Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr04

Orthogroup ID

OG0005530

Physical Location & Seq

Genomic Coordinates Reverse (-)

10323767 .. 10324840

Gene Structure 1074 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD04G1076600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1074 bp

ATGCAAGCTTTCAATTCCAACAACGACGGTGACTATGAAGTTTTTGTCACGGTAGAGAATACAGTTATCCCACTTGACGAGTCGCCTCCGACTCCGCTTCCGCTTCCTCGGATCACGATTCTTTTCAACGTGGTCATAAGCTACAATGTCGATTCGGATATCACCGGGCCGATCAGAGCCTACAATGCAAGGCTCCGACTTGATCACCCTTTTCACTCCTTCACATCCTGTCCACGAGATGCTGTATCCAACATGATCGCCGCCATGCAAATCCCCTTCCCGTTAGATCGTCTCCGGTGGAAAACGCAGAGTTTTGGCGGGAGCACCGAGCCATTAGAGAGCGTGGAAGCCATGATAAGCAAAATAGTTAATGGGGTCAATGCCATGGTGAGTGATGGGAAGCTGGGCATAATTGTTACAATAGAGAAGGAGATTAAAATGGTTGCACACCAAGTGTTTGATGAAATGCAGATGTTGGAAGTTCAGGCCAGGGAACTGCGGGATGAGTTGGAATCAGAAATACTGGGCAACCATCAGGACAATCGTGCATTGCTTGCGATTTACGACGATGGACTTCAACTTCCACAAGAGGGTTGGGCCAGGCAACTGCGTGAAGAGTGGGGATCCGCGGTATCACGGCTTAATCACATTAGTGTTATGCTTTCTGCTGACGATATGCATGGATTGGAGGGTGCTATAGAAACCCTATTGGGACTTCGGGATAGTGTGATAAGAACCTGTGCGAAAATGCGCCTAGTCTTGGCCTTGTTGCGGCAAAATACAAGGGATAGCGATGATAGGTTGTTGACAGAAGCGGTTGTGAGAGAGTCATTAGATCAGGCTGTGTTCAGGCCAATGCCTGCAACCAAAGCATCGGTCGAGGCCTTGAACAAGTTTGTGTTTGATGGTTCGAGTTGTGATCAGCTGTGCGTGGTTTGCTTGGAAAAGATGTTGAGTGGGGATCAAGTCACTTGCTTGCCGTGCTCTCATATTTTTCACGGAGACTGTATCATACAGTGGCTCAACTCGGGTCACACCTGCCCTGTTTGTAGATTCAGATTTCCGACAAACTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000209 GO:0003674 GO:0003824 GO:0004842 GO:0006464 GO:0006508 GO:0006511 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009987 GO:0010498 GO:0016567 GO:0016740 GO:0019538 GO:0019787 GO:0019941 GO:0030163 GO:0032446 GO:0036211 GO:0043161 GO:0043170 GO:0043412 GO:0043632 GO:0044237 GO:0044238 GO:0044248 GO:0044257 GO:0044260 GO:0044265 GO:0044267 GO:0051603 GO:0061630 GO:0061659 GO:0070647 GO:0071704 GO:0140096 GO:1901564 GO:1901565 GO:1901575

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

zf-RING_2

Protein Analysis

358

Amino Acids

39.96

Weight (kDa)

4.98

Isoelectric Point (pI)

44.88

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
zf-rbx1	PF12678	306 - 351	5.6e-07	RING-H2 zinc finger domain
zf-RING_2	PF13639	309 - 351	1.9e-14	Ring finger domain
zf-RING_11	PF17123	310 - 337	3.2e-09	RING-like zinc finger
zf-C3HC4_2	PF13923	310 - 350	3.2e-07	Zinc finger, C3HC4 type (RING finger)
zf-C3HC4	PF00097	310 - 350	4.3e-06	Zinc finger, C3HC4 type (RING finger)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005530)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_3g28951 FvH4_5g00951
malus_domestica	MD04G1076500.v1.1 MD04G1076600.v1.1 MD04G1076700.v1.1
prunus_persica	Prupe.5G082500_v2.0.a1 Prupe.5G082700_v2.0.a1 Prupe.5G082800_v2.0.a1 Prupe.5G083100_v2.0.a1 Prupe.5G083200_v2.0.a1
rosa_chinensis	RchiOBHm_Chr7g0200431
rosa_laevigata	RLG00000003726 RLG00000034854
rosa_multiflora	Rmu_sc0000068.1_g000008 Rmu_sc0000617.1_g000040 Rmu_sc0014686.1_g000001
rosa_roxburghii	Rroxscaffold_3G00256000
rosa_rugosa	Rorug07G0060200
rosa_samantha	Rh5BG360300 Rh5BG360400 Rh5CG385300 Rh5DG375000 Rh5DG375100 Rh7AG185600 Rh7BG188700 Rh7CG195900 Rh7DG189700
rosa_wichuraiana	Rw5G032940 Rw7G016270

