MD04G1076500.v1.1

Ring finger domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005530
Physical Location & Seq
Reverse (-)
10320684 .. 10321421
738 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1076500.v1.1.491

Sequence Viewer

Length: 738 bp
ATGACATGCCTTTGCCATTGGATAAGCCACGATGGAAGAAGGGACGTGGTTAAACACACTGATGATCCACCAAACGGAGTTGATGATTTGATCAGCAACATATGTCACTCCGTTAAGGATATGCTTGTTGCAGGACGCAAGAAGCTGGTCGTGAGGATCAAGATTGAGAAGCAGATTAGTACGTCAAATCAACAATATGATACGATTAACATAAGAAGAGACCTCGAACACAAATTAAAGGATTTCCTTTCTGTGCTTTCGCGTTCAGGTTTGCTACAACAGCCACGTGACGAGGTGCACAGCCAAGCTTCGATGAGATCCGCTATGGCGTATTGGAATGAAATTAAGTCACGCGCAGCCGCTGCAGGGTTGATCAATGATATTAACAGTGCCAGTACTAGTCACAGAGGAAATTATGGAGTAGAGGATGTTAATGCATTACTGCTAGAGATAAGTATCGTGATGGAGGCTGCATCAGGTGAATATCAATCGTCACAGTCTAGGCCTAAGCCGGCGAGCAAGGTGTTCGTTGAGGCCTTGGACGCGTTTAAAATAACCGATGGAGTAAATCATGTTACGAATGCTGAATTATGTGTGGTTTGCTTGGAGAAGATTTTGAGTGGGGAAGAAGTTACGCCCTTGCCATGCTCTCATATGTTTCATGCAAAATGTGTTGTTCAATGGTTCAAATCCGGTCATACGTGCCCTGTGTGTAGGTTCGAGTGTCCGACTGATTGA

Protein Analysis

246

Amino Acids

27.29

Weight (kDa)

6.59

Isoelectric Point (pI)

