Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0011371

Physical Location & Seq

Genomic Coordinates Forward (+)

13543942 .. 13547135

Gene Structure 3194 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1039700.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2499 bp

ATGTTGCAGAGAGCTGCAAGCAATGCATATTCATGGTGGTGGGCCAGCCACATCAGAACTAAGCAATCCAAATGGCTCGATCAAAACCTCCAAGATATGGAGGAAAAGGTGCAGAGTGTGCTTAAACTCATTGAAGAGGATGGAGACTCCTTTGCCAAGAGAGCAGAAATGTACTTCAAAAATAGACCGGAGCTGATACACTTCGTCGAAGAAACCTACAGAGCTTACCGGTCCTTAGCTGAGCGATATGATCACATTTCAACAGAGCTACAAAATGCCAACAACACAATCGCTTCTGTTTTCCCGGAACAAGTTCAGTTTGCCATGGATGACGAAGATGAATATCCCTCGACTAAAACGCCTAGAAGGCCTCCGGATATCTCAAAAGGGAACATCCCAAACGTTCCCCCGAAGGGTCCTACTAAAGATTTAAGGAGTCTTCTCACGAAAGCAACAGAGAAAAATATGCTGCAGCCTCAGCGCTCAATGAAATCCACGTCTGCTAAAACAGTTCCTAAATCCGGCTTGACCAAACCTCAGGCACGTGAAGAGATTGACAAGACGCAGAAACAGATTCTGTCCCTACAAACGGAGAAAGAGTTTGTAAAGAGTTCGTACGAGAATGGGCTTGCAAAGTACTGGGAAATTGAGAACCAAATAAAGGGAATGCAGGACAAAGTATGCGTTTTGCAAGATGAATTTGGTGATGGAACGGTCATTGAAGATGACGAGGCTCGAAATTTGATGGCAGCAGCTGCTTTAAAGTCATGCCAACAGACATTGGTTGATTTGCAGGCAAAACAGGAAACTACAGCTGAGGAAGCGAGGGTTGAGTCGAAAAGGGTTAAGGATGCCCGGAAGAACTTGGAGACTCTTAAGAATGAGTTTCATCCTGCTCAACCCAATCCGGAAAAGCCCAATGCAAACGATGAATCTGTAAATGCAGCAAATGAGAAAAGCTTGGACCAGGAAGTGGACAAGGCAGCCCATCAGAAGCAGAAATTAGAGTTGTTACGGGAGAAAATTAAGGAACACTATGACGCTGCACCAGATTCATCTCTCACTGTTTCGGAAATGGCAGAAAAGATTGACGAGCTCGTGACTAAGGTGATCAGTTTAGAGAGTGCAGTTTCATCACAGACTGCTCTAGTCAAGAGACTAAGAAGCGAAAATGATGAGATTCAAGAACAGATTAAAAATCTGGAAGGTGACAAGGCAACTCTCGTCGGTGACAAGAAAGACTTGAGCGAAAAGCTGAAGGAAATGGAGGAAAAGTTGCATCAAGTTCAGGATCTCAACCAAAGTGTTGAAAACCAGAATGACAATCTCCAGACACATTTTACTGAAGCGACTTGTAATCTAGATCACATTTCTCATAAACTTGAAGCCGTGAAGCCAGATGAGGAGGTCAAGGTCACAGGCTCATCAGAAACAAAAGAGGAATCTCTTGTCGAAACCAAGTTGCAGAAACAGCTTGAAGGAGAAGTAAATGCAGTTAATCCTGTTGCTGGATCCAAGGTACTGCAGGAAGCGAAGTCAGATGAGGAACTGAAGCCTACAGTTTCACTGCGAAAAGATGAAGAACTTCCGCCTGAATTGAGAGCATTAAAAGAGTCCACAGAACATAAGGAAATGGCAAAGTCTACTGATGGTTCTATAAAAGCTACAGATGCTCTCAGTACTTCTGCAGGAAAAGATGTTTCTCAATCCCTGGAAATTAAAAATGCGGTTAATCCTGTTGCTGAATCCAAGGTACTGCAGGAAGCGAAGTCAGATGAGGTTCAGCCTACAGTTTCGCTGCGAAAAGATGAAGAACTTCCACCTAAAGTGAGACCGTTAATAGAGTCAACCGAACATAAGGAAATGGAAAAGTCTACTGATGGTTCAGCTACAGATGCTCTCAGTACTTCTGCTGGCAATCAAGGAAAAGAAGTTTCTCAATCCCCGGAAATTGAAAAGTCAGTTAGTCCTGTTGCCGGCACCAAGGAAGTGCAGGAAACGAAGTCGGATGGGGAACTGAAGGCTACAGTTTCACATCAAAAAGAAGAAGAATCTCTGGATGAAGGGAGACTGTCCAAAGAGTCCATAGAGCATAAGCAAACAGAAAATCCTGCTGATGGTTCTCTAACAGCTACAGATGCTCCCAGTACTTCCGCTGGAAATCAAGGAAAAGATGTTTGTGAATCTCCGGAAATTAATAACGAAGTTGTGGAACAAGCAGCAAGGAACGAAGATGAGCCAGATTGGCAGAAATTGTTTGTGAATGGGATGGAGGGCAGAGAAAAGATTCTATTGACAGAGTACACAATAACTCTTCGGAACTACAAGGATCTGAAGAAGAAGTTCAGCGAAGCAGAGAACGACCTCTTTGAAACATCAGTGCAGATTAAAGAACTGAAGAGTGCCAGTGCCATGAAAGATGAAGAGATTAAATCATTGCATAAAAAACTGGATCTTCTGCTACAGAGTTTAGACCCCGACAAGTATTTCAGCAGATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

