Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0003724

Physical Location & Seq

Genomic Coordinates Forward (+)

17507835 .. 17514646

Gene Structure 6812 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1086100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2787 bp

ATGGGGGCAATTGGTGGGGAAGATCTAGTGAAGTGGGAGAAGATGCAAGGAGCAACTGCTCGCGAAGAGAAGATTCTGGTTTTGGTGAGGTTGAGGCCTCTGAGTGAGAAGGAGGTTGCAGCAAGTGAAGTAGCAGATTGGGAATGCATCAATGACACAACCATCTTATACCGAAACACGCTGCGGGAAGGGTCTACATTTCCAACTGCCTATACATTTGACAAAGTATTTCGGGGTGATTGCTCTACAAGGCAGGTTTATGATGAAGGAGCCCAACAAATTGCTCTTTCAGTTGTCAATGGTATCAACTCAAGTATTTTTGCATATGGTCAAACAAGCAGTGGGAAAACGTACACTATGAATGGTATAACAGAGTTTACAGTAGCAGAAATATTTGATTATGTACATGCGCATGAGGAACGAGCTTTTGTTGTTAAGTTTTCAGCTATTGAGATATACAATGAAGCTGTTAGAGACCTCCTAAGCACAGATAATACTCCGCTTAGGCTGCTAGATGATCCTGAGCGGGGAACCATAATAGAGAAAATCACAGAAGAAACTCTGAGGGACTGGAACCATTTAAAGGAGCTCCTTTCTATGTGCGAAGCTCAAAGACAGATAGGGGAGACTGCATTAAATGAAAAAAGCTCTAGATCACATCAAATTATTAAGCTGGCAATTGAAAGTTCTGCTCGTGAGTTCCTAGGCAAAGGCAATTCAACCACCCTTTCAGCTAGCGTGAATTTTGTGGACTTGGCAGGAAGCGAGCGTGCAGCCCAGGCATTATCAGCTGGGATGAGATTAAAAGAAGGCTGCCACATTAATCGCAGTTTACTAACTCTTAGCACTGTTATTCGCAAGCTAAGTAAAGGAAGGAATGGCCACATCAATTACAGAGATTCTAAGCTAACACGCATACTGCAGCCGGCCTTGGGTGGTAATGCTAGAACTGCCATCATTTGCACTTTGAGCCCTGCACGAAGCCATGTTGAGCAAACAAGAAACACTCTTTTGTTTGCTTGTTGTGCAAAAGAAGTGACTACAAAAGCACAGGTCAATGTAGTCATGTCAGATAAGGCCCTTGTTAAACATTTACAGAAAGAGCTGGCTAGATTGGAGAGTGAGTTGAGGATTCCTGGCCCCCCTTCCTCAACTTGTGATTACCCAGCGTTGCTTAGAAAGAAAGATCTTCAGATTGAAAAGATGGACAAGGAAATTAGAGAGCTGAAAAAGCAATGTGATCTTGCTGAATCACGGGTGGAGGATTTACTGCGGATGGTTGGAAAGGATAATGATTCCAGACAACCTTCAGATAATCTCAATCCTAAATGGAACGCTGGGGATGTGTCAGATGATGAAGGCTCAGTATCATCAGGTGTGGCTGATCCTTATTATATGAATGGCATCAGAAAGTTCAACAATCCTCATTTTGATGACAGAGACGGTGAGAGCAGTCCCGAGGAGGTATACGGACAGATTCTTGAGAAATTTAGATCTGATTCATGCCAGAGTGTAGAGGATCCCACAGGTGGGACTGCAGAAGATACTGATGACTACTGCAAAGAAGTCCGCTGTATTGAGATGGAAGAGTCAAGCAGGGACAAGAATTCTGGATCCCTTGCTTTATCCACTGTTGAAAATGAAGTAACATCTGGGGATGCAAGTTTAGCAGGTCAAGAAATGATATCAACCCCAGTGAATGCAGATAGAGAAGGGAGTCAGATGCAAAATGGTTTTGCATATGGCACGTTCGAACAGAGACTACGTGATGTACAATTGACGATTGATTCTATTGGTAGTCCTTACCCTGAAGAACAATCTAGAAGCCTAAAGTTAACTAGGAGTTGGAGTTGTAGAGAAAATTTCACGGCTGGTTCATCTTCACCGGATAAATCGGAGAGAACCCCACCTAATTGGTTGGAGAAAAGCTTTCCTGGGAGACCAGAAGGTTTTAGCGGGAGGAAGGTTCCATTATTACATTATGATTCCAGCGCAAGGTTGTCAAGAAATGATTCTCAATCTTCCCTTGGGAGTGCCGTGGATGAGTTAGAAGGACAGACTGCAGATGAGGATATTACCAGTGTTCATACTTTTGTCACAGGACTAAAGAAAATGGCCAAAAAGCTTGAGAATGACATACAACTTGTCAACGGTCAGGACCAAGAGACGGGGGAAAAGTTTGCAAAGAATGTGAAAGATGTAGGAGTAGACCCAATGCTGGAATTATCAGAAACTGCTGATTGGCCTCTGGAGTTTGAGAGAAGGCAGAGGGCCATATTTGATCTTTGGCAAACTTGCTATATATCAGTGGTCCATCGAACCTACTTTTTCTTGCTCTTCAAAGGTGATCCAACGGATTCCATTTACATGGAGGTTGAACTTAGGAGATTATCTTTCCTCAAGGAAACTTTTTCCCGTGGAGATCAAGCTGTCGAAAATGGTCAGGCGCTCACACTGGCTTCAAGCATGAAAGCTATCGGTCGGGAGAGAGTAAAGCTAAGCAAGCTGATGCAGAAAAGGTTTTCGGCAGAAGAGAGAAAGAGACTCTTCCAGAAGTGGGGTATTGCACTGCATTCAAAGCGCAGGAGGCTGCAGCTTGCCAACTGCTTGTGGAGTAACACAAAGGATATGAACCACATTACAGATAGCGCCGCCATTGTTGCAAAGCTGGTGATGTTCGCAGAGCAGGGTCAGGCGCTCAAGGGAATGTTCGGGCTTAGTTTCACACCCCCGAAAGCAAGGCGTAGATCCTTTGGATGGAAAAATAGCATGGCATCTCTTATATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003774 GO:0003777 GO:0003824 GO:0005488 GO:0005524 GO:0005575 GO:0005622 GO:0005623 GO:0005856 GO:0005871 GO:0005874 GO:0005875 GO:0005886 GO:0005911 GO:0006928 GO:0007017 GO:0007018 GO:0008144 GO:0008150 GO:0009506 GO:0009987 GO:0015630 GO:0016020 GO:0016462 GO:0016787 GO:0016817 GO:0016818 GO:0016887 GO:0017076 GO:0017111 GO:0030054 GO:0030554 GO:0032553 GO:0032555 GO:0032559 GO:0032991 GO:0035371 GO:0035639 GO:0036094 GO:0043167 GO:0043168 GO:0043226 GO:0043228 GO:0043229 GO:0043232 GO:0044422 GO:0044424 GO:0044430 GO:0044446 GO:0044464 GO:0055044 GO:0071944 GO:0097159 GO:0097367 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:1901265 GO:1901363 GO:1990752