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1046
Acc36I	ACCTGC	1 cut(s)	1046
AccII	CGCG	1 cut(s)	629
AciI	CCGC	9 cut(s)	95, 101, 261, 318, 499, 627, 629, 772, 815
AclWI	GGATC	4 cut(s)	119, 618, 631, 969
AdeI	CACNNNGTG	1 cut(s)	454
AfiI	CCNNNNNNNGG	3 cut(s)	590, 760, 771
AgsI	TTSAA	4 cut(s)	13, 127, 578, 889
AjnI	CCWGG	2 cut(s)	488, 599
AleI	CACNNNNGTG	1 cut(s)	651
AluBI	AGCT	4 cut(s)	8, 141, 403, 925
AluI	AGCT	4 cut(s)	8, 141, 403, 925
Alw21I	GWGCWC	2 cut(s)	326, 986
Alw26I	GTCTC	2 cut(s)	296, 996
AlwI	GGATC	4 cut(s)	119, 618, 631, 969
Ama87I	CYCGRG	1 cut(s)	1027
AoxI	GGCC	6 cut(s)	167, 486, 597, 762, 851, 882
ArsI	GACNNNNNNTTYG	2 cut(s)	861, 893
AspLEI	GCGC	1 cut(s)	753
AspS9I	GGNCC	2 cut(s)	167, 597
AsuC2I	CCSGG	1 cut(s)	166
AsuHPI	GGTGA	4 cut(s)	41, 154, 197, 400
AvaI	CYCGRG	1 cut(s)	1027
BamHI	GGATCC	1 cut(s)	623
BauI	CACGAG	1 cut(s)	234
Bbv12I	GWGCWC	2 cut(s)	326, 986
BccI	CCATC	4 cut(s)	389, 540, 563, 899
BceAI	ACGGC	2 cut(s)	653, 964
BciT130I	CCWGG	2 cut(s)	490, 601
BciVI	GTATCC	1 cut(s)	256
BclI	TGATCA	2 cut(s)	202, 919
BcnI	CCSGG	1 cut(s)	166
BcoDI	GTCTC	2 cut(s)	296, 996
BfaI	CTAG	1 cut(s)	755
BfmI	CTRYAG	1 cut(s)	696
BfuAI	ACCTGC	1 cut(s)	1046
BfuI	GTATCC	1 cut(s)	256
BisI	GCNGC	2 cut(s)	261, 773
BlsI	GCNGC	2 cut(s)	262, 774
Bme1390I	CCNGG	3 cut(s)	166, 490, 601
BmeT110I	CYCGRG	1 cut(s)	1027
BmgT120I	GGNCC	2 cut(s)	167, 597
BmiI	GGNNCC	2 cut(s)	194, 625
BmrFI	CCNGG	3 cut(s)	166, 490, 601
BmrI	ACTGGG	1 cut(s)	533
BmsI	GCATC	2 cut(s)	229, 881
BmuI	ACTGGG	1 cut(s)	533
BpuMI	CCSGG	1 cut(s)	166
BsaJI	CCNNGG	4 cut(s)	107, 384, 489, 627
BsaWI	WCCGGW	1 cut(s)	294
Bsc4I	CCNNNNNNNGG	3 cut(s)	590, 760, 771
Bse1I	ACTGG	1 cut(s)	528
Bse3DI	GCAATG	1 cut(s)	548
BseBI	CCWGG	2 cut(s)	490, 601
BseDI	CCNNGG	4 cut(s)	107, 384, 489, 627
BseGI	GGATG	2 cut(s)	224, 508
BseLI	CCNNNNNNNGG	3 cut(s)	590, 760, 771
BseMI	GCAATG	1 cut(s)	548
BseNI	ACTGG	1 cut(s)	528
BseYI	CCCAGC	1 cut(s)	403
Bsh1236I	CGCG	1 cut(s)	629
Bsh1285I	CGRYCG	1 cut(s)	879
BshFI	GGCC	6 cut(s)	169, 488, 599, 764, 853, 884
BsiEI	CGRYCG	1 cut(s)	879
BsiHKAI	GWGCWC	2 cut(s)	326, 986
BsiHKCI	CYCGRG	1 cut(s)	1027
BsiSI	CCGG	2 cut(s)	165, 295
BslFI	GGGAC	1 cut(s)	726
BslI	CCNNNNNNNGG	3 cut(s)	590, 760, 771
