Rroxscaffold_6G00427300
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0013993
Physical Location & Seq
Forward (+)
47903858 .. 47907198
3341 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00427300.1

Sequence Viewer

Length: 3057 bp
ATGTCAACCCTTTTGGTGTTCTACTTCTTTTCCATTGGCCTCTCTCTTATTTTTGCTGAGGGAGCTCAAACTGTACCTAATGATGAACTATCAACCTTGCTGTCATTCAAAGCTGGCCTAGTTGATCCAATGGATGGCCTGAAGGATTGGAAAATGCCGAGCAACGAGATTGGGGAAGTTGGTTCACTCCATTGCAACTGGACTGGAGTTATGTGCAACTCCAGAGGCTATGTACAGAAGCTGGACATCTCCAACATGAACATCAGCGGCCTTGTATCAGATCACATTCAAGCCTTAACTAGTCTTTCTGTTTTCAACATTTCTTGCAATGGCTTTACAACTTCACTGCCAAAGTCCCTATCCAACCTTACCTCACTGAGCATCATTGATGTGAGTGACAACTACTTTGTTGGTGAGTTCCCAATAGGGCTAGGAAGAGCTTCAGGGCTGATTTCAGTCAACGCATCGAGCAACAACTTTTCGGGGTTTCTTCCAGAGGATCTTAGCAATGCCACTTCCCTTGAGATCCTGGACTTCAGAGGGAGCTACTTTGAAGGTTCAGTTCCAACATCATACAAGAACTTGCAGAAGCTGAAGTTTCTAGGCCTTTCTGGTAACAATCTCACCGGAAACATTCCAAAAGAACTTGGGCAGCTTTCTTCTTTGGAGACTATTATTCTTGGATACAATGAGTTTGTTGGTGAAATCCCCGCGGAGTTTGGCAATCTCACCAATCTTCAGTATCTTGACTTAGCAGTTGGAAGTCTCAGTGGTCAAATCCCACCTGAATTGGGTAGGCTACAAAAACTAACCACAGTTTATTTGTACAAAAACAACTTCACAGGAAAAATCCCACCAGAGATTGGTAACATTACATCTCTAGTGTACCTGGATCTCTCCGATAATAAGATTTTGGGTGAGATTCCAGCTGAGCTTGCAGGGTTGACAAATTTGCAGCTTCTGAACTTAATGTGCAATAGGCTAACAGGTTTGGTACCTACCAAGCTTGGGGATTTAACCAAGTTAGCTATACTTGAGCTGTGGAAGAATTCTCTGACAGGTCCATTGCCACTGAACCTTGGCAAGAATTCTCCATTGCAATGGCTAGATGTGTCTTCAAATTTCTTATCCGGCGATATCCCACCAGGTTTGTGTGATTCTGGTAATCTCACTAAGCTCATTCTGTTCAACAACTCCTTCTCTGGTTCTATTCCATTAGGCCTTTCAACTTGTTTGTCATTGGTCCGTGTTCGGATGCAAAACAATCTCATTTCAGGAACTATCCCAGTTGGCCTGGGAACACTTCCTAGTCTTCAAAGGCTAGAATTGGCTAAAAACAATCTTACTGGCCAAATTCCTCAGGACATTGCTTTGTCTACATCACTTTCTTTCATTGATGTCTCTTGGAACCACATTGAATCTTCTATTCCCTCTAGCATTCTCTCCATTCCAAATCTCCAAACCTTCATGTCTTCCAACAACAACTTGCAAGGCAAGCTCCCAGACCAGTTCCAAGATTGCCCGTCGCTGTCAGTGCTTGATATATCAAGCAACCATTTCTCTGGAAACATTCCAGAGAGTATAGCTTCTTGCGAGAAGCTAGTGAGTCTGAGCCTTAGAAGTAACCAGTTCAGTGGTGAGATTCCTAGAGCTATCGCAACAATGCCTACATTATCCATTCTTGATCTGTCCAACAATTCTTTGATTGGTAGAATCCCTGAAAGCTTTGGAAGCTCACCAGCCTTGGAGATGCTTAACTTGTCTTTCAACAGACTTGAGGGTCCAGTGCCGGCAAATGGTATCTTAATGACCATAAATCCCAATGATCTTGTTGGCAATGCCGGTCTCTGTGGAGGCATACTTCCACCTTGTGCTCATAGTTTAGCTGCCACAGCAAGCCGGCATCAGAGCATGCACATCAAACACATCATTACTGGATTCGTCATTGGAATCGGAGTCATCTCAACTATCGGTGTCACCCTTTTCACTGGGAGATGGGTATATAGGAAGTGGTACTTGTATAGCAGCTCTGACAGTTGGTTCAAGAACAGCAGTCAAGAATGGCCTTGGAGGCTAGTAGCATTTCAGAGAATCAACTTCACAAGTAGTGACATTCTTGCTTGCATAAAGGAATCAAATGTCATTGGCATGGGTGGGGCTGGTATTGTTTACAAGGCTGAAGTCCATCGGCCGCATTCAGTTGTGGCAGTTAAGAAGCTATGGAGGCCAGGGACAGATATTGAAAATGGGGAGGACCTATTTGCTGAAGTGAATCTCCTTGGCAGGCTCCGGCATAGGAACATTGTCCGGTTATTAGGGTACCTCCACAATGAAACTGATGTCATTATGGTATCCGAGTACATGCCAAATGGGAACTTAGGTACTGCATTGCATGGAGATCAAGCAGGAAAATTGCTGGTTGATTGGGTTTCAAGGTACAACATAGCAGTTGGGGTAGCACAAGGTCTAAATTATCTTCACCATGACTGTCAGCCACCAGTCATTCACCGAGATATCAAATCAAACAACATTCTGCTCGATAAAAATCTTGACGCCAGAATAGCAGATTTCGGGTTGGCAAGGATGATGATGCACAAGAATGAGACAGTTTCAATCGTGGCAGGGTCTTATGGATACATTGCACCTGAATACGGATACGCCATGAAGGTTGATGAAAAGACTGATATCTATAGCTATGGTGTTGTTCTTTTAGAGCTTCTAACTGGAAAAATGCCGGTAGACCCTGCTTTTGGGGAATCTGTGGATGTCGTGGAATTTGTTCGAAAGAAGATGCGAAACAAAAGAGCTTTAGAAGAAGCGCTAGACTCCAGCATCGCCGGAGAATGCAAGCATGTCCAGGAAGAAATGCTTCTTGTTCTCAGAATCGCACTACTTTGTACTGCAAAGCTACCGAAAGACAGACCATCAATGAGAGACATAATCACAATGCTGGGAGAGGCAAAACCAAGGAGGAAAAGCATTTCTGAGAATTGGGACCAAAAGACTCGCAAGGAGAAGCCAATCTTTAGCACCTCACCAGTCATAGGACTTATTTAG

Protein Analysis

1018

Amino Acids

111.19

Weight (kDa)

6.03

Isoelectric Point (pI)

39.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 27 - 73 7.3e-08 Leucine rich repeat N-terminal domain
LRR_8 PF13855 78 - 135 5e-06 Leucine rich repeat
LRR_14 PF23598 152 - 271 2e-09 Leucine-rich repeat region
LRR_14 PF23598 240 - 446 4.3e-14 Leucine-rich repeat region
LRR_14 PF23598 413 - 571 1.8e-06 Leucine-rich repeat region
LRR_8 PF13855 484 - 544 7.6e-07 Leucine rich repeat
LRR_8 PF13855 533 - 592 5.7e-09 Leucine rich repeat
Pkinase PF00069 711 - 980 5.6e-46 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 711 - 984 1.2e-43 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013993)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08590
fragaria_vesca FvH4_6g01540 FvH4_6g20240
malus_domestica MD00G1149400.v1.1 MD04G1232500.v1.1
prunus_persica Prupe.6G352100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0449301
rosa_laevigata RLG00000025841
rosa_multiflora Rmu_sc0007719.1_g000004 Rmu_sc0018234.1_g000001
rosa_roxburghii Rroxscaffold_6G00427300
rosa_rugosa Rorug02G0619500
rosa_samantha Rh3AG017500 Rh3BG017600 Rh3CG016600 Rh3DG017900
rosa_wichuraiana Rw3G001340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1779
