Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0013366

Physical Location & Seq

Genomic Coordinates Reverse (-)

44274525 .. 44278279

Gene Structure 3755 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1186200.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 858 bp

ATGGCTTCCAACATTGGGATTATGGACAGTGCTTACTTTGTTGGGAGGAATGAGATTTTGACGTGGATCAACAATCGCCTTCAGCTGAACCTCTCTCGCATTGAAGAGGCTGCATCTGGTGCTGTGCAATGTCAAATGATGGATATGACCTATCCTGGTGTTGTTCCAATGCACAAGGTCAATTTTGATGCGAAAACAGAATATGATATGATCCAGAATTATAAGGTTCTACAGGAAGTATTCAACAAGTTAAAAATTGCGAAGCATCTTGAAGTCAATAGGCTGGTTAAAGGCAGGCCTTTGGACAACTTGGAATTCTTGCAATGGCTGAAACGCTACTGTGATTCTGTAAATGGTGGCATTATGAATGAAAACTATAACCCTGTGGAGCGAAGGAGTAAGGGTGGGAAGGATCGGCATCCCAAAGGTGCTCAGAAAATCTCAAAATCTCTGCAATCAAGCAATTTACATAACCCTGGCTCTGGAGATACAGTTGGTCTTAATAAGACCTCAGGACCAAAGCAAGGGAGACCTTATGCAGCTGCAGGCGGTGCTAATTCTTCAGAAGTTCAGGCTTTGTCTAAGGAGATTACAGATCTCAAGCTCTCTGTGGACCTTTTGGAAAAAGAAAGGGACTTTTACTTTGCAAAATTAAGGGATATTGAAATTCTTTGTCAGACATCGGAGTTGGAGGATATCCCGATGTCGGTGGCGGTAAAGAAGATATTGTATGCAGCTGATGCGAAAGAATCACCACTTGCTGAAGCCCAGGAGTACCTCTACCAAACGTTAAATGTCGGCGAAGCCGAAGATGAAACCGAAGCAGAAGCTGAAGCCGAAACAGATACCGGTCATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000079 GO:0000922 GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0005881 GO:0005938 GO:0006355 GO:0008017 GO:0008022 GO:0008092 GO:0008104 GO:0008150 GO:0009524 GO:0009574 GO:0009605 GO:0009606 GO:0009612 GO:0009628 GO:0009629 GO:0009630 GO:0009652 GO:0009889 GO:0009891 GO:0009893 GO:0009958 GO:0010005 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0015630 GO:0015631 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0030496 GO:0030863 GO:0030981 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0033036 GO:0033674 GO:0042325 GO:0042327 GO:0042802 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0048471 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0055028 GO:0060255 GO:0065007 GO:0065009 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1902680 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:2000112 GO:2001141

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

CH EB1

Protein Analysis

286

Amino Acids

31.82

Weight (kDa)

5.32

Isoelectric Point (pI)

47.34

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
CH	PF00307	16 - 114	9.3e-10	Calponin homology (CH) domain
EB1	PF03271	207 - 245	2.7e-16	EB1-like C-terminal motif

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

Font Size (px)

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0013366)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G47690
fragaria_vesca	FvH4_5g15190 FvH4_5g15190
malus_domestica	MD00G1186200.v1.1 MD06G1191400.v1.1 MD14G1197800.v1.1
prunus_persica	Prupe.5G189900_v2.0.a1 Prupe.5G189900_v2.0.a1
pyrus_communis	pycom14g16430
rosa_chinensis	RchiOBHm_Chr7g0180971
rosa_laevigata	RLG00000005220
rosa_multiflora	Rmu_sc0004056.1_g000004
rosa_roxburghii	Rroxscaffold_3G00272370
rosa_rugosa	Rorug06G0438000 Rorug06G0438100
rosa_samantha	Rh7BG038800 Rh7DG039000
rosa_wichuraiana	Rw7G003240

