Rorug07G0157100

F-box kelch-repeat protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006730
Physical Location & Seq
Reverse (-)
12476076 .. 12479446
3371 bp
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UTR
Exon/CDS
Intron
Rorug07G0157100.1

Sequence Viewer

Length: 549 bp
ATGCCCGCGCGTTGCTGCGGGATTTGGATCGAGTTTGCAGATAATTTTCAGTTCCCGGCGGAGGCGGAGAGGCTGGAGGAGGTGGCGGCGCAGGTGGCGGAGCTGGCGGATATTTGCAATAGGATGGAGGAAGGGTTGGTGCCTCTGCAGCAGCAGATAAGGGAACTGTTCCACAGGGCCGTGAGGAGCAGGACGGAGGTGCTTGAGGTTTTGGACCAAGGTGGTAAAGGAGTAATCGGTGTTTCGGATGATGTTCTCTGGGCAGATGCGTCTTTGGACAGCGGTTTTCAGTTTTGTAGTGGTGTCCGACACGATGTCGGCTGGGTCTATCCGTCTTTAGAAAGAGAAGTAAGAGTGTTTGGTTTCGGGAGAGTGAGGATTGCAGAAGCACAATCTGATCCAGTGCTTGGCAGTCAATCTATAAGTGACAGAGGGGCTCAAATGCCAGTGGGTCGAGATGTTCAGTCGTTTCGAATTTTTGTAGCAACCTGGAATGTAGGAGGGAAATCTCCCCATAGTGGCTTCTATGCTTTTGAATTTGGTCCTTAA

Protein Analysis

182

Amino Acids

20.18

Weight (kDa)

4.81

Isoelectric Point (pI)

46.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ROH1-like PF05633 10 - 71 7.3e-20 Protein ROH1-like
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

