MD06G1191400.v1.1

Microtubule-associated protein RP EB family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr06
OG0013366
Physical Location & Seq
Forward (+)
32729894 .. 32733621
3728 bp
Loading structure...
UTR
Exon/CDS
Intron
MD06G1191400.v1.1.491

Sequence Viewer

Length: 873 bp
ATGGCTTCCAACATTGGGATAATGGACAGTGCTTACTTTGTTGGGAGGAATGAGATTTTGACGTGGATCAACAATCGCCTTCAGCTCAATCTCTCCCGCATTGAAGAGGCTGCATCTGGTGCTGTGCAATGTCAAATGATGGATATGACCTATCCTGGTGTTGTTCCAATGCACAAGGTCAATTTTGATGCGAAAACAGAATATGATATGATCCAGAATTATAAGGTTCTACAGGAAGTATTCAACAAGTTAAAAATTGAGAAGCCTCTTGAAGTCAATAGGCTTGTTAAAGGCAGGCCTTTGGACAACTTGGAATTCTTGCAATGGCTGAAACGTTACTGTGATTCCATAAATGGTGGCATTATGAATGAAAACTATAACCCTGTGGAGCGAAGGTGCAAGGGTGGGAAGGATCGGCATCCCAAAGGTGCTCAGAAAATCTCAAAATCTCTGCAAGCAAGCAATTTACATAACCCTAGCACTGGAGATACAGTTGGTCTTAATAAGACCTCAGTACAAAGGCAAGGGAAGCCTTATGCAGCAGCAGGCAGTGCTAATTCTTCAGGAGAGATTCAAGCTTTGTCTAAGGAGATTACAGATCTCAAGCTCTCTGTGGACCTTTTGGAAAAAGAAAGGGACTTTTACTTCGCAAAATTAAGGGATATAGAAGTTCTTTGTCAGACATCAGAGTTGGAGGAGGTTCCGATAGCGGTGGCAGTAAAGAAGATATTGTATGCCGCGGATGCAAAAGAATCACCACTTGCTGAAGCCCAGGAGTACCTCTACCAATCCCTGAATGTCGGTGAAGCTGAAGATGAAGGCGAAACCAAAGCTGAAGCAGAAGCAGAAGCCGAAACAGAAACTGACCATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000079 GO:0000922 GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0005881 GO:0005938 GO:0006355 GO:0008017 GO:0008022 GO:0008092 GO:0008104 GO:0008150 GO:0009524 GO:0009574 GO:0009605 GO:0009606 GO:0009612 GO:0009628 GO:0009629 GO:0009630 GO:0009652 GO:0009889 GO:0009891 GO:0009893 GO:0009958 GO:0010005 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0015630 GO:0015631 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0030496 GO:0030863 GO:0030981 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0033036 GO:0033674 GO:0042325 GO:0042327 GO:0042802 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0048471 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0055028 GO:0060255 GO:0065007 GO:0065009 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1902680 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CH EB1

Protein Analysis

291

Amino Acids

32.47

Weight (kDa)

5.12

Isoelectric Point (pI)

