Rorug07G0060200

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0005530
Physical Location & Seq
Forward (+)
4386906 .. 4390205
3300 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0060200.1

Sequence Viewer

Length: 924 bp
ATGGATTCGGGTCTCAGTGATTGGGTCAAGTACTTGGAGGAGGAGTCATACCCAGTTCCCAGAGACTTCTTGGTCCTTCCCTTGTTTGTTCTCCTCTTCCCTACACTTAGGCTCTTCCTTGATAAATTCGTCTTTGAGAGATTAGCAAGATGGCTTATTTTTGGAAAGGGACAGAAGAAAGTTGAGACACATGATGAGCGGAAAAAAATTACTAAATTTAAAGAGTCGGCATGGAAATGCCTATACTTTTTTTCCGCAGAGGCTTTGGCCGTTTCCATAACATACAATGAGCCTTGGTTCTCAAAAACAAAAAACTTTTGGGTAGGGCCTGGCGATCAGGTTTGGCCCGACCAGAAAACGAAATTAAAATTGAAGGCACTCTACATGTATTCTGCTGGGTTTTACATGTACTCCAGCTTTGCTTTGGTGTTTTGGGAAACAAGGCGTTCCGACTTTTGGGTATCAATGGCTCATCATGTAGCAACAACAATATTGATTGTGTTCTCTTACACAGCGAGGTTTTCCCGAGTTGGTTCAATCATTTTAGCCCTCCATGAAGGATCTGATGTGTTTTTAGAAATTGCGAAACTGTCAAAATATTGTGGCTTAGAACTGATTGCCAGCGTTTCTTTTGTTATGTTTGTTCTTTCTTGGACAGTTCTACGTCTTATTTACTTTCCCTTCTGGATAATTTGGAGTACCTGCTATGAATCTCTTTTGAATTTGGACAAGGAAAAGCACATGGTGGATGGATCTATATACTATTACTTGTTTAATACTCTTCTAATTTGCTTGCTCATTCTTCATATTTATTGGTGGAAGTTGATGGTTTGGATGCTCATAAAGCAAATCCGCTCATGGGGGAAACTTGATGATGATGTTCGATCTGATTCCGAAGGTGATGATGATGAACACGATGATTGA

Protein Analysis

307

Amino Acids

36.44

Weight (kDa)

6.4

Isoelectric Point (pI)