BsmAI	GTCTC	2 cut(s)	296, 996
BsmBI	CGTCTC	1 cut(s)	296
BsmFI	GGGAC	1 cut(s)	726
BsnI	GGCC	6 cut(s)	169, 488, 599, 764, 853, 884
BsoBI	CYCGRG	1 cut(s)	1027
Bsp1286I	GDGCHC	2 cut(s)	326, 986
Bsp143I	GATC	9 cut(s)	111, 171, 202, 255, 286, 623, 835, 919, 961
Bsp19I	CCATGG	1 cut(s)	384
BspACI	CCGC	9 cut(s)	95, 101, 261, 318, 499, 627, 629, 772, 815
BspANI	GGCC	6 cut(s)	169, 488, 599, 764, 853, 884
BspFNI	CGCG	1 cut(s)	629
BspLI	GGNNCC	2 cut(s)	194, 625
BspMI	ACCTGC	1 cut(s)	1046
BspPI	GGATC	4 cut(s)	119, 618, 631, 969
BsrDI	GCAATG	1 cut(s)	548
BsrI	ACTGG	1 cut(s)	528
BssECI	CCNNGG	4 cut(s)	107, 384, 489, 627
BssMI	GATC	9 cut(s)	111, 171, 202, 255, 286, 623, 835, 919, 961
BssSI	CACGAG	1 cut(s)	234
BssT1I	CCWWGG	1 cut(s)	384
Bst2BI	CACGAG	1 cut(s)	234
Bst2UI	CCWGG	2 cut(s)	490, 601
Bst4CI	ACNGT	5 cut(s)	29, 52, 64, 1007, 1017
Bst6I	CTCTTC	1 cut(s)	609
BstAPI	GCANNNNNTGC	1 cut(s)	554
BstC8I	GCNNGC	4 cut(s)	6, 555, 861, 977
BstDSI	CCRYGG	2 cut(s)	384, 627
BstF5I	GGATG	2 cut(s)	224, 508
BstFNI	CGCG	1 cut(s)	629
BstHHI	GCGC	1 cut(s)	753
BstKTI	GATC	9 cut(s)	114, 174, 205, 258, 289, 626, 838, 922, 964
BstMAI	GTCTC	2 cut(s)	296, 996
BstMBI	GATC	9 cut(s)	111, 171, 202, 255, 286, 623, 835, 919, 961
BstMCI	CGRYCG	1 cut(s)	879
BstMWI	GCNNNNNNNGC	5 cut(s)	185, 554, 869, 936, 981
BstNI	CCWGG	2 cut(s)	490, 601
BstSCI	CCNGG	3 cut(s)	164, 488, 599
BstSFI	CTRYAG	1 cut(s)	696
BstUI	CGCG	1 cut(s)	629
BstX2I	RGATCY	1 cut(s)	623
BstYI	RGATCY	1 cut(s)	623
BsuI	GTATCC	1 cut(s)	256
BsuRI	GGCC	6 cut(s)	169, 488, 599, 764, 853, 884
BtgI	CCRYGG	2 cut(s)	384, 627
BtgZI	GCGATG	1 cut(s)	807
BtsCI	GGATG	2 cut(s)	224, 508
BtsIMutI	CAGTG	1 cut(s)	1022
BveI	ACCTGC	1 cut(s)	1046
Cac8I	GCNNGC	4 cut(s)	6, 555, 861, 977
CfoI	GCGC	1 cut(s)	753
Cfr13I	GGNCC	2 cut(s)	167, 597
Cfr42I	CCGCGG	1 cut(s)	630
CviAII	CATG	5 cut(s)	253, 265, 352, 385, 680
DpnI	GATC	9 cut(s)	113, 173, 204, 257, 288, 625, 837, 921, 963
DpnII	GATC	9 cut(s)	111, 171, 202, 255, 286, 623, 835, 919, 961
DraIII	CACNNNGTG	1 cut(s)	454
Eam1104I	CTCTTC	1 cut(s)	609
EarI	CTCTTC	1 cut(s)	609
Eco130I	CCWWGG	1 cut(s)	384
Eco147I	AGGCCT	1 cut(s)	884
Eco32I	GATATC	1 cut(s)	160
Eco88I	CYCGRG	1 cut(s)	1027