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF3490 Kinesin

Protein Analysis

929

Amino Acids

103.96

Weight (kDa)

5.9

Isoelectric Point (pI)

47.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Microtub_bd	PF16796	24 - 161	5.6e-21	Microtubule binding
Kinesin	PF00225	30 - 346	1.6e-96	Kinesin motor domain
DUF3490	PF11995	751 - 910	4.2e-74	Domain of unknown function (DUF3490)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0003724)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G21300 AT2G21300 AT2G21300 AT2G21300 AT2G21300 AT4G38950 AT4G38950 AT4G38950 AT4G38950 AT4G38950
fragaria_vesca	FvH4_2g10420 FvH4_4g06160
malus_domestica	MD00G1086100.v1.1 MD10G1063300.v1.1
prunus_persica	Prupe.8G084900_v2.0.a1 Prupe.8G084900_v2.0.a1 Prupe.8G084900_v2.0.a1 Prupe.8G084900_v2.0.a1 Prupe.8G084900_v2.0.a1
pyrus_communis	pycom05g04640 pycom10g04890
rosa_chinensis	RchiOBHm_Chr6g0267891
rosa_laevigata	RLG00000013970
rosa_multiflora	Rmu_sc0000434.1_g000005 Rmu_sc0005400.1_g000005
rosa_roxburghii	Rroxscaffold_7G00200350
rosa_rugosa	Rorug06G0034600 Rorug06G0034600 Rorug06G0034600
rosa_samantha	Rh6AG156800 Rh6BG158000 Rh6CG158200 Rh6DG143200
rosa_wichuraiana	Rw6G013620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc16I	TGCGCA	1 cut(s)	411
Acc36I	ACCTGC	2 cut(s)	244, 1661
AccBSI	CCGCTC	1 cut(s)	526
AccI	GTMKAC	3 cut(s)	194, 1467, 2208
AccII	CGCG	1 cut(s)	63
AciI	CCGC	7 cut(s)	184, 500, 526, 1273, 1570, 1956, 2652
AclWI	GGATC	8 cut(s)	512, 1379, 1514, 1527, 1608, 1621, 2342, 2742
AcoI	YGGCCR	2 cut(s)	880, 2115
AcsI	RAATTY	4 cut(s)	742, 1487, 1606, 1861
AcuI	CTGAAG	3 cut(s)	1175, 1293, 1830
AfaI	GTAC	3 cut(s)	353, 405, 1773
AgsI	TTSAA	9 cut(s)	683, 720, 1199, 1417, 1637, 2341, 2378, 2463, 2577
AjnI	CCWGG	3 cut(s)	777, 1135, 1933
Alw21I	GWGCWC	1 cut(s)	591
Alw26I	GTCTC	7 cut(s)	468, 620, 1434, 1753, 1933, 2159, 2536
AlwI	GGATC	8 cut(s)	512, 1379, 1514, 1527, 1608, 1621, 2342, 2742
AlwNI	CAGNNNCTG	1 cut(s)	2234
Ama87I	CYCGRG	1 cut(s)	1457
AoxI	GGCC	8 cut(s)	95, 880, 927, 1077, 1138, 2115, 2243, 2271
ApeKI	GCWGC	8 cut(s)	119, 181, 508, 773, 813, 922, 2590, 2593
ApoI	RAATTY	4 cut(s)	742, 1487, 1606, 1861
AseI	ATTAAT	1 cut(s)	822
Asp700I	GAANNNNTTC	1 cut(s)	2011
AspA2I	CCTAGG	1 cut(s)	703
AspLEI	GCGC	6 cut(s)	412, 1994, 2449, 2583, 2651, 2698
AspS9I	GGNCC	5 cut(s)	1078, 1139, 2158, 2271, 2311
AsuHPI	GGTGA	6 cut(s)	97, 248, 1457, 1875, 2357, 2683
AsuII	TTCGAA	1 cut(s)	1752
AsuNHI	GCTAGC	1 cut(s)	734
AvaI	CYCGRG	1 cut(s)	1457
