MD15G1329400.v1.1

Belongs to the MICOS complex subunit Mic60 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0011414
Physical Location & Seq
Reverse (-)
36282949 .. 36288748
5800 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1329400.v1.1.491

Sequence Viewer

Length: 1200 bp
ATGAGTAAAAACAATGGTCAATTGGACATTAACATCGAAGAGAGCTTATTTGAAAGGGTTTTAAATGAAGTTGCGTTTGGCGAAACTTTGTTTTTACAGGTTGCATTAGGTATTGGTGAAGAGCCATCAGGTTCTCTCCTCAAGGCATATCATCTGAAGGATGGGGCCAACGAAAGCATCGAATTTACTAATAGGCAGGATGGTAATGAATACGATAGCTTTCCTAATGAAAAAGAGGCGCTCAATGTAATCAAAGGGCTAAACGATGCTTACATATCCAAAGATGAAAAGTTAGTTCTTGACTTCTTACAAGCCATTCATACCGCTGAGAAAAGGCAAGCGGAGTTAGATGCTAATGTTTTTTCAGAAGAAAAGAGAAGGTTAAAGGAGAAGTATGAAAAGGAACTAAAGGATGCTGGGACTAGGGAGCTGATGCTTGCAGAAGAGGCAGCAATGCTAGACAAGGAATTAAAGAGAGAAGGGACAAAAGCAGCTGCTGCACTCAAGTCACTTCAAGAAAAGCTTGAAGAGATATATAAGACAGAACTTGAGCACAAGGAAAGTGAAGCAGAGATGAAGTTAAAAAAGGTTGAGGAATTAGCAAAAGCGGGATTGGCAGCAGCAATTGCAAGAGAGAAGACATCCCAAATTGAGAAAATGGCCGAAGCAAATCTTCATATAAATGCCTTGTGTGTGGCATTCTGTGCAAGGTCTGATGAAGCTCGTCAGACTCACTCTGCTCACAAGTTTGCCTTGGGAGCACTCGCACTAGATGATGCACTTTCAAAAGGATTACCAATCGAGAGAGAAGTAGAGGCTCTGCAAACTTATCTTGAAGGTATTGATAAAGATTCAATTTTGGATGTTTTTGATGCCTTGAAAGGGACAGTACGGCACCTCTGCTTAATTCCTCTTGGTGGTGGTGGCATTGTGGCGCATTCCTTGGCACATATTGCATCCTGGTTGAAGGTGAAGGAAGATGACCACTCTGGGGACGGAATTGAATCCATCATCAAGAAGGTGGAGTACTACCTTGCTGAAGGAAAGATAGCCGAAGCAGCAGAGGCATTTGAAGGAGTGAAAGGCACCCAAGCAACGGAAGTAGTGGGTGAATGGGTGAAGCGTGCTAGGAATAGGGCTATCACAGACCAAGCTCTAACACTTCTCCAATCTTATGCTACTTCCATCAGCGTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mitofilin

Protein Analysis

400

Amino Acids

44.09

Weight (kDa)

5.06

Isoelectric Point (pI)

