Basic Information

Type: gene

Biological Identity

Species

rosa_roxburghii

Chromosome

GWHEROQ00000003

Orthogroup ID

OG0009115

Physical Location & Seq

Genomic Coordinates Forward (+)

50460800 .. 50464417

Gene Structure 3618 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

Rroxscaffold_3G00256020.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2658 bp

ATGAGAATGCTTCATACTTTTCGTCTCTCTCATGCTCTTTTTTGCTTTCTTTGTTTCTATATTGGAATCATTGCTGTTTCATCAGTAACTGAGAAAGAGATTCTGCTTCAATTTAAAGGAAATGTAACTAGTGATCCTTACAGTAAATTAGTCTCATGGGTTCTTAGTGGAAATCCTTGCCAGGATTTTAGGGGTGTGTCCTGTAACACCGATGGGTTTGTAGAAAGGATAGTTCTTTGGAATACTAGCATAGGTGGAGTGTTGTCTCCGGCTTTAGCCGGGTTGAAATCATTGAGGGTTTTGACATTGTTTGGGAACAGGTTTGTGGGAAATTTACCACAAGAGTATGCTGATATTCAAACATTGTGGAAGATTAATGTTAGCTCAAATGCATTATCTGGGTCTATCCCTGAGTTTATAGGTGATTTGCCTAACATTAGGCTTCTGGATTTGTCAAGGAATGGTTTTACTGGGGAGATTCCATCAGCTTTATTCAAGTACTGTAACAAAACAAAGTTCATTTCTTTGTCACATAACAGTCTTCTGGGTTCAATTCCGGAATCATTGGCTAATTGCTTGAATCTTGAAGGGTTTGACTTCTCTTCCAACAACCTCAGCGGTGGCATTCCTTTGCGAATTTGTGATATTCCAAGGTTAGATTATGTGTCAGTGAGAAGCAATGCGTTATCTGGGAGTGTTGTGCAGCAGTTTTCGGCTTGTAAGAGCTTGAAACTACTGGATCTCGGTAGCAATTTGTTTACTGGTGCAGCGCCATTTGGGATTGTTGGATTGAGCAATCTTTCATACTTCAATGTGTCTCACAATGAATTTAATGGGGAGATTCCTGAGATTACAACTTGTAGTGAGACAATGGAGTATTTCGATGCTTCGTGGAATGAGTTGGATGGAGAGGTTCCGTTGAGCATTGCAAACTGTAGAGGTCTCAAGGTTTTGGAGTTGGGGTTTAACAGACTGACTGGAAGTATCCCGGAGGTGCTTGGAGATCTGGAGAGGCTTTTGGTGATTCAGTTATCCAATAATTCACTAAGTGGGACGATTCCAAAGACATTGACAAGCATTCAGCTGCTTCAGGTTTTGGATTTGCAGAATCTCAACCTTGTTGGTGAAATTCCCAGTGATATAAGCAATTGCATGTTTCTTCGAGAGCTGGATGTCTCTGGTAATGCTTTACAAGGAGAGATACCTCAGAAGCTTTACAACATGACTTACCTTGAAATCCTTGACCTCCATAAAAACCAGCTCAATGGAAGCATCCCACCAGAGCTGGGGAACCTTTCGAGATTACAATCTCTGGATCTGTCACAAAATTTGCTTTCTGGGCTGATTCCTTCTTCTCTTGAAAATCTACCAAAGTTGACGTATTTTAACCTCTCCTCCAATGATCTGTCAGGCACTATCCCCACAGCAGTCCAAGGTTACGGCCCCTTTGCTTTCATTAACAATCCGTATCTGTGTGGTGCCCCTCTGGACCAGCCTTGCTCAGCAAATGGTAATGGTACTGGGCCTTCTACTTCAAAGAAACCCAAGGCTCTTCGGTTGTCTGCCATTATTGCAATTGCTGCTGCTGCACTGATTCTTACTGGGGTTTGTCTAGTATTCATAATGAACATCATTGCCCGCAGAAAGAAGTTAGATGAGGGGACAATGGTTGTTGAGAGCACGCCACTTGGTTCAACTGAATCAAATGTCATTATTGGGAAGTTGGTTCTTTTCAGCAAAAGTTTGCCCTCAAGGTATGAAGACTGGGAATCAGGCACAAAAGCTTTACTTGACAAAGAATGCATAATAGGTGGTGGATCCCTTGGTACTGTCTACAGAACTACTTTCGAAGGTGGCATCTCAATTGCAGTGAAGAAGCTTGAGACTCTGGGAAGGATCAGAAACCAAGAGGAATTTGAGCAAGAAATTGGAAGACTGGGGAACCTTCGACATTCTAATTTAGTAGCATTTCAAGGGTACTACTGGTCCTCAACAATGCAGTTGATGCTATCCGAGTTTGTCCCAAATGGAAATTTGTATGACAATCTACATGGTCTTCATTACCCCGGACCAAGCACTAGCAGTGGAAATAGTGAACTCTATTGGTCTCGGAGATTTAAGATTGCTCTTGGAACAGCAAAAGCTCTTGCCTACCTTCACCATGACTGTAGACCTCCTCTTCTCCATCTCAACATTAAATCCACTAACATACTCTTAGATGAGAACTATGTGGCCAAGTTGTCTGATTATGGGCTGGGAAAATTGCTTCCTATTTTGGATAATCATGGTTTAACCAAATACCACAATGCCGTAGGGTATGTTGCGCCAGAGTTGGCTCAGAGTCTAAGACTGAGTGAGAAATGTGATGTGTATAGTTTTGGAGTAATTCTTTTGGAGCTGGTGACAGGAAGAAAACCAGTAGAGAGTCCAACAGCAAATCAGGTTGTGGTTTTGTGTGAATATGTTAGGGGTTTATTGGAGGGTGGCTTTGCTTCAGATTGTTTTGACAGAAGCTTAAGGGGTTTTGTAGAGAATGAACTGATCCAGGTTATGAAGTTGGGGTTGATTTGTACATCTGAGCTCCCTTCAAGAAGACCAAGCATGGCTGAGGTTGTTCAGGTTCTCGAGTCCATTAGAAATGGGTCGGAATCATCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

