RLG00000025841
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0013993
Physical Location & Seq
Reverse (-)
48627482 .. 48630660
3179 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025841

Sequence Viewer

Length: 3060 bp
ATGTCAACCCTTTTGGTGTTCTTCTTCTTCTTTTCCATTGGCCTCTCTCTTAGTTTTGCTGAGGGAGCTCAAACTGTACCTAATGATGAACTATCAGCCTTGCTATCATTCAAAGCTGGCCTAGTTGATCCAATGGATGGCCTGAAGGATTGGAAAATGGCGAGTAACGCGATCGGGGAAGTTGGTTCACTCCATTGTAACTGGAATGGAGTTGTGTGCAACTCCAGAGGCTATGTACAGAAGCTGGACATCTCCAACATGAACATCAGCGGCCTTGTATCAGATCACATTCAAGCCTTAACTAGTGTTTCTGTTTTCAACATTTCTTGCAATGGCTTTACAACATCACTGCCAAAGTTCCTATCCAACCTTACCTCACTGAGCATCATTGATGTGAGTGACAACAACTTTGTAGGTGAGTTCCCAATAGGGCTAGGAAGAGCTTCAGGGCTGATTTCAGTCAACGCATCGAGCAACAACTTTTCGGGGTTTCTTCCAGAGGATCTTGGTAACGCCACTTCCCTTGAGATCCTGGACTTCAGAGGGAGCTACTTTGAAGGTTCAGTTCCAACATCATACAAGAACTTGCAGAAGCTGAAGTTTCTAGGCCTTTCTGGTAACAATCTCACCGGAAACATTCCAAAAGAACTTGGGCAGCTTTCTTCTTTGGAGACTATTATTCTTGGATACAATGAGTTTGTTGGTGAAATCCCGGCGGAGTTTGGCAATCTTACCAATCTTCAGTATCTTGACTTGGCAGTTGGCAGCCTCAGTGGTCAAATCCCACCTGAATTGGGTAGGCTACAAAAACTAACCACAGTTTATTTGTACAAAAACAACTTCACAGGAAAAATCCCACCAGAGATAGGTAACATAACATCTCTAGTGTACCTGGATCTCTCCGATAATCAGATTTCGGGTGAGATTCCAGCTGAGCTTGCAGGGTTGACAGATTTGCAGCTTCTGAACTTAATGTGCAATAGGCTAACAGGTTTGGTACCTACCAAGCTTGGGGATTTGACCAAGTTAGCTATACTTGAGCTATGGAAGAATTCTCTGACAGGTCCATTGCCACTGAACCTTGGCAAGAATTCTCCATTGCAATGGTTAGATGTGTCTTCAAATTTCTTATCCGGCGATATCCCACCAGGTTTGTGTGATTCTGGTAATCTCACTAAGCTCATTCTGTTCAACAACTCCTTCTCTGGTTCTATTCCATTAGGCCTTTCAACTTGTTTGTCATTGGTCCGTGTTCGGATGCAAAACAATCTCATTTCAGGAACTATCCCAGTTGGCCTGGGAACACTTCCTAATCTTCAAAGGCTAGAATTGGCTAAAAACAATCTTACTGGCCAAATTCCTCAGGACATTGCTTTGTCTACATCACTTTCTTTCTTTGATGTTTCTTGGAACCACATTGAATCTTCTATTCCTTCTAGCATTCTCTCCATTCCAAATCTCCAAACCTTCATGGCTTCCAGCAACAACTTGCAAGGCAAACTCCCAGACCAGTTCCAAGATTGCCCGTCGCTGTCAGTGCTTGATATATCAAGCAACCATTTCTCTGGAAACATTCCAGAGAGTATAGCTTCTTGCGAGAAGCTAGTGAGTCTGAGCCTTAGAAGTAACCAGTTCAGTGGTGAGATCCCTAGAGCTATCGCAACAATGCCTACATTATCCATTCTTGATCTGTCCAACAATTCTTTGATAGGTAGAATCCCTGAAAGCTTTGGAAGCTCACCAGCCTTGGAGATGCTTAACTTGTCTTTCAACAGACTTGAGGGTCCAGTGCCGGCAAATGGTATCTTAATGACCATAAATCCCAATGATCTTGTAGGCAATGCCGGTCTCTGTGGAGGCATACTTCCACCTTGTGCTCATAGTTTAGTTGCCACAGCAAGCCGGCATCAGAGCATGCACATCAAACACATCATTACTGGATTCATCATTGGAATCGGAGTCATCTCAACTCTCGGCGTCACACTTTTCACTGGGAGATGGGTATATAGGAAGTGGTACTGGTATAGCAGCTCTGACGGTTGGTTCAAGAACAGCAGTCAAGAATGGCCTTGGAGGCTAGTAGCATTTCAGAGAATCAACTTCACAAGTAGTGACATTCTTGCTTGCATAAAGGAATCAAATGTCATTGGCATGGGTGGGGCTGGTATTGTTTACAAGGCTGAAGTCCATCGGCCGCATTCAGTTGTGGCAGTTAAGAAGCTATGGAGGCCAGGGACAGACATTGAAAATGGGGAGGACCTATTTGCTGAAGTGAATCTCCTTGGCAGGCTCCGGCATAGGAACATTGTCCGGTTATTAGGGTACCTCCACAATGAAACTGATGTCATTATGGTATCCGAGTACATGCCAAATGGGAACTTAGGTACTGCATTGCATGGTGATCAAGCAGGAAAATTGCTGGTTGATTGGGTTTCAAGGTACAACATAGCAGTTGGGGTAGCACAAGGTCTAAATTATCTTCACCATGACTGTCAGCCACCAGTCATTCACCGAGATATCAAATCAAACAACATTCTGCTCGATAAAAATCTTGACGCCAGAATAGCAGATTTCGGGTTGGCAAGGATGATGATGCACAAGAATGAGACAGTTTCAATCGTGGCAGGGTCTTATGGATACATTGCACCTGAATACGGATATGCCATGAAGGTTGATGAAAAGACTGATATCTATAGCTATGGTGTAGTTCTTTTAGAGCTTCTAACTGGAAAAATGCCAGTAGACCCTGCTTTCGGGGAATCTGTGGATGTTGTGGAATTTGTTCGAAAGAAGATGCGAAACAAAAGAGCGTTAGAAGAAGCGCTAGACTCCAGCATCGCCGGAGAATGCAAGCATGTCCAGGAAGAAATGCTTCTTGTTCTCAGAATCGCACTACTTTGTACCGCAAAGCTACCGAAAGACAGGCCATCAATGAGAGATATAATCACAATGCTGGGAGAGGCAAAGCCAAGGAGGAAGAGCATTTCTGAGAATTGGGACCAAAAGACTCGCAAGGAGAAGCCAATCTTTAGCACCTCACCAGTCATAGGACTTATTTAG

Protein Analysis

1020

Amino Acids

111.2

Weight (kDa)

5.96

Isoelectric Point (pI)

37.26

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 74 8.5e-08 Leucine rich repeat N-terminal domain
LRR_14 PF23598 168 - 272 6.3e-09 Leucine-rich repeat region
LRR_14 PF23598 241 - 447 1.5e-13 Leucine-rich repeat region
LRR_8 PF13855 485 - 545 2e-07 Leucine rich repeat
LRR_8 PF13855 534 - 593 7.4e-09 Leucine rich repeat
Pkinase PF00069 712 - 981 5.7e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 712 - 985 1.2e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013993)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08590
fragaria_vesca FvH4_6g01540 FvH4_6g20240
malus_domestica MD00G1149400.v1.1 MD04G1232500.v1.1
prunus_persica Prupe.6G352100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0449301
rosa_laevigata RLG00000025841
rosa_multiflora Rmu_sc0007719.1_g000004 Rmu_sc0018234.1_g000001
rosa_roxburghii Rroxscaffold_6G00427300
rosa_rugosa Rorug02G0619500
rosa_samantha Rh3AG017500 Rh3BG017600 Rh3CG016600 Rh3DG017900
rosa_wichuraiana Rw3G001340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1782
