Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0006730

Physical Location & Seq

Genomic Coordinates Forward (+)

24120559 .. 24123938

Gene Structure 3380 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1113700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1746 bp

ATGTACTCTGGGAGACCATCTGGTGAAGAATCTCTTCATCAAGATGTTGAAGCTTTGAGTGTGTCAAAGCGTCTCATGAGAAGTGTTAGCCAGAAGTTGAGGAAGAAGAATCAGAGATCTGTAGGAGATGACGATGATGATGTGAGGGGAATATCTTTGAGATGTCTTACTCTGTATGGTAGGGGTGGAGGTTGCAGAGTAGGTGCTGACACAGGTGATGATTTTGGGGATACCAGTAACACGAGGAGGCCTTCCAGTGCCAGCGATGATGGCAAAGGATACAGACCAATATGTGGTACCGAGAAATCTGGAGTTGATTGCTTCTTATATGGGGTGAGGGAGAGATTTTTGAAGAAACATAACAGAAAGGATTTTGAGCTTGAAGAATCAATAAGAAATAGCAGGATGCACATTTTTCTTCCAGATGACATACTGGAAATGTGCTTGGTGAGGCTCCCACTAACCAGTCTCATGACTGCACGCCTCGTGTGCAAGAAATGGAAACACTTGACTACTACTACTCGTTTCCTGCAGATGAGACGGGAAGGCTCACATCAAAACCCATGGTTGTTTCTTTTTGGTGCTGTTAAAGATGGTTATTGCTCTGGGGAGATACATGCATTGGATGTGTCTCTAAACCAATGGCATGGTGTAGATGCTGGCATTCTAAAGGGAAGGTTCATGTTTTCTGTTGCTAGTGTCCAGGATGATATTTACGTTGTTGGAGGTTGTTCTAGCTTGAACAACTTTGGGAGGATGGATAAGAGCTCATTTAAGACACACAAAGGGGTGATACTGTTTAGTCCCCTAACAAAATCTTGGCGCAATGTTGCTCCAATGAAGTATGCAAGATCAATGCCTATTTTAGGAGTTTCGGAGGTAACTTCAGATTTTTCCACCAGTCAAAGTTATCAAAGTCGCCAAGATAGACGTATTCCCAGATCACGGGTAGGTGGAGTGTCAGAGGTTTATGAAGATCCTCACAGGCTTTCTCTTAGGCGTCAACACGGATGTTCTGGTGACGACAATGAGTCTTCGGTGTTGCCCAATAGAAAGTCATATAAATTTTTGAAACAAAAAAGTGATCACTCATCCATGAAGGGTTGTAGAAGGGTTGTGCTCATTGCTGTAGGTGGTCTTGGATCCTGGGATGAACCTTTGGATTCTGGAGAAATATATGATTCTGTTTCAAAGAAATGGACTGAAATTCAAGGGTTGCCTGTGGACTTTGGGTTTGCTTGTTCTGGGGTTATTTGTAATGGGATGTTTTATGTTTATTCTGAAACTGACAAGCTCGCAGGATATGACATAGAAAGGGGTTTCTGGATTGGAATCCAAACCACTCCTTTCCCACCCCGCGTTCATGAATATTACCCACAACTGGTATCTTGTAATGGCCGGCTCTTCATGCTTTCTGTCTTCTGGTGTGAAGGGGATGGTCAGATAGGCCGGAGAAACAAGGCCGTTAGAAAAGTATGGGAGCTGGATCTCATGTACCATAACTGGACCGAGGTCTCAGTACATCCTGATGCTCCAATGGACTGGAATGCTGCGTTTGTGGCAGACGGAAACGTGATATTTGGTATTGAGATGTTCAAAATATTTGGGCAAGTATTGGATTTTTTTACTGCATGTGACGTTTCTGATATGGGGATGAAGTGGAGTCATGTCTCAAGGAACTTTGTAACTCGCGAGCTGGATGCTTCTTCGTGCTTGTCCAAGTCAATGGCTGTGCTACATCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0001558 GO:0005575 GO:0005622 GO:0005623 GO:0008150 GO:0008361 GO:0009987 GO:0016043 GO:0019005 GO:0031461 GO:0031463 GO:0032535 GO:0032991 GO:0040008 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051128 GO:0065007 GO:0065008 GO:0071840 GO:0090066 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box Kelch_1 Kelch_6

