1516, 1772, 1944, 2406, 2491
BseRI	GAGGAG	1 cut(s)	1387
BseXI	GCAGC	5 cut(s)	718, 721, 1074, 1439, 1576
BseYI	CCCAGC	1 cut(s)	215
BsgI	GTGCAG	1 cut(s)	725
Bsh1236I	CGCG	1 cut(s)	637
BshNI	GGYRCC	2 cut(s)	772, 1415
BshTI	ACCGGT	1 cut(s)	2419
BsiHKAI	GWGCWC	4 cut(s)	1002, 1486, 1685, 2586
BsiHKCI	CYCGRG	2 cut(s)	1001, 1883
BsiSI	CCGG	6 cut(s)	272, 282, 560, 1776, 2069, 2420
BslFI	GGGAC	2 cut(s)	2009, 2085
BslI	CCNNNNNNNGG	1 cut(s)	1289
BsmAI	GTCTC	6 cut(s)	273, 537, 950, 1880, 2344, 2477
BsmFI	GGGAC	2 cut(s)	2009, 2085
BsmI	GAATGC	1 cut(s)	1080
BsoBI	CYCGRG	2 cut(s)	1001, 1883
Bsp119I	TTCGAA	2 cut(s)	361, 2591
Bsp1286I	GDGCHC	4 cut(s)	1002, 1486, 1685, 2586
Bsp1407I	TGTACA	1 cut(s)	2573
Bsp143I	GATC	7 cut(s)	136, 375, 742, 1026, 1318, 1820, 2544
Bsp1720I	GCTNAGC	1 cut(s)	1504
Bsp68I	TCGCGA	1 cut(s)	637
BspACI	CCGC	3 cut(s)	621, 1053, 1587
BspCNI	CTCAG	9 cut(s)	85, 288, 630, 759, 1424, 1517, 2340, 2353, 2602
BspFNI	CGCG	1 cut(s)	637
BspLI	GGNNCC	7 cut(s)	774, 846, 918, 1417, 1447, 1696, 2073
BspMAI	CTGCAG	2 cut(s)	1429, 1594
BspPI	GGATC	6 cut(s)	131, 750, 1021, 1326, 1828, 2539
BspQI	GCTCTTC	1 cut(s)	1560
BspT104I	TTCGAA	2 cut(s)	361, 2591
BspT107I	GGYRCC	2 cut(s)	772, 1415
BspTI	CTTAAG	1 cut(s)	2518
BsrDI	GCAATG	1 cut(s)	688
BsrFI	RCCGGY	1 cut(s)	2419
BsrGI	TGTACA	1 cut(s)	2573
BsrI	ACTGG	9 cut(s)	175, 478, 744, 769, 1516, 1772, 1944, 2406, 2491
BssAI	RCCGGY	1 cut(s)	2419
BssECI	CCNNGG	3 cut(s)	653, 1360, 1548
BssMI	GATC	7 cut(s)	136, 375, 742, 1026, 1318, 1820, 2544
BssNAI	GTATAC	1 cut(s)	338
BssT1I	CCWWGG	2 cut(s)	1360, 1548
Bst1107I	GTATAC	1 cut(s)	338
Bst2UI	CCWGG	1 cut(s)	655
Bst4CI	ACNGT	8 cut(s)	369, 506, 938, 1834, 2057, 2103, 2171, 2291
Bst6I	CTCTTC	2 cut(s)	1560, 1739
BstAFI	CTTAAG	1 cut(s)	2518
BstAPI	GCANNNNNTGC	2 cut(s)	1583, 2008
BstAUI	TGTACA	1 cut(s)	2573
BstBI	TTCGAA	2 cut(s)	361, 2591
BstC8I	GCNNGC	7 cut(s)	721, 1074, 1078, 1566, 1656, 2151, 2337
BstF5I	GGATG	6 cut(s)	985, 987, 1180, 1275, 1954, 2576
BstFNI	CGCG	1 cut(s)	637
BstH2I	RGCGCY	1 cut(s)	2150
BstHHI	GCGC	1 cut(s)	2149
BstKTI	GATC	7 cut(s)	139, 378, 745, 1029, 1321, 1823, 2547
BstMAI	GTCTC	6 cut(s)	273, 537, 950, 1880, 2344, 2477
BstMBI	GATC	7 cut(s)	136, 375, 742, 1026, 1318, 1820, 2544
BstMWI	GCNNNNNNNGC	3 cut(s)	1574, 1583, 2008
BstNI	CCWGG	1 cut(s)	655
BstNSI	RCATGY	1 cut(s)	1159
BstSCI	CCNGG	3 cut(s)	281, 653, 2068
BstSFI	CTRYAG	6 cut(s)	937, 1425, 1590, 1837, 2170, 2374
BstUI	CGCG	1 cut(s)	637
