MD04G1232500.v1.1
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0013993
Physical Location & Seq
Reverse (-)
31164329 .. 31167863
3535 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1232500.v1.1.491

Sequence Viewer

Length: 3045 bp
ATGTCAACCCTCTTGGTGTTCTTCTACTGTTTCGCTGCCACCACTCTTGTTTTTGCTCAAACCGGGCAGAATGTGGAGTGGTCGACCTTACTTTCGATCAAAGCTAGTCTCGTTGATCCAACCGGCGGCCTCAATGATTGGAAAATGCCGAGCAACGCAGTTGAGGGAGGTTCGCCACATTGCAACTGGACCGGAGTTTGGTGCAACTCAAAAGGGTTCGTAGAGAAGCTGGACATCTCCAACATGAACCTCAGCGGCCATGTTTCCGATCACATTCAAGGCTTGCGCCGTCTTTCGATCCTCAACATATCTTGCAACGCGTTTGAAACATCGTTGCCAAGGCTGATAACCAATCTTACGTCGCTGAAGAGCATTGACGTGAGCCAAAACAACTTTGTTGGCGAGTTTCCAACGGGTTTCGGAAGGGCTTCAGGACTCACATCAGTCAATGCATCAAGCAACAACTTCTCAGGATTTCTTCCGGATGATCTTGGATACGCAACATCCCTCGAAATCCTAGATTTCAGAGGGAGCTTCTTTGAAGGCTCGATCCCTACATCTTACAAGAACCTGCAGAAGCTGAAGTTTCTTGGCCTATCTGGCAACAATCTCACCGGAAACCTTCCAAGAGAATTCGGGCAGCTTTCATCACTAGAGACTATTATTCTCGGATACAACGCTTTTGAAGGCGAAATCCCAGCGGAGTTTGGCAACCTCACCAATCTTCAGTATCTTGACTTGGCAGTTGGCAGTCTCAGCGGCCAAATTCCACCTGAATTGAGTAGACTGCAAAAACTCACCACAGTTTATTTGTACAAAAACAATTTCACAGGAAAAATCCCACCAGATTTCGGTAACATTACATCGCTAGCGTTCTTGGATCTCTCCGATAATCAGATTTCCGGAGAGATTCCTGCAGAGCTTGCACAATTGACGAATCTGCAGCTTCTGAACTTAATGTGCAATAAGCTCACAGGTTCAGTTCCTGCCAACCTCGGCAAGTTAACCAAGTTACAGGTACTTGAGCTGTGGAAGAACTCGTTGACAGGTCCTCTGCCAAGAAACCTTGGAAGGAATTCCCCGTTGCAATGGTTAGACGTGTCATCGAACTCATTGTCGGGTGATCTCCCACCAGGTTTGTGTGATTCCGGCAATCTCACTAAACTCATCCTGTTCAACAACTCCTTCTCCGGTCCAATCCCAGTAAGGCTTTCGACATGTTTGTCATTGGTTCGTGTTCGTATGCAAAACAATCTTATTTCGGGATCCATTCCAGTTGGTCTGGGAAATCTTCCAATTCTTCAGAGGCTAGAATTGGCGAAAAACAATCTTACTGGCCAAATTCCTGCTGACATTACTCTGTCTGCGTCTCTTTCCTTCATCGATGTCTCTTGGAACCGCCTCGAATCATATCTTCCTTCGAGTATCCTTTCGCTTCCTAATCTTCAAACCTTCATGGCTTCCAATAACAACTTGCAAGGCAAGCTGCCGGACCAGTTCCAAGACTGTCCGTCACTCTCAGTCCTCGACATTTCAAACAACCACATCTCCGGAAAAATTCCAGAGAGCATAGCTTCTTGCGAGAAGCTGGTGAACCTGAACCTCAGAAACAACCAGTTCAACGGGGAAATCCCTCAATCAATAGCGACAATGCATACATTGTCAGTTCTCGACCTGTCCAACAATTCTTTGGTAGGTCAAATCCCGGAAAGTTTCGGAAGCTCACCAGCCTTGGAGATGCTTAACTTGTCTTACAACAGGCTTGAAGGTCCAGTACCTGCAAATGGTATCCTAATGACCCTAAACCCTAACGATCTCCTAGGCAATGCCGGTCTTTGCGGAGGCATACTTCCACCGTGCTCTCACAGTTTAGCCGCAACAGCAGCTCCTATTCGGAATATGCACATCAAACACATCCTTACTGGATTCATCGTTGGAATCTCAGTAATTTTGACTGTGGGAACCGCATTTCTCGCAGGGAGATGGATGTATCGGAAATGGTACTTATATAACAGCTTCGACAGTTGGTTTAACAAGACCAATCAAGAGTGGCCTTGGAGGTTAGTAGCATTTCAGAGAATTAACTTCACAAGTGCTGACATTCTGGCTTGCATACAAGAATCGAATATCATTGGCATGGGCGGGAGCGGAGTTGTTTACAAGGCGGAAATCCATCGACCCCACTCGGTTGTAGCAGTTAAGAAGCTATGGAGACCGGGGACAGACGTTGAAAGTGGTGATGATCTGTTTGGTGAAGTGAACCTCCTCGGCAGGCTCCGGCATAGAAATATTGTTCGGTTGCTAGGGTACCTTCATAACGAAAGCGACGTTGTGATGATATACGAGTTCATGCCGAACGGGAACCTCGGGACTGCATTGCATGGAAATCAAGCCGGAAAGTTGCTGGTCGACTGGGTTTCGAGGTATAACATAGCAGTGGGTGTTGCACAAGGTCTGAACTATCTCCACCACGACTGTCAGCCGCCAGTTATTCACCGAGATATCAAATCGAACAACATTCTGCTGGATGCAAACCTTGACGCCAGAGTGGCGGATTTCGGGCTAGCAAGGATGATGATCCACAAGAACGAGACAGTTTCAATGGTGGCGGGATCTTACGGATACATTGCACCTGAATACGGATACGCATTGAAGGTAGACGAAAAGACTGACATCTACAGCTACGGCGTAGTTCTGTTAGAGCTTCTAACCGGAAAGATGCCGGTAGACCCTGCGTTCGGGGAATCTGTGGATATCGTGGAGTGGGTTCGAAGGAAGATGAGGACCAAGAGAGCCTTAGAAGAAACACTGGATCCTAGCATAGCCGGTGAATGCATGCATGTTCAAGAAGAAATGCTTCTCGTCCTCCGAATCGCGCTTGTCTGTACGGCAAAGCTCCCGAAAGACAGGCCTTCCATGAGAGACATAATAACAATGCTCGGAGAGGCGAAACCGAGGAGGAAAAGCATTTGTGAGAGTAGAAGCCAGAAAATTGGCACGGAGAAGCCTATATTCAGCACCTCACCAGTCATGGGTCTTCTGTAG

Protein Analysis

1015

Amino Acids

111.15

Weight (kDa)

6.44

Isoelectric Point (pI)

39.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 28 - 69 3.3e-06 Leucine rich repeat N-terminal domain
LRR_14 PF23598 189 - 267 1.1e-07 Leucine-rich repeat region
LRR_8 PF13855 193 - 247 8.8e-06 Leucine rich repeat
LRR_14 PF23598 235 - 441 3.6e-14 Leucine-rich repeat region
LRR_8 PF13855 480 - 540 3.9e-09 Leucine rich repeat
LRR_8 PF13855 529 - 588 8.2e-09 Leucine rich repeat
Pkinase PF00069 707 - 977 9.3e-45 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 708 - 979 5.1e-41 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013993)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G08590
fragaria_vesca FvH4_6g01540 FvH4_6g20240
malus_domestica MD00G1149400.v1.1 MD04G1232500.v1.1
prunus_persica Prupe.6G352100_v2.0.a1
rosa_chinensis RchiOBHm_Chr3g0449301
rosa_laevigata RLG00000025841
rosa_multiflora Rmu_sc0007719.1_g000004 Rmu_sc0018234.1_g000001
rosa_roxburghii Rroxscaffold_6G00427300
rosa_rugosa Rorug02G0619500
rosa_samantha Rh3AG017500 Rh3BG017600 Rh3CG016600 Rh3DG017900
rosa_wichuraiana Rw3G001340

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1222