AvaII	GGWCC	2 cut(s)	2158, 2311
AvrII	CCTAGG	1 cut(s)	703
BalI	TGGCCA	2 cut(s)	882, 2117
BamHI	GGATCC	2 cut(s)	1519, 1613
BanII	GRGCYC	3 cut(s)	274, 591, 974
BauI	CACGAG	1 cut(s)	693
Bbv12I	GWGCWC	1 cut(s)	591
BbvI	GCAGC	8 cut(s)	131, 168, 495, 785, 800, 934, 2577, 2605
BccI	CCATC	7 cut(s)	170, 962, 1198, 1270, 1576, 2322, 2751
BceAI	ACGGC	2 cut(s)	1884, 2021
BciT130I	CCWGG	3 cut(s)	779, 1137, 1935
BcoDI	GTCTC	7 cut(s)	468, 620, 1434, 1753, 1933, 2159, 2536
BfaI	CTAG	9 cut(s)	26, 512, 651, 704, 735, 945, 1110, 1821, 1839
BfmI	CTRYAG	4 cut(s)	920, 1536, 2061, 2591
BfoI	RGCGCY	3 cut(s)	2450, 2652, 2699
BfuAI	ACCTGC	2 cut(s)	244, 1661
BglII	AGATCT	3 cut(s)	22, 1186, 1493
BisI	GCNGC	9 cut(s)	120, 182, 509, 774, 814, 923, 2591, 2594, 2652
BlnI	CCTAGG	1 cut(s)	703
BlpI	GCTNAGC	1 cut(s)	2498
BlsI	GCNGC	9 cut(s)	121, 183, 510, 775, 815, 924, 2592, 2595, 2653
Bme1390I	CCNGG	3 cut(s)	779, 1137, 1935
Bme18I	GGWCC	2 cut(s)	2158, 2311
BmeT110I	CYCGRG	1 cut(s)	1457
BmgT120I	GGNCC	5 cut(s)	1078, 1139, 2158, 2271, 2311
BmiI	GGNNCC	7 cut(s)	271, 532, 575, 1141, 1521, 1615, 1968
BmrFI	CCNGG	3 cut(s)	779, 1137, 1935
BmrI	ACTGGG	1 cut(s)	1688
BmsI	GCATC	7 cut(s)	33, 156, 1413, 1648, 1713, 2499, 2783
BmtI	GCTAGC	1 cut(s)	738
BmuI	ACTGGG	1 cut(s)	1688
BpmI	CTGGAG	1 cut(s)	2270
Bpu10I	CCTNAGC	3 cut(s)	482, 503, 522
Bpu1102I	GCTNAGC	1 cut(s)	2498
Bpu14I	TTCGAA	1 cut(s)	1752
BpuEI	CTTGAG	5 cut(s)	295, 1502, 2147, 2384, 2684
BsaAI	YACGTR	1 cut(s)	1766
BsaI	GGTCTC	2 cut(s)	468, 1933
BsaJI	CCNNGG	8 cut(s)	703, 777, 930, 1458, 1934, 2026, 2037, 2416
BsaWI	WCCGGW	1 cut(s)	1885
BsaXI	ACNNNNNCTCC	4 cut(s)	1109, 1139, 1834, 1864
Bse118I	RCCGGY	1 cut(s)	925
Bse1I	ACTGG	4 cut(s)	575, 1694, 2079, 2460
Bse3DI	GCAATG	1 cut(s)	1241
BseBI	CCWGG	3 cut(s)	779, 1137, 1935
BseDI	CCNNGG	8 cut(s)	703, 777, 930, 1458, 1934, 2026, 2037, 2416
BseGI	GGATG	6 cut(s)	801, 1281, 1348, 1663, 2047, 2762
BseMI	GCAATG	1 cut(s)	1241
BseMII	CTCAG	4 cut(s)	92, 513, 554, 1377
BseNI	ACTGG	4 cut(s)	575, 1694, 2079, 2460
BseRI	GAGGAG	1 cut(s)	1475
BseXI	GCAGC	8 cut(s)	131, 168, 495, 785, 800, 934, 2577, 2605
BseYI	CCCAGC	3 cut(s)	791, 1165, 1337
BsgI	GTGCAG	2 cut(s)	792, 960
Bsh1236I	CGCG	1 cut(s)	63
Bsh1285I	CGRYCG	1 cut(s)	2482
BshFI	GGCC	8 cut(s)	97, 882, 929, 1079, 1140, 2117, 2245, 2273
BsiEI	CGRYCG	1 cut(s)	2482
BsiHKAI	GWGCWC	1 cut(s)	591
BsiHKCI	CYCGRG	1 cut(s)	1457
BsiSI	CCGG	2 cut(s)	926, 1886
BslFI	GGGAC	4 cut(s)	581, 1440, 1546, 1613
BsmAI	GTCTC	7 cut(s)	468, 620, 1434, 1753, 1933, 2159, 2536