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Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 82
Acc36I ACCTGC 1 cut(s) 82
AccB1I GGYRCC 1 cut(s) 139
AccB7I CCANNNNNTGG 1 cut(s) 407
AccII CGCG 2 cut(s) 8, 10
AciI CCGC 8 cut(s) 6, 18, 59, 65, 86, 98, 107, 282
AclWI GGATC 2 cut(s) 35, 392
AcsI RAATTY 2 cut(s) 474, 536
AfiI CCNNNNNNNGG 3 cut(s) 61, 407, 518
AgsI TTSAA 1 cut(s) 536
AhdI GACNNNNNGTC 1 cut(s) 314
AjnI CCWGG 1 cut(s) 488
AluBI AGCT 1 cut(s) 103
AluI AGCT 1 cut(s) 103
AlwI GGATC 2 cut(s) 35, 392
AoxI GGCC 1 cut(s) 177
ApeKI GCWGC 3 cut(s) 15, 148, 151
ApoI RAATTY 2 cut(s) 474, 536
AspLEI GCGC 2 cut(s) 10, 91
AspS9I GGNCC 3 cut(s) 177, 214, 542
AsuC2I CCSGG 1 cut(s) 56
AsuII TTCGAA 1 cut(s) 472
AvaII GGWCC 2 cut(s) 214, 542
BanI GGYRCC 1 cut(s) 139
BanII GRGCYC 1 cut(s) 439
BbvI GCAGC 3 cut(s) 2, 160, 163
BccI CCATC 1 cut(s) 118
BceAI ACGGC 1 cut(s) 164
BciT130I CCWGG 1 cut(s) 490
BcnI CCSGG 1 cut(s) 56
BfmI CTRYAG 1 cut(s) 146
BfuAI ACCTGC 1 cut(s) 82
BisI GCNGC 4 cut(s) 16, 87, 149, 152
BlsI GCNGC 4 cut(s) 17, 88, 150, 153
Bme1390I CCNGG 2 cut(s) 56, 490
Bme18I GGWCC 2 cut(s) 214, 542
BmeRI GACNNNNNGTC 1 cut(s) 314
BmgT120I GGNCC 3 cut(s) 177, 214, 542
BmiI GGNNCC 1 cut(s) 141
BmrFI CCNGG 2 cut(s) 56, 490
BmsI GCATC 1 cut(s) 256
BpmI CTGGAG 1 cut(s) 95
Bpu14I TTCGAA 1 cut(s) 472
BpuEI CTTGAG 1 cut(s) 224
BpuMI CCSGG 1 cut(s) 56
BsaBI GATNNNNATC 1 cut(s) 26
BsaJI CCNNGG 1 cut(s) 217
BsaXI ACNNNNNCTCC 2 cut(s) 119, 149
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 407, 518
Bse1I ACTGG 2 cut(s) 401, 446
Bse8I GATNNNNATC 1 cut(s) 26
BseBI CCWGG 1 cut(s) 490
BseDI CCNNGG 1 cut(s) 217
BseGI GGATG 2 cut(s) 129, 253
BseJI GATNNNNATC 1 cut(s) 26
BseLI CCNNNNNNNGG 3 cut(s) 61, 407, 518
BseNI ACTGG 2 cut(s) 401, 446
BseRI GAGGAG 2 cut(s) 92, 199
BseXI GCAGC 3 cut(s) 2, 160, 163
BseYI CCCAGC 1 cut(s) 321
Bsh1236I CGCG 2 cut(s) 8, 10
BshFI GGCC 1 cut(s) 179
BshNI GGYRCC 1 cut(s) 139
BsiSI CCGG 1 cut(s) 56
BslI CCNNNNNNNGG 3 cut(s) 61, 407, 518
BsnI GGCC 1 cut(s) 179
Bsp119I TTCGAA 1 cut(s) 472
Bsp1286I GDGCHC 1 cut(s) 439
Bsp143I GATC 2 cut(s) 27, 397
BspACI CCGC 8 cut(s) 6, 18, 59, 65, 86, 98, 107, 282
BspANI GGCC 1 cut(s) 179
BspFNI CGCG 2 cut(s) 8, 10
BspLI GGNNCC 1 cut(s) 141
BspMAI CTGCAG 1 cut(s) 150
BspMI ACCTGC 1 cut(s) 82
BspPI GGATC 2 cut(s) 35, 392
BspT104I TTCGAA 1 cut(s) 472
BspT107I GGYRCC 1 cut(s) 139
BsrI ACTGG 2 cut(s) 401, 446
BssECI CCNNGG 1 cut(s) 217
BssMI GATC 2 cut(s) 27, 397
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 490
Bst4CI ACNGT 1 cut(s) 168
BstBI TTCGAA 1 cut(s) 472
BstC8I GCNNGC 2 cut(s) 6, 105
BstF5I GGATG 2 cut(s) 129, 253
BstFNI CGCG 2 cut(s) 8, 10
BstHHI GCGC 2 cut(s) 10, 91
BstKTI GATC 2 cut(s) 30, 400
BstMBI GATC 2 cut(s) 27, 397
BstMWI GCNNNNNNNGC 3 cut(s) 95, 104, 148
BstNI CCWGG 1 cut(s) 490
BstSCI CCNGG 2 cut(s) 54, 488
BstSFI CTRYAG 1 cut(s) 146
BstUI CGCG 2 cut(s) 8, 10
BstV1I GCAGC 3 cut(s) 2, 160, 163
BsuRI GGCC 1 cut(s) 179
BtsCI GGATG 2 cut(s) 129, 253
BtsIMutI CAGTG 2 cut(s) 408, 453
BveI ACCTGC 1 cut(s) 82
Cac8I GCNNGC 2 cut(s) 6, 105
CfoI GCGC 2 cut(s) 10, 91