Acc36I ACCTGC 2 cut(s) 579, 1786
Acc65I GGTACC 1 cut(s) 2307
AccB1I GGYRCC 1 cut(s) 2307
AccBSI CCGCTC 1 cut(s) 2148
AccI GTMKAC 5 cut(s) 83, 784, 2409, 2658, 2727
AccII CGCG 2 cut(s) 320, 2876
AccIII TCCGGA 3 cut(s) 481, 902, 1550
AcoI YGGCCR 3 cut(s) 256, 760, 1336
AcsI RAATTY 5 cut(s) 632, 765, 1075, 1341, 1557
AcuI CTGAAG 5 cut(s) 386, 414, 602, 710, 1286
AcyI GRCGYC 1 cut(s) 2541
AfaI GTAC 6 cut(s) 815, 1020, 1776, 2003, 2309, 2887
AfiI CCNNNNNNNGG 7 cut(s) 125, 198, 851, 1784, 2639, 2738, 3032
AflIII ACRYGT 3 cut(s) 318, 1098, 1217
AhdI GACNNNNNGTC 1 cut(s) 1510
AjiI CACGTC 2 cut(s) 379, 1099
AjnI CCWGG 1 cut(s) 1132
Alw21I GWGCWC 1 cut(s) 1862
Alw26I GTCTC 8 cut(s) 113, 650, 758, 1374, 1393, 2206, 2585, 2916
AlwNI CAGNNNCTG 4 cut(s) 580, 949, 986, 1778
Ama87I CYCGRG 1 cut(s) 2366
Aor13HI TCCGGA 3 cut(s) 481, 902, 1550
AoxI GGCC 7 cut(s) 127, 256, 592, 760, 1336, 2051, 2909
ApeKI GCWGC 5 cut(s) 35, 640, 943, 1486, 1883
ApoI RAATTY 5 cut(s) 632, 765, 1075, 1341, 1557
ArsI GACNNNNNNTTYG 6 cut(s) 76, 108, 1100, 1132, 2720, 2752
Asp700I GAANNNNTTC 3 cut(s) 427, 1075, 2856
Asp718I GGTACC 1 cut(s) 2307
AspA2I CCTAGG 1 cut(s) 1819
AspLEI GCGC 2 cut(s) 288, 2878
AspS9I GGNCC 6 cut(s) 189, 1049, 1193, 1492, 1769, 2784
AsuC2I CCSGG 3 cut(s) 64, 1706, 2217
AsuII TTCGAA 1 cut(s) 2770
AsuNHI GCTAGC 2 cut(s) 868, 2563
AvaI CYCGRG 1 cut(s) 2366
AvaII GGWCC 6 cut(s) 189, 1049, 1193, 1492, 1769, 2784
AvrII CCTAGG 1 cut(s) 1819
BalI TGGCCA 1 cut(s) 1338
BamHI GGATCC 2 cut(s) 1265, 2812
BanI GGYRCC 1 cut(s) 2307
BarI GAAGNNNNNNTAC 2 cut(s) 1758, 1790
BbsI GAAGAC 1 cut(s) 3029
Bbv12I GWGCWC 1 cut(s) 1862
BbvCI CCTCAGC 1 cut(s) 251
BbvI GCAGC 5 cut(s) 22, 652, 955, 1473, 1895
BccI CCATC 2 cut(s) 1977, 2181
BceAI ACGGC 3 cut(s) 273, 2701, 2904
BciT130I CCWGG 1 cut(s) 1134
BciVI GTATCC 6 cut(s) 488, 665, 1436, 1799, 2615, 2636
BcnI CCSGG 3 cut(s) 64, 1706, 2217
BcoDI GTCTC 8 cut(s) 113, 650, 758, 1374, 1393, 2206, 2585, 2916
BfaI CTAG 9 cut(s) 105, 518, 653, 869, 1310, 1820, 2303, 2564, 2817
BfmI CTRYAG 5 cut(s) 572, 915, 941, 2677, 3041
BfuAI ACCTGC 2 cut(s) 579, 1786
BfuI GTATCC 6 cut(s) 488, 665, 1436, 1799, 2615, 2636
BglI GCCNNNNNGGC 2 cut(s) 600, 2549
BlnI CCTAGG 1 cut(s) 1819
Bme1390I CCNGG 4 cut(s) 64, 1134, 1706, 2217