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	222
AciI	CCGC	2 cut(s)	549, 713
AclI	AACGTT	1 cut(s)	788
AclWI	GGATC	3 cut(s)	74, 205, 420
AcsI	RAATTY	2 cut(s)	314, 666
AcuI	CTGAAG	4 cut(s)	65, 546, 783, 852
AfaI	GTAC	1 cut(s)	776
AfiI	CCNNNNNNNGG	4 cut(s)	15, 482, 524, 706
AgeI	ACCGGT	1 cut(s)	848
AgsI	TTSAA	4 cut(s)	104, 244, 272, 665
AjiI	CACGTC	1 cut(s)	63
AjnI	CCWGG	3 cut(s)	154, 475, 768
AluBI	AGCT	5 cut(s)	85, 542, 604, 737, 830
AluI	AGCT	5 cut(s)	85, 542, 604, 737, 830
Alw21I	GWGCWC	1 cut(s)	433
Alw26I	GTCTC	1 cut(s)	523
AlwI	GGATC	3 cut(s)	74, 205, 420
AlwNI	CAGNNNCTG	1 cut(s)	830
AoxI	GGCC	1 cut(s)	296
ApeKI	GCWGC	4 cut(s)	110, 539, 542, 734
ApoI	RAATTY	2 cut(s)	314, 666
ArsI	GACNNNNNNTTYG	2 cut(s)	626, 658
AsiGI	ACCGGT	1 cut(s)	848
AspS9I	GGNCC	2 cut(s)	515, 613
AsuHPI	GGTGA	1 cut(s)	744
AvaII	GGWCC	2 cut(s)	515, 613
AxyI	CCTNAGG	1 cut(s)	511
Bbv12I	GWGCWC	1 cut(s)	433
BbvI	GCAGC	4 cut(s)	97, 529, 551, 746
BccI	CCATC	1 cut(s)	133
BciT130I	CCWGG	3 cut(s)	156, 477, 770
BcoDI	GTCTC	1 cut(s)	523
BfmI	CTRYAG	2 cut(s)	230, 543
BglII	AGATCT	1 cut(s)	595
BisI	GCNGC	4 cut(s)	111, 540, 543, 735
BlsI	GCNGC	4 cut(s)	112, 541, 544, 736
Bme1390I	CCNGG	3 cut(s)	156, 477, 770
Bme18I	GGWCC	2 cut(s)	515, 613
BmgBI	CACGTC	1 cut(s)	63
BmgT120I	GGNCC	2 cut(s)	515, 613
BmrFI	CCNGG	3 cut(s)	156, 477, 770
BmsI	GCATC	5 cut(s)	122, 178, 274, 427, 730
BpmI	CTGGAG	1 cut(s)	504
BpuEI	CTTGAG	1 cut(s)	584
BsaBI	GATNNNNATC	1 cut(s)	417
BsaI	GGTCTC	1 cut(s)	523
BsaJI	CCNNGG	2 cut(s)	475, 768
BsaWI	WCCGGW	1 cut(s)	848
BsaXI	ACNNNNNCTCC	4 cut(s)	388, 418, 477, 507
Bsc4I	CCNNNNNNNGG	4 cut(s)	15, 482, 524, 706
Bse118I	RCCGGY	1 cut(s)	848
Bse21I	CCTNAGG	1 cut(s)	511
Bse3DI	GCAATG	2 cut(s)	134, 329
Bse8I	GATNNNNATC	1 cut(s)	417
BseBI	CCWGG	3 cut(s)	156, 477, 770
BseDI	CCNNGG	2 cut(s)	475, 768
BseGI	GGATG	1 cut(s)	418
BseJI	GATNNNNATC	1 cut(s)	417
BseLI	CCNNNNNNNGG	4 cut(s)	15, 482, 524, 706
BseMI	GCAATG	2 cut(s)	134, 329
BseMII	CTCAG	2 cut(s)	446, 525
BseXI	GCAGC	4 cut(s)	97, 529, 551, 746
BshFI	GGCC	1 cut(s)	298
BshTI	ACCGGT	1 cut(s)	848
BsiHKAI	GWGCWC	1 cut(s)	433
BsiSI	CCGG	1 cut(s)	849
BslFI	GGGAC	1 cut(s)	647
BslI	CCNNNNNNNGG	4 cut(s)	15, 482, 524, 706
BsmAI	GTCTC	1 cut(s)	523
BsmFI	GGGAC	1 cut(s)	647
BsnI	GGCC	1 cut(s)	298
Bso31I	GGTCTC	1 cut(s)	523
Bsp1286I	GDGCHC	1 cut(s)	433
