Rh2DG263300

Belongs to the MICOS complex subunit Mic60 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2D
OG0011414
Physical Location & Seq
Reverse (-)
28395648 .. 28401355
5708 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2DG263300.1

Sequence Viewer

Length: 1596 bp
ATGTTGCGAAGGTCCATTCTCCAAATTTCGTCGCGTCGGGCCGTTCGGAGGATCCAAAGTCAGCACGTTCCTAGGTTTTTGAGAAAGGAGTTCTCCGCCGCGGTCCAGAAGAATCCGGCGGCGGCTCAGCCCGGCGGTCCGGGCAAGCCACCGGAGAAGTCTGGCAGTTCAAACGCTTTTATATTTGGAAGTCTTGTTGTTGGTGGAGGGGCTGCTTTGGCTTACGGAGCTGGCTATCTGGACCCGATTCTCGGCCGGAAGAAGCAGCCGGAACCGGAAGTTGAAGTGATTGTCAATGAGGATGTAGGAGTGGAAGAGAATGTGCCGCGTGTGGAGGAAGAGAGTAGCAAACTGAGCTTTGCTCCTGAGGTTTCTGAGGAGAAGAGTGATGCACATTCGGAGGATGTTGCCAAGTTTGAAGGTTTGGGTGGAAGTGAAGGCGAAAGTGAGTCGAAGGTGGAAGAGAAATCTGTGGTCAGTGATGTAACAGATGAGAAGGATTTGGGGGAGGTTTCGCAAAATGCAAATGTTGGAGTATCGTCTGAAGAGAATGTAGATGATAAAAGTGTAGAGGAGAAAACTAGTGAGGTGCCCAAGGAGGAGGTTGAAACTGCACCGGTGTTTACCGAGACTGATCAGCAAGAGAATGAGATTAAAGCATTGGCTCCTCAACGGGAAGTCTTCTATGACAAGGCACAGGATGCAATAGCTAATGTTGAAGAACCACCGAGTTCTTTGCTCAGTGAATATAATCTGAAGGATGAGGCTGATGGAGGCAGTGAGACTAATATTAGTACACAAGGCAGTAATGAATACAATCCACTTCCTAAAGAAAAAGAGGCCTTAATTGGTGCTATTGAAGGGTTGGATGATGCTTACATATCCAAAGATGGAAAGTTAGTTCTTGACTTCTTGCAAGCAATTCATGCTGCTGAAAAAAGGCAAGCTGAGTTAGATGCTCGTGTTTCTTCTGAAGAAAAGAGAGCATTAAAGGAGAAGTATGAAAAGAAACTGAAGGATGCTGGCATTAGGGAAATGATGCTTGCAGAAGAGGCAGCAATGCTAGACAAGGAATTAAAGAGAGAAAGAGCAAAAGCAGTTGCTGCACTCAAGTCACTTCAAGAAAAACTTGAAGAGAAATATAAGACAGAACTTGAGCAGAAGGAAAACGAAGCAGAAATGAAGTTAAAAAAGGTTGAGGAATTAGCAAAAGCTGAACTGGCTGCCACAATTGCAAGTGAGAAGGCATCCCAGATTGAGAAAATGACAGAAGCAAATCTTCATATAAATGCCTTGTGTGTTGCATTCTATGCAAGATCTGAAGAAGCTCGTCAGACTCACTCTGCTCACAAGCTTGCTTTGGGGGCACTTGCACTGGAAGATGCACTTTCCAAAGGGTTACCAATCCAGACAGAAATAGAGGCTTTGGACACTTATCTTGAAGGCATTGATAAAAATTCAATTTTGGATTTGGTCCTATCATCACTTCCTGAGGAAACACGGAGGAGTGGCACAGATACCTTGTTGCAATTGAATCAGAAGGCTAGTTATCTTCTTACTTCTTCCTTAGATGTTTGCTGGTTGTTACTCTTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mitofilin

Protein Analysis

531

Amino Acids

58.21

Weight (kDa)

4.75

Isoelectric Point (pI)