Protein Analysis

582

Amino Acids

65.6

Weight (kDa)

8.65

Isoelectric Point (pI)

44.95

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	140 - 176	1.3e-08	F-box domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	157 - 243	2.5e-06	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1	PF01344	226 - 281	5.5e-06	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006730)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42350 AT5G42360
fragaria_vesca	FvH4_5g25640 FvH4_5g25640 FvH4_5g25640 FvH4_5g25640 FvH4_5g25640
malus_domestica	MD00G1113700.v1.1 MD06G1007100.v1.1
prunus_persica	Prupe.5G009000_v2.0.a1 Prupe.5G009000_v2.0.a1 Prupe.5G009000_v2.0.a1 Prupe.5G009000_v2.0.a1
pyrus_communis	pycom06g00620
rosa_chinensis	RchiOBHm_Chr7g0215931
rosa_laevigata	RLG00000002606
rosa_multiflora	Rmu_sc0006240.1_g000003
rosa_roxburghii	Rroxscaffold_3G00243790
rosa_rugosa	Rorug07G0156900 Rorug07G0157000 Rorug07G0157100
rosa_samantha	Rh7AG295000 Rh7BG287000 Rh7CG315100 Rh7DG297800
rosa_wichuraiana	Rw7G025060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	296
AccB1I	GGYRCC	1 cut(s)	296
AccB7I	CCANNNNNTGG	1 cut(s)	293
AccII	CGCG	2 cut(s)	1359, 1692
AciI	CCGC	1 cut(s)	1357
AclWI	GGATC	4 cut(s)	971, 1137, 1150, 1494
AcoI	YGGCCR	1 cut(s)	1396
AcsI	RAATTY	2 cut(s)	1064, 1206
AcuI	CTGAAG	1 cut(s)	870
AcyI	GRCGYC	1 cut(s)	1000
AfaI	GTAC	4 cut(s)	5, 298, 1496, 1521
AfiI	CCNNNNNNNGG	4 cut(s)	293, 866, 945, 1381
AgsI	TTSAA	8 cut(s)	50, 352, 383, 742, 1072, 1191, 1211, 1597
AhdI	GACNNNNNGTC	1 cut(s)	1030
AjnI	CCWGG	2 cut(s)	702, 1145
AluBI	AGCT	7 cut(s)	53, 379, 738, 768, 1294, 1483, 1696
AluI	AGCT	7 cut(s)	53, 379, 738, 768, 1294, 1483, 1696
Alw21I	GWGCWC	2 cut(s)	770, 1122
Alw26I	GTCTC	7 cut(s)	7, 77, 473, 532, 636, 1519, 1675
AlwI	GGATC	4 cut(s)	971, 1137, 1150, 1494
AoxI	GGCC	4 cut(s)	248, 1396, 1447, 1461
ApeKI	GCWGC	1 cut(s)	1550
ApoI	RAATTY	2 cut(s)	1064, 1206
ArsI	GACNNNNNNTTYG	2 cut(s)	1217, 1249
Asp700I	GAANNNNTTC	1 cut(s)	33
Asp718I	GGTACC	1 cut(s)	296