41.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-RING_2 PF13639 197 - 239 1.3e-14 Ring finger domain
zf-RING_11 PF17123 198 - 225 2.9e-10 RING-like zinc finger
zf-C3HC4_2 PF13923 198 - 238 6.5e-07 Zinc finger, C3HC4 type (RING finger)
zf-C3HC4 PF00097 198 - 238 1e-05 Zinc finger, C3HC4 type (RING finger)
zf-rbx1 PF12678 204 - 239 6.6e-07 RING-H2 zinc finger domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 262, 354, 545
AciI CCGC 2 cut(s) 321, 360
AclWI GGATC 3 cut(s) 59, 164, 312
AcvI CACGTG 1 cut(s) 287
AfaI GTAC 2 cut(s) 181, 397
AfiI CCNNNNNNNGG 2 cut(s) 74, 366
AflIII ACRYGT 1 cut(s) 543
AgsI TTSAA 2 cut(s) 680, 688
AhlI ACTAGT 1 cut(s) 398
AjiI CACGTC 1 cut(s) 46
AluBI AGCT 2 cut(s) 145, 308
AluI AGCT 2 cut(s) 145, 308
Alw21I GWGCWC 1 cut(s) 300
Alw26I GTCTC 1 cut(s) 213
Alw44I GTGCAC 1 cut(s) 296
AlwI GGATC 3 cut(s) 59, 164, 312
AlwNI CAGNNNCTG 1 cut(s) 362
AoxI GGCC 2 cut(s) 503, 534
ApaLI GTGCAC 1 cut(s) 296
ApeKI GCWGC 3 cut(s) 356, 362, 470
AspLEI GCGC 1 cut(s) 356
AsuHPI GGTGA 1 cut(s) 491
BaeGI GKGCMC 2 cut(s) 300, 707
BbrPI CACGTG 1 cut(s) 287
Bbv12I GWGCWC 1 cut(s) 300
BbvI GCAGC 3 cut(s) 349, 368, 457
BccI CCATC 3 cut(s) 26, 457, 554
BcgI CGANNNNNNTGC 2 cut(s) 508, 542
BclI TGATCA 2 cut(s) 90, 372
BcoDI GTCTC 1 cut(s) 213
BcuI ACTAGT 1 cut(s) 398
BfaI CTAG 3 cut(s) 399, 446, 501
BfmI CTRYAG 1 cut(s) 363
BisI GCNGC 4 cut(s) 357, 360, 363, 471
BlsI GCNGC 4 cut(s) 358, 361, 364, 472
BmcAI AGTACT 1 cut(s) 397
BmgBI CACGTC 1 cut(s) 46
BmsI GCATC 1 cut(s) 482
Bpu10I CCTNAGC 1 cut(s) 507
BsaAI YACGTR 2 cut(s) 287, 702
BsaBI GATNNNNATC 1 cut(s) 455
BsaI GGTCTC 1 cut(s) 213
BsaJI CCNNGG 1 cut(s) 537
BsaWI WCCGGW 1 cut(s) 692
Bsc4I CCNNNNNNNGG 2 cut(s) 74, 366
Bse118I RCCGGY 1 cut(s) 511
Bse1I ACTGG 1 cut(s) 393
Bse8I GATNNNNATC 1 cut(s) 455
BseDI CCNNGG 1 cut(s) 537
BseGI GGATG 1 cut(s) 433
BseJI GATNNNNATC 1 cut(s) 455
BseLI CCNNNNNNNGG 2 cut(s) 74, 366
BseNI ACTGG 1 cut(s) 393
BseSI GKGCMC 2 cut(s) 300, 707
BseXI GCAGC 3 cut(s) 349, 368, 457
Bsh1236I CGCG 3 cut(s) 262, 354, 545
BshFI GGCC 2 cut(s) 505, 536
BsiHKAI GWGCWC 1 cut(s) 300
BsiSI CCGG 2 cut(s) 512, 693
BslFI GGGAC 1 cut(s) 56
BslI CCNNNNNNNGG 2 cut(s) 74, 366
BsmAI GTCTC 1 cut(s) 213
BsmFI GGGAC 1 cut(s) 56
BsmI GAATGC 1 cut(s) 586
BsnI GGCC 2 cut(s) 505, 536
Bso31I GGTCTC 1 cut(s) 213
Bsp1286I GDGCHC 2 cut(s) 300, 707
Bsp143I GATC 5 cut(s) 64, 90, 156, 317, 372
BspACI CCGC 2 cut(s) 321, 360
BspANI GGCC 2 cut(s) 505, 536
BspFNI CGCG 3 cut(s) 262, 354, 545
BspMAI CTGCAG 1 cut(s) 367
BspPI GGATC 3 cut(s) 59, 164, 312
BspTNI GGTCTC 1 cut(s) 213
BsrFI RCCGGY 1 cut(s) 511
BsrI ACTGG 1 cut(s) 393
BssAI RCCGGY 1 cut(s) 511
BssECI CCNNGG 1 cut(s) 537