Acc65I GGTACC 2 cut(s) 994, 2319
AccB1I GGYRCC 2 cut(s) 994, 2319
AccB7I CCANNNNNTGG 2 cut(s) 134, 863
AccI GTMKAC 2 cut(s) 1376, 2739
AccII CGCG 1 cut(s) 713
AciI CCGC 4 cut(s) 267, 711, 713, 2192
AclWI GGATC 4 cut(s) 119, 507, 520, 900
AcoI YGGCCR 2 cut(s) 1348, 2189
AcsI RAATTY 6 cut(s) 949, 1048, 1087, 1120, 1353, 2774
AcuI CTGAAG 7 cut(s) 161, 426, 520, 614, 722, 2199, 2286
AcyI GRCGYC 1 cut(s) 2553
AdeI CACNNNGTG 1 cut(s) 1871
AfeI AGCGCT 1 cut(s) 2820
AfiI CCNNNNNNNGG 8 cut(s) 134, 791, 863, 1008, 1796, 2651, 2750, 3044
AhlI ACTAGT 1 cut(s) 299
AjnI CCWGG 6 cut(s) 528, 888, 1144, 1293, 2227, 2856
Alw21I GWGCWC 2 cut(s) 67, 1876
Alw26I GTCTC 6 cut(s) 662, 770, 1405, 1850, 2597, 2928
AlwI GGATC 4 cut(s) 119, 507, 520, 900
AlwNI CAGNNNCTG 3 cut(s) 241, 592, 961
Aor51HI AGCGCT 1 cut(s) 2820
ApeKI GCWGC 4 cut(s) 652, 955, 1886, 2025
ApoI RAATTY 6 cut(s) 949, 1048, 1087, 1120, 1353, 2774
ArsI GACNNNNNNTTYG 4 cut(s) 389, 421, 2732, 2764
Asp700I GAANNNNTTC 3 cut(s) 439, 2778, 2868
Asp718I GGTACC 2 cut(s) 994, 2319
AspLEI GCGC 1 cut(s) 2821
AspS9I GGNCC 5 cut(s) 1061, 1243, 1781, 2254, 2995
AsuII TTCGAA 1 cut(s) 2782
AvaII GGWCC 5 cut(s) 1061, 1243, 1781, 2254, 2995
AxyI CCTNAGG 1 cut(s) 1359
BalI TGGCCA 1 cut(s) 1350
BanI GGYRCC 2 cut(s) 994, 2319
BanII GRGCYC 1 cut(s) 67
BbsI GAAGAC 3 cut(s) 1107, 1304, 1464
Bbv12I GWGCWC 2 cut(s) 67, 1876
BbvCI CCTCAGC 1 cut(s) 57
BbvI GCAGC 4 cut(s) 664, 967, 1873, 2037
BccI CCATC 4 cut(s) 128, 1989, 2193, 2932
BciT130I CCWGG 6 cut(s) 530, 890, 1146, 1295, 2229, 2858
BciVI GTATCC 4 cut(s) 677, 2362, 2627, 2648
BcoDI GTCTC 6 cut(s) 662, 770, 1405, 1850, 2597, 2928
BcuI ACTAGT 1 cut(s) 299
BfmI CTRYAG 1 cut(s) 2689
BfoI RGCGCY 1 cut(s) 2822
BfuI GTATCC 4 cut(s) 677, 2362, 2627, 2648
BglI GCCNNNNNGGC 1 cut(s) 2071
BisI GCNGC 6 cut(s) 268, 653, 956, 1887, 2026, 2192
BlpI GCTNAGC 1 cut(s) 930
BlsI GCNGC 6 cut(s) 269, 654, 957, 1888, 2027, 2193
Bme1390I CCNGG 6 cut(s) 530, 890, 1146, 1295, 2229, 2858
Bme18I GGWCC 5 cut(s) 1061, 1243, 1781, 2254, 2995
BmgT120I GGNCC 5 cut(s) 1061, 1243, 1781, 2254, 2995
BmiI GGNNCC 6 cut(s) 996, 1409, 1782, 2288, 2321, 2996
BmrFI CCNGG 6 cut(s) 530, 890, 1146, 1295, 2229, 2858
BmrI ACTGGG 2 cut(s) 1280, 1998
BmsI GCATC 8 cut(s) 390, 473, 1245, 1740, 1912, 2580, 2781, 2841