KIP1

Protein Analysis

833

Amino Acids

93.42

Weight (kDa)

4.99

Isoelectric Point (pI)

52.45

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KIP1	PF07765	11 - 84	2.3e-37	KIP1-like protein
NET2A	PF25014	230 - 464	1.2e-95	NET2A-like domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0011371)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G22560
fragaria_vesca	FvH4_1g19623
malus_domestica	MD01G1039700.v1.1 MD15G1312100.v1.1
prunus_persica	Prupe.6G201900_v2.0.a1
pyrus_communis	pycom01g06730 pycom01g06750
rosa_chinensis	RchiOBHm_Chr2g0111181
rosa_laevigata	RLG00000017871
rosa_multiflora	Rmu_co8252337.1_g000001 Rmu_sc0033572.1_g000001 Rmu_sc0033573.1_g000001 Rmu_ssc0000102.1_g000003
rosa_roxburghii	Rroxscaffold_2G00132650
rosa_rugosa	Rorug02G0171300
rosa_samantha	Rh2AG224300 Rh2BG236000 Rh2CG227400 Rh2DG232000
rosa_wichuraiana	Rw2G017320

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	1 cut(s)	1979
AccB7I	CCANNNNNTGG	2 cut(s)	97, 973
AccI	GTMKAC	2 cut(s)	1643, 1874
AccIII	TCCGGA	3 cut(s)	373, 907, 2188
AciI	CCGC	3 cut(s)	1589, 1727, 2154
AclI	AACGTT	1 cut(s)	402
AclWI	GGATC	5 cut(s)	1299, 1506, 1519, 2337, 2460
AcsI	RAATTY	2 cut(s)	698, 739
AcuI	CTGAAG	6 cut(s)	1277, 1365, 1571, 2039, 2354, 2417
AcvI	CACGTG	1 cut(s)	545
AfaI	GTAC	9 cut(s)	173, 617, 638, 1521, 1681, 1755, 1906, 2149, 2303
AfeI	AGCGCT	1 cut(s)	482
AfiI	CCNNNNNNNGG	9 cut(s)	97, 413, 521, 589, 661, 973, 1508, 1976, 2117
AflII	CTTAAG	1 cut(s)	875
AgeI	ACCGGT	1 cut(s)	228
AjiI	CACGTC	1 cut(s)	498
AjnI	CCWGG	2 cut(s)	966, 1710
Alw21I	GWGCWC	1 cut(s)	1098
Alw26I	GTCTC	5 cut(s)	138, 863, 1150, 1825, 2062
AlwI	GGATC	5 cut(s)	1299, 1506, 1519, 2337, 2460
AlwNI	CAGNNNCTG	2 cut(s)	577, 755
Aor13HI	TCCGGA	3 cut(s)	373, 907, 2188
Aor51HI	AGCGCT	1 cut(s)	482
AoxI	GGCC	2 cut(s)	42, 368
ApoI	RAATTY	2 cut(s)	698, 739
AseI	ATTAAT	1 cut(s)	2196
AsiGI	ACCGGT	1 cut(s)	228
Asp700I	GAANNNNTTC	5 cut(s)	312, 1584, 1815, 2286, 2342
