Rroxscaffold_2G00128990

Belongs to the MICOS complex subunit Mic60 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011414
Physical Location & Seq
Forward (+)
64762308 .. 64767637
5330 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00128990.1

Sequence Viewer

Length: 1857 bp
ATGTTGCGAAGGTCCATTCTCCAAATTTCGTCGCGTCGGGCCGTTCGGAGGATCCAAAGTCAGCACGTTCCTAGGTTTTTGAGAAAGGAGTTCTCCGCCGCAGTCCAGAAGAATCCGGCTGCGGCTCAGCCCGGCGGTCCGGGCAAGCCACCGGAGAAGTCTGGCGGTTCAAACGCTTTAATCTTTGGAGGTCTTGTTGTTGGCGGTGGGGCTGCTTTAGCTTACGGAGCTGGCTATCTGGACCCGATTCTCGGCCGGAAGAAGCAGCCGGAACCGGAAGTTGAAGTGATTGTCAATGAGGATGTAGGTGTGGAAGAGAATGTGCCGCGTGTGGAGGAAGAGAGTAGCAAACTGAACTTTGCTCCTGAGGTTTCTGAGGAGAAGAGTGATGCACATTCGGAGGATGTTGCCAAGTTTGAAGGTTTGGGTGGAAGTGAAGGCGAAAGCGAGTCGAAGTTGGAAGAGAAATCTGTGGTCAGTGACGTAACAGCTGAGAAGGATTTGGGGGAGGTTTCACAAAATGCAAATGTTGGAGTTTCGTCTGAAGAGAATGTAGATAATAAAAGTGTAGAGGAGAAAACTAGTGAGGTGCCCAAGGAGGAGGTTGAAACTGCACCGGTGTTTGCTGAGACTGATCAGCAAGAGAATGAGATTAAAGCATTGGCTCCTCAACAGGAAATCATAGAAGACAAGGCACAGGATGCAATAGCTAATGTTGAAGAACCACCAAGTTCTTTGCTCAGTGAATATAATCTGAAGGATGAGGCTGATGGAGGCAGTGAGACTATTATTAGTACACAAGGCAGTGATGAATACAATCCACTTCCTAAAGAAAAAGAGGCCTCAATTGGTGTTATTGAAGGGTTGGATGATGCTTACATATCCAAAGATGGAAAGTTAGTTCTTGACTTCTTGCAAGCAATTCATGCTGCTGAAAAAAGGCAAGCTGAGTTAGATGCTCGTGTTTCTTCTGAAGAAAAGAGAGCATTAAAGGAGAAGTATGAAAAGAAACTGAAGGATGCTGGCATTAGGGAAATGATGCTTGCAGAAGAGGCAGCAATGCTAGACAAGGAATTAAAGAGAGAAAGAGCAAAAGCAGTTGCTGCACTCAAGTCACTTCAAGAAAAACTTGAAGAGAAATATAAGACAGAACTTGAGCAGAAGGTGCACAACACTTTGATGCTTGATCCTGAAAACGAAGCAGAAATGAAGTTAAAAAAGGTTGAGGAATTAGCAAAAGCTGAACTGGCTGCCGCAATTGCAAGTGAGAAGGCATCCCAGATTGAGAAAATGACAGAAGCAAATCTTCATGGGGCACTTGCACTGGAAGATGCACTTTCCAAAGGGTTACCAATCCACACAGAAATAGAGGCTTTGGACACTAATCTTGAAGGCATTGATAAAAATTCAATTTTGGATTTGGTCCTGTCATCACTTCCTGAGGAAACACGGAGGAGTGGCACAGATACCTTGTTGCAATTGAATCAGAAGGCTAGTTATCTTCTTACTTCTTCCTTAGATTTTGATGCCTTAAAAGGGACAGTACGACACTTCAGCCTAATCCCTCCTGGTGGTGGGGGCATTTTGGCTCACTCTTTAGCACGGATAGCATCCTGGTTGAAGGTGAAGGAAGTAGATCAATCTGGAGATGGGATTGAGTCTATCATCAATAAAGTGGAGTGCCACCTCGCTGAAGGAAAATTAGTCGAAGCAGCAGAGGCACTAGAAGAAGGTGTCAAGGGCACCCAAGCAGCAGAAATTGTGGGTGATTGGGTGAAGCGTGCTAGGAATAGGGCTATCACGGAGCAAGCTCTGACACTTCTCGAATCATATGCCACATCCATTAGCGTCACATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mitofilin

