Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0014641

Physical Location & Seq

Genomic Coordinates Reverse (-)

13631628 .. 13634610

Gene Structure 2983 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1040600.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1251 bp

ATGGCGAAGTCACAAGCGAAGGTCCAGCTGGAGAATGAAGTGATGAAACGTGTAGACATGGTCAGAGACATTGAAGTACTACGACGAGAAAGAGATGTTTTTCGAAGAAGGATTGAATTTTGTAGACAAAAGGATGCCACAGAAACGACAGAAGGGTTGAGTGAATTGAGCTGCTGTCTCAGAGAGTACACAGATGAGGAGATCAAACTGGCCACCGACAAATTTTCAGAGCGTTTTAGATTGAAATCAGGAATTGATTTGACCAGTGTTTATAAAGGGCGTATCAATCATGTCACAGTAGCTATCAAAGTTCTTAACTCAGCTTATGGACTGTCCCAAGAAGATTTTCAGGCTAAGGTGAAGCTCATCAGCGACATTAGACATCCTCATGTGCTTGCTATGATGGGCTACTGCTCCCAGCCAAACTCTACTGTCTTCGAATACATGCACAATGGTAGCTTACGGGACATCTTAGTTTCCTCCCATGAAATAAAGAACCTCGCCCGGGACCTTTGGTGGCACGACCGCATCCGCATTGCAGCGGAGATTTGCTCCGCAGTATGCTACCTCCACATGGCTCGGCCGAAACCCATTGTTCATGGTCACCTCAGCCCATCGAACATTCTCCTCGACCGCAATCTCGTGGTCAAGGTCAGTGGTTTAGGGCTCAACCAATCCTTGTACGACGAAAGCCACATTGTATGGGACATAAGGGCTTTTGGGATTTTGACTCTCAATCTTCTAACTGGAAGGAACTGGGTTGATGAGGATATGCTAATGGACAAGGAAGGATTAGTTCGAGTTTTAGATAAGAAGGCTGGGCAATGGCCATTGGATTTGGCAGAGGAACTTGTGAGCTTGGTGTTAAGGTGTTTAACCATTACCAATGGACCCAATAGGGATTTAAGGAGAATAATGGAGGAGCTTGGTGAAATCAGAAAAAGGGCTGATGTTTTAGTAGCAAGAGGAGGATCTGGCTTGAATATTAAGGGAATTGTTCATGGAGAGGTCACCAATGATGTCCCAAGTGTTTTCATATGCCCCATATTTCAGGAAGTAATGAAGGATCCACATATAGCGGCTGATGGATTTTCGTACGAGCAAGAAGCCATAGGAAAATGGTTGCGACTGGGGCATGATACATCGCCGATGACAAATTTGAAGTTTGAGAATAAGATTTTAATCCCTAATCATACCCTACGCTCTCTCGCTCAAGATTGGCATTACAAAAGATCAATTCCATATCCATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Pkinase_Tyr U-box

Protein Analysis

417

Amino Acids

47.66

Weight (kDa)

7.66

Isoelectric Point (pI)

47.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
PK_Tyr_Ser-Thr	PF07714	88 - 231	9.3e-24	Protein tyrosine and serine/threonine kinase
Pkinase	PF00069	89 - 231	2e-13	Protein kinase domain
U-box	PF04564	341 - 411	1.6e-14	U-box domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0014641)

Species	Orthologous Gene IDs
fragaria_vesca	FvH4_1g19540
malus_domestica	MD01G1040600.v1.1 MD15G1311200.v1.1
prunus_persica	Prupe.6G202800_v2.0.a1 Prupe.6G202800_v2.0.a1 Prupe.6G202800_v2.0.a1 Prupe.6G202800_v2.0.a1 Prupe.6G202800_v2.0.a1
rosa_chinensis	RchiOBHm_Chr2g0111051
rosa_laevigata	RLG00000017861
rosa_multiflora	Rmu_sc0003994.1_g000002
rosa_rugosa	Rorug02G0170700
rosa_samantha	Rh2AG223100 Rh2BG235100 Rh2CG226600 Rh2DG230900
rosa_wichuraiana	Rw2G017220