AspLEI	GCGC	1 cut(s)	483
AspS9I	GGNCC	4 cut(s)	42, 231, 416, 964
AsuC2I	CCSGG	3 cut(s)	305, 856, 1946
AsuHPI	GGTGA	4 cut(s)	716, 1120, 1220, 1241
AvaII	GGWCC	3 cut(s)	231, 416, 964
AxyI	CCTNAGG	1 cut(s)	537
BamHI	GGATCC	1 cut(s)	1511
BanI	GGYRCC	1 cut(s)	1979
BanII	GRGCYC	1 cut(s)	1098
BauI	CACGAG	1 cut(s)	1097
BbrPI	CACGTG	1 cut(s)	545
BbsI	GAAGAC	1 cut(s)	431
Bbv12I	GWGCWC	1 cut(s)	1098
BbvCI	CCTCAGC	2 cut(s)	477, 816
BccI	CCATC	9 cut(s)	134, 701, 739, 996, 1643, 1874, 2003, 2111, 2263
BceAI	ACGGC	1 cut(s)	1373
BciT130I	CCWGG	2 cut(s)	968, 1712
BclI	TGATCA	2 cut(s)	250, 1110
BcnI	CCSGG	3 cut(s)	305, 856, 1946
BcoDI	GTCTC	5 cut(s)	138, 863, 1150, 1825, 2062
BfaI	CTAG	3 cut(s)	363, 1148, 1361
BfoI	RGCGCY	1 cut(s)	484
BfrI	CTTAAG	1 cut(s)	875
BglI	GCCNNNNNGGC	2 cut(s)	367, 2245
BlpI	GCTNAGC	1 cut(s)	240
BmcAI	AGTACT	4 cut(s)	638, 1681, 1906, 2149
Bme1390I	CCNGG	5 cut(s)	305, 856, 968, 1712, 1946
Bme18I	GGWCC	3 cut(s)	231, 416, 964
BmgBI	CACGTC	1 cut(s)	498
BmgT120I	GGNCC	4 cut(s)	42, 231, 416, 964
BmiI	GGNNCC	3 cut(s)	417, 1513, 1981
BmrFI	CCNGG	5 cut(s)	305, 856, 968, 1712, 1946
BmrI	ACTGGG	2 cut(s)	649, 2139
BmsI	GCATC	5 cut(s)	841, 1288, 1660, 1885, 2128
BmuI	ACTGGG	2 cut(s)	649, 2139
BpiI	GAAGAC	1 cut(s)	431
BpmI	CTGGAG	1 cut(s)	1313
Bpu10I	CCTNAGC	3 cut(s)	235, 477, 816
Bpu1102I	GCTNAGC	1 cut(s)	240
BpuEI	CTTGAG	1 cut(s)	1264
BpuMI	CCSGG	3 cut(s)	305, 856, 1946
BsaAI	YACGTR	1 cut(s)	545
BsaBI	GATNNNNATC	1 cut(s)	342
BsaI	GGTCTC	1 cut(s)	1825
BsaJI	CCNNGG	6 cut(s)	324, 1515, 1710, 1749, 1944, 1983
BsaWI	WCCGGW	5 cut(s)	187, 228, 373, 907, 2188
BsaXI	ACNNNNNCTCC	6 cut(s)	584, 614, 1259, 1289, 2125, 2155
Bsc4I	CCNNNNNNNGG	9 cut(s)	97, 413, 521, 589, 661, 973, 1508, 1976, 2117
Bse118I	RCCGGY	2 cut(s)	228, 1976
Bse1I	ACTGG	4 cut(s)	644, 2145, 2406, 2454
Bse21I	CCTNAGG	1 cut(s)	537
Bse3DI	GCAATG	2 cut(s)	28, 2435
Bse8I	GATNNNNATC	1 cut(s)	342