EcoRII	CCWGG	2 cut(s)	488, 599
EcoRV	GATATC	1 cut(s)	160
EcoT14I	CCWWGG	1 cut(s)	384
EcoT22I	ATGCAT	1 cut(s)	681
ErhI	CCWWGG	1 cut(s)	384
Esp3I	CGTCTC	1 cut(s)	296
FaeI	CATG	5 cut(s)	256, 268, 355, 388, 683
FaqI	GGGAC	1 cut(s)	726
FatI	CATG	5 cut(s)	252, 264, 351, 384, 679
FauI	CCCGC	2 cut(s)	311, 492
FbaI	TGATCA	2 cut(s)	202, 919
Fnu4HI	GCNGC	2 cut(s)	261, 773
FokI	GGATG	2 cut(s)	211, 515
Fsp4HI	GCNGC	2 cut(s)	261, 773
FspBI	CTAG	1 cut(s)	755
GlaI	GCGC	1 cut(s)	752
GluI	GCNGC	2 cut(s)	261, 773
GsaI	CCCAGC	1 cut(s)	407
HaeIII	GGCC	6 cut(s)	169, 488, 599, 764, 853, 884
HapII	CCGG	2 cut(s)	165, 295
HhaI	GCGC	1 cut(s)	753
Hin1II	CATG	5 cut(s)	256, 268, 355, 388, 683
Hin6I	GCGC	1 cut(s)	751
HinP1I	GCGC	1 cut(s)	751
HincII	GTYRAC	1 cut(s)	807
HindII	GTYRAC	1 cut(s)	807
HindIII	AAGCTT	1 cut(s)	6
HinfI	GANTC	7 cut(s)	80, 91, 118, 152, 512, 827, 1053
HpaII	CCGG	2 cut(s)	165, 295
HphI	GGTGA	4 cut(s)	41, 154, 197, 400
Hpy166II	GTNNAC	2 cut(s)	233, 807
Hpy188I	TCNGA	8 cut(s)	90, 111, 157, 176, 197, 517, 1058, 1065
Hpy188III	TCNNGA	3 cut(s)	115, 536, 719
Hpy8I	GTNNAC	2 cut(s)	233, 807
Hpy99I	CGWCG	2 cut(s)	29, 569
HpyAV	CCTTC	3 cut(s)	229, 286, 421
HpyCH4III	ACNGT	5 cut(s)	29, 52, 64, 1007, 1017
HpyCH4IV	ACGT	1 cut(s)	129
HpyCH4V	TGCA	8 cut(s)	4, 188, 268, 446, 469, 548, 679, 863
HpyF10VI	GCNNNNNNNGC	5 cut(s)	185, 554, 869, 936, 981
HpySE526I	ACGT	1 cut(s)	129
Hsp92II	CATG	5 cut(s)	256, 268, 355, 388, 683
HspAI	GCGC	1 cut(s)	751
Ksp22I	TGATCA	2 cut(s)	202, 919
KspI	CCGCGG	1 cut(s)	630
Kzo9I	GATC	9 cut(s)	111, 171, 202, 255, 286, 623, 835, 919, 961
LmnI	GCTCC	2 cut(s)	198, 321
LweI	GCATC	2 cut(s)	229, 881
MaeI	CTAG	1 cut(s)	755
MaeII	ACGT	1 cut(s)	129
MaeIII	GTNAC	5 cut(s)	29, 46, 415, 967, 1031
MalI	GATC	9 cut(s)	113, 173, 204, 257, 288, 625, 837, 921, 963
MboI	GATC	9 cut(s)	111, 171, 202, 255, 286, 623, 835, 919, 961
MboII	GAAGA	1 cut(s)	626
MflI	RGATCY	1 cut(s)	623
MhlI	GDGCHC	2 cut(s)	326, 986
MluCI	AATT	2 cut(s)	13, 411
MlyI	GAGTC	3 cut(s)	85, 89, 836
MmeI	TCCRAC	6 cut(s)	42, 113, 220, 273, 456, 489
MnlI	CCTC	5 cut(s)	96, 117, 583, 682, 874
Mph1103I	ATGCAT	1 cut(s)	681
MseI	TTAA	3 cut(s)	369, 435, 642
MslI	CAYNNNNRTG	1 cut(s)	651
MspA1I	CMGCKG	2 cut(s)	629, 925
