MD01G1023900.v1.1

Belongs to the MICOS complex subunit Mic60 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr01
OG0011414
Physical Location & Seq
Forward (+)
9096572 .. 9102685
6114 bp
Loading structure...
UTR
Exon/CDS
Intron
MD01G1023900.v1.1.491

Sequence Viewer

Length: 1842 bp
ATGTTGCGAAGGTCAATTCTGCAGATTGCGTCTCGTCGTGGGGTTAGAAGAAGGATTCCGAGGCCGATTACAAGGGAATTCTCCGCCGCAGCCCCCAAAACTGCTTCCCACCACCCTGGCGATGCAGCCAATCCGCCGCCGCCGGGAAACGGCGGTTCCAGCGCCAAAATTTTCCTCGGAAGCGCTGCCGTTGGTGCTGGGCTTGTGGCAGCTTACAAAACCGGGTATCTAGACCCAATTTTTGGTGCCAAAGAGAAGAGTGATTCGGTTAAGGATGCTGCCATTGGTTTGGAGGATCACAAACATGTAGAAGAAGAAAAACATGATGAAGAAAGAGCTGGCATTGCCGAGGAGAAGGTCGAAACGCGTTCGGATGTTCCCAATGTTGAGGGTTTGAGTGGAAGCGATGGCGAAAACCCGCAGTCTCAAGTGGATGAGGATAATAAAGCTGAGAGTGACATCACAAGTGAGAAAGGTTTGCCTGAGCATTCCCAAAATATAAATCTTGGGTCATCATCTGAAGGGAATGTAGACGTGAAGAGTGCAGGGGAAAAAACTAGTGTGGAGATAAATGAGGCGCCCGTAGTTTCCCAGACTAGTGCAGATCAGCAAGAGAAGGAGGCGAAAACACCGCCACCTCAACCGGATATTGTACAAGACAAGGCAGAGGTTGCATTAGGTATTGATGAAGAGCCATCAGGTTCTCTCCTCAAGGCATATCATCTGAAGGACGAGGTCGATGAAGGCATCAAAATTACTAATAGGGAGGATGGTATTGAATACAATAACTTTCCTAATGAAAAAGAGGCTCTCGATGCAATCGAAGGGCTAAATGATGCGTACATATCTAAAGATGGAAAGTTAGTTCTCGACTTCTTACAAACCATTCATACTGCTGAGAGAAGGCAAGCGGAGTTAGATGCTCATGTTTTTGCAGAAGAAAAGAGAATGTTAAAGGAGAAGTATGAAAAGAAACTAAAGGATGCTGCGGCCAGGGAATTGATGCTCGCAGAAGAGGCAGCAATGCTAGACAAGGAATTAAAGAGAGAGAGGGCAAAAGCAGCTGCTGCACTCAAGTCACTTCAAGAAAAGCTTGAAGAGAAATATAAGACAGAACTTGAGCACAAGGAAAGTGAAGCTGAAATGAAGTTAAAGAAGGTTGAGGAATTAGCAAAAGCGGGACTGGCAGCAACAATTGCAAGAGAGAAGGCATCCCAAATTGAGAAAATGGCAGAAGCAAACCTTCATATAAATGCCTTGTGTGTGGCATTCTATGCAAGATCTGAAGAAGCTCGTCAGACTCACTCTGCTCACAAATTTGCTTTGGGAGCACTTGCACTAGATGATGCACTTTCAAAAGGATTGCCAATTGAGAGAGAAATAGAGGCTTTGCACACTTACCTTGAAGGCATTGATAAAGATTCAATTTTGGATGTGGTCCTATCATCTCTTCCTGAAGAAACACGAAGGAATGGCACAGATACCTTATTGCAATTGAATCAGAAGTTTGATGCCTTGAAAGGGACAGTGCGGCACCTCAGCTTAATCCCTCTTGGTGGTGGTGGCATTTTGGCACATTCCTTGGCACATATTGCATCCTGGTTGAAGGTGAAGGAAGTGGACCACTCAGGGGACGGAATTGAGTCCATTATCAACAAAGTGGAGTACTACCTAGCTGAAGGAAAGATAGCCGAAGCAGCAGAGGCACTTGAAGAAGGAGTGAAAGGCACCCAAGCAATGGGAGTAGTGAGTGAATGGGTGAAGCGTGCTAGGAATAGGGCTATCACAGACCAAGCTCTAACGCTTCTCCAATCATATGCTACTTCTATCAGCGTTACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mitofilin