Protein Analysis

618

Amino Acids

67.02

Weight (kDa)

4.76

Isoelectric Point (pI)

52.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mitofilin PF09731 401 - 613 1.3e-36 Mitochondrial inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39690
fragaria_vesca FvH4_1g21670 FvH4_1g21670 FvH4_1g21670
malus_domestica MD01G1023900.v1.1 MD15G1329400.v1.1
prunus_persica Prupe.6G178900_v2.0.a1
pyrus_communis pycom01g05150
rosa_chinensis RchiOBHm_Chr2g0115541
rosa_laevigata RLG00000018192
rosa_multiflora Rmu_ssc0000197.1_g000091
rosa_roxburghii Rroxscaffold_2G00128120 Rroxscaffold_2G00128990
rosa_rugosa Rorug02G0197700 Rorug02G0197800
rosa_samantha Rh2AG255100 Rh2BG266000 Rh2CG260300 Rh2DG263300
rosa_wichuraiana Rw2G019970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 589, 1742
AccII CGCG 2 cut(s) 34, 328
AciI CCGC 8 cut(s) 96, 99, 122, 135, 165, 204, 326, 1254
AclWI GGATC 3 cut(s) 46, 59, 1181
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 2 cut(s) 24, 1405
AcuI CTGAAG 6 cut(s) 564, 776, 993, 1034, 1537, 1713
AfaI GTAC 2 cut(s) 796, 1545
AfiI CCNNNNNNNGG 5 cut(s) 48, 251, 1536, 1571, 1574
AgeI ACCGGT 1 cut(s) 616
AhlI ACTAGT 1 cut(s) 581
AjnI CCWGG 2 cut(s) 1567, 1613
AloI GAACNNNNNNTCC 2 cut(s) 885, 917
AluBI AGCT 7 cut(s) 221, 230, 491, 710, 947, 1241, 1811
AluI AGCT 7 cut(s) 221, 230, 491, 710, 947, 1241, 1811
Alw21I GWGCWC 1 cut(s) 1170
Alw26I GTCTC 2 cut(s) 623, 776
Alw44I GTGCAC 1 cut(s) 1166
AlwI GGATC 3 cut(s) 46, 59, 1181
AlwNI CAGNNNCTG 1 cut(s) 1103
AoxI GGCC 3 cut(s) 39, 253, 840
ApaLI GTGCAC 1 cut(s) 1166
ApeKI GCWGC 9 cut(s) 119, 212, 265, 929, 1055, 1103, 1250, 1712, 1751
ApoI RAATTY 2 cut(s) 24, 1405
AsiGI ACCGGT 1 cut(s) 616
AspA2I CCTAGG 1 cut(s) 71
AspS9I GGNCC 5 cut(s) 12, 39, 137, 241, 1423
AsuC2I CCSGG 2 cut(s) 132, 141
AsuHPI GGTGA 3 cut(s) 1636, 1778, 1786
AvaII GGWCC 4 cut(s) 12, 137, 241, 1423
AvrII CCTAGG 1 cut(s) 71
AxyI CCTNAGG 2 cut(s) 366, 1440
BaeGI GKGCMC 4 cut(s) 594, 1170, 1318, 1745
BamHI GGATCC 1 cut(s) 51
BanI GGYRCC 2 cut(s) 589, 1742
BauI CACGAG 1 cut(s) 960
BbsI GAAGAC 1 cut(s) 693
Bbv12I GWGCWC 1 cut(s) 1170
BbvI GCAGC 9 cut(s) 106, 199, 277, 916, 1067, 1090, 1237, 1724, 1763
BccI CCATC 3 cut(s) 764, 884, 1643
BceAI ACGGC 1 cut(s) 26
BcgI CGANNNNNNTGC 2 cut(s) 1814, 1848