BsmBI	CGTCTC	2 cut(s)	1434, 2159
BsmFI	GGGAC	4 cut(s)	581, 1440, 1546, 1613
BsmI	GAATGC	3 cut(s)	149, 1705, 2572
BsnI	GGCC	8 cut(s)	97, 882, 929, 1079, 1140, 2117, 2245, 2273
Bso31I	GGTCTC	2 cut(s)	468, 1933
BsoBI	CYCGRG	1 cut(s)	1457
Bsp119I	TTCGAA	1 cut(s)	1752
Bsp1286I	GDGCHC	3 cut(s)	274, 591, 974
Bsp1407I	TGTACA	2 cut(s)	403, 1771
Bsp1720I	GCTNAGC	1 cut(s)	2498
Bsp68I	TCGCGA	1 cut(s)	63
BspACI	CCGC	7 cut(s)	184, 500, 526, 1273, 1570, 1956, 2652
BspANI	GGCC	8 cut(s)	97, 882, 929, 1079, 1140, 2117, 2245, 2273
BspCNI	CTCAG	4 cut(s)	93, 514, 555, 1376
BspFNI	CGCG	1 cut(s)	63
BspLI	GGNNCC	7 cut(s)	271, 532, 575, 1141, 1521, 1615, 1968
BspMAI	CTGCAG	4 cut(s)	924, 1540, 2065, 2595
BspMI	ACCTGC	2 cut(s)	244, 1661
BspOI	GCTAGC	1 cut(s)	738
BspPI	GGATC	8 cut(s)	512, 1379, 1514, 1527, 1608, 1621, 2342, 2742
BspQI	GCTCTTC	1 cut(s)	2342
BspT104I	TTCGAA	1 cut(s)	1752
BspTNI	GGTCTC	2 cut(s)	468, 1933
BsrBI	CCGCTC	1 cut(s)	526
BsrDI	GCAATG	1 cut(s)	1241
BsrFI	RCCGGY	1 cut(s)	925
BsrGI	TGTACA	2 cut(s)	403, 1771
BsrI	ACTGG	4 cut(s)	575, 1694, 2079, 2460
BssAI	RCCGGY	1 cut(s)	925
BssECI	CCNNGG	8 cut(s)	703, 777, 930, 1458, 1934, 2026, 2037, 2416
BssNAI	GTATAC	1 cut(s)	1468
BssSI	CACGAG	1 cut(s)	693
BssT1I	CCWWGG	3 cut(s)	703, 930, 2026
Bst1107I	GTATAC	1 cut(s)	1468
Bst2BI	CACGAG	1 cut(s)	693
Bst2UI	CCWGG	3 cut(s)	779, 1137, 1935
Bst4CI	ACNGT	5 cut(s)	382, 850, 1445, 1633, 2153
Bst6I	CTCTTC	5 cut(s)	60, 1581, 2342, 2526, 2552
BstAUI	TGTACA	2 cut(s)	403, 1771
BstBAI	YACGTR	1 cut(s)	1766
BstBI	TTCGAA	1 cut(s)	1752
BstDSI	CCRYGG	2 cut(s)	2037, 2416
BstF5I	GGATG	6 cut(s)	801, 1281, 1348, 1663, 2047, 2762
BstFNI	CGCG	1 cut(s)	63
BstH2I	RGCGCY	3 cut(s)	2450, 2652, 2699
BstHHI	GCGC	6 cut(s)	412, 1994, 2449, 2583, 2651, 2698
BstMAI	GTCTC	7 cut(s)	468, 620, 1434, 1753, 1933, 2159, 2536
BstMCI	CGRYCG	1 cut(s)	2482
BstNI	CCWGG	3 cut(s)	779, 1137, 1935
BstNSI	RCATGY	1 cut(s)	410
BstSCI	CCNGG	3 cut(s)	777, 1135, 1933
BstSFI	CTRYAG	4 cut(s)	920, 1536, 2061, 2591
BstUI	CGCG	1 cut(s)	63
BstV1I	GCAGC	8 cut(s)	131, 168, 495, 785, 800, 934, 2577, 2605
BstX2I	RGATCY	6 cut(s)	22, 1186, 1493, 1519, 1613, 2747
BstXI	CCANNNNNNTGG	2 cut(s)	1914, 2368
BstYI	RGATCY	6 cut(s)	22, 1186, 1493, 1519, 1613, 2747
BstZ17I	GTATAC	1 cut(s)	1468
BsuRI	GGCC	8 cut(s)	97, 882, 929, 1079, 1140, 2117, 2245, 2273
BtgI	CCRYGG	2 cut(s)	2037, 2416
BtsCI	GGATG	6 cut(s)	801, 1281, 1348, 1663, 2047, 2762
BtsI	GCAGTG	2 cut(s)	346, 2567
BtsIMutI	CAGTG	8 cut(s)	346, 846, 1629, 1701, 2086, 2313, 2453, 2567