ko04010 ko04064 ko04620 ko04621 ko04624 ko04722 ko05133 ko05140 ko05142 ko05145 ko05152 ko05162 ko05164 map04010 map04064 map04620 map04621 map04624 map04722 map05133 map05140 map05142 map05145 map05152 map05162 map05164

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_4 LRR_6 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

885

Amino Acids

96.69

Weight (kDa)

5.76

Isoelectric Point (pI)

35.82

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
LRRNT_2	PF08263	31 - 69	1.5e-06	Leucine rich repeat N-terminal domain
LRR_8	PF13855	217 - 276	4e-07	Leucine rich repeat
LRR_14	PF23598	307 - 468	4.6e-10	Leucine-rich repeat region
LRR_8	PF13855	313 - 370	2.1e-06	Leucine rich repeat
LRR_8	PF13855	431 - 469	2.6e-07	Leucine rich repeat
Pkinase	PF00069	600 - 872	4.6e-32	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	601 - 874	8.9e-34	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0009115)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G12460 AT1G12460
fragaria_vesca	FvH4_5g00970 FvH4_5g00970 FvH4_5g00970
malus_domestica	MD00G1072100.v1.1 MD04G1076300.v1.1
prunus_persica	Prupe.5G083400_v2.0.a1 Prupe.5G083400_v2.0.a1 Prupe.5G083400_v2.0.a1
pyrus_communis	pycom04g06740 pycom06g06590
rosa_chinensis	RchiOBHm_Chr7g0200411
rosa_laevigata	RLG00000003728
rosa_multiflora	Rmu_sc0000617.1_g000043
rosa_roxburghii	Rroxscaffold_3G00256020
rosa_rugosa	Rorug07G0060200
rosa_samantha	Rh7AG185400 Rh7BG188500 Rh7CG195700 Rh7DG189500
rosa_wichuraiana	Rw7G016250

