Rw7G003240

Microtubule-associated protein RP EB family member

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0013366
Physical Location & Seq
Forward (+)
2620559 .. 2623544
2986 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G003240.1

Sequence Viewer

Length: 831 bp
ATGGCTTCCAACATAGGAATTATGGACAGTGCTTACTTCGTTGGAAGGAATGAGATTTTGACTTGGATCAACAATCGTCTTCAGCTCAATCTCTCTCGCATCGAAGAGGCTGCATCTGGTGCTGTGCAATGCCAAATGATGGATATGACGTACCCAGGAGTTGTTCCAATGCACAAGGTCAATTTTGATGCGAAGACAGAATATGATATGATCCAGAACTATAAGATTTTACAGGAGGTGTTCAATAAGTTAAAAATTGAGAAGCCTCTTGAAGTCAATAGGCTTGTTAAAGGCAGGCCTTTGGACAACTTGGAGTTCTTACAATGGCTGAAACGATACTGTGATTCTGTCAATGGTGGCATTATGAATGAAAATTATAATCCTGTTGAGCGAAGGTGCAAGGGAGGGAAGGAACGGTATGCTAAGGGTTCTCAAAAAATCCCAAAATCACTGCAGGCAAACAATTCAGGTGACCCAGTTGGTCTTAGCAGAACCTCTGGACCAAAGCAAGGGAAGACTTATGCAGGGGCAGGTGGTGCTAATTACGAAGGAGAGGTCCTAGCTTTGTCTAAGGAGATAACAGATCTCAAGGTCTCGGTGGACCTTTTGGAAAAAGAAAGAGACTTTTACTTTGGAAAACTGAGGGATATTGAAATTCTTTGTCAAACGTCTGGATTGGAGAGTATCCCGATGTCAGTAGCAATAAAGAAGATATTGTATGCTGCTGATGCAAAAGAATCAGCTCTTGCCAAAGCTCAAGAATATCTCCTTTCCGTGGAAGAAGATGAAATTGAAGCAGGAGCTGGAGCTGAAGCTGAAACAGATCCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000079 GO:0000922 GO:0001932 GO:0001934 GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005737 GO:0005815 GO:0005819 GO:0005856 GO:0005874 GO:0005876 GO:0005881 GO:0005938 GO:0006355 GO:0008017 GO:0008022 GO:0008092 GO:0008104 GO:0008150 GO:0009524 GO:0009574 GO:0009605 GO:0009606 GO:0009612 GO:0009628 GO:0009629 GO:0009630 GO:0009652 GO:0009889 GO:0009891 GO:0009893 GO:0009958 GO:0010005 GO:0010468 GO:0010556 GO:0010557 GO:0010562 GO:0010604 GO:0010628 GO:0015630 GO:0015631 GO:0019219 GO:0019220 GO:0019222 GO:0019899 GO:0019900 GO:0019901 GO:0030496 GO:0030863 GO:0030981 GO:0031323 GO:0031325 GO:0031326 GO:0031328 GO:0031399 GO:0031401 GO:0032268 GO:0032270 GO:0033036 GO:0033674 GO:0042325 GO:0042327 GO:0042802 GO:0043085 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043549 GO:0044093 GO:0044422 GO:0044424 GO:0044430 GO:0044444 GO:0044446 GO:0044448 GO:0044464 GO:0045737 GO:0045787 GO:0045859 GO:0045860 GO:0045893 GO:0045935 GO:0045937 GO:0048471 GO:0048518 GO:0048522 GO:0050789 GO:0050790 GO:0050794 GO:0050896 GO:0051171 GO:0051173 GO:0051174 GO:0051179 GO:0051246 GO:0051247 GO:0051252 GO:0051254 GO:0051338 GO:0051347 GO:0051726 GO:0055028 GO:0060255 GO:0065007 GO:0065009 GO:0071900 GO:0071902 GO:0071944 GO:0080090 GO:0099080 GO:0099081 GO:0099512 GO:0099513 GO:0099568 GO:1902680 GO:1903506 GO:1903508 GO:1904029 GO:1904031 GO:2000112 GO:2001141
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
CH EB1

Protein Analysis

276

Amino Acids

30.7

Weight (kDa)

5.28

Isoelectric Point (pI)