AspLEI	GCGC	1 cut(s)	825
AspS9I	GGNCC	1 cut(s)	1506
AsuHPI	GGTGA	6 cut(s)	35, 227, 346, 460, 802, 1031
AvaII	GGWCC	1 cut(s)	1506
BamHI	GGATCC	1 cut(s)	1142
BanI	GGYRCC	1 cut(s)	296
BanII	GRGCYC	1 cut(s)	770
BauI	CACGAG	2 cut(s)	241, 485
BbsI	GAAGAC	2 cut(s)	1026, 1411
Bbv12I	GWGCWC	2 cut(s)	770, 1122
BbvI	GCAGC	1 cut(s)	1537
BccI	CCATC	5 cut(s)	25, 263, 587, 751, 1430
BceAI	ACGGC	1 cut(s)	1448
BcgI	CGANNNNNNTGC	2 cut(s)	1682, 1716
BciT130I	CCWGG	2 cut(s)	704, 1147
BciVI	GTATCC	2 cut(s)	223, 272
BclI	TGATCA	1 cut(s)	1084
BcoDI	GTCTC	7 cut(s)	7, 77, 473, 532, 636, 1519, 1675
BfaI	CTAG	2 cut(s)	696, 735
BfmI	CTRYAG	3 cut(s)	120, 530, 1128
BfuI	GTATCC	2 cut(s)	223, 272
BglII	AGATCT	1 cut(s)	116
BisI	GCNGC	1 cut(s)	1551
BlsI	GCNGC	1 cut(s)	1552
Bme1390I	CCNGG	2 cut(s)	704, 1147
Bme18I	GGWCC	1 cut(s)	1506
BmeRI	GACNNNNNGTC	1 cut(s)	1030
BmgT120I	GGNCC	1 cut(s)	1506
BmiI	GGNNCC	3 cut(s)	298, 455, 1144
BmrFI	CCNGG	2 cut(s)	704, 1147
BmsI	GCATC	4 cut(s)	396, 646, 1519, 1690
BoxI	GACNNNNGTC	1 cut(s)	1511
BpiI	GAAGAC	2 cut(s)	1026, 1411
BpmI	CTGGAG	2 cut(s)	330, 1188
BpuEI	CTTGAG	1 cut(s)	1657
BsaBI	GATNNNNATC	1 cut(s)	1331
BsaHI	GRCGYC	1 cut(s)	1000
BsaI	GGTCTC	2 cut(s)	7, 1519
BsaJI	CCNNGG	3 cut(s)	563, 1146, 1509
Bsc4I	CCNNNNNNNGG	4 cut(s)	293, 866, 945, 1381
Bse118I	RCCGGY	1 cut(s)	1398
Bse1I	ACTGG	8 cut(s)	234, 255, 438, 465, 900, 1386, 1508, 1547
Bse3DI	GCAATG	2 cut(s)	832, 1122
Bse8I	GATNNNNATC	1 cut(s)	1331
BseBI	CCWGG	2 cut(s)	704, 1147
BseDI	CCNNGG	3 cut(s)	563, 1146, 1509
BseJI	GATNNNNATC	1 cut(s)	1331
BseLI	CCNNNNNNNGG	4 cut(s)	293, 866, 945, 1381
BseMI	GCAATG	2 cut(s)	832, 1122
BseMII	CTCAG	1 cut(s)	1530
BseNI	ACTGG	8 cut(s)	234, 255, 438, 465, 900, 1386, 1508, 1547
BseRI	GAGGAG	1 cut(s)	259
BseXI	GCAGC	1 cut(s)	1537
BsgI	GTGCAG	1 cut(s)	462
Bsh1236I	CGCG	2 cut(s)	1359, 1692
BshFI	GGCC	4 cut(s)	250, 1398, 1449, 1463
BshNI	GGYRCC	1 cut(s)	296
BsiHKAI	GWGCWC	2 cut(s)	770, 1122
BsiSI	CCGG	2 cut(s)	1399, 1450
BslFI	GGGAC	1 cut(s)	789
BslI	CCNNNNNNNGG	4 cut(s)	293, 866, 945, 1381