BssMI GATC 5 cut(s) 64, 90, 156, 317, 372
BssT1I CCWWGG 1 cut(s) 537
Bst4CI ACNGT 2 cut(s) 389, 498
Bst6I CTCTTC 1 cut(s) 211
BstAPI GCANNNNNTGC 1 cut(s) 362
BstBAI YACGTR 2 cut(s) 287, 702
BstC8I GCNNGC 2 cut(s) 513, 517
BstDEI CTNAG 1 cut(s) 507
BstF5I GGATG 1 cut(s) 433
BstFNI CGCG 3 cut(s) 262, 354, 545
BstHHI GCGC 1 cut(s) 356
BstKTI GATC 5 cut(s) 67, 93, 159, 320, 375
BstMAI GTCTC 1 cut(s) 213
BstMBI GATC 5 cut(s) 64, 90, 156, 317, 372
BstMWI GCNNNNNNNGC 3 cut(s) 280, 362, 542
BstNSI RCATGY 1 cut(s) 9
BstSFI CTRYAG 1 cut(s) 363
BstSLI GKGCMC 2 cut(s) 300, 707
BstUI CGCG 3 cut(s) 262, 354, 545
BstV1I GCAGC 3 cut(s) 349, 368, 457
BstX2I RGATCY 1 cut(s) 317
BstYI RGATCY 1 cut(s) 317
BsuRI GGCC 2 cut(s) 505, 536
BtrI CACGTC 1 cut(s) 46
BtsCI GGATG 1 cut(s) 433
BtsIMutI CAGTG 2 cut(s) 57, 394
Cac8I GCNNGC 2 cut(s) 513, 517
CaiI CAGNNNCTG 1 cut(s) 362
CfoI GCGC 1 cut(s) 356
Cfr10I RCCGGY 1 cut(s) 511
CseI GACGC 2 cut(s) 144, 551
Csp6I GTAC 2 cut(s) 180, 396
CviAII CATG 4 cut(s) 6, 572, 645, 662
CviQI GTAC 2 cut(s) 180, 396
DdeI CTNAG 1 cut(s) 507
DpnI GATC 5 cut(s) 66, 92, 158, 319, 374
DpnII GATC 5 cut(s) 64, 90, 156, 317, 372
DraI TTTAAA 1 cut(s) 550
Eam1104I CTCTTC 1 cut(s) 211
EarI CTCTTC 1 cut(s) 211
Eco130I CCWWGG 1 cut(s) 537
Eco147I AGGCCT 2 cut(s) 505, 536
Eco31I GGTCTC 1 cut(s) 213
Eco72I CACGTG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 537
EcoT22I ATGCAT 1 cut(s) 439
ErhI CCWWGG 1 cut(s) 537
FaeI CATG 4 cut(s) 9, 575, 648, 665
FaqI GGGAC 1 cut(s) 56
FatI CATG 4 cut(s) 5, 571, 644, 661
FauNDI CATATG 2 cut(s) 101, 654
FbaI TGATCA 2 cut(s) 90, 372
Fnu4HI GCNGC 4 cut(s) 357, 360, 363, 471
FokI GGATG 1 cut(s) 440
Fsp4HI GCNGC 4 cut(s) 357, 360, 363, 471
FspBI CTAG 3 cut(s) 399, 446, 501
GlaI GCGC 1 cut(s) 355
GluI GCNGC 4 cut(s) 357, 360, 363, 471
HaeIII GGCC 2 cut(s) 505, 536
HapII CCGG 2 cut(s) 512, 693
HgaI GACGC 2 cut(s) 144, 551
HhaI GCGC 1 cut(s) 356
Hin1II CATG 4 cut(s) 9, 575, 648, 665
Hin6I GCGC 1 cut(s) 354
HinP1I GCGC 1 cut(s) 354
HindIII AAGCTT 1 cut(s) 306
HpaII CCGG 2 cut(s) 512, 693
HphI GGTGA 1 cut(s) 491
Hpy166II GTNNAC 1 cut(s) 298
Hpy188I TCNGA 1 cut(s) 729
Hpy188III TCNNGA 3 cut(s) 151, 160, 460
Hpy8I GTNNAC 1 cut(s) 298
HpyAV CCTTC 1 cut(s) 33
HpyCH4III ACNGT 2 cut(s) 389, 498
HpyCH4IV ACGT 4 cut(s) 45, 182, 286, 701
HpyCH4V TGCA 6 cut(s) 131, 298, 365, 437, 473, 665
HpyF10VI GCNNNNNNNGC 3 cut(s) 280, 362, 542
HpyF3I CTNAG 1 cut(s) 507
HpySE526I ACGT 4 cut(s) 45, 182, 286, 701
Hsp92II CATG 4 cut(s) 9, 575, 648, 665
HspAI GCGC 1 cut(s) 354
KroI GCCGGC 1 cut(s) 511
KroNI GCCGGC 1 cut(s) 513
Ksp22I TGATCA 2 cut(s) 90, 372
Kzo9I GATC 5 cut(s) 64, 90, 156, 317, 372
LpnPI CCDG 9 cut(s) 117, 131, 252, 351, 406, 462, 525, 706, 720