BciT130I CCWGG 2 cut(s) 1569, 1615
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 2 cut(s) 132, 141
BcoDI GTCTC 2 cut(s) 623, 776
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 6 cut(s) 72, 582, 1064, 1494, 1724, 1785
BlnI CCTAGG 1 cut(s) 71
BlpI GCTNAGC 1 cut(s) 126
Bme1390I CCNGG 4 cut(s) 132, 141, 1569, 1615
Bme18I GGWCC 4 cut(s) 12, 137, 241, 1423
BmgT120I GGNCC 5 cut(s) 12, 39, 137, 241, 1423
BmiI GGNNCC 6 cut(s) 53, 243, 273, 591, 666, 1744
BmrFI CCNGG 4 cut(s) 132, 141, 1569, 1615
BpiI GAAGAC 1 cut(s) 693
BpmI CTGGAG 1 cut(s) 1665
Bpu1102I GCTNAGC 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 1094, 1175
BpuMI CCSGG 2 cut(s) 132, 141
BsaJI CCNNGG 2 cut(s) 71, 594
BsaWI WCCGGW 3 cut(s) 151, 274, 616
BsaXI ACNNNNNCTCC 2 cut(s) 180, 210
Bsc4I CCNNNNNNNGG 5 cut(s) 48, 251, 1536, 1571, 1574
Bse118I RCCGGY 1 cut(s) 616
Bse1I ACTGG 2 cut(s) 1251, 1329
Bse21I CCTNAGG 2 cut(s) 366, 1440
Bse3DI GCAATG 1 cut(s) 1065
BseBI CCWGG 2 cut(s) 1569, 1615
BseDI CCNNGG 2 cut(s) 71, 594
BseGI GGATG 9 cut(s) 307, 409, 706, 766, 874, 1024, 1274, 1610, 1838
BseLI CCNNNNNNNGG 5 cut(s) 48, 251, 1536, 1571, 1574
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 8 cut(s) 140, 357, 366, 483, 618, 754, 939, 1431
BseNI ACTGG 2 cut(s) 1251, 1329
BseRI GAGGAG 5 cut(s) 392, 587, 614, 657, 1468
BseSI GKGCMC 4 cut(s) 594, 1170, 1318, 1745
BseX3I CGGCCG 1 cut(s) 253
BseXI GCAGC 9 cut(s) 106, 199, 277, 916, 1067, 1090, 1237, 1724, 1763
BsgI GTGCAG 2 cut(s) 597, 1089
Bsh1236I CGCG 2 cut(s) 34, 328
Bsh1285I CGRYCG 1 cut(s) 256
BshFI GGCC 3 cut(s) 41, 255, 842
BshNI GGYRCC 2 cut(s) 589, 1742
BshTI ACCGGT 1 cut(s) 616
BsiEI CGRYCG 1 cut(s) 256
BsiHKAI GWGCWC 1 cut(s) 1170
BsiSI CCGG 8 cut(s) 116, 132, 140, 152, 256, 269, 275, 617
BslFI GGGAC 1 cut(s) 1552
BslI CCNNNNNNNGG 5 cut(s) 48, 251, 1536, 1571, 1574
BsmAI GTCTC 2 cut(s) 623, 776
BsmFI GGGAC 1 cut(s) 1552
BsnI GGCC 3 cut(s) 41, 255, 842
Bsp1286I GDGCHC 4 cut(s) 594, 1170, 1318, 1745
Bsp143I GATC 4 cut(s) 51, 634, 1186, 1636
Bsp1720I GCTNAGC 1 cut(s) 126
BspACI CCGC 8 cut(s) 96, 99, 122, 135, 165, 204, 326, 1254
BspANI GGCC 3 cut(s) 41, 255, 842
BspCNI CTCAG 8 cut(s) 139, 358, 367, 484, 619, 753, 940, 1432
BspFNI CGCG 2 cut(s) 34, 328
BspLI GGNNCC 6 cut(s) 53, 243, 273, 591, 666, 1744