Acc65I GGTACC 2 cut(s) 997, 2322
AccB1I GGYRCC 2 cut(s) 997, 2322
AccB7I CCANNNNNTGG 1 cut(s) 137
AccI GTMKAC 2 cut(s) 1379, 2742
AccII CGCG 1 cut(s) 170
AciI CCGC 4 cut(s) 270, 716, 2195, 2904
AclWI GGATC 5 cut(s) 122, 510, 523, 903, 1639
AcoI YGGCCR 2 cut(s) 1351, 2192
AcsI RAATTY 5 cut(s) 1051, 1090, 1123, 1356, 2777
AcuI CTGAAG 7 cut(s) 164, 429, 523, 617, 725, 2202, 2289
AcyI GRCGYC 2 cut(s) 1977, 2556
AdeI CACNNNGTG 1 cut(s) 1874
AfeI AGCGCT 1 cut(s) 2823
AfiI CCNNNNNNNGG 8 cut(s) 137, 794, 866, 1011, 1799, 2654, 2753, 3047
AhlI ACTAGT 1 cut(s) 302
AjnI CCWGG 6 cut(s) 531, 891, 1147, 1296, 2230, 2859
Alw21I GWGCWC 2 cut(s) 70, 1879
Alw26I GTCTC 3 cut(s) 665, 1853, 2600
AlwI GGATC 5 cut(s) 122, 510, 523, 903, 1639
AlwNI CAGNNNCTG 3 cut(s) 244, 595, 964
Aor51HI AGCGCT 1 cut(s) 2823
ApeKI GCWGC 4 cut(s) 655, 765, 958, 2028
ApoI RAATTY 5 cut(s) 1051, 1090, 1123, 1356, 2777
ArsI GACNNNNNNTTYG 4 cut(s) 392, 424, 2735, 2767
Asp700I GAANNNNTTC 3 cut(s) 442, 2781, 2871
Asp718I GGTACC 2 cut(s) 997, 2322
AspLEI GCGC 1 cut(s) 2824
AspS9I GGNCC 5 cut(s) 1064, 1246, 1784, 2257, 2998
AsuC2I CCSGG 1 cut(s) 713
AsuII TTCGAA 1 cut(s) 2785
AvaII GGWCC 5 cut(s) 1064, 1246, 1784, 2257, 2998
AxyI CCTNAGG 1 cut(s) 1362
BalI TGGCCA 1 cut(s) 1353
BanI GGYRCC 2 cut(s) 997, 2322
BanII GRGCYC 1 cut(s) 70
BarI GAAGNNNNNNTAC 2 cut(s) 502, 534
BbsI GAAGAC 1 cut(s) 1110
Bbv12I GWGCWC 2 cut(s) 70, 1879
BbvCI CCTCAGC 1 cut(s) 60
BbvI GCAGC 4 cut(s) 667, 777, 970, 2040
BccI CCATC 4 cut(s) 131, 1992, 2196, 2935
BciT130I CCWGG 6 cut(s) 533, 893, 1149, 1298, 2232, 2861
BciVI GTATCC 3 cut(s) 680, 2365, 2630
BclI TGATCA 1 cut(s) 2401
BcnI CCSGG 1 cut(s) 713
BcoDI GTCTC 3 cut(s) 665, 1853, 2600
BcuI ACTAGT 1 cut(s) 302
BfmI CTRYAG 1 cut(s) 2692
BfoI RGCGCY 1 cut(s) 2825
BfuI GTATCC 3 cut(s) 680, 2365, 2630
BglI GCCNNNNNGGC 1 cut(s) 2074
BisI GCNGC 6 cut(s) 271, 656, 766, 959, 2029, 2195
BlpI GCTNAGC 1 cut(s) 933
BlsI GCNGC 6 cut(s) 272, 657, 767, 960, 2030, 2196
Bme1390I CCNGG 7 cut(s) 533, 713, 893, 1149, 1298, 2232, 2861
Bme18I GGWCC 5 cut(s) 1064, 1246, 1784, 2257, 2998
BmgT120I GGNCC 5 cut(s) 1064, 1246, 1784, 2257, 2998
BmiI GGNNCC 6 cut(s) 999, 1412, 1785, 2291, 2324, 2999
BmrFI CCNGG 7 cut(s) 533, 713, 893, 1149, 1298, 2232, 2861