Bme18I GGWCC 6 cut(s) 189, 1049, 1193, 1492, 1769, 2784
BmeRI GACNNNNNGTC 1 cut(s) 1510
BmeT110I CYCGRG 1 cut(s) 2366
BmgBI CACGTC 2 cut(s) 379, 1099
BmgT120I GGNCC 6 cut(s) 189, 1049, 1193, 1492, 1769, 2784
BmiI GGNNCC 7 cut(s) 1267, 1397, 1963, 2276, 2309, 2363, 2814
BmrFI CCNGG 4 cut(s) 64, 1134, 1706, 2217
BmrI ACTGGG 2 cut(s) 1196, 2422
BmsI GCATC 4 cut(s) 461, 1728, 2518, 2709
BmtI GCTAGC 2 cut(s) 872, 2567
BmuI ACTGGG 2 cut(s) 1196, 2422
BpiI GAAGAC 1 cut(s) 3029
Bpu10I CCTNAGC 1 cut(s) 251
Bpu14I TTCGAA 1 cut(s) 2770
BpuEI CTTGAG 1 cut(s) 1043
BpuMI CCSGG 3 cut(s) 64, 1706, 2217
Bsa29I ATCGAT 1 cut(s) 1383
BsaBI GATNNNNATC 1 cut(s) 2576
BsaHI GRCGYC 1 cut(s) 2541
BsaI GGTCTC 1 cut(s) 2206
BsaWI WCCGGW 7 cut(s) 191, 481, 614, 902, 1190, 1550, 2711
BsaXI ACNNNNNCTCC 6 cut(s) 1172, 1202, 1532, 1562, 1870, 1900
Bsc4I CCNNNNNNNGG 7 cut(s) 125, 198, 851, 1784, 2639, 2738, 3032
Bse118I RCCGGY 4 cut(s) 122, 1829, 2722, 2825
Bse3DI GCAATG 5 cut(s) 178, 1094, 1831, 2375, 2625
Bse8I GATNNNNATC 1 cut(s) 2576
BseAI TCCGGA 3 cut(s) 481, 902, 1550
BseBI CCWGG 1 cut(s) 1134
BseCI ATCGAT 1 cut(s) 1383
BseGI GGATG 7 cut(s) 490, 503, 1167, 1914, 1992, 2533, 2577
BseJI GATNNNNATC 1 cut(s) 2576
BseLI CCNNNNNNNGG 7 cut(s) 125, 198, 851, 1784, 2639, 2738, 3032
BseMI GCAATG 5 cut(s) 178, 1094, 1831, 2375, 2625
BseMII CTCAG 6 cut(s) 265, 483, 769, 1533, 1618, 1956
BseRI GAGGAG 2 cut(s) 2255, 2971
BseXI GCAGC 5 cut(s) 22, 652, 955, 1473, 1895
BseYI CCCAGC 1 cut(s) 697
Bsh1236I CGCG 2 cut(s) 320, 2876
BshFI GGCC 7 cut(s) 129, 258, 594, 762, 1338, 2053, 2911
BshNI GGYRCC 1 cut(s) 2307
BshVI ATCGAT 1 cut(s) 1383
BsiHKAI GWGCWC 1 cut(s) 1862
BsiHKCI CYCGRG 1 cut(s) 2366
BslFI GGGAC 2 cut(s) 2233, 2383
BslI CCNNNNNNNGG 7 cut(s) 125, 198, 851, 1784, 2639, 2738, 3032
BsmAI GTCTC 8 cut(s) 113, 650, 758, 1374, 1393, 2206, 2585, 2916
BsmBI CGTCTC 1 cut(s) 1374
BsmFI GGGAC 2 cut(s) 2233, 2383
BsmI GAATGC 1 cut(s) 2837
BsnI GGCC 7 cut(s) 129, 258, 594, 762, 1338, 2053, 2911
Bso31I GGTCTC 1 cut(s) 2206
BsoBI CYCGRG 1 cut(s) 2366
Bsp119I TTCGAA 1 cut(s) 2770
Bsp1286I GDGCHC 1 cut(s) 1862
Bsp13I TCCGGA 3 cut(s) 481, 902, 1550
Bsp1407I TGTACA 1 cut(s) 813
BspANI GGCC 7 cut(s) 129, 258, 594, 762, 1338, 2053, 2911