41.89

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mitofilin PF09731 79 - 285 1.3e-19 Mitochondrial inner membrane protein
Mitofilin PF09731 288 - 394 2.1e-26 Mitochondrial inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0011414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39690
fragaria_vesca FvH4_1g21670 FvH4_1g21670 FvH4_1g21670
malus_domestica MD01G1023900.v1.1 MD15G1329400.v1.1
prunus_persica Prupe.6G178900_v2.0.a1
pyrus_communis pycom01g05150
rosa_chinensis RchiOBHm_Chr2g0115541
rosa_laevigata RLG00000018192
rosa_multiflora Rmu_ssc0000197.1_g000091
rosa_roxburghii Rroxscaffold_2G00128120 Rroxscaffold_2G00128990
rosa_rugosa Rorug02G0197700 Rorug02G0197800
rosa_samantha Rh2AG255100 Rh2BG266000 Rh2CG260300 Rh2DG263300
rosa_wichuraiana Rw2G019970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 894, 1085
AciI CCGC 3 cut(s) 324, 341, 608
AcoI YGGCCR 1 cut(s) 660
AcsI RAATTY 1 cut(s) 182
AcuI CTGAAG 2 cut(s) 176, 1059
AfaI GTAC 2 cut(s) 891, 1028
AfiI CCNNNNNNNGG 4 cut(s) 882, 917, 991, 1096
AjnI CCWGG 1 cut(s) 959
AjuI GAANNNNNNNTTGG 2 cut(s) 60, 92
AluBI AGCT 7 cut(s) 45, 219, 430, 494, 523, 722, 1154
AluI AGCT 7 cut(s) 45, 219, 430, 494, 523, 722, 1154
Alw21I GWGCWC 2 cut(s) 555, 763
AlwNI CAGNNNCTG 1 cut(s) 497
AoxI GGCC 2 cut(s) 165, 660
ApeKI GCWGC 7 cut(s) 449, 491, 494, 497, 617, 620, 1058
ApoI RAATTY 1 cut(s) 182
AspLEI GCGC 2 cut(s) 241, 937
AspS9I GGNCC 1 cut(s) 165
AsuHPI GGTGA 4 cut(s) 128, 982, 1121, 1129
BanI GGYRCC 2 cut(s) 894, 1085
BarI GAAGNNNNNNTAC 2 cut(s) 1010, 1042
BbsI GAAGAC 1 cut(s) 644
Bbv12I GWGCWC 2 cut(s) 555, 763
BbvI GCAGC 7 cut(s) 461, 481, 484, 503, 629, 632, 1070
BccI CCATC 5 cut(s) 133, 155, 194, 1016, 1193
BceAI ACGGC 1 cut(s) 908
BciT130I CCWGG 1 cut(s) 961
BfaI CTAG 4 cut(s) 423, 458, 770, 1128
BfoI RGCGCY 1 cut(s) 242
BisI GCNGC 7 cut(s) 450, 492, 495, 498, 618, 621, 1059
BlsI GCNGC 7 cut(s) 451, 493, 496, 499, 619, 622, 1060
BmcAI AGTACT 1 cut(s) 1028
Bme1390I CCNGG 1 cut(s) 961
BmgT120I GGNCC 1 cut(s) 165
BmiI GGNNCC 3 cut(s) 166, 896, 1087
BmrFI CCNGG 1 cut(s) 961
BmsI GCATC 8 cut(s) 186, 256, 340, 403, 423, 766, 862, 965
BpiI GAAGAC 1 cut(s) 644
BpuEI CTTGAG 3 cut(s) 125, 488, 569
BsaJI CCNNGG 2 cut(s) 753, 942
Bsc4I CCNNNNNNNGG 4 cut(s) 882, 917, 991, 1096
Bse3DI GCAATG 1 cut(s) 459
BseBI CCWGG 1 cut(s) 961
BseDI CCNNGG 2 cut(s) 753, 942
BseGI GGATG 6 cut(s) 166, 205, 418, 641, 868, 956
BseLI CCNNNNNNNGG 4 cut(s) 882, 917, 991, 1096
BseMI GCAATG 1 cut(s) 459
BseMII CTCAG 1 cut(s) 318
BseRI GAGGAG 1 cut(s) 128
BseXI GCAGC 7 cut(s) 461, 481, 484, 503, 629, 632, 1070
BseYI CCCAGC 1 cut(s) 416
BsgI GTGCAG 1 cut(s) 483
BshFI GGCC 2 cut(s) 167, 662
BshNI GGYRCC 2 cut(s) 894, 1085