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	273
AccI	GTMKAC	2 cut(s)	54, 124
AciI	CCGC	6 cut(s)	526, 532, 542, 555, 634, 1079
AclWI	GGATC	3 cut(s)	979, 1061, 1074
AcoI	YGGCCR	3 cut(s)	210, 581, 827
AcsI	RAATTY	3 cut(s)	116, 221, 1156
AfaI	GTAC	4 cut(s)	78, 188, 683, 1097
AfiI	CCNNNNNNNGG	2 cut(s)	505, 574
AflIII	ACRYGT	1 cut(s)	49
AgsI	TTSAA	5 cut(s)	74, 116, 244, 982, 1162
AjuI	GAANNNNNNNTTGG	2 cut(s)	330, 362
AluBI	AGCT	8 cut(s)	28, 171, 302, 323, 364, 459, 858, 925
AluI	AGCT	8 cut(s)	28, 171, 302, 323, 364, 459, 858, 925
Alw26I	GTCTC	2 cut(s)	60, 182
AlwI	GGATC	3 cut(s)	979, 1061, 1074
Ama87I	CYCGRG	1 cut(s)	504
AoxI	GGCC	3 cut(s)	210, 581, 827
ApeKI	GCWGC	2 cut(s)	171, 539
ApoI	RAATTY	3 cut(s)	116, 221, 1156
Asp700I	GAANNNNTTC	1 cut(s)	345
AspS9I	GGNCC	3 cut(s)	22, 508, 890
AsuC2I	CCSGG	2 cut(s)	505, 506
AsuHPI	GGTGA	4 cut(s)	370, 596, 941, 1003
AsuII	TTCGAA	2 cut(s)	103, 438
AvaI	CYCGRG	1 cut(s)	504
AvaII	GGWCC	3 cut(s)	22, 508, 890
BalI	TGGCCA	2 cut(s)	212, 829
BamHI	GGATCC	1 cut(s)	1066
BanII	GRGCYC	1 cut(s)	669
BauI	CACGAG	1 cut(s)	641
BbsI	GAAGAC	1 cut(s)	427
BbvCI	CCTCAGC	1 cut(s)	608
BbvI	GCAGC	2 cut(s)	158, 551
BccI	CCATC	3 cut(s)	397, 622, 1079
BcnI	CCSGG	2 cut(s)	505, 506
BcoDI	GTCTC	2 cut(s)	60, 182
BisI	GCNGC	3 cut(s)	172, 540, 1080
BlsI	GCNGC	3 cut(s)	173, 541, 1081
BmcAI	AGTACT	1 cut(s)	78
Bme1390I	CCNGG	2 cut(s)	505, 506
Bme18I	GGWCC	3 cut(s)	22, 508, 890
BmeT110I	CYCGRG	1 cut(s)	504
BmgT120I	GGNCC	3 cut(s)	22, 508, 890
BmiI	GGNNCC	3 cut(s)	509, 892, 1068
BmrFI	CCNGG	2 cut(s)	505, 506
BmrI	ACTGGG	2 cut(s)	766, 1139
BmsI	GCATC	2 cut(s)	124, 537
BmuI	ACTGGG	2 cut(s)	766, 1139
BpiI	GAAGAC	1 cut(s)	427
BplI	GAGNNNNNCTC	2 cut(s)	536, 568
BpmI	CTGGAG	1 cut(s)	50
Bpu10I	CCTNAGC	2 cut(s)	354, 608
Bpu14I	TTCGAA	2 cut(s)	103, 438
BpuEI	CTTGAG	1 cut(s)	1197
BpuMI	CCSGG	2 cut(s)	505, 506
BsaBI	GATNNNNATC	2 cut(s)	244, 1181
BsaJI	CCNNGG	1 cut(s)	504
Bsc4I	CCNNNNNNNGG	2 cut(s)	505, 574
Bse1I	ACTGG	5 cut(s)	213, 264, 751, 761, 1134
Bse3DI	GCAATG	2 cut(s)	534, 830
Bse8I	GATNNNNATC	2 cut(s)	244, 1181
BseDI	CCNNGG	1 cut(s)	504
BseGI	GGATG	3 cut(s)	139, 382, 528
BseJI	GATNNNNATC	2 cut(s)	244, 1181
BseLI	CCNNNNNNNGG	2 cut(s)	505, 574
BseMI	GCAATG	2 cut(s)	534, 830