BmuI ACTGGG 2 cut(s) 1280, 1998
BpiI GAAGAC 3 cut(s) 1107, 1304, 1464
BpmI CTGGAG 3 cut(s) 205, 225, 2812
Bpu10I CCTNAGC 1 cut(s) 57
Bpu1102I GCTNAGC 1 cut(s) 930
Bpu14I TTCGAA 1 cut(s) 2782
BpuEI CTTGAG 3 cut(s) 542, 1055, 1796
BsaBI GATNNNNATC 1 cut(s) 2544
BsaHI GRCGYC 1 cut(s) 2553
BsaI GGTCTC 1 cut(s) 1850
BsaJI CCNNGG 8 cut(s) 711, 1078, 1294, 1743, 2066, 2228, 2278, 2966
BsaWI WCCGGW 2 cut(s) 626, 2307
Bsc4I CCNNNNNNNGG 8 cut(s) 134, 791, 863, 1008, 1796, 2651, 2750, 3044
Bse118I RCCGGY 4 cut(s) 1789, 1841, 1899, 2734
Bse21I CCTNAGG 1 cut(s) 1359
Bse8I GATNNNNATC 1 cut(s) 2544
BseBI CCWGG 6 cut(s) 530, 890, 1146, 1295, 2229, 2858
BseDI CCNNGG 8 cut(s) 711, 1078, 1294, 1743, 2066, 2228, 2278, 2966
BseGI GGATG 4 cut(s) 139, 1260, 2589, 2770
BseJI GATNNNNATC 1 cut(s) 2544
BseLI CCNNNNNNNGG 8 cut(s) 134, 791, 863, 1008, 1796, 2651, 2750, 3044
BseMII CTCAG 8 cut(s) 48, 368, 781, 921, 1373, 1601, 2893, 2976
BseX3I CGGCCG 1 cut(s) 2189
BseXI GCAGC 4 cut(s) 664, 967, 1873, 2037
BseYI CCCAGC 1 cut(s) 2950
Bsh1236I CGCG 1 cut(s) 713
Bsh1285I CGRYCG 1 cut(s) 2192
BshNI GGYRCC 2 cut(s) 994, 2319
BsiEI CGRYCG 1 cut(s) 2192
BsiHKAI GWGCWC 2 cut(s) 67, 1876
BsiSI CCGG 9 cut(s) 627, 1131, 1790, 1842, 1900, 2290, 2308, 2735, 2838
BslFI GGGAC 3 cut(s) 340, 2245, 3008
BslI CCNNNNNNNGG 8 cut(s) 134, 791, 863, 1008, 1796, 2651, 2750, 3044
BsmAI GTCTC 6 cut(s) 662, 770, 1405, 1850, 2597, 2928
BsmFI GGGAC 3 cut(s) 340, 2245, 3008
BsmI GAATGC 3 cut(s) 1437, 2194, 2849
Bso31I GGTCTC 1 cut(s) 1850
Bsp119I TTCGAA 1 cut(s) 2782
Bsp1286I GDGCHC 2 cut(s) 67, 1876
Bsp1407I TGTACA 2 cut(s) 232, 825
Bsp143I GATC 8 cut(s) 124, 280, 499, 525, 892, 1684, 1825, 2398
Bsp1720I GCTNAGC 1 cut(s) 930
BspACI CCGC 4 cut(s) 267, 711, 713, 2192
BspCNI CTCAG 8 cut(s) 49, 369, 780, 922, 1372, 1602, 2892, 2977
BspFNI CGCG 1 cut(s) 713
BspLI GGNNCC 6 cut(s) 996, 1409, 1782, 2288, 2321, 2996
BspPI GGATC 4 cut(s) 119, 507, 520, 900
BspQI GCTCTTC 1 cut(s) 430
BspT104I TTCGAA 1 cut(s) 2782
BspT107I GGYRCC 2 cut(s) 994, 2319
BspTNI GGTCTC 1 cut(s) 1850
BsrFI RCCGGY 4 cut(s) 1789, 1841, 1899, 2734
BsrGI TGTACA 2 cut(s) 232, 825
BssAI RCCGGY 4 cut(s) 1789, 1841, 1899, 2734
BssECI CCNNGG 8 cut(s) 711, 1078, 1294, 1743, 2066, 2228, 2278, 2966
BssMI GATC 8 cut(s) 124, 280, 499, 525, 892, 1684, 1825, 2398