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1478
AccI	GTMKAC	2 cut(s)	1833, 2170
AccIII	TCCGGA	1 cut(s)	556
AciI	CCGC	2 cut(s)	618, 1639
AclWI	GGATC	7 cut(s)	128, 747, 1323, 1812, 1825, 1904, 2536
AcoI	YGGCCR	1 cut(s)	2232
AcsI	RAATTY	7 cut(s)	331, 636, 827, 1128, 1327, 1913, 2032
AcuI	CTGAAG	2 cut(s)	1073, 2478
AdeI	CACNNNGTG	1 cut(s)	1049
AfaI	GTAC	5 cut(s)	500, 1519, 1828, 1979, 2572
AfiI	CCNNNNNNNGG	1 cut(s)	1286
AflII	CTTAAG	1 cut(s)	2515
AhlI	ACTAGT	1 cut(s)	128
AjnI	CCWGG	2 cut(s)	180, 2544
Alw21I	GWGCWC	2 cut(s)	1682, 2583
Alw26I	GTCTC	9 cut(s)	29, 157, 270, 822, 860, 947, 1180, 1877, 2112
AlwI	GGATC	7 cut(s)	128, 747, 1323, 1812, 1825, 1904, 2536
AlwNI	CAGNNNCTG	1 cut(s)	89
Ama87I	CYCGRG	1 cut(s)	2624
Aor13HI	TCCGGA	1 cut(s)	556
AoxI	GGCC	3 cut(s)	1441, 1523, 2232
ApeKI	GCWGC	6 cut(s)	703, 767, 1084, 1580, 1583, 1586
ApoI	RAATTY	7 cut(s)	331, 636, 827, 1128, 1327, 1913, 2032
AseI	ATTAAT	1 cut(s)	375
AspLEI	GCGC	2 cut(s)	772, 2326
AspS9I	GGNCC	5 cut(s)	1442, 1489, 1523, 1986, 2069
AsuC2I	CCSGG	3 cut(s)	280, 989, 2067
AsuHPI	GGTGA	5 cut(s)	434, 1033, 1136, 2150, 2413
AsuII	TTCGAA	1 cut(s)	1848
AvaI	CYCGRG	1 cut(s)	2624
AvaII	GGWCC	3 cut(s)	1489, 1986, 2069
BaeGI	GKGCMC	1 cut(s)	1483
BalI	TGGCCA	1 cut(s)	2234
BamHI	GGATCC	1 cut(s)	1817
BanI	GGYRCC	1 cut(s)	1478
BanII	GRGCYC	1 cut(s)	2583
BarI	GAAGNNNNNNTAC	2 cut(s)	1510, 1542
BbsI	GAAGAC	5 cut(s)	533, 1767, 1939, 2048, 2599
Bbv12I	GWGCWC	2 cut(s)	1682, 2583
BbvCI	CCTCAGC	2 cut(s)	614, 2607
BbvI	GCAGC	6 cut(s)	715, 779, 1071, 1567, 1570, 1573
BccI	CCATC	4 cut(s)	206, 490, 899, 2193
BceAI	ACGGC	2 cut(s)	1456, 2294
BciT130I	CCWGG	2 cut(s)	182, 2546
BciVI	GTATCC	1 cut(s)	995
BcnI	CCSGG	3 cut(s)	280, 989, 2067
BcoDI	GTCTC	9 cut(s)	29, 157, 270, 822, 860, 947, 1180, 1877, 2112
BcuI	ACTAGT	1 cut(s)	128
BfaI	CTAG	4 cut(s)	129, 246, 1613, 2079
BfmI	CTRYAG	3 cut(s)	934, 1834, 2167
BfoI	RGCGCY	1 cut(s)	773
BfrI	CTTAAG	1 cut(s)	2515
BfuI	GTATCC	1 cut(s)	995
BglII	AGATCT	1 cut(s)	1003
BisI	GCNGC	6 cut(s)	704, 768, 1085, 1581, 1584, 1587
BlpI	GCTNAGC	1 cut(s)	1501
BlsI	GCNGC	6 cut(s)	705, 769, 1086, 1582, 1585, 1588
BmcAI	AGTACT	1 cut(s)	500