BspCNI CTCAG 6 cut(s) 264, 482, 768, 1532, 1617, 1955
BspDI ATCGAT 1 cut(s) 1383
BspEI TCCGGA 3 cut(s) 481, 902, 1550
BspFNI CGCG 2 cut(s) 320, 2876
BspLI GGNNCC 7 cut(s) 1267, 1397, 1963, 2276, 2309, 2363, 2814
BspMAI CTGCAG 3 cut(s) 576, 919, 945
BspMI ACCTGC 2 cut(s) 579, 1786
BspOI GCTAGC 2 cut(s) 872, 2567
BspQI GCTCTTC 1 cut(s) 362
BspT104I TTCGAA 1 cut(s) 2770
BspT107I GGYRCC 1 cut(s) 2307
BspTNI GGTCTC 1 cut(s) 2206
BsrBI CCGCTC 1 cut(s) 2148
BsrDI GCAATG 5 cut(s) 178, 1094, 1831, 2375, 2625
BsrFI RCCGGY 4 cut(s) 122, 1829, 2722, 2825
BsrGI TGTACA 1 cut(s) 813
BssAI RCCGGY 4 cut(s) 122, 1829, 2722, 2825
BssNI GRCGYC 1 cut(s) 2541
BssT1I CCWWGG 5 cut(s) 338, 1066, 1731, 1819, 2054
Bst2UI CCWGG 1 cut(s) 1134
Bst4CI ACNGT 9 cut(s) 29, 805, 1508, 1857, 1868, 1957, 2024, 2477, 2596
Bst6I CTCTTC 1 cut(s) 362
BstACI GRCGYC 1 cut(s) 2541
BstAPI GCANNNNNTGC 1 cut(s) 923
BstAUI TGTACA 1 cut(s) 813
BstBI TTCGAA 1 cut(s) 2770
BstC8I GCNNGC 8 cut(s) 284, 870, 924, 1484, 2110, 2273, 2565, 2837
BstDEI CTNAG 7 cut(s) 251, 469, 755, 1519, 1604, 1942, 2797
BstF5I GGATG 7 cut(s) 490, 503, 1167, 1914, 1992, 2533, 2577
BstFNI CGCG 2 cut(s) 320, 2876
BstHHI GCGC 2 cut(s) 288, 2878
BstMAI GTCTC 8 cut(s) 113, 650, 758, 1374, 1393, 2206, 2585, 2916
BstMWI GCNNNNNNNGC 8 cut(s) 600, 756, 923, 1483, 1880, 1883, 1973, 2549
BstNI CCWGG 1 cut(s) 1134
BstNSI RCATGY 3 cut(s) 1221, 2839, 2843
BstSCI CCNGG 4 cut(s) 62, 1132, 1704, 2215
BstSFI CTRYAG 5 cut(s) 572, 915, 941, 2677, 3041
BstUI CGCG 2 cut(s) 320, 2876
BstV1I GCAGC 5 cut(s) 22, 652, 955, 1473, 1895
BstV2I GAAGAC 1 cut(s) 3029
BstX2I RGATCY 4 cut(s) 880, 1265, 2612, 2812
BstXI CCANNNNNNTGG 1 cut(s) 2993
BstYI RGATCY 4 cut(s) 880, 1265, 2612, 2812
Bsu15I ATCGAT 1 cut(s) 1383
BsuI GTATCC 6 cut(s) 488, 665, 1436, 1799, 2615, 2636
BsuRI GGCC 7 cut(s) 129, 258, 594, 762, 1338, 2053, 2911
BsuTUI ATCGAT 1 cut(s) 1383
BtgZI GCGATG 1 cut(s) 849
BtrI CACGTC 2 cut(s) 379, 1099
BtsCI GGATG 7 cut(s) 490, 503, 1167, 1914, 1992, 2533, 2577
BtsI GCAGTG 1 cut(s) 2442
BtsIMutI CAGTG 2 cut(s) 2442, 2807
BveI ACCTGC 2 cut(s) 579, 1786
Cac8I GCNNGC 8 cut(s) 284, 870, 924, 1484, 2110, 2273, 2565, 2837
CaiI CAGNNNCTG 4 cut(s) 580, 949, 986, 1778
CfoI GCGC 2 cut(s) 288, 2878