BssNI GRCGYC 1 cut(s) 2553
BssT1I CCWWGG 5 cut(s) 1078, 1743, 2066, 2278, 2966
Bst2UI CCWGG 6 cut(s) 530, 890, 1146, 1295, 2229, 2858
Bst4CI ACNGT 5 cut(s) 73, 817, 2036, 2489, 2608
Bst6I CTCTTC 1 cut(s) 430
BstACI GRCGYC 1 cut(s) 2553
BstAUI TGTACA 2 cut(s) 232, 825
BstBI TTCGAA 1 cut(s) 2782
BstDSI CCRYGG 1 cut(s) 711
BstF5I GGATG 4 cut(s) 139, 1260, 2589, 2770
BstFNI CGCG 1 cut(s) 713
BstH2I RGCGCY 1 cut(s) 2822
BstHHI GCGC 1 cut(s) 2821
BstKTI GATC 8 cut(s) 127, 283, 502, 528, 895, 1687, 1828, 2401
BstMAI GTCTC 6 cut(s) 662, 770, 1405, 1850, 2597, 2928
BstMBI GATC 8 cut(s) 124, 280, 499, 525, 892, 1684, 1825, 2398
BstMCI CGRYCG 1 cut(s) 2192
BstMWI GCNNNNNNNGC 9 cut(s) 62, 935, 1495, 1534, 1731, 1892, 2071, 2224, 2561
BstNI CCWGG 6 cut(s) 530, 890, 1146, 1295, 2229, 2858
BstNSI RCATGY 3 cut(s) 1915, 2365, 2855
BstSCI CCNGG 6 cut(s) 528, 888, 1144, 1293, 2227, 2856
BstSFI CTRYAG 1 cut(s) 2689
BstUI CGCG 1 cut(s) 713
BstV1I GCAGC 4 cut(s) 664, 967, 1873, 2037
BstV2I GAAGAC 3 cut(s) 1107, 1304, 1464
BstX2I RGATCY 3 cut(s) 499, 525, 892
BstXI CCANNNNNNTGG 3 cut(s) 1101, 1562, 1634
BstYI RGATCY 3 cut(s) 499, 525, 892
BstZI CGGCCG 1 cut(s) 2189
Bsu36I CCTNAGG 1 cut(s) 1359
BsuI GTATCC 4 cut(s) 677, 2362, 2627, 2648
BtgI CCRYGG 1 cut(s) 711
BtgZI GCGATG 1 cut(s) 2818
BtsCI GGATG 4 cut(s) 139, 1260, 2589, 2770
BtsI GCAGTG 1 cut(s) 344
BtsIMutI CAGTG 8 cut(s) 344, 374, 775, 1070, 1539, 1639, 1791, 1986
CaiI CAGNNNCTG 3 cut(s) 241, 592, 961
CfoI GCGC 1 cut(s) 2821
Cfr10I RCCGGY 4 cut(s) 1789, 1841, 1899, 2734
Cfr13I GGNCC 5 cut(s) 1061, 1243, 1781, 2254, 2995
Cfr42I CCGCGG 1 cut(s) 714
CseI GACGC 1 cut(s) 2561
CsiI ACCWGGT 1 cut(s) 1144
CspCI CAANNNNNGTGG 6 cut(s) 502, 537, 771, 806, 1131, 1166
CviAII CATG 9 cut(s) 256, 1468, 1912, 2149, 2362, 2393, 2483, 2662, 2852
DpnI GATC 8 cut(s) 126, 282, 501, 527, 894, 1686, 1827, 2400
DpnII GATC 8 cut(s) 124, 280, 499, 525, 892, 1684, 1825, 2398
DraIII CACNNNGTG 1 cut(s) 1871
DrdI GACNNNNNNGTC 1 cut(s) 1779
DseDI GACNNNNNNGTC 1 cut(s) 1779
EaeI YGGCCR 2 cut(s) 1348, 2189
EagI CGGCCG 1 cut(s) 2189
Eam1104I CTCTTC 1 cut(s) 430
EarI CTCTTC 1 cut(s) 430
Ecl136II GAGCTC 1 cut(s) 65
EclXI CGGCCG 1 cut(s) 2189
Eco130I CCWWGG 5 cut(s) 1078, 1743, 2066, 2278, 2966
Eco147I AGGCCT 2 cut(s) 606, 1221