Bsp143I	GATC	4 cut(s)	66, 210, 412, 595
BspACI	CCGC	2 cut(s)	549, 713
BspANI	GGCC	1 cut(s)	298
BspCNI	CTCAG	2 cut(s)	445, 524
BspMAI	CTGCAG	1 cut(s)	547
BspPI	GGATC	3 cut(s)	74, 205, 420
BspTNI	GGTCTC	1 cut(s)	523
BsrDI	GCAATG	2 cut(s)	134, 329
BsrFI	RCCGGY	1 cut(s)	848
BssAI	RCCGGY	1 cut(s)	848
BssECI	CCNNGG	2 cut(s)	475, 768
BssMI	GATC	4 cut(s)	66, 210, 412, 595
Bst2UI	CCWGG	3 cut(s)	156, 477, 770
Bst4CI	ACNGT	3 cut(s)	29, 341, 493
Bst6I	CTCTTC	1 cut(s)	99
BstAPI	GCANNNNNTGC	3 cut(s)	119, 551, 740
BstC8I	GCNNGC	2 cut(s)	296, 547
BstDEI	CTNAG	3 cut(s)	432, 511, 582
BstF5I	GGATG	1 cut(s)	418
BstKTI	GATC	4 cut(s)	69, 213, 415, 598
BstMAI	GTCTC	1 cut(s)	523
BstMBI	GATC	4 cut(s)	66, 210, 412, 595
BstMWI	GCNNNNNNNGC	3 cut(s)	119, 551, 740
BstNI	CCWGG	3 cut(s)	156, 477, 770
BstSCI	CCNGG	3 cut(s)	154, 475, 768
BstSFI	CTRYAG	2 cut(s)	230, 543
BstV1I	GCAGC	4 cut(s)	97, 529, 551, 746
BstX2I	RGATCY	1 cut(s)	595
BstYI	RGATCY	1 cut(s)	595
Bsu36I	CCTNAGG	1 cut(s)	511
BsuRI	GGCC	1 cut(s)	298
BtrI	CACGTC	1 cut(s)	63
BtsCI	GGATG	1 cut(s)	418
BtsIMutI	CAGTG	1 cut(s)	34
Cac8I	GCNNGC	2 cut(s)	296, 547
CaiI	CAGNNNCTG	1 cut(s)	830
Cfr10I	RCCGGY	1 cut(s)	848
Cfr13I	GGNCC	2 cut(s)	515, 613
Csp6I	GTAC	1 cut(s)	775
CspAI	ACCGGT	1 cut(s)	848
CviQI	GTAC	1 cut(s)	775
DdeI	CTNAG	3 cut(s)	432, 511, 582
DpnI	GATC	4 cut(s)	68, 212, 414, 597
DpnII	GATC	4 cut(s)	66, 210, 412, 595
Eam1104I	CTCTTC	1 cut(s)	99
EarI	CTCTTC	1 cut(s)	99
Eco147I	AGGCCT	1 cut(s)	298
Eco31I	GGTCTC	1 cut(s)	523
Eco32I	GATATC	1 cut(s)	697
Eco47I	GGWCC	2 cut(s)	515, 613
Eco57I	CTGAAG	4 cut(s)	65, 546, 783, 852
Eco81I	CCTNAGG	1 cut(s)	511
EcoRI	GAATTC	1 cut(s)	314
EcoRII	CCWGG	3 cut(s)	154, 475, 768
EcoRV	GATATC	1 cut(s)	697
FaqI	GGGAC	1 cut(s)	647
Fnu4HI	GCNGC	4 cut(s)	111, 540, 543, 735
FokI	GGATG	1 cut(s)	405
Fsp4HI	GCNGC	4 cut(s)	111, 540, 543, 735
GluI	GCNGC	4 cut(s)	111, 540, 543, 735
GsuI	CTGGAG	1 cut(s)	504
HaeIII	GGCC	1 cut(s)	298
HapII	CCGG	1 cut(s)	849
HinfI	GANTC	2 cut(s)	344, 749
HpaII	CCGG	1 cut(s)	849
HphI	GGTGA	1 cut(s)	744
Hpy166II	GTNNAC	1 cut(s)	613
Hpy188I	TCNGA	4 cut(s)	435, 565, 678, 685
Hpy188III	TCNNGA	5 cut(s)	214, 269, 483, 513, 700
Hpy8I	GTNNAC	1 cut(s)	613
HpyAV	CCTTC	3 cut(s)	89, 387, 403
HpyCH4III	ACNGT	3 cut(s)	29, 341, 493
HpyCH4IV	ACGT	2 cut(s)	62, 788
HpyCH4V	TGCA	9 cut(s)	113, 127, 172, 322, 454, 539, 545, 647, 734