Bme1390I	CCNGG	5 cut(s)	182, 280, 989, 2067, 2546
Bme18I	GGWCC	3 cut(s)	1489, 1986, 2069
BmeT110I	CYCGRG	1 cut(s)	2624
BmgT120I	GGNCC	5 cut(s)	1442, 1489, 1523, 1986, 2069
BmiI	GGNNCC	6 cut(s)	915, 1292, 1444, 1480, 1819, 1943
BmrFI	CCNGG	5 cut(s)	182, 280, 989, 2067, 2546
BmrI	ACTGGG	6 cut(s)	480, 1128, 1530, 1611, 1774, 1946
BmsI	GCATC	4 cut(s)	874, 1281, 1866, 1995
BmuI	ACTGGG	6 cut(s)	480, 1128, 1530, 1611, 1774, 1946
BpiI	GAAGAC	5 cut(s)	533, 1767, 1939, 2048, 2599
BpmI	CTGGAG	1 cut(s)	1028
Bpu10I	CCTNAGC	2 cut(s)	614, 2607
Bpu1102I	GCTNAGC	1 cut(s)	1501
Bpu14I	TTCGAA	1 cut(s)	1848
BpuEI	CTTGAG	3 cut(s)	929, 1735, 1901
BpuMI	CCSGG	3 cut(s)	280, 989, 2067
BsaBI	GATNNNNATC	1 cut(s)	1306
BsaI	GGTCTC	2 cut(s)	947, 2112
BsaJI	CCNNGG	5 cut(s)	650, 1432, 1545, 1822, 2065
BsaWI	WCCGGW	1 cut(s)	556
BsaXI	ACNNNNNCTCC	4 cut(s)	947, 977, 1379, 1409
Bsc4I	CCNNNNNNNGG	1 cut(s)	1286
Bse3DI	GCAATG	4 cut(s)	69, 685, 924, 1632
Bse8I	GATNNNNATC	1 cut(s)	1306
BseAI	TCCGGA	1 cut(s)	556
BseBI	CCWGG	2 cut(s)	182, 2546
BseDI	CCNNGG	5 cut(s)	650, 1432, 1545, 1822, 2065
BseGI	GGATG	3 cut(s)	910, 1177, 1272
BseJI	GATNNNNATC	1 cut(s)	1306
BseLI	CCNNNNNNNGG	1 cut(s)	1286
BseMI	GCAATG	4 cut(s)	69, 685, 924, 1632
BseRI	GAGGAG	2 cut(s)	1384, 2166
BseSI	GKGCMC	1 cut(s)	1483
BseXI	GCAGC	6 cut(s)	715, 779, 1071, 1567, 1570, 1573
BseYI	CCCAGC	2 cut(s)	1285, 2254
BsgI	GTGCAG	3 cut(s)	722, 786, 1572
BshFI	GGCC	3 cut(s)	1443, 1525, 2234
BshNI	GGYRCC	1 cut(s)	1478
BsiHKAI	GWGCWC	2 cut(s)	1682, 2583
BsiHKCI	CYCGRG	1 cut(s)	2624
BsiSI	CCGG	5 cut(s)	269, 279, 557, 989, 2067
BslFI	GGGAC	3 cut(s)	1066, 1675, 2006
BslI	CCNNNNNNNGG	1 cut(s)	1286
BsmAI	GTCTC	9 cut(s)	29, 157, 270, 822, 860, 947, 1180, 1877, 2112
BsmBI	CGTCTC	1 cut(s)	29
BsmFI	GGGAC	3 cut(s)	1066, 1675, 2006
BsmI	GAATGC	4 cut(s)	12, 624, 1077, 1805
BsnI	GGCC	3 cut(s)	1443, 1525, 2234
Bso31I	GGTCTC	2 cut(s)	947, 2112
BsoBI	CYCGRG	1 cut(s)	2624
Bsp119I	TTCGAA	1 cut(s)	1848
Bsp1286I	GDGCHC	3 cut(s)	1483, 1682, 2583
Bsp13I	TCCGGA	1 cut(s)	556
Bsp1407I	TGTACA	1 cut(s)	2570
Bsp143I	GATC	8 cut(s)	133, 739, 1003, 1315, 1402, 1817, 1896, 2541
Bsp1720I	GCTNAGC	1 cut(s)	1501