BspPI GGATC 3 cut(s) 46, 59, 1181
BspT107I GGYRCC 2 cut(s) 589, 1742
BsrDI GCAATG 1 cut(s) 1065
BsrFI RCCGGY 1 cut(s) 616
BsrI ACTGG 2 cut(s) 1251, 1329
BssAI RCCGGY 1 cut(s) 616
BssECI CCNNGG 2 cut(s) 71, 594
BssMI GATC 4 cut(s) 51, 634, 1186, 1636
BssSI CACGAG 1 cut(s) 960
BssT1I CCWWGG 2 cut(s) 71, 594
Bst2BI CACGAG 1 cut(s) 960
Bst2UI CCWGG 2 cut(s) 1569, 1615
Bst4CI ACNGT 1 cut(s) 1543
Bst6I CTCTTC 7 cut(s) 309, 333, 377, 456, 540, 1044, 1128
BstAPI GCANNNNNTGC 4 cut(s) 701, 926, 1103, 1165
BstC8I GCNNGC 8 cut(s) 146, 232, 918, 945, 1024, 1044, 1782, 1809
BstDEI CTNAG 9 cut(s) 126, 366, 375, 492, 627, 740, 948, 1440, 1516
BstEII GGTNACC 1 cut(s) 1347
BstF5I GGATG 9 cut(s) 307, 409, 706, 766, 874, 1024, 1274, 1610, 1838
BstFNI CGCG 2 cut(s) 34, 328
BstKTI GATC 4 cut(s) 54, 637, 1189, 1639
BstMAI GTCTC 2 cut(s) 623, 776
BstMBI GATC 4 cut(s) 51, 634, 1186, 1636
BstMCI CGRYCG 1 cut(s) 256
BstNI CCWGG 2 cut(s) 1569, 1615
BstPI GGTNACC 1 cut(s) 1347
BstSCI CCNGG 4 cut(s) 130, 139, 1567, 1613
BstSLI GKGCMC 4 cut(s) 594, 1170, 1318, 1745
BstUI CGCG 2 cut(s) 34, 328
BstV1I GCAGC 9 cut(s) 106, 199, 277, 916, 1067, 1090, 1237, 1724, 1763
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 1 cut(s) 51
BstYI RGATCY 1 cut(s) 51
BstZI CGGCCG 1 cut(s) 253
Bsu36I CCTNAGG 2 cut(s) 366, 1440
BsuRI GGCC 3 cut(s) 41, 255, 842
BtsCI GGATG 9 cut(s) 307, 409, 706, 766, 874, 1024, 1274, 1610, 1838
BtsI GCAGTG 2 cut(s) 784, 811
BtsIMutI CAGTG 5 cut(s) 484, 748, 784, 811, 1322
Cac8I GCNNGC 8 cut(s) 146, 232, 918, 945, 1024, 1044, 1782, 1809
CaiI CAGNNNCTG 1 cut(s) 1103
Cfr10I RCCGGY 1 cut(s) 616
Cfr13I GGNCC 5 cut(s) 12, 39, 137, 241, 1423
CpoI CGGWCCG 1 cut(s) 137
CseI GACGC 2 cut(s) 23, 1837
Csp6I GTAC 2 cut(s) 795, 1544
CspAI ACCGGT 1 cut(s) 616
CspI CGGWCCG 1 cut(s) 137
CviAII CATG 2 cut(s) 926, 1310
CviQI GTAC 2 cut(s) 795, 1544
DdeI CTNAG 9 cut(s) 126, 366, 375, 492, 627, 740, 948, 1440, 1516
DpnI GATC 4 cut(s) 53, 636, 1188, 1638
DpnII GATC 4 cut(s) 51, 634, 1186, 1636
EaeI YGGCCR 1 cut(s) 253
EagI CGGCCG 1 cut(s) 253
Eam1104I CTCTTC 7 cut(s) 309, 333, 377, 456, 540, 1044, 1128
EarI CTCTTC 7 cut(s) 309, 333, 377, 456, 540, 1044, 1128
EciI GGCGGA 1 cut(s) 85
EclXI CGGCCG 1 cut(s) 253
Eco130I CCWWGG 2 cut(s) 71, 594
Eco147I AGGCCT 1 cut(s) 842