33.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TRAM_LAG1_CLN8 PF03798 72 - 277 2.5e-48 TLC domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 71
Acc36I ACCTGC 1 cut(s) 710
AccBSI CCGCTC 2 cut(s) 199, 855
AciI CCGC 3 cut(s) 199, 255, 853
AclWI GGATC 2 cut(s) 568, 760
AcoI YGGCCR 1 cut(s) 267
AcsI RAATTY 3 cut(s) 125, 215, 721
AdeI CACNNNGTG 1 cut(s) 745
AfaI GTAC 3 cut(s) 32, 410, 700
AfiI CCNNNNNNNGG 2 cut(s) 456, 859
AflIII ACRYGT 2 cut(s) 384, 405
AgsI TTSAA 3 cut(s) 373, 537, 721
AjnI CCWGG 1 cut(s) 328
AluBI AGCT 1 cut(s) 417
AluI AGCT 1 cut(s) 417
Alw26I GTCTC 3 cut(s) 17, 57, 179
AlwI GGATC 2 cut(s) 568, 760
Ama87I CYCGRG 1 cut(s) 525
AoxI GGCC 3 cut(s) 267, 326, 344
ApoI RAATTY 3 cut(s) 125, 215, 721
AspS9I GGNCC 3 cut(s) 73, 326, 345
AsuHPI GGTGA 1 cut(s) 911
AvaI CYCGRG 1 cut(s) 525
AvaII GGWCC 1 cut(s) 73
BarI GAAGNNNNNNTAC 2 cut(s) 365, 397
BccI CCATC 3 cut(s) 144, 743, 820
BceAI ACGGC 1 cut(s) 254
BciT130I CCWGG 1 cut(s) 330
BcoDI GTCTC 3 cut(s) 17, 57, 179
BfuAI ACCTGC 1 cut(s) 710
BmcAI AGTACT 1 cut(s) 32
Bme1390I CCNGG 1 cut(s) 330
Bme18I GGWCC 1 cut(s) 73
BmeT110I CYCGRG 1 cut(s) 525
BmgT120I GGNCC 3 cut(s) 73, 326, 345
BmrFI CCNGG 1 cut(s) 330
BmrI ACTGGG 1 cut(s) 47
BmsI GCATC 1 cut(s) 825
BmuI ACTGGG 1 cut(s) 47
BpmI CTGGAG 1 cut(s) 397
BsaI GGTCTC 1 cut(s) 17
BsaJI CCNNGG 1 cut(s) 293
BsaXI ACNNNNNCTCC 4 cut(s) 29, 59, 395, 425
Bsc4I CCNNNNNNNGG 2 cut(s) 456, 859
Bse1I ACTGG 1 cut(s) 53
BseBI CCWGG 1 cut(s) 330
BseDI CCNNGG 1 cut(s) 293
BseGI GGATG 2 cut(s) 754, 840
BseLI CCNNNNNNNGG 2 cut(s) 456, 859
BseMII CTCAG 1 cut(s) 28
BseNI ACTGG 1 cut(s) 53
BseRI GAGGAG 3 cut(s) 53, 56, 83
BseYI CCCAGC 1 cut(s) 395
BshFI GGCC 3 cut(s) 269, 328, 346
BsiHKCI CYCGRG 1 cut(s) 525
BslFI GGGAC 1 cut(s) 183
BslI CCNNNNNNNGG 2 cut(s) 456, 859
BsmAI GTCTC 3 cut(s) 17, 57, 179
BsmFI GGGAC 1 cut(s) 183
BsnI GGCC 3 cut(s) 269, 328, 346
Bso31I GGTCTC 1 cut(s) 17
BsoBI CYCGRG 1 cut(s) 525
Bsp143I GATC 4 cut(s) 334, 560, 752, 884
BspACI CCGC 3 cut(s) 199, 255, 853
BspANI GGCC 3 cut(s) 269, 328, 346
BspCNI CTCAG 1 cut(s) 27
BspMI ACCTGC 1 cut(s) 710
BspPI GGATC 2 cut(s) 568, 760
BspQI GCTCTTC 1 cut(s) 119
BspTNI GGTCTC 1 cut(s) 17
BsrBI CCGCTC 2 cut(s) 199, 855
BsrI ACTGG 1 cut(s) 53
BssECI CCNNGG 1 cut(s) 293
BssMI GATC 4 cut(s) 334, 560, 752, 884
BssT1I CCWWGG 1 cut(s) 293
Bst2UI CCWGG 1 cut(s) 330
Bst4CI ACNGT 2 cut(s) 591, 658
Bst6I CTCTTC 3 cut(s) 101, 119, 786
BstC8I GCNNGC 2 cut(s) 622, 794
BstDEI CTNAG 3 cut(s) 14, 107, 607
BstF5I GGATG 2 cut(s) 754, 840
BstKTI GATC 4 cut(s) 337, 563, 755, 887
BstMAI GTCTC 3 cut(s) 17, 57, 179
BstMBI GATC 4 cut(s) 334, 560, 752, 884
BstMWI GCNNNNNNNGC 1 cut(s) 844
BstNI CCWGG 1 cut(s) 330
BstNSI RCATGY 2 cut(s) 388, 409
BstSCI CCNGG 1 cut(s) 328
BstX2I RGATCY 2 cut(s) 560, 752
BstYI RGATCY 2 cut(s) 560, 752
BsuRI GGCC 3 cut(s) 269, 328, 346
BtsCI GGATG 2 cut(s) 754, 840
BtsIMutI CAGTG 1 cut(s) 22
BveI ACCTGC 1 cut(s) 710
Cac8I GCNNGC 2 cut(s) 622, 794
Cfr13I GGNCC 3 cut(s) 73, 326, 345
Csp6I GTAC 3 cut(s) 31, 409, 699
CviAII CATG 8 cut(s) 191, 231, 385, 406, 476, 554, 742, 858
CviQI GTAC 3 cut(s) 31, 409, 699
DdeI CTNAG 3 cut(s) 14, 107, 607
DpnI GATC 4 cut(s) 336, 562, 754, 886
DpnII GATC 4 cut(s) 334, 560, 752, 884
DraI TTTAAA 1 cut(s) 220
DraIII CACNNNGTG 1 cut(s) 745
