Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr06

Orthogroup ID

OG0006730

Physical Location & Seq

Genomic Coordinates Forward (+)

722533 .. 725775

Gene Structure 3243 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD06G1007100.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1743 bp

ATGTACTCTGGGAGACCATCTGGTGAAGAAAATCTTCATCAAGATTTTGAAGCTTTGCGTGTATCGAAGTGTCTGATGAGAAATGTTAGCCAGAAGTTGAGGAAGAAGAATCAGAGGTCTGTAGGAGATGAGGACAATGGTGTGAGGGGAATAGCTTTGAGATGTCTTACTCTGTATGGAAGGGGTGGAGGTTGCAAAGTTGGTGCTGACACATGTGATGATTTTGGGGATACAAGTAACAGGAGGAGGTCAAGTGCCAGTGATGAAGGCAAGGGATACAGACCAATATGTGGTACCGAGGAATCTGGAGTGGATTGCTTCTCATATGGGGTGAGGGAGAAATTTTGGAAGAAACATAACAGAAAGGATTTTGAGCTTGAAGAATCAATTATAAATAGCAGGATGCACATTTTTCTTCCAGATGATGTACTGGAAATGTGCTTGGTGAGGCTCCCACTAACCAGTCTCATGACTGCACGCCTTGTGTGCAAGAAATGGAAACACTTGACTACTACTCCTCGGTTCCTGCAGATGAGACGGGAAGGCTCACATCAGACCCCATGGTTGTTTCATTTTGGTGCTGTTAAAGATGATTATTGCTCTGGTGAGATACATGCATTGGATATGTCTCTAAATCAATGGCATAGTATAAATGCTGAAATCCTGAAGGGAAGGTTCATGTTTTCTGTTGCTAGTGTCCAGGATGATATTTATGTTGTTGGGGGTTGTTCAAGCTTGAACAACTTTGGGAGGGTGGATAGGAGCTCTTTTAAGACACACAAAGGGGTGATGCTGTTTAGTCCCTTAACAAAATCTTGGCGCAAAGTTGCTCCAATGAAGTATGCAAGATCAATGCCTATTTTAGGAGTTTCAGAGGTCACTTCAGATTTTTCCACCAGTCAAAGTTATCAAGGTCGCCAAGATAGACGTATTCCCAGATCACGGGTAGGTGGAGTTTCAGAGGTTTATGAAGATCCTCACAGGCTTTCTCTTAGGCGTCAACACAGACATTCTGCTGATGACAATGAGTCTTTGGTGTTGCCCAATAGAAAGTCGTATAAATTTATGAAACAAAAAAGTGATCACTCATCCATGAAGGGTTGTAGAAGGTTTGTGCTCGTTGCTGTTGGTGGTCTTGGCACCTGGGATGAACCTTTGGATTCTGGAGAAATATATGATTCTGTTTCAAGGAAATGGACAGAAATTCAAGGGTTCCCTGTGGACTTTGGGATTGCTTGTTCTGGGGTTGTTTGTAATGGGATGTTTTATATTTATTCTGAAACTGACAAGCTCGCAGGATATGACATAGAAAGGGGTTTCTGGATTGCAATCCAAACCACTCCTTTCCCACCCCGTGTTCATGAATATTACCCAAAACTGGTATCTTGTAATGGCAGGCTCTTCATGCTTTCTGTCTTCTGGTGCGAAGGGGATGGTCAGATAGGCCGGAGAAACAAGGCTGTTAGAAAGGTATGGGAGCTGGATCTCATGTACCTTAACTGGACCGAGGTCTCAGTACATCCCGATGCTCCAATGGACTGGAATGCTGCGTTTGTGGCAGACGGAAACATGATATTCGGTATTGAGATGTTCAAAATATTTGGTCAAGTATTGGATTTTTTTACTGTATGTGATGTGTCTGATACGGGGATGAAATGGAGTCATATCTCAAGGAACCATGTAACTCGCGAGCTGGATGCCTCTTCGTGCTTGACCAAGTCAATGGCTGTGCTACATCTGTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000151 GO:0001558 GO:0005575 GO:0005622 GO:0005623 GO:0008150 GO:0008361 GO:0009987 GO:0016043 GO:0019005 GO:0031461 GO:0031463 GO:0032535 GO:0032991 GO:0040008 GO:0044424 GO:0044464 GO:0050789 GO:0050794 GO:0051128 GO:0065007 GO:0065008 GO:0071840 GO:0090066 GO:1902494 GO:1990234