BsmAI	GTCTC	7 cut(s)	7, 77, 473, 532, 636, 1519, 1675
BsmBI	CGTCTC	2 cut(s)	77, 532
BsmFI	GGGAC	1 cut(s)	789
BsmI	GAATGC	2 cut(s)	663, 1552
BsnI	GGCC	4 cut(s)	250, 1398, 1449, 1463
Bso31I	GGTCTC	2 cut(s)	7, 1519
Bsp1286I	GDGCHC	2 cut(s)	770, 1122
Bsp143I	GATC	7 cut(s)	116, 851, 941, 976, 1084, 1142, 1486
Bsp19I	CCATGG	1 cut(s)	563
Bsp68I	TCGCGA	1 cut(s)	1692
BspACI	CCGC	1 cut(s)	1357
BspANI	GGCC	4 cut(s)	250, 1398, 1449, 1463
BspCNI	CTCAG	1 cut(s)	1529
BspFNI	CGCG	2 cut(s)	1359, 1692
BspHI	TCATGA	3 cut(s)	75, 471, 1363
BspLI	GGNNCC	3 cut(s)	298, 455, 1144
BspMAI	CTGCAG	1 cut(s)	534
BspPI	GGATC	4 cut(s)	971, 1137, 1150, 1494
BspQI	GCTCTTC	1 cut(s)	1409
BspT107I	GGYRCC	1 cut(s)	296
BspTNI	GGTCTC	2 cut(s)	7, 1519
BsrDI	GCAATG	2 cut(s)	832, 1122
BsrFI	RCCGGY	1 cut(s)	1398
BsrI	ACTGG	8 cut(s)	234, 255, 438, 465, 900, 1386, 1508, 1547
BssAI	RCCGGY	1 cut(s)	1398
BssECI	CCNNGG	3 cut(s)	563, 1146, 1509
BssMI	GATC	7 cut(s)	116, 851, 941, 976, 1084, 1142, 1486
BssNI	GRCGYC	1 cut(s)	1000
BssSI	CACGAG	2 cut(s)	241, 485
BssT1I	CCWWGG	1 cut(s)	563
Bst2BI	CACGAG	2 cut(s)	241, 485
Bst2UI	CCWGG	2 cut(s)	704, 1147
Bst4CI	ACNGT	1 cut(s)	798
Bst6I	CTCTTC	2 cut(s)	39, 1409
BstACI	GRCGYC	1 cut(s)	1000
BstC8I	GCNNGC	6 cut(s)	262, 481, 661, 1296, 1400, 1694
BstDEI	CTNAG	2 cut(s)	995, 1516
BstDSI	CCRYGG	1 cut(s)	563
BstENI	CCTNNNNNAGG	1 cut(s)	864
BstFNI	CGCG	2 cut(s)	1359, 1692
BstHHI	GCGC	1 cut(s)	825
BstKTI	GATC	7 cut(s)	119, 854, 944, 979, 1087, 1145, 1489
BstMAI	GTCTC	7 cut(s)	7, 77, 473, 532, 636, 1519, 1675
BstMBI	GATC	7 cut(s)	116, 851, 941, 976, 1084, 1142, 1486
BstMWI	GCNNNNNNNGC	4 cut(s)	270, 489, 1408, 1559
BstNI	CCWGG	2 cut(s)	704, 1147
BstNSI	RCATGY	2 cut(s)	620, 1635
BstPAI	GACNNNNGTC	1 cut(s)	1511
BstSCI	CCNGG	2 cut(s)	702, 1145
BstSFI	CTRYAG	3 cut(s)	120, 530, 1128
BstUI	CGCG	2 cut(s)	1359, 1692
BstV1I	GCAGC	1 cut(s)	1537
BstV2I	GAAGAC	2 cut(s)	1026, 1411
BstX2I	RGATCY	4 cut(s)	116, 976, 1142, 1486
BstXI	CCANNNNNNTGG	3 cut(s)	647, 1542, 1726