57.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mitofilin PF09731 104 - 514 9.2e-29 Mitochondrial inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39690
fragaria_vesca FvH4_1g21670 FvH4_1g21670 FvH4_1g21670
malus_domestica MD01G1023900.v1.1 MD15G1329400.v1.1
prunus_persica Prupe.6G178900_v2.0.a1
pyrus_communis pycom01g05150
rosa_chinensis RchiOBHm_Chr2g0115541
rosa_laevigata RLG00000018192
rosa_multiflora Rmu_ssc0000197.1_g000091
rosa_roxburghii Rroxscaffold_2G00128120 Rroxscaffold_2G00128990
rosa_rugosa Rorug02G0197700 Rorug02G0197800
rosa_samantha Rh2AG255100 Rh2BG266000 Rh2CG260300 Rh2DG263300
rosa_wichuraiana Rw2G019970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1594
AccB1I GGYRCC 1 cut(s) 589
AccII CGCG 3 cut(s) 34, 101, 328
AciI CCGC 7 cut(s) 96, 99, 101, 119, 122, 135, 326
AclWI GGATC 2 cut(s) 46, 59
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 2 cut(s) 24, 1456
AcuI CTGAAG 5 cut(s) 564, 776, 993, 1034, 1341
AfaI GTAC 1 cut(s) 796
AfiI CCNNNNNNNGG 2 cut(s) 48, 251
AgeI ACCGGT 1 cut(s) 616
AhlI ACTAGT 1 cut(s) 581
AloI GAACNNNNNNTCC 2 cut(s) 885, 917
AluBI AGCT 7 cut(s) 230, 357, 710, 947, 1214, 1328, 1354
AluI AGCT 7 cut(s) 230, 357, 710, 947, 1214, 1328, 1354
Alw26I GTCTC 2 cut(s) 623, 776
AlwI GGATC 2 cut(s) 46, 59
AlwNI CAGNNNCTG 1 cut(s) 1103
AoxI GGCC 3 cut(s) 39, 253, 840
ApeKI GCWGC 6 cut(s) 212, 265, 929, 1055, 1103, 1223
ApoI RAATTY 2 cut(s) 24, 1456
AsiGI ACCGGT 1 cut(s) 616
AspA2I CCTAGG 1 cut(s) 71
AspS9I GGNCC 6 cut(s) 12, 39, 103, 137, 241, 1474
AsuC2I CCSGG 2 cut(s) 132, 141
AvaII GGWCC 5 cut(s) 12, 103, 137, 241, 1474
AvrII CCTAGG 1 cut(s) 71
AxyI CCTNAGG 2 cut(s) 366, 1491
BaeGI GKGCMC 2 cut(s) 594, 1369
BamHI GGATCC 1 cut(s) 51
BanI GGYRCC 1 cut(s) 589
BauI CACGAG 1 cut(s) 960
BbsI GAAGAC 1 cut(s) 673
BbvI GCAGC 6 cut(s) 199, 277, 916, 1067, 1090, 1210
BccI CCATC 2 cut(s) 764, 884
BceAI ACGGC 1 cut(s) 26
BcgI CGANNNNNNTGC 2 cut(s) 718, 752
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 2 cut(s) 132, 141
BcoDI GTCTC 2 cut(s) 623, 776
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 4 cut(s) 72, 582, 1064, 1545
BglII AGATCT 1 cut(s) 1316
BlnI CCTAGG 1 cut(s) 71
BlpI GCTNAGC 1 cut(s) 126
Bme1390I CCNGG 2 cut(s) 132, 141
Bme18I GGWCC 5 cut(s) 12, 103, 137, 241, 1474
BmgT120I GGNCC 6 cut(s) 12, 39, 103, 137, 241, 1474
BmiI GGNNCC 5 cut(s) 53, 243, 273, 591, 666
BmrFI CCNGG 2 cut(s) 132, 141
BmsI GCATC 8 cut(s) 379, 691, 862, 946, 1009, 1029, 1256, 1372
BpiI GAAGAC 1 cut(s) 673
BplI GAGNNNNNCTC 2 cut(s) 346, 378
Bpu1102I GCTNAGC 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 1094, 1175
BpuMI CCSGG 2 cut(s) 132, 141