HpyF10VI	GCNNNNNNNGC	3 cut(s)	119, 551, 740
HpyF3I	CTNAG	3 cut(s)	432, 511, 582
HpySE526I	ACGT	2 cut(s)	62, 788
Kzo9I	GATC	4 cut(s)	66, 210, 412, 595
LmnI	GCTCC	1 cut(s)	388
Lsp1109I	GCAGC	4 cut(s)	97, 529, 551, 746
LweI	GCATC	5 cut(s)	122, 178, 274, 427, 730
MaeII	ACGT	2 cut(s)	62, 788
MalI	GATC	4 cut(s)	68, 212, 414, 597
MboI	GATC	4 cut(s)	66, 210, 412, 595
MboII	GAAGA	4 cut(s)	116, 552, 733, 821
MflI	RGATCY	1 cut(s)	595
MhlI	GDGCHC	1 cut(s)	433
MluCI	AATT	8 cut(s)	181, 217, 255, 314, 463, 556, 650, 666
MmeI	TCCRAC	2 cut(s)	33, 669
MnlI	CCTC	6 cut(s)	39, 100, 101, 520, 685, 788
MseI	TTAA	5 cut(s)	251, 288, 501, 653, 791
MspA1I	CMGCKG	3 cut(s)	85, 542, 737
MspI	CCGG	1 cut(s)	849
MspR9I	CCNGG	3 cut(s)	156, 477, 770
MvaI	CCWGG	3 cut(s)	156, 477, 770
MwoI	GCNNNNNNNGC	3 cut(s)	119, 551, 740
NdeII	GATC	4 cut(s)	66, 210, 412, 595
PceI	AGGCCT	1 cut(s)	298
PcsI	WCGNNNNNNNCGW	1 cut(s)	804
PfeI	GAWTC	2 cut(s)	344, 749
PinAI	ACCGGT	1 cut(s)	848
PkrI	GCNGC	4 cut(s)	112, 541, 544, 736
PsiI	TTATAA	1 cut(s)	222
Psp1406I	AACGTT	1 cut(s)	788
Psp6I	CCWGG	3 cut(s)	154, 475, 768
PspGI	CCWGG	3 cut(s)	154, 475, 768
PspPI	GGNCC	2 cut(s)	515, 613
PstI	CTGCAG	1 cut(s)	547
PstNI	CAGNNNCTG	1 cut(s)	830
PsuI	RGATCY	1 cut(s)	595
PvuII	CAGCTG	3 cut(s)	85, 542, 737
RsaI	GTAC	1 cut(s)	776
RsaNI	GTAC	1 cut(s)	775
SaqAI	TTAA	5 cut(s)	251, 288, 501, 653, 791
SatI	GCNGC	4 cut(s)	111, 540, 543, 735
Sau3AI	GATC	4 cut(s)	66, 210, 412, 595
Sau96I	GGNCC	2 cut(s)	515, 613
ScrFI	CCNGG	3 cut(s)	156, 477, 770
SduI	GDGCHC	1 cut(s)	433
SfaNI	GCATC	5 cut(s)	122, 178, 274, 427, 730
SfcI	CTRYAG	2 cut(s)	230, 543
SinI	GGWCC	2 cut(s)	515, 613
SmlI	CTYRAG	1 cut(s)	599
SmoI	CTYRAG	1 cut(s)	599
Sse9I	AATT	8 cut(s)	181, 217, 255, 314, 463, 556, 650, 666
SseBI	AGGCCT	1 cut(s)	298
SsiI	CCGC	2 cut(s)	549, 713
StuI	AGGCCT	1 cut(s)	298
StyD4I	CCNGG	3 cut(s)	154, 475, 768
TaaI	ACNGT	3 cut(s)	29, 341, 493
TaiI	ACGT	2 cut(s)	65, 791
TasI	AATT	8 cut(s)	181, 217, 255, 314, 463, 556, 650, 666
TfiI	GAWTC	2 cut(s)	344, 749
Tru1I	TTAA	5 cut(s)	251, 288, 501, 653, 791
Tru9I	TTAA	5 cut(s)	251, 288, 501, 653, 791
TscAI	CASTG	1 cut(s)	34
TseI	GCWGC	4 cut(s)	110, 539, 542, 734
TspDTI	ATGAA	3 cut(s)	380, 384, 828
TspRI	CASTG	1 cut(s)	34
VpaK11BI	GGWCC	2 cut(s)	515, 613
XapI	RAATTY	2 cut(s)	314, 666

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.