BmrI ACTGGG 2 cut(s) 1283, 2001
BmsI GCATC 8 cut(s) 393, 476, 1248, 1743, 1915, 2583, 2784, 2844
BmuI ACTGGG 2 cut(s) 1283, 2001
BpiI GAAGAC 1 cut(s) 1110
BpmI CTGGAG 2 cut(s) 208, 2815
Bpu10I CCTNAGC 1 cut(s) 60
Bpu1102I GCTNAGC 1 cut(s) 933
Bpu14I TTCGAA 1 cut(s) 2785
BpuEI CTTGAG 3 cut(s) 545, 1058, 1799
BpuMI CCSGG 1 cut(s) 713
BsaBI GATNNNNATC 1 cut(s) 2547
BsaHI GRCGYC 2 cut(s) 1977, 2556
BsaI GGTCTC 1 cut(s) 1853
BsaJI CCNNGG 7 cut(s) 1081, 1297, 1746, 2069, 2231, 2281, 2969
BsaWI WCCGGW 2 cut(s) 629, 2310
Bsc4I CCNNNNNNNGG 8 cut(s) 137, 794, 866, 1011, 1799, 2654, 2753, 3047
Bse118I RCCGGY 3 cut(s) 1792, 1844, 1902
Bse21I CCTNAGG 1 cut(s) 1362
Bse3DI GCAATG 8 cut(s) 337, 1067, 1097, 1109, 1368, 1846, 2390, 2640
Bse8I GATNNNNATC 1 cut(s) 2547
BseBI CCWGG 6 cut(s) 533, 893, 1149, 1298, 2232, 2861
BseDI CCNNGG 7 cut(s) 1081, 1297, 1746, 2069, 2231, 2281, 2969
BseGI GGATG 4 cut(s) 142, 1263, 2592, 2773
BseJI GATNNNNATC 1 cut(s) 2547
BseLI CCNNNNNNNGG 8 cut(s) 137, 794, 866, 1011, 1799, 2654, 2753, 3047
BseMI GCAATG 8 cut(s) 337, 1067, 1097, 1109, 1368, 1846, 2390, 2640
BseMII CTCAG 8 cut(s) 51, 371, 784, 924, 1376, 1604, 2896, 2979
BseX3I CGGCCG 1 cut(s) 2192
BseXI GCAGC 4 cut(s) 667, 777, 970, 2040
BseYI CCCAGC 1 cut(s) 2953
Bsh1236I CGCG 1 cut(s) 170
Bsh1285I CGRYCG 2 cut(s) 174, 2195
BshNI GGYRCC 2 cut(s) 997, 2322
BsiEI CGRYCG 2 cut(s) 174, 2195
BsiHKAI GWGCWC 2 cut(s) 70, 1879
BsiSI CCGG 9 cut(s) 630, 713, 1134, 1793, 1845, 1903, 2293, 2311, 2841
BslFI GGGAC 2 cut(s) 2248, 3011
BslI CCNNNNNNNGG 8 cut(s) 137, 794, 866, 1011, 1799, 2654, 2753, 3047
BsmAI GTCTC 3 cut(s) 665, 1853, 2600
BsmFI GGGAC 2 cut(s) 2248, 3011
BsmI GAATGC 3 cut(s) 1440, 2197, 2852
Bso31I GGTCTC 1 cut(s) 1853
Bsp119I TTCGAA 1 cut(s) 2785
Bsp1286I GDGCHC 2 cut(s) 70, 1879
Bsp1407I TGTACA 2 cut(s) 235, 828
Bsp1720I GCTNAGC 1 cut(s) 933
BspACI CCGC 4 cut(s) 270, 716, 2195, 2904
BspCNI CTCAG 8 cut(s) 52, 372, 783, 925, 1375, 1605, 2895, 2980
BspFNI CGCG 1 cut(s) 170
BspLI GGNNCC 6 cut(s) 999, 1412, 1785, 2291, 2324, 2999
BspPI GGATC 5 cut(s) 122, 510, 523, 903, 1639
BspQI GCTCTTC 2 cut(s) 433, 2972
BspT104I TTCGAA 1 cut(s) 2785
BspT107I GGYRCC 2 cut(s) 997, 2322
BspTNI GGTCTC 1 cut(s) 1853