BsaJI CCNNGG 3 cut(s) 71, 99, 594
BsaWI WCCGGW 3 cut(s) 151, 274, 616
Bsc4I CCNNNNNNNGG 2 cut(s) 48, 251
Bse118I RCCGGY 1 cut(s) 616
Bse1I ACTGG 2 cut(s) 1224, 1380
Bse21I CCTNAGG 2 cut(s) 366, 1491
Bse3DI GCAATG 1 cut(s) 1065
BseDI CCNNGG 3 cut(s) 71, 99, 594
BseGI GGATG 7 cut(s) 307, 409, 706, 766, 874, 1024, 1247
BseLI CCNNNNNNNGG 2 cut(s) 48, 251
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 7 cut(s) 140, 344, 357, 366, 754, 939, 1482
BseNI ACTGG 2 cut(s) 1224, 1380
BseRI GAGGAG 5 cut(s) 392, 587, 614, 657, 1519
BseSI GKGCMC 2 cut(s) 594, 1369
BseX3I CGGCCG 1 cut(s) 253
BseXI GCAGC 6 cut(s) 199, 277, 916, 1067, 1090, 1210
BsgI GTGCAG 2 cut(s) 597, 1089
Bsh1236I CGCG 3 cut(s) 34, 101, 328
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 3 cut(s) 41, 255, 842
BshNI GGYRCC 1 cut(s) 589
BshTI ACCGGT 1 cut(s) 616
BsiEI CGRYCG 1 cut(s) 256
BsiSI CCGG 8 cut(s) 116, 132, 140, 152, 256, 269, 275, 617
BslI CCNNNNNNNGG 2 cut(s) 48, 251
BsmAI GTCTC 2 cut(s) 623, 776
BsmI GAATGC 1 cut(s) 1304
BsnI GGCC 3 cut(s) 41, 255, 842
Bsp1286I GDGCHC 2 cut(s) 594, 1369
Bsp143I GATC 3 cut(s) 51, 634, 1316
Bsp1720I GCTNAGC 1 cut(s) 126
BspACI CCGC 7 cut(s) 96, 99, 101, 119, 122, 135, 326
BspANI GGCC 3 cut(s) 41, 255, 842
BspCNI CTCAG 7 cut(s) 139, 345, 358, 367, 753, 940, 1483
BspFNI CGCG 3 cut(s) 34, 101, 328
BspLI GGNNCC 5 cut(s) 53, 243, 273, 591, 666
BspPI GGATC 2 cut(s) 46, 59
BspT107I GGYRCC 1 cut(s) 589
BsrDI GCAATG 1 cut(s) 1065
BsrFI RCCGGY 1 cut(s) 616
BsrI ACTGG 2 cut(s) 1224, 1380
BssAI RCCGGY 1 cut(s) 616
BssECI CCNNGG 3 cut(s) 71, 99, 594
BssMI GATC 3 cut(s) 51, 634, 1316
BssSI CACGAG 1 cut(s) 960
BssT1I CCWWGG 2 cut(s) 71, 594
Bst2BI CACGAG 1 cut(s) 960
Bst6I CTCTTC 7 cut(s) 309, 333, 377, 456, 540, 1044, 1128
BstAPI GCANNNNNTGC 4 cut(s) 701, 926, 1103, 1310
BstC8I GCNNGC 7 cut(s) 146, 232, 918, 945, 1024, 1044, 1356
BstDEI CTNAG 8 cut(s) 126, 353, 366, 375, 740, 948, 1491, 1567
BstDSI CCRYGG 1 cut(s) 99
BstEII GGTNACC 1 cut(s) 1398
BstF5I GGATG 7 cut(s) 307, 409, 706, 766, 874, 1024, 1247
BstFNI CGCG 3 cut(s) 34, 101, 328
BstKTI GATC 3 cut(s) 54, 637, 1319
BstMAI GTCTC 2 cut(s) 623, 776
BstMBI GATC 3 cut(s) 51, 634, 1316
BstMCI CGRYCG 1 cut(s) 256
BstPI GGTNACC 1 cut(s) 1398
BstSCI CCNGG 2 cut(s) 130, 139
BstSLI GKGCMC 2 cut(s) 594, 1369
BstUI CGCG 3 cut(s) 34, 101, 328
BstV1I GCAGC 6 cut(s) 199, 277, 916, 1067, 1090, 1210
BstV2I GAAGAC 1 cut(s) 673
BstX2I RGATCY 2 cut(s) 51, 1316
BstYI RGATCY 2 cut(s) 51, 1316
BstZI CGGCCG 1 cut(s) 253
Bsu36I CCTNAGG 2 cut(s) 366, 1491
BsuRI GGCC 3 cut(s) 41, 255, 842
BtgI CCRYGG 1 cut(s) 99
BtsCI GGATG 7 cut(s) 307, 409, 706, 766, 874, 1024, 1247