Protein Analysis

614

Amino Acids

66.76

Weight (kDa)

5.2

Isoelectric Point (pI)

47.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mitofilin PF09731 100 - 608 5.8e-69 Mitochondrial inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39690
fragaria_vesca FvH4_1g21670 FvH4_1g21670 FvH4_1g21670
malus_domestica MD01G1023900.v1.1 MD15G1329400.v1.1
prunus_persica Prupe.6G178900_v2.0.a1
pyrus_communis pycom01g05150
rosa_chinensis RchiOBHm_Chr2g0115541
rosa_laevigata RLG00000018192
rosa_multiflora Rmu_ssc0000197.1_g000091
rosa_roxburghii Rroxscaffold_2G00128120 Rroxscaffold_2G00128990
rosa_rugosa Rorug02G0197700 Rorug02G0197800
rosa_samantha Rh2AG255100 Rh2BG266000 Rh2CG260300 Rh2DG263300
rosa_wichuraiana Rw2G019970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 4 cut(s) 245, 577, 1533, 1727
AccB7I CCANNNNNTGG 2 cut(s) 242, 1738
AccI GTMKAC 1 cut(s) 531
AccII CGCG 1 cut(s) 367
AclWI GGATC 1 cut(s) 303
AcoI YGGCCR 1 cut(s) 990
AcsI RAATTY 3 cut(s) 77, 168, 1316
AcuI CTGAAG 5 cut(s) 540, 746, 1305, 1476, 1698
AcyI GRCGYC 1 cut(s) 578
AfaI GTAC 3 cut(s) 654, 842, 1667
AfeI AGCGCT 1 cut(s) 184
AfiI CCNNNNNNNGG 7 cut(s) 143, 149, 242, 1521, 1556, 1630, 1738
AflIII ACRYGT 2 cut(s) 304, 365
AhlI ACTAGT 2 cut(s) 557, 596
AjiI CACGTC 1 cut(s) 535
AjnI CCWGG 3 cut(s) 115, 992, 1598
AloI GAACNNNNNNTCC 2 cut(s) 849, 881
Alw21I GWGCWC 2 cut(s) 1125, 1333
Alw26I GTCTC 2 cut(s) 36, 429
AlwI GGATC 1 cut(s) 303
AlwNI CAGNNNCTG 1 cut(s) 1067
Aor51HI AGCGCT 1 cut(s) 184
AoxI GGCC 2 cut(s) 62, 990
ApoI RAATTY 3 cut(s) 77, 168, 1316
ArsI GACNNNNNNTTYG 2 cut(s) 224, 256
AspLEI GCGC 3 cut(s) 164, 185, 580
AspS9I GGNCC 2 cut(s) 1438, 1621
AsuC2I CCSGG 2 cut(s) 144, 223
AsuHPI GGTGA 2 cut(s) 1621, 1771
AvaII GGWCC 2 cut(s) 1438, 1621
BanI GGYRCC 4 cut(s) 245, 577, 1533, 1727
Bbv12I GWGCWC 2 cut(s) 1125, 1333
BbvCI CCTCAGC 1 cut(s) 1538
BccI CCATC 4 cut(s) 401, 703, 764, 848
BceAI ACGGC 2 cut(s) 166, 173
BciT130I CCWGG 3 cut(s) 117, 994, 1600
BcnI CCSGG 2 cut(s) 144, 223
BcoDI GTCTC 2 cut(s) 36, 429
BcuI ACTAGT 2 cut(s) 557, 596
BfaI CTAG 7 cut(s) 230, 558, 597, 1028, 1340, 1673, 1770
BfmI CTRYAG 1 cut(s) 20
BfoI RGCGCY 3 cut(s) 165, 186, 581