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

F-box Kelch_1 Kelch_6

Protein Analysis

581

Amino Acids

66.13

Weight (kDa)

8.77

Isoelectric Point (pI)

45.52

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
F-box	PF00646	139 - 176	4e-09	F-box domain
Kelch_KLHDC2_KLHL20_DRC7	PF24681	156 - 242	6.5e-07	KLHDC2/KLHL20/DRC7 Kelch-repeats domain
Kelch_1	PF01344	225 - 280	2.3e-06	Kelch motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0006730)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G42350 AT5G42360
fragaria_vesca	FvH4_5g25640 FvH4_5g25640 FvH4_5g25640 FvH4_5g25640 FvH4_5g25640
malus_domestica	MD00G1113700.v1.1 MD06G1007100.v1.1
prunus_persica	Prupe.5G009000_v2.0.a1 Prupe.5G009000_v2.0.a1 Prupe.5G009000_v2.0.a1 Prupe.5G009000_v2.0.a1
pyrus_communis	pycom06g00620
rosa_chinensis	RchiOBHm_Chr7g0215931
rosa_laevigata	RLG00000002606
rosa_multiflora	Rmu_sc0006240.1_g000003
rosa_roxburghii	Rroxscaffold_3G00243790
rosa_rugosa	Rorug07G0156900 Rorug07G0157000 Rorug07G0157100
rosa_samantha	Rh7AG295000 Rh7BG287000 Rh7CG315100 Rh7DG297800
rosa_wichuraiana	Rw7G025060

