Rroxscaffold_2G00128120

Belongs to the MICOS complex subunit Mic60 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0011414
Physical Location & Seq
Forward (+)
63705493 .. 63721297
15805 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00128120.1

Sequence Viewer

Length: 1884 bp
ATGTTGCGAAGGTCCATTCTCCAAATTTCGTCGCGTCGGGCCGTTCGGAGGATCCAAAGTCAGCACGTTCCTAGGTTTTTGAGAAAGGAGTTCTCCGCCGCAGTCCAGAAGAATCCGGCTGCGGCTCAGCCCGGCGGTCCGGGCAAGCCACCGGAGAAGTCTGGCGGTTCAAACGCTTTAATCTTTGGAGGTCTTGTTGTTGGCGGTGGGGCTGCTTTAGCTTACGGAGCTGGCTATCTGGACCCGATTCTCGGCCGGAAGAAGCAGCCGGAACCGGAAGTTGAAGTGATTGTCAATGAGGATGTAGGTGTGGAAGAGAATGTGCCGCGTGTGGAGGAAGAGAGTAGCAAACTGAACTTTGCTCCTGAGGTTTCTGAGGAGAAGAGTGATGCACATTCGGAGGATGTTGCCAAGTTTGAAGGTTTGGGTGGAAGTGAAGGCGAAAGCGAGTCGAAGTTGGAAGAGAAATCTGTGGTCAGTGACGTAACAGCTGAGAAGGATTTGGGGGAGGTTTCACAAAATGCAAATGTTGGAGTTTCGTCTGAAGAGAATGTAGATAATAAAAGTGTAGAGGAGAAAACTAGTGAGGTGCCCAAGGAGGAGGTTGAAACTGCACCGGTGTTTGCTGAGACTGATCAGCAAGAGAATGAGATTAAAGCATTGGCTCCTCAACAGGAAATCATAGAAGACAAGGCACAGGATGCAATAGCTAATGTTGAAGAACCACCAAGTTCTTTGCTCAGTGAATATAATCTGAAGGATGAGGCTGATGGAGGCAGTGAGACTATTATTAGTACACAAGGCAGTGATGAATACAATCCACTTCCTAAAGAAAAAGAGGCCTCAATTGGTGTTATTGAAGGGTTGGATGATGCTTACATATCCAAAGATGGAAAGTTAGTTCTTGACTTCTTGCAAGCAATTCATGCTGCTGAAAAAAGGCAAGCTGAGTTAGATGCTCGTGTTTCTTCTGAAGAAAAGAGAGCATTAAAGGAGAAGTATGAAAAGAAACTGAAGGATGCTGGCATTAGGGAAATGATGCTTGCAGAAGAGGCAGCAATGCTAGACAAGGAATTAAAGAGAGAAAGAGCAAAAGCAGTTGCTGCACTCAAGTCACTTCAAGAAAAACTTGAAGAGAAATATAAGACAGAACTTGAGCAGAAGGAAAACGAAGCAGAAATGAAGTTAAAAAAGGTTGAGGAATTAGCAAAAGCTGAACTGGCTGCCGCAATTGCAAGTGAGAAGGCATCCCAGATTGAGAAAATGACAGAAGCAAATCTTCATATAAATGCCTTGTGTGTTGCATTCTATGCAAGATCTGAAGAAGCTCGTCAGACTCACTCTGCTCACAAGCTTGCTTTGGGGGCACTTGCACTGGAAGATGCACTTTCCAAAGGGTTACCAATCCACACAGAAATAGAGGCTTTGGACACTAATCTTGAAGGCATTGATAAAAATTCAATTTTGGATTTGGTCCTGTCATCACTTCCTGAGGAAACACGGAGGAGTGGCACAGATACCTTGTTGCAATTGAATCAGAAGGCTAGTTATCTTCTTACTTCTTCCTTAGATTACCATGAAATATATCAATCTGGAGATGGGATTGAGGTGCTAGCCGTCAGTTGGATAAGCTCCGGTGAAAAATTCGACCGGGATTGGATCGGGGAAGAAGGAGATGATGTACGTAGTTTGTGTTGGGCAATAGACTCCTTTTATCAAGAGGGAGGATTTATCCTAGCTACATGTGCGTGCGAAGATGATATGAGAGGATCGAACCGTTCTTTTGTCTACGACGAAGAAACCCGGACATTCCGTAATCGCCGCGATGCAAGGAACGATCTTGGAGTGTTAGGTGGGATTGCAAATCTAAGAAAGAAATGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mitofilin