BstV1I	GCAGC	5 cut(s)	718, 721, 1074, 1439, 1576
BstV2I	GAAGAC	2 cut(s)	1770, 2051
BstX2I	RGATCY	2 cut(s)	742, 1318
BstXI	CCANNNNNNTGG	1 cut(s)	1268
BstYI	RGATCY	2 cut(s)	742, 1318
BstZ17I	GTATAC	1 cut(s)	338
Bsu36I	CCTNAGG	1 cut(s)	296
BtgZI	GCGATG	1 cut(s)	701
BtsCI	GGATG	6 cut(s)	985, 987, 1180, 1275, 1954, 2576
BtsI	GCAGTG	1 cut(s)	1599
BtsIMutI	CAGTG	3 cut(s)	365, 1599, 1698
BtuMI	TCGCGA	1 cut(s)	637
Cac8I	GCNNGC	7 cut(s)	721, 1074, 1078, 1566, 1656, 2151, 2337
CaiI	CAGNNNCTG	3 cut(s)	92, 1511, 2347
CfoI	GCGC	1 cut(s)	2149
Cfr10I	RCCGGY	1 cut(s)	2419
Cfr13I	GGNCC	1 cut(s)	2072
Csp6I	GTAC	6 cut(s)	142, 502, 967, 1521, 1830, 2574
CspAI	ACCGGT	1 cut(s)	2419
CspCI	CAANNNNNGTGG	2 cut(s)	2427, 2462
CviAII	CATG	9 cut(s)	32, 159, 894, 1139, 1156, 1225, 2165, 2275, 2605
CviQI	GTAC	6 cut(s)	142, 502, 967, 1521, 1830, 2574
DpnI	GATC	7 cut(s)	138, 377, 744, 1028, 1320, 1822, 2546
DpnII	GATC	7 cut(s)	136, 375, 742, 1026, 1318, 1820, 2544
DriI	GACNNNNNGTC	1 cut(s)	1771
Eam1104I	CTCTTC	2 cut(s)	1560, 1739
Eam1105I	GACNNNNNGTC	1 cut(s)	1771
EarI	CTCTTC	2 cut(s)	1560, 1739
Ecl136II	GAGCTC	1 cut(s)	2584
Eco130I	CCWWGG	2 cut(s)	1360, 1548
Eco24I	GRGCYC	1 cut(s)	2586
Eco47I	GGWCC	1 cut(s)	2072
Eco53kI	GAGCTC	1 cut(s)	2584
Eco57I	CTGAAG	4 cut(s)	69, 1076, 1720, 1934
Eco81I	CCTNAGG	1 cut(s)	296
Eco88I	CYCGRG	2 cut(s)	1001, 1883
EcoICRI	GAGCTC	1 cut(s)	2584
EcoRII	CCWGG	1 cut(s)	653
EcoT14I	CCWWGG	2 cut(s)	1360, 1548
EcoT22I	ATGCAT	2 cut(s)	1456, 1625
EcoT38I	GRGCYC	1 cut(s)	2586
ErhI	CCWWGG	2 cut(s)	1360, 1548
FaeI	CATG	9 cut(s)	35, 162, 897, 1142, 1159, 1228, 2168, 2278, 2608
FalI	AAGNNNNNCTT	2 cut(s)	1776, 1808
FaqI	GGGAC	2 cut(s)	2009, 2085
FatI	CATG	9 cut(s)	31, 158, 893, 1138, 1155, 1224, 2164, 2274, 2604
FblI	GTMKAC	4 cut(s)	337, 1836, 1994, 2173
Fnu4HI	GCNGC	6 cut(s)	707, 710, 1088, 1428, 1587, 1590
FokI	GGATG	6 cut(s)	974, 992, 1187, 1262, 1941, 2563
FriOI	GRGCYC	1 cut(s)	2586
Fsp4HI	GCNGC	6 cut(s)	707, 710, 1088, 1428, 1587, 1590
FspBI	CTAG	5 cut(s)	249, 435, 1370, 2082, 2219
GlaI	GCGC	1 cut(s)	2148
GluI	GCNGC	6 cut(s)	707, 710, 1088, 1428, 1587, 1590
GsaI	CCCAGC	1 cut(s)	219
HaeII	RGCGCY	1 cut(s)	2150
HapII	CCGG	6 cut(s)	272, 282, 560, 1776, 2069, 2420
HhaI	GCGC	1 cut(s)	2149
Hin1II	CATG	9 cut(s)	35, 162, 897, 1142, 1159, 1228, 2168, 2278, 2608
Hin6I	GCGC	1 cut(s)	2147
HinP1I	GCGC	1 cut(s)	2147
HincII	GTYRAC	2 cut(s)	1380, 1995
HindII	GTYRAC	2 cut(s)	1380, 1995
HindIII	AAGCTT	3 cut(s)	1070, 1785, 2515