BseAI	TCCGGA	3 cut(s)	373, 907, 2188
BseBI	CCWGG	2 cut(s)	968, 1712
BseDI	CCNNGG	6 cut(s)	324, 1515, 1710, 1749, 1944, 1983
BseGI	GGATG	8 cut(s)	145, 334, 393, 856, 889, 2014, 2065, 2274
BseJI	GATNNNNATC	1 cut(s)	342
BseLI	CCNNNNNNNGG	9 cut(s)	97, 413, 521, 589, 661, 973, 1508, 1976, 2117
BseMI	GCAATG	2 cut(s)	28, 2435
BseMII	CTCAG	6 cut(s)	231, 491, 551, 807, 1690, 1915
BseNI	ACTGG	4 cut(s)	644, 2145, 2406, 2454
BseRI	GAGGAG	1 cut(s)	1418
BsgI	GTGCAG	5 cut(s)	131, 1029, 1146, 2012, 2402
BshFI	GGCC	2 cut(s)	44, 370
BshNI	GGYRCC	1 cut(s)	1979
BshTI	ACCGGT	1 cut(s)	228
BsiHKAI	GWGCWC	1 cut(s)	1098
BsiWI	CGTACG	1 cut(s)	615
BslFI	GGGAC	1 cut(s)	565
BslI	CCNNNNNNNGG	9 cut(s)	97, 413, 521, 589, 661, 973, 1508, 1976, 2117
BsmAI	GTCTC	5 cut(s)	138, 863, 1150, 1825, 2062
BsmFI	GGGAC	1 cut(s)	565
BsmI	GAATGC	1 cut(s)	672
BsnI	GGCC	2 cut(s)	44, 370
Bso31I	GGTCTC	1 cut(s)	1825
Bsp1286I	GDGCHC	1 cut(s)	1098
Bsp13I	TCCGGA	3 cut(s)	373, 907, 2188
Bsp143I	GATC	8 cut(s)	79, 250, 1110, 1291, 1363, 1511, 2329, 2452
Bsp1720I	GCTNAGC	1 cut(s)	240
Bsp19I	CCATGG	1 cut(s)	324
BspACI	CCGC	3 cut(s)	1589, 1727, 2154
BspANI	GGCC	2 cut(s)	44, 370
BspCNI	CTCAG	6 cut(s)	232, 490, 550, 808, 1689, 1914
BspEI	TCCGGA	3 cut(s)	373, 907, 2188
BspLI	GGNNCC	3 cut(s)	417, 1513, 1981
BspMAI	CTGCAG	4 cut(s)	474, 1527, 1690, 1761
BspPI	GGATC	5 cut(s)	1299, 1506, 1519, 2337, 2460
BspT107I	GGYRCC	1 cut(s)	1979
BspTI	CTTAAG	1 cut(s)	875
BspTNI	GGTCTC	1 cut(s)	1825
BsrDI	GCAATG	2 cut(s)	28, 2435
BsrFI	RCCGGY	2 cut(s)	228, 1976
BsrI	ACTGG	4 cut(s)	644, 2145, 2406, 2454
BssAI	RCCGGY	2 cut(s)	228, 1976
BssECI	CCNNGG	6 cut(s)	324, 1515, 1710, 1749, 1944, 1983
BssMI	GATC	8 cut(s)	79, 250, 1110, 1291, 1363, 1511, 2329, 2452
BssSI	CACGAG	1 cut(s)	1097
BssT1I	CCWWGG	4 cut(s)	324, 1515, 1749, 1983
Bst2BI	CACGAG	1 cut(s)	1097
Bst2UI	CCWGG	2 cut(s)	968, 1712
Bst4CI	ACNGT	8 cut(s)	511, 715, 1066, 1561, 1792, 1836, 2029, 2073