Eco24I GRGCYC 1 cut(s) 67
Eco31I GGTCTC 1 cut(s) 1850
Eco32I GATATC 3 cut(s) 1138, 2515, 2686
Eco47I GGWCC 5 cut(s) 1061, 1243, 1781, 2254, 2995
Eco47III AGCGCT 1 cut(s) 2820
Eco52I CGGCCG 1 cut(s) 2189
Eco53kI GAGCTC 1 cut(s) 65
Eco57I CTGAAG 7 cut(s) 161, 426, 520, 614, 722, 2199, 2286
Eco81I CCTNAGG 1 cut(s) 1359
EcoICRI GAGCTC 1 cut(s) 65
EcoO109I RGGNCCY 1 cut(s) 2254
EcoRI GAATTC 2 cut(s) 1048, 1087
EcoRII CCWGG 6 cut(s) 528, 888, 1144, 1293, 2227, 2856
EcoRV GATATC 3 cut(s) 1138, 2515, 2686
EcoT14I CCWWGG 5 cut(s) 1078, 1743, 2066, 2278, 2966
EcoT38I GRGCYC 1 cut(s) 67
ErhI CCWWGG 5 cut(s) 1078, 1743, 2066, 2278, 2966
FaeI CATG 9 cut(s) 259, 1471, 1915, 2152, 2365, 2396, 2486, 2665, 2855
FalI AAGNNNNNCTT 6 cut(s) 2000, 2032, 2853, 2885, 3008, 3040
FaqI GGGAC 3 cut(s) 340, 2245, 3008
FatI CATG 9 cut(s) 255, 1467, 1911, 2148, 2361, 2392, 2482, 2661, 2851
FauI CCCGC 1 cut(s) 718
FblI GTMKAC 2 cut(s) 1376, 2739
Fnu4HI GCNGC 6 cut(s) 268, 653, 956, 1887, 2026, 2192
FokI GGATG 4 cut(s) 146, 1267, 2596, 2777
FriOI GRGCYC 1 cut(s) 67
Fsp4HI GCNGC 6 cut(s) 268, 653, 956, 1887, 2026, 2192
GlaI GCGC 1 cut(s) 2820
GluI GCNGC 6 cut(s) 268, 653, 956, 1887, 2026, 2192
GsaI CCCAGC 1 cut(s) 2954
GsuI CTGGAG 3 cut(s) 205, 225, 2812
HaeII RGCGCY 1 cut(s) 2822
HapII CCGG 9 cut(s) 627, 1131, 1790, 1842, 1900, 2290, 2308, 2735, 2838
HgaI GACGC 1 cut(s) 2561
HhaI GCGC 1 cut(s) 2821
Hin1I GRCGYC 1 cut(s) 2553
Hin1II CATG 9 cut(s) 259, 1471, 1915, 2152, 2365, 2396, 2486, 2665, 2855
Hin6I GCGC 1 cut(s) 2819
HinP1I GCGC 1 cut(s) 2819
HincII GTYRAC 3 cut(s) 6, 460, 945
HindII GTYRAC 3 cut(s) 6, 460, 945
HindIII AAGCTT 2 cut(s) 1004, 1723
HpaII CCGG 9 cut(s) 627, 1131, 1790, 1842, 1900, 2290, 2308, 2735, 2838
Hpy166II GTNNAC 8 cut(s) 6, 185, 460, 886, 945, 1377, 2170, 2740
Hpy8I GTNNAC 8 cut(s) 6, 185, 460, 886, 945, 1377, 2170, 2740
Hpy99I CGWCG 1 cut(s) 1528
HpyAV CCTTC 5 cut(s) 136, 548, 1207, 1474, 2659
HpyCH4III ACNGT 5 cut(s) 73, 817, 2036, 2489, 2608
HpyF10VI GCNNNNNNNGC 9 cut(s) 62, 935, 1495, 1534, 1731, 1892, 2071, 2224, 2561
Hsp92I GRCGYC 1 cut(s) 2553
Hsp92II CATG 9 cut(s) 259, 1471, 1915, 2152, 2365, 2396, 2486, 2665, 2855
HspAI GCGC 1 cut(s) 2819
KpnI GGTACC 2 cut(s) 998, 2323
KroI GCCGGC 2 cut(s) 1789, 1899
KroNI GCCGGC 2 cut(s) 1791, 1901