HpaII	CCGG	6 cut(s)	272, 282, 560, 1776, 2069, 2420
HphI	GGTGA	3 cut(s)	437, 1139, 2153
Hpy166II	GTNNAC	7 cut(s)	338, 762, 1380, 1837, 1995, 2099, 2174
Hpy188I	TCNGA	8 cut(s)	1904, 2017, 2248, 2343, 2349, 2500, 2581, 2639
Hpy8I	GTNNAC	7 cut(s)	338, 762, 1380, 1837, 1995, 2099, 2174
Hpy99I	CGWCG	1 cut(s)	1996
HpyCH4III	ACNGT	8 cut(s)	369, 506, 938, 1834, 2057, 2103, 2171, 2291
HpyCH4IV	ACGT	1 cut(s)	1632
HpyF10VI	GCNNNNNNNGC	3 cut(s)	1574, 1583, 2008
HpySE526I	ACGT	1 cut(s)	1632
Hsp92II	CATG	9 cut(s)	35, 162, 897, 1142, 1159, 1228, 2168, 2278, 2608
HspAI	GCGC	1 cut(s)	2147
Kzo9I	GATC	7 cut(s)	136, 375, 742, 1026, 1318, 1820, 2544
LguI	GCTCTTC	1 cut(s)	1560
LmnI	GCTCC	6 cut(s)	696, 984, 1451, 1489, 1700, 2589
Lsp1109I	GCAGC	5 cut(s)	718, 721, 1074, 1439, 1576
LweI	GCATC	7 cut(s)	877, 996, 1284, 1869, 1963, 1998, 2504
MaeI	CTAG	5 cut(s)	249, 435, 1370, 2082, 2219
MaeII	ACGT	1 cut(s)	1632
MalI	GATC	7 cut(s)	138, 377, 744, 1028, 1320, 1822, 2546
MboI	GATC	7 cut(s)	136, 375, 742, 1026, 1318, 1820, 2544
MboII	GAAGA	5 cut(s)	1347, 1547, 1726, 1775, 2051
MfeI	CAATTG	5 cut(s)	1031, 1150, 1578, 1824, 1866
MflI	RGATCY	2 cut(s)	742, 1318
MhlI	GDGCHC	4 cut(s)	1002, 1486, 1685, 2586
MlyI	GAGTC	5 cut(s)	887, 1781, 1882, 2353, 2437
MmeI	TCCRAC	4 cut(s)	885, 1173, 2259, 2456
Mph1103I	ATGCAT	2 cut(s)	1456, 1625
MseI	TTAA	8 cut(s)	498, 659, 834, 1389, 2121, 2199, 2294, 2519
MspA1I	CMGCKG	3 cut(s)	621, 1087, 1589
MspCI	CTTAAG	1 cut(s)	2518
MspI	CCGG	6 cut(s)	272, 282, 560, 1776, 2069, 2420
MspR9I	CCNGG	3 cut(s)	283, 655, 2070
MunI	CAATTG	5 cut(s)	1031, 1150, 1578, 1824, 1866
Mva1269I	GAATGC	1 cut(s)	1080
MvaI	CCWGG	1 cut(s)	655
MvnI	CGCG	1 cut(s)	637
MwoI	GCNNNNNNNGC	3 cut(s)	1574, 1583, 2008
NciI	CCSGG	2 cut(s)	283, 2070
NdeII	GATC	7 cut(s)	136, 375, 742, 1026, 1318, 1820, 2544
NlaIII	CATG	9 cut(s)	35, 162, 897, 1142, 1159, 1228, 2168, 2278, 2608
NlaIV	GGNNCC	7 cut(s)	774, 846, 918, 1417, 1447, 1696, 2073
NmeAIII	GCCGAG	1 cut(s)	379
NmuCI	GTSAC	4 cut(s)	425, 669, 1663, 2404
NruI	TCGCGA	1 cut(s)	637
NsiI	ATGCAT	2 cut(s)	1456, 1625
NspI	RCATGY	1 cut(s)	1159
NspV	TTCGAA	2 cut(s)	361, 2591
PaeR7I	CTCGAG	1 cut(s)	1883
PciSI	GCTCTTC	1 cut(s)	1560
PctI	GAATGC	1 cut(s)	1080
PfoI	TCCNGGA	1 cut(s)	2068
PinAI	ACCGGT	1 cut(s)	2419
PkrI	GCNGC	6 cut(s)	708, 711, 1089, 1429, 1588, 1591
PleI	GAGTC	5 cut(s)	886, 1780, 1882, 2352, 2436
PpsI	GAGTC	5 cut(s)	886, 1780, 1882, 2352, 2436
PsiI	TTATAA	2 cut(s)	1460, 1715
Psp124BI	GAGCTC	1 cut(s)	2586