BglII AGATCT 1 cut(s) 1280
BmcAI AGTACT 1 cut(s) 1667
Bme1390I CCNGG 5 cut(s) 117, 144, 223, 994, 1600
Bme18I GGWCC 2 cut(s) 1438, 1621
BmgBI CACGTC 1 cut(s) 535
BmgT120I GGNCC 2 cut(s) 1438, 1621
BmiI GGNNCC 5 cut(s) 157, 247, 579, 1535, 1729
BmrFI CCNGG 5 cut(s) 117, 144, 223, 994, 1600
Bpu10I CCTNAGC 2 cut(s) 483, 1538
BpuEI CTTGAG 4 cut(s) 411, 695, 1058, 1139
BpuMI CCSGG 2 cut(s) 144, 223
BsaHI GRCGYC 1 cut(s) 578
BsaJI CCNNGG 6 cut(s) 59, 115, 175, 348, 993, 1581
BsaWI WCCGGW 1 cut(s) 643
Bsc4I CCNNNNNNNGG 7 cut(s) 143, 149, 242, 1521, 1556, 1630, 1738
Bse1I ACTGG 1 cut(s) 1188
Bse3DI GCAATG 3 cut(s) 342, 1029, 1743
BseBI CCWGG 3 cut(s) 117, 994, 1600
BseDI CCNNGG 6 cut(s) 59, 115, 175, 348, 993, 1581
BseGI GGATG 8 cut(s) 280, 379, 439, 775, 988, 1211, 1438, 1595
BseLI CCNNNNNNNGG 7 cut(s) 143, 149, 242, 1521, 1556, 1630, 1738
BseMI GCAATG 3 cut(s) 342, 1029, 1743
BseMII CTCAG 5 cut(s) 441, 474, 888, 1552, 1641
BseNI ACTGG 1 cut(s) 1188
BseRI GAGGAG 2 cut(s) 365, 698
BseYI CCCAGC 1 cut(s) 197
BsgI GTGCAG 3 cut(s) 564, 621, 1053
Bsh1236I CGCG 1 cut(s) 367
BshFI GGCC 2 cut(s) 64, 992
BshNI GGYRCC 4 cut(s) 245, 577, 1533, 1727
BsiHKAI GWGCWC 2 cut(s) 1125, 1333
BsiSI CCGG 3 cut(s) 143, 222, 644
BslFI GGGAC 3 cut(s) 1194, 1537, 1646
BslI CCNNNNNNNGG 7 cut(s) 143, 149, 242, 1521, 1556, 1630, 1738
BsmAI GTCTC 2 cut(s) 36, 429
BsmBI CGTCTC 1 cut(s) 36
BsmFI GGGAC 3 cut(s) 1194, 1537, 1646
BsmI GAATGC 2 cut(s) 487, 1268
BsnI GGCC 2 cut(s) 64, 992
Bsp1286I GDGCHC 2 cut(s) 1125, 1333
Bsp1407I TGTACA 1 cut(s) 652
Bsp143I GATC 3 cut(s) 295, 604, 1280
BspANI GGCC 2 cut(s) 64, 992
BspCNI CTCAG 5 cut(s) 442, 475, 889, 1551, 1640
BspFNI CGCG 1 cut(s) 367
BspLI GGNNCC 5 cut(s) 157, 247, 579, 1535, 1729
BspMAI CTGCAG 1 cut(s) 24
BspPI GGATC 1 cut(s) 303
BspQI GCTCTTC 1 cut(s) 684
BspT107I GGYRCC 4 cut(s) 245, 577, 1533, 1727
BsrDI GCAATG 3 cut(s) 342, 1029, 1743
BsrGI TGTACA 1 cut(s) 652
BsrI ACTGG 1 cut(s) 1188
BssECI CCNNGG 6 cut(s) 59, 115, 175, 348, 993, 1581
BssMI GATC 3 cut(s) 295, 604, 1280
BssNI GRCGYC 1 cut(s) 578
BssT1I CCWWGG 1 cut(s) 1581
Bst2UI CCWGG 3 cut(s) 117, 994, 1600
Bst4CI ACNGT 1 cut(s) 1528