BstYI	RGATCY	4 cut(s)	116, 976, 1142, 1486
BsuI	GTATCC	2 cut(s)	223, 272
BsuRI	GGCC	4 cut(s)	250, 1398, 1449, 1463
BtgI	CCRYGG	1 cut(s)	563
BtgZI	GCGATG	1 cut(s)	279
BtsIMutI	CAGTG	1 cut(s)	262
BtuMI	TCGCGA	1 cut(s)	1692
Cac8I	GCNNGC	6 cut(s)	262, 481, 661, 1296, 1400, 1694
CciI	TCATGA	3 cut(s)	75, 471, 1363
CfoI	GCGC	1 cut(s)	825
Cfr10I	RCCGGY	1 cut(s)	1398
Cfr13I	GGNCC	1 cut(s)	1506
CseI	GACGC	2 cut(s)	59, 989
Csp6I	GTAC	4 cut(s)	4, 297, 1495, 1520
CviQI	GTAC	4 cut(s)	4, 297, 1495, 1520
DdeI	CTNAG	2 cut(s)	995, 1516
DpnI	GATC	7 cut(s)	118, 853, 943, 978, 1086, 1144, 1488
DpnII	GATC	7 cut(s)	116, 851, 941, 976, 1084, 1142, 1486
DriI	GACNNNNNGTC	1 cut(s)	1030
EaeI	YGGCCR	1 cut(s)	1396
Eam1104I	CTCTTC	2 cut(s)	39, 1409
Eam1105I	GACNNNNNGTC	1 cut(s)	1030
EarI	CTCTTC	2 cut(s)	39, 1409
Ecl136II	GAGCTC	1 cut(s)	768
Eco130I	CCWWGG	1 cut(s)	563
Eco147I	AGGCCT	1 cut(s)	250
Eco24I	GRGCYC	1 cut(s)	770
Eco31I	GGTCTC	2 cut(s)	7, 1519
Eco47I	GGWCC	1 cut(s)	1506
Eco53kI	GAGCTC	1 cut(s)	768
Eco57I	CTGAAG	1 cut(s)	870
EcoICRI	GAGCTC	1 cut(s)	768
EcoNI	CCTNNNNNAGG	1 cut(s)	864
EcoRII	CCWGG	2 cut(s)	702, 1145
EcoT14I	CCWWGG	1 cut(s)	563
EcoT22I	ATGCAT	1 cut(s)	622
EcoT38I	GRGCYC	1 cut(s)	770
ErhI	CCWWGG	1 cut(s)	563
Esp3I	CGTCTC	2 cut(s)	77, 532
FalI	AAGNNNNNCTT	2 cut(s)	18, 50
FaqI	GGGAC	1 cut(s)	789
FauI	CCCGC	1 cut(s)	1364
FbaI	TGATCA	1 cut(s)	1084
Fnu4HI	GCNGC	1 cut(s)	1551
FriOI	GRGCYC	1 cut(s)	770
Fsp4HI	GCNGC	1 cut(s)	1551
FspBI	CTAG	2 cut(s)	696, 735
GlaI	GCGC	1 cut(s)	824
GluI	GCNGC	1 cut(s)	1551
GsuI	CTGGAG	2 cut(s)	330, 1188
HaeIII	GGCC	4 cut(s)	250, 1398, 1449, 1463
HapII	CCGG	2 cut(s)	1399, 1450
HgaI	GACGC	2 cut(s)	59, 989
HhaI	GCGC	1 cut(s)	825
Hin1I	GRCGYC	1 cut(s)	1000
Hin6I	GCGC	1 cut(s)	823
HinP1I	GCGC	1 cut(s)	823
HincII	GTYRAC	1 cut(s)	1004
HindII	GTYRAC	1 cut(s)	1004
HindIII	AAGCTT	1 cut(s)	51
HinfI	GANTC	8 cut(s)	29, 109, 386, 1031, 1163, 1181, 1332, 1663
HpaII	CCGG	2 cut(s)	1399, 1450
HphI	GGTGA	6 cut(s)	35, 227, 346, 460, 802, 1031
Hpy166II	GTNNAC	2 cut(s)	1004, 1225