DrdI GACNNNNNNGTC 1 cut(s) 71
DseDI GACNNNNNNGTC 1 cut(s) 71
EaeI YGGCCR 1 cut(s) 267
Eam1104I CTCTTC 3 cut(s) 101, 119, 786
EarI CTCTTC 3 cut(s) 101, 119, 786
Eco130I CCWWGG 1 cut(s) 293
Eco31I GGTCTC 1 cut(s) 17
Eco47I GGWCC 1 cut(s) 73
Eco88I CYCGRG 1 cut(s) 525
EcoO109I RGGNCCY 1 cut(s) 326
EcoRII CCWGG 1 cut(s) 328
EcoT14I CCWWGG 1 cut(s) 293
ErhI CCWWGG 1 cut(s) 293
FaeI CATG 8 cut(s) 194, 234, 388, 409, 479, 557, 745, 861
FaqI GGGAC 1 cut(s) 183
FatI CATG 8 cut(s) 190, 230, 384, 405, 475, 553, 741, 857
FokI GGATG 2 cut(s) 761, 847
GsaI CCCAGC 1 cut(s) 399
GsuI CTGGAG 1 cut(s) 397
HaeIII GGCC 3 cut(s) 269, 328, 346
Hin1II CATG 8 cut(s) 194, 234, 388, 409, 479, 557, 745, 861
HinfI GANTC 5 cut(s) 5, 44, 224, 710, 890
HphI GGTGA 1 cut(s) 911
Hpy188I TCNGA 4 cut(s) 451, 565, 889, 895
Hpy188III TCNNGA 2 cut(s) 525, 685
HpyAV CCTTC 5 cut(s) 86, 367, 551, 691, 890
HpyCH4III ACNGT 2 cut(s) 591, 658
HpyCH4IV ACGT 1 cut(s) 664
HpyF10VI GCNNNNNNNGC 1 cut(s) 844
HpyF3I CTNAG 3 cut(s) 14, 107, 607
HpySE526I ACGT 1 cut(s) 664
Hsp92II CATG 8 cut(s) 194, 234, 388, 409, 479, 557, 745, 861
Kzo9I GATC 4 cut(s) 334, 560, 752, 884
LguI GCTCTTC 1 cut(s) 119
LweI GCATC 1 cut(s) 825
MaeII ACGT 1 cut(s) 664
MalI GATC 4 cut(s) 336, 562, 754, 886
MbiI CCGCTC 2 cut(s) 199, 855
MboI GATC 4 cut(s) 334, 560, 752, 884
MboII GAAGA 5 cut(s) 88, 106, 187, 773, 794
MflI RGATCY 2 cut(s) 560, 752
MluCI AATT 9 cut(s) 125, 207, 215, 362, 368, 579, 690, 721, 786
MlyI GAGTC 2 cut(s) 53, 233
MmeI TCCRAC 1 cut(s) 474
MnlI CCTC 6 cut(s) 31, 34, 104, 253, 510, 560
MseI TTAA 3 cut(s) 219, 365, 774
MslI CAYNNNNRTG 1 cut(s) 235
MspR9I CCNGG 1 cut(s) 330
MvaI CCWGG 1 cut(s) 330
MwoI GCNNNNNNNGC 1 cut(s) 844
NdeII GATC 4 cut(s) 334, 560, 752, 884
NlaIII CATG 8 cut(s) 194, 234, 388, 409, 479, 557, 745, 861
NspI RCATGY 2 cut(s) 388, 409
PciI ACATGT 2 cut(s) 384, 405
PciSI GCTCTTC 1 cut(s) 119
PfeI GAWTC 3 cut(s) 5, 710, 890
PleI GAGTC 2 cut(s) 52, 232
PpsI GAGTC 2 cut(s) 52, 232
PscI ACATGT 2 cut(s) 384, 405
Psp6I CCWGG 1 cut(s) 328
PspFI CCCAGC 1 cut(s) 395
PspGI CCWGG 1 cut(s) 328
PspPI GGNCC 3 cut(s) 73, 326, 345
PsuI RGATCY 2 cut(s) 560, 752
RsaI GTAC 3 cut(s) 32, 410, 700
RsaNI GTAC 3 cut(s) 31, 409, 699
RseI CAYNNNNRTG 1 cut(s) 235
SapI GCTCTTC 1 cut(s) 119
SaqAI TTAA 3 cut(s) 219, 365, 774
Sau3AI GATC 4 cut(s) 334, 560, 752, 884
Sau96I GGNCC 3 cut(s) 73, 326, 345
ScaI AGTACT 1 cut(s) 32
SchI GAGTC 2 cut(s) 53, 233
ScrFI CCNGG 1 cut(s) 330
SetI ASST 6 cut(s) 342, 419, 521, 667, 704, 901
SfaNI GCATC 1 cut(s) 825
SinI GGWCC 1 cut(s) 73
SmiMI CAYNNNNRTG 1 cut(s) 235
Sse9I AATT 9 cut(s) 125, 207, 215, 362, 368, 579, 690, 721, 786
SsiI CCGC 3 cut(s) 199, 255, 853
SspI AATATT 2 cut(s) 492, 599
StyD4I CCNGG 1 cut(s) 328
StyI CCWWGG 1 cut(s) 293
TaaI ACNGT 2 cut(s) 591, 658
TaiI ACGT 1 cut(s) 667
TaqI TCGA 1 cut(s) 883
TasI AATT 9 cut(s) 125, 207, 215, 362, 368, 579, 690, 721, 786
TatI WGTACW 2 cut(s) 30, 408
TfiI GAWTC 3 cut(s) 5, 710, 890
Tru1I TTAA 3 cut(s) 219, 365, 774
Tru9I TTAA 3 cut(s) 219, 365, 774
TscAI CASTG 1 cut(s) 22
TspDTI ATGAA 4 cut(s) 570, 723, 794, 924
TspRI CASTG 1 cut(s) 22
VpaK11BI GGWCC 1 cut(s) 73
XapI RAATTY 3 cut(s) 125, 215, 721
XceI RCATGY 2 cut(s) 388, 409
XcmI CCANNNNNNNNNTGG 2 cut(s) 67, 421
ZrmI AGTACT 1 cut(s) 32
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.