BspACI	CCGC	2 cut(s)	618, 1639
BspANI	GGCC	3 cut(s)	1443, 1525, 2234
BspEI	TCCGGA	1 cut(s)	556
BspLI	GGNNCC	6 cut(s)	915, 1292, 1444, 1480, 1819, 1943
BspPI	GGATC	7 cut(s)	128, 747, 1323, 1812, 1825, 1904, 2536
BspQI	GCTCTTC	1 cut(s)	1557
BspT104I	TTCGAA	1 cut(s)	1848
BspT107I	GGYRCC	1 cut(s)	1478
BspTI	CTTAAG	1 cut(s)	2515
BspTNI	GGTCTC	2 cut(s)	947, 2112
BsrDI	GCAATG	4 cut(s)	69, 685, 924, 1632
BsrGI	TGTACA	1 cut(s)	2570
BssECI	CCNNGG	5 cut(s)	650, 1432, 1545, 1822, 2065
BssMI	GATC	8 cut(s)	133, 739, 1003, 1315, 1402, 1817, 1896, 2541
BssT1I	CCWWGG	4 cut(s)	650, 1432, 1545, 1822
Bst2UI	CCWGG	2 cut(s)	182, 2546
Bst4CI	ACNGT	6 cut(s)	143, 503, 539, 935, 1831, 2168
Bst6I	CTCTTC	3 cut(s)	607, 1557, 2184
BstAFI	CTTAAG	1 cut(s)	2515
BstAPI	GCANNNNNTGC	2 cut(s)	1580, 2005
BstAUI	TGTACA	1 cut(s)	2570
BstBI	TTCGAA	1 cut(s)	1848
BstC8I	GCNNGC	2 cut(s)	1639, 1682
BstF5I	GGATG	3 cut(s)	910, 1177, 1272
BstH2I	RGCGCY	1 cut(s)	773
BstHHI	GCGC	2 cut(s)	772, 2326
BstKTI	GATC	8 cut(s)	136, 742, 1006, 1318, 1405, 1820, 1899, 2544
BstMAI	GTCTC	9 cut(s)	29, 157, 270, 822, 860, 947, 1180, 1877, 2112
BstMBI	GATC	8 cut(s)	133, 739, 1003, 1315, 1402, 1817, 1896, 2541
BstMWI	GCNNNNNNNGC	5 cut(s)	1339, 1571, 1580, 1586, 2005
BstNI	CCWGG	2 cut(s)	182, 2546
BstNSI	RCATGY	1 cut(s)	1156
BstSCI	CCNGG	5 cut(s)	180, 278, 987, 2065, 2544
BstSFI	CTRYAG	3 cut(s)	934, 1834, 2167
BstSLI	GKGCMC	1 cut(s)	1483
BstV1I	GCAGC	6 cut(s)	715, 779, 1071, 1567, 1570, 1573
BstV2I	GAAGAC	5 cut(s)	533, 1767, 1939, 2048, 2599
BstX2I	RGATCY	4 cut(s)	739, 1003, 1315, 1817
BstXI	CCANNNNNNTGG	1 cut(s)	1265
BstYI	RGATCY	4 cut(s)	739, 1003, 1315, 1817
BsuI	GTATCC	1 cut(s)	995
BsuRI	GGCC	3 cut(s)	1443, 1525, 2234
BtsCI	GGATG	3 cut(s)	910, 1177, 1272
BtsI	GCAGTG	2 cut(s)	1875, 2089
BtsIMutI	CAGTG	5 cut(s)	675, 1141, 1589, 1875, 2089
Cac8I	GCNNGC	2 cut(s)	1639, 1682
CaiI	CAGNNNCTG	1 cut(s)	89
CfoI	GCGC	2 cut(s)	772, 2326
Cfr13I	GGNCC	5 cut(s)	1442, 1489, 1523, 1986, 2069
Csp6I	GTAC	5 cut(s)	499, 1518, 1827, 1978, 2571
CspCI	CAANNNNNGTGG	2 cut(s)	347, 382
CviAII	CATG	8 cut(s)	32, 156, 1153, 1222, 2051, 2162, 2285, 2602
CviQI	GTAC	5 cut(s)	499, 1518, 1827, 1978, 2571