BsiHKAI GWGCWC 2 cut(s) 555, 763
BslFI GGGAC 4 cut(s) 433, 496, 898, 1007
BslI CCNNNNNNNGG 4 cut(s) 882, 917, 991, 1096
BsmFI GGGAC 4 cut(s) 433, 496, 898, 1007
BsmI GAATGC 2 cut(s) 698, 937
BsnI GGCC 2 cut(s) 167, 662
Bsp1286I GDGCHC 2 cut(s) 555, 763
BspACI CCGC 3 cut(s) 324, 341, 608
BspANI GGCC 2 cut(s) 167, 662
BspCNI CTCAG 1 cut(s) 319
BspLI GGNNCC 3 cut(s) 166, 896, 1087
BspQI GCTCTTC 1 cut(s) 114
BspT107I GGYRCC 2 cut(s) 894, 1085
BsrDI GCAATG 1 cut(s) 459
BssECI CCNNGG 2 cut(s) 753, 942
BssT1I CCWWGG 2 cut(s) 753, 942
Bst2UI CCWGG 1 cut(s) 961
Bst4CI ACNGT 1 cut(s) 889
Bst6I CTCTTC 4 cut(s) 33, 114, 438, 522
BstAPI GCANNNNNTGC 4 cut(s) 497, 626, 704, 953
BstC8I GCNNGC 3 cut(s) 339, 438, 1125
BstDEI CTNAG 1 cut(s) 327
BstF5I GGATG 6 cut(s) 166, 205, 418, 641, 868, 956
BstH2I RGCGCY 1 cut(s) 242
BstHHI GCGC 2 cut(s) 241, 937
BstMWI GCNNNNNNNGC 9 cut(s) 446, 497, 614, 626, 704, 758, 953, 1058, 1064
BstNI CCWGG 1 cut(s) 961
BstSCI CCNGG 1 cut(s) 959
BstV1I GCAGC 7 cut(s) 461, 481, 484, 503, 629, 632, 1070
BstV2I GAAGAC 1 cut(s) 644
BsuRI GGCC 2 cut(s) 167, 662
BtsCI GGATG 6 cut(s) 166, 205, 418, 641, 868, 956
Cac8I GCNNGC 3 cut(s) 339, 438, 1125
CaiI CAGNNNCTG 1 cut(s) 497
CfoI GCGC 2 cut(s) 241, 937
Cfr13I GGNCC 1 cut(s) 165
Csp6I GTAC 2 cut(s) 890, 1027
CviQI GTAC 2 cut(s) 890, 1027
DdeI CTNAG 1 cut(s) 327
DraI TTTAAA 1 cut(s) 63
EaeI YGGCCR 1 cut(s) 660
Eam1104I CTCTTC 4 cut(s) 33, 114, 438, 522
EarI CTCTTC 4 cut(s) 33, 114, 438, 522
Eco130I CCWWGG 2 cut(s) 753, 942
Eco57I CTGAAG 2 cut(s) 176, 1059
EcoRII CCWGG 1 cut(s) 959
EcoT14I CCWWGG 2 cut(s) 753, 942
ErhI CCWWGG 2 cut(s) 753, 942
FalI AAGNNNNNCTT 6 cut(s) 507, 539, 657, 689, 737, 769
FaqI GGGAC 4 cut(s) 433, 496, 898, 1007
FauI CCCGC 1 cut(s) 601
Fnu4HI GCNGC 7 cut(s) 450, 492, 495, 498, 618, 621, 1059
FokI GGATG 6 cut(s) 173, 212, 425, 628, 875, 943
Fsp4HI GCNGC 7 cut(s) 450, 492, 495, 498, 618, 621, 1059
FspBI CTAG 4 cut(s) 423, 458, 770, 1128
GlaI GCGC 2 cut(s) 240, 936
GluI GCNGC 7 cut(s) 450, 492, 495, 498, 618, 621, 1059
GsaI CCCAGC 1 cut(s) 420
HaeII RGCGCY 1 cut(s) 242
HaeIII GGCC 2 cut(s) 167, 662
HhaI GCGC 2 cut(s) 241, 937
Hin6I GCGC 2 cut(s) 239, 935
HinP1I GCGC 2 cut(s) 239, 935
HindIII AAGCTT 1 cut(s) 521
HinfI GANTC 3 cut(s) 730, 851, 1004
HphI GGTGA 4 cut(s) 128, 982, 1121, 1129
Hpy188I TCNGA 4 cut(s) 156, 367, 715, 729
Hpy188III TCNNGA 5 cut(s) 299, 515, 802, 833, 1015
HpyAV CCTTC 9 cut(s) 151, 372, 473, 830, 961, 967, 1012, 1034, 1067
HpyCH4III ACNGT 1 cut(s) 889
HpyCH4V TGCA 8 cut(s) 104, 440, 500, 629, 707, 779, 823, 956
HpyF10VI GCNNNNNNNGC 9 cut(s) 446, 497, 614, 626, 704, 758, 953, 1058, 1064
HpyF3I CTNAG 1 cut(s) 327