Bst6I CTCTTC 6 cut(s) 251, 533, 684, 1008, 1092, 1455
BstACI GRCGYC 1 cut(s) 578
BstAPI GCANNNNNTGC 5 cut(s) 671, 1067, 1196, 1274, 1592
BstAUI TGTACA 1 cut(s) 652
BstC8I GCNNGC 4 cut(s) 340, 909, 1008, 1767
BstDEI CTNAG 5 cut(s) 450, 483, 897, 1538, 1627
BstF5I GGATG 8 cut(s) 280, 379, 439, 775, 988, 1211, 1438, 1595
BstFNI CGCG 1 cut(s) 367
BstH2I RGCGCY 3 cut(s) 165, 186, 581
BstHHI GCGC 3 cut(s) 164, 185, 580
BstKTI GATC 3 cut(s) 298, 607, 1283
BstMAI GTCTC 2 cut(s) 36, 429
BstMBI GATC 3 cut(s) 295, 604, 1280
BstNI CCWGG 3 cut(s) 117, 994, 1600
BstNSI RCATGY 1 cut(s) 308
BstSCI CCNGG 5 cut(s) 115, 142, 221, 992, 1598
BstSFI CTRYAG 1 cut(s) 20
BstUI CGCG 1 cut(s) 367
BstX2I RGATCY 1 cut(s) 1280
BstXI CCANNNNNNTGG 2 cut(s) 116, 289
BstYI RGATCY 1 cut(s) 1280
BsuRI GGCC 2 cut(s) 64, 992
BtgZI GCGATG 2 cut(s) 135, 420
BtrI CACGTC 1 cut(s) 535
BtsCI GGATG 8 cut(s) 280, 379, 439, 775, 988, 1211, 1438, 1595
BtsIMutI CAGTG 1 cut(s) 1533
Cac8I GCNNGC 4 cut(s) 340, 909, 1008, 1767
CaiI CAGNNNCTG 1 cut(s) 1067
CfoI GCGC 3 cut(s) 164, 185, 580
Cfr13I GGNCC 2 cut(s) 1438, 1621
CseI GACGC 1 cut(s) 18
Csp6I GTAC 3 cut(s) 653, 841, 1666
CviAII CATG 3 cut(s) 305, 323, 926
CviQI GTAC 3 cut(s) 653, 841, 1666
DdeI CTNAG 5 cut(s) 450, 483, 897, 1538, 1627
DinI GGCGCC 1 cut(s) 579
DpnI GATC 3 cut(s) 297, 606, 1282
DpnII GATC 3 cut(s) 295, 604, 1280
EaeI YGGCCR 1 cut(s) 990
Eam1104I CTCTTC 6 cut(s) 251, 533, 684, 1008, 1092, 1455
EarI CTCTTC 6 cut(s) 251, 533, 684, 1008, 1092, 1455
EciI GGCGGA 2 cut(s) 73, 123
Eco130I CCWWGG 1 cut(s) 1581
Eco47I GGWCC 2 cut(s) 1438, 1621
Eco47III AGCGCT 1 cut(s) 184
Eco57I CTGAAG 5 cut(s) 540, 746, 1305, 1476, 1698
EcoRI GAATTC 1 cut(s) 77
EcoRII CCWGG 3 cut(s) 115, 992, 1598
EcoT14I CCWWGG 1 cut(s) 1581
EgeI GGCGCC 1 cut(s) 579
EheI GGCGCC 1 cut(s) 579
ErhI CCWWGG 1 cut(s) 1581
Esp3I CGTCTC 1 cut(s) 36
FaeI CATG 3 cut(s) 308, 326, 929
FalI AAGNNNNNCTT 4 cut(s) 1077, 1109, 1227, 1259
FaqI GGGAC 3 cut(s) 1194, 1537, 1646
FatI CATG 3 cut(s) 304, 322, 925
FauI CCCGC 2 cut(s) 426, 1171
FauNDI CATATG 1 cut(s) 1816
FblI GTMKAC 1 cut(s) 531
FokI GGATG 8 cut(s) 287, 386, 446, 782, 995, 1198, 1445, 1582