Eco47I GGWCC 4 cut(s) 12, 137, 241, 1423
Eco52I CGGCCG 1 cut(s) 253
Eco57I CTGAAG 6 cut(s) 564, 776, 993, 1034, 1537, 1713
Eco81I CCTNAGG 2 cut(s) 366, 1440
Eco91I GGTNACC 1 cut(s) 1347
EcoO65I GGTNACC 1 cut(s) 1347
EcoRII CCWGG 2 cut(s) 1567, 1613
EcoT14I CCWWGG 2 cut(s) 71, 594
ErhI CCWWGG 2 cut(s) 71, 594
FaeI CATG 2 cut(s) 929, 1313
FalI AAGNNNNNCTT 6 cut(s) 1113, 1145, 1290, 1322, 1320, 1352
FaqI GGGAC 1 cut(s) 1552
FatI CATG 2 cut(s) 925, 1309
FauNDI CATATG 1 cut(s) 1831
FbaI TGATCA 1 cut(s) 634
FokI GGATG 9 cut(s) 314, 416, 713, 773, 881, 1031, 1261, 1597, 1825
FspBI CTAG 6 cut(s) 72, 582, 1064, 1494, 1724, 1785
GsuI CTGGAG 1 cut(s) 1665
HaeIII GGCC 3 cut(s) 41, 255, 842
HapII CCGG 8 cut(s) 116, 132, 140, 152, 256, 269, 275, 617
HgaI GACGC 2 cut(s) 23, 1837
Hin1II CATG 2 cut(s) 929, 1313
HinfI GANTC 6 cut(s) 112, 247, 449, 1483, 1658, 1826
HpaII CCGG 8 cut(s) 116, 132, 140, 152, 256, 269, 275, 617
HphI GGTGA 3 cut(s) 1636, 1778, 1786
Hpy166II GTNNAC 2 cut(s) 797, 1168
Hpy188I TCNGA 8 cut(s) 48, 376, 400, 544, 756, 973, 1488, 1815
Hpy8I GTNNAC 2 cut(s) 797, 1168
Hpy99I CGWCG 2 cut(s) 34, 39
HpyCH4III ACNGT 1 cut(s) 1543
HpyCH4IV ACGT 2 cut(s) 66, 483
HpyF3I CTNAG 9 cut(s) 126, 366, 375, 492, 627, 740, 948, 1440, 1516
HpySE526I ACGT 2 cut(s) 66, 483
Hsp92II CATG 2 cut(s) 929, 1313
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 4 cut(s) 51, 634, 1186, 1636
LmnI GCTCC 4 cut(s) 227, 367, 670, 1804
Lsp1109I GCAGC 9 cut(s) 106, 199, 277, 916, 1067, 1090, 1237, 1724, 1763
MaeI CTAG 6 cut(s) 72, 582, 1064, 1494, 1724, 1785
MaeII ACGT 2 cut(s) 66, 483
MaeIII GTNAC 5 cut(s) 479, 484, 1113, 1347, 1849
MalI GATC 4 cut(s) 53, 636, 1188, 1638
MboI GATC 4 cut(s) 51, 634, 1186, 1636
MfeI CAATTG 3 cut(s) 846, 1257, 1478
MflI RGATCY 1 cut(s) 51
MhlI GDGCHC 4 cut(s) 594, 1170, 1318, 1745
MlyI GAGTC 2 cut(s) 458, 1667
MmeI TCCRAC 3 cut(s) 438, 511, 846
MseI TTAA 6 cut(s) 179, 654, 989, 1076, 1214, 1532
MslI CAYNNNNRTG 1 cut(s) 1178
MspA1I CMGCKG 1 cut(s) 491
MspI CCGG 8 cut(s) 116, 132, 140, 152, 256, 269, 275, 617
MspR9I CCNGG 4 cut(s) 132, 141, 1569, 1615
MunI CAATTG 3 cut(s) 846, 1257, 1478
MvaI CCWGG 2 cut(s) 1569, 1615
MvnI CGCG 2 cut(s) 34, 328
NciI CCSGG 2 cut(s) 132, 141
NdeI CATATG 1 cut(s) 1831
NdeII GATC 4 cut(s) 51, 634, 1186, 1636