Protein Analysis

627

Amino Acids

68.91

Weight (kDa)

4.73

Isoelectric Point (pI)

59.28

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mitofilin PF09731 75 - 514 2.1e-25 Mitochondrial inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0011414)

Species Orthologous Gene IDs
arabidopsis_thaliana AT4G39690
fragaria_vesca FvH4_1g21670 FvH4_1g21670 FvH4_1g21670
malus_domestica MD01G1023900.v1.1 MD15G1329400.v1.1
prunus_persica Prupe.6G178900_v2.0.a1
pyrus_communis pycom01g05150
rosa_chinensis RchiOBHm_Chr2g0115541
rosa_laevigata RLG00000018192
rosa_multiflora Rmu_ssc0000197.1_g000091
rosa_roxburghii Rroxscaffold_2G00128120 Rroxscaffold_2G00128990
rosa_rugosa Rorug02G0197700 Rorug02G0197800
rosa_samantha Rh2AG255100 Rh2BG266000 Rh2CG260300 Rh2DG263300
rosa_wichuraiana Rw2G019970

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 589
AccI GTMKAC 1 cut(s) 1788
AccII CGCG 3 cut(s) 34, 328, 1824
AciI CCGC 9 cut(s) 96, 99, 122, 135, 165, 204, 326, 1227, 1822
AclWI GGATC 4 cut(s) 46, 59, 1667, 1777
AcoI YGGCCR 1 cut(s) 253
AcsI RAATTY 3 cut(s) 24, 1456, 1643
AcuI CTGAAG 5 cut(s) 564, 776, 993, 1034, 1341
AfaI GTAC 2 cut(s) 796, 1683
AfiI CCNNNNNNNGG 3 cut(s) 48, 251, 1623
AflIII ACRYGT 1 cut(s) 1742
AgeI ACCGGT 1 cut(s) 616
AhlI ACTAGT 1 cut(s) 581
AloI GAACNNNNNNTCC 2 cut(s) 885, 917
Alw26I GTCTC 2 cut(s) 623, 776
AlwI GGATC 4 cut(s) 46, 59, 1667, 1777
AlwNI CAGNNNCTG 1 cut(s) 1103
AoxI GGCC 3 cut(s) 39, 253, 840
ApeKI GCWGC 7 cut(s) 119, 212, 265, 929, 1055, 1103, 1223
ApoI RAATTY 3 cut(s) 24, 1456, 1643
AsiGI ACCGGT 1 cut(s) 616
AspA2I CCTAGG 1 cut(s) 71
AspS9I GGNCC 5 cut(s) 12, 39, 137, 241, 1474
AsuC2I CCSGG 4 cut(s) 132, 141, 1652, 1804
AsuHPI GGTGA 1 cut(s) 1649
AsuNHI GCTAGC 1 cut(s) 1612
AvaII GGWCC 4 cut(s) 12, 137, 241, 1474
AvrII CCTAGG 1 cut(s) 71
AxyI CCTNAGG 2 cut(s) 366, 1491
BaeGI GKGCMC 2 cut(s) 594, 1369
BamHI GGATCC 1 cut(s) 51
BanI GGYRCC 1 cut(s) 589
BauI CACGAG 1 cut(s) 960
BbsI GAAGAC 1 cut(s) 693
BbvI GCAGC 7 cut(s) 106, 199, 277, 916, 1067, 1090, 1210
BccI CCATC 3 cut(s) 764, 884, 1592
BceAI ACGGC 2 cut(s) 26, 1601
BclI TGATCA 1 cut(s) 634
BcnI CCSGG 4 cut(s) 132, 141, 1652, 1804
BcoDI GTCTC 2 cut(s) 623, 776
BcuI ACTAGT 1 cut(s) 581
BfaI CTAG 6 cut(s) 72, 582, 1064, 1545, 1613, 1736
BglII AGATCT 1 cut(s) 1316
BlnI CCTAGG 1 cut(s) 71
BlpI GCTNAGC 1 cut(s) 126
Bme1390I CCNGG 4 cut(s) 132, 141, 1652, 1804
Bme18I GGWCC 4 cut(s) 12, 137, 241, 1474
BmgT120I GGNCC 5 cut(s) 12, 39, 137, 241, 1474
BmiI GGNNCC 5 cut(s) 53, 243, 273, 591, 666
BmrFI CCNGG 4 cut(s) 132, 141, 1652, 1804
BmsI GCATC 9 cut(s) 379, 691, 862, 946, 1009, 1029, 1256, 1372, 1816
BmtI GCTAGC 1 cut(s) 1616
BpiI GAAGAC 1 cut(s) 693
BpmI CTGGAG 1 cut(s) 1614
Bpu1102I GCTNAGC 1 cut(s) 126
BpuEI CTTGAG 2 cut(s) 1094, 1175
BpuMI CCSGG 4 cut(s) 132, 141, 1652, 1804
BsaAI YACGTR 1 cut(s) 1685
BsaJI CCNNGG 2 cut(s) 71, 594
BsaWI WCCGGW 4 cut(s) 151, 274, 616, 1634
BsaXI ACNNNNNCTCC 4 cut(s) 180, 210, 1665, 1695
Bsc4I CCNNNNNNNGG 3 cut(s) 48, 251, 1623
Bse118I RCCGGY 1 cut(s) 616
Bse1I ACTGG 2 cut(s) 1224, 1380
Bse21I CCTNAGG 2 cut(s) 366, 1491
Bse3DI GCAATG 1 cut(s) 1065
BseDI CCNNGG 2 cut(s) 71, 594
BseGI GGATG 7 cut(s) 307, 409, 706, 766, 874, 1024, 1247
BseLI CCNNNNNNNGG 3 cut(s) 48, 251, 1623
BseMI GCAATG 1 cut(s) 1065
BseMII CTCAG 8 cut(s) 140, 357, 366, 483, 618, 754, 939, 1482
BseNI ACTGG 2 cut(s) 1224, 1380
BseRI GAGGAG 5 cut(s) 392, 587, 614, 657, 1519
BseSI GKGCMC 2 cut(s) 594, 1369
BseX3I CGGCCG 1 cut(s) 253
BseXI GCAGC 7 cut(s) 106, 199, 277, 916, 1067, 1090, 1210
BsgI GTGCAG 2 cut(s) 597, 1089
Bsh1236I CGCG 3 cut(s) 34, 328, 1824
Bsh1285I CGRYCG 2 cut(s) 256, 1651
BshFI GGCC 3 cut(s) 41, 255, 842
BshNI GGYRCC 1 cut(s) 589
BshTI ACCGGT 1 cut(s) 616
BsiEI CGRYCG 2 cut(s) 256, 1651
BslI CCNNNNNNNGG 3 cut(s) 48, 251, 1623
BsmAI GTCTC 2 cut(s) 623, 776
BsmI GAATGC 1 cut(s) 1304
BsnI GGCC 3 cut(s) 41, 255, 842
Bsp1286I GDGCHC 2 cut(s) 594, 1369
Bsp143I GATC 6 cut(s) 51, 634, 1316, 1659, 1769, 1837
Bsp1720I GCTNAGC 1 cut(s) 126
BspACI CCGC 9 cut(s) 96, 99, 122, 135, 165, 204, 326, 1227, 1822
BspANI GGCC 3 cut(s) 41, 255, 842
BspCNI CTCAG 8 cut(s) 139, 358, 367, 484, 619, 753, 940, 1483
BspFNI CGCG 3 cut(s) 34, 328, 1824
BspLI GGNNCC 5 cut(s) 53, 243, 273, 591, 666
BspOI GCTAGC 1 cut(s) 1616
BspPI GGATC 4 cut(s) 46, 59, 1667, 1777
BspT107I GGYRCC 1 cut(s) 589
BsrDI GCAATG 1 cut(s) 1065
BsrFI RCCGGY 1 cut(s) 616
BsrI ACTGG 2 cut(s) 1224, 1380
BssAI RCCGGY 1 cut(s) 616