Bst6I	CTCTTC	5 cut(s)	129, 543, 2319, 2393, 2418
BstAFI	CTTAAG	1 cut(s)	875
BstAPI	GCANNNNNTGC	3 cut(s)	23, 118, 755
BstBAI	YACGTR	1 cut(s)	545
BstC8I	GCNNGC	6 cut(s)	19, 46, 630, 795, 1915, 1978
BstDSI	CCRYGG	1 cut(s)	324
BstF5I	GGATG	8 cut(s)	145, 334, 393, 856, 889, 2014, 2065, 2274
BstH2I	RGCGCY	1 cut(s)	484
BstHHI	GCGC	1 cut(s)	483
BstKTI	GATC	8 cut(s)	82, 253, 1113, 1294, 1366, 1514, 2332, 2455
BstMAI	GTCTC	5 cut(s)	138, 863, 1150, 1825, 2062
BstMBI	GATC	8 cut(s)	79, 250, 1110, 1291, 1363, 1511, 2329, 2452
BstNI	CCWGG	2 cut(s)	968, 1712
BstSCI	CCNGG	5 cut(s)	303, 854, 966, 1710, 1944
BstV2I	GAAGAC	1 cut(s)	431
BstX2I	RGATCY	4 cut(s)	1291, 1511, 2329, 2452
BstYI	RGATCY	4 cut(s)	1291, 1511, 2329, 2452
Bsu36I	CCTNAGG	1 cut(s)	537
BsuRI	GGCC	2 cut(s)	44, 370
BtgI	CCRYGG	1 cut(s)	324
BtrI	CACGTC	1 cut(s)	498
BtsCI	GGATG	8 cut(s)	145, 334, 393, 856, 889, 2014, 2065, 2274
BtsI	GCAGTG	1 cut(s)	1565
BtsIMutI	CAGTG	4 cut(s)	1062, 1565, 2385, 2413
Cac8I	GCNNGC	6 cut(s)	19, 46, 630, 795, 1915, 1978
CaiI	CAGNNNCTG	2 cut(s)	577, 755
CfoI	GCGC	1 cut(s)	483
Cfr10I	RCCGGY	2 cut(s)	228, 1976
Cfr13I	GGNCC	4 cut(s)	42, 231, 416, 964
CseI	GACGC	2 cut(s)	571, 1049
Csp6I	GTAC	9 cut(s)	172, 616, 637, 1520, 1680, 1754, 1905, 2148, 2302
CspAI	ACCGGT	1 cut(s)	228
CviAII	CATG	4 cut(s)	33, 325, 768, 2413
CviQI	GTAC	9 cut(s)	172, 616, 637, 1520, 1680, 1754, 1905, 2148, 2302
DpnI	GATC	8 cut(s)	81, 252, 1112, 1293, 1365, 1513, 2331, 2454
DpnII	GATC	8 cut(s)	79, 250, 1110, 1291, 1363, 1511, 2329, 2452
DraI	TTTAAA	1 cut(s)	762
Eam1104I	CTCTTC	5 cut(s)	129, 543, 2319, 2393, 2418
EarI	CTCTTC	5 cut(s)	129, 543, 2319, 2393, 2418
EciI	GGCGGA	1 cut(s)	1578
Ecl136II	GAGCTC	1 cut(s)	1096
Eco130I	CCWWGG	4 cut(s)	324, 1515, 1749, 1983
Eco147I	AGGCCT	1 cut(s)	370
Eco24I	GRGCYC	1 cut(s)	1098
Eco31I	GGTCTC	1 cut(s)	1825
Eco32I	GATATC	1 cut(s)	379
Eco47I	GGWCC	3 cut(s)	231, 416, 964
Eco47III	AGCGCT	1 cut(s)	482
Eco53kI	GAGCTC	1 cut(s)	1096