BseMII	CTCAG	3 cut(s)	193, 333, 622
BseNI	ACTGG	5 cut(s)	213, 264, 751, 761, 1134
BseRI	GAGGAG	4 cut(s)	212, 617, 935, 981
BseX3I	CGGCCG	1 cut(s)	581
BseXI	GCAGC	2 cut(s)	158, 551
BseYI	CCCAGC	2 cut(s)	417, 818
Bsh1285I	CGRYCG	3 cut(s)	526, 584, 634
BshFI	GGCC	3 cut(s)	212, 583, 829
BsiEI	CGRYCG	3 cut(s)	526, 584, 634
BsiHKCI	CYCGRG	1 cut(s)	504
BsiSI	CCGG	1 cut(s)	505
BsiWI	CGTACG	1 cut(s)	1095
BslFI	GGGAC	5 cut(s)	319, 479, 521, 719, 1007
BslI	CCNNNNNNNGG	2 cut(s)	505, 574
BsmAI	GTCTC	2 cut(s)	60, 182
BsmFI	GGGAC	5 cut(s)	319, 479, 521, 719, 1007
BsnI	GGCC	3 cut(s)	212, 583, 829
BsoBI	CYCGRG	1 cut(s)	504
Bsp119I	TTCGAA	2 cut(s)	103, 438
Bsp1286I	GDGCHC	1 cut(s)	669
Bsp143I	GATC	4 cut(s)	201, 971, 1066, 1232
BspACI	CCGC	6 cut(s)	526, 532, 542, 555, 634, 1079
BspANI	GGCC	3 cut(s)	212, 583, 829
BspCNI	CTCAG	3 cut(s)	192, 332, 621
BspLI	GGNNCC	3 cut(s)	509, 892, 1068
BspPI	GGATC	3 cut(s)	979, 1061, 1074
BspT104I	TTCGAA	2 cut(s)	103, 438
BsrDI	GCAATG	2 cut(s)	534, 830
BsrI	ACTGG	5 cut(s)	213, 264, 751, 761, 1134
BssECI	CCNNGG	1 cut(s)	504
BssMI	GATC	4 cut(s)	201, 971, 1066, 1232
BssSI	CACGAG	1 cut(s)	641
Bst2BI	CACGAG	1 cut(s)	641
Bst4CI	ACNGT	3 cut(s)	298, 333, 433
BstBI	TTCGAA	2 cut(s)	103, 438
BstC8I	GCNNGC	1 cut(s)	396
BstDEI	CTNAG	5 cut(s)	179, 319, 354, 472, 608
BstEII	GGTNACC	2 cut(s)	602, 1009
BstF5I	GGATG	3 cut(s)	139, 382, 528
BstKTI	GATC	4 cut(s)	204, 974, 1069, 1235
BstMAI	GTCTC	2 cut(s)	60, 182
BstMBI	GATC	4 cut(s)	201, 971, 1066, 1232
BstMCI	CGRYCG	3 cut(s)	526, 584, 634
BstMWI	GCNNNNNNNGC	1 cut(s)	1132
BstNSI	RCATGY	1 cut(s)	448
BstPI	GGTNACC	2 cut(s)	602, 1009
BstSCI	CCNGG	2 cut(s)	503, 504
BstV1I	GCAGC	2 cut(s)	158, 551
BstV2I	GAAGAC	1 cut(s)	427
BstX2I	RGATCY	2 cut(s)	971, 1066
BstYI	RGATCY	2 cut(s)	971, 1066
BstZI	CGGCCG	1 cut(s)	581
BsuRI	GGCC	3 cut(s)	212, 583, 829
BtgZI	GCGATG	1 cut(s)	1128
BtsCI	GGATG	3 cut(s)	139, 382, 528
BtsIMutI	CAGTG	2 cut(s)	271, 661
Cac8I	GCNNGC	1 cut(s)	396
Cfr13I	GGNCC	3 cut(s)	22, 508, 890
Cfr9I	CCCGGG	1 cut(s)	504
Csp6I	GTAC	4 cut(s)	77, 187, 682, 1096
CspCI	CAANNNNNGTGG	2 cut(s)	637, 672
CviQI	GTAC	4 cut(s)	77, 187, 682, 1096
DdeI	CTNAG	5 cut(s)	179, 319, 354, 472, 608
DpnI	GATC	4 cut(s)	203, 973, 1068, 1234
DpnII	GATC	4 cut(s)	201, 971, 1066, 1232