KspI CCGCGG 1 cut(s) 714
Kzo9I GATC 8 cut(s) 124, 280, 499, 525, 892, 1684, 1825, 2398
LguI GCTCTTC 1 cut(s) 430
LmnI GCTCC 4 cut(s) 62, 543, 1503, 2292
Lsp1109I GCAGC 4 cut(s) 664, 967, 1873, 2037
LweI GCATC 8 cut(s) 390, 473, 1245, 1740, 1912, 2580, 2781, 2841
MabI ACCWGGT 1 cut(s) 1144
MaeIII GTNAC 6 cut(s) 395, 614, 866, 1622, 1975, 2108
MalI GATC 8 cut(s) 126, 282, 501, 527, 894, 1686, 1827, 2400
MboI GATC 8 cut(s) 124, 280, 499, 525, 892, 1684, 1825, 2398
MflI RGATCY 3 cut(s) 499, 525, 892
MhlI GDGCHC 2 cut(s) 67, 1876
MlsI TGGCCA 1 cut(s) 1350
MluNI TGGCCA 1 cut(s) 1350
MlyI GAGTC 4 cut(s) 1615, 1965, 2819, 2998
MmeI TCCRAC 6 cut(s) 276, 387, 590, 739, 1500, 1716
Mox20I TGGCCA 1 cut(s) 1350
MroNI GCCGGC 2 cut(s) 1789, 1899
MroXI GAANNNNTTC 3 cut(s) 439, 2778, 2868
MscI TGGCCA 1 cut(s) 1350
MseI TTAA 6 cut(s) 296, 968, 1016, 1755, 1805, 2211
MslI CAYNNNNRTG 4 cut(s) 389, 1099, 2147, 2598
Msp20I TGGCCA 1 cut(s) 1350
MspA1I CMGCKG 3 cut(s) 267, 713, 929
MspI CCGG 9 cut(s) 627, 1131, 1790, 1842, 1900, 2290, 2308, 2735, 2838
MspR9I CCNGG 6 cut(s) 530, 890, 1146, 1295, 2229, 2858
Mva1269I GAATGC 3 cut(s) 1437, 2194, 2849
MvaI CCWGG 6 cut(s) 530, 890, 1146, 1295, 2229, 2858
MvnI CGCG 1 cut(s) 713
MwoI GCNNNNNNNGC 9 cut(s) 62, 935, 1495, 1534, 1731, 1892, 2071, 2224, 2561
NaeI GCCGGC 2 cut(s) 1791, 1901
NdeII GATC 8 cut(s) 124, 280, 499, 525, 892, 1684, 1825, 2398
NgoMIV GCCGGC 2 cut(s) 1789, 1899
NlaIII CATG 9 cut(s) 259, 1471, 1915, 2152, 2365, 2396, 2486, 2665, 2855
NlaIV GGNNCC 6 cut(s) 996, 1409, 1782, 2288, 2321, 2996
NmeAIII GCCGAG 1 cut(s) 183
NmuCI GTSAC 3 cut(s) 395, 1975, 2108
NspI RCATGY 3 cut(s) 1915, 2365, 2855
NspV TTCGAA 1 cut(s) 2782
PaeI GCATGC 1 cut(s) 1915
PceI AGGCCT 2 cut(s) 606, 1221
PciSI GCTCTTC 1 cut(s) 430
PctI GAATGC 3 cut(s) 1437, 2194, 2849
PdiI GCCGGC 2 cut(s) 1791, 1901
PdmI GAANNNNTTC 3 cut(s) 439, 2778, 2868
PflMI CCANNNNNTGG 2 cut(s) 134, 863
PfoI TCCNGGA 2 cut(s) 528, 2856
PkrI GCNGC 6 cut(s) 269, 654, 957, 1888, 2027, 2193
PleI GAGTC 4 cut(s) 1614, 1964, 2819, 2998
PpsI GAGTC 4 cut(s) 1614, 1964, 2819, 2998
PpuMI RGGWCCY 1 cut(s) 2254
Psp124BI GAGCTC 1 cut(s) 67
Psp5II RGGWCCY 1 cut(s) 2254
Psp6I CCWGG 6 cut(s) 528, 888, 1144, 1293, 2227, 2856
PspFI CCCAGC 1 cut(s) 2950
PspGI CCWGG 6 cut(s) 528, 888, 1144, 1293, 2227, 2856