BtsI GCAGTG 1 cut(s) 784
BtsIMutI CAGTG 4 cut(s) 484, 748, 784, 1373
Cac8I GCNNGC 7 cut(s) 146, 232, 918, 945, 1024, 1044, 1356
CaiI CAGNNNCTG 1 cut(s) 1103
Cfr10I RCCGGY 1 cut(s) 616
Cfr13I GGNCC 6 cut(s) 12, 39, 103, 137, 241, 1474
Cfr42I CCGCGG 1 cut(s) 102
CpoI CGGWCCG 1 cut(s) 137
CseI GACGC 1 cut(s) 23
Csp6I GTAC 1 cut(s) 795
CspAI ACCGGT 1 cut(s) 616
CspI CGGWCCG 1 cut(s) 137
CviAII CATG 1 cut(s) 926
CviQI GTAC 1 cut(s) 795
DdeI CTNAG 8 cut(s) 126, 353, 366, 375, 740, 948, 1491, 1567
DpnI GATC 3 cut(s) 53, 636, 1318
DpnII GATC 3 cut(s) 51, 634, 1316
EaeI YGGCCR 1 cut(s) 253
EagI CGGCCG 1 cut(s) 253
Eam1104I CTCTTC 7 cut(s) 309, 333, 377, 456, 540, 1044, 1128
EarI CTCTTC 7 cut(s) 309, 333, 377, 456, 540, 1044, 1128
EciI GGCGGA 1 cut(s) 85
EclXI CGGCCG 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 71, 594
Eco147I AGGCCT 1 cut(s) 842
Eco47I GGWCC 5 cut(s) 12, 103, 137, 241, 1474
Eco52I CGGCCG 1 cut(s) 253
Eco57I CTGAAG 5 cut(s) 564, 776, 993, 1034, 1341
Eco81I CCTNAGG 2 cut(s) 366, 1491
Eco91I GGTNACC 1 cut(s) 1398
EcoO65I GGTNACC 1 cut(s) 1398
EcoT14I CCWWGG 2 cut(s) 71, 594
ErhI CCWWGG 2 cut(s) 71, 594
FaeI CATG 1 cut(s) 929
FalI AAGNNNNNCTT 6 cut(s) 1113, 1145, 1263, 1295, 1371, 1403
FatI CATG 1 cut(s) 925
FbaI TGATCA 1 cut(s) 634
FokI GGATG 7 cut(s) 314, 416, 713, 773, 881, 1031, 1234
FspBI CTAG 4 cut(s) 72, 582, 1064, 1545
HaeIII GGCC 3 cut(s) 41, 255, 842
HapII CCGG 8 cut(s) 116, 132, 140, 152, 256, 269, 275, 617
HgaI GACGC 1 cut(s) 23
Hin1II CATG 1 cut(s) 929
HindIII AAGCTT 1 cut(s) 1352
HinfI GANTC 5 cut(s) 112, 247, 449, 1336, 1534
HpaII CCGG 8 cut(s) 116, 132, 140, 152, 256, 269, 275, 617
Hpy166II GTNNAC 2 cut(s) 624, 797
Hpy188I TCNGA 9 cut(s) 48, 376, 400, 544, 756, 973, 1321, 1335, 1539
Hpy188III TCNNGA 8 cut(s) 106, 239, 365, 905, 1121, 1408, 1439, 1490
Hpy8I GTNNAC 2 cut(s) 624, 797
Hpy99I CGWCG 2 cut(s) 34, 39
HpyCH4IV ACGT 1 cut(s) 66
HpyF3I CTNAG 8 cut(s) 126, 353, 366, 375, 740, 948, 1491, 1567
HpySE526I ACGT 1 cut(s) 66
Hsp92II CATG 1 cut(s) 929
Ksp22I TGATCA 1 cut(s) 634
KspI CCGCGG 1 cut(s) 102
Kzo9I GATC 3 cut(s) 51, 634, 1316
LmnI GCTCC 3 cut(s) 227, 367, 670
Lsp1109I GCAGC 6 cut(s) 199, 277, 916, 1067, 1090, 1210
LweI GCATC 8 cut(s) 379, 691, 862, 946, 1009, 1029, 1256, 1372
MaeI CTAG 4 cut(s) 72, 582, 1064, 1545
MaeII ACGT 1 cut(s) 66
MaeIII GTNAC 4 cut(s) 484, 1113, 1398, 1584
MalI GATC 3 cut(s) 53, 636, 1318
MboI GATC 3 cut(s) 51, 634, 1316
MfeI CAATTG 2 cut(s) 1230, 1529
MflI RGATCY 2 cut(s) 51, 1316
MhlI GDGCHC 2 cut(s) 594, 1369
MluCI AATT 9 cut(s) 24, 846, 921, 1073, 1202, 1230, 1456, 1461, 1529
MlyI GAGTC 2 cut(s) 458, 1330
MmeI TCCRAC 2 cut(s) 511, 846