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	392
Acc65I	GGTACC	1 cut(s)	293
AccB1I	GGYRCC	2 cut(s)	293, 1139
AccB7I	CCANNNNNTGG	1 cut(s)	290
AccII	CGCG	1 cut(s)	1689
AclWI	GGATC	2 cut(s)	968, 1491
AcsI	RAATTY	3 cut(s)	341, 1061, 1203
AcuI	CTGAAG	2 cut(s)	686, 867
AcyI	GRCGYC	1 cut(s)	997
AdeI	CACNNNGTG	1 cut(s)	1355
AfaI	GTAC	5 cut(s)	5, 295, 429, 1493, 1518
AfiI	CCNNNNNNNGG	4 cut(s)	290, 863, 942, 1378
AflIII	ACRYGT	1 cut(s)	212
AgsI	TTSAA	7 cut(s)	50, 380, 732, 739, 1188, 1208, 1594
AhdI	GACNNNNNGTC	1 cut(s)	1027
AjnI	CCWGG	2 cut(s)	699, 1142
AloI	GAACNNNNNNTCC	2 cut(s)	1222, 1254
AluBI	AGCT	8 cut(s)	53, 155, 376, 735, 765, 1291, 1480, 1693
AluI	AGCT	8 cut(s)	53, 155, 376, 735, 765, 1291, 1480, 1693
Alw21I	GWGCWC	2 cut(s)	767, 1119
Alw26I	GTCTC	5 cut(s)	7, 470, 529, 633, 1516
AlwI	GGATC	2 cut(s)	968, 1491
AoxI	GGCC	1 cut(s)	1444
ApeKI	GCWGC	1 cut(s)	1547
ApoI	RAATTY	3 cut(s)	341, 1061, 1203
Asp700I	GAANNNNTTC	1 cut(s)	33
Asp718I	GGTACC	1 cut(s)	293
AspLEI	GCGC	1 cut(s)	822
AspS9I	GGNCC	1 cut(s)	1503
AsuHPI	GGTGA	5 cut(s)	35, 343, 457, 617, 799
AvaII	GGWCC	1 cut(s)	1503
BanI	GGYRCC	2 cut(s)	293, 1139
BanII	GRGCYC	1 cut(s)	767
BbsI	GAAGAC	1 cut(s)	1408
Bbv12I	GWGCWC	2 cut(s)	767, 1119
BbvI	GCAGC	1 cut(s)	1534
BccI	CCATC	2 cut(s)	25, 1427
BcgI	CGANNNNNNTGC	2 cut(s)	1679, 1713
BciT130I	CCWGG	2 cut(s)	701, 1144
BciVI	GTATCC	2 cut(s)	223, 269
BclI	TGATCA	1 cut(s)	1081
BcoDI	GTCTC	5 cut(s)	7, 470, 529, 633, 1516
BfaI	CTAG	1 cut(s)	693
BfmI	CTRYAG	2 cut(s)	120, 527
BfuI	GTATCC	2 cut(s)	223, 269
BisI	GCNGC	1 cut(s)	1548
BlsI	GCNGC	1 cut(s)	1549
Bme1390I	CCNGG	2 cut(s)	701, 1144
Bme18I	GGWCC	1 cut(s)	1503
BmeRI	GACNNNNNGTC	1 cut(s)	1027
BmgT120I	GGNCC	1 cut(s)	1503
BmiI	GGNNCC	6 cut(s)	295, 452, 524, 1141, 1214, 1676
BmrFI	CCNGG	2 cut(s)	701, 1144
BmsI	GCATC	4 cut(s)	393, 780, 1516, 1687
BoxI	GACNNNNGTC	1 cut(s)	1508
BpiI	GAAGAC	1 cut(s)	1408
BpmI	CTGGAG	2 cut(s)	327, 1185
BpuEI	CTTGAG	1 cut(s)	1654
BsaBI	GATNNNNATC	1 cut(s)	1328
BsaHI	GRCGYC	1 cut(s)	997
BsaI	GGTCTC	2 cut(s)	7, 1516
BsaJI	CCNNGG	5 cut(s)	297, 518, 560, 1143, 1506
BsaXI	ACNNNNNCTCC	4 cut(s)	238, 268, 499, 529
Bsc4I	CCNNNNNNNGG	4 cut(s)	290, 863, 942, 1378
Bse1I	ACTGG	7 cut(s)	258, 435, 462, 897, 1383, 1505, 1544
Bse8I	GATNNNNATC	1 cut(s)	1328
BseBI	CCWGG	2 cut(s)	701, 1144
BseDI	CCNNGG	5 cut(s)	297, 518, 560, 1143, 1506
BseGI	GGATG	9 cut(s)	408, 709, 1088, 1153, 1266, 1438, 1519, 1656, 1702
BseJI	GATNNNNATC	1 cut(s)	1328
BseLI	CCNNNNNNNGG	4 cut(s)	290, 863, 942, 1378
BseMII	CTCAG	1 cut(s)	1527
BseNI	ACTGG	7 cut(s)	258, 435, 462, 897, 1383, 1505, 1544
BseRI	GAGGAG	2 cut(s)	259, 507