BssECI CCNNGG 2 cut(s) 71, 594
BssMI GATC 6 cut(s) 51, 634, 1316, 1659, 1769, 1837
BssSI CACGAG 1 cut(s) 960
BssT1I CCWWGG 2 cut(s) 71, 594
Bst2BI CACGAG 1 cut(s) 960
Bst4CI ACNGT 1 cut(s) 1778
Bst6I CTCTTC 7 cut(s) 309, 333, 377, 456, 540, 1044, 1128
BstAPI GCANNNNNTGC 4 cut(s) 701, 926, 1103, 1310
BstBAI YACGTR 1 cut(s) 1685
BstC8I GCNNGC 9 cut(s) 146, 232, 918, 945, 1024, 1044, 1356, 1614, 1750
BstEII GGTNACC 1 cut(s) 1398
BstF5I GGATG 7 cut(s) 307, 409, 706, 766, 874, 1024, 1247
BstFNI CGCG 3 cut(s) 34, 328, 1824
BstKTI GATC 6 cut(s) 54, 637, 1319, 1662, 1772, 1840
BstMAI GTCTC 2 cut(s) 623, 776
BstMBI GATC 6 cut(s) 51, 634, 1316, 1659, 1769, 1837
BstMCI CGRYCG 2 cut(s) 256, 1651
BstNSI RCATGY 1 cut(s) 1746
BstPI GGTNACC 1 cut(s) 1398
BstSCI CCNGG 4 cut(s) 130, 139, 1650, 1802
BstSLI GKGCMC 2 cut(s) 594, 1369
BstSNI TACGTA 1 cut(s) 1685
BstUI CGCG 3 cut(s) 34, 328, 1824
BstV1I GCAGC 7 cut(s) 106, 199, 277, 916, 1067, 1090, 1210
BstV2I GAAGAC 1 cut(s) 693
BstX2I RGATCY 2 cut(s) 51, 1316
BstYI RGATCY 2 cut(s) 51, 1316
BstZI CGGCCG 1 cut(s) 253
Bsu36I CCTNAGG 2 cut(s) 366, 1491
BsuRI GGCC 3 cut(s) 41, 255, 842
BtgZI GCGATG 1 cut(s) 1839
BtsCI GGATG 7 cut(s) 307, 409, 706, 766, 874, 1024, 1247
BtsI GCAGTG 2 cut(s) 784, 811
BtsIMutI CAGTG 5 cut(s) 484, 748, 784, 811, 1373
Cac8I GCNNGC 9 cut(s) 146, 232, 918, 945, 1024, 1044, 1356, 1614, 1750
CaiI CAGNNNCTG 1 cut(s) 1103
Cfr10I RCCGGY 1 cut(s) 616
Cfr13I GGNCC 5 cut(s) 12, 39, 137, 241, 1474
CpoI CGGWCCG 1 cut(s) 137
CseI GACGC 1 cut(s) 23
Csp6I GTAC 2 cut(s) 795, 1682
CspAI ACCGGT 1 cut(s) 616
CspI CGGWCCG 1 cut(s) 137
CviAII CATG 3 cut(s) 926, 1577, 1743
CviQI GTAC 2 cut(s) 795, 1682
DpnI GATC 6 cut(s) 53, 636, 1318, 1661, 1771, 1839
DpnII GATC 6 cut(s) 51, 634, 1316, 1659, 1769, 1837
EaeI YGGCCR 1 cut(s) 253
EagI CGGCCG 1 cut(s) 253
Eam1104I CTCTTC 7 cut(s) 309, 333, 377, 456, 540, 1044, 1128
EarI CTCTTC 7 cut(s) 309, 333, 377, 456, 540, 1044, 1128
EciI GGCGGA 1 cut(s) 85
EclXI CGGCCG 1 cut(s) 253
Eco105I TACGTA 1 cut(s) 1685
Eco130I CCWWGG 2 cut(s) 71, 594
Eco147I AGGCCT 1 cut(s) 842
Eco47I GGWCC 4 cut(s) 12, 137, 241, 1474
Eco52I CGGCCG 1 cut(s) 253
Eco57I CTGAAG 5 cut(s) 564, 776, 993, 1034, 1341
Eco81I CCTNAGG 2 cut(s) 366, 1491
Eco91I GGTNACC 1 cut(s) 1398