43.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 16 - 114 5.3e-10 Calponin homology (CH) domain
EB1 PF03271 208 - 246 2.4e-16 EB1-like C-terminal motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0013366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47690
fragaria_vesca FvH4_5g15190 FvH4_5g15190
malus_domestica MD00G1186200.v1.1 MD06G1191400.v1.1 MD14G1197800.v1.1
prunus_persica Prupe.5G189900_v2.0.a1 Prupe.5G189900_v2.0.a1
pyrus_communis pycom14g16430
rosa_chinensis RchiOBHm_Chr7g0180971
rosa_laevigata RLG00000005220
rosa_multiflora Rmu_sc0004056.1_g000004
rosa_roxburghii Rroxscaffold_3G00272370
rosa_rugosa Rorug06G0438000 Rorug06G0438100
rosa_samantha Rh7BG038800 Rh7DG039000
rosa_wichuraiana Rw7G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 222
AccII CGCG 1 cut(s) 740
AciI CCGC 4 cut(s) 97, 710, 738, 740
AclI AACGTT 1 cut(s) 334
AclWI GGATC 3 cut(s) 74, 205, 420
AcsI RAATTY 1 cut(s) 314
AcuI CTGAAG 5 cut(s) 65, 546, 786, 831, 855
AfaI GTAC 2 cut(s) 516, 779
AfiI CCNNNNNNNGG 2 cut(s) 15, 482
AgsI TTSAA 4 cut(s) 104, 244, 272, 575
AjiI CACGTC 1 cut(s) 63
AjnI CCWGG 2 cut(s) 154, 771
AluBI AGCT 5 cut(s) 85, 578, 607, 809, 833
AluI AGCT 5 cut(s) 85, 578, 607, 809, 833
Alw21I GWGCWC 1 cut(s) 433
AlwI GGATC 3 cut(s) 74, 205, 420
AlwNI CAGNNNCTG 1 cut(s) 863
AoxI GGCC 1 cut(s) 296
ApeKI GCWGC 3 cut(s) 110, 539, 542
ApoI RAATTY 1 cut(s) 314
ArsI GACNNNNNNTTYG 2 cut(s) 629, 661
AspS9I GGNCC 1 cut(s) 616
AsuHPI GGTGA 2 cut(s) 747, 815
AvaII GGWCC 1 cut(s) 616
Bbv12I GWGCWC 1 cut(s) 433
BbvI GCAGC 3 cut(s) 97, 551, 554
BccI CCATC 1 cut(s) 133
BciT130I CCWGG 2 cut(s) 156, 773
BfaI CTAG 1 cut(s) 477
BfmI CTRYAG 1 cut(s) 230
BglII AGATCT 1 cut(s) 598
BisI GCNGC 4 cut(s) 111, 540, 543, 738
BlsI GCNGC 4 cut(s) 112, 541, 544, 739
Bme1390I CCNGG 2 cut(s) 156, 773
Bme18I GGWCC 1 cut(s) 616
BmgBI CACGTC 1 cut(s) 63
BmgT120I GGNCC 1 cut(s) 616
BmiI GGNNCC 1 cut(s) 702
BmrFI CCNGG 2 cut(s) 156, 773
BmsI GCATC 4 cut(s) 122, 178, 427, 733
BpmI CTGGAG 1 cut(s) 504
BpuEI CTTGAG 1 cut(s) 587
BsaBI GATNNNNATC 1 cut(s) 417
BsaJI CCNNGG 2 cut(s) 738, 771
BsaXI ACNNNNNCTCC 4 cut(s) 380, 410, 477, 507
Bsc4I CCNNNNNNNGG 2 cut(s) 15, 482
Bse1I ACTGG 1 cut(s) 487
Bse3DI GCAATG 2 cut(s) 134, 329
Bse8I GATNNNNATC 1 cut(s) 417
BseBI CCWGG 2 cut(s) 156, 773
BseDI CCNNGG 2 cut(s) 738, 771
BseGI GGATG 2 cut(s) 418, 748
BseJI GATNNNNATC 1 cut(s) 417
BseLI CCNNNNNNNGG 2 cut(s) 15, 482
BseMI GCAATG 2 cut(s) 134, 329
BseMII CTCAG 2 cut(s) 446, 525
BseNI ACTGG 1 cut(s) 487