Cfr13I GGNCC 3 cut(s) 177, 214, 542
CseI GACGC 1 cut(s) 258
CviJI RGCY 6 cut(s) 73, 103, 179, 321, 437, 522
CviKI_1 RGCY 6 cut(s) 73, 103, 179, 321, 437, 522
DpnI GATC 2 cut(s) 29, 399
DpnII GATC 2 cut(s) 27, 397
DriI GACNNNNNGTC 1 cut(s) 314
Eam1105I GACNNNNNGTC 1 cut(s) 314
EciI GGCGGA 4 cut(s) 74, 80, 113, 122
Eco130I CCWWGG 1 cut(s) 217
Eco24I GRGCYC 1 cut(s) 439
Eco47I GGWCC 2 cut(s) 214, 542
EcoRII CCWGG 1 cut(s) 488
EcoT14I CCWWGG 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 439
ErhI CCWWGG 1 cut(s) 217
FaiI YATR 3 cut(s) 422, 516, 528
FauI CCCGC 2 cut(s) 11, 13
Fnu4HI GCNGC 4 cut(s) 16, 87, 149, 152
FokI GGATG 2 cut(s) 136, 260
FriOI GRGCYC 1 cut(s) 439
Fsp4HI GCNGC 4 cut(s) 16, 87, 149, 152
GlaI GCGC 2 cut(s) 9, 90
GluI GCNGC 4 cut(s) 16, 87, 149, 152
GsaI CCCAGC 1 cut(s) 325
GsuI CTGGAG 1 cut(s) 95
HaeIII GGCC 1 cut(s) 179
HapII CCGG 1 cut(s) 56
HgaI GACGC 1 cut(s) 258
HhaI GCGC 2 cut(s) 10, 91
Hin6I GCGC 2 cut(s) 8, 89
HinP1I GCGC 2 cut(s) 8, 89
HpaII CCGG 1 cut(s) 56
Hpy188I TCNGA 3 cut(s) 247, 308, 397
Hpy188III TCNNGA 2 cut(s) 367, 455
HpyAV CCTTC 1 cut(s) 125
HpyCH4III ACNGT 1 cut(s) 168
HpyCH4V TGCA 4 cut(s) 38, 117, 148, 383
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 104, 148
HspAI GCGC 2 cut(s) 8, 89
Kzo9I GATC 2 cut(s) 27, 397
LmnI GCTCC 2 cut(s) 100, 186
Lsp1109I GCAGC 3 cut(s) 2, 160, 163
LweI GCATC 1 cut(s) 256
MaeIII GTNAC 1 cut(s) 425
MalI GATC 2 cut(s) 29, 399
MboI GATC 2 cut(s) 27, 397
MhlI GDGCHC 1 cut(s) 439
MluCI AATT 3 cut(s) 43, 474, 536
MmeI TCCRAC 1 cut(s) 331
MseI TTAA 1 cut(s) 547
MspA1I CMGCKG 1 cut(s) 282
MspI CCGG 1 cut(s) 56
MspR9I CCNGG 2 cut(s) 56, 490
MvaI CCWGG 1 cut(s) 490
MvnI CGCG 2 cut(s) 8, 10
MwoI GCNNNNNNNGC 3 cut(s) 95, 104, 148
NciI CCSGG 1 cut(s) 56
NdeII GATC 2 cut(s) 27, 397
NlaIV GGNNCC 1 cut(s) 141
NmuCI GTSAC 1 cut(s) 425
NspV TTCGAA 1 cut(s) 472
PaqCI CACCTGC 1 cut(s) 82
PflMI CCANNNNNTGG 1 cut(s) 407
PkrI GCNGC 4 cut(s) 17, 88, 150, 153
Psp6I CCWGG 1 cut(s) 488
PspFI CCCAGC 1 cut(s) 321
PspGI CCWGG 1 cut(s) 488
PspN4I GGNNCC 1 cut(s) 141
PspPI GGNCC 3 cut(s) 177, 214, 542
PstI CTGCAG 1 cut(s) 150
SaqAI TTAA 1 cut(s) 547
SatI GCNGC 4 cut(s) 16, 87, 149, 152
Sau3AI GATC 2 cut(s) 27, 397
Sau96I GGNCC 3 cut(s) 177, 214, 542
ScrFI CCNGG 2 cut(s) 56, 490
SduI GDGCHC 1 cut(s) 439
SetI ASST 7 cut(s) 84, 96, 105, 201, 210, 223, 491
SfaNI GCATC 1 cut(s) 256
SfcI CTRYAG 1 cut(s) 146
SfuI TTCGAA 1 cut(s) 472
SinI GGWCC 2 cut(s) 214, 542
SmlI CTYRAG 1 cut(s) 203
SmoI CTYRAG 1 cut(s) 203
Sse9I AATT 3 cut(s) 43, 474, 536
SsiI CCGC 8 cut(s) 6, 18, 59, 65, 86, 98, 107, 282
StyD4I CCNGG 2 cut(s) 54, 488
StyI CCWWGG 1 cut(s) 217
TaaI ACNGT 1 cut(s) 168
TaqI TCGA 3 cut(s) 30, 454, 472
TasI AATT 3 cut(s) 43, 474, 536
TauI GCSGC 1 cut(s) 89
Tru1I TTAA 1 cut(s) 547
Tru9I TTAA 1 cut(s) 547
TscAI CASTG 2 cut(s) 408, 453
TseFI GTSAC 1 cut(s) 425
TseI GCWGC 3 cut(s) 15, 148, 151
Tsp45I GTSAC 1 cut(s) 425
TspGWI ACGGA 2 cut(s) 209, 321
TspRI CASTG 2 cut(s) 408, 453
Van91I CCANNNNNTGG 1 cut(s) 407
VpaK11BI GGWCC 2 cut(s) 214, 542
XapI RAATTY 2 cut(s) 474, 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.