Eco57I	CTGAAG	6 cut(s)	1277, 1365, 1571, 2039, 2354, 2417
Eco72I	CACGTG	1 cut(s)	545
Eco81I	CCTNAGG	1 cut(s)	537
EcoICRI	GAGCTC	1 cut(s)	1096
EcoO109I	RGGNCCY	1 cut(s)	416
EcoRII	CCWGG	2 cut(s)	966, 1710
EcoRV	GATATC	1 cut(s)	379
EcoT14I	CCWWGG	4 cut(s)	324, 1515, 1749, 1983
EcoT22I	ATGCAT	1 cut(s)	28
EcoT38I	GRGCYC	1 cut(s)	1098
ErhI	CCWWGG	4 cut(s)	324, 1515, 1749, 1983
FaeI	CATG	4 cut(s)	36, 328, 771, 2416
FalI	AAGNNNNNCTT	2 cut(s)	1226, 1258
FaqI	GGGAC	1 cut(s)	565
FatI	CATG	4 cut(s)	32, 324, 767, 2412
FbaI	TGATCA	2 cut(s)	250, 1110
FblI	GTMKAC	2 cut(s)	1643, 1874
FokI	GGATG	8 cut(s)	152, 341, 380, 863, 876, 2021, 2072, 2281
FriOI	GRGCYC	1 cut(s)	1098
FspBI	CTAG	3 cut(s)	363, 1148, 1361
GlaI	GCGC	1 cut(s)	482
GsuI	CTGGAG	1 cut(s)	1313
HaeII	RGCGCY	1 cut(s)	484
HaeIII	GGCC	2 cut(s)	44, 370
HgaI	GACGC	2 cut(s)	571, 1049
HhaI	GCGC	1 cut(s)	483
Hin1II	CATG	4 cut(s)	36, 328, 771, 2416
Hin6I	GCGC	1 cut(s)	481
HinP1I	GCGC	1 cut(s)	481
HincII	GTYRAC	1 cut(s)	1848
HindII	GTYRAC	1 cut(s)	1848
HindIII	AAGCTT	1 cut(s)	958
HphI	GGTGA	4 cut(s)	716, 1120, 1220, 1241
Hpy166II	GTNNAC	6 cut(s)	976, 1617, 1644, 1848, 1875, 2304
Hpy188I	TCNGA	9 cut(s)	56, 993, 1072, 1429, 1540, 1774, 2008, 2319, 2334
Hpy8I	GTNNAC	6 cut(s)	976, 1617, 1644, 1848, 1875, 2304
Hpy99I	CGWCG	2 cut(s)	209, 1229
HpyAV	CCTTC	7 cut(s)	360, 406, 1199, 1252, 1472, 2014, 2057
HpyCH4III	ACNGT	8 cut(s)	511, 715, 1066, 1561, 1792, 1836, 2029, 2073
HpyCH4IV	ACGT	3 cut(s)	402, 497, 544
HpySE526I	ACGT	3 cut(s)	402, 497, 544
Hsp92II	CATG	4 cut(s)	36, 328, 771, 2416
HspAI	GCGC	1 cut(s)	481
Kpn2I	TCCGGA	3 cut(s)	373, 907, 2188
KroI	GCCGGC	1 cut(s)	1976
KroNI	GCCGGC	1 cut(s)	1978
Ksp22I	TGATCA	2 cut(s)	250, 1110
Kzo9I	GATC	8 cut(s)	79, 250, 1110, 1291, 1363, 1511, 2329, 2452
LmnI	GCTCC	2 cut(s)	190, 2146
LweI	GCATC	5 cut(s)	841, 1288, 1660, 1885, 2128
MaeI	CTAG	3 cut(s)	363, 1148, 1361
MaeII	ACGT	3 cut(s)	402, 497, 544