EaeI	YGGCCR	3 cut(s)	210, 581, 827
EagI	CGGCCG	1 cut(s)	581
EclXI	CGGCCG	1 cut(s)	581
Eco24I	GRGCYC	1 cut(s)	669
Eco47I	GGWCC	3 cut(s)	22, 508, 890
Eco52I	CGGCCG	1 cut(s)	581
Eco88I	CYCGRG	1 cut(s)	504
Eco91I	GGTNACC	2 cut(s)	602, 1009
EcoO109I	RGGNCCY	1 cut(s)	508
EcoO65I	GGTNACC	2 cut(s)	602, 1009
EcoT38I	GRGCYC	1 cut(s)	669
FaqI	GGGAC	5 cut(s)	319, 479, 521, 719, 1007
FauNDI	CATATG	1 cut(s)	1037
FblI	GTMKAC	2 cut(s)	54, 124
Fnu4HI	GCNGC	3 cut(s)	172, 540, 1080
FokI	GGATG	3 cut(s)	146, 369, 515
FriOI	GRGCYC	1 cut(s)	669
Fsp4HI	GCNGC	3 cut(s)	172, 540, 1080
GluI	GCNGC	3 cut(s)	172, 540, 1080
GsaI	CCCAGC	2 cut(s)	421, 822
GsuI	CTGGAG	1 cut(s)	50
HaeIII	GGCC	3 cut(s)	212, 583, 829
HapII	CCGG	1 cut(s)	505
HinfI	GANTC	1 cut(s)	730
HpaII	CCGG	1 cut(s)	505
HphI	GGTGA	4 cut(s)	370, 596, 941, 1003
Hpy166II	GTNNAC	3 cut(s)	55, 125, 189
Hpy188I	TCNGA	4 cut(s)	65, 182, 229, 938
Hpy188III	TCNNGA	3 cut(s)	249, 1052, 1214
Hpy8I	GTNNAC	3 cut(s)	55, 125, 189
Hpy99I	CGWCG	2 cut(s)	87, 689
HpyAV	CCTTC	7 cut(s)	13, 102, 146, 744, 782, 808, 1057
HpyCH4III	ACNGT	3 cut(s)	298, 333, 433
HpyCH4IV	ACGT	1 cut(s)	49
HpyCH4V	TGCA	2 cut(s)	448, 539
HpyF10VI	GCNNNNNNNGC	1 cut(s)	1132
HpyF3I	CTNAG	5 cut(s)	179, 319, 354, 472, 608
HpySE526I	ACGT	1 cut(s)	49
Kzo9I	GATC	4 cut(s)	201, 971, 1066, 1232
LmnI	GCTCC	3 cut(s)	419, 557, 922
Lsp1109I	GCAGC	2 cut(s)	158, 551
LweI	GCATC	2 cut(s)	124, 537
MaeII	ACGT	1 cut(s)	49
MaeIII	GTNAC	4 cut(s)	9, 292, 602, 1009
MalI	GATC	4 cut(s)	203, 973, 1068, 1234
MboI	GATC	4 cut(s)	201, 971, 1066, 1232
MboII	GAAGA	4 cut(s)	117, 353, 427, 731
MflI	RGATCY	2 cut(s)	971, 1066
MhlI	GDGCHC	1 cut(s)	669
MlsI	TGGCCA	2 cut(s)	212, 829
MluCI	AATT	7 cut(s)	116, 164, 221, 252, 993, 1156, 1236
MluNI	TGGCCA	2 cut(s)	212, 829
MlyI	GAGTC	1 cut(s)	724
Mox20I	TGGCCA	2 cut(s)	212, 829
MroXI	GAANNNNTTC	1 cut(s)	345
MscI	TGGCCA	2 cut(s)	212, 829
MseI	TTAA	6 cut(s)	315, 866, 875, 905, 987, 1181
MslI	CAYNNNNRTG	2 cut(s)	387, 1246
Msp20I	TGGCCA	2 cut(s)	212, 829
MspA1I	CMGCKG	2 cut(s)	28, 542
MspI	CCGG	1 cut(s)	505
MspR9I	CCNGG	2 cut(s)	505, 506
MwoI	GCNNNNNNNGC	1 cut(s)	1132
NciI	CCSGG	2 cut(s)	505, 506
NdeI	CATATG	1 cut(s)	1037
NdeII	GATC	4 cut(s)	201, 971, 1066, 1232
NlaIV	GGNNCC	3 cut(s)	509, 892, 1068