BtuMI	TCGCGA	1 cut(s)	63
BveI	ACCTGC	2 cut(s)	244, 1661
CaiI	CAGNNNCTG	1 cut(s)	2234
CfoI	GCGC	6 cut(s)	412, 1994, 2449, 2583, 2651, 2698
Cfr10I	RCCGGY	1 cut(s)	925
Cfr13I	GGNCC	5 cut(s)	1078, 1139, 2158, 2271, 2311
Csp6I	GTAC	3 cut(s)	352, 404, 1772
CviAII	CATG	8 cut(s)	407, 413, 986, 1066, 1503, 2368, 2467, 2770
CviQI	GTAC	3 cut(s)	352, 404, 1772
DraI	TTTAAA	1 cut(s)	582
EaeI	YGGCCR	2 cut(s)	880, 2115
Eam1104I	CTCTTC	5 cut(s)	60, 1581, 2342, 2526, 2552
EarI	CTCTTC	5 cut(s)	60, 1581, 2342, 2526, 2552
Ecl136II	GAGCTC	1 cut(s)	589
Eco130I	CCWWGG	3 cut(s)	703, 930, 2026
Eco147I	AGGCCT	1 cut(s)	97
Eco24I	GRGCYC	3 cut(s)	274, 591, 974
Eco31I	GGTCTC	2 cut(s)	468, 1933
Eco32I	GATATC	1 cut(s)	1686
Eco47I	GGWCC	2 cut(s)	2158, 2311
Eco53kI	GAGCTC	1 cut(s)	589
Eco57I	CTGAAG	3 cut(s)	1175, 1293, 1830
Eco88I	CYCGRG	1 cut(s)	1457
EcoICRI	GAGCTC	1 cut(s)	589
EcoO109I	RGGNCCY	1 cut(s)	1078
EcoRI	GAATTC	1 cut(s)	1606
EcoRII	CCWGG	3 cut(s)	777, 1135, 1933
EcoRV	GATATC	1 cut(s)	1686
EcoT14I	CCWWGG	3 cut(s)	703, 930, 2026
EcoT22I	ATGCAT	1 cut(s)	149
EcoT38I	GRGCYC	3 cut(s)	274, 591, 974
ErhI	CCWWGG	3 cut(s)	703, 930, 2026
Esp3I	CGTCTC	2 cut(s)	1434, 2159
FaeI	CATG	8 cut(s)	410, 416, 989, 1069, 1506, 2371, 2470, 2773
FalI	AAGNNNNNCTT	2 cut(s)	2531, 2563
FaqI	GGGAC	4 cut(s)	581, 1440, 1546, 1613
FatI	CATG	8 cut(s)	406, 412, 985, 1065, 1502, 2367, 2466, 2769
FauI	CCCGC	3 cut(s)	177, 519, 1949
FauNDI	CATATG	2 cut(s)	325, 1741
FblI	GTMKAC	3 cut(s)	194, 1467, 2208
Fnu4HI	GCNGC	9 cut(s)	120, 182, 509, 774, 814, 923, 2591, 2594, 2652
FokI	GGATG	6 cut(s)	808, 1288, 1355, 1670, 2054, 2769
FriOI	GRGCYC	3 cut(s)	274, 591, 974
Fsp4HI	GCNGC	9 cut(s)	120, 182, 509, 774, 814, 923, 2591, 2594, 2652
FspAI	RTGCGCAY	1 cut(s)	411
FspBI	CTAG	9 cut(s)	26, 512, 651, 704, 735, 945, 1110, 1821, 1839
FspI	TGCGCA	1 cut(s)	411
GlaI	GCGC	6 cut(s)	411, 1993, 2448, 2582, 2650, 2697
GluI	GCNGC	9 cut(s)	120, 182, 509, 774, 814, 923, 2591, 2594, 2652
GsaI	CCCAGC	3 cut(s)	795, 1169, 1341
GsuI	CTGGAG	1 cut(s)	2270
HaeII	RGCGCY	3 cut(s)	2450, 2652, 2699
HaeIII	GGCC	8 cut(s)	97, 882, 929, 1079, 1140, 2117, 2245, 2273
HapII	CCGG	2 cut(s)	926, 1886
HhaI	GCGC	6 cut(s)	412, 1994, 2449, 2583, 2651, 2698
Hin1II	CATG	8 cut(s)	410, 416, 989, 1069, 1506, 2371, 2470, 2773
Hin6I	GCGC	6 cut(s)	410, 1992, 2447, 2581, 2649, 2696
HinP1I	GCGC	6 cut(s)	410, 1992, 2447, 2581, 2649, 2696
HincII	GTYRAC	2 cut(s)	1836, 2149
HindII	GTYRAC	2 cut(s)	1836, 2149
HindIII	AAGCTT	2 cut(s)	1927, 2123
HpaI	GTTAAC	1 cut(s)	1836