Hpy188I	TCNGA	7 cut(s)	114, 877, 889, 964, 1282, 1443, 1645
Hpy8I	GTNNAC	2 cut(s)	1004, 1225
HpyAV	CCTTC	6 cut(s)	261, 539, 669, 1093, 1104, 1424
HpyCH4III	ACNGT	1 cut(s)	798
HpyCH4IV	ACGT	4 cut(s)	717, 931, 1572, 1638
HpyCH4V	TGCA	8 cut(s)	195, 409, 479, 492, 532, 620, 848, 1631
HpyF10VI	GCNNNNNNNGC	4 cut(s)	270, 489, 1408, 1559
HpyF3I	CTNAG	2 cut(s)	995, 1516
HpySE526I	ACGT	4 cut(s)	717, 931, 1572, 1638
Hsp92I	GRCGYC	1 cut(s)	1000
HspAI	GCGC	1 cut(s)	823
KpnI	GGTACC	1 cut(s)	300
KroI	GCCGGC	1 cut(s)	1398
KroNI	GCCGGC	1 cut(s)	1400
Ksp22I	TGATCA	1 cut(s)	1084
Kzo9I	GATC	7 cut(s)	116, 851, 941, 976, 1084, 1142, 1486
LguI	GCTCTTC	1 cut(s)	1409
LmnI	GCTCC	4 cut(s)	459, 838, 1480, 1537
Lsp1109I	GCAGC	1 cut(s)	1537
LweI	GCATC	4 cut(s)	396, 646, 1519, 1690
MaeI	CTAG	2 cut(s)	696, 735
MaeII	ACGT	4 cut(s)	717, 931, 1572, 1638
MaeIII	GTNAC	5 cut(s)	236, 880, 1019, 1634, 1684
MalI	GATC	7 cut(s)	118, 853, 943, 978, 1086, 1144, 1488
MboI	GATC	7 cut(s)	116, 851, 941, 976, 1084, 1142, 1486
MflI	RGATCY	4 cut(s)	116, 976, 1142, 1486
MhlI	GDGCHC	2 cut(s)	770, 1122
MluCI	AATT	2 cut(s)	1064, 1206
MlyI	GAGTC	2 cut(s)	1040, 1672
MmeI	TCCRAC	1 cut(s)	703
Mph1103I	ATGCAT	1 cut(s)	622
MroNI	GCCGGC	1 cut(s)	1398
MroXI	GAANNNNTTC	1 cut(s)	33
MseI	TTAA	2 cut(s)	588, 774
MslI	CAYNNNNRTG	2 cut(s)	42, 1527
MspI	CCGG	2 cut(s)	1399, 1450
MspR9I	CCNGG	2 cut(s)	704, 1147
Mva1269I	GAATGC	2 cut(s)	663, 1552
MvaI	CCWGG	2 cut(s)	704, 1147
MvnI	CGCG	2 cut(s)	1359, 1692
MwoI	GCNNNNNNNGC	4 cut(s)	270, 489, 1408, 1559
NaeI	GCCGGC	1 cut(s)	1400
NcoI	CCATGG	1 cut(s)	563
NdeII	GATC	7 cut(s)	116, 851, 941, 976, 1084, 1142, 1486
NgoMIV	GCCGGC	1 cut(s)	1398
NlaIV	GGNNCC	3 cut(s)	298, 455, 1144
NmuCI	GTSAC	2 cut(s)	1019, 1634
NruI	TCGCGA	1 cut(s)	1692
NsiI	ATGCAT	1 cut(s)	622
NspI	RCATGY	2 cut(s)	620, 1635
PagI	TCATGA	3 cut(s)	75, 471, 1363
PceI	AGGCCT	1 cut(s)	250
PciSI	GCTCTTC	1 cut(s)	1409
PctI	GAATGC	2 cut(s)	663, 1552
PdiI	GCCGGC	1 cut(s)	1400
PdmI	GAANNNNTTC	1 cut(s)	33
PfeI	GAWTC	6 cut(s)	29, 109, 386, 1163, 1181, 1332