DpnI	GATC	8 cut(s)	135, 741, 1005, 1317, 1404, 1819, 1898, 2543
DpnII	GATC	8 cut(s)	133, 739, 1003, 1315, 1402, 1817, 1896, 2541
DraI	TTTAAA	1 cut(s)	115
DraIII	CACNNNGTG	1 cut(s)	1049
EaeI	YGGCCR	1 cut(s)	2232
Eam1104I	CTCTTC	3 cut(s)	607, 1557, 2184
EarI	CTCTTC	3 cut(s)	607, 1557, 2184
Ecl136II	GAGCTC	1 cut(s)	2581
Eco130I	CCWWGG	4 cut(s)	650, 1432, 1545, 1822
Eco24I	GRGCYC	1 cut(s)	2583
Eco31I	GGTCTC	2 cut(s)	947, 2112
Eco47I	GGWCC	3 cut(s)	1489, 1986, 2069
Eco53kI	GAGCTC	1 cut(s)	2581
Eco57I	CTGAAG	2 cut(s)	1073, 2478
Eco88I	CYCGRG	1 cut(s)	2624
EcoICRI	GAGCTC	1 cut(s)	2581
EcoRII	CCWGG	2 cut(s)	180, 2544
EcoT14I	CCWWGG	4 cut(s)	650, 1432, 1545, 1822
EcoT22I	ATGCAT	2 cut(s)	394, 1805
EcoT38I	GRGCYC	1 cut(s)	2583
ErhI	CCWWGG	4 cut(s)	650, 1432, 1545, 1822
Esp3I	CGTCTC	1 cut(s)	29
FaeI	CATG	8 cut(s)	35, 159, 1156, 1225, 2054, 2165, 2288, 2605
FalI	AAGNNNNNCTT	2 cut(s)	1773, 1805
FaqI	GGGAC	3 cut(s)	1066, 1675, 2006
FatI	CATG	8 cut(s)	31, 155, 1152, 1221, 2050, 2161, 2284, 2601
FauI	CCCGC	1 cut(s)	1646
FblI	GTMKAC	2 cut(s)	1833, 2170
Fnu4HI	GCNGC	6 cut(s)	704, 768, 1085, 1581, 1584, 1587
FokI	GGATG	3 cut(s)	917, 1184, 1259
FriOI	GRGCYC	1 cut(s)	2583
Fsp4HI	GCNGC	6 cut(s)	704, 768, 1085, 1581, 1584, 1587
FspBI	CTAG	4 cut(s)	129, 246, 1613, 2079
GlaI	GCGC	2 cut(s)	771, 2325
GluI	GCNGC	6 cut(s)	704, 768, 1085, 1581, 1584, 1587
GsaI	CCCAGC	2 cut(s)	1289, 2258
GsuI	CTGGAG	1 cut(s)	1028
HaeII	RGCGCY	1 cut(s)	773
HaeIII	GGCC	3 cut(s)	1443, 1525, 2234
HapII	CCGG	5 cut(s)	269, 279, 557, 989, 2067
HhaI	GCGC	2 cut(s)	772, 2326
Hin1II	CATG	8 cut(s)	35, 159, 1156, 1225, 2054, 2165, 2288, 2605
Hin6I	GCGC	2 cut(s)	770, 2324
HinP1I	GCGC	2 cut(s)	770, 2324
HincII	GTYRAC	1 cut(s)	1377
HindII	GTYRAC	1 cut(s)	1377
HindIII	AAGCTT	4 cut(s)	1211, 1782, 1877, 2512
HpaII	CCGG	5 cut(s)	269, 279, 557, 989, 2067
HphI	GGTGA	5 cut(s)	434, 1033, 1136, 2150, 2413
Hpy166II	GTNNAC	5 cut(s)	759, 1377, 1834, 2096, 2171
Hpy188I	TCNGA	9 cut(s)	1209, 1901, 2014, 2112, 2245, 2340, 2497, 2578, 2647
Hpy8I	GTNNAC	5 cut(s)	759, 1377, 1834, 2096, 2171
HpyAV	CCTTC	8 cut(s)	581, 1359, 1536, 1844, 1887, 1955, 2165, 2595