HpaII	CCGG	2 cut(s)	926, 1886
HphI	GGTGA	6 cut(s)	97, 248, 1457, 1875, 2357, 2683
Hpy166II	GTNNAC	9 cut(s)	195, 354, 378, 751, 833, 1468, 1836, 2149, 2209
Hpy8I	GTNNAC	9 cut(s)	195, 354, 378, 751, 833, 1468, 1836, 2149, 2209
HpyCH4III	ACNGT	5 cut(s)	382, 850, 1445, 1633, 2153
HpyCH4IV	ACGT	3 cut(s)	350, 1748, 1765
HpySE526I	ACGT	3 cut(s)	350, 1748, 1765
Hsp92II	CATG	8 cut(s)	410, 416, 989, 1069, 1506, 2371, 2470, 2773
HspAI	GCGC	6 cut(s)	410, 1992, 2447, 2581, 2649, 2696
KroI	GCCGGC	1 cut(s)	925
KroNI	GCCGGC	1 cut(s)	927
KspAI	GTTAAC	1 cut(s)	1836
LguI	GCTCTTC	1 cut(s)	2342
LmnI	GCTCC	4 cut(s)	50, 269, 586, 594
Lsp1109I	GCAGC	8 cut(s)	131, 168, 495, 785, 800, 934, 2577, 2605
LweI	GCATC	7 cut(s)	33, 156, 1413, 1648, 1713, 2499, 2783
MaeI	CTAG	9 cut(s)	26, 512, 651, 704, 735, 945, 1110, 1821, 1839
MaeII	ACGT	3 cut(s)	350, 1748, 1765
MaeIII	GTNAC	4 cut(s)	1036, 1645, 2095, 2615
MbiI	CCGCTC	1 cut(s)	526
MfeI	CAATTG	3 cut(s)	9, 678, 1775
MflI	RGATCY	6 cut(s)	22, 1186, 1493, 1519, 1613, 2747
MhlI	GDGCHC	3 cut(s)	274, 591, 974
MlsI	TGGCCA	2 cut(s)	882, 2117
MluNI	TGGCCA	2 cut(s)	882, 2117
MlyI	GAGTC	3 cut(s)	1598, 1726, 2538
MmeI	TCCRAC	5 cut(s)	227, 1261, 1826, 1899, 2375
Mox20I	TGGCCA	2 cut(s)	882, 2117
Mph1103I	ATGCAT	1 cut(s)	149
MroNI	GCCGGC	1 cut(s)	925
MroXI	GAANNNNTTC	1 cut(s)	2011
MscI	TGGCCA	2 cut(s)	882, 2117
MseI	TTAA	8 cut(s)	435, 581, 635, 669, 803, 822, 1086, 1835
MslI	CAYNNNNRTG	3 cut(s)	411, 1431, 2366
Msp20I	TGGCCA	2 cut(s)	882, 2117
MspA1I	CMGCKG	2 cut(s)	791, 1572
MspI	CCGG	2 cut(s)	926, 1886
MspR9I	CCNGG	3 cut(s)	779, 1137, 1935
MunI	CAATTG	3 cut(s)	9, 678, 1775
Mva1269I	GAATGC	3 cut(s)	149, 1705, 2572
MvaI	CCWGG	3 cut(s)	779, 1137, 1935
MvnI	CGCG	1 cut(s)	63
NaeI	GCCGGC	1 cut(s)	927
NdeI	CATATG	2 cut(s)	325, 1741
NgoMIV	GCCGGC	1 cut(s)	925
NheI	GCTAGC	1 cut(s)	734
NlaIII	CATG	8 cut(s)	410, 416, 989, 1069, 1506, 2371, 2470, 2773
NlaIV	GGNNCC	7 cut(s)	271, 532, 575, 1141, 1521, 1615, 1968
NmuCI	GTSAC	2 cut(s)	1036, 2095
NruI	TCGCGA	1 cut(s)	63
NsbI	TGCGCA	1 cut(s)	411
NsiI	ATGCAT	1 cut(s)	149
NspI	RCATGY	1 cut(s)	410
NspV	TTCGAA	1 cut(s)	1752
PceI	AGGCCT	1 cut(s)	97
PciSI	GCTCTTC	1 cut(s)	2342
PctI	GAATGC	3 cut(s)	149, 1705, 2572
PdiI	GCCGGC	1 cut(s)	927
PdmI	GAANNNNTTC	1 cut(s)	2011
PkrI	GCNGC	9 cut(s)	121, 183, 510, 775, 815, 924, 2592, 2595, 2653
PleI	GAGTC	3 cut(s)	1597, 1725, 2538
PpsI	GAGTC	3 cut(s)	1597, 1725, 2538
Ppu21I	YACGTR	1 cut(s)	1766
PshBI	ATTAAT	1 cut(s)	822
Psp124BI	GAGCTC	1 cut(s)	591