MspI	CCGG	2 cut(s)	165, 295
MspR9I	CCNGG	3 cut(s)	166, 490, 601
MvaI	CCWGG	2 cut(s)	490, 601
MvnI	CGCG	1 cut(s)	629
MwoI	GCNNNNNNNGC	5 cut(s)	185, 554, 869, 936, 981
NciI	CCSGG	1 cut(s)	166
NcoI	CCATGG	1 cut(s)	384
NdeII	GATC	9 cut(s)	111, 171, 202, 255, 286, 623, 835, 919, 961
NlaIII	CATG	5 cut(s)	256, 268, 355, 388, 683
NlaIV	GGNNCC	2 cut(s)	194, 625
NmuCI	GTSAC	4 cut(s)	29, 46, 967, 1031
NsiI	ATGCAT	1 cut(s)	681
OliI	CACNNNNGTG	1 cut(s)	651
PaqCI	CACCTGC	1 cut(s)	1046
PceI	AGGCCT	1 cut(s)	884
PfeI	GAWTC	4 cut(s)	118, 152, 512, 1053
PkrI	GCNGC	2 cut(s)	262, 774
PleI	GAGTC	3 cut(s)	85, 88, 835
PpsI	GAGTC	3 cut(s)	85, 88, 835
Psp6I	CCWGG	2 cut(s)	488, 599
PspFI	CCCAGC	1 cut(s)	403
PspGI	CCWGG	2 cut(s)	488, 599
PspN4I	GGNNCC	2 cut(s)	194, 625
PspPI	GGNCC	2 cut(s)	167, 597
PsuI	RGATCY	1 cut(s)	623
PvuII	CAGCTG	1 cut(s)	925
RseI	CAYNNNNRTG	1 cut(s)	651
SacII	CCGCGG	1 cut(s)	630
SaqAI	TTAA	3 cut(s)	369, 435, 642
SatI	GCNGC	2 cut(s)	261, 773
Sau3AI	GATC	9 cut(s)	111, 171, 202, 255, 286, 623, 835, 919, 961
Sau96I	GGNCC	2 cut(s)	167, 597
SchI	GAGTC	3 cut(s)	85, 89, 836
ScrFI	CCNGG	3 cut(s)	166, 490, 601
SduI	GDGCHC	2 cut(s)	326, 986
SetI	ASST	8 cut(s)	10, 132, 143, 405, 740, 803, 927, 1040
SfaNI	GCATC	2 cut(s)	229, 881
SfcI	CTRYAG	1 cut(s)	696
Sfr303I	CCGCGG	1 cut(s)	630
SgrBI	CCGCGG	1 cut(s)	630
SmiMI	CAYNNNNRTG	1 cut(s)	651
Sse9I	AATT	2 cut(s)	13, 411
SseBI	AGGCCT	1 cut(s)	884
SsiI	CCGC	9 cut(s)	95, 101, 261, 318, 499, 627, 629, 772, 815
SspMI	CTAG	1 cut(s)	755
StuI	AGGCCT	1 cut(s)	884
StyD4I	CCNGG	3 cut(s)	164, 488, 599
StyI	CCWWGG	1 cut(s)	384
TaaI	ACNGT	5 cut(s)	29, 52, 64, 1007, 1017
TaiI	ACGT	1 cut(s)	132
TaqI	TCGA	3 cut(s)	150, 879, 911
TaqII	GACCGA	1 cut(s)	865
TasI	AATT	2 cut(s)	13, 411
TauI	GCSGC	2 cut(s)	263, 775
TfiI	GAWTC	4 cut(s)	118, 152, 512, 1053
Tru1I	TTAA	3 cut(s)	369, 435, 642
Tru9I	TTAA	3 cut(s)	369, 435, 642
TscAI	CASTG	1 cut(s)	1022
TseFI	GTSAC	4 cut(s)	29, 46, 967, 1031
Tsp45I	GTSAC	4 cut(s)	29, 46, 967, 1031
TspDTI	ATGAA	2 cut(s)	51, 477
TspGWI	ACGGA	1 cut(s)	1014
TspRI	CASTG	1 cut(s)	1022
XcmI	CCANNNNNNNNNTGG	1 cut(s)	591
XspI	CTAG	1 cut(s)	755
Zsp2I	ATGCAT	1 cut(s)	681

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.