BseRI GAGGAG 1 cut(s) 710
BseXI GCAGC 3 cut(s) 97, 551, 554
Bsh1236I CGCG 1 cut(s) 740
BshFI GGCC 1 cut(s) 298
BsiHKAI GWGCWC 1 cut(s) 433
BslFI GGGAC 1 cut(s) 650
BslI CCNNNNNNNGG 2 cut(s) 15, 482
BsmFI GGGAC 1 cut(s) 650
BsnI GGCC 1 cut(s) 298
Bsp1286I GDGCHC 1 cut(s) 433
Bsp143I GATC 4 cut(s) 66, 210, 412, 598
BspACI CCGC 4 cut(s) 97, 710, 738, 740
BspANI GGCC 1 cut(s) 298
BspCNI CTCAG 2 cut(s) 445, 524
BspFNI CGCG 1 cut(s) 740
BspLI GGNNCC 1 cut(s) 702
BspPI GGATC 3 cut(s) 74, 205, 420
BsrDI GCAATG 2 cut(s) 134, 329
BsrI ACTGG 1 cut(s) 487
BssECI CCNNGG 2 cut(s) 738, 771
BssMI GATC 4 cut(s) 66, 210, 412, 598
Bst2UI CCWGG 2 cut(s) 156, 773
Bst4CI ACNGT 3 cut(s) 29, 341, 493
Bst6I CTCTTC 1 cut(s) 99
BstAPI GCANNNNNTGC 2 cut(s) 119, 551
BstC8I GCNNGC 4 cut(s) 296, 456, 460, 547
BstDEI CTNAG 3 cut(s) 432, 511, 585
BstDSI CCRYGG 1 cut(s) 738
BstF5I GGATG 2 cut(s) 418, 748
BstFNI CGCG 1 cut(s) 740
BstKTI GATC 4 cut(s) 69, 213, 415, 601
BstMBI GATC 4 cut(s) 66, 210, 412, 598
BstMWI GCNNNNNNNGC 4 cut(s) 119, 529, 551, 743
BstNI CCWGG 2 cut(s) 156, 773
BstSCI CCNGG 2 cut(s) 154, 771
BstSFI CTRYAG 1 cut(s) 230
BstUI CGCG 1 cut(s) 740
BstV1I GCAGC 3 cut(s) 97, 551, 554
BstX2I RGATCY 1 cut(s) 598
BstYI RGATCY 1 cut(s) 598
BsuRI GGCC 1 cut(s) 298
BtgI CCRYGG 1 cut(s) 738
BtrI CACGTC 1 cut(s) 63
BtsCI GGATG 2 cut(s) 418, 748
BtsI GCAGTG 1 cut(s) 556
BtsIMutI CAGTG 3 cut(s) 34, 480, 556
Cac8I GCNNGC 4 cut(s) 296, 456, 460, 547
CaiI CAGNNNCTG 1 cut(s) 863
Cfr13I GGNCC 1 cut(s) 616
Cfr42I CCGCGG 1 cut(s) 741
Csp6I GTAC 2 cut(s) 515, 778
CviQI GTAC 2 cut(s) 515, 778
DdeI CTNAG 3 cut(s) 432, 511, 585
DpnI GATC 4 cut(s) 68, 212, 414, 600
DpnII GATC 4 cut(s) 66, 210, 412, 598
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 298
Eco47I GGWCC 1 cut(s) 616
Eco57I CTGAAG 5 cut(s) 65, 546, 786, 831, 855
EcoRI GAATTC 1 cut(s) 314
EcoRII CCWGG 2 cut(s) 154, 771
FaqI GGGAC 1 cut(s) 650
FauI CCCGC 1 cut(s) 104
Fnu4HI GCNGC 4 cut(s) 111, 540, 543, 738
FokI GGATG 2 cut(s) 405, 755
Fsp4HI GCNGC 4 cut(s) 111, 540, 543, 738
FspBI CTAG 1 cut(s) 477
GluI GCNGC 4 cut(s) 111, 540, 543, 738
GsuI CTGGAG 1 cut(s) 504
HaeIII GGCC 1 cut(s) 298
HindIII AAGCTT 1 cut(s) 576
HinfI GANTC 3 cut(s) 344, 571, 752
HphI GGTGA 2 cut(s) 747, 815
Hpy166II GTNNAC 1 cut(s) 616
Hpy188I TCNGA 4 cut(s) 435, 681, 688, 705
Hpy188III TCNNGA 3 cut(s) 214, 269, 564
Hpy8I GTNNAC 1 cut(s) 616
HpyAV CCTTC 4 cut(s) 89, 387, 403, 812
HpyCH4III ACNGT 3 cut(s) 29, 341, 493
HpyCH4IV ACGT 2 cut(s) 62, 334