Psp6I	CCWGG	3 cut(s)	777, 1135, 1933
PspFI	CCCAGC	3 cut(s)	791, 1165, 1337
PspGI	CCWGG	3 cut(s)	777, 1135, 1933
PspN4I	GGNNCC	7 cut(s)	271, 532, 575, 1141, 1521, 1615, 1968
PspPI	GGNCC	5 cut(s)	1078, 1139, 2158, 2271, 2311
PsrI	GAACNNNNNNTAC	2 cut(s)	1746, 1778
PstI	CTGCAG	4 cut(s)	924, 1540, 2065, 2595
PstNI	CAGNNNCTG	1 cut(s)	2234
PsuI	RGATCY	6 cut(s)	22, 1186, 1493, 1519, 1613, 2747
PvuII	CAGCTG	1 cut(s)	791
RruI	TCGCGA	1 cut(s)	63
RsaI	GTAC	3 cut(s)	353, 405, 1773
RsaNI	GTAC	3 cut(s)	352, 404, 1772
RseI	CAYNNNNRTG	3 cut(s)	411, 1431, 2366
SacI	GAGCTC	1 cut(s)	591
SapI	GCTCTTC	1 cut(s)	2342
SaqAI	TTAA	8 cut(s)	435, 581, 635, 669, 803, 822, 1086, 1835
SatI	GCNGC	9 cut(s)	120, 182, 509, 774, 814, 923, 2591, 2594, 2652
Sau96I	GGNCC	5 cut(s)	1078, 1139, 2158, 2271, 2311
SchI	GAGTC	3 cut(s)	1598, 1726, 2538
ScrFI	CCNGG	3 cut(s)	779, 1137, 1935
SduI	GDGCHC	3 cut(s)	274, 591, 974
SfaNI	GCATC	7 cut(s)	33, 156, 1413, 1648, 1713, 2499, 2783
SfcI	CTRYAG	4 cut(s)	920, 1536, 2061, 2591
SfuI	TTCGAA	1 cut(s)	1752
SinI	GGWCC	2 cut(s)	2158, 2311
SmiMI	CAYNNNNRTG	3 cut(s)	411, 1431, 2366
SmlI	CTYRAG	5 cut(s)	310, 1481, 2126, 2399, 2699
SmoI	CTYRAG	5 cut(s)	310, 1481, 2126, 2399, 2699
SseBI	AGGCCT	1 cut(s)	97
SsiI	CCGC	7 cut(s)	184, 500, 526, 1273, 1570, 1956, 2652
SspI	AATATT	1 cut(s)	393
SspMI	CTAG	9 cut(s)	26, 512, 651, 704, 735, 945, 1110, 1821, 1839
SstI	GAGCTC	1 cut(s)	591
StuI	AGGCCT	1 cut(s)	97
StyD4I	CCNGG	3 cut(s)	777, 1135, 1933
StyI	CCWWGG	3 cut(s)	703, 930, 2026
TaaI	ACNGT	5 cut(s)	382, 850, 1445, 1633, 2153
TaiI	ACGT	3 cut(s)	353, 1751, 1768
TaqI	TCGA	3 cut(s)	1752, 2317, 2433
TaqII	GACCGA	1 cut(s)	2468
TatI	WGTACW	2 cut(s)	403, 1771
TauI	GCSGC	1 cut(s)	2654
Tru1I	TTAA	8 cut(s)	435, 581, 635, 669, 803, 822, 1086, 1835
Tru9I	TTAA	8 cut(s)	435, 581, 635, 669, 803, 822, 1086, 1835
TscAI	CASTG	8 cut(s)	346, 853, 1636, 1701, 2086, 2313, 2460, 2574
TseFI	GTSAC	2 cut(s)	1036, 2095
TseI	GCWGC	8 cut(s)	119, 181, 508, 773, 813, 922, 2590, 2593
Tsp45I	GTSAC	2 cut(s)	1036, 2095
TspGWI	ACGGA	2 cut(s)	1485, 2369
TspRI	CASTG	8 cut(s)	346, 853, 1636, 1701, 2086, 2313, 2460, 2574
VpaK11BI	GGWCC	2 cut(s)	2158, 2311
VspI	ATTAAT	1 cut(s)	822
XapI	RAATTY	4 cut(s)	742, 1487, 1606, 1861
XbaI	TCTAGA	2 cut(s)	650, 1820
XceI	RCATGY	1 cut(s)	410
XmaJI	CCTAGG	1 cut(s)	703
XmiI	GTMKAC	3 cut(s)	194, 1467, 2208
XmnI	GAANNNNTTC	1 cut(s)	2011
XspI	CTAG	9 cut(s)	26, 512, 651, 704, 735, 945, 1110, 1821, 1839
Zsp2I	ATGCAT	1 cut(s)	149

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.