Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0013993

Physical Location & Seq

Genomic Coordinates Reverse (-)

32846897 .. 32847429

Gene Structure 533 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1149400.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 447 bp

ATGATATACGAGTTCATGCCGAACGGGAACCTTGGGACCGCGTTGCATGGAAATCAAGCCGGAAAGCTGTTGGTCGACTGGGTTTCGAGGTATAACATAGCAGTGGGTGTTGCGCAAGCTCCGAATTATCTCCACCATGACTGTCAGCCGCCAGTTATTCACCGAGATATCAAATCGAACAACATTCTGCTGGATACAAACCTTGACGCCAGAGTGGCGGATTTCGGGTTAGCAAGGACAATGATCCACAAGAACGAGACAGTTTCGATGGTAGCGGGATCTTACGGATACATTGCACCTGAATATGGATACGCGTTGAAGGTAGACGAAAAGACTGACATCTACAGCTACGGCGTAGTTCTGTTAGAGCTTATAACCGGAAAGATGCATGTAGACCCTTCGTTCGGGGAATCTGTGGATATCGTGGAGTGGTTCGAAGGAAGATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0007275 GO:0008150 GO:0009790 GO:0009791 GO:0009793 GO:0009888 GO:0010065 GO:0010067 GO:0010087 GO:0010154 GO:0022414 GO:0032501 GO:0032502 GO:0048316 GO:0048507 GO:0048508 GO:0048608 GO:0048731 GO:0048856 GO:0061458

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

LRRNT_2 LRR_1 LRR_6 LRR_8 Pkinase

Protein Analysis

149

Amino Acids

16.49

Weight (kDa)

5.2

Isoelectric Point (pI)

19.89

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Pkinase	PF00069	1 - 131	3.8e-26	Protein kinase domain
PK_Tyr_Ser-Thr	PF07714	1 - 130	5.8e-24	Protein tyrosine and serine/threonine kinase

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready

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Orthologous Genes (Group: OG0013993)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G08590
fragaria_vesca	FvH4_6g01540 FvH4_6g20240
malus_domestica	MD00G1149400.v1.1 MD04G1232500.v1.1
prunus_persica	Prupe.6G352100_v2.0.a1
rosa_chinensis	RchiOBHm_Chr3g0449301
rosa_laevigata	RLG00000025841
rosa_multiflora	Rmu_sc0007719.1_g000004 Rmu_sc0018234.1_g000001
rosa_roxburghii	Rroxscaffold_6G00427300
rosa_rugosa	Rorug02G0619500
rosa_samantha	Rh3AG017500 Rh3BG017600 Rh3CG016600 Rh3DG017900
rosa_wichuraiana	Rw3G001340