EcoO65I GGTNACC 1 cut(s) 1398
EcoT14I CCWWGG 2 cut(s) 71, 594
ErhI CCWWGG 2 cut(s) 71, 594
FaeI CATG 3 cut(s) 929, 1580, 1746
FalI AAGNNNNNCTT 6 cut(s) 1113, 1145, 1263, 1295, 1371, 1403
FatI CATG 3 cut(s) 925, 1576, 1742
FbaI TGATCA 1 cut(s) 634
FblI GTMKAC 1 cut(s) 1788
FokI GGATG 7 cut(s) 314, 416, 713, 773, 881, 1031, 1234
FspBI CTAG 6 cut(s) 72, 582, 1064, 1545, 1613, 1736
GsuI CTGGAG 1 cut(s) 1614
HaeIII GGCC 3 cut(s) 41, 255, 842
HgaI GACGC 1 cut(s) 23
Hin1II CATG 3 cut(s) 929, 1580, 1746
HindIII AAGCTT 1 cut(s) 1352
HinfI GANTC 6 cut(s) 112, 247, 449, 1336, 1534, 1706
HphI GGTGA 1 cut(s) 1649
Hpy166II GTNNAC 2 cut(s) 797, 1789
Hpy188I TCNGA 9 cut(s) 48, 376, 400, 544, 756, 973, 1321, 1335, 1539
Hpy188III TCNNGA 9 cut(s) 106, 239, 365, 905, 1121, 1439, 1490, 1593, 1718
Hpy8I GTNNAC 2 cut(s) 797, 1789
Hpy99I CGWCG 3 cut(s) 34, 39, 1796
HpyCH4III ACNGT 1 cut(s) 1778
HpyCH4IV ACGT 3 cut(s) 66, 483, 1684
HpySE526I ACGT 3 cut(s) 66, 483, 1684
Hsp92II CATG 3 cut(s) 929, 1580, 1746
Ksp22I TGATCA 1 cut(s) 634
Kzo9I GATC 6 cut(s) 51, 634, 1316, 1659, 1769, 1837
LmnI GCTCC 4 cut(s) 227, 367, 670, 1637
Lsp1109I GCAGC 7 cut(s) 106, 199, 277, 916, 1067, 1090, 1210
LweI GCATC 9 cut(s) 379, 691, 862, 946, 1009, 1029, 1256, 1372, 1816
MaeI CTAG 6 cut(s) 72, 582, 1064, 1545, 1613, 1736
MaeII ACGT 3 cut(s) 66, 483, 1684
MaeIII GTNAC 4 cut(s) 479, 484, 1113, 1398
MalI GATC 6 cut(s) 53, 636, 1318, 1661, 1771, 1839
MboI GATC 6 cut(s) 51, 634, 1316, 1659, 1769, 1837
MfeI CAATTG 3 cut(s) 846, 1230, 1529
MflI RGATCY 2 cut(s) 51, 1316
MhlI GDGCHC 2 cut(s) 594, 1369
MlyI GAGTC 3 cut(s) 458, 1330, 1700
MmeI TCCRAC 4 cut(s) 438, 511, 846, 1604
MseI TTAA 5 cut(s) 179, 654, 989, 1076, 1187
MslI CAYNNNNRTG 2 cut(s) 1287, 1747
MspA1I CMGCKG 1 cut(s) 491
MspR9I CCNGG 4 cut(s) 132, 141, 1652, 1804
MunI CAATTG 3 cut(s) 846, 1230, 1529
Mva1269I GAATGC 1 cut(s) 1304
MvnI CGCG 3 cut(s) 34, 328, 1824
NciI CCSGG 4 cut(s) 132, 141, 1652, 1804
NdeII GATC 6 cut(s) 51, 634, 1316, 1659, 1769, 1837
NheI GCTAGC 1 cut(s) 1612
NlaIII CATG 3 cut(s) 929, 1580, 1746
NlaIV GGNNCC 5 cut(s) 53, 243, 273, 591, 666
NmeAIII GCCGAG 1 cut(s) 231
NmuCI GTSAC 2 cut(s) 479, 1113
NspI RCATGY 1 cut(s) 1746
PceI AGGCCT 1 cut(s) 842