Cfr10I RCCGGY 4 cut(s) 122, 1829, 2722, 2825
Cfr13I GGNCC 6 cut(s) 189, 1049, 1193, 1492, 1769, 2784
ClaI ATCGAT 1 cut(s) 1383
CseI GACGC 2 cut(s) 1356, 2549
CsiI ACCWGGT 1 cut(s) 1132
Csp6I GTAC 6 cut(s) 814, 1019, 1775, 2002, 2308, 2886
CspCI CAANNNNNGTGG 6 cut(s) 28, 63, 759, 794, 1119, 1154
CviQI GTAC 6 cut(s) 814, 1019, 1775, 2002, 2308, 2886
DdeI CTNAG 7 cut(s) 251, 469, 755, 1519, 1604, 1942, 2797
DrdI GACNNNNNNGTC 1 cut(s) 1222
DriI GACNNNNNGTC 1 cut(s) 1510
DseDI GACNNNNNNGTC 1 cut(s) 1222
EaeI YGGCCR 3 cut(s) 256, 760, 1336
Eam1104I CTCTTC 1 cut(s) 362
Eam1105I GACNNNNNGTC 1 cut(s) 1510
EarI CTCTTC 1 cut(s) 362
EciI GGCGGA 2 cut(s) 2180, 2567
Eco130I CCWWGG 5 cut(s) 338, 1066, 1731, 1819, 2054
Eco147I AGGCCT 1 cut(s) 2911
Eco31I GGTCTC 1 cut(s) 2206
Eco32I GATATC 2 cut(s) 2503, 2755
Eco47I GGWCC 6 cut(s) 189, 1049, 1193, 1492, 1769, 2784
Eco57I CTGAAG 5 cut(s) 386, 414, 602, 710, 1286
Eco88I CYCGRG 1 cut(s) 2366
EcoO109I RGGNCCY 1 cut(s) 1049
EcoRI GAATTC 2 cut(s) 632, 1075
EcoRII CCWGG 1 cut(s) 1132
EcoRV GATATC 2 cut(s) 2503, 2755
EcoT14I CCWWGG 5 cut(s) 338, 1066, 1731, 1819, 2054
EcoT22I ATGCAT 4 cut(s) 454, 1656, 2837, 2841
ErhI CCWWGG 5 cut(s) 338, 1066, 1731, 1819, 2054
Esp3I CGTCTC 1 cut(s) 1374
FalI AAGNNNNNCTT 4 cut(s) 2780, 2812, 2841, 2873
FaqI GGGAC 2 cut(s) 2233, 2383
FauI CCCGC 2 cut(s) 2135, 2602
FblI GTMKAC 5 cut(s) 83, 784, 2409, 2658, 2727
FokI GGATG 7 cut(s) 490, 497, 1154, 1901, 1999, 2540, 2584
FspBI CTAG 9 cut(s) 105, 518, 653, 869, 1310, 1820, 2303, 2564, 2817
GlaI GCGC 2 cut(s) 287, 2877
GsaI CCCAGC 1 cut(s) 701
HaeIII GGCC 7 cut(s) 129, 258, 594, 762, 1338, 2053, 2911
HgaI GACGC 2 cut(s) 1356, 2549
HhaI GCGC 2 cut(s) 288, 2878
Hin1I GRCGYC 1 cut(s) 2541
Hin6I GCGC 2 cut(s) 286, 2876
HinP1I GCGC 2 cut(s) 286, 2876
HincII GTYRAC 5 cut(s) 6, 84, 1005, 1044, 2410
HindII GTYRAC 5 cut(s) 6, 84, 1005, 1044, 2410
HpaI GTTAAC 1 cut(s) 1005
Hpy99I CGWCG 2 cut(s) 364, 2330
HpyCH4III ACNGT 9 cut(s) 29, 805, 1508, 1857, 1868, 1957, 2024, 2477, 2596
HpyCH4IV ACGT 5 cut(s) 359, 378, 1098, 2226, 2328
HpyF10VI GCNNNNNNNGC 8 cut(s) 600, 756, 923, 1483, 1880, 1883, 1973, 2549
HpyF3I CTNAG 7 cut(s) 251, 469, 755, 1519, 1604, 1942, 2797
HpySE526I ACGT 5 cut(s) 359, 378, 1098, 2226, 2328