FspBI CTAG 7 cut(s) 230, 558, 597, 1028, 1340, 1673, 1770
GlaI GCGC 3 cut(s) 163, 184, 579
GsaI CCCAGC 1 cut(s) 201
HaeII RGCGCY 3 cut(s) 165, 186, 581
HaeIII GGCC 2 cut(s) 64, 992
HapII CCGG 3 cut(s) 143, 222, 644
HgaI GACGC 1 cut(s) 18
HhaI GCGC 3 cut(s) 164, 185, 580
Hin1I GRCGYC 1 cut(s) 578
Hin1II CATG 3 cut(s) 308, 326, 929
Hin6I GCGC 3 cut(s) 162, 183, 578
HinP1I GCGC 3 cut(s) 162, 183, 578
HindIII AAGCTT 1 cut(s) 1091
HinfI GANTC 6 cut(s) 55, 263, 1300, 1421, 1498, 1643
HpaII CCGG 3 cut(s) 143, 222, 644
HphI GGTGA 2 cut(s) 1621, 1771
Hpy166II GTNNAC 2 cut(s) 532, 1621
Hpy188I TCNGA 8 cut(s) 60, 179, 373, 520, 726, 1285, 1299, 1503
Hpy188III TCNNGA 5 cut(s) 230, 812, 869, 1085, 1454
Hpy8I GTNNAC 2 cut(s) 532, 1621
Hpy99I CGWCG 1 cut(s) 39
HpyCH4III ACNGT 1 cut(s) 1528
HpyCH4IV ACGT 1 cut(s) 534
HpyF3I CTNAG 5 cut(s) 450, 483, 897, 1538, 1627
HpySE526I ACGT 1 cut(s) 534
Hsp92I GRCGYC 1 cut(s) 578
Hsp92II CATG 3 cut(s) 308, 326, 929
HspAI GCGC 3 cut(s) 162, 183, 578
KasI GGCGCC 1 cut(s) 577
Kzo9I GATC 3 cut(s) 295, 604, 1280
LguI GCTCTTC 1 cut(s) 684
LmnI GCTCC 1 cut(s) 1328
MaeI CTAG 7 cut(s) 230, 558, 597, 1028, 1340, 1673, 1770
MaeII ACGT 1 cut(s) 534
MaeIII GTNAC 3 cut(s) 455, 1077, 1834
MalI GATC 3 cut(s) 297, 606, 1282
MboI GATC 3 cut(s) 295, 604, 1280
MfeI CAATTG 3 cut(s) 1194, 1368, 1493
MflI RGATCY 1 cut(s) 1280
MhlI GDGCHC 2 cut(s) 1125, 1333
MluI ACGCGT 1 cut(s) 365
Mly113I GGCGCC 1 cut(s) 578
MlyI GAGTC 2 cut(s) 1294, 1652
MseI TTAA 5 cut(s) 270, 953, 1040, 1151, 1544
MslI CAYNNNNRTG 2 cut(s) 303, 1251
MspA1I CMGCKG 1 cut(s) 1064
MspI CCGG 3 cut(s) 143, 222, 644
MspR9I CCNGG 5 cut(s) 117, 144, 223, 994, 1600
MunI CAATTG 3 cut(s) 1194, 1368, 1493
Mva1269I GAATGC 2 cut(s) 487, 1268
MvaI CCWGG 3 cut(s) 117, 994, 1600
MvnI CGCG 1 cut(s) 367
NarI GGCGCC 1 cut(s) 578
NciI CCSGG 2 cut(s) 144, 223
NdeI CATATG 1 cut(s) 1816
NdeII GATC 3 cut(s) 295, 604, 1280
NlaIII CATG 3 cut(s) 308, 326, 929
NlaIV GGNNCC 5 cut(s) 157, 247, 579, 1535, 1729
NmeAIII GCCGAG 1 cut(s) 373
NmuCI GTSAC 2 cut(s) 455, 1077
NspI RCATGY 1 cut(s) 308
PciI ACATGT 1 cut(s) 304
PciSI GCTCTTC 1 cut(s) 684
PcsI WCGNNNNNNNCGW 1 cut(s) 819
PctI GAATGC 2 cut(s) 487, 1268