Psp6I	CCWGG	1 cut(s)	653
PspFI	CCCAGC	1 cut(s)	215
PspGI	CCWGG	1 cut(s)	653
PspN4I	GGNNCC	7 cut(s)	774, 846, 918, 1417, 1447, 1696, 2073
PspPI	GGNCC	1 cut(s)	2072
PstI	CTGCAG	2 cut(s)	1429, 1594
PstNI	CAGNNNCTG	3 cut(s)	92, 1511, 2347
PsuI	RGATCY	2 cut(s)	742, 1318
PvuII	CAGCTG	1 cut(s)	1087
RruI	TCGCGA	1 cut(s)	637
RsaI	GTAC	6 cut(s)	143, 503, 968, 1522, 1831, 2575
RsaNI	GTAC	6 cut(s)	142, 502, 967, 1521, 1830, 2574
SacI	GAGCTC	1 cut(s)	2586
SalI	GTCGAC	1 cut(s)	1993
SapI	GCTCTTC	1 cut(s)	1560
SaqAI	TTAA	8 cut(s)	498, 659, 834, 1389, 2121, 2199, 2294, 2519
SatI	GCNGC	6 cut(s)	707, 710, 1088, 1428, 1587, 1590
Sau3AI	GATC	7 cut(s)	136, 375, 742, 1026, 1318, 1820, 2544
Sau96I	GGNCC	1 cut(s)	2072
ScaI	AGTACT	1 cut(s)	503
SchI	GAGTC	5 cut(s)	887, 1781, 1882, 2353, 2437
ScrFI	CCNGG	3 cut(s)	283, 655, 2070
SduI	GDGCHC	4 cut(s)	1002, 1486, 1685, 2586
SfaNI	GCATC	7 cut(s)	877, 996, 1284, 1869, 1963, 1998, 2504
SfcI	CTRYAG	6 cut(s)	937, 1425, 1590, 1837, 2170, 2374
Sfr274I	CTCGAG	1 cut(s)	1883
SfuI	TTCGAA	2 cut(s)	361, 2591
SinI	GGWCC	1 cut(s)	2072
SlaI	CTCGAG	1 cut(s)	1883
SmlI	CTYRAG	5 cut(s)	947, 1209, 1753, 1883, 2518
SmoI	CTYRAG	5 cut(s)	947, 1209, 1753, 1883, 2518
SsiI	CCGC	3 cut(s)	621, 1053, 1587
SspI	AATATT	1 cut(s)	649
SspMI	CTAG	5 cut(s)	249, 435, 1370, 2082, 2219
SstI	GAGCTC	1 cut(s)	2586
StyD4I	CCNGG	3 cut(s)	281, 653, 2068
StyI	CCWWGG	2 cut(s)	1360, 1548
TaaI	ACNGT	8 cut(s)	369, 506, 938, 1834, 2057, 2103, 2171, 2291
TaiI	ACGT	1 cut(s)	1635
TaqI	TCGA	7 cut(s)	361, 407, 581, 1677, 1884, 1994, 2591
TatI	WGTACW	2 cut(s)	501, 2573
TauI	GCSGC	1 cut(s)	1589
Tru1I	TTAA	8 cut(s)	498, 659, 834, 1389, 2121, 2199, 2294, 2519
Tru9I	TTAA	8 cut(s)	498, 659, 834, 1389, 2121, 2199, 2294, 2519
TscAI	CASTG	3 cut(s)	372, 1599, 1705
TseFI	GTSAC	4 cut(s)	425, 669, 1663, 2404
TseI	GCWGC	5 cut(s)	706, 709, 1087, 1427, 1589
Tsp45I	GTSAC	4 cut(s)	425, 669, 1663, 2404
TspDTI	ATGAA	7 cut(s)	882, 1643, 1776, 1929, 2051, 2553, 2570
TspGWI	ACGGA	3 cut(s)	129, 924, 2658
TspRI	CASTG	3 cut(s)	372, 1599, 1705
Vha464I	CTTAAG	1 cut(s)	2518
VpaK11BI	GGWCC	1 cut(s)	2072
XapI	RAATTY	8 cut(s)	639, 1203, 1330, 1846, 1916, 2018, 2035, 2300
XceI	RCATGY	1 cut(s)	1159
XcmI	CCANNNNNNNNNTGG	1 cut(s)	2312
XhoI	CTCGAG	1 cut(s)	1883
XmiI	GTMKAC	4 cut(s)	337, 1836, 1994, 2173
XspI	CTAG	5 cut(s)	249, 435, 1370, 2082, 2219
ZrmI	AGTACT	1 cut(s)	503
Zsp2I	ATGCAT	2 cut(s)	1456, 1625

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.