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	374
Acc16I	TGCGCA	1 cut(s)	114
AccI	GTMKAC	3 cut(s)	75, 324, 393
AccII	CGCG	2 cut(s)	41, 314
AciI	CCGC	4 cut(s)	39, 149, 218, 275
AclWI	GGATC	2 cut(s)	238, 286
AcyI	GRCGYC	1 cut(s)	207
AfiI	CCNNNNNNNGG	2 cut(s)	305, 404
AflIII	ACRYGT	1 cut(s)	312
AgsI	TTSAA	1 cut(s)	319
AluBI	AGCT	4 cut(s)	67, 119, 348, 370
AluI	AGCT	4 cut(s)	67, 119, 348, 370
Alw26I	GTCTC	1 cut(s)	251
AlwI	GGATC	2 cut(s)	238, 286
ArsI	GACNNNNNNTTYG	2 cut(s)	386, 418
AspLEI	GCGC	1 cut(s)	115
AspS9I	GGNCC	1 cut(s)	36
AsuHPI	GGTGA	1 cut(s)	152
AsuII	TTCGAA	1 cut(s)	435
AvaII	GGWCC	1 cut(s)	36
BccI	CCATC	1 cut(s)	262
BceAI	ACGGC	1 cut(s)	367
BciVI	GTATCC	3 cut(s)	187, 281, 302
BcoDI	GTCTC	1 cut(s)	251
BfmI	CTRYAG	1 cut(s)	343
BfuI	GTATCC	3 cut(s)	187, 281, 302
BglI	GCCNNNNNGGC	1 cut(s)	215
BisI	GCNGC	1 cut(s)	149
BlsI	GCNGC	1 cut(s)	150
Bme18I	GGWCC	1 cut(s)	36
BmgT120I	GGNCC	1 cut(s)	36
BmiI	GGNNCC	2 cut(s)	29, 37
BmrI	ACTGGG	1 cut(s)	88
BmsI	GCATC	1 cut(s)	375
BmuI	ACTGGG	1 cut(s)	88
Bpu14I	TTCGAA	1 cut(s)	435
BsaHI	GRCGYC	1 cut(s)	207
BsaJI	CCNNGG	1 cut(s)	31
BsaWI	WCCGGW	1 cut(s)	377
Bsc4I	CCNNNNNNNGG	2 cut(s)	305, 404
Bse1I	ACTGG	2 cut(s)	83, 152
Bse3DI	GCAATG	1 cut(s)	291
BseDI	CCNNGG	1 cut(s)	31
BseLI	CCNNNNNNNGG	2 cut(s)	305, 404
BseMI	GCAATG	1 cut(s)	291
BseNI	ACTGG	2 cut(s)	83, 152
Bsh1236I	CGCG	2 cut(s)	41, 314
BsiSI	CCGG	2 cut(s)	60, 378
BslFI	GGGAC	1 cut(s)	49
BslI	CCNNNNNNNGG	2 cut(s)	305, 404
BsmAI	GTCTC	1 cut(s)	251
BsmFI	GGGAC	1 cut(s)	49
Bsp119I	TTCGAA	1 cut(s)	435
Bsp143I	GATC	2 cut(s)	243, 278
BspACI	CCGC	4 cut(s)	39, 149, 218, 275
BspFNI	CGCG	2 cut(s)	41, 314
BspLI	GGNNCC	2 cut(s)	29, 37
BspPI	GGATC	2 cut(s)	238, 286
BspT104I	TTCGAA	1 cut(s)	435
BsrDI	GCAATG	1 cut(s)	291
BsrI	ACTGG	2 cut(s)	83, 152
BssECI	CCNNGG	1 cut(s)	31
BssMI	GATC	2 cut(s)	243, 278
BssNI	GRCGYC	1 cut(s)	207
BssT1I	CCWWGG	1 cut(s)	31
Bst4CI	ACNGT	2 cut(s)	143, 262
BstACI	GRCGYC	1 cut(s)	207
BstBI	TTCGAA	1 cut(s)	435
BstC8I	GCNNGC	1 cut(s)	117
BstFNI	CGCG	2 cut(s)	41, 314
BstHHI	GCGC	1 cut(s)	115
BstKTI	GATC	2 cut(s)	246, 281
BstMAI	GTCTC	1 cut(s)	251
BstMBI	GATC	2 cut(s)	243, 278
BstMWI	GCNNNNNNNGC	1 cut(s)	215
BstNSI	RCATGY	1 cut(s)	392
BstSFI	CTRYAG	1 cut(s)	343
BstUI	CGCG	2 cut(s)	41, 314
BstX2I	RGATCY	1 cut(s)	278
BstYI	RGATCY	1 cut(s)	278
BsuI	GTATCC	3 cut(s)	187, 281, 302
BtsI	GCAGTG	1 cut(s)	108
BtsIMutI	CAGTG	1 cut(s)	108
Cac8I	GCNNGC	1 cut(s)	117
CfoI	GCGC	1 cut(s)	115
Cfr13I	GGNCC	1 cut(s)	36
CseI	GACGC	1 cut(s)	215
CviAII	CATG	4 cut(s)	16, 47, 137, 389
CviJI	RGCY	6 cut(s)	59, 67, 119, 148, 348, 370
CviKI_1	RGCY	6 cut(s)	59, 67, 119, 148, 348, 370
DpnI	GATC	2 cut(s)	245, 280
DpnII	GATC	2 cut(s)	243, 278
EciI	GGCGGA	1 cut(s)	233
Eco130I	CCWWGG	1 cut(s)	31