PfeI GAWTC 4 cut(s) 55, 263, 1421, 1498
PflFI GACNNNGTC 1 cut(s) 734
PflMI CCANNNNNTGG 2 cut(s) 242, 1738
PleI GAGTC 2 cut(s) 1294, 1651
PluTI GGCGCC 1 cut(s) 581
PpsI GAGTC 2 cut(s) 1294, 1651
PscI ACATGT 1 cut(s) 304
Psp6I CCWGG 3 cut(s) 115, 992, 1598
PspFI CCCAGC 1 cut(s) 197
PspGI CCWGG 3 cut(s) 115, 992, 1598
PspN4I GGNNCC 5 cut(s) 157, 247, 579, 1535, 1729
PspPI GGNCC 2 cut(s) 1438, 1621
PstI CTGCAG 1 cut(s) 24
PstNI CAGNNNCTG 1 cut(s) 1067
PsuI RGATCY 1 cut(s) 1280
PsyI GACNNNGTC 1 cut(s) 734
PvuII CAGCTG 1 cut(s) 1064
RsaI GTAC 3 cut(s) 654, 842, 1667
RsaNI GTAC 3 cut(s) 653, 841, 1666
RseI CAYNNNNRTG 2 cut(s) 303, 1251
SapI GCTCTTC 1 cut(s) 684
SaqAI TTAA 5 cut(s) 270, 953, 1040, 1151, 1544
Sau3AI GATC 3 cut(s) 295, 604, 1280
Sau96I GGNCC 2 cut(s) 1438, 1621
ScaI AGTACT 1 cut(s) 1667
SchI GAGTC 2 cut(s) 1294, 1652
ScrFI CCNGG 5 cut(s) 117, 144, 223, 994, 1600
SduI GDGCHC 2 cut(s) 1125, 1333
SfcI CTRYAG 1 cut(s) 20
SfoI GGCGCC 1 cut(s) 579
SinI GGWCC 2 cut(s) 1438, 1621
SmiMI CAYNNNNRTG 2 cut(s) 303, 1251
SmlI CTYRAG 4 cut(s) 426, 710, 1073, 1118
SmoI CTYRAG 4 cut(s) 426, 710, 1073, 1118
SpeI ACTAGT 2 cut(s) 557, 596
SspDI GGCGCC 1 cut(s) 577
SspMI CTAG 7 cut(s) 230, 558, 597, 1028, 1340, 1673, 1770
StyD4I CCNGG 5 cut(s) 115, 142, 221, 992, 1598
StyI CCWWGG 1 cut(s) 1581
TaaI ACNGT 1 cut(s) 1528
TaiI ACGT 1 cut(s) 537
TaqI TCGA 5 cut(s) 360, 738, 813, 822, 870
TatI WGTACW 2 cut(s) 652, 1665
TauI GCSGC 5 cut(s) 89, 139, 142, 992, 1534
TfiI GAWTC 4 cut(s) 55, 263, 1421, 1498
Tru1I TTAA 5 cut(s) 270, 953, 1040, 1151, 1544
Tru9I TTAA 5 cut(s) 270, 953, 1040, 1151, 1544
TscAI CASTG 1 cut(s) 1533
TseFI GTSAC 2 cut(s) 455, 1077
Tsp45I GTSAC 2 cut(s) 455, 1077
TspDTI ATGAA 8 cut(s) 342, 702, 756, 813, 878, 981, 1160, 1235
TspGWI ACGGA 1 cut(s) 1650
TspRI CASTG 1 cut(s) 1533
Tth111I GACNNNGTC 1 cut(s) 734
Van91I CCANNNNNTGG 2 cut(s) 242, 1738
VpaK11BI GGWCC 2 cut(s) 1438, 1621
XapI RAATTY 3 cut(s) 77, 168, 1316
XbaI TCTAGA 1 cut(s) 229
XceI RCATGY 1 cut(s) 308
XmiI GTMKAC 1 cut(s) 531
XspI CTAG 7 cut(s) 230, 558, 597, 1028, 1340, 1673, 1770
ZrmI AGTACT 1 cut(s) 1667
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.