NlaIII CATG 2 cut(s) 929, 1313
NlaIV GGNNCC 6 cut(s) 53, 243, 273, 591, 666, 1744
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 3 cut(s) 479, 1113, 1849
PceI AGGCCT 1 cut(s) 842
PfeI GAWTC 4 cut(s) 112, 247, 1483, 1826
PinAI ACCGGT 1 cut(s) 616
PleI GAGTC 2 cut(s) 457, 1666
PpsI GAGTC 2 cut(s) 457, 1666
Psp6I CCWGG 2 cut(s) 1567, 1613
PspEI GGTNACC 1 cut(s) 1347
PspGI CCWGG 2 cut(s) 1567, 1613
PspN4I GGNNCC 6 cut(s) 53, 243, 273, 591, 666, 1744
PspPI GGNCC 5 cut(s) 12, 39, 137, 241, 1423
PstNI CAGNNNCTG 1 cut(s) 1103
PsuI RGATCY 1 cut(s) 51
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 2 cut(s) 796, 1545
RsaNI GTAC 2 cut(s) 795, 1544
RseI CAYNNNNRTG 1 cut(s) 1178
Rsr2I CGGWCCG 1 cut(s) 137
RsrII CGGWCCG 1 cut(s) 137
SaqAI TTAA 6 cut(s) 179, 654, 989, 1076, 1214, 1532
Sau3AI GATC 4 cut(s) 51, 634, 1186, 1636
Sau96I GGNCC 5 cut(s) 12, 39, 137, 241, 1423
SchI GAGTC 2 cut(s) 458, 1667
ScrFI CCNGG 4 cut(s) 132, 141, 1569, 1615
SduI GDGCHC 4 cut(s) 594, 1170, 1318, 1745
SgrAI CRCCGGYG 1 cut(s) 616
SinI GGWCC 4 cut(s) 12, 137, 241, 1423
SmiMI CAYNNNNRTG 1 cut(s) 1178
SmlI CTYRAG 2 cut(s) 1109, 1154
SmoI CTYRAG 2 cut(s) 1109, 1154
SpeI ACTAGT 1 cut(s) 581
SseBI AGGCCT 1 cut(s) 842
SsiI CCGC 8 cut(s) 96, 99, 122, 135, 165, 204, 326, 1254
SspMI CTAG 6 cut(s) 72, 582, 1064, 1494, 1724, 1785
StuI AGGCCT 1 cut(s) 842
StyD4I CCNGG 4 cut(s) 130, 139, 1567, 1613
StyI CCWWGG 2 cut(s) 71, 594
TaaI ACNGT 1 cut(s) 1543
TaiI ACGT 2 cut(s) 69, 486
TaqI TCGA 3 cut(s) 452, 1707, 1824
TatI WGTACW 1 cut(s) 794
TauI GCSGC 4 cut(s) 101, 125, 328, 1256
TfiI GAWTC 4 cut(s) 112, 247, 1483, 1826
Tru1I TTAA 6 cut(s) 179, 654, 989, 1076, 1214, 1532
Tru9I TTAA 6 cut(s) 179, 654, 989, 1076, 1214, 1532
TscAI CASTG 5 cut(s) 484, 748, 784, 811, 1329
TseFI GTSAC 3 cut(s) 479, 1113, 1849
TseI GCWGC 9 cut(s) 119, 212, 265, 929, 1055, 1103, 1250, 1712, 1751
Tsp45I GTSAC 3 cut(s) 479, 1113, 1849
TspDTI ATGAA 5 cut(s) 825, 914, 1017, 1223, 1298
TspGWI ACGGA 4 cut(s) 240, 1465, 1618, 1817
TspRI CASTG 5 cut(s) 484, 748, 784, 811, 1329
VneI GTGCAC 1 cut(s) 1166
VpaK11BI GGWCC 4 cut(s) 12, 137, 241, 1423
XapI RAATTY 2 cut(s) 24, 1405
XmaJI CCTAGG 1 cut(s) 71
XspI CTAG 6 cut(s) 72, 582, 1064, 1494, 1724, 1785
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.