Eco32I	GATATC	2 cut(s)	169, 421
Eco47I	GGWCC	1 cut(s)	36
EcoRV	GATATC	2 cut(s)	169, 421
EcoT14I	CCWWGG	1 cut(s)	31
EcoT22I	ATGCAT	1 cut(s)	390
ErhI	CCWWGG	1 cut(s)	31
FaeI	CATG	4 cut(s)	19, 50, 140, 392
FaiI	YATR	9 cut(s)	7, 17, 48, 93, 98, 138, 306, 374, 390
FaqI	GGGAC	1 cut(s)	49
FatI	CATG	4 cut(s)	15, 46, 136, 388
FauI	CCCGC	1 cut(s)	268
FblI	GTMKAC	3 cut(s)	75, 324, 393
Fnu4HI	GCNGC	1 cut(s)	149
Fsp4HI	GCNGC	1 cut(s)	149
FspI	TGCGCA	1 cut(s)	114
GlaI	GCGC	1 cut(s)	114
GluI	GCNGC	1 cut(s)	149
HapII	CCGG	2 cut(s)	60, 378
HgaI	GACGC	1 cut(s)	215
HhaI	GCGC	1 cut(s)	115
Hin1I	GRCGYC	1 cut(s)	207
Hin1II	CATG	4 cut(s)	19, 50, 140, 392
Hin6I	GCGC	1 cut(s)	113
HinP1I	GCGC	1 cut(s)	113
HincII	GTYRAC	1 cut(s)	76
HindII	GTYRAC	1 cut(s)	76
HinfI	GANTC	1 cut(s)	410
HpaII	CCGG	2 cut(s)	60, 378
HphI	GGTGA	1 cut(s)	152
Hpy166II	GTNNAC	3 cut(s)	76, 325, 394
Hpy188I	TCNGA	1 cut(s)	123
Hpy8I	GTNNAC	3 cut(s)	76, 325, 394
HpyAV	CCTTC	3 cut(s)	313, 408, 431
HpyCH4III	ACNGT	2 cut(s)	143, 262
HpyCH4V	TGCA	3 cut(s)	46, 296, 388
HpyF10VI	GCNNNNNNNGC	1 cut(s)	215
Hsp92I	GRCGYC	1 cut(s)	207
Hsp92II	CATG	4 cut(s)	19, 50, 140, 392
HspAI	GCGC	1 cut(s)	113
Kzo9I	GATC	2 cut(s)	243, 278
LmnI	GCTCC	1 cut(s)	124
LpnPI	CCDG	7 cut(s)	64, 73, 165, 176, 223, 312, 391
LweI	GCATC	1 cut(s)	375
MalI	GATC	2 cut(s)	245, 280
MboI	GATC	2 cut(s)	243, 278
MflI	RGATCY	1 cut(s)	278
MluCI	AATT	1 cut(s)	124
MluI	ACGCGT	1 cut(s)	312
MnlI	CCTC	1 cut(s)	81
Mph1103I	ATGCAT	1 cut(s)	390
MslI	CAYNNNNRTG	1 cut(s)	101
MspI	CCGG	2 cut(s)	60, 378
MvnI	CGCG	2 cut(s)	41, 314
MwoI	GCNNNNNNNGC	1 cut(s)	215
NdeII	GATC	2 cut(s)	243, 278
NlaIII	CATG	4 cut(s)	19, 50, 140, 392
NlaIV	GGNNCC	2 cut(s)	29, 37
NsbI	TGCGCA	1 cut(s)	114
NsiI	ATGCAT	1 cut(s)	390
NspI	RCATGY	1 cut(s)	392
NspV	TTCGAA	1 cut(s)	435
PfeI	GAWTC	1 cut(s)	410
PkrI	GCNGC	1 cut(s)	150
PsiI	TTATAA	1 cut(s)	374
PspN4I	GGNNCC	2 cut(s)	29, 37
PspPI	GGNCC	1 cut(s)	36
PsuI	RGATCY	1 cut(s)	278
RseI	CAYNNNNRTG	1 cut(s)	101
SalI	GTCGAC	1 cut(s)	74
SatI	GCNGC	1 cut(s)	149
Sau3AI	GATC	2 cut(s)	243, 278
Sau96I	GGNCC	1 cut(s)	36
SetI	ASST	9 cut(s)	33, 69, 92, 121, 204, 301, 324, 350, 372
SfaNI	GCATC	1 cut(s)	375
SfcI	CTRYAG	1 cut(s)	343
SfuI	TTCGAA	1 cut(s)	435
SinI	GGWCC	1 cut(s)	36
SmiMI	CAYNNNNRTG	1 cut(s)	101
Sse9I	AATT	1 cut(s)	124
SsiI	CCGC	4 cut(s)	39, 149, 218, 275
StyI	CCWWGG	1 cut(s)	31
TaaI	ACNGT	2 cut(s)	143, 262
TaqI	TCGA	5 cut(s)	75, 86, 176, 266, 435
TasI	AATT	1 cut(s)	124
TauI	GCSGC	1 cut(s)	151
TfiI	GAWTC	1 cut(s)	410
TscAI	CASTG	1 cut(s)	108
TspDTI	ATGAA	1 cut(s)	4
TspGWI	ACGGA	1 cut(s)	300
TspRI	CASTG	1 cut(s)	108
VpaK11BI	GGWCC	1 cut(s)	36
XceI	RCATGY	1 cut(s)	392
XmiI	GTMKAC	3 cut(s)	75, 324, 393
Zsp2I	ATGCAT	1 cut(s)	390

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.