Lsp1109I GCAGC 3 cut(s) 349, 368, 457
LweI GCATC 1 cut(s) 482
MaeI CTAG 3 cut(s) 399, 446, 501
MaeII ACGT 4 cut(s) 45, 182, 286, 701
MaeIII GTNAC 7 cut(s) 104, 287, 348, 401, 492, 574, 631
MalI GATC 5 cut(s) 66, 92, 158, 319, 374
MboI GATC 5 cut(s) 64, 90, 156, 317, 372
MboII GAAGA 4 cut(s) 48, 228, 622, 638
MflI RGATCY 1 cut(s) 317
MhlI GDGCHC 2 cut(s) 300, 707
MluCI AATT 4 cut(s) 233, 342, 412, 587
MluI ACGCGT 1 cut(s) 543
MnlI CCTC 7 cut(s) 147, 233, 286, 401, 418, 460, 526
Mph1103I ATGCAT 1 cut(s) 439
MroNI GCCGGC 1 cut(s) 511
MseI TTAA 8 cut(s) 51, 114, 207, 236, 345, 384, 432, 549
MslI CAYNNNNRTG 1 cut(s) 60
MspA1I CMGCKG 1 cut(s) 362
MspI CCGG 2 cut(s) 512, 693
Mva1269I GAATGC 1 cut(s) 586
MvnI CGCG 3 cut(s) 262, 354, 545
MwoI GCNNNNNNNGC 3 cut(s) 280, 362, 542
NaeI GCCGGC 1 cut(s) 513
NdeI CATATG 2 cut(s) 101, 654
NdeII GATC 5 cut(s) 64, 90, 156, 317, 372
NgoMIV GCCGGC 1 cut(s) 511
NlaIII CATG 4 cut(s) 9, 575, 648, 665
NmuCI GTSAC 5 cut(s) 104, 287, 348, 401, 492
NsiI ATGCAT 1 cut(s) 439
NspI RCATGY 1 cut(s) 9
PceI AGGCCT 2 cut(s) 505, 536
PctI GAATGC 1 cut(s) 586
PdiI GCCGGC 1 cut(s) 513
PkrI GCNGC 4 cut(s) 358, 361, 364, 472
PmaCI CACGTG 1 cut(s) 287
PmlI CACGTG 1 cut(s) 287
Ppu21I YACGTR 2 cut(s) 287, 702
PspCI CACGTG 1 cut(s) 287
PstI CTGCAG 1 cut(s) 367
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 1 cut(s) 317
RsaI GTAC 2 cut(s) 181, 397
RsaNI GTAC 2 cut(s) 180, 396
RseI CAYNNNNRTG 1 cut(s) 60
SaqAI TTAA 8 cut(s) 51, 114, 207, 236, 345, 384, 432, 549
SatI GCNGC 4 cut(s) 357, 360, 363, 471
Sau3AI GATC 5 cut(s) 64, 90, 156, 317, 372
ScaI AGTACT 1 cut(s) 397
SduI GDGCHC 2 cut(s) 300, 707
SfaNI GCATC 1 cut(s) 482
SfcI CTRYAG 1 cut(s) 363
SmiMI CAYNNNNRTG 1 cut(s) 60
SpeI ACTAGT 1 cut(s) 398
Sse9I AATT 4 cut(s) 233, 342, 412, 587
SseBI AGGCCT 2 cut(s) 505, 536
SsiI CCGC 2 cut(s) 321, 360
SspMI CTAG 3 cut(s) 399, 446, 501
StuI AGGCCT 2 cut(s) 505, 536
StyI CCWWGG 1 cut(s) 537
TaaI ACNGT 2 cut(s) 389, 498
TaiI ACGT 4 cut(s) 48, 185, 289, 704
TaqI TCGA 3 cut(s) 225, 311, 720
TasI AATT 4 cut(s) 233, 342, 412, 587
TatI WGTACW 1 cut(s) 395
TauI GCSGC 1 cut(s) 362
Tru1I TTAA 8 cut(s) 51, 114, 207, 236, 345, 384, 432, 549
Tru9I TTAA 8 cut(s) 51, 114, 207, 236, 345, 384, 432, 549
TscAI CASTG 2 cut(s) 64, 394
TseFI GTSAC 5 cut(s) 104, 287, 348, 401, 492
TseI GCWGC 3 cut(s) 356, 362, 470
Tsp45I GTSAC 5 cut(s) 104, 287, 348, 401, 492
TspDTI ATGAA 2 cut(s) 354, 650
TspGWI ACGGA 2 cut(s) 90, 100
TspRI CASTG 2 cut(s) 64, 394
VneI GTGCAC 1 cut(s) 296
XceI RCATGY 1 cut(s) 9
XspI CTAG 3 cut(s) 399, 446, 501
ZrmI AGTACT 1 cut(s) 397
Zsp2I ATGCAT 1 cut(s) 439
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.