BseXI	GCAGC	1 cut(s)	1534
BsgI	GTGCAG	1 cut(s)	459
Bsh1236I	CGCG	1 cut(s)	1689
BshFI	GGCC	1 cut(s)	1446
BshNI	GGYRCC	2 cut(s)	293, 1139
BsiHKAI	GWGCWC	2 cut(s)	767, 1119
BsiSI	CCGG	1 cut(s)	1447
BslFI	GGGAC	1 cut(s)	786
BslI	CCNNNNNNNGG	4 cut(s)	290, 863, 942, 1378
BsmAI	GTCTC	5 cut(s)	7, 470, 529, 633, 1516
BsmBI	CGTCTC	1 cut(s)	529
BsmFI	GGGAC	1 cut(s)	786
BsmI	GAATGC	1 cut(s)	1549
BsnI	GGCC	1 cut(s)	1446
Bso31I	GGTCTC	2 cut(s)	7, 1516
Bsp1286I	GDGCHC	2 cut(s)	767, 1119
Bsp143I	GATC	5 cut(s)	848, 938, 973, 1081, 1483
Bsp19I	CCATGG	1 cut(s)	560
Bsp68I	TCGCGA	1 cut(s)	1689
BspANI	GGCC	1 cut(s)	1446
BspCNI	CTCAG	1 cut(s)	1526
BspFNI	CGCG	1 cut(s)	1689
BspHI	TCATGA	2 cut(s)	468, 1360
BspLI	GGNNCC	6 cut(s)	295, 452, 524, 1141, 1214, 1676
BspMAI	CTGCAG	1 cut(s)	531
BspPI	GGATC	2 cut(s)	968, 1491
BspQI	GCTCTTC	1 cut(s)	1406
BspT107I	GGYRCC	2 cut(s)	293, 1139
BspTNI	GGTCTC	2 cut(s)	7, 1516
BsrI	ACTGG	7 cut(s)	258, 435, 462, 897, 1383, 1505, 1544
BssECI	CCNNGG	5 cut(s)	297, 518, 560, 1143, 1506
BssMI	GATC	5 cut(s)	848, 938, 973, 1081, 1483
BssNI	GRCGYC	1 cut(s)	997
BssT1I	CCWWGG	1 cut(s)	560
Bst2UI	CCWGG	2 cut(s)	701, 1144
Bst4CI	ACNGT	1 cut(s)	1627
Bst6I	CTCTTC	2 cut(s)	1406, 1708
BstACI	GRCGYC	1 cut(s)	997
BstC8I	GCNNGC	4 cut(s)	478, 1293, 1397, 1691
BstDEI	CTNAG	2 cut(s)	992, 1513
BstDSI	CCRYGG	1 cut(s)	560
BstENI	CCTNNNNNAGG	1 cut(s)	861
BstF5I	GGATG	9 cut(s)	408, 709, 1088, 1153, 1266, 1438, 1519, 1656, 1702
BstFNI	CGCG	1 cut(s)	1689
BstHHI	GCGC	1 cut(s)	822
BstKTI	GATC	5 cut(s)	851, 941, 976, 1084, 1486
BstMAI	GTCTC	5 cut(s)	7, 470, 529, 633, 1516
BstMBI	GATC	5 cut(s)	848, 938, 973, 1081, 1483
BstMWI	GCNNNNNNNGC	3 cut(s)	486, 1405, 1556
BstNI	CCWGG	2 cut(s)	701, 1144
BstNSI	RCATGY	2 cut(s)	216, 617
BstPAI	GACNNNNGTC	1 cut(s)	1508
BstSCI	CCNGG	2 cut(s)	699, 1142
BstSFI	CTRYAG	2 cut(s)	120, 527
BstUI	CGCG	1 cut(s)	1689
BstV1I	GCAGC	1 cut(s)	1534
BstV2I	GAAGAC	1 cut(s)	1408
BstX2I	RGATCY	2 cut(s)	973, 1483
BstXI	CCANNNNNNTGG	2 cut(s)	1539, 1723
BstYI	RGATCY	2 cut(s)	973, 1483
BsuI	GTATCC	2 cut(s)	223, 269
BsuRI	GGCC	1 cut(s)	1446
BtgI	CCRYGG	1 cut(s)	560
BtsCI	GGATG	9 cut(s)	408, 709, 1088, 1153, 1266, 1438, 1519, 1656, 1702
BtsIMutI	CAGTG	1 cut(s)	265
BtuMI	TCGCGA	1 cut(s)	1689
Cac8I	GCNNGC	4 cut(s)	478, 1293, 1397, 1691
CciI	TCATGA	2 cut(s)	468, 1360
CfoI	GCGC	1 cut(s)	822
Cfr13I	GGNCC	1 cut(s)	1503
CseI	GACGC	1 cut(s)	986
Csp6I	GTAC	5 cut(s)	4, 294, 428, 1492, 1517
CviQI	GTAC	5 cut(s)	4, 294, 428, 1492, 1517
DdeI	CTNAG	2 cut(s)	992, 1513
DpnI	GATC	5 cut(s)	850, 940, 975, 1083, 1485
DpnII	GATC	5 cut(s)	848, 938, 973, 1081, 1483
DraIII	CACNNNGTG	1 cut(s)	1355