MseI TTAA 5 cut(s) 654, 845, 989, 1076, 1187
MslI CAYNNNNRTG 1 cut(s) 1287
MspA1I CMGCKG 1 cut(s) 101
MspI CCGG 8 cut(s) 116, 132, 140, 152, 256, 269, 275, 617
MspR9I CCNGG 2 cut(s) 132, 141
MunI CAATTG 2 cut(s) 1230, 1529
Mva1269I GAATGC 1 cut(s) 1304
MvnI CGCG 3 cut(s) 34, 101, 328
NciI CCSGG 2 cut(s) 132, 141
NdeII GATC 3 cut(s) 51, 634, 1316
NlaIII CATG 1 cut(s) 929
NlaIV GGNNCC 5 cut(s) 53, 243, 273, 591, 666
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 1 cut(s) 1113
PceI AGGCCT 1 cut(s) 842
PctI GAATGC 1 cut(s) 1304
PfeI GAWTC 3 cut(s) 112, 247, 1534
PinAI ACCGGT 1 cut(s) 616
PleI GAGTC 2 cut(s) 457, 1330
PpsI GAGTC 2 cut(s) 457, 1330
PsiI TTATAA 1 cut(s) 1594
PspEI GGTNACC 1 cut(s) 1398
PspN4I GGNNCC 5 cut(s) 53, 243, 273, 591, 666
PspPI GGNCC 6 cut(s) 12, 39, 103, 137, 241, 1474
PstNI CAGNNNCTG 1 cut(s) 1103
PsuI RGATCY 2 cut(s) 51, 1316
RsaI GTAC 1 cut(s) 796
RsaNI GTAC 1 cut(s) 795
RseI CAYNNNNRTG 1 cut(s) 1287
Rsr2I CGGWCCG 1 cut(s) 137
RsrII CGGWCCG 1 cut(s) 137
SacII CCGCGG 1 cut(s) 102
SaqAI TTAA 5 cut(s) 654, 845, 989, 1076, 1187
Sau3AI GATC 3 cut(s) 51, 634, 1316
Sau96I GGNCC 6 cut(s) 12, 39, 103, 137, 241, 1474
SchI GAGTC 2 cut(s) 458, 1330
ScrFI CCNGG 2 cut(s) 132, 141
SduI GDGCHC 2 cut(s) 594, 1369
SfaNI GCATC 8 cut(s) 379, 691, 862, 946, 1009, 1029, 1256, 1372
Sfr303I CCGCGG 1 cut(s) 102
SgrAI CRCCGGYG 1 cut(s) 616
SgrBI CCGCGG 1 cut(s) 102
SinI GGWCC 5 cut(s) 12, 103, 137, 241, 1474
SmiMI CAYNNNNRTG 1 cut(s) 1287
SmlI CTYRAG 2 cut(s) 1109, 1154
SmoI CTYRAG 2 cut(s) 1109, 1154
SpeI ACTAGT 1 cut(s) 581
Sse9I AATT 9 cut(s) 24, 846, 921, 1073, 1202, 1230, 1456, 1461, 1529
SseBI AGGCCT 1 cut(s) 842
SsiI CCGC 7 cut(s) 96, 99, 101, 119, 122, 135, 326
SspI AATATT 1 cut(s) 790
SspMI CTAG 4 cut(s) 72, 582, 1064, 1545
StuI AGGCCT 1 cut(s) 842
StyD4I CCNGG 2 cut(s) 130, 139
StyI CCWWGG 2 cut(s) 71, 594
TaiI ACGT 1 cut(s) 69
TaqI TCGA 1 cut(s) 452
TasI AATT 9 cut(s) 24, 846, 921, 1073, 1202, 1230, 1456, 1461, 1529
TatI WGTACW 1 cut(s) 794
TauI GCSGC 4 cut(s) 101, 122, 125, 328
TfiI GAWTC 3 cut(s) 112, 247, 1534
Tru1I TTAA 5 cut(s) 654, 845, 989, 1076, 1187
Tru9I TTAA 5 cut(s) 654, 845, 989, 1076, 1187
TscAI CASTG 4 cut(s) 484, 748, 784, 1380
TseFI GTSAC 1 cut(s) 1113
TseI GCWGC 6 cut(s) 212, 265, 929, 1055, 1103, 1223
Tsp45I GTSAC 1 cut(s) 1113
TspDTI ATGAA 5 cut(s) 825, 914, 1017, 1196, 1271
TspGWI ACGGA 2 cut(s) 240, 1516
TspRI CASTG 4 cut(s) 484, 748, 784, 1380
VpaK11BI GGWCC 5 cut(s) 12, 103, 137, 241, 1474
XapI RAATTY 2 cut(s) 24, 1456
XmaJI CCTAGG 1 cut(s) 71
XspI CTAG 4 cut(s) 72, 582, 1064, 1545
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.