MD00G1078200.v1.1

Protein EPIDERMAL PATTERNING FACTOR

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0018309
Physical Location & Seq
Reverse (-)
15578900 .. 15579859
960 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1078200.v1.1.491

Sequence Viewer

Length: 378 bp
ATGAAGAAGAGATTGTCTGTTGGAGTTGCAGCCCACACAAGTTTCCTCCTTGTGGTTTTCTTCATGCTAATTACGATAGGCAGAAGCCTACGTGTTTGGCCTCCTCACGTCCCTCTAAACCTTCAAAGAGAAGTTGCAGCCGGTAACAGAGAGAACCTGTACCGAAGCGAGGAAGGAGACGAGGAAAGAGGAAAGGAAGAAGAGAGGAAGGACCTATACCAAAGCGGATCGAGGTTGCCGGACTGTTCACATGCGTGCGGACCGTGCTTTCCATGTAAAAGAGTGATGGTGAGCTTCAAGTGCTCCACTTCAGAGTCATGCCCAATAGTCTACAGGTGCATGTGTAAAGGGAAGTATTACCATGTTCCCTCTAACTAG

Protein Analysis

126

Amino Acids

14.25

Weight (kDa)

8.97

Isoelectric Point (pI)

55.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
EPF PF17181 75 - 122 1.3e-14 Epidermal patterning factor proteins
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0018309)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G34245
fragaria_vesca FvH4_2g12460
malus_domestica MD00G1078200.v1.1
prunus_persica Prupe.8G050100_v2.0.a1
rosa_chinensis RchiOBHm_Chr6g0272891
rosa_laevigata RLG00000013657
rosa_roxburghii Rroxscaffold_7G00195940
rosa_samantha Rh6AG186900 Rh6DG181000
rosa_wichuraiana Rw6G016180