BsrDI GCAATG 8 cut(s) 337, 1067, 1097, 1109, 1368, 1846, 2390, 2640
BsrFI RCCGGY 3 cut(s) 1792, 1844, 1902
BsrGI TGTACA 2 cut(s) 235, 828
BssAI RCCGGY 3 cut(s) 1792, 1844, 1902
BssECI CCNNGG 7 cut(s) 1081, 1297, 1746, 2069, 2231, 2281, 2969
BssNI GRCGYC 2 cut(s) 1977, 2556
BssT1I CCWWGG 5 cut(s) 1081, 1746, 2069, 2281, 2969
Bst2UI CCWGG 6 cut(s) 533, 893, 1149, 1298, 2232, 2861
Bst4CI ACNGT 5 cut(s) 76, 820, 2039, 2492, 2611
Bst6I CTCTTC 2 cut(s) 433, 2972
BstACI GRCGYC 2 cut(s) 1977, 2556
BstAUI TGTACA 2 cut(s) 235, 828
BstBI TTCGAA 1 cut(s) 2785
BstC8I GCNNGC 9 cut(s) 118, 939, 1794, 1900, 1904, 1916, 2125, 2288, 2852
BstF5I GGATG 4 cut(s) 142, 1263, 2592, 2773
BstFNI CGCG 1 cut(s) 170
BstH2I RGCGCY 1 cut(s) 2825
BstHHI GCGC 1 cut(s) 2824
BstMAI GTCTC 3 cut(s) 665, 1853, 2600
BstMCI CGRYCG 2 cut(s) 174, 2195
BstMWI GCNNNNNNNGC 8 cut(s) 65, 167, 938, 1537, 1734, 2074, 2227, 2564
BstNI CCWGG 6 cut(s) 533, 893, 1149, 1298, 2232, 2861
BstNSI RCATGY 3 cut(s) 1918, 2368, 2858
BstSCI CCNGG 7 cut(s) 531, 711, 891, 1147, 1296, 2230, 2859
BstSFI CTRYAG 1 cut(s) 2692
BstUI CGCG 1 cut(s) 170
BstV1I GCAGC 4 cut(s) 667, 777, 970, 2040
BstV2I GAAGAC 1 cut(s) 1110
BstX2I RGATCY 4 cut(s) 502, 528, 895, 1644
BstXI CCANNNNNNTGG 3 cut(s) 1104, 1565, 1637
BstYI RGATCY 4 cut(s) 502, 528, 895, 1644
BstZI CGGCCG 1 cut(s) 2192
Bsu36I CCTNAGG 1 cut(s) 1362
BsuI GTATCC 3 cut(s) 680, 2365, 2630
BtgZI GCGATG 1 cut(s) 2821
BtsCI GGATG 4 cut(s) 142, 1263, 2592, 2773
BtsI GCAGTG 1 cut(s) 347
BtsIMutI CAGTG 8 cut(s) 347, 377, 778, 1073, 1542, 1642, 1794, 1989
Cac8I GCNNGC 9 cut(s) 118, 939, 1794, 1900, 1904, 1916, 2125, 2288, 2852
CaiI CAGNNNCTG 3 cut(s) 244, 595, 964
CfoI GCGC 1 cut(s) 2824
Cfr10I RCCGGY 3 cut(s) 1792, 1844, 1902
Cfr13I GGNCC 5 cut(s) 1064, 1246, 1784, 2257, 2998
CseI GACGC 2 cut(s) 1966, 2564
CsiI ACCWGGT 1 cut(s) 1147
CspCI CAANNNNNGTGG 6 cut(s) 505, 540, 774, 809, 1134, 1169
CviAII CATG 9 cut(s) 259, 1471, 1915, 2152, 2365, 2396, 2486, 2665, 2855
DraIII CACNNNGTG 1 cut(s) 1874
DrdI GACNNNNNNGTC 1 cut(s) 1782
DseDI GACNNNNNNGTC 1 cut(s) 1782
EaeI YGGCCR 2 cut(s) 1351, 2192
EagI CGGCCG 1 cut(s) 2192
Eam1104I CTCTTC 2 cut(s) 433, 2972
EarI CTCTTC 2 cut(s) 433, 2972
EciI GGCGGA 1 cut(s) 731