HpyCH4III	ACNGT	6 cut(s)	143, 503, 539, 935, 1831, 2168
HpyCH4IV	ACGT	1 cut(s)	1379
HpyF10VI	GCNNNNNNNGC	5 cut(s)	1339, 1571, 1580, 1586, 2005
HpySE526I	ACGT	1 cut(s)	1379
Hsp92II	CATG	8 cut(s)	35, 159, 1156, 1225, 2054, 2165, 2288, 2605
HspAI	GCGC	2 cut(s)	770, 2324
Kpn2I	TCCGGA	1 cut(s)	556
Kzo9I	GATC	8 cut(s)	133, 739, 1003, 1315, 1402, 1817, 1896, 2541
LguI	GCTCTTC	1 cut(s)	1557
LmnI	GCTCC	2 cut(s)	2395, 2586
Lsp1109I	GCAGC	6 cut(s)	715, 779, 1071, 1567, 1570, 1573
LweI	GCATC	4 cut(s)	874, 1281, 1866, 1995
MaeI	CTAG	4 cut(s)	129, 246, 1613, 2079
MaeII	ACGT	1 cut(s)	1379
MaeIII	GTNAC	8 cut(s)	85, 124, 203, 503, 528, 1320, 1436, 2401
MalI	GATC	8 cut(s)	135, 741, 1005, 1317, 1404, 1819, 1898, 2543
MboI	GATC	8 cut(s)	133, 739, 1003, 1315, 1402, 1817, 1896, 2541
MfeI	CAATTG	3 cut(s)	1147, 1575, 1863
MflI	RGATCY	4 cut(s)	739, 1003, 1315, 1817
MhlI	GDGCHC	3 cut(s)	1483, 1682, 2583
MlsI	TGGCCA	1 cut(s)	2234
MluNI	TGGCCA	1 cut(s)	2234
MlyI	GAGTC	4 cut(s)	1879, 2350, 2434, 2636
MmeI	TCCRAC	5 cut(s)	630, 766, 882, 2453, 2625
Mox20I	TGGCCA	1 cut(s)	2234
Mph1103I	ATGCAT	2 cut(s)	394, 1805
MroI	TCCGGA	1 cut(s)	556
MscI	TGGCCA	1 cut(s)	2234
Msp20I	TGGCCA	1 cut(s)	2234
MspA1I	CMGCKG	2 cut(s)	618, 1084
MspCI	CTTAAG	1 cut(s)	2515
MspI	CCGG	5 cut(s)	269, 279, 557, 989, 2067
MspR9I	CCNGG	5 cut(s)	182, 280, 989, 2067, 2546
MunI	CAATTG	3 cut(s)	1147, 1575, 1863
Mva1269I	GAATGC	4 cut(s)	12, 624, 1077, 1805
MvaI	CCWGG	2 cut(s)	182, 2546
MwoI	GCNNNNNNNGC	5 cut(s)	1339, 1571, 1580, 1586, 2005
NciI	CCSGG	3 cut(s)	280, 989, 2067
NdeII	GATC	8 cut(s)	133, 739, 1003, 1315, 1402, 1817, 1896, 2541
NlaIII	CATG	8 cut(s)	35, 159, 1156, 1225, 2054, 2165, 2288, 2605
NlaIV	GGNNCC	6 cut(s)	915, 1292, 1444, 1480, 1819, 1943
NmuCI	GTSAC	3 cut(s)	528, 1320, 2401
NsiI	ATGCAT	2 cut(s)	394, 1805
NspI	RCATGY	1 cut(s)	1156
NspV	TTCGAA	1 cut(s)	1848
PaeR7I	CTCGAG	1 cut(s)	2624
PciSI	GCTCTTC	1 cut(s)	1557
PctI	GAATGC	4 cut(s)	12, 624, 1077, 1805
PfoI	TCCNGGA	1 cut(s)	987
PkrI	GCNGC	6 cut(s)	705, 769, 1086, 1582, 1585, 1588
PleI	GAGTC	4 cut(s)	1879, 2349, 2433, 2635
PpsI	GAGTC	4 cut(s)	1879, 2349, 2433, 2635
PshBI	ATTAAT	1 cut(s)	375