PspN4I GGNNCC 6 cut(s) 996, 1409, 1782, 2288, 2321, 2996
PspPI GGNCC 5 cut(s) 1061, 1243, 1781, 2254, 2995
PspPPI RGGWCCY 1 cut(s) 2254
PstNI CAGNNNCTG 3 cut(s) 241, 592, 961
PsuI RGATCY 3 cut(s) 499, 525, 892
PvuII CAGCTG 1 cut(s) 929
RseI CAYNNNNRTG 4 cut(s) 389, 1099, 2147, 2598
SacI GAGCTC 1 cut(s) 67
SacII CCGCGG 1 cut(s) 714
SapI GCTCTTC 1 cut(s) 430
SaqAI TTAA 6 cut(s) 296, 968, 1016, 1755, 1805, 2211
SatI GCNGC 6 cut(s) 268, 653, 956, 1887, 2026, 2192
Sau3AI GATC 8 cut(s) 124, 280, 499, 525, 892, 1684, 1825, 2398
Sau96I GGNCC 5 cut(s) 1061, 1243, 1781, 2254, 2995
SchI GAGTC 4 cut(s) 1615, 1965, 2819, 2998
ScrFI CCNGG 6 cut(s) 530, 890, 1146, 1295, 2229, 2858
SduI GDGCHC 2 cut(s) 67, 1876
SexAI ACCWGGT 1 cut(s) 1144
SfaNI GCATC 8 cut(s) 390, 473, 1245, 1740, 1912, 2580, 2781, 2841
SfcI CTRYAG 1 cut(s) 2689
Sfr303I CCGCGG 1 cut(s) 714
SfuI TTCGAA 1 cut(s) 2782
SgrBI CCGCGG 1 cut(s) 714
SinI GGWCC 5 cut(s) 1061, 1243, 1781, 2254, 2995
SmiMI CAYNNNNRTG 4 cut(s) 389, 1099, 2147, 2598
SmlI CTYRAG 3 cut(s) 521, 1034, 1775
SmoI CTYRAG 3 cut(s) 521, 1034, 1775
SpeI ACTAGT 1 cut(s) 299
SphI GCATGC 1 cut(s) 1915
SseBI AGGCCT 2 cut(s) 606, 1221
SsiI CCGC 4 cut(s) 267, 711, 713, 2192
SstI GAGCTC 1 cut(s) 67
StuI AGGCCT 2 cut(s) 606, 1221
StyD4I CCNGG 6 cut(s) 528, 888, 1144, 1293, 2227, 2856
StyI CCWWGG 5 cut(s) 1078, 1743, 2066, 2278, 2966
TaaI ACNGT 5 cut(s) 73, 817, 2036, 2489, 2608
TaqI TCGA 3 cut(s) 467, 2538, 2782
TatI WGTACW 4 cut(s) 232, 825, 2358, 2897
TauI GCSGC 2 cut(s) 270, 2194
Tru1I TTAA 6 cut(s) 296, 968, 1016, 1755, 1805, 2211
Tru9I TTAA 6 cut(s) 296, 968, 1016, 1755, 1805, 2211
TscAI CASTG 8 cut(s) 351, 381, 775, 1077, 1539, 1639, 1791, 1993
TseFI GTSAC 3 cut(s) 395, 1975, 2108
TseI GCWGC 4 cut(s) 652, 955, 1886, 2025
Tsp45I GTSAC 3 cut(s) 395, 1975, 2108
TspDTI ATGAA 7 cut(s) 99, 272, 1381, 1456, 2346, 2678, 2688
TspGWI ACGGA 2 cut(s) 1235, 2667
TspRI CASTG 8 cut(s) 351, 381, 775, 1077, 1539, 1639, 1791, 1993
Van91I CCANNNNNTGG 2 cut(s) 134, 863
VpaK11BI GGWCC 5 cut(s) 1061, 1243, 1781, 2254, 2995
XapI RAATTY 6 cut(s) 949, 1048, 1087, 1120, 1353, 2774
XceI RCATGY 3 cut(s) 1915, 2365, 2855
XmiI GTMKAC 2 cut(s) 1376, 2739
XmnI GAANNNNTTC 3 cut(s) 439, 2778, 2868
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.