Hsp92I GRCGYC 1 cut(s) 2541
HspAI GCGC 2 cut(s) 286, 2876
Kpn2I TCCGGA 3 cut(s) 481, 902, 1550
KpnI GGTACC 1 cut(s) 2311
KspAI GTTAAC 1 cut(s) 1005
LguI GCTCTTC 1 cut(s) 362
LmnI GCTCC 5 cut(s) 531, 1891, 2145, 2280, 2901
Lsp1109I GCAGC 5 cut(s) 22, 652, 955, 1473, 1895
LweI GCATC 4 cut(s) 461, 1728, 2518, 2709
MabI ACCWGGT 1 cut(s) 1132
MaeI CTAG 9 cut(s) 105, 518, 653, 869, 1310, 1820, 2303, 2564, 2817
MaeII ACGT 5 cut(s) 359, 378, 1098, 2226, 2328
MaeIII GTNAC 3 cut(s) 854, 1011, 1512
MbiI CCGCTC 1 cut(s) 2148
MfeI CAATTG 1 cut(s) 929
MflI RGATCY 4 cut(s) 880, 1265, 2612, 2812
MhlI GDGCHC 1 cut(s) 1862
MlsI TGGCCA 1 cut(s) 1338
MluI ACGCGT 1 cut(s) 318
MluNI TGGCCA 1 cut(s) 1338
MlyI GAGTC 1 cut(s) 429
MmeI TCCRAC 5 cut(s) 143, 264, 434, 1704, 1915
Mox20I TGGCCA 1 cut(s) 1338
Mph1103I ATGCAT 4 cut(s) 454, 1656, 2837, 2841
MroI TCCGGA 3 cut(s) 481, 902, 1550
MroXI GAANNNNTTC 3 cut(s) 427, 1075, 2856
MscI TGGCCA 1 cut(s) 1338
MseI TTAA 6 cut(s) 956, 1004, 1743, 2031, 2082, 2199
MslI CAYNNNNRTG 3 cut(s) 377, 2135, 2435
Msp20I TGGCCA 1 cut(s) 1338
MspA1I CMGCKG 3 cut(s) 255, 701, 759
MspR9I CCNGG 4 cut(s) 64, 1134, 1706, 2217
MunI CAATTG 1 cut(s) 929
Mva1269I GAATGC 1 cut(s) 2837
MvaI CCWGG 1 cut(s) 1134
MvnI CGCG 2 cut(s) 320, 2876
MwoI GCNNNNNNNGC 8 cut(s) 600, 756, 923, 1483, 1880, 1883, 1973, 2549
NciI CCSGG 3 cut(s) 64, 1706, 2217
NheI GCTAGC 2 cut(s) 868, 2563
NlaIV GGNNCC 7 cut(s) 1267, 1397, 1963, 2276, 2309, 2363, 2814
NmeAIII GCCGAG 3 cut(s) 174, 975, 2247
NmuCI GTSAC 1 cut(s) 1512
NsiI ATGCAT 4 cut(s) 454, 1656, 2837, 2841
NspI RCATGY 3 cut(s) 1221, 2839, 2843
NspV TTCGAA 1 cut(s) 2770
PaeI GCATGC 1 cut(s) 2839
PceI AGGCCT 1 cut(s) 2911
PciI ACATGT 1 cut(s) 1217
PciSI GCTCTTC 1 cut(s) 362
PcsI WCGNNNNNNNCGW 1 cut(s) 2325
PctI GAATGC 1 cut(s) 2837
PdmI GAANNNNTTC 3 cut(s) 427, 1075, 2856
PfeI GAWTC 9 cut(s) 910, 937, 1145, 1406, 1926, 1938, 2120, 2744, 2871
PfoI TCCNGGA 1 cut(s) 1704
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
PpuMI RGGWCCY 1 cut(s) 1049
PscI ACATGT 1 cut(s) 1217
Psp5II RGGWCCY 1 cut(s) 1049
Psp6I CCWGG 1 cut(s) 1132
PspFI CCCAGC 1 cut(s) 697
PspGI CCWGG 1 cut(s) 1132
PspN4I GGNNCC 7 cut(s) 1267, 1397, 1963, 2276, 2309, 2363, 2814
PspPI GGNCC 6 cut(s) 189, 1049, 1193, 1492, 1769, 2784