HspAI GCGC 2 cut(s) 239, 935
LguI GCTCTTC 1 cut(s) 114
LmnI GCTCC 2 cut(s) 427, 758
LpnPI CCDG 7 cut(s) 83, 114, 182, 402, 946, 973, 975
Lsp1109I GCAGC 7 cut(s) 461, 481, 484, 503, 629, 632, 1070
LweI GCATC 8 cut(s) 186, 256, 340, 403, 423, 766, 862, 965
MaeI CTAG 4 cut(s) 423, 458, 770, 1128
MaeIII GTNAC 1 cut(s) 507
MboII GAAGA 8 cut(s) 50, 131, 380, 455, 539, 649, 665, 989
MfeI CAATTG 2 cut(s) 20, 624
MhlI GDGCHC 2 cut(s) 555, 763
MluCI AATT 9 cut(s) 20, 182, 467, 596, 624, 648, 855, 906, 999
MlyI GAGTC 1 cut(s) 724
MnlI CCTC 8 cut(s) 149, 229, 439, 586, 808, 908, 921, 1057
MseI TTAA 6 cut(s) 30, 62, 383, 470, 581, 905
MslI CAYNNNNRTG 1 cut(s) 681
MspA1I CMGCKG 2 cut(s) 326, 494
MspR9I CCNGG 1 cut(s) 961
MunI CAATTG 2 cut(s) 20, 624
Mva1269I GAATGC 2 cut(s) 698, 937
MvaI CCWGG 1 cut(s) 961
MwoI GCNNNNNNNGC 9 cut(s) 446, 497, 614, 626, 704, 758, 953, 1058, 1064
NlaIV GGNNCC 3 cut(s) 166, 896, 1087
NmuCI GTSAC 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 114
PcsI WCGNNNNNNNCGW 1 cut(s) 177
PctI GAATGC 2 cut(s) 698, 937
PfeI GAWTC 2 cut(s) 851, 1004
PkrI GCNGC 7 cut(s) 451, 493, 496, 499, 619, 622, 1060
PleI GAGTC 1 cut(s) 724
PpsI GAGTC 1 cut(s) 724
Psp6I CCWGG 1 cut(s) 959
PspFI CCCAGC 1 cut(s) 416
PspGI CCWGG 1 cut(s) 959
PspN4I GGNNCC 3 cut(s) 166, 896, 1087
PspPI GGNCC 1 cut(s) 165
PstNI CAGNNNCTG 1 cut(s) 497
PvuII CAGCTG 1 cut(s) 494
RsaI GTAC 2 cut(s) 891, 1028
RsaNI GTAC 2 cut(s) 890, 1027
RseI CAYNNNNRTG 1 cut(s) 681
SapI GCTCTTC 1 cut(s) 114
SaqAI TTAA 6 cut(s) 30, 62, 383, 470, 581, 905
SatI GCNGC 7 cut(s) 450, 492, 495, 498, 618, 621, 1059
Sau96I GGNCC 1 cut(s) 165
ScaI AGTACT 1 cut(s) 1028
SchI GAGTC 1 cut(s) 724
ScrFI CCNGG 1 cut(s) 961
SduI GDGCHC 2 cut(s) 555, 763
SfaNI GCATC 8 cut(s) 186, 256, 340, 403, 423, 766, 862, 965
SmiMI CAYNNNNRTG 1 cut(s) 681
SmlI CTYRAG 3 cut(s) 140, 503, 548
SmoI CTYRAG 3 cut(s) 140, 503, 548
Sse9I AATT 9 cut(s) 20, 182, 467, 596, 624, 648, 855, 906, 999
SsiI CCGC 3 cut(s) 324, 341, 608
SspMI CTAG 4 cut(s) 423, 458, 770, 1128
StyD4I CCNGG 1 cut(s) 959
StyI CCWWGG 2 cut(s) 753, 942
TaaI ACNGT 1 cut(s) 889
TaqI TCGA 3 cut(s) 36, 180, 801
TasI AATT 9 cut(s) 20, 182, 467, 596, 624, 648, 855, 906, 999
TatI WGTACW 1 cut(s) 1026
TfiI GAWTC 2 cut(s) 851, 1004
Tru1I TTAA 6 cut(s) 30, 62, 383, 470, 581, 905
Tru9I TTAA 6 cut(s) 30, 62, 383, 470, 581, 905
TseFI GTSAC 1 cut(s) 507
TseI GCWGC 7 cut(s) 449, 491, 494, 497, 617, 620, 1058
Tsp45I GTSAC 1 cut(s) 507
TspDTI ATGAA 9 cut(s) 81, 222, 243, 300, 308, 411, 590, 665, 732
TspGWI ACGGA 2 cut(s) 1011, 1112
XapI RAATTY 1 cut(s) 182
XspI CTAG 4 cut(s) 423, 458, 770, 1128
ZrmI AGTACT 1 cut(s) 1028
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.