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 330
AciI CCGC 2 cut(s) 225, 258
AclWI GGATC 1 cut(s) 235
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 161
AfiI CCNNNNNNNGG 2 cut(s) 52, 169
AflIII ACRYGT 1 cut(s) 91
AgsI TTSAA 2 cut(s) 125, 298
AjiI CACGTC 1 cut(s) 109
AleI CACNNNNGTG 1 cut(s) 253
AluBI AGCT 1 cut(s) 294
AluI AGCT 1 cut(s) 294
Alw21I GWGCWC 1 cut(s) 305
Alw26I GTCTC 1 cut(s) 171
AlwI GGATC 1 cut(s) 235
AoxI GGCC 1 cut(s) 98
ApeKI GCWGC 2 cut(s) 29, 137
AspS9I GGNCC 2 cut(s) 211, 260
AsuHPI GGTGA 1 cut(s) 301
AvaII GGWCC 2 cut(s) 211, 260
BarI GAAGNNNNNNTAC 2 cut(s) 200, 232
Bbv12I GWGCWC 1 cut(s) 305
BbvI GCAGC 2 cut(s) 41, 149
BccI CCATC 1 cut(s) 280
BcoDI GTCTC 1 cut(s) 171
BfaI CTAG 1 cut(s) 376
BfmI CTRYAG 1 cut(s) 331
BisI GCNGC 2 cut(s) 30, 138
BlsI GCNGC 2 cut(s) 31, 139
Bme18I GGWCC 2 cut(s) 211, 260
BmgBI CACGTC 1 cut(s) 109
BmgT120I GGNCC 2 cut(s) 211, 260
BsaAI YACGTR 1 cut(s) 92
Bsc4I CCNNNNNNNGG 2 cut(s) 52, 169
Bse118I RCCGGY 1 cut(s) 140
BseLI CCNNNNNNNGG 2 cut(s) 52, 169
BseRI GAGGAG 1 cut(s) 93
BseXI GCAGC 2 cut(s) 41, 149
BshFI GGCC 1 cut(s) 100
BsiHKAI GWGCWC 1 cut(s) 305
BsiSI CCGG 2 cut(s) 141, 239
BslFI GGGAC 1 cut(s) 95
BslI CCNNNNNNNGG 2 cut(s) 52, 169
BsmAI GTCTC 1 cut(s) 171
BsmBI CGTCTC 1 cut(s) 171
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 1 cut(s) 100
Bsp1286I GDGCHC 1 cut(s) 305
Bsp143I GATC 1 cut(s) 227
BspACI CCGC 2 cut(s) 225, 258
BspANI GGCC 1 cut(s) 100
BspPI GGATC 1 cut(s) 235
BsrFI RCCGGY 1 cut(s) 140
BssAI RCCGGY 1 cut(s) 140
BssMI GATC 1 cut(s) 227
Bst4CI ACNGT 2 cut(s) 245, 264
Bst6I CTCTTC 2 cut(s) 2, 195
BstBAI YACGTR 1 cut(s) 92
BstC8I GCNNGC 1 cut(s) 256
BstKTI GATC 1 cut(s) 230
BstMAI GTCTC 1 cut(s) 171
BstMBI GATC 1 cut(s) 227
BstMWI GCNNNNNNNGC 2 cut(s) 264, 300
BstNSI RCATGY 2 cut(s) 254, 343
BstSFI CTRYAG 1 cut(s) 331
BstV1I GCAGC 2 cut(s) 41, 149
BsuRI GGCC 1 cut(s) 100
BtrI CACGTC 1 cut(s) 109
Cac8I GCNNGC 1 cut(s) 256
Cfr10I RCCGGY 1 cut(s) 140
Cfr13I GGNCC 2 cut(s) 211, 260
CpoI CGGWCCG 1 cut(s) 260
Csp6I GTAC 1 cut(s) 160
CspI CGGWCCG 1 cut(s) 260
CviAII CATG 6 cut(s) 64, 251, 273, 318, 340, 362
CviJI RGCY 5 cut(s) 32, 87, 100, 140, 294
CviKI_1 RGCY 5 cut(s) 32, 87, 100, 140, 294
CviQI GTAC 1 cut(s) 160
DpnI GATC 1 cut(s) 229
DpnII GATC 1 cut(s) 227
Eam1104I CTCTTC 2 cut(s) 2, 195
EarI CTCTTC 2 cut(s) 2, 195
Eco47I GGWCC 2 cut(s) 211, 260
Eco57I CTGAAG 1 cut(s) 294
EcoO109I RGGNCCY 1 cut(s) 211
Esp3I CGTCTC 1 cut(s) 171
FaeI CATG 6 cut(s) 67, 254, 276, 321, 343, 365
FaiI YATR 7 cut(s) 65, 217, 252, 274, 319, 341, 363
FaqI GGGAC 1 cut(s) 95
FatI CATG 6 cut(s) 63, 250, 272, 317, 339, 361
FblI GTMKAC 1 cut(s) 330
Fnu4HI GCNGC 2 cut(s) 30, 138
Fsp4HI GCNGC 2 cut(s) 30, 138
FspBI CTAG 1 cut(s) 376
GluI GCNGC 2 cut(s) 30, 138
HaeIII GGCC 1 cut(s) 100
HapII CCGG 2 cut(s) 141, 239
Hin1II CATG 6 cut(s) 67, 254, 276, 321, 343, 365
HinfI GANTC 1 cut(s) 314
HpaII CCGG 2 cut(s) 141, 239
HphI GGTGA 1 cut(s) 301
Hpy166II GTNNAC 2 cut(s) 248, 331
Hpy188I TCNGA 1 cut(s) 313
Hpy8I GTNNAC 2 cut(s) 248, 331
HpyAV CCTTC 3 cut(s) 131, 167, 202
HpyCH4III ACNGT 2 cut(s) 245, 264
HpyCH4IV ACGT 2 cut(s) 91, 108
HpyCH4V TGCA 3 cut(s) 29, 137, 339
HpyF10VI GCNNNNNNNGC 2 cut(s) 264, 300
HpySE526I ACGT 2 cut(s) 91, 108
Hsp92II CATG 6 cut(s) 67, 254, 276, 321, 343, 365
Kzo9I GATC 1 cut(s) 227
LmnI GCTCC 1 cut(s) 308
LpnPI CCDG 4 cut(s) 154, 170, 252, 319
Lsp1109I GCAGC 2 cut(s) 41, 149
MaeI CTAG 1 cut(s) 376
MaeII ACGT 2 cut(s) 91, 108
MaeIII GTNAC 1 cut(s) 143
MalI GATC 1 cut(s) 229
MboI GATC 1 cut(s) 227
MboII GAAGA 5 cut(s) 16, 19, 52, 209, 212
MhlI GDGCHC 1 cut(s) 305
MluCI AATT 1 cut(s) 69
MlyI GAGTC 1 cut(s) 323
MnlI CCTC 9 cut(s) 56, 111, 114, 123, 163, 175, 182, 198, 225
MslI CAYNNNNRTG 1 cut(s) 253
MspI CCGG 2 cut(s) 141, 239
MwoI GCNNNNNNNGC 2 cut(s) 264, 300
NdeII GATC 1 cut(s) 227
NlaIII CATG 6 cut(s) 67, 254, 276, 321, 343, 365
NspI RCATGY 2 cut(s) 254, 343
OliI CACNNNNGTG 1 cut(s) 253
PkrI GCNGC 2 cut(s) 31, 139
PleI GAGTC 1 cut(s) 322
PpsI GAGTC 1 cut(s) 322
Ppu21I YACGTR 1 cut(s) 92
PpuMI RGGWCCY 1 cut(s) 211
Psp5II RGGWCCY 1 cut(s) 211
PspPI GGNCC 2 cut(s) 211, 260
PspPPI RGGWCCY 1 cut(s) 211
RsaI GTAC 1 cut(s) 161
RsaNI GTAC 1 cut(s) 160
RseI CAYNNNNRTG 1 cut(s) 253
Rsr2I CGGWCCG 1 cut(s) 260
RsrII CGGWCCG 1 cut(s) 260
SatI GCNGC 2 cut(s) 30, 138
Sau3AI GATC 1 cut(s) 227
Sau96I GGNCC 2 cut(s) 211, 260
SchI GAGTC 1 cut(s) 323
SduI GDGCHC 1 cut(s) 305
SetI ASST 8 cut(s) 94, 111, 123, 159, 216, 236, 296, 338
SfcI CTRYAG 1 cut(s) 331
SinI GGWCC 2 cut(s) 211, 260
SmiMI CAYNNNNRTG 1 cut(s) 253
Sse9I AATT 1 cut(s) 69
SsiI CCGC 2 cut(s) 225, 258
SspMI CTAG 1 cut(s) 376
TaaI ACNGT 2 cut(s) 245, 264
TaiI ACGT 2 cut(s) 94, 111
TaqI TCGA 1 cut(s) 230
TasI AATT 1 cut(s) 69
TseI GCWGC 2 cut(s) 29, 137
TspDTI ATGAA 2 cut(s) 17, 52
VpaK11BI GGWCC 2 cut(s) 211, 260
XceI RCATGY 2 cut(s) 254, 343
XmiI GTMKAC 1 cut(s) 330
XspI CTAG 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.