NmeAIII	GCCGAG	1 cut(s)	559
NmuCI	GTSAC	4 cut(s)	9, 292, 602, 1009
NspI	RCATGY	1 cut(s)	448
NspV	TTCGAA	2 cut(s)	103, 438
PdmI	GAANNNNTTC	1 cut(s)	345
Pfl23II	CGTACG	1 cut(s)	1095
PflFI	GACNNNGTC	1 cut(s)	59
PkrI	GCNGC	3 cut(s)	173, 541, 1081
PleI	GAGTC	1 cut(s)	724
PpsI	GAGTC	1 cut(s)	724
PpuMI	RGGWCCY	1 cut(s)	508
PsiI	TTATAA	1 cut(s)	273
Psp5II	RGGWCCY	1 cut(s)	508
PspEI	GGTNACC	2 cut(s)	602, 1009
PspFI	CCCAGC	2 cut(s)	417, 818
PspLI	CGTACG	1 cut(s)	1095
PspN4I	GGNNCC	3 cut(s)	509, 892, 1068
PspPI	GGNCC	3 cut(s)	22, 508, 890
PspPPI	RGGWCCY	1 cut(s)	508
PsuI	RGATCY	2 cut(s)	971, 1066
PsyI	GACNNNGTC	1 cut(s)	59
PvuII	CAGCTG	1 cut(s)	28
RsaI	GTAC	4 cut(s)	78, 188, 683, 1097
RsaNI	GTAC	4 cut(s)	77, 187, 682, 1096
RseI	CAYNNNNRTG	2 cut(s)	387, 1246
SaqAI	TTAA	6 cut(s)	315, 866, 875, 905, 987, 1181
SatI	GCNGC	3 cut(s)	172, 540, 1080
Sau3AI	GATC	4 cut(s)	201, 971, 1066, 1232
Sau96I	GGNCC	3 cut(s)	22, 508, 890
ScaI	AGTACT	1 cut(s)	78
SchI	GAGTC	1 cut(s)	724
ScrFI	CCNGG	2 cut(s)	505, 506
SduI	GDGCHC	1 cut(s)	669
SfaNI	GCATC	2 cut(s)	124, 537
SfuI	TTCGAA	2 cut(s)	103, 438
SinI	GGWCC	3 cut(s)	22, 508, 890
SmaI	CCCGGG	1 cut(s)	506
SmiMI	CAYNNNNRTG	2 cut(s)	387, 1246
SmlI	CTYRAG	1 cut(s)	1212
SmoI	CTYRAG	1 cut(s)	1212
Sse9I	AATT	7 cut(s)	116, 164, 221, 252, 993, 1156, 1236
SsiI	CCGC	6 cut(s)	526, 532, 542, 555, 634, 1079
SspI	AATATT	1 cut(s)	985
StyD4I	CCNGG	2 cut(s)	503, 504
TaaI	ACNGT	3 cut(s)	298, 333, 433
TaiI	ACGT	1 cut(s)	52
TaqI	TCGA	5 cut(s)	103, 438, 617, 630, 799
TasI	AATT	7 cut(s)	116, 164, 221, 252, 993, 1156, 1236
TatI	WGTACW	2 cut(s)	76, 186
TauI	GCSGC	1 cut(s)	1082
Tru1I	TTAA	6 cut(s)	315, 866, 875, 905, 987, 1181
Tru9I	TTAA	6 cut(s)	315, 866, 875, 905, 987, 1181
TscAI	CASTG	2 cut(s)	271, 661
TseFI	GTSAC	4 cut(s)	9, 292, 602, 1009
TseI	GCWGC	2 cut(s)	171, 539
Tsp45I	GTSAC	4 cut(s)	9, 292, 602, 1009
TspDTI	ATGAA	7 cut(s)	51, 59, 501, 587, 989, 1024, 1076
TspMI	CCCGGG	1 cut(s)	504
TspRI	CASTG	2 cut(s)	271, 661
Tth111I	GACNNNGTC	1 cut(s)	59
VpaK11BI	GGWCC	3 cut(s)	22, 508, 890
XapI	RAATTY	3 cut(s)	116, 221, 1156
XceI	RCATGY	1 cut(s)	448
XmaI	CCCGGG	1 cut(s)	504
XmiI	GTMKAC	2 cut(s)	54, 124
XmnI	GAANNNNTTC	1 cut(s)	345
ZrmI	AGTACT	1 cut(s)	78

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.