PflMI	CCANNNNNTGG	1 cut(s)	293
PfoI	TCCNGGA	1 cut(s)	702
PkrI	GCNGC	1 cut(s)	1552
PleI	GAGTC	2 cut(s)	1039, 1671
PpsI	GAGTC	2 cut(s)	1039, 1671
PshAI	GACNNNNGTC	1 cut(s)	1511
Psp124BI	GAGCTC	1 cut(s)	770
Psp6I	CCWGG	2 cut(s)	702, 1145
PspGI	CCWGG	2 cut(s)	702, 1145
PspN4I	GGNNCC	3 cut(s)	298, 455, 1144
PspPI	GGNCC	1 cut(s)	1506
PstI	CTGCAG	1 cut(s)	534
PsuI	RGATCY	4 cut(s)	116, 976, 1142, 1486
RruI	TCGCGA	1 cut(s)	1692
RsaI	GTAC	4 cut(s)	5, 298, 1496, 1521
RsaNI	GTAC	4 cut(s)	4, 297, 1495, 1520
RseI	CAYNNNNRTG	2 cut(s)	42, 1527
SacI	GAGCTC	1 cut(s)	770
SapI	GCTCTTC	1 cut(s)	1409
SaqAI	TTAA	2 cut(s)	588, 774
SatI	GCNGC	1 cut(s)	1551
Sau3AI	GATC	7 cut(s)	116, 851, 941, 976, 1084, 1142, 1486
Sau96I	GGNCC	1 cut(s)	1506
SchI	GAGTC	2 cut(s)	1040, 1672
ScrFI	CCNGG	2 cut(s)	704, 1147
SduI	GDGCHC	2 cut(s)	770, 1122
SfaNI	GCATC	4 cut(s)	396, 646, 1519, 1690
SfcI	CTRYAG	3 cut(s)	120, 530, 1128
SinI	GGWCC	1 cut(s)	1506
SmiMI	CAYNNNNRTG	2 cut(s)	42, 1527
SmlI	CTYRAG	1 cut(s)	1672
SmoI	CTYRAG	1 cut(s)	1672
Sse9I	AATT	2 cut(s)	1064, 1206
SseBI	AGGCCT	1 cut(s)	250
SsiI	CCGC	1 cut(s)	1357
SspI	AATATT	2 cut(s)	1370, 1602
SspMI	CTAG	2 cut(s)	696, 735
SstI	GAGCTC	1 cut(s)	770
StuI	AGGCCT	1 cut(s)	250
StyD4I	CCNGG	2 cut(s)	702, 1145
StyI	CCWWGG	1 cut(s)	563
TaaI	ACNGT	1 cut(s)	798
TaiI	ACGT	4 cut(s)	720, 934, 1575, 1641
TaqII	GACCGA	1 cut(s)	1523
TasI	AATT	2 cut(s)	1064, 1206
TatI	WGTACW	2 cut(s)	3, 1519
TfiI	GAWTC	6 cut(s)	29, 109, 386, 1163, 1181, 1332
Tru1I	TTAA	2 cut(s)	588, 774
Tru9I	TTAA	2 cut(s)	588, 774
TscAI	CASTG	1 cut(s)	262
TseFI	GTSAC	2 cut(s)	1019, 1634
TseI	GCWGC	1 cut(s)	1550
Tsp45I	GTSAC	2 cut(s)	1019, 1634
TspGWI	ACGGA	2 cut(s)	1023, 1581
TspRI	CASTG	1 cut(s)	262
Van91I	CCANNNNNTGG	1 cut(s)	293
VpaK11BI	GGWCC	1 cut(s)	1506
XagI	CCTNNNNNAGG	1 cut(s)	864
XapI	RAATTY	2 cut(s)	1064, 1206
XceI	RCATGY	2 cut(s)	620, 1635
XmnI	GAANNNNTTC	1 cut(s)	33
XspI	CTAG	2 cut(s)	696, 735
Zsp2I	ATGCAT	1 cut(s)	622

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.