Ecl136II GAGCTC 1 cut(s) 68
EclXI CGGCCG 1 cut(s) 2192
Eco130I CCWWGG 5 cut(s) 1081, 1746, 2069, 2281, 2969
Eco147I AGGCCT 2 cut(s) 609, 1224
Eco24I GRGCYC 1 cut(s) 70
Eco31I GGTCTC 1 cut(s) 1853
Eco32I GATATC 3 cut(s) 1141, 2518, 2689
Eco47I GGWCC 5 cut(s) 1064, 1246, 1784, 2257, 2998
Eco47III AGCGCT 1 cut(s) 2823
Eco52I CGGCCG 1 cut(s) 2192
Eco53kI GAGCTC 1 cut(s) 68
Eco57I CTGAAG 7 cut(s) 164, 429, 523, 617, 725, 2202, 2289
Eco81I CCTNAGG 1 cut(s) 1362
EcoICRI GAGCTC 1 cut(s) 68
EcoO109I RGGNCCY 1 cut(s) 2257
EcoRI GAATTC 2 cut(s) 1051, 1090
EcoRII CCWGG 6 cut(s) 531, 891, 1147, 1296, 2230, 2859
EcoRV GATATC 3 cut(s) 1141, 2518, 2689
EcoT14I CCWWGG 5 cut(s) 1081, 1746, 2069, 2281, 2969
EcoT38I GRGCYC 1 cut(s) 70
ErhI CCWWGG 5 cut(s) 1081, 1746, 2069, 2281, 2969
FaeI CATG 9 cut(s) 262, 1474, 1918, 2155, 2368, 2399, 2489, 2668, 2858
FalI AAGNNNNNCTT 4 cut(s) 2856, 2888, 3011, 3043
FaqI GGGAC 2 cut(s) 2248, 3011
FatI CATG 9 cut(s) 258, 1470, 1914, 2151, 2364, 2395, 2485, 2664, 2854
FbaI TGATCA 1 cut(s) 2401
FblI GTMKAC 2 cut(s) 1379, 2742
Fnu4HI GCNGC 6 cut(s) 271, 656, 766, 959, 2029, 2195
FokI GGATG 4 cut(s) 149, 1270, 2599, 2780
FriOI GRGCYC 1 cut(s) 70
Fsp4HI GCNGC 6 cut(s) 271, 656, 766, 959, 2029, 2195
GlaI GCGC 1 cut(s) 2823
GluI GCNGC 6 cut(s) 271, 656, 766, 959, 2029, 2195
GsaI CCCAGC 1 cut(s) 2957
GsuI CTGGAG 2 cut(s) 208, 2815
HaeII RGCGCY 1 cut(s) 2825
HapII CCGG 9 cut(s) 630, 713, 1134, 1793, 1845, 1903, 2293, 2311, 2841
HgaI GACGC 2 cut(s) 1966, 2564
HhaI GCGC 1 cut(s) 2824
Hin1I GRCGYC 2 cut(s) 1977, 2556
Hin1II CATG 9 cut(s) 262, 1474, 1918, 2155, 2368, 2399, 2489, 2668, 2858
Hin6I GCGC 1 cut(s) 2822
HinP1I GCGC 1 cut(s) 2822
HincII GTYRAC 3 cut(s) 6, 463, 948
HindII GTYRAC 3 cut(s) 6, 463, 948
HindIII AAGCTT 2 cut(s) 1007, 1726
HpaII CCGG 9 cut(s) 630, 713, 1134, 1793, 1845, 1903, 2293, 2311, 2841
Hpy166II GTNNAC 8 cut(s) 6, 188, 463, 889, 948, 1380, 2173, 2743
Hpy8I GTNNAC 8 cut(s) 6, 188, 463, 889, 948, 1380, 2173, 2743
Hpy99I CGWCG 1 cut(s) 1531
HpyAV CCTTC 6 cut(s) 139, 551, 1210, 1443, 1477, 2662
HpyCH4III ACNGT 5 cut(s) 76, 820, 2039, 2492, 2611
HpyF10VI GCNNNNNNNGC 8 cut(s) 65, 167, 938, 1537, 1734, 2074, 2227, 2564
Hsp92I GRCGYC 2 cut(s) 1977, 2556