45.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CH PF00307 16 - 114 5.8e-10 Calponin homology (CH) domain
EB1 PF03271 203 - 241 5.3e-16 EB1-like C-terminal motif
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0013366)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G47690
fragaria_vesca FvH4_5g15190 FvH4_5g15190
malus_domestica MD00G1186200.v1.1 MD06G1191400.v1.1 MD14G1197800.v1.1
prunus_persica Prupe.5G189900_v2.0.a1 Prupe.5G189900_v2.0.a1
pyrus_communis pycom14g16430
rosa_chinensis RchiOBHm_Chr7g0180971
rosa_laevigata RLG00000005220
rosa_multiflora Rmu_sc0004056.1_g000004
rosa_roxburghii Rroxscaffold_3G00272370
rosa_rugosa Rorug06G0438000 Rorug06G0438100
rosa_samantha Rh7BG038800 Rh7DG039000
rosa_wichuraiana Rw7G003240

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 378
AarI CACCTGC 1 cut(s) 521
Acc36I ACCTGC 1 cut(s) 521
AccB7I CCANNNNNTGG 1 cut(s) 139
AclWI GGATC 3 cut(s) 74, 205, 818
AcsI RAATTY 1 cut(s) 654
AcuI CTGAAG 2 cut(s) 65, 831
AfaI GTAC 1 cut(s) 152
AfiI CCNNNNNNNGG 3 cut(s) 139, 509, 775
AgsI TTSAA 4 cut(s) 244, 272, 653, 794
AjnI CCWGG 1 cut(s) 154
AluBI AGCT 7 cut(s) 85, 563, 743, 755, 803, 809, 815
AluI AGCT 7 cut(s) 85, 563, 743, 755, 803, 809, 815
Alw26I GTCTC 2 cut(s) 598, 615
AlwI GGATC 3 cut(s) 74, 205, 818
AlwNI CAGNNNCTG 1 cut(s) 803
AoxI GGCC 1 cut(s) 296
ApeKI GCWGC 2 cut(s) 110, 722
ApoI RAATTY 1 cut(s) 654
ArsI GACNNNNNNTTYG 4 cut(s) 540, 572, 614, 646
AspS9I GGNCC 3 cut(s) 500, 556, 601
AsuHPI GGTGA 1 cut(s) 482
AvaII GGWCC 3 cut(s) 500, 556, 601
BbsI GAAGAC 3 cut(s) 71, 200, 521
BbvI GCAGC 2 cut(s) 97, 709
BccI CCATC 1 cut(s) 133
BcgI CGANNNNNNTGC 2 cut(s) 92, 126
BciT130I CCWGG 1 cut(s) 156
BciVI GTATCC 1 cut(s) 695
BcoDI GTCTC 2 cut(s) 598, 615
BfaI CTAG 1 cut(s) 560
BfmI CTRYAG 1 cut(s) 452
BfuAI ACCTGC 1 cut(s) 521
BfuI GTATCC 1 cut(s) 695
BglII AGATCT 1 cut(s) 583
BisI GCNGC 2 cut(s) 111, 723
BlsI GCNGC 2 cut(s) 112, 724
Bme1390I CCNGG 1 cut(s) 156
Bme18I GGWCC 3 cut(s) 500, 556, 601
BmgT120I GGNCC 3 cut(s) 500, 556, 601
BmrFI CCNGG 1 cut(s) 156
BmrI ACTGGG 1 cut(s) 470
BmsI GCATC 4 cut(s) 108, 122, 178, 718
BmuI ACTGGG 1 cut(s) 470
BpiI GAAGAC 3 cut(s) 71, 200, 521
BpmI CTGGAG 1 cut(s) 825
Bpu10I CCTNAGC 1 cut(s) 423
BpuEI CTTGAG 2 cut(s) 572, 741
BsaI GGTCTC 1 cut(s) 598
BsaJI CCNNGG 2 cut(s) 154, 774
Bsc4I CCNNNNNNNGG 3 cut(s) 139, 509, 775
Bse1I ACTGG 1 cut(s) 476
Bse3DI GCAATG 1 cut(s) 134
BseBI CCWGG 1 cut(s) 156