Psp124BI	GAGCTC	1 cut(s)	2583
Psp6I	CCWGG	2 cut(s)	180, 2544
PspFI	CCCAGC	2 cut(s)	1285, 2254
PspGI	CCWGG	2 cut(s)	180, 2544
PspN4I	GGNNCC	6 cut(s)	915, 1292, 1444, 1480, 1819, 1943
PspPI	GGNCC	5 cut(s)	1442, 1489, 1523, 1986, 2069
PstNI	CAGNNNCTG	1 cut(s)	89
PsuI	RGATCY	4 cut(s)	739, 1003, 1315, 1817
PvuII	CAGCTG	1 cut(s)	1084
RsaI	GTAC	5 cut(s)	500, 1519, 1828, 1979, 2572
RsaNI	GTAC	5 cut(s)	499, 1518, 1827, 1978, 2571
SacI	GAGCTC	1 cut(s)	2583
SapI	GCTCTTC	1 cut(s)	1557
SatI	GCNGC	6 cut(s)	704, 768, 1085, 1581, 1584, 1587
Sau3AI	GATC	8 cut(s)	133, 739, 1003, 1315, 1402, 1817, 1896, 2541
Sau96I	GGNCC	5 cut(s)	1442, 1489, 1523, 1986, 2069
ScaI	AGTACT	1 cut(s)	500
SchI	GAGTC	4 cut(s)	1879, 2350, 2434, 2636
ScrFI	CCNGG	5 cut(s)	182, 280, 989, 2067, 2546
SduI	GDGCHC	3 cut(s)	1483, 1682, 2583
SfaNI	GCATC	4 cut(s)	874, 1281, 1866, 1995
SfcI	CTRYAG	3 cut(s)	934, 1834, 2167
Sfr274I	CTCGAG	1 cut(s)	2624
SfuI	TTCGAA	1 cut(s)	1848
SinI	GGWCC	3 cut(s)	1489, 1986, 2069
SlaI	CTCGAG	1 cut(s)	2624
SmlI	CTYRAG	5 cut(s)	944, 1750, 1880, 2515, 2624
SmoI	CTYRAG	5 cut(s)	944, 1750, 1880, 2515, 2624
SpeI	ACTAGT	1 cut(s)	128
SsiI	CCGC	2 cut(s)	618, 1639
SspMI	CTAG	4 cut(s)	129, 246, 1613, 2079
SstI	GAGCTC	1 cut(s)	2583
StyD4I	CCNGG	5 cut(s)	180, 278, 987, 2065, 2544
StyI	CCWWGG	4 cut(s)	650, 1432, 1545, 1822
TaaI	ACNGT	6 cut(s)	143, 503, 539, 935, 1831, 2168
TaiI	ACGT	1 cut(s)	1382
TaqI	TCGA	6 cut(s)	882, 1162, 1298, 1848, 1948, 2625
TatI	WGTACW	2 cut(s)	498, 2570
TscAI	CASTG	5 cut(s)	675, 1141, 1596, 1875, 2089
TseFI	GTSAC	3 cut(s)	528, 1320, 2401
TseI	GCWGC	6 cut(s)	703, 767, 1084, 1580, 1583, 1586
Tsp45I	GTSAC	3 cut(s)	528, 1320, 2401
TspGWI	ACGGA	2 cut(s)	906, 1455
TspRI	CASTG	5 cut(s)	675, 1141, 1596, 1875, 2089
Vha464I	CTTAAG	1 cut(s)	2515
VpaK11BI	GGWCC	3 cut(s)	1489, 1986, 2069
VspI	ATTAAT	1 cut(s)	375
XapI	RAATTY	7 cut(s)	331, 636, 827, 1128, 1327, 1913, 2032
XceI	RCATGY	1 cut(s)	1156
XhoI	CTCGAG	1 cut(s)	2624
XmiI	GTMKAC	2 cut(s)	1833, 2170
XspI	CTAG	4 cut(s)	129, 246, 1613, 2079
ZrmI	AGTACT	1 cut(s)	500
Zsp2I	ATGCAT	2 cut(s)	394, 1805

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.