Hsp92II CATG 9 cut(s) 262, 1474, 1918, 2155, 2368, 2399, 2489, 2668, 2858
HspAI GCGC 1 cut(s) 2822
KpnI GGTACC 2 cut(s) 1001, 2326
KroI GCCGGC 2 cut(s) 1792, 1902
KroNI GCCGGC 2 cut(s) 1794, 1904
Ksp22I TGATCA 1 cut(s) 2401
LguI GCTCTTC 2 cut(s) 433, 2972
LmnI GCTCC 3 cut(s) 65, 546, 2295
Lsp1109I GCAGC 4 cut(s) 667, 777, 970, 2040
LweI GCATC 8 cut(s) 393, 476, 1248, 1743, 1915, 2583, 2784, 2844
MabI ACCWGGT 1 cut(s) 1147
MaeIII GTNAC 9 cut(s) 164, 197, 398, 509, 617, 869, 1625, 1978, 2111
MflI RGATCY 4 cut(s) 502, 528, 895, 1644
MhlI GDGCHC 2 cut(s) 70, 1879
MlsI TGGCCA 1 cut(s) 1353
MluNI TGGCCA 1 cut(s) 1353
MlyI GAGTC 4 cut(s) 1618, 1968, 2822, 3001
MmeI TCCRAC 4 cut(s) 279, 390, 593, 1719
Mox20I TGGCCA 1 cut(s) 1353
MroNI GCCGGC 2 cut(s) 1792, 1902
MroXI GAANNNNTTC 3 cut(s) 442, 2781, 2871
MscI TGGCCA 1 cut(s) 1353
MseI TTAA 5 cut(s) 299, 971, 1758, 1808, 2214
MslI CAYNNNNRTG 4 cut(s) 392, 1102, 2150, 2601
Msp20I TGGCCA 1 cut(s) 1353
MspA1I CMGCKG 2 cut(s) 270, 932
MspI CCGG 9 cut(s) 630, 713, 1134, 1793, 1845, 1903, 2293, 2311, 2841
MspR9I CCNGG 7 cut(s) 533, 713, 893, 1149, 1298, 2232, 2861
Mva1269I GAATGC 3 cut(s) 1440, 2197, 2852
MvaI CCWGG 6 cut(s) 533, 893, 1149, 1298, 2232, 2861
MvnI CGCG 1 cut(s) 170
MwoI GCNNNNNNNGC 8 cut(s) 65, 167, 938, 1537, 1734, 2074, 2227, 2564
NaeI GCCGGC 2 cut(s) 1794, 1904
NciI CCSGG 1 cut(s) 713
NgoMIV GCCGGC 2 cut(s) 1792, 1902
NlaIII CATG 9 cut(s) 262, 1474, 1918, 2155, 2368, 2399, 2489, 2668, 2858
NlaIV GGNNCC 6 cut(s) 999, 1412, 1785, 2291, 2324, 2999
NmeAIII GCCGAG 1 cut(s) 1953
NmuCI GTSAC 3 cut(s) 398, 1978, 2111
NspI RCATGY 3 cut(s) 1918, 2368, 2858
NspV TTCGAA 1 cut(s) 2785
PaeI GCATGC 1 cut(s) 1918
PceI AGGCCT 2 cut(s) 609, 1224
PciSI GCTCTTC 2 cut(s) 433, 2972
PctI GAATGC 3 cut(s) 1440, 2197, 2852
PdiI GCCGGC 2 cut(s) 1794, 1904
PdmI GAANNNNTTC 3 cut(s) 442, 2781, 2871
PflMI CCANNNNNTGG 1 cut(s) 137
PfoI TCCNGGA 2 cut(s) 531, 2859
PkrI GCNGC 6 cut(s) 272, 657, 767, 960, 2030, 2196
Ple19I CGATCG 1 cut(s) 174
PleI GAGTC 4 cut(s) 1617, 1967, 2822, 3001
PpsI GAGTC 4 cut(s) 1617, 1967, 2822, 3001
PpuMI RGGWCCY 1 cut(s) 2257
Psp124BI GAGCTC 1 cut(s) 70
Psp5II RGGWCCY 1 cut(s) 2257
Psp6I CCWGG 6 cut(s) 531, 891, 1147, 1296, 2230, 2859