PciI ACATGT 1 cut(s) 1742
PctI GAATGC 1 cut(s) 1304
PfeI GAWTC 3 cut(s) 112, 247, 1534
PinAI ACCGGT 1 cut(s) 616
PleI GAGTC 3 cut(s) 457, 1330, 1700
PpsI GAGTC 3 cut(s) 457, 1330, 1700
Ppu21I YACGTR 1 cut(s) 1685
PscI ACATGT 1 cut(s) 1742
PspEI GGTNACC 1 cut(s) 1398
PspN4I GGNNCC 5 cut(s) 53, 243, 273, 591, 666
PspPI GGNCC 5 cut(s) 12, 39, 137, 241, 1474
PstNI CAGNNNCTG 1 cut(s) 1103
PsuI RGATCY 2 cut(s) 51, 1316
PvuII CAGCTG 1 cut(s) 491
RsaI GTAC 2 cut(s) 796, 1683
RsaNI GTAC 2 cut(s) 795, 1682
RseI CAYNNNNRTG 2 cut(s) 1287, 1747
Rsr2I CGGWCCG 1 cut(s) 137
RsrII CGGWCCG 1 cut(s) 137
SaqAI TTAA 5 cut(s) 179, 654, 989, 1076, 1187
Sau3AI GATC 6 cut(s) 51, 634, 1316, 1659, 1769, 1837
Sau96I GGNCC 5 cut(s) 12, 39, 137, 241, 1474
SchI GAGTC 3 cut(s) 458, 1330, 1700
ScrFI CCNGG 4 cut(s) 132, 141, 1652, 1804
SduI GDGCHC 2 cut(s) 594, 1369
SfaNI GCATC 9 cut(s) 379, 691, 862, 946, 1009, 1029, 1256, 1372, 1816
SgrAI CRCCGGYG 1 cut(s) 616
SinI GGWCC 4 cut(s) 12, 137, 241, 1474
SmiMI CAYNNNNRTG 2 cut(s) 1287, 1747
SmlI CTYRAG 2 cut(s) 1109, 1154
SmoI CTYRAG 2 cut(s) 1109, 1154
SnaBI TACGTA 1 cut(s) 1685
SpeI ACTAGT 1 cut(s) 581
SseBI AGGCCT 1 cut(s) 842
SsiI CCGC 9 cut(s) 96, 99, 122, 135, 165, 204, 326, 1227, 1822
SspMI CTAG 6 cut(s) 72, 582, 1064, 1545, 1613, 1736
StuI AGGCCT 1 cut(s) 842
StyD4I CCNGG 4 cut(s) 130, 139, 1650, 1802
StyI CCWWGG 2 cut(s) 71, 594
TaaI ACNGT 1 cut(s) 1778
TaiI ACGT 3 cut(s) 69, 486, 1687
TaqI TCGA 3 cut(s) 452, 1647, 1772
TatI WGTACW 1 cut(s) 794
TauI GCSGC 5 cut(s) 101, 125, 328, 1229, 1824
TfiI GAWTC 3 cut(s) 112, 247, 1534
Tru1I TTAA 5 cut(s) 179, 654, 989, 1076, 1187
Tru9I TTAA 5 cut(s) 179, 654, 989, 1076, 1187
TscAI CASTG 5 cut(s) 484, 748, 784, 811, 1380
TseFI GTSAC 2 cut(s) 479, 1113
TseI GCWGC 7 cut(s) 119, 212, 265, 929, 1055, 1103, 1223
Tsp45I GTSAC 2 cut(s) 479, 1113
TspDTI ATGAA 6 cut(s) 825, 914, 1017, 1196, 1271, 1593
TspGWI ACGGA 3 cut(s) 240, 1516, 1802
TspRI CASTG 5 cut(s) 484, 748, 784, 811, 1380
VpaK11BI GGWCC 4 cut(s) 12, 137, 241, 1474
XapI RAATTY 3 cut(s) 24, 1456, 1643
XceI RCATGY 1 cut(s) 1746
XmaJI CCTAGG 1 cut(s) 71
XmiI GTMKAC 1 cut(s) 1788
XspI CTAG 6 cut(s) 72, 582, 1064, 1545, 1613, 1736
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.