DriI	GACNNNNNGTC	1 cut(s)	1027
Eam1104I	CTCTTC	2 cut(s)	1406, 1708
Eam1105I	GACNNNNNGTC	1 cut(s)	1027
EarI	CTCTTC	2 cut(s)	1406, 1708
Ecl136II	GAGCTC	1 cut(s)	765
Eco130I	CCWWGG	1 cut(s)	560
Eco24I	GRGCYC	1 cut(s)	767
Eco31I	GGTCTC	2 cut(s)	7, 1516
Eco47I	GGWCC	1 cut(s)	1503
Eco53kI	GAGCTC	1 cut(s)	765
Eco57I	CTGAAG	2 cut(s)	686, 867
EcoICRI	GAGCTC	1 cut(s)	765
EcoNI	CCTNNNNNAGG	1 cut(s)	861
EcoRII	CCWGG	2 cut(s)	699, 1142
EcoT14I	CCWWGG	1 cut(s)	560
EcoT22I	ATGCAT	1 cut(s)	619
EcoT38I	GRGCYC	1 cut(s)	767
ErhI	CCWWGG	1 cut(s)	560
Esp3I	CGTCTC	1 cut(s)	529
FalI	AAGNNNNNCTT	2 cut(s)	18, 50
FaqI	GGGAC	1 cut(s)	786
FauNDI	CATATG	1 cut(s)	325
FbaI	TGATCA	1 cut(s)	1081
Fnu4HI	GCNGC	1 cut(s)	1548
FokI	GGATG	9 cut(s)	415, 716, 1075, 1160, 1273, 1445, 1506, 1663, 1709
FriOI	GRGCYC	1 cut(s)	767
Fsp4HI	GCNGC	1 cut(s)	1548
FspBI	CTAG	1 cut(s)	693
GlaI	GCGC	1 cut(s)	821
GluI	GCNGC	1 cut(s)	1548
GsuI	CTGGAG	2 cut(s)	327, 1185
HaeIII	GGCC	1 cut(s)	1446
HapII	CCGG	1 cut(s)	1447
HgaI	GACGC	1 cut(s)	986
HhaI	GCGC	1 cut(s)	822
Hin1I	GRCGYC	1 cut(s)	997
Hin6I	GCGC	1 cut(s)	820
HinP1I	GCGC	1 cut(s)	820
HincII	GTYRAC	1 cut(s)	1001
HindII	GTYRAC	1 cut(s)	1001
HindIII	AAGCTT	2 cut(s)	51, 733
HinfI	GANTC	7 cut(s)	109, 302, 383, 1028, 1160, 1178, 1660
HpaII	CCGG	1 cut(s)	1447
HphI	GGTGA	5 cut(s)	35, 343, 457, 617, 799
Hpy166II	GTNNAC	2 cut(s)	1001, 1222
Hpy188I	TCNGA	9 cut(s)	75, 114, 555, 874, 886, 961, 1279, 1440, 1642
Hpy8I	GTNNAC	2 cut(s)	1001, 1222
HpyAV	CCTTC	8 cut(s)	174, 260, 536, 661, 666, 1090, 1101, 1421
HpyCH4III	ACNGT	1 cut(s)	1627
HpyCH4IV	ACGT	1 cut(s)	928
HpyCH4V	TGCA	8 cut(s)	195, 406, 476, 489, 529, 617, 845, 1328
HpyF10VI	GCNNNNNNNGC	3 cut(s)	486, 1405, 1556
HpyF3I	CTNAG	2 cut(s)	992, 1513
HpySE526I	ACGT	1 cut(s)	928
Hsp92I	GRCGYC	1 cut(s)	997
HspAI	GCGC	1 cut(s)	820
KpnI	GGTACC	1 cut(s)	297
Ksp22I	TGATCA	1 cut(s)	1081
Kzo9I	GATC	5 cut(s)	848, 938, 973, 1081, 1483
LguI	GCTCTTC	1 cut(s)	1406
LmnI	GCTCC	5 cut(s)	456, 762, 835, 1477, 1534
Lsp1109I	GCAGC	1 cut(s)	1534
LweI	GCATC	4 cut(s)	393, 780, 1516, 1687
MaeI	CTAG	1 cut(s)	693
MaeII	ACGT	1 cut(s)	928
MaeIII	GTNAC	3 cut(s)	236, 877, 1681
MalI	GATC	5 cut(s)	850, 940, 975, 1083, 1485
MboI	GATC	5 cut(s)	848, 938, 973, 1081, 1483
MflI	RGATCY	2 cut(s)	973, 1483
MhlI	GDGCHC	2 cut(s)	767, 1119
MluCI	AATT	4 cut(s)	341, 387, 1061, 1203
MlyI	GAGTC	2 cut(s)	1037, 1669
Mph1103I	ATGCAT	1 cut(s)	619
MroXI	GAANNNNTTC	1 cut(s)	33
MseI	TTAA	4 cut(s)	585, 771, 806, 1497
MslI	CAYNNNNRTG	2 cut(s)	576, 1524
MspI	CCGG	1 cut(s)	1447
MspR9I	CCNGG	2 cut(s)	701, 1144
Mva1269I	GAATGC	1 cut(s)	1549
MvaI	CCWGG	2 cut(s)	701, 1144