PspFI CCCAGC 1 cut(s) 2953
PspGI CCWGG 6 cut(s) 531, 891, 1147, 1296, 2230, 2859
PspN4I GGNNCC 6 cut(s) 999, 1412, 1785, 2291, 2324, 2999
PspPI GGNCC 5 cut(s) 1064, 1246, 1784, 2257, 2998
PspPPI RGGWCCY 1 cut(s) 2257
PstNI CAGNNNCTG 3 cut(s) 244, 595, 964
PsuI RGATCY 4 cut(s) 502, 528, 895, 1644
PvuI CGATCG 1 cut(s) 174
PvuII CAGCTG 1 cut(s) 932
RseI CAYNNNNRTG 4 cut(s) 392, 1102, 2150, 2601
SacI GAGCTC 1 cut(s) 70
SapI GCTCTTC 2 cut(s) 433, 2972
SaqAI TTAA 5 cut(s) 299, 971, 1758, 1808, 2214
SatI GCNGC 6 cut(s) 271, 656, 766, 959, 2029, 2195
Sau96I GGNCC 5 cut(s) 1064, 1246, 1784, 2257, 2998
SchI GAGTC 4 cut(s) 1618, 1968, 2822, 3001
ScrFI CCNGG 7 cut(s) 533, 713, 893, 1149, 1298, 2232, 2861
SduI GDGCHC 2 cut(s) 70, 1879
SexAI ACCWGGT 1 cut(s) 1147
SfaNI GCATC 8 cut(s) 393, 476, 1248, 1743, 1915, 2583, 2784, 2844
SfcI CTRYAG 1 cut(s) 2692
SfuI TTCGAA 1 cut(s) 2785
SinI GGWCC 5 cut(s) 1064, 1246, 1784, 2257, 2998
SmiMI CAYNNNNRTG 4 cut(s) 392, 1102, 2150, 2601
SmlI CTYRAG 3 cut(s) 524, 1037, 1778
SmoI CTYRAG 3 cut(s) 524, 1037, 1778
SpeI ACTAGT 1 cut(s) 302
SphI GCATGC 1 cut(s) 1918
SseBI AGGCCT 2 cut(s) 609, 1224
SsiI CCGC 4 cut(s) 270, 716, 2195, 2904
SstI GAGCTC 1 cut(s) 70
StuI AGGCCT 2 cut(s) 609, 1224
StyD4I CCNGG 7 cut(s) 531, 711, 891, 1147, 1296, 2230, 2859
StyI CCWWGG 5 cut(s) 1081, 1746, 2069, 2281, 2969
TaaI ACNGT 5 cut(s) 76, 820, 2039, 2492, 2611
TaqI TCGA 3 cut(s) 470, 2541, 2785
TatI WGTACW 3 cut(s) 235, 828, 2361
TauI GCSGC 2 cut(s) 273, 2197
Tru1I TTAA 5 cut(s) 299, 971, 1758, 1808, 2214
Tru9I TTAA 5 cut(s) 299, 971, 1758, 1808, 2214
TscAI CASTG 8 cut(s) 354, 384, 778, 1080, 1542, 1642, 1794, 1996
TseFI GTSAC 3 cut(s) 398, 1978, 2111
TseI GCWGC 4 cut(s) 655, 765, 958, 2028
Tsp45I GTSAC 3 cut(s) 398, 1978, 2111
TspDTI ATGAA 7 cut(s) 102, 275, 1459, 1933, 2349, 2681, 2691
TspGWI ACGGA 2 cut(s) 1238, 2670
TspRI CASTG 8 cut(s) 354, 384, 778, 1080, 1542, 1642, 1794, 1996
Van91I CCANNNNNTGG 1 cut(s) 137
VpaK11BI GGWCC 5 cut(s) 1064, 1246, 1784, 2257, 2998
XapI RAATTY 5 cut(s) 1051, 1090, 1123, 1356, 2777
XceI RCATGY 3 cut(s) 1918, 2368, 2858
XmiI GTMKAC 2 cut(s) 1379, 2742
XmnI GAANNNNTTC 3 cut(s) 442, 2781, 2871
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.