MaeIII	GTNAC	5 cut(s)	1011, 1099, 1208, 1229, 1414
MalI	GATC	8 cut(s)	81, 252, 1112, 1293, 1365, 1513, 2331, 2454
MboI	GATC	8 cut(s)	79, 250, 1110, 1291, 1363, 1511, 2329, 2452
MflI	RGATCY	4 cut(s)	1291, 1511, 2329, 2452
MhlI	GDGCHC	1 cut(s)	1098
MlyI	GAGTC	7 cut(s)	140, 445, 842, 865, 1622, 1853, 2090
MmeI	TCCRAC	1 cut(s)	1986
Mph1103I	ATGCAT	1 cut(s)	28
MroI	TCCGGA	3 cut(s)	373, 907, 2188
MroNI	GCCGGC	1 cut(s)	1976
MroXI	GAANNNNTTC	5 cut(s)	312, 1584, 1815, 2286, 2342
MslI	CAYNNNNRTG	2 cut(s)	31, 37
MspA1I	CMGCKG	3 cut(s)	755, 815, 2156
MspCI	CTTAAG	1 cut(s)	875
MspR9I	CCNGG	5 cut(s)	305, 856, 968, 1712, 1946
Mva1269I	GAATGC	1 cut(s)	672
MvaI	CCWGG	2 cut(s)	968, 1712
NaeI	GCCGGC	1 cut(s)	1978
NciI	CCSGG	3 cut(s)	305, 856, 1946
NcoI	CCATGG	1 cut(s)	324
NdeII	GATC	8 cut(s)	79, 250, 1110, 1291, 1363, 1511, 2329, 2452
NgoMIV	GCCGGC	1 cut(s)	1976
NlaIII	CATG	4 cut(s)	36, 328, 771, 2416
NlaIV	GGNNCC	3 cut(s)	417, 1513, 1981
NmuCI	GTSAC	4 cut(s)	1099, 1208, 1229, 1414
NsiI	ATGCAT	1 cut(s)	28
PceI	AGGCCT	1 cut(s)	370
PctI	GAATGC	1 cut(s)	672
PdiI	GCCGGC	1 cut(s)	1978
PdmI	GAANNNNTTC	5 cut(s)	312, 1584, 1815, 2286, 2342
PfeI	GAWTC	9 cut(s)	574, 932, 1052, 1180, 1442, 1745, 2051, 2183, 2287
Pfl23II	CGTACG	1 cut(s)	615
PflMI	CCANNNNNTGG	2 cut(s)	97, 973
PfoI	TCCNGGA	1 cut(s)	303
PinAI	ACCGGT	1 cut(s)	228
PleI	GAGTC	7 cut(s)	140, 444, 841, 865, 1621, 1852, 2089
PmaCI	CACGTG	1 cut(s)	545
PmlI	CACGTG	1 cut(s)	545
PpsI	GAGTC	7 cut(s)	140, 444, 841, 865, 1621, 1852, 2089
Ppu21I	YACGTR	1 cut(s)	545
PpuMI	RGGWCCY	1 cut(s)	416
PshBI	ATTAAT	1 cut(s)	2196
Psp124BI	GAGCTC	1 cut(s)	1098
Psp1406I	AACGTT	1 cut(s)	402
Psp5II	RGGWCCY	1 cut(s)	416
Psp6I	CCWGG	2 cut(s)	966, 1710
PspCI	CACGTG	1 cut(s)	545
PspGI	CCWGG	2 cut(s)	966, 1710
PspLI	CGTACG	1 cut(s)	615
PspN4I	GGNNCC	3 cut(s)	417, 1513, 1981
PspPI	GGNCC	4 cut(s)	42, 231, 416, 964
PspPPI	RGGWCCY	1 cut(s)	416
PstI	CTGCAG	4 cut(s)	474, 1527, 1690, 1761