BseDI CCNNGG 2 cut(s) 154, 774
BseLI CCNNNNNNNGG 3 cut(s) 139, 509, 775
BseMI GCAATG 1 cut(s) 134
BseMII CTCAG 1 cut(s) 632
BseNI ACTGG 1 cut(s) 476
BseXI GCAGC 2 cut(s) 97, 709
BshFI GGCC 1 cut(s) 298
BslI CCNNNNNNNGG 3 cut(s) 139, 509, 775
BsmAI GTCTC 2 cut(s) 598, 615
BsnI GGCC 1 cut(s) 298
Bso31I GGTCTC 1 cut(s) 598
Bsp143I GATC 4 cut(s) 66, 210, 583, 823
BspANI GGCC 1 cut(s) 298
BspCNI CTCAG 1 cut(s) 633
BspMAI CTGCAG 1 cut(s) 456
BspMI ACCTGC 1 cut(s) 521
BspPI GGATC 3 cut(s) 74, 205, 818
BspTNI GGTCTC 1 cut(s) 598
BsrDI GCAATG 1 cut(s) 134
BsrI ACTGG 1 cut(s) 476
BssECI CCNNGG 2 cut(s) 154, 774
BssMI GATC 4 cut(s) 66, 210, 583, 823
Bst2UI CCWGG 1 cut(s) 156
Bst4CI ACNGT 3 cut(s) 29, 341, 417
Bst6I CTCTTC 1 cut(s) 99
BstAPI GCANNNNNTGC 2 cut(s) 119, 536
BstC8I GCNNGC 2 cut(s) 296, 456
BstDEI CTNAG 4 cut(s) 423, 485, 570, 641
BstDSI CCRYGG 1 cut(s) 774
BstEII GGTNACC 1 cut(s) 470
BstKTI GATC 4 cut(s) 69, 213, 586, 826
BstMAI GTCTC 2 cut(s) 598, 615
BstMBI GATC 4 cut(s) 66, 210, 583, 823
BstMWI GCNNNNNNNGC 3 cut(s) 119, 536, 728
BstNI CCWGG 1 cut(s) 156
BstPI GGTNACC 1 cut(s) 470
BstSCI CCNGG 1 cut(s) 154
BstSFI CTRYAG 1 cut(s) 452
BstV1I GCAGC 2 cut(s) 97, 709
BstV2I GAAGAC 3 cut(s) 71, 200, 521
BstX2I RGATCY 2 cut(s) 583, 823
BstYI RGATCY 2 cut(s) 583, 823
BsuI GTATCC 1 cut(s) 695
BsuRI GGCC 1 cut(s) 298
BtgI CCRYGG 1 cut(s) 774
BtsI GCAGTG 1 cut(s) 449
BtsIMutI CAGTG 2 cut(s) 34, 449
BveI ACCTGC 1 cut(s) 521
Cac8I GCNNGC 2 cut(s) 296, 456
CaiI CAGNNNCTG 1 cut(s) 803
Cfr13I GGNCC 3 cut(s) 500, 556, 601
Csp6I GTAC 1 cut(s) 151
CviQI GTAC 1 cut(s) 151
DdeI CTNAG 4 cut(s) 423, 485, 570, 641
DpnI GATC 4 cut(s) 68, 212, 585, 825
DpnII GATC 4 cut(s) 66, 210, 583, 823
Eam1104I CTCTTC 1 cut(s) 99
EarI CTCTTC 1 cut(s) 99
Eco147I AGGCCT 1 cut(s) 298
Eco31I GGTCTC 1 cut(s) 598
Eco47I GGWCC 3 cut(s) 500, 556, 601
Eco57I CTGAAG 2 cut(s) 65, 831
Eco91I GGTNACC 1 cut(s) 470
EcoO109I RGGNCCY 1 cut(s) 556
EcoO65I GGTNACC 1 cut(s) 470
EcoRII CCWGG 1 cut(s) 154
Fnu4HI GCNGC 2 cut(s) 111, 723
Fsp4HI GCNGC 2 cut(s) 111, 723
FspBI CTAG 1 cut(s) 560
GluI GCNGC 2 cut(s) 111, 723
GsuI CTGGAG 1 cut(s) 825
HaeIII GGCC 1 cut(s) 298
HinfI GANTC 2 cut(s) 344, 737
HphI GGTGA 1 cut(s) 482
Hpy166II GTNNAC 1 cut(s) 601
Hpy188III TCNNGA 6 cut(s) 214, 269, 498, 672, 688, 758
Hpy8I GTNNAC 1 cut(s) 601
HpyAV CCTTC 4 cut(s) 39, 387, 403, 542
HpyCH4III ACNGT 3 cut(s) 29, 341, 417
HpyCH4IV ACGT 2 cut(s) 149, 668