MvnI	CGCG	1 cut(s)	1689
MwoI	GCNNNNNNNGC	3 cut(s)	486, 1405, 1556
NcoI	CCATGG	1 cut(s)	560
NdeI	CATATG	1 cut(s)	325
NdeII	GATC	5 cut(s)	848, 938, 973, 1081, 1483
NlaIV	GGNNCC	6 cut(s)	295, 452, 524, 1141, 1214, 1676
NmuCI	GTSAC	1 cut(s)	877
NruI	TCGCGA	1 cut(s)	1689
NsiI	ATGCAT	1 cut(s)	619
NspI	RCATGY	2 cut(s)	216, 617
PagI	TCATGA	2 cut(s)	468, 1360
PciI	ACATGT	1 cut(s)	212
PciSI	GCTCTTC	1 cut(s)	1406
PctI	GAATGC	1 cut(s)	1549
PdmI	GAANNNNTTC	1 cut(s)	33
PfeI	GAWTC	5 cut(s)	109, 302, 383, 1160, 1178
PflFI	GACNNNGTC	1 cut(s)	1717
PflMI	CCANNNNNTGG	1 cut(s)	290
PfoI	TCCNGGA	1 cut(s)	699
PkrI	GCNGC	1 cut(s)	1549
PleI	GAGTC	2 cut(s)	1036, 1668
PpsI	GAGTC	2 cut(s)	1036, 1668
PscI	ACATGT	1 cut(s)	212
PshAI	GACNNNNGTC	1 cut(s)	1508
PsiI	TTATAA	1 cut(s)	392
Psp124BI	GAGCTC	1 cut(s)	767
Psp6I	CCWGG	2 cut(s)	699, 1142
PspGI	CCWGG	2 cut(s)	699, 1142
PspN4I	GGNNCC	6 cut(s)	295, 452, 524, 1141, 1214, 1676
PspPI	GGNCC	1 cut(s)	1503
PstI	CTGCAG	1 cut(s)	531
PsuI	RGATCY	2 cut(s)	973, 1483
PsyI	GACNNNGTC	1 cut(s)	1717
RruI	TCGCGA	1 cut(s)	1689
RsaI	GTAC	5 cut(s)	5, 295, 429, 1493, 1518
RsaNI	GTAC	5 cut(s)	4, 294, 428, 1492, 1517
RseI	CAYNNNNRTG	2 cut(s)	576, 1524
SacI	GAGCTC	1 cut(s)	767
SapI	GCTCTTC	1 cut(s)	1406
SaqAI	TTAA	4 cut(s)	585, 771, 806, 1497
SatI	GCNGC	1 cut(s)	1548
Sau3AI	GATC	5 cut(s)	848, 938, 973, 1081, 1483
Sau96I	GGNCC	1 cut(s)	1503
SchI	GAGTC	2 cut(s)	1037, 1669
ScrFI	CCNGG	2 cut(s)	701, 1144
SduI	GDGCHC	2 cut(s)	767, 1119
SfaNI	GCATC	4 cut(s)	393, 780, 1516, 1687
SfcI	CTRYAG	2 cut(s)	120, 527
SinI	GGWCC	1 cut(s)	1503
SmiMI	CAYNNNNRTG	2 cut(s)	576, 1524
SmlI	CTYRAG	1 cut(s)	1669
SmoI	CTYRAG	1 cut(s)	1669
Sse9I	AATT	4 cut(s)	341, 387, 1061, 1203
SspI	AATATT	2 cut(s)	1367, 1599
SspMI	CTAG	1 cut(s)	693
SstI	GAGCTC	1 cut(s)	767
StyD4I	CCNGG	2 cut(s)	699, 1142
StyI	CCWWGG	1 cut(s)	560
TaaI	ACNGT	1 cut(s)	1627
TaiI	ACGT	1 cut(s)	931
TaqI	TCGA	1 cut(s)	65
TaqII	GACCGA	1 cut(s)	1520
TasI	AATT	4 cut(s)	341, 387, 1061, 1203
TatI	WGTACW	3 cut(s)	3, 427, 1516
TfiI	GAWTC	5 cut(s)	109, 302, 383, 1160, 1178
Tru1I	TTAA	4 cut(s)	585, 771, 806, 1497
Tru9I	TTAA	4 cut(s)	585, 771, 806, 1497
TscAI	CASTG	1 cut(s)	265
TseFI	GTSAC	1 cut(s)	877
TseI	GCWGC	1 cut(s)	1547
Tsp45I	GTSAC	1 cut(s)	877
TspGWI	ACGGA	1 cut(s)	1578
TspRI	CASTG	1 cut(s)	265
Tth111I	GACNNNGTC	1 cut(s)	1717
Van91I	CCANNNNNTGG	1 cut(s)	290
VpaK11BI	GGWCC	1 cut(s)	1503
XagI	CCTNNNNNAGG	1 cut(s)	861
XapI	RAATTY	3 cut(s)	341, 1061, 1203
XceI	RCATGY	2 cut(s)	216, 617
XmnI	GAANNNNTTC	1 cut(s)	33
XspI	CTAG	1 cut(s)	693
Zsp2I	ATGCAT	1 cut(s)	619

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.