PstNI	CAGNNNCTG	2 cut(s)	577, 755
PsuI	RGATCY	4 cut(s)	1291, 1511, 2329, 2452
PvuII	CAGCTG	2 cut(s)	755, 815
RsaI	GTAC	9 cut(s)	173, 617, 638, 1521, 1681, 1755, 1906, 2149, 2303
RsaNI	GTAC	9 cut(s)	172, 616, 637, 1520, 1680, 1754, 1905, 2148, 2302
RseI	CAYNNNNRTG	2 cut(s)	31, 37
SacI	GAGCTC	1 cut(s)	1098
Sau3AI	GATC	8 cut(s)	79, 250, 1110, 1291, 1363, 1511, 2329, 2452
Sau96I	GGNCC	4 cut(s)	42, 231, 416, 964
ScaI	AGTACT	4 cut(s)	638, 1681, 1906, 2149
SchI	GAGTC	7 cut(s)	140, 445, 842, 865, 1622, 1853, 2090
ScrFI	CCNGG	5 cut(s)	305, 856, 968, 1712, 1946
SduI	GDGCHC	1 cut(s)	1098
SfaNI	GCATC	5 cut(s)	841, 1288, 1660, 1885, 2128
SinI	GGWCC	3 cut(s)	231, 416, 964
SmiMI	CAYNNNNRTG	2 cut(s)	31, 37
SmlI	CTYRAG	2 cut(s)	875, 1243
SmoI	CTYRAG	2 cut(s)	875, 1243
SseBI	AGGCCT	1 cut(s)	370
SsiI	CCGC	3 cut(s)	1589, 1727, 2154
SspMI	CTAG	3 cut(s)	363, 1148, 1361
SstI	GAGCTC	1 cut(s)	1098
StuI	AGGCCT	1 cut(s)	370
StyD4I	CCNGG	5 cut(s)	303, 854, 966, 1710, 1944
StyI	CCWWGG	4 cut(s)	324, 1515, 1749, 1983
TaaI	ACNGT	8 cut(s)	511, 715, 1066, 1561, 1792, 1836, 2029, 2073
TaiI	ACGT	3 cut(s)	405, 500, 547
TaqI	TCGA	6 cut(s)	78, 207, 350, 736, 836, 1452
TatI	WGTACW	6 cut(s)	171, 636, 1679, 1904, 2147, 2301
TfiI	GAWTC	9 cut(s)	574, 932, 1052, 1180, 1442, 1745, 2051, 2183, 2287
TscAI	CASTG	4 cut(s)	1069, 1572, 2385, 2413
TseFI	GTSAC	4 cut(s)	1099, 1208, 1229, 1414
Tsp45I	GTSAC	4 cut(s)	1099, 1208, 1229, 1414
TspGWI	ACGGA	1 cut(s)	605
TspRI	CASTG	4 cut(s)	1069, 1572, 2385, 2413
Van91I	CCANNNNNTGG	2 cut(s)	97, 973
Vha464I	CTTAAG	1 cut(s)	875
VpaK11BI	GGWCC	3 cut(s)	231, 416, 964
VspI	ATTAAT	1 cut(s)	2196
XapI	RAATTY	2 cut(s)	698, 739
XbaI	TCTAGA	1 cut(s)	1360
XmiI	GTMKAC	2 cut(s)	1643, 1874
XmnI	GAANNNNTTC	5 cut(s)	312, 1584, 1815, 2286, 2342
XspI	CTAG	3 cut(s)	363, 1148, 1361
ZrmI	AGTACT	4 cut(s)	638, 1681, 1906, 2149
Zsp2I	ATGCAT	1 cut(s)	28

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.