PspPPI RGGWCCY 1 cut(s) 1049
PstI CTGCAG 3 cut(s) 576, 919, 945
PstNI CAGNNNCTG 4 cut(s) 580, 949, 986, 1778
PsuI RGATCY 4 cut(s) 880, 1265, 2612, 2812
RsaI GTAC 6 cut(s) 815, 1020, 1776, 2003, 2309, 2887
RsaNI GTAC 6 cut(s) 814, 1019, 1775, 2002, 2308, 2886
RseI CAYNNNNRTG 3 cut(s) 377, 2135, 2435
SalI GTCGAC 2 cut(s) 82, 2408
SapI GCTCTTC 1 cut(s) 362
SaqAI TTAA 6 cut(s) 956, 1004, 1743, 2031, 2082, 2199
Sau96I GGNCC 6 cut(s) 189, 1049, 1193, 1492, 1769, 2784
SchI GAGTC 1 cut(s) 429
ScrFI CCNGG 4 cut(s) 64, 1134, 1706, 2217
SduI GDGCHC 1 cut(s) 1862
SexAI ACCWGGT 1 cut(s) 1132
SfaNI GCATC 4 cut(s) 461, 1728, 2518, 2709
SfcI CTRYAG 5 cut(s) 572, 915, 941, 2677, 3041
SfuI TTCGAA 1 cut(s) 2770
SinI GGWCC 6 cut(s) 189, 1049, 1193, 1492, 1769, 2784
SmiMI CAYNNNNRTG 3 cut(s) 377, 2135, 2435
SmlI CTYRAG 1 cut(s) 1022
SmoI CTYRAG 1 cut(s) 1022
SphI GCATGC 1 cut(s) 2839
SseBI AGGCCT 1 cut(s) 2911
SspI AATATT 1 cut(s) 2290
SspMI CTAG 9 cut(s) 105, 518, 653, 869, 1310, 1820, 2303, 2564, 2817
StuI AGGCCT 1 cut(s) 2911
StyD4I CCNGG 4 cut(s) 62, 1132, 1704, 2215
StyI CCWWGG 5 cut(s) 338, 1066, 1731, 1819, 2054
TaaI ACNGT 9 cut(s) 29, 805, 1508, 1857, 1868, 1957, 2024, 2477, 2596
TaiI ACGT 5 cut(s) 362, 381, 1101, 2229, 2331
TatI WGTACW 1 cut(s) 813
TauI GCSGC 5 cut(s) 129, 258, 762, 1877, 2485
TfiI GAWTC 9 cut(s) 910, 937, 1145, 1406, 1926, 1938, 2120, 2744, 2871
Tru1I TTAA 6 cut(s) 956, 1004, 1743, 2031, 2082, 2199
Tru9I TTAA 6 cut(s) 956, 1004, 1743, 2031, 2082, 2199
TscAI CASTG 2 cut(s) 2442, 2814
TseFI GTSAC 1 cut(s) 1512
TseI GCWGC 5 cut(s) 35, 640, 943, 1486, 1883
Tsp45I GTSAC 1 cut(s) 1512
TspDTI ATGAA 7 cut(s) 260, 636, 1369, 1444, 1918, 2303, 2338
TspGWI ACGGA 4 cut(s) 1500, 2634, 2655, 3014
TspRI CASTG 2 cut(s) 2442, 2814
VpaK11BI GGWCC 6 cut(s) 189, 1049, 1193, 1492, 1769, 2784
XapI RAATTY 5 cut(s) 632, 765, 1075, 1341, 1557
XceI RCATGY 3 cut(s) 1221, 2839, 2843
XcmI CCANNNNNNNNNTGG 2 cut(s) 183, 1687
XmaJI CCTAGG 1 cut(s) 1819
XmiI GTMKAC 5 cut(s) 83, 784, 2409, 2658, 2727
XmnI GAANNNNTTC 3 cut(s) 427, 1075, 2856
XspI CTAG 9 cut(s) 105, 518, 653, 869, 1310, 1820, 2303, 2564, 2817
Zsp2I ATGCAT 4 cut(s) 454, 1656, 2837, 2841
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.