HpyCH4V TGCA 8 cut(s) 113, 127, 172, 322, 399, 454, 539, 746
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 529, 551, 743
HpyF3I CTNAG 3 cut(s) 432, 511, 585
HpySE526I ACGT 2 cut(s) 62, 334
KspI CCGCGG 1 cut(s) 741
Kzo9I GATC 4 cut(s) 66, 210, 412, 598
LmnI GCTCC 1 cut(s) 388
Lsp1109I GCAGC 3 cut(s) 97, 551, 554
LweI GCATC 4 cut(s) 122, 178, 427, 733
MaeI CTAG 1 cut(s) 477
MaeII ACGT 2 cut(s) 62, 334
MaeIII GTNAC 1 cut(s) 335
MalI GATC 4 cut(s) 68, 212, 414, 600
MboI GATC 4 cut(s) 66, 210, 412, 598
MboII GAAGA 4 cut(s) 116, 552, 736, 824
MflI RGATCY 1 cut(s) 598
MhlI GDGCHC 1 cut(s) 433
MluCI AATT 7 cut(s) 181, 217, 255, 314, 463, 556, 653
MmeI TCCRAC 2 cut(s) 33, 672
MnlI CCTC 7 cut(s) 39, 100, 276, 520, 688, 691, 791
MseI TTAA 4 cut(s) 251, 288, 501, 656
MspA1I CMGCKG 1 cut(s) 740
MspR9I CCNGG 2 cut(s) 156, 773
MvaI CCWGG 2 cut(s) 156, 773
MvnI CGCG 1 cut(s) 740
MwoI GCNNNNNNNGC 4 cut(s) 119, 529, 551, 743
NdeII GATC 4 cut(s) 66, 210, 412, 598
NlaIV GGNNCC 1 cut(s) 702
PceI AGGCCT 1 cut(s) 298
PfeI GAWTC 3 cut(s) 344, 571, 752
PkrI GCNGC 4 cut(s) 112, 541, 544, 739
PsiI TTATAA 1 cut(s) 222
Psp1406I AACGTT 1 cut(s) 334
Psp6I CCWGG 2 cut(s) 154, 771
PspGI CCWGG 2 cut(s) 154, 771
PspN4I GGNNCC 1 cut(s) 702
PspPI GGNCC 1 cut(s) 616
PstNI CAGNNNCTG 1 cut(s) 863
PsuI RGATCY 1 cut(s) 598
RsaI GTAC 2 cut(s) 516, 779
RsaNI GTAC 2 cut(s) 515, 778
SacII CCGCGG 1 cut(s) 741
SaqAI TTAA 4 cut(s) 251, 288, 501, 656
SatI GCNGC 4 cut(s) 111, 540, 543, 738
Sau3AI GATC 4 cut(s) 66, 210, 412, 598
Sau96I GGNCC 1 cut(s) 616
ScrFI CCNGG 2 cut(s) 156, 773
SduI GDGCHC 1 cut(s) 433
SfaNI GCATC 4 cut(s) 122, 178, 427, 733
SfcI CTRYAG 1 cut(s) 230
Sfr303I CCGCGG 1 cut(s) 741
SgrBI CCGCGG 1 cut(s) 741
SinI GGWCC 1 cut(s) 616
SmlI CTYRAG 1 cut(s) 602
SmoI CTYRAG 1 cut(s) 602
Sse9I AATT 7 cut(s) 181, 217, 255, 314, 463, 556, 653
SseBI AGGCCT 1 cut(s) 298
SsiI CCGC 4 cut(s) 97, 710, 738, 740
SspMI CTAG 1 cut(s) 477
StuI AGGCCT 1 cut(s) 298
StyD4I CCNGG 2 cut(s) 154, 771
TaaI ACNGT 3 cut(s) 29, 341, 493
TaiI ACGT 2 cut(s) 65, 337
TasI AATT 7 cut(s) 181, 217, 255, 314, 463, 556, 653
TatI WGTACW 1 cut(s) 514
TauI GCSGC 1 cut(s) 740
TfiI GAWTC 3 cut(s) 344, 571, 752
Tru1I TTAA 4 cut(s) 251, 288, 501, 656
Tru9I TTAA 4 cut(s) 251, 288, 501, 656
TscAI CASTG 3 cut(s) 34, 487, 556
TseI GCWGC 3 cut(s) 110, 539, 542
TspDTI ATGAA 3 cut(s) 380, 384, 831
TspRI CASTG 3 cut(s) 34, 487, 556
VpaK11BI GGWCC 1 cut(s) 616
XapI RAATTY 1 cut(s) 314
XspI CTAG 1 cut(s) 477
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.