HpyCH4V TGCA 7 cut(s) 113, 127, 172, 399, 454, 524, 731
HpyF10VI GCNNNNNNNGC 3 cut(s) 119, 536, 728
HpyF3I CTNAG 4 cut(s) 423, 485, 570, 641
HpySE526I ACGT 2 cut(s) 149, 668
Kzo9I GATC 4 cut(s) 66, 210, 583, 823
LmnI GCTCC 2 cut(s) 800, 806
Lsp1109I GCAGC 2 cut(s) 97, 709
LweI GCATC 4 cut(s) 108, 122, 178, 718
MaeI CTAG 1 cut(s) 560
MaeII ACGT 2 cut(s) 149, 668
MaeIII GTNAC 1 cut(s) 470
MalI GATC 4 cut(s) 68, 212, 585, 825
MboI GATC 4 cut(s) 66, 210, 583, 823
MboII GAAGA 7 cut(s) 71, 116, 205, 526, 721, 791, 794
MflI RGATCY 2 cut(s) 583, 823
MluCI AATT 8 cut(s) 18, 181, 255, 373, 463, 541, 654, 789
MmeI TCCRAC 2 cut(s) 22, 33
MnlI CCTC 7 cut(s) 100, 229, 276, 398, 505, 547, 636
MseI TTAA 2 cut(s) 251, 288
MspR9I CCNGG 1 cut(s) 156
MvaI CCWGG 1 cut(s) 156
MwoI GCNNNNNNNGC 3 cut(s) 119, 536, 728
NdeII GATC 4 cut(s) 66, 210, 583, 823
NmuCI GTSAC 1 cut(s) 470
PaqCI CACCTGC 1 cut(s) 521
PceI AGGCCT 1 cut(s) 298
PfeI GAWTC 2 cut(s) 344, 737
PflMI CCANNNNNTGG 1 cut(s) 139
PkrI GCNGC 2 cut(s) 112, 724
PpuMI RGGWCCY 1 cut(s) 556
PsiI TTATAA 1 cut(s) 378
Psp5II RGGWCCY 1 cut(s) 556
Psp6I CCWGG 1 cut(s) 154
PspEI GGTNACC 1 cut(s) 470
PspGI CCWGG 1 cut(s) 154
PspPI GGNCC 3 cut(s) 500, 556, 601
PspPPI RGGWCCY 1 cut(s) 556
PstI CTGCAG 1 cut(s) 456
PstNI CAGNNNCTG 1 cut(s) 803
PsuI RGATCY 2 cut(s) 583, 823
RsaI GTAC 1 cut(s) 152
RsaNI GTAC 1 cut(s) 151
SaqAI TTAA 2 cut(s) 251, 288
SatI GCNGC 2 cut(s) 111, 723
Sau3AI GATC 4 cut(s) 66, 210, 583, 823
Sau96I GGNCC 3 cut(s) 500, 556, 601
ScrFI CCNGG 1 cut(s) 156
SfaNI GCATC 4 cut(s) 108, 122, 178, 718
SfcI CTRYAG 1 cut(s) 452
SinI GGWCC 3 cut(s) 500, 556, 601
SmlI CTYRAG 2 cut(s) 587, 756
SmoI CTYRAG 2 cut(s) 587, 756
Sse9I AATT 8 cut(s) 18, 181, 255, 373, 463, 541, 654, 789
SseBI AGGCCT 1 cut(s) 298
SspMI CTAG 1 cut(s) 560
StuI AGGCCT 1 cut(s) 298
StyD4I CCNGG 1 cut(s) 154
TaaI ACNGT 3 cut(s) 29, 341, 417
TaiI ACGT 2 cut(s) 152, 671
TaqI TCGA 1 cut(s) 102
TasI AATT 8 cut(s) 18, 181, 255, 373, 463, 541, 654, 789
TfiI GAWTC 2 cut(s) 344, 737
Tru1I TTAA 2 cut(s) 251, 288
Tru9I TTAA 2 cut(s) 251, 288
TscAI CASTG 2 cut(s) 34, 456
TseFI GTSAC 1 cut(s) 470
TseI GCWGC 2 cut(s) 110, 722
Tsp45I GTSAC 1 cut(s) 470
TspDTI ATGAA 3 cut(s) 380, 384, 801
TspGWI ACGGA 2 cut(s) 763, 816
TspRI CASTG 2 cut(s) 34, 456
Van91I CCANNNNNTGG 1 cut(s) 139
VpaK11BI GGWCC 3 cut(s) 500, 556, 601
XapI RAATTY 1 cut(s) 654
XspI CTAG 1 cut(s) 560
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.